Journal of Applied Microbiology最新文献

{"title":"Salmonella-specific phages from captive bearded dragons (Pogona vitticeps): temperate traits and temperature-dependent variability in inhibition across isolates.","authors":"Kim Küper, Sophie Kittler, Elisa Peh, Juliane Hirnet, Johannes Hetterich, Johanna Sophia Kopp, Madeleine Plötz, Karl Rohn, Mathias Müsken, Angelika Fruth, Michael Pees","doi":"10.1093/jambio/lxag188","DOIUrl":"10.1093/jambio/lxag188","url":null,"abstract":"<p><strong>Aims: </strong>Zoonotic infections with Salmonella spp. transmitted from reptiles to humans are an increasing concern due to the growing number of documented cases and the close contact between humans and reptiles. Reptiles, such as bearded dragons (Pogona vitticeps), frequently carry Salmonella enterica asymptomatically as part of their intestinal microbiota.Given the rise of antibiotic-resistant Salmonella strains in reptiles, bacteriophages (phages) may provide a targeted and sustainable alternative for preventing reptile-to-human transmission.</p><p><strong>Methods and results: </strong>Seventeen phages were isolated from ten of eighteen fecal samples collected from bearded dragons. Seven of these phages were selected for further analyses. Host range assays on 41 S. enterica and nine non-Salmonella isolates revealed a narrow spectrum: phages infected up to 63.4% of Salmonella isolates and lysed one non-Salmonella strain. Planktonic killing assays at 25 and 37°C showed pronounced bacterial growth reduction, with 8 of 12 significant inhibitions observed at 37°C. Phage cocktails generally showed stronger inhibition than individual phages. Electron microscopy and whole-genome sequencing identified six Myovirus-like and one Siphovirus-like phage, all temperate with integrase or transposase genes despite lytic activity.</p><p><strong>Conclusions: </strong>This study expands knowledge of S. enterica-specific phages from reptiles, detailing host specificity, morphology, and genomic features. While in vitro results are promising, in vivo efficacy may be influenced by host physiology, immunity, and microbiome interactions. The predominance of temperate phages may limit direct therapeutic use, though low lysogeny rates in related phages and genetic engineering advances may enable future applications.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148578681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Klebsiella species isolated from diseased dogs in Brazil reveal multidrug resistance, CTX-M-15 predominance and detection of a KPC-2-producing ST11 Klebsiella pneumoniae isolate.","authors":"Patrik Júnior de Lima Paz, Thaís Spessotto Bello, Marcelo Fagali Arabe-Filho, Fábio Vinícius Ramos Portilho, Emanoelli Aparecida Rodrigues Dos Santos, Carlos Henrique Camargo, Vera Lúcia Móres Rall, Juliano Gonçalves Pereira, Rogério Giuffrida, Márcio Garcia Ribeiro","doi":"10.1093/jambio/lxag198","DOIUrl":"10.1093/jambio/lxag198","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the antimicrobial resistance, β-lactamase genes, biofilm formation, and genomic characteristics of Klebsiella species isolated from diseased dogs in southeastern Brazil.</p><p><strong>Methods: </strong>Fifty-five Klebsiella isolates from dogs with clinical infections were identified by MALDI‒TOF MS. Antimicrobial susceptibility was assessed by disk diffusion. ESBL and carbapenemase genes were detected by PCR. Biofilm formation was evaluated using a microtiter plate assay. The only blaKPC-positive isolate underwent whole-genome sequencing and phylogenetic analysis with publicly available Brazilian genomes.</p><p><strong>Results: </strong>Klebsiella pneumoniae was the predominant species (74.5%), followed by K. variicola (20%). Multidrug resistance was observed in 36.4% of the K. pneumoniae isolates. High resistance rates of the isolates were observed for fluoroquinolones, cephalosporins, and sulfonamides, whereas chloramphenicol showed moderate susceptibility. The most frequent β-lactamase genes were blaTEM-1 (34.5%) and blaCTX-M-15 (30.9%). A single isolate (1.8%) harbored blaKPC. No hypervirulence-associated genes were detected. All the isolates formed biofilms, predominantly with weak to moderate adhesion. Whole-genome sequencing of the blaKPC-positive isolate revealed K. pneumoniae ST11 (CG340) carrying multiple resistance genes, including blaKPC-2, and clustering with human-associated genomes within a high-risk lineage.