Journal of Applied Microbiology最新文献

{"title":"Pyrogallol Exhibits Antimicrobial Adjuvant Properties Against Staphylococcus aureus and Staphylococcus epidermidis.","authors":"Katharina H C Roese, Sarah Ibrahim, Lauren A Cooper, Afnan S Yahya, Dongjae Kang, Nikhil R Tirupathigari, Koby A J LaRose, Karen L Houseknecht, Deborah J Barlow, Kristin M Burkholder","doi":"10.1093/jambio/lxaf189","DOIUrl":"https://doi.org/10.1093/jambio/lxaf189","url":null,"abstract":"<p><strong>Aims: </strong>To investigate the effect of pyrogallol as an antimicrobial adjuvant against staphylococci.</p><p><strong>Methods and results: </strong>We tested the effect of pyrogallol on the susceptibility of four strains of S. aureus and S. epidermidis to antibiotics. In minimum inhibitory concentration (MIC) assays, pyrogallol significantly enhanced the anti-staphylococcal efficacy of linezolid against all strains and increased cefoxitin and oxacillin activity against individual strains. Given pyrogallol's prooxidant properties, we compared its effect on linezolid activity in wild-type and catalase-deficient S. aureus. Catalase-deficient bacteria exhibited greater linezolid sensitivity than WT in presence of pyrogallol. Antibiotic accumulation assays revealed greater intracellular linezolid concentration in S. aureus treated with pyrogallol compared to bacteria treated with linezolid alone.</p><p><strong>Conclusions: </strong>Pyrogallol potentiates linezolid-mediated killing of staphylococci in a manner involving oxidative stress and intra-bacterial antibiotic accumulation. Pyrogallol may have utility as an anti-staphylococcal antimicrobial adjuvant.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144731104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of the GPCR FfGpr1-Gα-AC Signal Transduction in Fusarium fujikuroi and Its Role in Gibberellin Biosynthesis.","authors":"Yu-Ke Cen, Min-Han Li, Jiang-Tao Li, Jing-Wen Jia, Qi Wang, Xu-Sheng Liu, Yuan-Shan Wang, Ya-Ping Xue, Zhi-Qiang Liu, Yu-Guo Zheng","doi":"10.1093/jambio/lxaf191","DOIUrl":"https://doi.org/10.1093/jambio/lxaf191","url":null,"abstract":"<p><strong>Aims: </strong>This study aimed to characterize the GPCR FfGpr1-Gα-AC transduction pathway and its role in regulating gibberellin (GA) metabolism in Fusarium fujikuroi.</p><p><strong>Methods and results: </strong>By constructing Ffgpr1 deletion and constitutively activated FfG2Q204 L mutants, we found that glucose-induced cAMP synthesis was abolished in Ffgpr1Δ. Bimolecular fluorescence complementation confirmed membrane-localized FfGpr1-FfG2 and FfG2-AC interactions, with intensified fluorescence at septa. Fermentation assays revealed opposing GA3 yields: Ffgpr1Δ produced 21% less GA3 than the wild type, whereas FfG2Q204 L increased yield by 17%. qPCR analysis demonstrated that Ffgpr1Δ upregulated FfCPS/KS, FfP450-2, and FfP450-3 transcription by 6-8-fold while downregulating FfDES by 82%, whereas FfG2Q204 L induced a 6-fold increase in FfCPS/KS mRNA level. Strikingly, FfDES overexpression in Ffgpr1Δ restored GA3 production to wild-type levels but led to GA7 accumulation and suppressed FfP450-3 upregulation, suggesting feedback-regulated metabolic constraints.</p><p><strong>Conclusion: </strong>Glucose-induced cAMP production required FfGpr1. FfGpr1-FfG2 and FfG2-AC interacted on the cell membrane, with enhanced co-localization at the septal region. The FfGpr1-Gα-AC pathway significantly affected GA yield, with complex and noteworthy regulation of GA cluster gene expression.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144731101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic and virulence insights of Western European Aeromonas salmonicida subsp. salmonicida and development of Galleria mellonella infection assay.","authors":"Salomé Desmecht, Fabrice Touzain, Thibaut Olivier, Céline Antoine, Véronique Beven, Cécile Meex, François Lieffrig, Steve J Charette, Damien Thiry","doi":"10.1093/jambio/lxaf193","DOIUrl":"https://doi.org/10.1093/jambio/lxaf193","url":null,"abstract":"<p><strong>Aims: </strong>Aeromonas salmonicida subsp. salmonicida is the etiological agent of furunculosis, a fish disease highly aggressive for salmonids and responsible for significant economic losses in aquaculture worldwide. This study aimed to explore genomic and antimicrobial resistance traits of Western European A. salmonicida subsp. salmonicida strains and to develop an adapted infection model using larvae of the greater wax moth Galleria mellonella to assess the pathogenic potential of this psychrophilic subspecies.