Journal of Cell Science and Therapy最新文献

{"title":"A Paradigm of Reactive Oxygen Species and Programed Cell Death in Plants","authors":"K. Nath, Yan Lu","doi":"10.4172/2157-7013.1000202","DOIUrl":"https://doi.org/10.4172/2157-7013.1000202","url":null,"abstract":"Plants are continuously exposed to a large variety of biotic and abiotic stresses during their successive stages of development [1-4]. Living organisms have different endogenous strategies to acclimate to a series of adverse environmental conditions. Animals can easily move from one place to another to avoid challenging environmental stresses.","PeriodicalId":150547,"journal":{"name":"Journal of Cell Science and Therapy","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126497940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diffusion Tensor Imaging Predicts Motor Functional Outcome after Acute Hypertensive Intracerebral Hemorrhage","authors":"I. Han, Jin-soo Kim, B. Kim, Soo‐Hong Lee","doi":"10.4172/2157-7013.1000203","DOIUrl":"https://doi.org/10.4172/2157-7013.1000203","url":null,"abstract":"Early evaluation of the corticospinal tract (CST) is critical for patients with acute hypertensive intracerebral hemorrhage (ICH) to predict long-term motor functional outcome. Therefore, we investigated motor functional outcome of an ICH using diffusion tensor imaging (DTI) in patients with hypertensive ICH. Thirty six patients with hemiparesis due to hypertensive ICH underwent DTI within 3 days after onset. Fractional anisotropy (FA) was measured within the CST at levels of the posterior limbs of the internal capsules, and motor impairment was assessed on admission and 8 weeks after ICH by manual muscle test. The correlation between FA ratio and improvement of motor function was analyzed by Pearson’s correlation analysis. The FA ratio from the initial DTI showed a correlation with motor function improvement. The amount of hematoma correlated with motor function on admission, but did not show correlation with the degree of motor recovery. Therefore, FA ratio analysis calculated from FA values of DTI could be a prognostic factor of motor function improvement in patients with hypertensive ICH.","PeriodicalId":150547,"journal":{"name":"Journal of Cell Science and Therapy","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115473204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Live Splenic Ectopic Pregnancy","authors":"Youxin Ji, Ketao Ian, Keke Nie, Shi-chao Liu, Chunling Zhang, Rong Wang","doi":"10.4172/2157-7013.1000201","DOIUrl":"https://doi.org/10.4172/2157-7013.1000201","url":null,"abstract":"Splenic ectopic pregnancy is very rare, it is easy to be negligence by doctors and the outcome is ominous if not treated promptly. Here we reported an extremely rare case happened in a young Chinese woman. She was admitted in to hospital by hypovolemic shock. A live splenic ectopic pregnancy was diagnosed by ultrasound. Laparotomy with splenectomy was performed and histology confirmed the diagnosis. Early diagnosis of live splenic ectopic pregnancy is critical important for save patient's life, doctors should aware this disease.","PeriodicalId":150547,"journal":{"name":"Journal of Cell Science and Therapy","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133015512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and Characterization of CD133 Positive Stem Cell from Human Kidney with Renal Cell Carcinoma","authors":"M. Abbasi, S. Heidari-keshel, R. Zahed, G. Behrouzi, R. Roozafzoon, Sara Aghazadeh, Leilia Aghajanpour, M. Bashtar, A. Khoshzaban","doi":"10.4172/2157-7013.1000200","DOIUrl":"https://doi.org/10.4172/2157-7013.1000200","url":null,"abstract":"Background: Stem cells are one of the rare cell populations in each tissue, whose presence is proven in different types of tissues in the human body. The purpose of this study was to carry out simultaneous investigation into renal stem cells, which are similar to stem cell populations (CD133+) in normal part of the human kidney of a patient afflicted with renal cell carcinoma. \u0000Methods: After kidney dissection, its normal part was divided into papilla, medulla, and cortex. Then, the stem cells of each part were separately