Journal of Applied Microbiology最新文献_第9页

Molecular and biochemical characterization of a plant-like iota-class glutathione S-transferase from the halotolerant cyanobacterium Halothece sp. PCC7418. 耐卤蓝藻 Halothece sp.PCC7418。

IF 3.2 3区生物学

Journal of Applied Microbiology Pub Date : 2024-09-02 DOI: 10.1093/jambio/lxae230

Sasiprapa Samsri, Chananwat Kortheerakul, Hakuto Kageyama, Rungaroon Waditee-Sirisattha

{"title":"Molecular and biochemical characterization of a plant-like iota-class glutathione S-transferase from the halotolerant cyanobacterium Halothece sp. PCC7418.","authors":"Sasiprapa Samsri, Chananwat Kortheerakul, Hakuto Kageyama, Rungaroon Waditee-Sirisattha","doi":"10.1093/jambio/lxae230","DOIUrl":"10.1093/jambio/lxae230","url":null,"abstract":"Aims: This study identifies a unique glutathione S-transferase (GST) in extremophiles using genome, phylogeny, bioinformatics, functional characterization, and RNA sequencing analysis.Methods and results: Five putative GSTs (H0647, H0729, H1478, H3557, and H3594) were identified in Halothece sp. PCC7418. Phylogenetic analysis suggested that H0647, H1478, H0729, H3557, and H3594 are distinct GST classes. Of these, H0729 was classified as an iota-class GST, encoding a high molecular mass GST protein with remarkable features. The protein secondary structure of H0729 revealed the presence of a glutaredoxin (Grx) Cys-Pro-Tyr-Cys (C-P-Y-C) motif that overlaps with the N-terminal domain and harbors a topology similar to the thioredoxin (Trx) fold. Interestingly, recombinant H0729 exhibited a high catalytic efficiency for both glutathione (GSH) and 1-chloro-2, 4-dinitrobenzene (CDNB), with catalytic efficiencies that were 155- and 32-fold higher, respectively, compared to recombinant H3557. Lastly, the Halothece gene expression profiles suggested that antioxidant and phase II detoxification encoding genes are crucial in response to salt stress.Conclusion: Iota-class GST was identified in cyanobacteria. This GST exhibited a high catalytic efficiency toward xenobiotic substrates. Our findings shed light on a diversified evolution of GST in cyanobacteria and provide functional dynamics of the genes encoding the enzymatic antioxidant and detoxification systems under abiotic stresses.","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142125801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Amoebicidal activity of essential oils and essential oil-based microemulsions of Aloysia citrodora Ortega ex Pers., Cymbopogon winterianus Jowitt ex Bor, and Ocimum gratissimum L. against Acanthamoeba polyphaga trophozoites. Aloysia citrodora Ortega ex Pers.、Cymbopogon winterianus Jowitt ex Bor 和 Ocimum gratissimum L.的精油和基于精油的微乳剂对多棘阿米巴滋养体的杀阿米巴活性。

IF 3.2 3区生物学

Journal of Applied Microbiology Pub Date : 2024-09-02 DOI: 10.1093/jambio/lxae232

Ingrid Borges Siqueira, Yrna Lorena Matos de Oliveira, Braulio Otomar Caron, Denise Schmidt, Audrey Rouse Soares Tavares Silva, Sona Jain, Jonatas Dos Santos de Souza, Victor Hugo Vitorino Sarmento, Suely Moraes Alves, Marilise Brittes Rott, Cristiane Bani Correa, Ricardo Scher, Ana Andrea Teixeira Barbosa, Silvio Santana Dolabella

