Journal of Applied Microbiology最新文献

{"title":"Reverse vaccinology and host-pathogen analysis reveal pagP as a potential universal vaccine candidate against avian pathogenic Escherichia coli in poultry.","authors":"Linan Jia, Xin Ye, Mark A Arick, Chuan-Yu Hsu, Jeffrey D Evans, Kelsy Robinson, Pratima Adhikari, Li Zhang","doi":"10.1093/jambio/lxaf253","DOIUrl":"https://doi.org/10.1093/jambio/lxaf253","url":null,"abstract":"<p><strong>Aims: </strong>Avian pathogenic Escherichia coli (APEC) causes colibacillosis, requiring effective vaccines to prevent this devastating disease in poultry. Current strain-specific vaccines show limited protection against diverse APEC strains, highlighting the need to identify conserved antigens for broad-protective vaccine development. This study combines reverse vaccinology (RV) analysis of APEC whole genome sequences with host-pathogen interaction studies to identify universal vaccine candidates, their expression, and effect on host immune responses. These complementary approaches aim to identify and select potential antigens for the development of broad-spectrum vaccines against APEC infections in poultry.</p><p><strong>Methods and results: </strong>A total of 3,061 core genome protein coding sequences were predicted from 25 APEC isolates, including 44 proteins that met the criteria of subcellular localization (extracellular and outer membrane), number of transmembrane helix (0 or 1), and adhesion probability score (≥ 0.5). Among them, 32 proteins were antigenic (antigen score ≥ 0.5) and highly conserved across the E. coli database. Finally, 13 vaccine candidates were predicted to have the immunogenicity potential. During the host-pathogen interaction studies, selected E. coli isolates induced a host immune reaction (all with P ≤ 0.01), and among the 13 predicted vaccine candidates, pagP showed a highly upregulated expression level during the interaction (P ≤ 0.01).</p><p><strong>Conclusions: </strong>In this study, pagP was identified as having the most potential for future development of recombinant vaccines against APEC.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145251205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salmonella in the slaughter sheep population in England and Wales.","authors":"Susan E Lewis, Shaun A Cawthraw, Andrew D Wales, Meg Rawlins, Daniel Brown, Isaac Ring, Tom Huby, Cam Sandu, Rachel Jinks, Lucy C Snow, Steve Wyllie, Adrienne Mackintosh, Richard P Smith, Sarah Evans, Francesca Martelli","doi":"10.1093/jambio/lxaf244","DOIUrl":"https://doi.org/10.1093/jambio/lxaf244","url":null,"abstract":"<p><strong>Aims: </strong>A survey of Salmonella in sheep caecal content samples was undertaken in England and Wales in 2023.</p><p><strong>Methods: </strong>Over nine months, repeated sampling was conducted in 12 abattoirs that represented 28% of national annual sheep throughput with good geographical coverage. Samples of caecal content were obtained from 1228 sheep at abattoirs and cultured for Salmonella spp.</p><p><strong>Results: </strong>Salmonella was isolated from 104 (8.5%) samples, with 21 isolations (1.7%) of S. Typhimurium. The most commonly isolated serovar (80 samples, 6.5%) was the sheep-adapted Salmonella enterica subspecies diarizonae serovar 61: k:1,5,(7), which has low zoonotic potential. Modelling estimated the mean annual Salmonella prevalence to be 10.5% (95% CI 8.5-13.0%). Multivariate logistic regression indicated that the likelihood of Salmonella isolation was significantly associated with animal age, with the odds of a sheep over 12 months old yielding Salmonella being significantly greater than for those under 12 months (OR 7.2, p < 0.001). For serovars of public health importance, particularly S. Typhimurium, analysis was limited by the small number of isolations, but univariate analysis indicated a significant association between over-12-month animals and the likelihood of isolating S. Typhimurium, similar to overall Salmonella isolation risk.</p><p><strong>Conclusions: </strong>Our study has assessed Salmonella carriage among sheep presented for slaughter and intended for the food chain and found 8.5% of samples positive. It provides an improved understanding of the prevalence, distribution and subtypes of Salmonella in slaughter sheep of different ages indicating that older sheep were more often associated with isolation of Salmonella.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145251219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Niche differentiation of anaerobic ammonium-oxidizing bacteria and denitrifying bacteria in artificial and natural water-level fluctuation zones of the three gorges reservoir area.","authors":"Yiying Wu, Yufei Bao, Yuchun Wang, Shuang Liu, Shanze Li, Jie Wen, Aiqin Wang, Hanxiao He, Jianwei Zhao","doi":"10.1093/jambio/lxaf251","DOIUrl":"https://doi.org/10.1093/jambio/lxaf251","url":null,"abstract":"<p><strong>Aims: </strong>The water-level fluctuation zone (WLFZ) serves as a critical ecotone for nitrogen cycling in reservoirs, but microbial differentiation between artificial and natural WLFZs remains unclear. This study aims to explore how WLFZ type (artificial vs. natural) and hydrological gradients influence the niche differentiation of anaerobic ammonium-oxidizing (Anammox) and denitrifying bacteria.