Rosy Khatoon, Andrzej Skwarecki, Ryszard Andruszkiewicz, Amandeep Saini, Sarita Malik, Rajendra Prasad, Amresh Prakash, Sławomir Milewski, Atanu Banerjee
{"title":"The proton-dependent oligopeptide transporter Ptr_C serves as an efficient system for antifungal delivery into Candida auris.","authors":"Rosy Khatoon, Andrzej Skwarecki, Ryszard Andruszkiewicz, Amandeep Saini, Sarita Malik, Rajendra Prasad, Amresh Prakash, Sławomir Milewski, Atanu Banerjee","doi":"10.1093/jambio/lxaf170","DOIUrl":"10.1093/jambio/lxaf170","url":null,"abstract":"<p><strong>Aims: </strong>PTR transporters mediate the uptake of dipeptides/tripeptides as well as peptidomimetic drugs across diverse organisms, including bacteria, yeast, and humans. Our previous study identified three PTR transporters in Candida auris, with Ptr_C serving as a key player in the uptake of peptide substrates, including the antifungal, l-norvalyl-N3-(4-methoxyfumaroyl)-l-2,3-diaminopropanoic acid (Nva-FMDP). This study aims to evaluate the efficacy of different FMDP-based antifungal variants against C. auris, investigate the contribution of Ptr_C in their uptake, and establish a heterologous system to screen for peptide-based inhibitors that exploit Ptr_C for cellular entry.</p><p><strong>Methods and results: </strong>In this study, utilizing deletion mutants of C. auris PTR transporters, we demonstrate that Ptr_C is the primary transporter facilitating the uptake of FMDP-based antifungal peptides. Furthermore, we developed a Ptr_C overexpression system in Saccharomyces cerevisiae, enabling rapid identification of antifungal peptides capable of exploiting this transporter using simplistic growth-based assays. The system's competence was further validated by constructing a few key site-directed mutants that alter the functional specificity of Ptr_C.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144583965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Wanda Antonia Flegler, Freya Behrens, Anne Theresa Sturmhöfel, Miriam Sonntag, Lisa Schmitt, Kristin Rang, Hauke Ferdinand Deeken, Yurui Sun, Gerd-Christian Maack, Wolfgang Büscher, Mareike Baer, André Lipski
{"title":"Long-read 16S amplicon analyses and improved cultivation techniques as joined approach for the identification of viable bacterial populations in silage.","authors":"Wanda Antonia Flegler, Freya Behrens, Anne Theresa Sturmhöfel, Miriam Sonntag, Lisa Schmitt, Kristin Rang, Hauke Ferdinand Deeken, Yurui Sun, Gerd-Christian Maack, Wolfgang Büscher, Mareike Baer, André Lipski","doi":"10.1093/jambio/lxaf151","DOIUrl":"10.1093/jambio/lxaf151","url":null,"abstract":"<p><strong>Aims: </strong>Lactic acid bacteria (LAB) are crucial for the quality and preservation of silage. Exploration and isolation of the key microbiome are important for improving fermentation processes. While cultivation-dependent methods enable the isolation of LAB, they often overlook organisms with challenging cultivation requirements. Next-generation sequencing provides cultivation-independent microbiome insights. In contrast to commonly used methods, long-read sequencing platforms like the Pacific Biosciences (PacBio) single-molecule real-time (SMRT) platform offer species-level resolution. This study aims to investigate the benefits of a combined cultivation-dependent and -independent approach for silage microbiome analyses.</p><p><strong>Methods and results: </strong>The common isolation medium for LAB was supplemented with sterile pressed juice from plant material improving the cultivation conditions. PacBio amplicon sequencing provided an almost complete and cultivation-independent picture of the bacterial community. The use of mock communities and a live/dead discriminating treatment of the samples revealed that the analysis can be misleading if appropriate controls are not performed.</p><p><strong>Conclusion: </strong>Growth of plant-associated microorganisms can be supported by a plant juice containing isolation medium, allowing isolation of the dominant LAB from silage. Microbial dynamics can be represented using long-read sequencing. However, the use of controls such as a live/dead discrimination and mock communities is essential for the reliability of the data.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144293802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Claire Thom, Umer Ijaz, Graeme Moore, Paul Weir, Cindy J Smith
