Geromy G Moore, Matthew D Lebar, Rebecca R Sweany, Jessica M Lohmar, Carol H Carter-Wientjes
{"title":"Production of inhibitory extrolites is a shared trait among non-aflatoxigenic Aspergillus flavus.","authors":"Geromy G Moore, Matthew D Lebar, Rebecca R Sweany, Jessica M Lohmar, Carol H Carter-Wientjes","doi":"10.1093/jambio/lxaf084","DOIUrl":"10.1093/jambio/lxaf084","url":null,"abstract":"<p><strong>Aims: </strong>Assess the potential for Aspergillus flavus biocontrol isolates from different geographic regions to secrete metabolites with inhibitory properties against mycotoxigenic isolates.</p><p><strong>Methods and results: </strong>Using four Aspergillus isolates from Arizona, Georgia, and Mississippi, mycotoxin producing isolates from each region were grown on media previously colonized by a biocontrol isolate to assess the inhibition potential of biocontrol-produced extrolites. Significant changes in growth and/or mycotoxin production were observed.</p><p><strong>Conclusions: </strong>Production of inhibitory extrolites is a trait shared by biocontrol isolates. Mycotoxin reduction was not directly proportional to growth for most isolates, since extrolites secreted by the examined biocontrol isolates were able to greatly reduce mycotoxin production even if growth was minimally inhibited. Only the Georgia isolates exhibited a linear relationship between reduced growth and mycotoxin reductions, indicating that their mycotoxin reductions were primarily due to reduced growth.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143788453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Josh Milmine, Lauren Fletcher, Kai-Hsiang Chang, Younghoon Kang, Minhua Sun, Jessica Churcher, Linoy Jacobs, Yashu Song, Rob Patterson, Elijah G Kiarie, Julang Li
{"title":"Influence of Bacillus licheniformis B4 fermentation on soybean meal nutritional value and early weaned pig growth performance.","authors":"Josh Milmine, Lauren Fletcher, Kai-Hsiang Chang, Younghoon Kang, Minhua Sun, Jessica Churcher, Linoy Jacobs, Yashu Song, Rob Patterson, Elijah G Kiarie, Julang Li","doi":"10.1093/jambio/lxaf103","DOIUrl":"https://doi.org/10.1093/jambio/lxaf103","url":null,"abstract":"<p><strong>Aim: </strong>This study aimed to examine B4's fermentation efficiency and the growth performance of newly weaned pigs fed B4 fermented soybean meal (B4-FSBM).</p><p><strong>Method and results: </strong>Soybean meal (SBM) was inoculated with B4 and fermented at room temperature for 48 hours. Following fermentation, analysis was performed to examine anti-nutritional factor degradation efficiency. Fermentation broke down large molecular weight proteins, consistent with sizes of glycinin and β-conglycinin into smaller proteins. Crude protein significantly increased from 51.5% to 56.5%. Neutral detergent fiber (NDF) was reduced by 26.9% (P < 0.05), while the phytate phosphorus content was reduced by 59.16% (P < 0.05) in B4-FSBM. In the animal trial, 90 newly weaned piglets were divided into three groups, receiving either the negative control (NC; 25.4% SBM), positive control (PC; 25.4% SBM supplemented with 3000 mg/kg zinc oxide), or B4 (B4; 19% FSBM, 6.4% SBM). There was a temporary setback in growth performance for pigs fed B4-FSBM during the early weeks. However, their growth performance improved, and by the fourth week, their gain-to-feed ratio was significantly improved when compared to the control groups. Additionally, pigs fed B4-FSBM had increased (P < 0.05) crude protein digestibility compared to the NC for weeks 3 and 4.</p><p><strong>Conclusions: </strong>Fermentation of SBM with B4 improves feed efficiency and protein digestibility in weaned pigs.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":"136 5","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144063847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Multi-algicidal mechanism and potential application of Streptomyces sp. strain P-10 against the bloom-forming Prorocentrum donghaiense.","authors":"Chenyu Yang, Wei Xiang, Wei Lun Ng, Jing Zhao, Xin Lin","doi":"10.1093/jambio/lxaf109","DOIUrl":"https://doi.org/10.1093/jambio/lxaf109","url":null,"abstract":"<p><strong>Aims: </strong>This study sought to assess the algicidal efficacy of Streptomyces sp. P-10 against Prorocentrum donghaiense and evaluate its real-world application potential.</p><p><strong>Methods and results: </strong>Streptomyces sp. P-10's supernatant and mycelial pellets demonstrated potent algicidal effects against P. donghaiense, with 95% and 80% lysis rates within 96 h, respectively. The supernatant contained N-acetyltryptamine, which at an IC50 of 37.88 μg·L-1, significantly inhibited algal photosynthesis. It also exhibited cellulase activity as 201.37 μg· (mL·min)-1, degrading algal cell walls. Both N-acetyltryptamine and cellulase, alone or in concert, hindered photosynthesis. SEM and TEM analyses revealed that P-10 mycelia enwrapped and lysed algal cells, while supernatant components compromised cell wall integrity and induced cytoplasmic vacuolation. Notably, P-10 sustained growth in algal cultures and bloom waters without additional nutrients.