</p><p><strong>Methods and results: </strong>Three A. salmonicida subsp. salmonicida strains, isolated from salmonids displaying clinical signs of furunculosis, were tested against a panel of antibiotics and sequenced to characterize their genome. Virulence was evaluated in G. mellonella larvae using bacterial doses ranging from 101 to 106 CFU/larva. Two isolates exhibited multidrug resistance to antibiotics commonly used against furunculosis. Although closely related to the reference strain A449, genomic analyses revealed multiple plasmids known to encode antibiotic resistance genes. Virulence assays showed that this subspecies was lethal at doses as low as 101 CFU/larva, and that a fully functional Type III secretion system (T3SS) is not essential for the infection of G. mellonella, likely due to the presence of other virulence factors in T3SS-deficient strains.</p><p><strong>Conclusions: </strong>These findings enhance the genomic characterization of European A. salmonicida subsp. salmonicida and validate the use of Galleria mellonella larvae as a relevant alternative infection model for studying this psychrophilic subspecies.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144731103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An investigation of the microbiological quality of smoked fish in England and detection of Listeria monocytogenes strains associated with listeriosis.","authors":"F Jorgensen, M Kesby, C Amar, D Rajendram, C Barker, A Crewdson, A Painset, L Sadler Reeves, H Aird, S Lai, C Willis","doi":"10.1093/jambio/lxaf192","DOIUrl":"https://doi.org/10.1093/jambio/lxaf192","url":null,"abstract":"<p><strong>Aims: </strong>A survey to determine the presence of Listeria and indicator bacteria in smoked fish was performed from 2022 to 2023.</p><p><strong>Methods and results: </strong>L. monocytogenes was detected in 3.6% of 783 samples. L. monocytogenes was detected more frequently in cold smoked (4.9%) compared to hot smoked (2.0%) samples. The majority (63%) of samples had a water activity that would not prevent the growth of L. monocytogenes. Elevated levels of Enterobacteriaceae (> 10 000 CFU g-1) were detected in 4.7% of samples. L. monocytogenes was detected more frequently in samples with elevated levels of Enterobacteriaceae. Isolates of L. monocytogenes were compared using whole genome sequencing to historic isolates. Isolates from three samples were the same strain as that associated with a clonal complex (CC) 217 outbreak under investigation and detected despite an earlier product recall. Twelve isolates were CC121 strains and 10 of these belonged to six Single Nucleotide Polymorphism single-linkage clusters with one strain linked to a human case from 2021. One CC415 strain was also linked to a human case. The remaining isolates belonged to CC8, CC9, CC14, CC37, CC31, CC398, CC87 and CC475 with no links to human cases. Investigation of historical isolates revealed that 11 strains were associated with specific manufacturers for up to 10 years. The presence of quaternary ammonium compound efflux genes showed an association with CC.</p><p><strong>Conclusions: </strong>Diverse L. monocytogenes detected in smoked fish with persistent strains associated with some manufacturing premises. Correlation between presence of L. monocytogenes and high levels of Enterobacteriaceae was detected.