{"title":"Evaluation of existing lacZ primers and de novo design of an optimized qPCR assay to quantify coliform bacteria in drinking water.","authors":"Claire Thom, Umer Ijaz, Graeme Moore, Paul Weir, Cindy J Smith","doi":"10.1093/jambio/lxaf156","DOIUrl":"10.1093/jambio/lxaf156","url":null,"abstract":"<p><strong>Aims: </strong>This study aimed to evaluate existing and de novo lacZ primers using in silico and experimental validation to develop a quantitative polymerase chain reaction (qPCR) assay capable of reliably quantifying coliforms and differentiating them from non-coliform Enterobacteriaceae as currently defined.</p><p><strong>Methods and results: </strong>A comprehensive lacZ sequence database was compiled to define coliform and non-coliform targets. Both published and de novo primers were assessed for specificity and coverage. The de novo primer set LZ1 (F: CCGWGYRTKATCATCTGGTC, R: TSATCSACGCGSGCGTACAT; 173 bp amplicon) showed 87.5% coverage of the test panel and was optimal for qPCR. Compared with culture-based methods and flow cytometry, LZ1 quantified Escherichia coli in drinking water at 1 × 10³ cfu 100 ml-1. The limit of quantification indicated an 80% probability of detecting 100 copies with > 3 replicates. Existing primer LZ3 best distinguished coliforms from non-coliforms and is a promising target for identification.</p><p><strong>Conclusions: </strong>We present a validated qPCR assay targeting the lacZ gene, supported by in silico and experimental validation, and a phylogenetic analysis of lacZ and 16S rRNA sequences, highlighting the challenges associated with coliform detection.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Belinda E Stummer, Minoo J Moghaddam, Mahshid Roohani-Dezfouli, Bhanu Nidumolu, Xinjian Zhang, Paul R Harvey
{"title":"Wheat rhizosphere persistence of Trichoderma gamsii A5MH during suppression of a Fusarium-Pythium root disease complex differentially impacts the soil fungal and oomycete microbiome.","authors":"Belinda E Stummer, Minoo J Moghaddam, Mahshid Roohani-Dezfouli, Bhanu Nidumolu, Xinjian Zhang, Paul R Harvey","doi":"10.1093/jambio/lxaf158","DOIUrl":"10.1093/jambio/lxaf158","url":null,"abstract":"<p><strong>Aims: </strong>Determine the impacts of inoculant strain Trichoderma gamsii A5MH and crop phenology on the structure of fungal and oomycete communities in wheat rhizosphere soil.</p><p><strong>Methods and results: </strong>Over two consecutive wheat crops, A5MH inoculation suppressed an oomycete (Globisporangium)-fungal (Fusarium) root disease complex. Amplicon sequencing determined the impacts of A5MH treatment and crop phenology on the structure of rhizosphere soil fungal and oomycete communities. Culture-dependent (C-D) techniques quantified inoculant impacts on non-target root endophytic fungi, previously co-isolated with strain A5MH and the fungal and oomycete pathogens. Inoculant treatment differentiated the structure of the fungal microbiome in both years, primarily due to increased Trichoderma abundance and decreases in cereal pathogenic, root endophytic, and saprophytic taxa. Strain A5MH did not impact the structure of the oomycete microbiome. Crop phenology altered fungal and oomycete community structure, these impacts greater at tillering and grain harvest, respectively. A5MH-induced decreases in rhizosphere abundance (C-D) of root endophytic fungi were associated with increased crop biomass at tillering.</p><p><strong>Conclusions: </strong>While the structure of rhizosphere soil fungal and oomycete communities altered as the wheat crop matured, only fungal communities were impacted by A5MH treatment due to increased Trichoderma and decreased abundance of recognized and emerging plant pathogenic fungi.