{"title":"Amoebicidal activity of essential oils and essential oil-based microemulsions of Aloysia citrodora Ortega ex Pers., Cymbopogon winterianus Jowitt ex Bor, and Ocimum gratissimum L. against Acanthamoeba polyphaga trophozoites.","authors":"Ingrid Borges Siqueira, Yrna Lorena Matos de Oliveira, Braulio Otomar Caron, Denise Schmidt, Audrey Rouse Soares Tavares Silva, Sona Jain, Jonatas Dos Santos de Souza, Victor Hugo Vitorino Sarmento, Suely Moraes Alves, Marilise Brittes Rott, Cristiane Bani Correa, Ricardo Scher, Ana Andrea Teixeira Barbosa, Silvio Santana Dolabella","doi":"10.1093/jambio/lxae232","DOIUrl":"10.1093/jambio/lxae232","url":null,"abstract":"Aims: Evaluate the in vitro efficacy of the essential oils derived from Aloysia citrodora (Verbenaceae), Cymbopogon winterianus (Poaceae), and Ocimum gratissimum (Lamiaceae) against Acanthamoeba polyphaga trophozoites. Additionally, microemulsions formulated with these essential oils, along with their major components, were analyzed.Methods and results: The prepared microemulsions were characterized using polarized light microscopy and rheological techniques. The amoebicidal activity was determined by measuring the inhibitory concentration (IC50). Flow cytometry was employed to detect membrane damage and alterations in trophozoites size. The results revealed transparent and thermodynamically stable microemulsions. The essential oil from O. gratissimum exhibited a lower IC50, with values of 280.66 and 47.28 µg ml-1 after 24 and 48 h, respectively. When microemulsions containing essential oils were tested, the IC50 values exhibited a reduction of over 80% after 24 h. Particularly, eugenol, a constituent of the O. gratissimum essential oil, displayed higher amoebicidal activity. The essential oils also caused damage to the cell membrane, resulting in the subsequent death of the trophozoites.Conclusions: The EOs of A. citrodora, C. winterianus, and O. gratissimum and their microemulsions showed antiparasitic effect against A. polyphaga trophozoites, representing promising alternatives for the treatment of diseases caused by this protozoan.","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142140074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Specific gut microbiome's role in skin pigmentation: insights from SCARB1 mutants in Oujiang colour common carp. 特定肠道微生物群在皮肤色素沉着中的作用：从瓯江色鲤 SCARB1 突变体中获得的启示

IF 3.2 3区生物学

Journal of Applied Microbiology Pub Date : 2024-09-02 DOI: 10.1093/jambio/lxae226

Nusrat Hasan Kanika, Xin Hou, Hao Liu, Yue Dong, Jun Wang, Chenghui Wang

{"title":"Specific gut microbiome's role in skin pigmentation: insights from SCARB1 mutants in Oujiang colour common carp.","authors":"Nusrat Hasan Kanika, Xin Hou, Hao Liu, Yue Dong, Jun Wang, Chenghui Wang","doi":"10.1093/jambio/lxae226","DOIUrl":"10.1093/jambio/lxae226","url":null,"abstract":"Aims: Beyond the pivotal roles of the gut microbiome in initiating physiological processes and modulating genetic factors, a query persists: Can a single gene mutation alter the abundance of the gut microbiome community? Not only this, but the intricate impact of gut microbiome composition on skin pigmentation has been largely unexplored.Methods and results: Based on these premises, our study examines the abundance of lipase-producing gut microbes about differential gene expression associated with bile acid synthesis and lipid metabolism-related blood metabolites in red (whole wild) and white (whole white wild and SCARB1-/- mutant) Oujiang colour common carp. Following the disruption of the SCARB1 gene in the resulting mutant fish with white body colour (SCARB1-/-), there is a notable decrease in the abundance of gut microbiomes (Bacillus, Staphylococcus, Pseudomonas, and Serratia) associated with lipase production. This reduction parallels the downregulation seen in wild-type white body colour fish (WW), as contrasting to the wild-type red body colour fish (WR). Meanwhile, in SCARB1-/- fish, there was a downregulation noted not only at the genetic and metabolic levels but also a decrease in lipase-producing bacteria. This consistency with WW contrasts significantly with WR. Similarly, genes involved in the bile acid synthesis pathway, along with blood metabolites related to lipid metabolism, exhibited downregulation in SCARB1-/- fish.Conclusions: The SCARB1 knockout gene blockage led to significant alterations in the gut microbiome, potentially influencing the observed reduction in carotenoid-associated skin pigmentation. Our study emphasizes that skin pigmentation is not only impacted by genetic factors but also by the gut microbiome. Meanwhile, the gut microbiome's adaptability can be rapidly shaped and may be driven by specific single-gene variations.","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142145704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Actinomycin D reduces virulence factors and biofilms against Aeromonas hydrophila. 放线菌素 D 可减少嗜水气单胞菌的毒力因子和生物膜。