isolated. Two cell groups (A and B) were considered. Group A contained the MACS-isolated CD133+ cells and group B consisted of the cells, which had not been sorted by any markers. After enzymatic digestion, all isolated cells in both groups were cultured. The cells of each part were analyzed by flowcytometry, proliferation assay, karyotyping and gene expression. \u0000Results: The results of the present study show that CD133+ cells are renal stem cells and they are found in every three parts of the kidney, but their frequency is higher in papilla. The cells in group B expressed cell surface marker CD44 significantly. The high expression of OCT 3/4, NANOG, SOX2 and SCA-1 genes was seen in both groups, but the expression of REX1 gene in group A was 5 times more than in group B. \u0000Conclusion: It seems that CD133+ cells are the most original cell population found in papilla of human kidney with high frequency. The finding has explored a new horizon towards appropriate selection of stem cells subpopulations (CD133+ cell from healthy part of renal carcinoma) to be used in cellular therapeutic approaches in more defined manner.","PeriodicalId":150547,"journal":{"name":"Journal of Cell Science and Therapy","volume":"2013 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127421267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nectar Mix Functional Based on Amazonian Fruits","authors":"M. Faber, L. Yuyama","doi":"10.4172/2157-7013.1000197","DOIUrl":"https://doi.org/10.4172/2157-7013.1000197","url":null,"abstract":"Background: Functional foods are characterized by offering several health benefits beyond the nutritional value inherent in their chemical composition. Fruits are good suppliers of minerals, vitamins, fiber, and water. Some fruits can even provide small amounts of carbohydrates among other ingredients. Several fruit plants in the Amazon region have great economic potential due to their nutritious value. Fruits that are rich sources of dietary fiber, vitamin C and mineral elements. Implementation of Amazonian fruits cupuacu (Theobroma grandiflorum), camu-camu (Dunal Myrciaria dubia McVaugh), cubiu (Solanum sessiliflorum Dunal), adjusting the proportions of the objectives of the functional product of biotechnology, aimed at producing a product with low energy and high nutritional value. \u0000Subjects/Methods: The preparation consisted of chemical and physicochemical analyses composition, analysis of inorganic constituents of the fruits, product formulation, microbiological analysis of formulated products, and sensory analysis. The sensory analysis of nectar was divided into nine sets, where for each concentration of pulp was determined the best concentration of sucralose. The experimental design used the factor 33 and descriptive statistics. \u0000Results: We developed a low calorie bioactive dietary nectar mix, sweetened with sweetener sucralose. The microbiological analysis of the formulation showed: Coliform bacteria, 0/ml; mesophylls (CPP) <10UFC/ml; psychrophilic (CPP) <10UFC/ml, molds and yeasts <10UFC/ml, and some Salmonella sp. The result was a lowenergy product, 59.441 kj/100 ml, and the nutritional assessment shows: Potassium 103.23 mg/100 ml; Calcium 7.15 mg/100 ml; Phosphorus 7.30 mg/100 ml; Fibers 1.45 g/100 ml; Vitamin B3 0.43 mg/100 ml; Vitamin C 260.83 mg/100 ml. The experimental design used the factor 33 and descriptive statistics showed confidence interval 95% and P value 0.03 and 0.58. \u0000Conclusion: This product can be consumed by almost all the population assisting in the nutrition of children, youth, adults, elders, and even during pregnancy. It can also to be assigned of diet and still be recommended to individuals with restriction of sugars.","PeriodicalId":150547,"journal":{"name":"Journal of Cell Science and Therapy","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117099535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human Embryonic Stem Cell Therapy in Cerebral Palsy Children with Cortical Visual Impairment: A Case Series of 40 Patients","authors":"G. Shroff, Lopamudra Das","doi":"10.4172/2157-7013.1000189","DOIUrl":"https://doi.org/10.4172/2157-7013.1000189","url":null,"abstract":"Background: