Journal of Cell Science and Therapy最新文献_第8页

Ethical Principles in the Field of Tissue Banking 组织库领域的伦理原则

Journal of Cell Science and Therapy Pub Date : 2015-06-28 DOI: 10.4172/2157-7013.1000208

J. M. Pedraza

引用次数: 0

Targeting Glioma Stem Cells for Therapy: Perspectives and Challenges 靶向胶质瘤干细胞治疗:观点和挑战

Journal of Cell Science and Therapy Pub Date : 2015-06-18 DOI: 10.4172/2157-7013.1000207

Shaofang Wu, N. Saito, W. Yung, D. Koul

引用次数: 1

2-0, 3-0 Desulfated Heparin does not Affect Radiation Injury Induced Mortality but Reduces Radiation Combined Skin-burn Injury Induced Survival in Mice 2- 0,3 -0去硫肝素不影响辐射损伤诱导的死亡率，但降低辐射合并皮肤烧伤诱导的小鼠存活率

Journal of Cell Science and Therapy Pub Date : 2015-06-18 DOI: 10.4172/2157-7013.1000209

A. Islam, D. L. Bolduc, M. Zhai, S. Hobbs, Joshua M. Swift

{"title":"2-0, 3-0 Desulfated Heparin does not Affect Radiation Injury Induced Mortality but Reduces Radiation Combined Skin-burn Injury Induced Survival in Mice","authors":"A. Islam, D. L. Bolduc, M. Zhai, S. Hobbs, Joshua M. Swift","doi":"10.4172/2157-7013.1000209","DOIUrl":"https://doi.org/10.4172/2157-7013.1000209","url":null,"abstract":"Many radiation events have involved a high incidence of radiation combined injuries. Victims of radiation events succumb to serious infections as a consequence of bacterial translocation and sepsis. Exacerbation of the risk of infection by radiation combined burn injury (RCBI) further heightens vulnerability. There are currently no suitable countermeasures that exist for RCBIs. We evaluated 2-0, 3-0 desulfated heparin (ODSH), an anti-inflammatory and anticoagulant agent as a potential countermeasure to RCBI. Female B6D2F1/J mice (12-week) were subjected to 9.5 Gy (LD70/30 for RCBI) whole-body bilateral 60Co gamma-photon radiation (0.4 Gy/min), followed by dorsal skin burn injury under anesthesia (∼15% total-body-surface area burn). Mice were injected subcutaneously with ODSH (25 mg/kg every 12 h; days 1-2 and 17.5 mg/kg every 12 h; days 3-7) or vehicle (sterile saline of equal volume) for 7 days post-injury and further administered topical gentamicin (0.1% cream; days 1-10) and oral levofloxacin (100 mg/kg; days 3-16). Mice were euthanized on day 30 following water consumption, body mass and survival analysis. Our data showed ODSH had no effect on radiation injury (RI)-induced mortality (45% ODSH vs. 45% VEH; n=20). However interestingly, ODSH treatment significantly reduced survival after RCBI (12% ODSH vs. 41% VEH; n=22, p<0.05). Furthermore, ODSH did not affect water consumption or body mass accrual after RI or RCBI. ODSH was not able to counteract the negative alterations in hematology, splenocytes, or bone marrow cell counts after RI or RCBI. These data illustrate that ODSH in combination with antibiotic treatments, may not be a mitigating countermeasure for RCBI.","PeriodicalId":150547,"journal":{"name":"Journal of Cell Science and Therapy","volume":"77 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114919241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Detection of Prostate Cancer by Methylation Analysis of Plasminogen Activator Inhibitor-1 in Serum DNA 血浆DNA中纤溶酶原激活物抑制剂-1甲基化分析在前列腺癌分子检测中的应用

Journal of Cell Science and Therapy Pub Date : 2015-06-18 DOI: 10.4172/2157-7013.1000210

