Journal of Applied Microbiology最新文献

Efficacy of photoClO2 against two human norovirus surrogates and Clostridioides difficile endospores on stainless steel and nylon carpet. 光二氧化氯对不锈钢和尼龙地毯上两种人类诺如病毒代用品和艰难梭菌内孢子的功效。

IF 3.2 3区生物学

Journal of Applied Microbiology Pub Date : 2024-11-23 DOI: 10.1093/jambio/lxae294

Jinge Huang, Jacob Adams, Charles Pettigrew, Angela Fraser, Xiuping Jiang

{"title":"Efficacy of photoClO2 against two human norovirus surrogates and Clostridioides difficile endospores on stainless steel and nylon carpet.","authors":"Jinge Huang, Jacob Adams, Charles Pettigrew, Angela Fraser, Xiuping Jiang","doi":"10.1093/jambio/lxae294","DOIUrl":"https://doi.org/10.1093/jambio/lxae294","url":null,"abstract":"Aims: Determine efficacy of an aqueous photocatalytic disinfection system, photoClO2, against two human norovirus surrogates [feline calicivirus (FCV) and Tulane virus (TuV)] and Clostridioides difficile endospores on stainless steel and nylon carpet.Methods and results: The photoClO2 system was first optimized with 1% sodium chlorite (NaClO2) and 10 ppm Eosin Y to produce 60.64 ppm ClO2/min in a 4.5×4.5 cm2 area. It was then tested against FCV, TuV, and C. difficile endospores on stainless steel and nylon carpet with two different backings. On stainless steel, photoClO2 achieved a >5 log10 plaque-forming unit (PFU) reduction of FCV in 45 min, >3 log10 median tissue culture infectious dose (TCID50) reduction of TuV in 60 min, and 1.3 log10 colony-forming unit (CFU) reduction of C. difficile endospores in 120 min. Under indoor lighting conditions, photoClO2 achieved a 4.3 log10 PFU reduction of FCV and 1.4 log10 TCID50 reduction of TuV on stainless steel after 120 min. Further, photoClO2 achieved a 2.9 log10 PFU reduction of FCV and 2.5 log10 TCID50 reduction of TuV on nylon carpet with waterproof backing in 60 min, which was higher than carpet with water-permeable backing (1.3 log10 PFU and 1.1 log10 TCID50 reduction, respectively).Conclusion: ClO2 production rate of the photoClO2 system was influenced by light distribution, while disinfection efficacy was affected by light intensity, surface characteristics, and target microorganisms. PhotoClO2 was efficacious in inactivating both human norovirus surrogates on stainless steel and nylon carpet. Efficacy against C. difficile endospores was limited.","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142695565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of feeding black soldier fly (Hermetia illucens), cricket (Gryllodes sigillatus), and superworm (Zophobas morio) on the gut microbiota of rainbow trout (Oncorhynchus mykiss). 喂食黑兵蝇（Hermetia illucens）、蟋蟀（Gryllodes sigillatus）和超级蠕虫（Zophobas morio）对虹鳟（Oncorhynchus mykiss）肠道微生物群的影响。

IF 3.2 3区生物学

Journal of Applied Microbiology Pub Date : 2024-11-23 DOI: 10.1093/jambio/lxae295

Sonja Drosdowech, Samantha Bezner, Brendan Daisley, Marcia Chiasson, Anne Easton, Neil Rooney, David Huyben

{"title":"Influence of feeding black soldier fly (Hermetia illucens), cricket (Gryllodes sigillatus), and superworm (Zophobas morio) on the gut microbiota of rainbow trout (Oncorhynchus mykiss).","authors":"Sonja Drosdowech, Samantha Bezner, Brendan Daisley, Marcia Chiasson, Anne Easton, Neil Rooney, David Huyben","doi":"10.1093/jambio/lxae295","DOIUrl":"https://doi.org/10.1093/jambio/lxae295","url":null,"abstract":"Aim: This study investigates how replacing fishmeal and fish oil with insect meals in feed impacts the gut microbiota in rainbow trout (Oncorhynchus mykiss), a crucial species in aquaculture.Methods and results: Dietary inclusion of black soldier fly (Hermetia illucens), cricket (Gryllodes sigillatus), and superworm (Zophobas morio) were evaluated for their impact on intestinal microbial diversity and community composition following a 12-week feeding trial. Fish were fed one of four isonitrogenous and isoenergetic diets: a control diet without insect meal, and diets with 15% defatted black soldier fly meal, full-fat adult cricket meal, or full-fat superworm meal. The microbiota of intestinal digesta and fish feed was characterized using 16S rRNA gene sequencing on the Illumina MiSeq platform. Results revealed significantly lower alpha diversity indices in the cricket treatment compared to the control. Beta diversity analysis showed Bacillota as the dominant phylum across all treatments, with the initial stock population richer in Mycoplasmatota. A novel genus within Mycoplasmataceae was prevalent at day 0 and in all treatments. Black soldier fly meal increased an unidentified Peptostreptococcaceae genus (bsv123) compared to control and superworm diets, while cricket meal elevated Streptococcus levels.Conclusions: Insect-based diets, particularly with black soldier fly meal, significantly alter beta diversity within the gut microbiota of rainbow trout, with cricket meal reducing alpha diversity and superworm having minimal impact.","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142695566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Occurrence of Salmonella enterica in faecal sludge from Nigeria and genetic relatedness with strains associated with human infections in Africa. 尼日利亚粪便污泥中肠炎沙门氏菌的出现以及与非洲人类感染相关菌株的遗传相关性。