</p><p><strong>Conclusions: </strong>Multidrug-resistant K. pneumoniae, including a high-risk ST11 blaKPC-2-producing clone, was identified in diseased dogs, supporting the need to investigate companion animals as possible reservoirs of clinically relevant antimicrobial resistance determinants. The combination of multidrug resistance and biofilm formation highlights the importance of continued surveillance and prudent antimicrobial use within One Health.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148669512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simultaneous inhibition of Microcystis growth, toxin production, and release by a high-efficacy algicidal bacterium Aeromonas sp. N3.","authors":"Di Luo, Xiaotong Shen, Haixiao Dai, Jieming Li","doi":"10.1093/jambio/lxag128","DOIUrl":"10.1093/jambio/lxag128","url":null,"abstract":"<p><strong>Aims: </strong>This study aimed to isolate an algicidal bacterium able to strongly inhibit Microcystis growth and microcystins (MCs) production/release simultaneously, and to consolidate bacterial efficacy for potential practice.</p><p><strong>Methods and results: </strong>Algicidal bacterium Aeromonas sp. N3 effectively inhibited growth of 105 [non-quorum sensing (QS)-activated] and 108 cells/mL (QS-activated) Microcystis by 98.95% and 90.22% by day 26, respectively, and sharply decreased extracellular and intracellular MCs by > 90% for both densities, as detected by enzyme-linked immunosorbent assay. Quantitative polymerase chain reaction revealed algicidal effects involved stably strong downregulation of algal energy acquisition, nutrient assimilation, DNA repair, and heat-shock response. N3 exerted algicidal effect via excreted algicidal compounds, as its primary filtrates also stably suppressed Microcystis growth and MC-production/release with inferior efficacy than free-living N3, while its secondary filtrate with pre-exposure to Microcystis showed weaker efficacy. To consolidate N3's application potential, its immobilized agent was optimally constructed by dropping procedures with orthogonal tests using various polyvinyl alcohol-sodium alginate ratios. Alike to free-living N3, immobilized agent with equivalent cell loading posed equally strong inhibitory effects on Microcystis growth (> 90% inhibition) and extracellular/intracellular MCs content (> 95% inhibition), thus diminishing MC-pollution by blocking MC-synthesis/release, which is superior to by degrading aqueous MCs after release.</p><p><strong>Conclusions: </strong>Aeromonas sp. N3 had functional robustness and stability against Microcystis growth and MC-pollution, regardless of Microcystis density, exhibiting robust potential for mitigating both low- and high-density toxic Microcystis-dominated cyanobacterial blooms (corresponding to early- and mature-stage MCBs). Optimal construction of immobilized agent further consolidated its applicability in effective MCBs and MC-pollution mitigation.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148149929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Staged microbial consortium valorizes cattle manure wastewater and promotes maize growth.","authors":"Xiaoling Wang, Jiawei Cao, Longkang Ni, Hongfei Zhao, Liju Zhou, Youyou Wang","doi":"10.1093/jambio/lxag151","DOIUrl":"10.1093/jambio/lxag151","url":null,"abstract":"<p><strong>Aims: </strong>Ammonia-oxidizing bacteria play important roles in nitrogen transformation and agricultural-waste valorization; however, their large-scale application is often limited by high cultivation costs and insufficient functional stability. This study aimed to develop a low-cost and stable microbial consortium for converting cattle manure wastewater into a bioactive fermentation broth and to investigate its underlying mechanisms.</p><p><strong>Methods and results: </strong>The heterotrophic ammonia-oxidizing bacterium Ensifer sp. S2-8-1 was used as the core strain, and Bacillus subtilis was employed as the auxiliary strain. Three cultivation systems were compared: S2-8-1 monoculture, simultaneous co-culture, and a staged consortium (SYC). Among them, SYC exhibited the best overall performance, maintaining the highest viable bacterial density and reaching 3.93 × 107 CFU ml-1 on day 12. In a soil-maize pot experiment, SYC significantly enhanced rhizosphere nitrification, increased NO3--N availability, and promoted maize growth, resulting in a 41.4% increase in whole-plant dry matter compared with the water control. The consortium also improved photosynthetic performance and cytokinin accumulation. Comparative metatranscriptomic analysis revealed significant upregulation of heterotrophic ammonia oxidation-related genes (CYP450) and cytokinin-related genes (miaA and LOG) in S2-8-1 under SYC conditions, suggesting coordinated activation of nitrogen transformation and plant growth-promoting signaling pathways.