Journal of Applied Microbiology最新文献_第8页

Evaluation of the antimicrobial activity of gut catabolites of quercetin 4'-glucoside on Listeria monocytogenes and their effects on membrane integrity and biofilm formation. 槲皮素4′-葡萄糖苷肠道分解代谢物对单核增生李斯特菌的抑菌活性及其对膜完整性和生物膜形成的影响

IF 3.2 3区生物学

Journal of Applied Microbiology Pub Date : 2025-08-04 DOI: 10.1093/jambio/lxaf183

Yujia Liu, Yue Gao, Conghui Shi, Xiaoyang Liu

{"title":"Evaluation of the antimicrobial activity of gut catabolites of quercetin 4'-glucoside on Listeria monocytogenes and their effects on membrane integrity and biofilm formation.","authors":"Yujia Liu, Yue Gao, Conghui Shi, Xiaoyang Liu","doi":"10.1093/jambio/lxaf183","DOIUrl":"10.1093/jambio/lxaf183","url":null,"abstract":"Aims: Listeria monocytogenes is the well-known foodborne pathogen that requires critical control in the human body. 3-hydroxyphenylacetic acid (OPAC) and 3,4-dihydroxyphenylacetic acid (DOPAC) are gut catabolites of quercetin 4'-glucoside in onion, their antibacterial effects would help to control the risk of L. monocytogenes. Here, we investigate their antibacterial activity, action at the transcript level and effects on membrane and biofilm to better control L. monocytogenes.Methods and results: RNA sequencing data revealed that the OPAC and DOPAC treatments to L. monocytogenes led to the identification of enormously differently expressed genes in cellular components (membrane) and phosphotransferase system pathway at their 1/2 MIC (7.805 mM l-1). DOPAC exhibited more obviously damaged in membrane (max 33.94%) and biofilm (max 88.2%) than that of OPAC which may be related to their different effects on cell hydrophobicity, mobility and morphology.Conclusions: These findings provided a credibly inhibitory mechanism of OPAC and DOPAC on L. monocytogenes, offering the theoretical basis for potential applications on the other kinds of phenolic acids that would possess similar structures in food industry.","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144659248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IF 3.2 3区生物学

Journal of Applied Microbiology Pub Date : 2025-08-04 DOI: 10.1093/jambio/lxaf190

Yamima Tasnim, Md Kaisar Rahman, Babafela Awosile

{"title":"Genetic relatedness and antimicrobial resistance profiles of β-lactamase-producing Escherichia coli from environmental and animal sources in West Texas: a One Health approach.","authors":"Yamima Tasnim, Md Kaisar Rahman, Babafela Awosile","doi":"10.1093/jambio/lxaf190","DOIUrl":"10.1093/jambio/lxaf190","url":null,"abstract":"Aims: We aimed to determine the genetic relatedness among β-lactamase-producing Escherichia coli isolated from soil, lake water, and feces of geese, pigs, dogs, cattle, coyotes, wild hogs, and horses at one health interface in West Texas.Methods and results: Previously isolated 56 β-lactamase-producing E. coli isolates from the feces of geese, pigs, horses, coyotes, dogs, cattle, and wild hogs, and soil and lake water from different locations in West Texas were analyzed for genetic relatedness using whole-genome sequencing, core genome multilocus sequence typing, and phylogenetic single-nucleotide polymorphism (SNP) analysis using the publicly available bioinformatic platforms. Among 56 β-lactamase-producing E. coli isolates, 82.1% carried blaCTX-M genes, 42.86% carried blaTEM genes, 7.14% carried blaOXA-10, and 3.57% contained blaCMY-2 genes. Nine different types of phylogroups, 35 serotypes and 36 sequence types, 32 distinct plasmid groups, 75 types of virulence genes, and 46 core genome multilocus sequence types were identified. Using 100 maximum of SNPs between the isolates to define a clone, 14 clones were identified in 13 E. coli isolates from animal feces and environmental samples. The clonally related E. coli isolates were clustered based on the phylogenetic tree.Conclusion: The findings from this study provide limited evidence of clonal dissemination of β-lactamase-producing E. coli between different animal sources.","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144707562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of the survival capacity and transcriptional response of Salmonella enterica serovar Infantis under stress conditions of clinical and food safety relevance. 应激条件下肠沙门氏菌血清型婴儿的生存能力和转录反应分析与临床和食品安全相关。