IF 3.2 3区生物学

Journal of Applied Microbiology Pub Date : 2024-09-02 DOI: 10.1093/jambio/lxae240

Yuexiang Zeng, Junsheng Liu, Wei Wang, Bo Wang, Aiqun Jia

{"title":"Actinomycin D reduces virulence factors and biofilms against Aeromonas hydrophila.","authors":"Yuexiang Zeng, Junsheng Liu, Wei Wang, Bo Wang, Aiqun Jia","doi":"10.1093/jambio/lxae240","DOIUrl":"10.1093/jambio/lxae240","url":null,"abstract":"Aims: Aeromonas hydrophila, a Gram-negative bacterium, is ubiquitously found in many aquatic habitats, causing septicemia in humans and fishes. Attributed to abuse or misuse of conventional antimicrobial drug usage, antimicrobial resistance is at an alarming rise. There is an available alternative strategy to bacterial resistance to antimicrobials, which is inhibition of virulence and pathogenicity employing quorum sensing inhibitors (QSIs). Hence, actinomycin D's effectiveness against A. hydrophila SHAe 115 as a QSI was investigated in decreasing virulence factors and preventing biofilm formation.Methods and results: Actinomycin D, belongs to the QSI combating Pseudomonas aeruginosa PAO1 originally isolated from an entophytic actinomycete (Streptomyces cyaneochromogenes RC1) in Areca catechu L. In the present work, further investigations were carried out to assess the effect of actinomycin D at subminimal inhibitory concentrations (sub-MICs), QS-regulated virulence factors, and biofilm inhibition strategies. Intrinsic properties encompassing inhibition of the production of protease and hemolysin and subsequent activities on biofilm formation and eradication of mature biofilm were established along with weakened swimming and swarming motilities in A. hydrophila SHAe 115. In the Tenebrio molitor survival assay, actinomycin D effectively reduced the virulence and pathogenicity of A. hydrophila, resulting in elimination of mortality. However, the hydrolysate of actinomycin D, 2-hydroxy-4,6-dimethyl-3-oxo-3H-phenoxazine-1,9-dicarboxylic acid (HDPD), had lost the QSI activity in A. hydrophila.Conclusions: Actinomycin D was proved as a viable QSI in lessening A. hydrophila's the virulence and pathogenicity, as evident from our research findings.","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142287924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacillus subtilis B55 degraded the ferulic acid and p-coumaric acid and changed the soil bacterial community in soils. 枯草芽孢杆菌 B55 降解了阿魏酸和对香豆酸，并改变了土壤中的细菌群落。

IF 3.2 3区生物学

Journal of Applied Microbiology Pub Date : 2024-09-02 DOI: 10.1093/jambio/lxae243