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Synergistic effects of antibiotics and efflux pump inhibitors on multidrug-resistant Escherichia coli and Klebsiella pneumoniae.","authors":"Barani Thillai Devi, Vankadari Aditya, Sudarshan Kini, Ballamoole Krishna Kumar, Vijaya Kumar Deekshit","doi":"10.1093/jambio/lxaf169","DOIUrl":"10.1093/jambio/lxaf169","url":null,"abstract":"<p><strong>Aim: </strong>The increasing prevalence of multidrug-resistant Escherichia coli and Klebsiella pneumoniae presents a critical threat to public health. As a first line of defence, efflux pumps promote antibiotic resistance among pathogens. This study aims to assess the potential of efflux pump inhibitors (EPIs) as adjuvants for enhancing antibiotic efficacy against resistant pathogens.</p><p><strong>Methods and results: </strong>This research investigated antibiotic resistance patterns, antibiotic resistance genes, and the effects and expression profiles of efflux pump genes. The study examined bacterial responses to sub-inhibitory levels of antibiotics, both individually and in conjunction with the EPI. The real-time PCR/Quantitative PCR (qPCR) demonstrated that antibiotic exposure led to an upregulation of efflux genes in E. coli and K. pneumoniae, with significant strain-specific differences. The co-administration of EPI resulted in downregulation of efflux genes, indicating successful inhibition of the pumps. Gene expression analyses suggested that PAβN influences the regulation of efflux genes and may trigger oxidative stress responses mediated by global regulators. This implies that EPIs could directly and indirectly affect bacterial physiology.</p><p><strong>Conclusions: </strong>The results highlight the therapeutic promise of EPIs in combating antibiotic resistance. This study underscores the complexity of efflux-mediated resistance and the need for integrated strategies to mitigate MDR in Gram-negative pathogens.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Phuong Nguyen Tran, Agnes A Michalczyk, Rainier A Catubig, Jess Glasson, Jhonatan Soto Puelles, Mahdi Ghorbani, Anthony E Somers, Maria Forsyth, Margaret Leigh Ackland
{"title":"Corrosion of AISI 1030 mild steel is influenced by bacteria type, oxygen availability, and biofilm formation under controlled laboratory conditions.","authors":"Phuong Nguyen Tran, Agnes A Michalczyk, Rainier A Catubig, Jess Glasson, Jhonatan Soto Puelles, Mahdi Ghorbani, Anthony E Somers, Maria Forsyth, Margaret Leigh Ackland","doi":"10.1093/jambio/lxaf154","DOIUrl":"10.1093/jambio/lxaf154","url":null,"abstract":"<p><strong>Aim: </strong>Bacteria are reported to have both stimulatory and inhibitory effects on the corrosion of metal. To investigate this, we measured corrosion of AISI 1030 mild steel by four species of bacteria: Pseudomonas aeruginosa, Lelliottia WAP21, Bacillus subtilis, and Enterobacter cloacae in cultures with normal and restricted access to O2.</p><p><strong>Methods and results: </strong>Scanning electron microscopy, three-dimensional profilometry and inductively coupled plasma-mass spectrometry were used to measure corrosion. Under aerobic conditions, all four bacterial strains protected the metal surface from pit formation compared with abiotic cultures, most likely through the formation of a biofilm that restricting oxygen access. In contrast, in low-oxygen environments, bacteria caused greater surface corrosion and biofilm formation. Specifically, Lelliottia WAP21 caused corrosion pits more than 10 times deeper than those in abiotic cultures and 18-fold more Fe release relative to abiotic controls. Biofilm structures varied with oxygen availability, with each bacterial strain producing distinct biofilms with different elemental composition compared with the abiotic corrosion products. The O2 utilization in the presence of metal may be related to bacterial metabolic activities including biofilm formation. The presence of Fe was metabolically favourable for bacteria and stimulated growth particularly in low O2 conditions.</p><p><strong>Conclusion: </strong>Our findings show species-specific effects of bacteria on corrosion, where bacterial activity can either enhance or inhibit corrosion depending on oxygen availability.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Veridianna C Pattini, Leticia R de Assis, Margarete T G de Almeida, Luis O Regasini, Henny C van der Mei