IF 3.2 3区生物学

Journal of Applied Microbiology Pub Date : 2025-08-04 DOI: 10.1093/jambio/lxaf186

Felipe Pinheiro Vilela, Dália Dos Prazeres Rodrigues, Daniel Beiting, Juliana Pfrimer Falcão

{"title":"Analysis of the survival capacity and transcriptional response of Salmonella enterica serovar Infantis under stress conditions of clinical and food safety relevance.","authors":"Felipe Pinheiro Vilela, Dália Dos Prazeres Rodrigues, Daniel Beiting, Juliana Pfrimer Falcão","doi":"10.1093/jambio/lxaf186","DOIUrl":"10.1093/jambio/lxaf186","url":null,"abstract":"Aims: Analyze the survival and transcriptional response of Salmonella Infantis (S. Infantis) against acid, oxidative, increased osmolarity, and thermal stresses.Methods and results: 25 strains isolated from human, food, and veterinary sources in Brazil between 2013 and 2018 were analyzed. 8 log10 CFU ml-1 inoculums were exposed for 10 min, 1 h, or 24 h to acid stress (HCl pH 2.6); oxidative stress (15 mmol l-1 H2O2); NaCl 9%; refrigeration (4°C); freezing (-20°C); and heating (63/74°C). Most strains (n = 22) survived the acid stress after 10 min and 1 h. Strains maintained high populations under oxidative stress (>87.7%) and NaCl 9% (111.4%). No strains survived heating, while refrigeration prevented multiplication (∼101%), and freezing reduced populations (<92.6%). Strain 1143/14 had its transcriptome sequenced under acid and oxidative stresses and NaCl 9%. 14 up-regulated and 38 down-regulated transcripts under acid stress, and 407 up-regulated and 143 down-regulated under oxidative stress were expressed, associated with biological processes of response and adaptation. No transcripts were differentially expressed with NaCl 9%.Conclusions: Salmonella Infantis survival against most stresses represents a challenge for its control. The efficacy of heating reinforced its importance to control Salmonella in food. While acid survival involved the expression of virulence and broad stress response genes, oxidative stress survival showed the expression of genes specific to the adaptation to this condition.","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144760115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanism of CaCO3 efficiently promoting hydrogen production by Clostridium butyricum BJ-10. CaCO3有效促进丁酸梭菌BJ-10产氢的机理

IF 3.2 3区生物学

Journal of Applied Microbiology Pub Date : 2025-08-04 DOI: 10.1093/jambio/lxaf206

Ting Huang, Rou Zhao, Yuanshan Lin, Lingzhi Zhu, Qiaoyi Zhang, Jing Qin, Lin Zhu, Yun Tian, Huhu Liu, Chong Wang, Guiping Guan

{"title":"Mechanism of CaCO3 efficiently promoting hydrogen production by Clostridium butyricum BJ-10.","authors":"Ting Huang, Rou Zhao, Yuanshan Lin, Lingzhi Zhu, Qiaoyi Zhang, Jing Qin, Lin Zhu, Yun Tian, Huhu Liu, Chong Wang, Guiping Guan","doi":"10.1093/jambio/lxaf206","DOIUrl":"10.1093/jambio/lxaf206","url":null,"abstract":"Aims: This study aims to explore the effect of adding CaCO3 on the hydrogen production by the fermentation of Clostridium butyricum (C. butyricum) BJ-10 and the mechanism of promoting hydrogen production by C. butyricum.Methods and results: Determine the effect of CaCO3 on hydrogen production by C. butyricum through fermentation. When the concentration of CaCO3 was 50 mmol·L-1, the cumulative hydrogen production was 3726.27 mL·L-1, which was 193% higher than that of the control group. By exploring the effects of adding CaCl2, Na2CO3, and pH control on the hydrogen production of C. butyricum, it was found that the main reason why CaCO3 promoted the hydrogen production of C. butyricum was that it could control the pH of the fermentation broth within a suitable range. Transcriptomic analyses were performed on C. butyricum with and without the addition of CaCO3, respectively. After the addition of CaCO3, a total of 222 genes were significantly expressed, of which 141 genes were significantly upregulated and 81 genes were significantly downregulated. Among them, genes related to carbohydrate metabolism were upregulated (pgm by 1.95-fold, eno by 2.33-fold, and pflD by 3.43-fold).Conclusions: CaCO3 can accelerate the process of glycolysis by upregulating the pgm and eno genes, and further promote the increase in hydrogen production by upregulating the pflD gene. This study can provide a reference for the development of biological hydrogen production technology.","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144855239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An investigation of the microbiological quality of smoked fish in England and detection of Listeria monocytogenes strains associated with listeriosis. 英国熏鱼微生物质量调查及与李斯特菌病相关的单核细胞增生李斯特菌菌株检测。