</p><p><strong>Conclusions: </strong>Streptomyces sp. P-10 effectively kills P. donghaiense via direct mycelial encasement and indirect bioactive compounds and cellulase activity, inhibiting algal growth and photosynthesis.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":"136 5","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144063856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mariana Carvalho Sturaro, Rafael Araújo, Larissa Sobrinho Aniceto, Gabrielli Rodrigues de Medeiros, Gleyce Hellen de Almeida de Souza, Simone Simionatto
{"title":"Cephalosporin-based combination therapies for combating ESKAPE pathogens: a patent review.","authors":"Mariana Carvalho Sturaro, Rafael Araújo, Larissa Sobrinho Aniceto, Gabrielli Rodrigues de Medeiros, Gleyce Hellen de Almeida de Souza, Simone Simionatto","doi":"10.1093/jambio/lxaf107","DOIUrl":"10.1093/jambio/lxaf107","url":null,"abstract":"<p><p>ESKAPE (namely Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter) pathogens pose a major threat to global health. The World Health Organization highlights the need for new antimicrobial strategies, including combination therapies, to address their resistance. Cephalosporins, due to their broad-spectrum activity and safety profile, are widely used in hospitals and serve as strong candidates for such regimens. While many studies explore cephalosporin combinations, there is a lack of systematic reviews focused on patent literature. This study analyses patent filings related to cephalosporin-based combination therapies targeting ESKAPE pathogens. The Espacenet database was thoroughly searched using the keywords \"combination,\" \"antibiotics,\" and \"cephalosporin\" yielding 666 patent applications filed up to June 2024. Based on the inclusion criteria, 30 patents were selected for further analysis. Notably, most patents were filed in China and the USA, accounting for up to 30% and 17%, respectively. Most patents were filed by private companies and classified under the International Patent Classification code A61K, indicating their pharmaceutical applications. Additionally, in 2022, the highest number of patents were filed in this area. However, clinical data were included in only two patents, reflecting a broader challenge: the high cost of development limits real-world validation of these combinations. Despite this, the patent landscape offers valuable insights into emerging strategies for combating ESKAPE pathogens with cephalosporin-based therapies.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":"136 5","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144017981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abid Bashir, Showkat Ahmad Bhat, Malik Muzafar Manzoor, Farha Bhatti, Khursheed Ahmad Bhat, Syed Riyaz-Ul-Hassan
{"title":"Secalonic acid F1 derived from an endophytic fungus Periconia verrucosa as a potential antimicrobial agent against Staphylococcus aureus.","authors":"Abid Bashir, Showkat Ahmad Bhat, Malik Muzafar Manzoor, Farha Bhatti, Khursheed Ahmad Bhat, Syed Riyaz-Ul-Hassan","doi":"10.1093/jambio/lxaf120","DOIUrl":"10.1093/jambio/lxaf120","url":null,"abstract":"<p><strong>Aim: </strong>To investigate the antimicrobial potential of a secalonic acid F1 derivative produced by an endophytic Periconia verrucosa.</p><p><strong>Methods and results: </strong>The endophyte RDE85 was characterized as P. verrucosa by morphological and phylogenetic analysis. We characterized a major compound from RDE85 as Secalonic acid F1 (SF1) with a 2,4'-linkage. SF1 demonstrated antimicrobial activity with an IC50 of 7.6 µg mL-1 against Staphylococcus aureus. It inhibited the biofilm formation, causing morphological changes and disruption of cell membrane integrity in the pathogen, confirmed by scanning electron microscopy (SEM). The compound depicted strong synergistic potential with ciprofloxacin and reduced DNA and RNA synthesis. The time-kill kinetics demonstrate that SF1 is an effective concentration-dependent bactericidal agent. Further, SF1 severely affected the respiratory chain dehydrogenase activity, confirmed by in-silico studies, revealing its interaction with respiratory chain succinate dehydrogenase. The treatment with this compound downregulated the staphylococcal accessory gene regulator and enterotoxin gene, two important virulence factors of the organism, and reduced the staphyloxanthin production, which is also an important virulence trait.</p><p><strong>Conclusion: </strong>SF1 is a potential antimicrobial agent against S. aureus.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Anteneh Argaw Alemneh, Gregory R Cawthray, Yi Zhou, Maarten H Ryder, Matthew D Denton