Dongli Liu, Xin Ge, Dandan Pan, Xianqing Zheng, Xingang Zhou

{"title":"Bacillus subtilis B55 degraded the ferulic acid and p-coumaric acid and changed the soil bacterial community in soils.","authors":"Dongli Liu, Xin Ge, Dandan Pan, Xianqing Zheng, Xingang Zhou","doi":"10.1093/jambio/lxae243","DOIUrl":"10.1093/jambio/lxae243","url":null,"abstract":"Aims: This study aimed to assess the effects of phenolic acid-degrading bacteria strains on phenolic acid content, plant growth, and soil bacterial community in phenolic acid-treated soils.Methods and results: The strain of interest coded as B55 was isolated from cucumber root litter, and its degradation rates of ferulic acid and p-coumaric acid were 81.92% and 72.41% in Luria-Bertani solution, respectively, and B55 was identified as Bacillus subtilis. B55 had plant growth-promoting attributes, including solubilization of inorganic phosphate and production of siderophore and indole acetic acid. Both ferulic acid and p-coumaric acid significantly restrained an increase in cucumber seedling dry biomass, while the B55 inoculation not only completely counteracted the damage of phenolic acids to cucumber seedlings and decreased the content of ferulic acid and p-coumaric acid in soil, but also promoted cucumber seedlings growth. Amplicon sequencing found that B55 inoculation changed the cucumber rhizosphere bacterial community structure and promoted the enrichment of certain bacteria, such as Pseudomonas, Arthrobacter, Bacillus, Flavobacterium, Streptomyces, and Comamonas.Conclusions: B55 not only promoted cucumber seedling growth, and decreased the content of ferulic acid and p-coumaric acid in soil, but it also increased the relative abundance of beneficial microorganisms in the cucumber rhizosphere.","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142287925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nematode-trapping fungus Arthrobotrys oligospora recruited rhizosphere microorganisms to cooperate in controlling root-knot nematodes in tomato. 诱捕线虫的真菌 Arthrobotrys oligospora 招募根瘤微生物合作控制番茄根结线虫。

IF 3.2 3区生物学

Journal of Applied Microbiology Pub Date : 2024-09-02 DOI: 10.1093/jambio/lxae218

Weixin Shen, Xinyue Yang, Yanzhuo Liu, Yongzhong Wang, Hengqian Lu

{"title":"Nematode-trapping fungus Arthrobotrys oligospora recruited rhizosphere microorganisms to cooperate in controlling root-knot nematodes in tomato.","authors":"Weixin Shen, Xinyue Yang, Yanzhuo Liu, Yongzhong Wang, Hengqian Lu","doi":"10.1093/jambio/lxae218","DOIUrl":"10.1093/jambio/lxae218","url":null,"abstract":"Aims: The objective of this study was to elucidate the role and mechanism of changes in the rhizosphere microbiome following Arthrobotrys oligospora treatment in the biological control of root-knot nematodes and identify the key fungal and bacterial species that collaborate with A. oligospora to biocontrol root-knot nematodes.Methods and results: We conducted a pot experiment to investigate the impact of A. oligospora treatment on the biocontrol efficiency of A. oligospora against Meloidogyne incognita infecting tomatoes. We analyzed the rhizosphere bacteria and fungi communities of tomato by high-throughput sequencing of the 16S rRNA gene fragment and the internal transcribed spacer (ITS). The results indicated that the application of A. oligospora resulted in a 53.6% reduction in the disease index of M. incognita infecting tomato plants. The bacterial diversity of rhizosphere soil declined in the A. oligospora-treated group, while fungal diversity increased. The A. oligospora treatment enriched the tomato rhizosphere with Acidobacteriota, Firmicutes, Bradyrhizobium, Sphingomonadales, Glomeromycota, and Purpureocillium. These organisms are involved in the utilization of rhizosphere organic matter, nitrogen, and glycerolipids, or play the role of ectomycorrhiza or directly kill nematodes. The networks of bacterial and fungal co-occurrence exhibited a greater degree of stability and complexity in the A. oligospora treatment group.Conclusions: This study demonstrated the key fungal and bacterial species that collaborate with the A. oligospora in controlling the root-knot nematode and elaborated the potential mechanisms involved. The findings offer valuable insights and inspiration for the advancement of bionematicide based on nematode-trapping fungi.","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142017518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacterial allelopathy: an approach for biological control of weeds. 细菌等位基因：生物防治杂草的一种方法。