Use of human embryonic stem cells (hESCs) has been explored for treatment of cortical visual impairment (CVI) in children with cerebral palsy (CP). \u0000Objectives: The study evaluated efficacy and safety of hESC therapy in 40 CP children with CVI. Methods: The study had four treatment phases (T1, T2, T3, and T4) separated by gap phases. Patients were evaluated for visual impairment using Nutech Functional Scores (NFS). \u0000Results: Of 40 patients included in study, 8 had NFS level \"1\" (were blind/ had no perception of light); 16 had level\"2\" (perception of light); 10 had level \"3\" (could identify blurred images); and 6 had level \"4\" (could see objects up to a distance of 25 cm from the eye). After therapy, 27 patients gained normal vision; 10 patients could see objects 25 cm from the eye; 2 patients could see blurred images; and 1 had perception of light. Overall, 39 patients showed improvement in NFS by at least one level. In SPECT scan, 2 patients had normal perfusion, 18 had significant improvement and 3 had moderate improvement. \u0000Conclusion: Use of hESC therapy in patients with CVI showed beneficial results for treatment of CVI in children with CP.","PeriodicalId":150547,"journal":{"name":"Journal of Cell Science and Therapy","volume":"65 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123967590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Renal Calculus Complicated with Squamous Cell Carcinoma of Renal Pelvic: Report of Two Cases","authors":"Jiantao Xiao, Jing Tan, Leye He, G. Yin","doi":"10.4172/2157-7013.1000191","DOIUrl":"https://doi.org/10.4172/2157-7013.1000191","url":null,"abstract":"Squamous cell carcinoma (SCC) of the renal pelvis is an uncommon tumor, which is usually associated with infection and long standing renal calculus. The neoplasm is highly aggressive, so it is at advanced stage and has a poor outcome when diagnosed. We present two cases who had diseases of kidney stone complicated with mass of renal pelvis. Both patients were operated with radical nephrectomy subsequently. The histopathology report showed that the tumors of renal pelvis were SCC. When come across a case of chronic renal calculi, we should pay attention to whether it exists neoplasm because the co-existing latent mass may bring about misdiagnosing and effect the patient’s treatment plan and prognosis.","PeriodicalId":150547,"journal":{"name":"Journal of Cell Science and Therapy","volume":"50 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130451928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-cancer Effects of Glypican-3 on Huh-7 Hepatocellular Carcinoma Cells","authors":"Ji-Yu Wen, Xiaojun Wen, Jinju Wang, Y. Shu, Z. Qiu, Zhong‐Kao Liu, Ran Li, Guo-fang Zeng, S. Bao, Huilai Miao, Yanfang Chen, Ming-yi Li","doi":"10.4172/2157-7013.1000186","DOIUrl":"https://doi.org/10.4172/2157-7013.1000186","url":null,"abstract":"Aim: Previous studies have suggested Glypican-3 (GPC3) could be a valuable diagnostic marker for hepatocellular carcinoma. This study examined the effects of overexpression of GPC3 on Huh-7 hepatoma cells. \u0000Methods: We constructed a recombinant plasmid vector pcDNA3.1 (+)-GPC3 for GPC3 overexpression studies in Huh-7 cells. RT-PCR and Western blotting were used to confirm GPC3 gene expression. Cell proliferation was evaluated by 5-ethynyl-2-deoxyuridine (EdU) incorporation assay. Cell cycle progression and apoptosis were determined by flow cytometry using propidium iodide (PI) and Annexin V-FITC/PI staining, respectively. Cell migration and invasion were examined by Boyden Transewll and Matrigel assays. \u0000Results: GPC3 overexpression effectively inhibited proliferation, induced cell cycle arrest at S phase and increased apoptosis in Huh-7 cells. Furthermore, GPC3 overexpression significantly inhibited the migration and invasion ability of Huh-7 cells. Conclusion: Our results demonstrate that GPC3 could be a new therapeutic target for hepatocellular carcinoma","PeriodicalId":150547,"journal":{"name":"Journal of Cell Science and Therapy","volume":"48 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128121809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Is