{"title":"2-hydroxy-dibenzylideneacetone as a multifunctional coating inhibiting biofilm formation of Candida albicans.","authors":"Veridianna C Pattini, Leticia R de Assis, Margarete T G de Almeida, Luis O Regasini, Henny C van der Mei","doi":"10.1093/jambio/lxaf155","DOIUrl":"10.1093/jambio/lxaf155","url":null,"abstract":"<p><strong>Aim: </strong>Candida albicans emerges as an opportunistic fungus among nosocomial infections, which can include sepsis, bloodstream infections, and infections associated with medical devices. Therefore, we investigated the effectiveness of 2-hydroxy-dibenzylideneacetone, a monocarbonyl curcuminoid, as an antifouling coating against C. albicans.</p><p><strong>Methods and results: </strong>Polystyrene (hydrophobic) and glass (hydrophilic) were used to study the influence of a curcuminoid coating on biofilm formation. Fourier-transform-infrared-spectroscopy spectra indicated a successful curcuminoid coating on both surfaces. Atomic-force-microscopy data showed that the curcuminoid coating on polystyrene decreased the adhesion strength of C. albicans but had minimal effect on glass. Hyphal growth, a key virulence factor, was significantly reduced on both coated surfaces. Biofilm formation was significantly reduced on coated polystyrene, but not on glass. Gene expression revealed downregulation of adhesion and biofilm-related genes (HWP1, ALS3, ALS5). Exposure of a 48-h biofilm to curcuminoid decreased the metabolic activity of the biofilm. The curcuminoid coating was biocompatible and non-cytotoxic against human oral keratinocytes and human gingival fibroblasts.</p><p><strong>Conclusions: </strong>This study underscores the potential of a curcuminoid coating to prevent C. albicans adhesion, reduce hyphal and biofilm formation, and reduce metabolic activity of biofilms, highlighting its potential use as an antifouling coating against Candida-associated infections.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zuzanna Bacińska, Daniel Jan Strub, Lucyna Balcerzak
{"title":"Antibacterial potential of essential oils against oral pathogenic bacteria: a literature and clinical review.","authors":"Zuzanna Bacińska, Daniel Jan Strub, Lucyna Balcerzak","doi":"10.1093/jambio/lxaf161","DOIUrl":"10.1093/jambio/lxaf161","url":null,"abstract":"<p><p>The human oral microbiome is a complex ecosystem, comprising diverse microbial species in symbiotic relationships. Environmental factors such as diet, immune response, and inflammation can disrupt the balance of the oral microbiome, leading to increased activity and proliferation of pathogenic species associated with oral diseases. In response to global dental problems, there is a burgeoning interest in exploiting the antibacterial properties of essential oils (EOs) for clinical applications, being a promising alternative to traditional antiseptics. This review synthesizes the literature on the minimum inhibitory concentration of plant-derived EOs and their effectiveness against key oral pathogenic bacteria, which belong to the so-called \"red,\" \"orange,\" \"purple,\" \"yellow,\" and \"green complexes,\" and includes newly discovered oral bacteria. Furthermore, it examines clinical investigations into oral hygiene products infused with EOs, evaluating their antiplaque, antigingivitis properties, and effectiveness in reducing tartar formation and gingival bleeding. Overall, this review highlights the high antibacterial efficacy of EOs against oral bacteria and their potential therapeutic abilities. It is expected that they will be used as a potential alternative for chemical preservatives in oral care products in the future. Based on the searched clinical studies, EO-based oral care products seem to be effective in the treatment of dental problems, e.g. dental plaque, gingivitis, and caries.