IF 3.2 3区生物学

Journal of Applied Microbiology Pub Date : 2025-08-04 DOI: 10.1093/jambio/lxaf192

Frieda Jorgensen, Michelle Kesby, Corinne Amar, Dunstan Rajendram, Clare Barker, Adam Crewdson, Anais Painset, Lorraine Sadler-Reeves, Heather Aird, Sandra Lai, Caroline Willis

{"title":"An investigation of the microbiological quality of smoked fish in England and detection of Listeria monocytogenes strains associated with listeriosis.","authors":"Frieda Jorgensen, Michelle Kesby, Corinne Amar, Dunstan Rajendram, Clare Barker, Adam Crewdson, Anais Painset, Lorraine Sadler-Reeves, Heather Aird, Sandra Lai, Caroline Willis","doi":"10.1093/jambio/lxaf192","DOIUrl":"10.1093/jambio/lxaf192","url":null,"abstract":"Aims: A survey to determine the presence of Listeria and indicator bacteria in smoked fish was performed from 2022 to 2023.Methods and results: Listeria monocytogenes was detected in 3.6% of 783 samples. Listeria monocytogenes was detected more frequently in cold smoked (4.9%) compared to hot smoked (2.0%) samples. The majority (63%) of samples had a water activity that would not prevent the growth of L. monocytogenes. Elevated levels of Enterobacteriaceae (>10 000 CFU g-1) were detected in 4.7% of samples. Listeria monocytogenes was detected more frequently in samples with elevated levels of Enterobacteriaceae. Isolates of L. monocytogenes were compared using whole genome sequencing to historic isolates. Isolates from three samples were the same strain as that associated with a clonal complex (CC) 217 outbreak under investigation and detected despite an earlier product recall. Twelve isolates were CC121 strains and 10 of these belonged to six single nucleotide polymorphism single-linkage clusters with one strain linked to a human case from 2021. One CC415 strain was also linked to a human case. The remaining isolates belonged to CC8, CC9, CC14, CC37, CC31, CC398, CC87, and CC475 with no links to human cases. Investigation of historical isolates revealed that 11 strains were associated with specific manufacturers for up to 10 years. The presence of quaternary ammonium compound efflux genes showed an association with CC.Conclusions: Diverse L. monocytogenes detected in smoked fish with persistent strains associated with some manufacturing premises. Correlation between presence of L. monocytogenes and high levels of Enterobacteriaceae was detected.","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144731100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic analyses of Escherichia coli ST410 harbouring the blaOXA-181 carbapenemase gene from companion animals and humans in Switzerland. 瑞士伴侣动物和人类携带blaOXA-181碳青霉烯酶基因的大肠杆菌ST410基因组分析

IF 3.2 3区生物学

Journal of Applied Microbiology Pub Date : 2025-08-04 DOI: 10.1093/jambio/lxaf181

Yvonne Spahr, Patrice Nordmann, Javier E Fernandez, Laurent Poirel, Andrea Endimiani, Vincent Perreten