</p><p><strong>Methods and results: </strong>Using soil physicochemical analysis, qPCR, and high-throughput sequencing, we investigated the communities of Anammox and denitrifying bacteria in artificial and natural WLFZs at four elevation levels (150-180 m) in the Three Gorges Reservoir area. The results revealed that artificial WLFZ had significantly greater abundance and diversity of Anammox bacteria and nirS/nirK-type denitrifiers than natural WLFZ. The abundance of Anammox increased with increasing water level. Moreover, the diversity and abundance of nirS-type denitrifiers were generally higher than those of nirK-type denitrifiers, indicating better adaptation of nirS-type denitrifiers to the WLFZ environment in the Three Gorges Reservoir Area. Anammox and denitrifiers predominantly exhibited competitive ecological interactions driven by NO₂⁻ dynamics. In contrast, nirS/nirK-type denitrifiers were influenced by the C/N ratio and moisture content and could coexist in the WLFZ.</p><p><strong>Conclusions: </strong>WLFZ type and water level jointly shape the structure and ecological relationships of nitrogen-transforming bacteria. Artificial WLFZ provides more favorable conditions for nitrogen cycling. These findings offer insights into microbial nitrogen dynamics and inform reservoir management strategies.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145251224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic combination of imipenem and 7-fluoroindole inhibits β-lactamases and biofilm in Carbapenem-resistant Acinetobacter baumannii.","authors":"Anandita Chalana, Vipasha Thakur, Varsha Gupta, Prince Sharma, Neena Capalash","doi":"10.1093/jambio/lxaf234","DOIUrl":"10.1093/jambio/lxaf234","url":null,"abstract":"<p><strong>Background: </strong>Acinetobacter baumannii is a nosocomial opportunistic pathogen responsible for hospital and community-acquired infections. It has been categorised as a high-priority ESKAPE pathogen due to escalating resistance, underscoring the urgent need for effective treatment options. This study explores the use of 7-Fluoroindole (7-FI) as an adjuvant to imipenem for combating Carbapenem-Resistant Acinetobacter baumannii (CRAB).</p><p><strong>Methods: </strong>Clinical strains of A. baumannii were subjected to disc diffusion assay for antibiotic resistance profile, and Fractional Inhibitory Concentration (FIC) of 7-FI was determined with imipenem by checkerboard assay. Expression of β-lactamases, efflux pumps, and outer membrane proteins was determined by qRT-PCR. Biofilm inhibition by 7-FI in combination with imipenem was evaluated by Confocal Laser Scanning Microscopy.</p><p><strong>Results: </strong>7-FI reduced the MIC of imipenem by 2 to 8-fold in CRAB strains, and also enhanced susceptibility to meropenem. Mechanistically, 7-FI inhibited the β-lactamase activity by 2.3 to 3.8-fold and downregulated the expression of Class B (blaNDM-1) and D (blaOXA-23 and blaOXA-51) β-lactamases. The efflux pump activity was reduced by 2 to 8.6-fold, and expression of efflux pump genes adeB, adeJ, abeM and emrA was also significantly (p < 0.001) downregulated. 7-FI in combination with imipenem inhibited biofilm formation by 80% even at 1 × FIC (1/4 MIC of imipenem and 1/3 MIC of 7-FI).</p><p><strong>Conclusion: </strong>The combination of 7-FI and imipenem synergistically mitigated carbapenem resistance in A. baumannii by inhibiting the activity of β-lactamases, downregulating the expression of β-lactamases and efflux pumps. The study shows 7-FI as a useful adjuvant to imipenem against CRAB strains.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145113052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the therapeutic role of probiotics and prebiotics in mitigating diseases associated with advanced glycation end products.","authors":"Haiqing Sun, Zhenjiang Liu, Xin Li, Dongxu Wen, Bin Li, Xiaodan Huang","doi":"10.1093/jambio/lxaf233","DOIUrl":"10.1093/jambio/lxaf233","url":null,"abstract":"<p><p>Advanced glycation end products (AGEs) are a class of compounds generated when reducing sugars interact with proteins, lipids, and other molecular structures. Excessive accumulation of AGEs in the body accelerates aging and contributes to various chronic conditions, including diabetes, neurodegenerative diseases, cardiovascular diseases, and inflammatory bowel diseases. Despite growing interest in strategies to prevent glycation damage, effective interventions to combat AGE-related diseases remain limited. This review examines the role of AGEs in disease pathology and evaluates strategies to mitigate their harmful effects. It further summarizes natural products and pharmaceutical agents currently used for anti-glycation purposes, along with their underlying mechanisms. Additionally, we explore the promising therapeutic potential of probiotics and prebiotics in protecting against AGE-related diseases, highlighting research evidence demonstrating their ability to reduce AGE accumulation and inhibit their formation. This review aims to inform the development of more effective glycation inhibitors and addresses the pressing gap in current research regarding targeted AGEs interventions.