IF 3.2 3区生物学

Journal of Applied Microbiology Pub Date : 2024-09-02 DOI: 10.1093/jambio/lxae219

Abubakar Dar, Zahir Ahmad Zahir, Maqshoof Ahmad, Azhar Hussain, Muhammad Tauseef Jaffar, Robert Jhon Kremer

{"title":"Bacterial allelopathy: an approach for biological control of weeds.","authors":"Abubakar Dar, Zahir Ahmad Zahir, Maqshoof Ahmad, Azhar Hussain, Muhammad Tauseef Jaffar, Robert Jhon Kremer","doi":"10.1093/jambio/lxae219","DOIUrl":"10.1093/jambio/lxae219","url":null,"abstract":"Weed infestation is one of the most damaging biotic factors to limit crop production by competing with the crop for space, water, and nutrients. Different conventional approaches are being used to cope with weed infestation, including labor intensive manual removal and the use of soil-degrading, crop-damaging, and environment-deteriorating chemical herbicides. The use of chemicals for weed control has increased 2-fold after the green revolution and their non-judicious use is posing serious threats to mankind, animals, and biodiversity. The detrimental effects of these approaches have shifted the researchers' attention from the last two decades towards alternate, sustainable, and eco-friendly approaches to cope with weed infestation. The recent approaches of weed control, including plant and microbial allelopathy have gained popularity during last decade. Farmers still use conventional methods, but the majority of farmers are very passionate about organic agriculture and describe it as a slogan in the developed world. The effectiveness of these approaches lies in host specificity by selective bacteria and differential response towards weeds and crops. Moreover, the crop growth promoting effect of microorganisms (allelopathic bacteria) possessing various growth promoting traits, that is, mineral solubilization, phytohormone production, and beneficial enzymatic activity, provide additional benefits. The significance of this review lies in the provision of a comprehensive comparison of the conventional approaches along with their potential limitations with advanced/biological weed control approaches in sustainable production. In addition, the knowledge imparted about weed control will contribute to a better understanding of biological control methods.","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142035927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sheep and goats as reservoirs of colistin-resistant E. coli: first detection of ETEC ST10 and E. coli ST6396 mcr-1 positive strains in North Africa. 绵羊和山羊是耐大肠菌素大肠杆菌的储存库：首次在北非发现 ETEC ST10 和大肠杆菌 ST6396 mcr-1 阳性菌株。

IF 3.2 3区生物学

Journal of Applied Microbiology Pub Date : 2024-09-02 DOI: 10.1093/jambio/lxae227

Fella Boukli-Hacene, Lydia Neïla Djouadi, Anis Raddaoui, Yousra Hachem, Hanane Boumerdassi, Wafa Achour, Farida Nateche

{"title":"Sheep and goats as reservoirs of colistin-resistant E. coli: first detection of ETEC ST10 and E. coli ST6396 mcr-1 positive strains in North Africa.","authors":"Fella Boukli-Hacene, Lydia Neïla Djouadi, Anis Raddaoui, Yousra Hachem, Hanane Boumerdassi, Wafa Achour, Farida Nateche","doi":"10.1093/jambio/lxae227","DOIUrl":"10.1093/jambio/lxae227","url":null,"abstract":"Aim: This study aimed to screen and characterize colistin-resistant strains isolated from different livestock species in Algeria, including sheep, goats, and dromedaries.Methods and results: A total of 197 rectal and nasal swabs were screened for colistin-resistant Gram-negative bacilli. Twenty one isolates were selected, identified, and their antibiotic resistance was phenotypically and genotypically characterized. The majority (15/21) were affiliated to Escherichia coli, from which 4 strains isolated from sheep (n = 2) and goats (n = 2) and belonging to phylogroup A and ST10 and ST6396 lineages, respectively, carried the mcr-1 gene. The remaining isolates were identified as belonging to the following genera: Raoultella, Enterobacter, Klebsiella, and Pseudomonas.Conclusion: This study highlights the presence of virulent and multiresistant Gram-negative bacilli in farm animals, increasing the risk of transmitting potentially fatal infections to humans.","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142119867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From crisis to cure: harnessing the potential of mycobacteriophages in the battle against tuberculosis. 从危机到治愈：利用噬分枝杆菌病毒的潜力抗击结核病。

