Journal of Cell Science and Therapy最新文献

{"title":"Human Retroviruses and Soluble CD30 Levels of Patients","authors":"S. Takemoto","doi":"10.4172/2157-7013.1000184","DOIUrl":"https://doi.org/10.4172/2157-7013.1000184","url":null,"abstract":"Human T-cell leukemia virus type 1 infection makes CD4+ T cells transformed into leukemic cells, whereas human immunodeficiency virus make them destroyed. Interestingly, activation of CD30 led to the induction of apoptotic death of anaplastic large cell lymphoma cells, while Hodgkin’s lymphoma cells, which constitutively express NF-κB, were not susceptible to CD30-induced apoptosis. Such a pleiotropic effect of CD30 signaling is dependent on cell type, B-cell or T-cell, and probably different activation status of NF-κB, constitutive or inducible.Accordingly, CD30 triggering may be responsible for the enhanced retroviral replication and T cell death under the influence of T cell activation and/or NF-κB activation status, showing high levels of sCD30.","PeriodicalId":150547,"journal":{"name":"Journal of Cell Science and Therapy","volume":"41 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134235989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impaired Cardiac Function in Cardiac-Specific SIRT1-Deficient Mice","authors":"Feng Cao","doi":"10.4172/2157-7013.1000I101","DOIUrl":"https://doi.org/10.4172/2157-7013.1000I101","url":null,"abstract":"Cardiac specific SIRT1 knockout mice were generated through the Cre/loxP system. Briefly, loxP-SIRT1 mice and Myh6-Cre mice were crossed to get SIRT1+/- mice.","PeriodicalId":150547,"journal":{"name":"Journal of Cell Science and Therapy","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115247266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Induced Pluripotent Stem Cells for the Treatment of Hemophilia A","authors":"B. C. Joseph, M. Rao","doi":"10.4172/2157-7013.1000E119","DOIUrl":"https://doi.org/10.4172/2157-7013.1000E119","url":null,"abstract":"Factor VIII, one of the most complex proteins known, plays a major role in blood coagulation pathway. Defects in factor VIII protein result in hemophilia A, a severe bleeding disorder. Plasma derived factor VIII or recombinant factor VIII has been used extensively for treating hemophilia A patients. Number of attempts at gene therapy for hemophilia A has failed for various unknown/not much studied reasons including immune rejection. Here, the progress that has been made in establishing iPSC-based disease models and the potentials of iPSC technology for personalized medicine and cell therapy for hemophilia A are reviewed. The challenges of iPSC technology are also briefly discussed.","PeriodicalId":150547,"journal":{"name":"Journal of Cell Science and Therapy","volume":"118 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116353146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Connection between Human and Animal Health","authors":"C. Lavrador","doi":"10.4172/2157-7013.1000E118","DOIUrl":"https://doi.org/10.4172/2157-7013.1000E118","url":null,"abstract":"The strong connection between human and animal health. How far can we go with the One Health approach? The Zoobiquity revelation.","PeriodicalId":150547,"journal":{"name":"Journal of Cell Science and Therapy","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117088289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Pressure, Surface Tension and Rest Potential on Osteo Intra Organelle Nanoporation in an Advanced Micro Chip","authors":"S. Sarkar, R. Mahapatra","doi":"10.4172/2157-7013.1000187","DOIUrl":"https://doi.org/10.4172/2157-7013.1000187","url":null,"abstract":"We report the effect of pressure, surface tension and rest potential on intra organelle nanoporation of multi layer osteoblast cell placed in a 3D non uniform micro fluidic chip composed of bi metallic heterogeneous micro electrode under the influences of smart control FPGA based Pico pulse generator. It is exposed that all above parameters are not uniformly distributed over the surface and affects the pore free energy. Pore radius and density are symmetric about the equator with the same value at both poles of the cell. In addition, the value of the rest potential and surface tension affect membrane potential around the cell because the electro oration current is several orders of magnitude larger than the ionic current that supports the rest potential and surface tension. Complete resealing to pre shock conditions requires specific surface tension and pressure.","PeriodicalId":150547,"journal":{"name":"Journal of Cell Science and Therapy","volume":"61 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134152042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stem Cell Implant Composites for Periodontal Tissue Revival","authors":"S. Dravida, L. Krishna","doi":"10.4172/2157-7013.1000183","DOIUrl":"https://doi.org/10.4172/2157-7013.1000183","url":null,"abstract":"Owing to good biocompatibilities, resistance to corrosion and mechanical properties, Titanium (Ti) implants are popularly used in dentistry. The fundamental aspect in the performance of an implant is the mechanical and biological behaviour of its interface with the surrounding micro-environment of the periodontium","PeriodicalId":150547,"journal":{"name":"Journal of Cell Science and Therapy","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126606592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aflatoxins and Hepatitis B, C Viral Associated Hepatocarcinogenesis","authors":"H. Qureshi, S. Ali, M. Iqbal, A. Siddiqui, N. Khan, S. Hamid","doi":"10.4172/2157-7013.1000179","DOIUrl":"https://doi.org/10.4172/2157-7013.1000179","url":null,"abstract":"Hepatocellular carcinoma is a serious human disease with fatal consequences. The most distressing aspect of hepatocellular carcinoma is the limited improvement in mortality (mortality rate of more than 90%). At present, the underlying molecular mechanisms are not well understood and treatment options are often of limited efficacy. This review presents our current understanding of the burden of hepatocellular