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145091839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Same-day multiplex detection of foodborne bacterial pathogens for clinical diagnostics and food safety.","authors":"Pei-Yi Tsui, Fang-Tzy Wu, Yi-Ling Chen, Chu-Yang Chien, Feng-Ping Lin, Chung-Chih Liang, Hsin-Hsien Huang, Chin-Mao Hung, Hsiao-Lun Wei, Shih-Hua Teng, Shan-Ko Tsai, Ching-Yi Wu, Hui-Ling Hsu","doi":"10.1093/jambio/lxaf225","DOIUrl":"10.1093/jambio/lxaf225","url":null,"abstract":"<p><strong>Aims: </strong>This study aims to develop and evaluate a rapid and high-multiplex pathogen detection method for clinical and food specimens to address the ongoing public health threat of foodborne infections and the limitations of conventional culture-based diagnostics.</p><p><strong>Methods and results: </strong>The foodborne bacteria (FBB) assay integrates multiplex PCR, T7 exonuclease hydrolysis, and a suspension bead array to simultaneously detect 16 genes from 13 major foodborne bacteria. Analytical performance was evaluated using reference strains, while diagnostic performance was assessed using clinical and food samples. The FBB assay demonstrated high specificity and sensitivity, with minimum detectable amounts ranging from 5 to 100 copies per reaction for all targets except one. Among 106 clinical specimens from foodborne outbreaks, the assay achieved 99.1-100% overall % agreement with routine methods. In spiked food samples, Bacillus cereus was detected at 1 CFU g-1 in cooked rice, and Listeria monocytogenes at 10² CFU mL-1 in milk; for the latter, sensitivity improved to 10 CFU mL-1 and 1 CFU mL-1 after four and 16 hours of pre-enrichment, respectively.</p><p><strong>Conclusions: </strong>The FBB assay enables culture-independent, multiplex detection of foodborne bacterial pathogens within six hours and demonstrates robust analytical and diagnostic performance.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145015493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel Erythromycin Lipid Nanoformulations for Aquaculture Applications.","authors":"Sarah Chagas Campanharo, Gabriela Alberto Gil, Agnaldo Fernando Baldo da Silva, Inácio Mateus Assane, Daniel de Abreu Reis Ferreira, Fabiana Pilarski, James Jacob Sasanya, Wanderley Pereira de Oliveira, Jonas Augusto Rizzato Paschoal","doi":"10.1093/jambio/lxaf249","DOIUrl":"https://doi.org/10.1093/jambio/lxaf249","url":null,"abstract":"<p><strong>Aims: </strong>This study introduces novel lipid nanoformulations of erythromycin (ERY) designed for aquaculture pathogens and evaluates their in vitro antimicrobial activity compared to pure ERY.</p><p><strong>Methods and results: </strong>Among the tested formulations, the nanoemulsion (F4) demonstrated higher inhibitory effects. The MIC values were 25 µg mL⁻¹ against Aeromonas hydrophila and A. caviae, and for A. veronii, all ERY-loaded nanoformulations displayed high activity (MIC ≤ 3.1 µg mL⁻¹), as determined by the broth microdilution method. Lipid nanoformulations were prepared using a hot emulsification method. The method consisted of high-speed homogenization at 22 000 rpm for 5 minutes using an Ultra-Turrax homogenizer, followed by ultrasonication. The surfactants used included poloxamer and natural surfactants, quillaja (Quillaja saponaria Molina) saponins and whey protein concentrate. The nanoformulations were characterized by particle size (228-500 nm), polydispersity (0.189-0.456), zeta potential (-42 to -27.5 mV), pH (4.54-5.5), conductivity (161-295 µS cm-1), and stability.</p><p><strong>Conclusions: </strong>The nanoemulsion (F4) kept higher ERY content at early time points and showed equal or lower MICs than free ERY against selected Aeromonas spp. The dispersions can be spray-dried to a powder or incorporated into fish feed by extrusion or post-pellet coating-routes rarely reported for ERY in aquaculture. These results support further development of ERY nanoformulations against aquaculture pathogens.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145232541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Linezolid resistance in immunocompromised patients: Co-occurrence of optrA and fexA genes harbored by a novel plasmid in Enterocococus faecalis ST300.","authors":"Anis Raddaoui, Yosra Chebbi, Siwar Frigui, Wafa Achour","doi":"10.1093/jambio/lxaf248","DOIUrl":"https://doi.org/10.1093/jambio/lxaf248","url":null,"abstract":"<p><strong>Aims: </strong>Linezolid resistance in Gram-positive cocci has been reported in clinical settings but it is still a rare phenomenon. This study investigated the molecular epidemiology of linezolid-resistant Enterococcus faecalis strains and the genetic environment of linezolid-resistance genes, in immunocompromised patients.