{"title":"A new isolation methodology for phosphate-solubilizing bacteria using a step-wise enrichment process.","authors":"Anteneh Argaw Alemneh, Gregory R Cawthray, Yi Zhou, Maarten H Ryder, Matthew D Denton","doi":"10.1093/jambio/lxaf101","DOIUrl":"10.1093/jambio/lxaf101","url":null,"abstract":"<p><strong>Aims: </strong>Phosphate-solubilizing bacteria (PSB) screened for their ability to solubilize Ca3(PO4)2 in a laboratory may not be effective in solubilizing phosphorus (P) in soils that contain different forms of P. We developed an efficient isolation method to obtain PSB from soils collected from diverse soil types that vary in P forms.</p><p><strong>Methods and results: </strong>PSB isolated through enrichment in media containing AlO4P, Ca3(PO4)2, or FeO4P as sole P sources were compared with a previous collection of Pseudomonas- and Bacillus-like bacteria that solubilized P. There was a significant positive association (P < .05) between the number of culturable PSB and several properties from the original soils, including calcium, magnesium, potassium, total N, P, carbon concentrations, electrical conductivity, and silt content. The number of culturable PSB was inversely related to the sand content of the soil. Additionally, the most efficient PSB were obtained from sites with a high aridity index and from alkaline soils. After enrichment, there was a significant increase in PSB among all culturable bacteria. Isolates obtained after step-wise enrichment solubilized 1.8-2.2, 1.4-2.9, and 2.2-3.3 times more P from Ca3(PO4)2, AlO4P, and FeO4P, respectively, compared with strains selected using a taxonomic method. Isolates enriched using FeO4P produced predominantly tri- and dicarboxylates in vitro. Additionally, we identified an efficient P solubilizer that is closely related to Pseudomonas granadensis, a species not previously reported as a P solubilizer.</p><p><strong>Conclusion: </strong>Using a step-wise enrichment method in culture media with sparingly soluble P increased the likelihood of isolating high-efficiency PSB from soil.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144101911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Correction to: A single upstream mutation of whiB7 underlies amikacin and clarithromycin resistance in Mycobacterium abscessus.","authors":"","doi":"10.1093/jambio/lxaf106","DOIUrl":"https://doi.org/10.1093/jambio/lxaf106","url":null,"abstract":"","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":"136 5","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144005243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Therapeutic effect of Faecalibacterium longum CM04-06 on DSS-induced ulcerative colitis in mice.","authors":"Xiaofang Li, Ningning He, Haoyu Wang, Zhinan Wu, Mengmeng Wang, Hewei Liang, Liang Xiao, Zizhen Yang, Cunyin Li, Ping Xu, Tong Dai, Shangyong Li, Yuanqiang Zou","doi":"10.1093/jambio/lxaf119","DOIUrl":"10.1093/jambio/lxaf119","url":null,"abstract":"<p><strong>Aims: </strong>This study explores the impact of Faecalibacterium longum CM04-06 on inflammatory bowel disease (IBD) by regulating gut microbiota in mice.</p><p><strong>Methods and results: </strong>We reanalyzed the distribution of the CM04-06 genome in the metagenome of the IBD cohort and observed a significantly higher abundance of CM04-06 in healthy individuals compared to patients with UC or CD. The prophylactic administration of CM04-06 was evaluated for its effects on intestinal microbial diversity and community composition after a two-week trial in mice. The intestinal microbiota was characterized using metagenomic sequencing of fecal samples on the DNBSEQ platform. CM04-06 treatment resulted in a significant reduction in the Disease Activity Index (DAI) and histological scores, as well as a decrease in the levels of pro-inflammatory cytokines, including IL-1β, IL-6, and TNF-α, in both the colon and serum of DSS-induced mice. Furthermore, supplementation with CM04-06 significantly reduced the levels of pro-inflammatory cytokines in both the colon and serum. Additionally, CM04-06 enhanced the integrity of the intestinal epithelial barrier by increasing the expression of tight junction proteins and mucin. Moreover, we observed greater abundances of Faecalibaculum rodentium, Alistipes onderdonkii, Alistipes shahii, and Bifidobacterium animalis after CM04-06 treatment.</p><p><strong>Conclusions: </strong>CM04-06 prevents and alleviates intestinal inflammation by modulating the composition of the microbiota community, increasing the abundance of beneficial probiotics, and suppressing pro-inflammatory cytokine levels.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Vivian Ribeiro Pimentel, Fabiano Silva Soares, Leandro Fernandes Andrade, Luciano de Souza Vespoli, Mariana Ramos Leandro, Júlia Rosa Moreira, Vanildo Silveira, Gonçalo Apolinário de Souza Filho