</p><p><strong>Conclusions: </strong>The staged \"pre-modification followed by synergy\" consortium effectively converts cattle manure wastewater into a stable and bioactive fermentation broth, enhancing both microbial biomass production and plant growth-promoting activity. This strategy provides a scalable approach for livestock wastewater valorization and the development of compound microbial fertilizers.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148317247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating epigenetic memory and plant growth-promoting rhizobacteria-mediated signaling for climate-resilient agriculture.","authors":"Riyaz Sayyed, Mohammed Al-Zharani, Mohammed Mubarak, Nicoleta Anca Șuțan, Dilfuza Egamberdieva, Abhishek Sharma, Nazih Y Rebouh, Jayanthi Barasarathi","doi":"10.1093/jambio/lxag149","DOIUrl":"10.1093/jambio/lxag149","url":null,"abstract":"<p><strong>Aims: </strong>Climate change is shifting agriculture toward multifactorial abiotic stresses (drought, heat, and salinity). This study aims to characterize emergent, non-additive plant responses to combined stresses and to define the epigenetic and microbial frameworks that govern environmental memory and adaptive plasticity.</p><p><strong>Methods and results: </strong>We conducted a meta-synthesis of molecular and ecological studies, evaluating high-throughput data on DNA methylation, histone modifications, and ncRNA profiles. We further analyzed the plant holobiont to determine how rhizosphere and endosphere microbiota influence host stress imprinting. The analysis revealed that stress combinations trigger distinct transcriptomic and metabolic signatures, which are stabilized by an \"epigenetic toolkit\" such as RNA-directed DNA methylation and chromatin remodeling. Furthermore, plant-associated microbiota serve as an extrinsic regulatory layer, modulating host epigenetic states to prime plants for compound stress. While translational pathways such as epigenetic editing, Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) mediated epigenome editing, and microbiome engineering show promise, their field-scale stability remains context-dependent.</p><p><strong>Conclusion: </strong>Building climate resilience requires a paradigm shift from traditional single-trait breeding toward multi-scale regulatory approaches. Harnessing the synergy between the plant epigenome and the microbiome enables the development of 'primed' crop varieties-an integrated strategy vital for safeguarding global food security amid intensifying environmental volatility.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148308087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactive environments to combat antimicrobial resistance: artificial intelligence and model-driven microbial biocontrol for living materials.","authors":"Kathleen L Furtado, Jack A Gilbert, Maxwell Neal","doi":"10.1093/jambio/lxag161","DOIUrl":"10.1093/jambio/lxag161","url":null,"abstract":"<p><p>Antimicrobial resistance (AMR) continues to outpace development of new therapeutics. Many interventions focus on treating infection after it occurs, but resistant pathogens often emerge, persist, and spread within reservoirs, such as built environments. Microbial biocontrol offers a complementary, upstream strategy by reshaping ecological interactions to suppress the colonization, persistence, and transmission of AMR pathogens. Currently, biocontrol design relies upon the presumed functionality of probiotic genera across diverse environments despite limited experimental validation, alongside heuristic model predictions that prioritize efficiency over sensitivity. These approaches yield inconsistent outcomes, reflecting the context-dependent nature of microbial behavior. We review how advances in metabolic modeling and artificial intelligence (AI), in conjunction with experimental data, enable adaptable, context-aware biocontrol design with iterative design-test-learn cycles for optimization. We outline the ecological principles underlying microbial competition, highlighting Bacillus as a robust biocontrol chassis due to its biosynthetic capacity, stress tolerance, and genetic tractability. We then discuss how genome-scale, pan-genome-scale, and metabolism-and-expression models provide