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144731100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic analyses of Escherichia coli ST410 harbouring the blaOXA-181 carbapenemase gene from companion animals and humans in Switzerland.","authors":"Yvonne Spahr, Patrice Nordmann, Javier E Fernandez, Laurent Poirel, Andrea Endimiani, Vincent Perreten","doi":"10.1093/jambio/lxaf181","DOIUrl":"https://doi.org/10.1093/jambio/lxaf181","url":null,"abstract":"<p><strong>Aims: </strong>To determine the genomic relatedness of E. coli (Ec) sequence type (ST) 410 producing the carbapenemase OXA-181 (ST410-OXA-181) which caused an outbreak in a Swiss companion animal (CA) clinic in 2018 with those isolated from humans between 2017 and 2021 in Switzerland, and to characterize complete plasmids harbouring antimicrobial resistance genes (ARGs).</p><p><strong>Methods and results: </strong>The complete genomes of 31 Ec ST410-OXA-181 (8 from CA, 23 from humans) were obtained by hybrid assembly of reads from Illumina and Nanopore sequencing technologies. The genomes were used for in silico phylogenetic analysis (cgMLST, SNP, ST410 clade analysis), ARG screening and for comparative analysis of complete plasmids. Antimicrobial susceptibility was determined by MIC measurement. All veterinary strains (CA, veterinarian) belonged to the phylogenetic clade ST410-B2 and the human strains to either ST410-B2 or B3. Strains recovered from the veterinary setting were clonal, differing by 0-4 SNPs. A higher genetic distance (>131 SNPs) was observed between the veterinary and the clinical human strains. All veterinary and 15 human strains shared an identical IncX3 plasmid harbouring blaOXA-181. Additional multidrug resistance plasmids were detected in human strains only.</p><p><strong>Conclusions: </strong>The Ec ST410-B2-OXA-181 lineage was detected in both humans and animals. They share the same OXA-181 plasmids but differed by SNP-based genetic distances and ARG contents indicating broad dissemination potential and independent evolution of strains of this lineage.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144731102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic relatedness and antimicrobial resistance profiles of β-lactamase-producing Escherichia coli from environmental and animal sources in West Texas: A One Health approach.","authors":"Yamima Tasnim, Md Kaisar Rahman, Babafela Awosile","doi":"10.1093/jambio/lxaf190","DOIUrl":"https://doi.org/10.1093/jambio/lxaf190","url":null,"abstract":"<p><strong>Aims: </strong>We aimed to determine the genetic relatedness among β-lactamase-producing Escherichia coli isolated from soil, lake water, and feces of geese, pigs, dogs, cattle, coyotes, wild hogs, and horses at one health interface in West Texas.</p><p><strong>Methods and results: </strong>Previously isolated 56 β-lactamase-producing E. coli isolates from the feces of geese, pigs, horses, coyotes, dogs, cattle, and wild hogs, and soil and lake water from different locations in West Texas were analyzed for genetic relatedness using whole-genome sequencing, core genome multilocus sequence typing, and phylogenetic single-nucleotide polymorphism (SNP) analysis using the publicly available bioinformatic platforms. Among 56 β-lactamase-producing E. coli isolates, 82.1% carried blaCTX-M genes, 42.86% carried blaTEM genes, 7.14% carried blaOXA-10, and 3.57% contained blaCMY-2 genes. 9 different types of phylogroups, 35 serotypes and 36 sequence types, 32 distinct plasmid groups, 75 types of virulence genes, and 46 core genome multilocus sequence types were identified. Using 100 maximum of SNPs between the isolates to define a clone, 14 clones were identified in 13 E. coli isolates from animal feces and environmental samples. The clonally related E. coli isolates were clustered based on the phylogenetic tree.</p><p><strong>Conclusion: </strong>The findings from this study provide limited evidence of clonal dissemination of β-lactamase-producing E. coli between different animal sources.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144707562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction and metabolomics of silage-derived lactic acid bacteria-based consortia.","authors":"Cheng Zong, Longxin Wang, Jie Zhao, Zhihao Dong, Junfeng Li, Xianjun Yuan, Chengti Xu, Tao Shao","doi":"10.1093/jambio/lxaf178","DOIUrl":"https://doi.org/10.1093/jambio/lxaf178","url":null,"abstract":"<p><strong>Aims: </strong>The objective of this study was to construct and analyze the metabolomics of silage-derived lactic acid bacteria (LAB)-based consortia.