{"title":"Cobalt-induced stress reveals a prominent role of CzcC on the proteomic profile of Gluconacetobacter diazotrophicus PAL5.","authors":"Vivian Ribeiro Pimentel, Fabiano Silva Soares, Leandro Fernandes Andrade, Luciano de Souza Vespoli, Mariana Ramos Leandro, Júlia Rosa Moreira, Vanildo Silveira, Gonçalo Apolinário de Souza Filho","doi":"10.1093/jambio/lxaf113","DOIUrl":"10.1093/jambio/lxaf113","url":null,"abstract":"<p><strong>Aims: </strong>Heavy metal accumulation in agricultural areas is a global environmental problem that affects microorganisms and plants, with serious implications for human health. This study aimed to investigate the molecular responses of the plant growth-promoting bacterium Gluconacetobacter diazotrophicus PAL5 to cobalt stress.</p><p><strong>Methods and results: </strong>We evaluated bacterial growth and cell viability under cobalt stress and performed comparative proteomic and reverse genetics analyses. Cobalt significantly inhibited bacterial growth but did not cause cell death. Proteomic analysis in the presence of 2.5 mmol l-1 CoCl2, which caused ∼50% growth inhibition, revealed the induction of pathways related to iron uptake, carbohydrate metabolism, amino acid metabolism, quality control, and efflux. Knockout mutants for genes involved in these pathways (∆tbdR, ∆zwf, ∆pdhB, ∆argH, and ∆czcC) confirmed the essential role of the CzcC efflux system in cobalt tolerance.</p><p><strong>Conclusions: </strong>Cobalt stress triggers molecular responses in G. diazotrophicus PAL5, with efflux systems playing a crucial role in stress tolerance.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":"136 5","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144127718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nwamaka Oluchukwu Akpodiete, Bianca Carlos, Kamila Voges, Bruno Tinoco Nunes, Jayme Augustus Souza-Neto, Florian Noulin, Daniel Tonge, Wan Fatma Zuharah, Frédéric Tripet
{"title":"Improvement of water quality for mass anopheline rearing: dynamics of larval tray bacterial communities under different water treatments revealed by 16S ribosomal RNA amplicon sequencing.","authors":"Nwamaka Oluchukwu Akpodiete, Bianca Carlos, Kamila Voges, Bruno Tinoco Nunes, Jayme Augustus Souza-Neto, Florian Noulin, Daniel Tonge, Wan Fatma Zuharah, Frédéric Tripet","doi":"10.1093/jambio/lxaf110","DOIUrl":"https://doi.org/10.1093/jambio/lxaf110","url":null,"abstract":"<p><strong>Background: </strong>Immature anophelines inhabit aquatic environments with diverse physicochemical properties and microorganisms. In insectary settings, ammonia accumulation in larval rearing trays can lead to high larval mortality. Bacterial communities in these trays may influence ammonia levels through nitrification and denitrification. While symbiotic bacteria are known to be crucial for nutrition, digestion, reproduction, and immune responses in anophelines, the microbial communities specifically associated with Anopheles coluzzii larvae have not been characterised.</p><p><strong>Methods and results: </strong>Building on a study examining ammonia-capturing zeolite and water changes for rearing Anopheles coluzzii, this research characterised the bacterial communities using 16S rRNA gene sequencing to identify species linked to larval survival and phenotypic quality. Functional filters were applied to identify bacteria related to ammonia nitrification and their impact on larval development. qPCR was used to validate the sequencing data for the 10 most significant bacteria. Water changes significantly reduced bacterial diversity and abundance, improving adult mosquito development and quality. In contrast, untreated trays showed a higher abundance of potentially harmful bacteria, adversely affecting development. Applying zeolite increased nitrifying bacteria presence, benefiting mosquito growth while lowering toxic bacteria levels-trends confirmed by qPCR.</p><p><strong>Conclusions: </strong>This study offers insights into the bacterial communities in mosquito larval-rearing water, highlighting species that could enhance ammonia nitrification and overall rearing success.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":"136 5","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143991223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}