{"title":"Genomic analyses of Escherichia coli ST410 harbouring the blaOXA-181 carbapenemase gene from companion animals and humans in Switzerland.","authors":"Yvonne Spahr, Patrice Nordmann, Javier E Fernandez, Laurent Poirel, Andrea Endimiani, Vincent Perreten","doi":"10.1093/jambio/lxaf181","DOIUrl":"10.1093/jambio/lxaf181","url":null,"abstract":"Aims: To determine the genomic relatedness of E. coli (Ec) sequence type (ST) 410 producing the carbapenemase OXA-181 (ST410-OXA-181), which caused an outbreak in a Swiss companion animal (CA) clinic in 2018 with those isolated from humans between 2017 and 2021 in Switzerland, and to characterize complete plasmids harbouring antimicrobial resistance genes (ARGs).Methods and results: The complete genomes of 31 Ec ST410-OXA-181 (8 from CA, 23 from humans) were obtained by hybrid assembly of reads from Illumina and Nanopore sequencing technologies. The genomes were used for in silico phylogenetic analysis (cgMLST, SNP, ST410 clade analysis), ARG screening and for comparative analysis of complete plasmids. Antimicrobial susceptibility was determined by MIC measurement. All veterinary strains (CA, veterinarian) belonged to the phylogenetic clade ST410-B2 and the human strains to either ST410-B2 or B3. Strains recovered from the veterinary setting were clonal, differing by 0-4 SNPs. A higher genetic distance (>131 SNPs) was observed between the veterinary and the clinical human strains. All veterinary and 15 human strains shared an identical IncX3 plasmid harbouring blaOXA-181. Additional multidrug resistance plasmids were detected in human strains only.Conclusions: The Ec ST410-B2-OXA-181 lineage was detected in both humans and animals. Strains share the same OXA-181 plasmids, but differed by SNP-based genetic distances and ARG contents indicating broad dissemination potential and independent evolution of strains of this lineage.","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144731102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sanguinarine hydrogel accelerates wound healing in Staphylococcus pseudintermedius infections by suppressing biofilm formation. 血根碱水凝胶通过抑制生物膜的形成加速假中葡萄球菌感染的伤口愈合。

IF 3.2 3区生物学

Journal of Applied Microbiology Pub Date : 2025-08-04 DOI: 10.1093/jambio/lxaf184

Jintong Liu, Jiahui Wu, Ting Zheng, Di Zhang, Jiahao Lin

{"title":"Sanguinarine hydrogel accelerates wound healing in Staphylococcus pseudintermedius infections by suppressing biofilm formation.","authors":"Jintong Liu, Jiahui Wu, Ting Zheng, Di Zhang, Jiahao Lin","doi":"10.1093/jambio/lxaf184","DOIUrl":"10.1093/jambio/lxaf184","url":null,"abstract":"Aims: Skin and ear infections are common in small animals, primarily caused by the opportunistic pathogen Staphylococcus pseudintermedius. Staphylococcus pseudintermedius biofilms are associated with poor prognosis, and rising antibiotic resistance threatens public health, making the development of new antibacterial agents imperative. Sanguinarine (SAN) is a plant-derived alkaloid with a wide range of pharmacological activities, including antibacterial, anti-inflammatory, and antitumour effects. The antibacterial potential of SAN against S. pseudintermedius merits further investigation.Methods and results: The minimum inhibitory concentration and minimum bactericidal concentration of SAN hydrogel against clinically isolated S. pseudintermedius were determined as 1.25 and 5 mg mL-1, respectively. Phenol-sulfuric acid and XTT assays demonstrated that SAN hydrogel significantly delayed biofilm formation (P < 0.05) by suppressing exopolysaccharide synthesis and biofilm metabolic activity. Live/dead fluorescent staining and scanning electron microscopy (SEM) confirmed its potent disruptive effects on mature biofilms, inducing bacterial lysis and death, while reducing biofilm thickness and extracellular matrix. In a murine skin infection model, SAN hydrogel (2.5/5 mg mL-1) exhibited significant antibacterial efficacy (P < 0.001), accelerated wound healing, and reduced bacterial load (P < 0.001) and IL-6 levels (P < 0.0001) in skin tissues. No significant hepatorenal toxicity was observed within the tested concentration range.Conclusion: The SAN hydrogel demonstrates antimicrobial and bactericidal activities against S. pseudintermedius, effectively suppressing biofilm formation and holding promise as a therapeutic candidate for skin infections caused by this pathogen.","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144674881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Natural products eugenol and cinnamaldehyde as potential therapeutic alternatives against Candida auris infections. 天然产物丁香酚和肉桂醛作为抗耳念珠菌感染的潜在治疗选择。