IF 3.2 3区生物学

Journal of Applied Microbiology Pub Date : 2024-08-05 DOI: 10.1093/jambio/lxae208

Noura M Bin Yahia, Minghai Shan, Yue Zhu, Yuma Yang, Sihan Zhang, Yanhui Yang

{"title":"From crisis to cure: harnessing the potential of mycobacteriophages in the battle against tuberculosis.","authors":"Noura M Bin Yahia, Minghai Shan, Yue Zhu, Yuma Yang, Sihan Zhang, Yanhui Yang","doi":"10.1093/jambio/lxae208","DOIUrl":"10.1093/jambio/lxae208","url":null,"abstract":"Tuberculosis (TB) is a serious and fatal disease caused by Mycobacterium tuberculosis (Mtb). The World Health Organization reported an estimated 1.30 million TB-related deaths in 2022. The escalating prevalence of Mtb strains classified as being multi-, extensively, extremely, or totally drug resistant, coupled with the decreasing efficacies of conventional therapies, necessitates the development of novel treatments. As viruses that infect Mycobacterium spp., mycobacteriophages may represent a strategy to combat and eradicate drug-resistant TB. More exploration is needed to provide a comprehensive understanding of mycobacteriophages and their genome structure, which could pave the way toward a definitive treatment for TB. This review focuses on the properties of mycobacteriophages, their potential in diagnosing and treating TB, the benefits and drawbacks of their application, and their use in human health. Specifically, we summarize recent research on mycobacteriophages targeted against Mtb infection and newly developed mycobacteriophage-based tools to diagnose and treat diseases caused by Mycobacterium spp. We underscore the urgent need for innovative approaches and highlight the potential of mycobacteriophages as a promising avenue for developing effective diagnosis and treatment to combat drug-resistant Mycobacterium strains.","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141971186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbiological insights and dermatological applications of live biotherapeutic products. 活体生物治疗产品的微生物学见解和皮肤病应用。

IF 3.2 3区生物学

Journal of Applied Microbiology Pub Date : 2024-08-05 DOI: 10.1093/jambio/lxae181

Jessica Locker, Hannah J Serrage, Ruth G Ledder, Shivprasad Deshmukh, Catherine A O'Neill, Andrew J McBain

{"title":"Microbiological insights and dermatological applications of live biotherapeutic products.","authors":"Jessica Locker, Hannah J Serrage, Ruth G Ledder, Shivprasad Deshmukh, Catherine A O'Neill, Andrew J McBain","doi":"10.1093/jambio/lxae181","DOIUrl":"10.1093/jambio/lxae181","url":null,"abstract":"As our understanding of dermatological conditions advances, it becomes increasingly evident that traditional pharmaceutical interventions are not universally effective. The intricate balance of the skin microbiota plays a pivotal role in the development of various skin conditions, prompting a growing interest in probiotics, or live biotherapeutic products (LBPs), as potential remedies. Specifically, the topical application of LBPs to modulate bacterial populations on the skin has emerged as a promising approach to alleviate symptoms associated with common skin conditions. This review considers LBPs and their application in addressing a wide spectrum of dermatological conditions with particular emphasis on three key areas: acne, atopic dermatitis, and wound healing. Within this context, the critical role of strain selection is presented as a pivotal factor in effectively managing these dermatological concerns. Additionally, the review considers formulation challenges associated with probiotic viability and proposes a personalised approach to facilitate compatibility with the skin's unique microenvironment. This analysis offers valuable insights into the potential of LBPs in dermatological applications, underlining their promise in reshaping the landscape of dermatological treatments while acknowledging the hurdles that must be overcome to unlock their full potential.","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141874940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0