</p><p><strong>Methods and results: </strong>This study investigated two linezolid-resistant Enterococcus faecalis strains (Minimal inhibitory concentration = 16 mg/L). These colonizing strains were isolated from patients at Tunisia's National Bone Marrow Transplant Center. Both strains exhibited a multidrug resistant phenotype. Whole-genome sequencing revealed the co-occurrence of optrA and fexA resistance genes, for the first time, in ST300 and ST861 Enterococcus faecalis. Concerning the ST300 strain, optrA and fexA were located on a novel plasmid pS1987-optrA sharing high similarity with plasmids from clinical isolates in Japan and the Netherlands. Comparative genomics highlighted genetic diversity in the optrA region among geographically linked isolates, suggesting plasmid-mediated spread.</p><p><strong>Conclusion: </strong>This study reported, for the first time, the co-occurrence of optrA and fexA genes in linezolid-resistant Enterococcus faecalis ST300 and ST861, harbored by a novel plasmid in the ST300 strain.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145232524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low water activity limits bentonite-associated microbial growth.","authors":"Rhiannon Punch, Lucas Beckering Vinckers Stofer, C M James Neurauter, Rachel C Beaver, Katja Engel, Simcha Stroes-Gascoyne, Chang Seok Kim, W Jeffrey Binns, Myrna J Simpson, Greg F Slater, Josh D Neufeld","doi":"10.1093/jambio/lxaf140","DOIUrl":"10.1093/jambio/lxaf140","url":null,"abstract":"<p><strong>Aims: </strong>This research investigated the impact of water activity on microbial abundance estimates from bentonite under conditions relevant to a deep geological repository for used nuclear fuel. Because previous research tested saturation of bentonite within pressurized vessels, the goal of this study was to assess how water activity alone, without pressure, prevents increases in microbial abundance estimates from bentonite samples.</p><p><strong>Methods and results: </strong>Small-scale microcosms of bentonite were hydrated to low, medium, or high water activities, with Type I water, reference groundwater, or bacterial growth medium, then incubated under oxic or anoxic conditions. At six timepoints over a 6-month period, microorganisms were quantified using cultivation-dependent and independent approaches, and 16S rRNA genes were sequenced to monitor relative abundance changes of microbial taxa. Large-scale incubations were then conducted to also enable analyses of phospholipid fatty acids and natural organic matter. The results demonstrate that increasing water activity was associated with higher microbial abundance estimates for oxic condition incubations, with water-activity-dependent actinobacterial growth. In contrast, no significant microbial abundance changes were observed for anoxic microcosm incubations. For all tested conditions, we did not detect any increases in sulfate-reducing bacteria abundance estimates.</p><p><strong>Conclusions: </strong>Although low water activity conditions prevented changes in microbial abundances for bentonite samples incubated under oxic conditions, anoxic conditions alone prevented increases in abundances of culturable microorganisms. These results complement previous pressure-vessel studies that have shown how low water activity and elevated pressure simultaneously reduce the abundance of viable microorganisms that can initially proliferate during the saturation process.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144215830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut dysbiosis allows foodborne salmonella colonization in edible crickets: a probiotic strategy for enhanced food safety.","authors":"Shuma Tsuji, Osamu Matsushita, Jumpei Uchiyama, Kenji Yokota, Tetsuya Bando, Hideyo Ohuchi, Kazuyoshi Gotoh","doi":"10.1093/jambio/lxaf217","DOIUrl":"10.1093/jambio/lxaf217","url":null,"abstract":"<p><strong>Aims: </strong>Edible insects, including crickets, represent a promising protein source, yet concerns over foodborne pathogens limit consumer acceptance. This study investigated whether gut microbiota modulates colonization by Salmonella enterica subsp. enterica serovar Enteritidis (SE) in the two-spotted cricket (Gryllus bimaculatus).</p><p><strong>Methods and results: </strong>Under standard conditions, SE was undetectable in crickets despite prolonged exposure; however, antibiotic-induced dysbiosis enabled stable SE colonization. Long-read 16S rRNA sequencing revealed significant microbiota shifts, notably a reduction in Lactococcus garvieae. In vitro assays showed strong inhibitory effects of L. garvieae against SE, and supplementation of dysbiotic crickets with L. garvieae reduced SE colonization by ∼1000-fold.</p><p><strong>Conclusions: </strong>The native cricket gut microbiota, especially L. garvieae, plays a protective role against SE colonization. Enhancing beneficial gut bacteria could mitigate pathogen risks and promote edible insects as a sustainable protein.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144955282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}