Aflatoxin B1 A Biomarker for Pathogenic Potential of Aspergillus flavus","authors":"H. Qureshi, S. Hamid, S. Ali, Javeria Anwar, M. Iqbal, N. Khan","doi":"10.4172/2157-7013.1000188","DOIUrl":"https://doi.org/10.4172/2157-7013.1000188","url":null,"abstract":"Given the opportunity and the host immune status, Aspergillus flavus can produce aspergillosis affecting various body organs, while its toxin, Aflatoxin B1 (AFB1) has been implicated as a carcinogen in hepatocellular carcinoma. Based on previous findings, A. flavus can be divided into two groups, (i) isolates that can synthesize AFB1, and (ii) isolates unable to produce AFB1. The aim of this study was to assess whether AFB1 can be used as a marker to differentiate clinical and non-clinical isolates of A. flavus. Representative clinical isolates were obtained from patients while non-clinical isolates were obtained from the environment. Isolates were identified as A. flavus using selective media. AFB1 production was assessed through cultural assays and genes for aflatoxin production, aflR and aflS, were amplified using PCR. Conditioned media and methanol extract of A. flavus isolates were prepared and tested for AFB1 presence using Liquid Chromatography-Mass Spectrometry (LC-MS). Additionally, conditioned media and extract were tested for their cytotoxic effects on primary human brain microvascular endothelial cells (HBMEC) and immortalized human heptaoma cells (Huh7). Both clinical and non-clinical isolates of A.flavus exhibited aflatoxin production, albeit some clinical isolates produced excessive AFB1 (up to 15785 ng/mL). Importantly, A. flavus isolates produced higher levels of AFB1, exhibited increased host cell cytotoxicity, whereas strains exhibiting negligible amount of aflatoxin exhibited minimal cytotoxic effects suggesting AFB1 as a marker for pathogenic potential of A. flavus. The ability of aflatoxigenic A. flavus to produce host cell death in primary cells raises additional concern for patients suffering from A. flavus infection.","PeriodicalId":150547,"journal":{"name":"Journal of Cell Science and Therapy","volume":"143 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134335469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Formation of Cell-To-Cell Connection between Bone Marrow Cells and Isolated Rat Cardiomyocytes in a Cocultivation Model","authors":"J. Skopalík, M. Pásek, Milan Rychtarik, Z. Kořístek, E. Gabrielova, P. Scheer, P. Matejovič, M. Modriansky, M. Klabusay","doi":"10.4172/2157-7013.1000185","DOIUrl":"https://doi.org/10.4172/2157-7013.1000185","url":null,"abstract":"Aims: Limited regenerative potential of cardiomyocytes (CMs) causes irreversible changes in heart tissue during pathological processes. However bone marrow mononuclear cells (BM-MNCs) can migrate to this tissue, incorporate to the area of dead or missing myocytes, and improve the global heart function. The mechanism of BMMSCs’ incorporation and interaction with CMs is not clear. Our aim was to create an in vitro model which would enable to study the interaction of BM-MNCs with CMs and to make a microscopy description of these interactions. \u0000Methods and Results: CMs were isolated from adult and newborn rats. BM-MNCs were isolated from bone marrow. BM-MNCs were added to the myocyte culture. Cell-to-cell adherence and Cx43 expression were evaluated by fluorescence microscopy, Ca2+ transients were evaluated in cardiomyocyte-BMC communication under electrical stimulation by fluo-4 fluorescence measurement. Analysis of calcein transport from BM-MNCs to CMs was performed using fluorescence microscopy. \u0000Conclusions: The adherence of BM-MNCs to CMs occurred quickly and was stable. Cx43 was detected in contact zones between BM-MNCs and CMs; pairs which displayed Cx43 positivity represented less than 1% from all BM-MNC-cardiomyocyte pairs in the coculture. Conductive structures between CMs and BM-MNCs were formed and verified by imaging calcein transfer and synchronous Ca2+ transients.","PeriodicalId":150547,"journal":{"name":"Journal of Cell Science and Therapy","volume":"399 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122533898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}