Brit Nacke, Albert Hagelgans, S. Fuessel, M. Wirth, G. Siegert, M. Menschikowski

{"title":"Molecular Detection of Prostate Cancer by Methylation Analysis of Plasminogen Activator Inhibitor-1 in Serum DNA","authors":"Brit Nacke, Albert Hagelgans, S. Fuessel, M. Wirth, G. Siegert, M. Menschikowski","doi":"10.4172/2157-7013.1000210","DOIUrl":"https://doi.org/10.4172/2157-7013.1000210","url":null,"abstract":"Background: Epigenetic modifications are common in malignant tissues. Here we analyzed the methylation degree of plasminogen activator inhibitor 1 (PAI-1) gene in comparison to the methylation of glutathione-stransferase- π (GSTP1) gene in prostate cancer (PCa). \u0000Methods: PAI-1 hypermethylation was studied using methylation-sensitive high resolution melting (MS-HRM) analysis of bisulfite-modified DNA and methylation-sensitive restriction endonuclease based quantitative PCR (MSRE-qPCR) on unmodified genomic DNA. \u0000Results: Data, obtained by these two methods, correlate closely. Methylation levels of PAI-1 analyzed in tissue specimens and serum samples were nearly similar. The diagnostic performance of the MSRE-qPCR assay characterized by AUC values was 0.944 and 0.937 for PAI-1 and GSTP1, respectively. Combination of both markers resulted in higher values of AUC, sensitivity and specificity. \u0000Conclusion: MSRE-qPCR based methylation analysis of PAI-1 gene and especially - in combination with GSTP1 gene may have potential as epigenetic marker of PCa in biological fluids.","PeriodicalId":150547,"journal":{"name":"Journal of Cell Science and Therapy","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116758484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interstitial Cells of Cajal and the Promise of Single Cell Molecular Analysis Cajal的间质细胞及其单细胞分子分析的前景

Journal of Cell Science and Therapy Pub Date : 2015-04-30 DOI: 10.4172/2157-7013.1000E123

Raul Loera-Valencia

引用次数: 1

Bacterial Paediatric Meningitis Laboratory Diagnosis. 细菌性儿科脑膜炎实验室诊断。

Journal of Cell Science and Therapy Pub Date : 2015-04-22 DOI: 10.4172/2157-7013.1000204

M. Gudza-Mugabe, Robertson, Mapingure Mp, S. Mtapuri-Zinyowera, R. T. Mavenyengwa

{"title":"Bacterial Paediatric Meningitis Laboratory Diagnosis.","authors":"M. Gudza-Mugabe, Robertson, Mapingure Mp, S. Mtapuri-Zinyowera, R. T. Mavenyengwa","doi":"10.4172/2157-7013.1000204","DOIUrl":"https://doi.org/10.4172/2157-7013.1000204","url":null,"abstract":"Introduction: Bacterial meningitis is one of the top ten causes of death amongst children under 5 years in Zimbabwe. Optimizing the identification of the etiologic agents of bacterial meningitis leads to better management of patients. The objective of this study was to compare the effectiveness of latex agglutination (LA), culture and Polymerase Chain Reaction (PCR) as diagnostic methods in the detection of Neisseria meningitidis, Streptococcus pneumoniae, Streptococcus agalactiae and Haemophilus influenzae in paediatric cerebral spinal fluids (CSF) specimens at Harare Children`s Hospital (HCH). \u0000Methodology: Specimens from 162 clinically suspected paediatric cases of bacterial meningitis were processed by cell count, Gram stain, culture, latex agglutination and PCR. \u0000Results: Forty-nine (30.2%) suspected cases were positive for at least one of the four bacterial organisms. The latex agglutination test was positive in 33/49 (67.3%) cases, PCR was positive in 37/49 (75.5%) and culture was positive for 17/49 (34.7%) cases. Streptococcus pneumoniae was the predominant pathogen detected in 29 of the 49 positive cases (59.2%) followed by S. agalactiae detected in 11/49 (22.4%) cases. Haemophilus influenzae was detected in 7/49 (14.3%) cases while N. meningitidis accounted for 2/49 (4.1%) positive cases. Thirty-three (20.4%) CSF samples tested positive with the latex agglutination test. This increased the number of organisms above that detected by culture by 16/49 (32.6%). Polymerase chain reaction detected 37 CSF samples increasing the number of organisms detected by culture by 20/49 (40.8%). \u0000Conclusion: Bacterial meningitis mainly caused by Streptococcus pneumoniae is prevalent among children in Zimbabwe and coupling of culture and non-culture methods can improve detection of the disease.","PeriodicalId":150547,"journal":{"name":"Journal of Cell Science and Therapy","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129838385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

AQP7 Up-Regulation in the Skeletal Muscles of Mice with Diet Induced Obesity 饮食性肥胖小鼠骨骼肌AQP7上调

Journal of Cell Science and Therapy Pub Date : 2015-04-20 DOI: 10.4172/2157-7013.1000205