</p><p><strong>Methods and results: </strong>The LAB strains were isolated from various silages, and LAB-based consortia with different types of biodiversity were constructed by a bottom-up approach. Two hundred and ninety-two LAB strains were isolated, nine of which were selected as representative strains. Subsequently, five LAB consortia with diverse biodiversity levels (ranging from 4 to 8 species) were constructed. This construction was based on two key factors: the absence of antagonism among strains and their acidification capabilities, which were characterized by pH values (3.60-3.71), lactate (23.6-26.4 g L-1), and acetate (6.68-9.04 g L-1). Further fermentation experiments demonstrated that the five LAB consortia exhibited superior performance compared to alfalfa fermented green juice. Untargeted metabolomics was employed to analyze the differentially abundant metabolites and metabolic pathways of LAB consortia. Notably, 6 amino acids and 5 B-group vitamins varied among five LAB consortia, and key metabolic pathways were related to amino acids and vitamins for the LAB consortia.</p><p><strong>Conclusions: </strong>Five LAB consortia with high acidification ability were constructed. The primary metabolic differences observed among the LAB consortia were the levels of 6 amino acids and 5 B-group vitamins.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144690359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cultivation of marine chitinolytic bacteria reveals the sponge-associated Motilimonas isolate Spo1_1 as an efficient degrader of insoluble chitin.","authors":"Etienne Dechamps, Arthur Salengros, Laurence Meunier, Séverine Chevalier, Johan Danguy, Sophie-Luise Heidig, Jean-François Flot, Tina Keller-Costa, Rodrigo Costa, Isabelle F George","doi":"10.1093/jambio/lxaf187","DOIUrl":"https://doi.org/10.1093/jambio/lxaf187","url":null,"abstract":"<p><strong>Aims: </strong>Culture-independent studies in the literature suggest that marine habitats hold diversified chitin-degrading microbial communities. This study was conducted to isolate novel chitinolytic bacteria from two bacteria-rich marine biotopes, sponges and sediments, and compare the efficiency with which those strains degrade different forms of chitin.</p><p><strong>Methods and results: </strong>Bacterial colonies were isolated from chitinolytic consortia derived from the microbiota of the marine sponge Hymeniacidon perlevis of and its surrounding sediment collected at Audresselles beach, France. Many isolates (49%) produced a halo of chitin degradation on colloidal chitin agar plates, including isolates belonging to two genera (Motilimonas, Pseudophaeobacter) yet unknown as chitin degraders. However, 83% of the positive isolates degraded poorly insoluble chitin powder in liquid cultures. Nine isolates were further tested for colloidal chitin degradation in liquid cultures and exhibited contrasting results. One isolate, Motilimonas Spo1_1, exhibited the strongest chitinolytic activity in liquid culture continaing insoluble chitin powder (i.e. 37% of degradation). The analysis of its genome and that of other Motilimonas spp. revealed an arsenal of genes for chitin degradation. Genomic analyses suggest that Spo1_1 is a new species within the genus Motilimonas, we propose the name Motilimonas chitinovorans.</p><p><strong>Conclusions: </strong>Motilimonas Spo1_1 largely outperformed all 70 other strains in terms of its insoluble chitin degradation capabilities, including strains belonging to the well-known chitinolytic genera Vibrio and Pseudoalteromonas. Those results encourage further studies on the potential of Motilimonas spp. to eliminate chitinous waste. More generally, they confirm that marine habitats are a reservoir of chitinolytic microbes yet to be discovered.