mechanistic insight into competitive fitness, metabolic trade-offs, and persistence. AI advances these approaches by extracting patterns from multi-omic datasets to build specific, yet versatile, foundation models that guide strain and/or consortium selection for specific built environments. Moreover, these tools facilitate safe biocontrol deployment by enabling risk assessment of persistence, ecological displacement, and horizontal gene transfer, particularly for engineered living materials and bioactive building surfaces. Ultimately, AI-guided modeling and systems-level design provide scalable frameworks for developing durable, preventive strategies against AMR, shifting the focus from reactive treatment toward proactive control of pathogen ecology.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148396804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selective human trypsin inhibitors from cyanobacteria.","authors":"Xiaodan Ouyang, Muhammad N Ahmed, Matti Wahlsten, Tania K Shishido, Jouni Jokela, Kaarina Sivonen, David P Fewer, Hannu Koistinen","doi":"10.1093/jambio/lxag167","DOIUrl":"10.1093/jambio/lxag167","url":null,"abstract":"<p><strong>Aims: </strong>Aberrant activity of human trypsin isoenzymes has been linked to diseases like cancer. The high structural and catalytic similarity among the three human trypsin isoenzymes makes selective inhibition of individual trypsin isoenzymes particularly challenging. As cyanobacteria are a prolific source of structurally diverse serine protease inhibitors with potential pharmaceutical applications, we studied them as producers of selective human trypsin inhibitors.</p><p><strong>Methods and results: </strong>Here, we evaluated aqueous extracts of 505 strains of cyanobacteria, isolated from the Baltic Sea and Finnish lakes, for inhibitory activity against human trypsin isoenzymes. Extracts from 98 strains (19% of those tested), belonging primarily to the genera Microcystis, Nodularia, Nostoc, Oscillatoria, and Planktothrix, exhibited inhibitory activity against one or more trypsin isoenzymes. Twenty-four extracts preferentially inhibited trypsin-1 and trypsin-2, eight inhibited both trypsin-2 and trypsin-3, and seven showed selectivity toward trypsin-2 alone. We selected eight extracts for further bioactivity tests against plasma-KLK, bovine trypsin, Factor Xa, and thrombin. While all of these extracts inhibited bovine trypsin, some also inhibited plasma-KLK, thrombin, or Factor Xa. Molecular networking of high-resolution mass spectrometry data indicated the presence of both known trypsin inhibitors and potentially novel metabolites, suggesting that cyanobacteria represent a rich source of natural products with selective activity against human trypsin isoenzymes.</p><p><strong>Conclusions: </strong>Together, these results suggest that cyanobacteria harbor a wealth of metabolites with selective inhibitory activity against human trypsin isoenzymes that could serve as a starting point for the development of protease inhibitors.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148396740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acetic acid stress induces resistance and growth adaptation of Bacillus weihenstephanensis KBAB4.","authors":"Alexandra Rahmani, Elvin Goyat, Fabien Saubade, Olivier Couvert, Louis Coroller, Noémie Desriac","doi":"10.1093/jambio/lxag148","DOIUrl":"10.1093/jambio/lxag148","url":null,"abstract":"<p><strong>Aims: </strong>Bacillus weihenstephanensis KBAB4 is a Gram-positive bacterial strain belonging to the group Bacillus cereus. In this study, the impact of the physiological state on acetic acid resistance and on growth limits was investigated.</p><p><strong>Methods and results: </strong>Strain KBAB4 was cultivated in BHI medium to produce reference cells (BHI, pH 7.2), HCl-adapted cells (BHI, pH 5.5), or acetic acid-adapted cells (BHI, pH 5.5+14 mM of acetic acid). The bacterial resistance of these cells was measured by inactivation, monitoring the reduction of the bacterial concentration in BHI, pH 5.5 + 150 mM of acetic acid. The growth limits of reference or acid-adapted cells were assessed on 96-well plates with BHI medium supplemented with HCl (pH 4.6 to 5.2) or acetic acid (from 14 mM to 150 mM). The acid resistance (δ, i.e. the first decimal decrease) during inactivation increased almost 3-fold for the HCl-adapted cells and 5-fold for the acetic acid-adapted cells, compared to the reference cells. The pH growth limits and the minimal inhibitory concentration of acetic acid were not affected by HCl adaptation, whereas they were modified for acetic acid-adapted cells.