IF 3.2 3区生物学

Journal of Applied Microbiology Pub Date : 2025-08-04 DOI: 10.1093/jambio/lxaf197

Débora S M de Sousa, Gabrielle P M Monari, Cassiana F da Rosa, Jacques F Meis, Ana Flavia M Pereira, Tatiane B Zapata, Cauê B T Dos Santos, Mirian C E Aal, Flavia K Delella, Sandra M G Bosco, Ary F Júnior, Márcia S C Melhem, Eloisa F Clemente, Bruna B C Mendonça, Bruno C Rossini, Lucilene D Dos Santos, Luciana S Ruiz

{"title":"Natural products eugenol and cinnamaldehyde as potential therapeutic alternatives against Candida auris infections.","authors":"Débora S M de Sousa, Gabrielle P M Monari, Cassiana F da Rosa, Jacques F Meis, Ana Flavia M Pereira, Tatiane B Zapata, Cauê B T Dos Santos, Mirian C E Aal, Flavia K Delella, Sandra M G Bosco, Ary F Júnior, Márcia S C Melhem, Eloisa F Clemente, Bruna B C Mendonça, Bruno C Rossini, Lucilene D Dos Santos, Luciana S Ruiz","doi":"10.1093/jambio/lxaf197","DOIUrl":"https://doi.org/10.1093/jambio/lxaf197","url":null,"abstract":"Aims: Candida auris has been reported on all continents, becoming a global threat due to high levels of resistance to all antifungal classes. In addition, this pathogen has lower susceptibility to disinfectants used in hospitals, favoring its stay in such environments. Evaluate the action of eugenol and cinnamaldehyde against Candida auris.Methods and results: The products were evaluated through sensitivity tests of broth microdilution, as well as their possible mechanisms of action using proteomic analysis. For microdilution was used 24 strains and for synergistic interactions was used 2 strains. Cytotoxicity of these products was verified in Vero cells. Eugenol and cinnamaldehyde showed fungicidal action at concentrations ranging from 31.25 to 250 µg/mL. Combinations of eugenol and cinnamaldehyde with itraconazole showed either antagonistic effects or no detectable interaction. No cytotoxicity was found for eugenol at the concentrations that caused yeast growth inhibition. On the other hand, cinnamaldehyde was cytotoxic at the same concentrations. According to proteomic analysis, the mechanism of action of eugenol and cinnamaldehyde is possibly related to the biosynthesis of ergosterol.Conclusion: The present results suggest eugenol as a possible candidate for the development of formulations to treat infections caused by C. auris.","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":"136 8","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144955162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extracellular vesicles from Fusarium oxysporum can induce a cytotoxic response in an invertebrate model. 在无脊椎动物模型中，尖孢镰刀菌的细胞外囊泡可以诱导细胞毒性反应。

IF 3.2 3区生物学

Journal of Applied Microbiology Pub Date : 2025-08-04 DOI: 10.1093/jambio/lxaf194

Deisiany Gomes Ferreira, Bruna Sabatke, Izadora Volpato Rossi, Jhon J Artunduaga Bonilla, Leandro Honorato, Abel Sana, Leticia Bassani Bonato, Leonardo Nimrichter, Marcel Ivan Ramirez, Melyssa Negri