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144690360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sludge-to-gas: Aquaculture fish sludge shows varying hydrogen production potential over the lifetime of a fish.","authors":"Biwen Annie An-Stepec, Melanie Andrews, Abduljelil Kedir, Natalie Schindler, Nicole Dopffel","doi":"10.1093/jambio/lxaf188","DOIUrl":"https://doi.org/10.1093/jambio/lxaf188","url":null,"abstract":"<p><strong>Aims: </strong>The fish industry is growing, with millions of tons of fish cultured in off-land and on-land aquaculture systems. A major challenge is the continuous production of sludge containing fish secretions and uneaten fish feed. Anaerobic digestion (ad) can efficiently use this sludge, involving biological degradation of organic matter by microbes under anoxic conditions, forming valuable biogases like methane. Recently, there's an increasing need for green hydrogen (H2), but the potential of using fish sludge for H2 production hasn't been fully explored. A key complicating factor is the changing sludge composition during a fish's lifetime.</p><p><strong>Methods and results: </strong>Fish sludge was sampled from salmon in a land-based recirculating aquaculture system (RAS) over six months, covering fresh-, brackish-, and seawater stages. Sludge was used for ad batch incubation to investigate H2 and liquid compound productivity. Chemical- and DNA-based microbial community analysis of the sludge showed significant changes over time. This was also reflected in changing ad productivity. Highest H2 production (24.5 ± 17.5 mL H2 gas per 1-gram dry sludge) was within the freshwater phase, followed by brackish. Concurrently in all enrichments we observed high production of CO2 (75.3 ± 30.1 mL CO2 per 1-gram of dry sludge) and volatile fatty acids especially acetic acid, propionic acid, and lactic acid.</p><p><strong>Conclusions: </strong>The microbial community in fish sludge can naturally produce H2 and other valuable products, with productivity varying over the fish's lifetime. The key governing factor for H2 production is the type of fish sludge and availability of electron acceptors.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144690362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lactic acid bacteria strains isolated from rehydrated corn grain silage enhance the chemical and microbiological characteristics and aerobic stability of these silages.","authors":"Yasmim A Silva, Marcus V S B Cardoso, Beatriz F Carvalho, Rosane F Schwan, Carla L S Ávila","doi":"10.1093/jambio/lxaf185","DOIUrl":"https://doi.org/10.1093/jambio/lxaf185","url":null,"abstract":"<p><strong>Aims: </strong>This study aimed to isolate, characterize, and select lactic acid bacteria (LAB) strains from rehydrated corn grain silages (RCGSs) and evaluated the effects of these strains as inoculants for improving silage chemical composition, microbiological quality, and aerobic stability.</p><p><strong>Methods and results: </strong>One hundred and forty LAB were isolated from RCGSs, characterized, and subjected to growth and pH reduction tests in corn grain extract. Thirty-nine strains were evaluated for metabolite production, starch degradation, and inhibitory activity against Escherichia coli and Bacillus cereus. Selected strains (21) included Pediococcus acidilactici (PA), P. pentosaceus (PP), Lactiplantibacillus plantarum (LP); and Limosilactobacillus fermentum (LF). After 60 days of storage, strains 180 (PA) and 190 (PP) showed the greatest pH reduction (3.69) and lactic acid production (4.72%) but lower aerobic stability (AS) (27 h). The AS was highest in silages inoculated with strains 185 (PP) and 126 (PA) (92.3 h). Inoculation with strains 221 (PP) and 116 (LF) (1.95%) had the lowest dry matter (DM) losses (1.95%), while strains 77, 79 (PA); 190, 110, and 52 (PP) minimized starch losses (8.22%).</p><p><strong>Conclusion: </strong>RCGS epiphytic strains can improve silage fermentation profiles, each strain contributing specific benefits based on its metabolism.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144690361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}