carcinoma on human health, pathogenesis and pathophysiology, and molecular mechanisms associated with the disease, as well as our knowledge of the physical barriers, cellular mechanisms and molecular elements that may be targets for therapeutic interventions and/ or the development of preventative measures. As the proposed findings present a major risk to public health, it is hoped that robust intervention measures will be introduced for aflatoxins monitoring and reduction in diet.","PeriodicalId":150547,"journal":{"name":"Journal of Cell Science and Therapy","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117071831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Percutaneous injection of bone cement (Cementoplasty) for the treatment of symptomatic subchondral cysts","authors":"A. Bertrand","doi":"10.4172/2157-7013.1000176","DOIUrl":"https://doi.org/10.4172/2157-7013.1000176","url":null,"abstract":"Objective: To evaluate percutaneous computed tomography (CT) and fluoroscopy-guided injection of bone cement for the treatment of symptomatic subchondral cysts of the appendicular skeleton. \u0000Methods: A single-center prospective study involving 13 consecutive patients with symptomatic subchondral cysts was done (8 women, 5 men). The average age was 67 years. Patients were treated by percutaneous CTguided injection of bone cement into the subchondral cysts. Surgical treatment was not indicated or not wished by the patients who underwent cementoplasty. The lesions were all located in weight-bearing bones, involving the femoral head, femoral condyle, tibial plateau, talus and calcaneus respectively and consisting of subchondral cysts resulting from degenerative lesions or aseptic osteonecrosis. The clinical course of pain was evaluated using the Visual Analog Scale (VAS) before treatment, at one month and three months after treatment, with long-term follow-up from 2 months to 43 months (average follow-up: 22 months). \u0000Results: Patient follow-ups in our series show supportive results within 13 patients, 12 patients were satisfied with a long-lasting result after the procedure had been performed, and would recommend the intervention to relatives. The average evaluation of pain was 8/10 (SD: 0.49) before treatment, 3/10 (SD: 0.66) one month after treatment and 1/10 (SD: 0,60) three months after treatment. Our results show a significant decrease of the pain felt by patients between before procedure and one month after the procedure (p=0,002), before procedure and three months after the procedure (p=0,002), one month after the procedure and three months after the procedure (p=0.011). There were no immediate or delayed complications. We observed one asymptomatic para-articular cement leakage at the knee. One patient was not relieved after the procedure and underwent hip surgery. \u0000Conclusions: Percutaneous injection of bone cement under CT and fluoroscopy guidance seems to be an effective and safe procedure in the treatment of symptomatic subchondral cysts with a significant decrease of patient’s pain and a mini-invasive approach compared to classical surgical treatment. Thus we recommend that it should be considered as a first choice of treatment for symptomatic subchondral cysts.","PeriodicalId":150547,"journal":{"name":"Journal of Cell Science and Therapy","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130105975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Malignant Progression of Glioblastoma","authors":"Y. Iwadate","doi":"10.4172/2157-7013.1000182","DOIUrl":"https://doi.org/10.4172/2157-7013.1000182","url":null,"abstract":"Glioblastoma (GBM) is the most devastating type of human cancers with a median survival time ranging from 12-18 months following maximal therapies [1]. Glioma is classified into four malignancy grades according to histological features; the low-grade tumors including WHO Grade I (localized gliomas) and WHO Grade II (diffuse astrocytoma or oligodendrogliomas), and the high-grade tumors including WHO Grades III (anaplastic gliomas) and WHO Grade IV (GBM).","PeriodicalId":150547,"journal":{"name":"Journal of Cell Science and Therapy","volume":"50 1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114348482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fibrin-Targeting, Peptide Amphiphile Micelles as Contrast Agents for Molecular MRI","authors":"E. J. Chung, F. Pineda, K. Nord, G. Karczmar, Seonghyun Lee, M. Tirrell","doi":"10.4172/2157-7013.1000181","DOIUrl":"https://doi.org/10.4172/2157-7013.1000181","url":null,"abstract":"Magnetic resonance imaging (MRI) provides a nonionizing and safe imaging modality for cancer diagnostics. Here, we took advantage of the fibrin deposition that is characteristic of tumors and the ability to incorporate multiple functionalities within peptide amphiphile micelles (PAMs) to design a new class of contrast agents for molecular MRI. We report on synthesis, formulation, and preliminary tests for MRI of spherical PAMs that were self-assembled by combining 18:0 PE-DTPA (Gd) and peptide amphiphiles containing the fibrin-binding pentapeptide, cysteinearginine- glutamic acid-lysine-alanine, or CREKA. Conjugation of the CREKA peptide to micelles increased the average particle size and zeta potential, and T1 relaxivities of CREKA-Gd PAMs (per mmol of Gd) were found to be comparable to contrast agents which are used routinely in clinical settings at 1.5T and 3T. Moreover, when murine fibroblasts were cultured with CREKA-Gd PAMs, no cytotoxicity was demonstrated and cell viability was comparable to that of PBS-treated controls for up to 3 days.Our study provides proof-of-concept of CREKA-Gd PAMs as contrast agents for molecular MRI, and a facile strategy for incorporating contrast agents and bioactive molecules into nano carriers to develop safe, targeted diagnostic carriers for clinical application.","PeriodicalId":150547,"journal":{"name":"Journal of Cell Science and Therapy","volume":"99 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124098765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}