{"title":"Extracellular vesicles from Fusarium oxysporum can induce a cytotoxic response in an invertebrate model.","authors":"Deisiany Gomes Ferreira, Bruna Sabatke, Izadora Volpato Rossi, Jhon J Artunduaga Bonilla, Leandro Honorato, Abel Sana, Leticia Bassani Bonato, Leonardo Nimrichter, Marcel Ivan Ramirez, Melyssa Negri","doi":"10.1093/jambio/lxaf194","DOIUrl":"10.1093/jambio/lxaf194","url":null,"abstract":"Aims: One of the ways fungi communicate is through extracellular vesicles (EVs), which vary in size and content depending on the species and fungal form. This study investigated the release of EVs from Fusarium oxysporum, noting significant differences in their structure and biological effects in both in vitro and in vivo.Methods and results: The study found that the size of these vesicles was consistent with patterns observed in other fungal species. Notably, the release of EVs from the planktonic form increased exponentially after 120 h of growth, resulting in heightened vesicle production. In biofilm, EVs released from the biofilm matrix showed distinct characteristics from those secreted externally, with a peak in release at 24 h. The concentration of EVs inside the biofilm was significantly higher, reflecting the complexity of biofilm dynamics. Additionally, EVs from the biofilm, particularly those isolated at 48 h of maturation, were highly cytotoxic, causing over 95% cell death in human cells. In vivo experiments with Tenebrio molitor larvae showed that EVs from biofilms could induce significant mortality.Conclusion: These findings suggest that EVs may serve as communicators and can also trigger pathogenic effects by transporting cytotoxic molecules.","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144742131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of the G protein-coupled receptor FfGpr1-Gα-AC signal transduction in Fusarium fujikuroi and its role in gibberellin biosynthesis. 藤黑镰刀菌GPCR - FfGpr1-Gα-AC信号转导及其在赤霉素合成中的作用

IF 3.2 3区生物学

Journal of Applied Microbiology Pub Date : 2025-08-04 DOI: 10.1093/jambio/lxaf191

Yu-Ke Cen, Ming-Han Li, Jiang-Tao Li, Jing-Wen Jia, Qi Wang, Xu-Sheng Liu, Yuan-Shan Wang, Ya-Ping Xue, Zhi-Qiang Liu, Yu-Guo Zheng

{"title":"Characterization of the G protein-coupled receptor FfGpr1-Gα-AC signal transduction in Fusarium fujikuroi and its role in gibberellin biosynthesis.","authors":"Yu-Ke Cen, Ming-Han Li, Jiang-Tao Li, Jing-Wen Jia, Qi Wang, Xu-Sheng Liu, Yuan-Shan Wang, Ya-Ping Xue, Zhi-Qiang Liu, Yu-Guo Zheng","doi":"10.1093/jambio/lxaf191","DOIUrl":"10.1093/jambio/lxaf191","url":null,"abstract":"Aims: This study aimed to characterize the G protein-coupled receptor FfGpr1-Gα-AC transduction pathway and its role in regulating gibberellin (GA) metabolism in Fusarium fujikuroi.Methods and results: By constructing Ffgpr1 deletion and constitutively activated FfG2Q204 L mutants, we found that glucose-induced cAMP (cyclic Adenosine MonoPhosphate) synthesis was abolished in Ffgpr1Δ. Bimolecular fluorescence complementation confirmed membrane-localized FfGpr1-FfG2 and FfG2-AC interactions, with intensified fluorescence at septa. Fermentation assays revealed opposing GA3 yields: Ffgpr1Δ produced 21% less GA3 than the wild type, whereas FfG2Q204 L increased yield by 17%. qPCR（quantitative real-time PCR） analysis demonstrated that Ffgpr1Δ upregulated FfCPS/KS, FfP450-2, and FfP450-3 transcription by 6-8-fold while downregulating FfDES by 82%, whereas FfG2Q204 L induced a 6-fold increase in FfCPS/KS mRNA level. Strikingly, FfDES overexpression in Ffgpr1Δ restored GA3 production to wild-type levels but led to GA7 accumulation and suppressed FfP450-3 upregulation, suggesting feedback-regulated metabolic constraints.Conclusion: Glucose-induced cAMP production required FfGpr1. FfGpr1-FfG2 and FfG2-AC interacted on the cell membrane, with enhanced co-localization at the septal region. The FfGpr1-Gα-AC pathway significantly affected GA yield, with complex and noteworthy regulation of GA cluster gene expression.","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144731101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0