Y. Wakayama, Satoshi Hirako, T. Jimi, S. Shioda

{"title":"AQP7 Up-Regulation in the Skeletal Muscles of Mice with Diet Induced Obesity","authors":"Y. Wakayama, Satoshi Hirako, T. Jimi, S. Shioda","doi":"10.4172/2157-7013.1000205","DOIUrl":"https://doi.org/10.4172/2157-7013.1000205","url":null,"abstract":"Aquaporin (AQP) 7 and AQP9 are membrane proteins and are the members of aquaglyceroporin which transports glycerol in addition to water molecule. Glycerol is a direct source of glycerol-3 phosphate for the synthesis of triglycerides. We thought that the expression of AQP7 and AQP9 would be altered in the skeletal myofibers in mice with diet-induced obesity (DIO) as compared with that of control chaw-fed mice. RNA and protein levels of AQP7 and AQP9 were studied in the quadriceps femoris muscles of mice with DIO and normal control mice. Real time quantitative RT-PCR analysis showed that mouse AQP7 mRNA levels in skeletal muscles were significantly higher in mice with DIO than in normal control mice (P 0.05). Histochemically the myofibers of mice with DIO contained numerous lipid droplets in oil red O stain samples. Immunohistochemical study of DIO mouse muscles showed enhanced expression of AQP7 at the myofiber surface membranes; while AQP9 expression appeared to be similar to that of normal control mice. These findings imply that the up-regulated expression of AQP7 in DIO mouse muscles facilitates the secretion of glycerol from myocytes.","PeriodicalId":150547,"journal":{"name":"Journal of Cell Science and Therapy","volume":"48 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117271023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Molecular Modeling to Study Protein Function 研究蛋白质功能的分子模型

Journal of Cell Science and Therapy Pub Date : 2015-04-15 DOI: 10.4172/2157-7013.1000E121

A. Morrée

引用次数: 0

Bone marrow cells influences for function of rat decidua 骨髓细胞对大鼠蜕膜功能的影响

Journal of Cell Science and Therapy Pub Date : 2015-04-15 DOI: 10.4172/2157-7013.1000224

V. Mikhailov, A. Domnina, Sokolov Av, Rozanov Jm, Kaminskaya Kv, Nikolsky Nn

{"title":"Bone marrow cells influences for function of rat decidua","authors":"V. Mikhailov, A. Domnina, Sokolov Av, Rozanov Jm, Kaminskaya Kv, Nikolsky Nn","doi":"10.4172/2157-7013.1000224","DOIUrl":"https://doi.org/10.4172/2157-7013.1000224","url":null,"abstract":"Background: In this article we described the influence of transplantation of pregnant rat BMC to pregnant rats of the same date of pregnancy to study the influence on fetus’s development. \u0000Methods: To study we made a single intravenous transplantation of BMC of pregnant rats of 4-5, 7-8 or 11, 12 days to pregnant rats of the same date of pregnancy. The analyses were made at 18th day of pregnancy. \u0000Results: The transplantation of rat BMC at 4, 5 pregnant days increases pre- and post-implantation death of fetuses without change of their weight and weight of placentas. The transplantation of BMC after implantation at 7, 8, 9 days of pregnancy during gastrulation increased the weight of 18th day fetuses and of placentas in comparison with the same parameters of usual normal fetuses. The survival of fetuses was not disturbed. In case of BMC transplantation during placentation at 11, 12 days of pregnancy the weight of fetuses and survival of fetuses was significantly decreased and the weight of placentas was increased. \u0000Conclusions: Results of BMC transplantation depends on the stage of rat pregnancy. The increase of the weight of fetuses after BMC transplantation within gastrulation may be explained by positive paracrine effects of allogenic transplanted cells on the size of decidua and in turn on the growth of fetuses and placentas or by transplanted cells itself. The transplantation of BMC at 11, 12 day of pregnancy during placentation decrease the weight of fetuses at 18th day of pregnancy and increase the weight of placentas. The possibility to regulate a weight of fetuses by allogenic BMC transplantation at gastrulation is important results for stem cell therapy of fetuses.","PeriodicalId":150547,"journal":{"name":"Journal of Cell Science and Therapy","volume":"50 1-2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126939727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Current driving factors in stem cell-based regenerative medicine 当前干细胞再生医学的驱动因素

Journal of Cell Science and Therapy Pub Date : 2015-04-14 DOI: 10.4172/2157-7013.S1.031

J. Sherley

引用次数: 0