</p><p><strong>Conclusions: </strong>For strain KBAB4, within an isogenic population, the bacterial life history influences the inactivation time during lethal acetic acid exposure, as well as the growth limits. This raises the question of whether this factor should be considered in the assessment of microbiological risks in food safety models for other B. cereus strains, since variability in the physiological state could be a decisive factor influencing food safety.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148277516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Basil essential oil induces multifactorial stress in Klebsiella variicola: a potential natural strategy to control a foodborne pathogen.","authors":"Valentina Pavone, Ivanka Krasteva, Maria Schirone, Clemencia Chaves López, Francisco Emilio Argote-Vega, Lisa Di Marcantonio, Nicola D'Alterio, Flavio Sacchini, Antonello Paparella, Mirella Luciani","doi":"10.1093/jambio/lxag152","DOIUrl":"10.1093/jambio/lxag152","url":null,"abstract":"<p><strong>Aims: </strong>Klebsiella variicola, traditionally considered a plant-associated bacterium, is emerging as a foodborne and opportunistic pathogen with growing antimicrobial resistance. Its persistence in food and food-processing environments represents a potential risk to public health. This study aimed to investigate how basil (Ocimum basilicum) essential oil (BEO), a plant-derived antimicrobial, affects the physiology of K. variicola and to identify molecular targets that could reduce its persistence in food-related environments.</p><p><strong>Methods and results: </strong>We applied a label-free quantitative proteomic approach to examine the global responses of K. variicola to a subinhibitory concentration of BEO. Exposure to BEO induced multifactorial stress responses, including altered membrane homeostasis, oxidative management, and translation processes. Key energy-related pathways were downregulated, while antioxidant defenses were upregulated, indicating that BEO imposes combined physiological stresses rather than acting through a single mechanism.</p><p><strong>Conclusions: </strong>These findings provide the first proteome-level insight into the cellular response of K. variicola to essential oils and highlight molecular vulnerabilities that could be exploited to limit its survival in food-processing settings. Overall, the study supports the potential of BEO as a natural antimicrobial strategy to improve food safety.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148338714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-omics analysis identify novel microbiome-metabolome signatures associated with obesity.","authors":"Bo Tian, Yong Liu, Kuan-Jui Su, Lin-Dong Jiang, Xu Lin, Chuan Qiu, Zhe Luo, Qing Tian, Jie Shen, Hui Shen, Li-Shu Zhang, Hong-Mei Xiao, Hong-Wen Deng","doi":"10.1093/jambio/lxag172","DOIUrl":"10.1093/jambio/lxag172","url":null,"abstract":"<p><strong>Aims: </strong>Explore the potential microbiome and serum metabolome factors and their interactions associated with obesity.</p><p><strong>Methods and results: </strong>We performed a systematic multi-omics analysis using paired metagenomic and metabolomic profiles-including untargeted serum metabolomics, lipidomics, and short-chain fatty acids (SCFAs)-with body mass index (BMI) from a cohort of 495 US men. Single omics analysis identified 52 gut bacteria species and 31 serum metabolites for potential associations with BMI. Among the identified bacteria, Collinsella stercoris (C. stercoris) (Coef.=-0.147, P = 0.015) was negatively associated, whereas Bacteroides fragilis (B. fragilis) (Coef.=0.294, P = 1.22E-04) and Veillonella dispar (V. dispar) (Coef.=0.135, P = 0.001) were positively associated, these results were further validated by an independent Chinese cohort. Several of the identified metabolites, including gamma-glutamylglycine (Coef.=-0.713, P = 4.53E-06), asparagine (Coef.=-0.629, P = 3.53E-05), glycine (Coef.=-0.952, P = 5.28E-09), and serotonin (Coef.=0.566, P = 1.78E-04) were associated with these significant bacteria (P < 0.05).</p><p><strong>Conclusion: </strong>This multi-omics study identifies key gut bacteria and serum metabolites that interact to associate with host obesity, providing systemic insight into microbiome-host metabolic interactions.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148421340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}