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144505780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Abundance and distribution of complete ammonia oxidizers under a salinity gradient in the Yangtze Estuary and its adjacent sea.","authors":"Qiu-Yue Jiang, Yi-Xuan Zhang, Dan-Qi Wang, Sheng-Nan Zhang, Jian-Gong Wang, Zhe-Xue Quan","doi":"10.1093/jambio/lxaf167","DOIUrl":"10.1093/jambio/lxaf167","url":null,"abstract":"<p><strong>Aims: </strong>This study investigated the distribution, community composition, and environmental drivers of complete ammonia-oxidizing bacteria (comammox) in estuarine sediments, focusing on the Yangtze River Estuary. It examined how environmental gradients-particularly salinity, pH, and ammonium concentration-influence comammox abundance and diversity, thereby clarifying their ecological niche in transitional aquatic ecosystems.</p><p><strong>Methods and results: </strong>Seventeen sediment samples were collected across the estuary. Quantitative Polymerase Chain Reaction and high-throughput sequencing revealed that comammox clade A.2 dominated the community. Salinity showed a strong negative correlation with comammox abundance, while ammonia-oxidizing bacteria and ammonia-oxidizing archaea abundance increased with rising salinity. Comammox was the predominant ammonia oxidizer in low-salinity sediments, with its abundance also shaped by pH and ammonium levels.</p><p><strong>Conclusions: </strong>Comammox is widespread and important contributors to nitrification in Yangtze Estuary estuarine sediments, especially under low-salinity conditions. Their community structure and abundance are primarily shaped by salinity, indicating a distinct ecological niche compared to canonical ammonia oxidizers.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144583963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Huanhuan Zhang, Linmei Li, Siqi Yang, Bowei Xue, Shangbo Yan, Chun Hu, Qing Li, Weishou Shen, Nan Gao
{"title":"Effect of three rhizobacteria on lettuce growth and soil N2O emission and their impact on the rhizosphere bacterial community in acidic field experiments.","authors":"Huanhuan Zhang, Linmei Li, Siqi Yang, Bowei Xue, Shangbo Yan, Chun Hu, Qing Li, Weishou Shen, Nan Gao","doi":"10.1093/jambio/lxaf162","DOIUrl":"10.1093/jambio/lxaf162","url":null,"abstract":"<p><strong>Aims: </strong>Acidic soils have a high potential for nitrous oxide (N2O) emission. Plant growth-promoting rhizobacteria (PGPR) may mitigate N2O emissions in acidic soils; however, the reduction capacity and their microbiological mechanisms are poorly understood. We investigated the effects of three PGPR strains (Bacillus subtilis subsp. subtilis NRCB002, Stutzerimonas stutzeri NRCB010, and B. velezensis NRCB026) on crop growth and N2O emissions in acidic soils, and explored their microbial mechanisms.</p><p><strong>Methods and results: </strong>A field experiment was conducted to assess the effects of inoculation with NRCB002, NRCB010, and NRCB026 on lettuce growth and N2O emissions from acidic soil and to study the associated microbiological mechanisms. The results indicated that inoculation with NRCB002, NRCB010, and NRCB026 significantly promoted lettuce growth and decreased N2O emissions from soil by 16.3%, 36.2%, and 45.8%, respectively. Furthermore, inoculation with these three strains altered the microbial community composition and nitrogen cycle functional gene abundance in acidic soils. Structural equation models indicated that inoculation with these three strains affected N2O emissions from the soil by modulating soil pH, relative abundance of Proteobacteria, and nosZ gene copy numbers. N2O emissions from soil inoculated with NRCB002 were negatively correlated with nosZI gene copy number, while NRCB010 and NRCB026 were negatively correlated with nosZII gene copy number.</p><p><strong>Conclusions: </strong>PGPR strains enhanced lettuce growth and decreased acidic soil N2O emissions under field conditions. Among the three strains, NRCB026 significantly decreased N2O emission from acidic soil.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144540331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}