IF 3.2 3区生物学

Journal of Applied Microbiology Pub Date : 2024-11-22 DOI: 10.1093/jambio/lxae293

Abdurrahman Hassan Jibril, Anders Dalsgaard, Iruka N Okeke, Aliyu Ibrahim Musawa, John Elmerdahl Olsen

{"title":"Occurrence of Salmonella enterica in faecal sludge from Nigeria and genetic relatedness with strains associated with human infections in Africa.","authors":"Abdurrahman Hassan Jibril, Anders Dalsgaard, Iruka N Okeke, Aliyu Ibrahim Musawa, John Elmerdahl Olsen","doi":"10.1093/jambio/lxae293","DOIUrl":"https://doi.org/10.1093/jambio/lxae293","url":null,"abstract":"Aim: This study investigated occurrence of Salmonella in faecal sludge from public toilets in Nigeria, and genetic relatedness of strains which have been reported to cause human infection across Africa.Methods and results: The study collected 150 human sludge from public toilets, and identified Salmonella through culture and PCR. Isolates were tested for antimicrobial susceptibility and sequenced using Illumina MiSeq. Draft sequences were compared with sequence data from Enterobase and Genbank. Twenty-four (16.0%) of sewage samples were positive for Salmonella [CI95 (10.2- 21.8)]. S. Give (sequence type (ST) 516), S. Seftenberg (ST-14), and S. Chester (ST-411) were the most prevalent serovars found in 45.8%, 16.7%, and 16.7% of samples, respectively. Most of the isolates were sensitive to the antimicrobials tested, only one isolate of S. Derby showed resistance to ampicillin and cefazolin. Notably, 91.7% of the strains had the aac (6)-Iaa gene, and point mutations in parC, gyrA and acrB. S. Chester showed genetic relatedness with strains from Benin Republic and South Africa.Conclusion: There is genetic relatedness of present strains and those associated with human infections in Africa.","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142692913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epidemiology and pathogen characteristics of infections following solid organ transplantation. 实体器官移植后感染的流行病学和病原体特征。

IF 3.2 3区生物学

Journal of Applied Microbiology Pub Date : 2024-11-20 DOI: 10.1093/jambio/lxae292

Fanjie Meng, Chi Zhu, Chan Zhu, Jiaxuan Sun, Dongsheng Chen, Ran Ding, Liyuan Cui

引用次数: 0

Glucose-1-phosphate thymidylyltransferase promotes the production of 3-O-α-mycarosylerythronolide B in Streptomyces coelicolor. 葡萄糖-1-磷酸胸苷基转移酶促进共生链霉菌产生 3-O-α-mycarosylerythronolide B。

IF 3.2 3区生物学

Journal of Applied Microbiology Pub Date : 2024-11-18 DOI: 10.1093/jambio/lxae291

Hong Gao, Swen Langer, Tony Larson, Matthew A Gregory, Margaret C M Smith

引用次数: 0

Harnessing bacterial antimicrobial peptides: a comprehensive review on properties, mechanisms, applications, and challenges in combating antimicrobial resistance. 利用细菌抗菌肽：全面回顾抗菌肽的特性、机制、应用以及在对抗抗菌药耐药性方面所面临的挑战。

IF 3.2 3区生物学

Journal of Applied Microbiology Pub Date : 2024-11-18 DOI: 10.1093/jambio/

Pirasannah Erriah, Sheau Ling Puan, Normi Mohd Yahaya, Wan Nur Ismah Wan Ahmad Kamil, Syafinaz Amin Nordin, Azira Muhamad, Suriana Sabri

引用次数: 0

Inoculation with PGPR co-fermented broths promotes growth, yields and quality of pepper by increasing soil nutrients and changing soil bacterial community. 通过增加土壤养分和改变土壤细菌群落，接种 PGPR 共同发酵肉汤可促进辣椒的生长、产量和质量。

IF 3.2 3区生物学

Journal of Applied Microbiology Pub Date : 2024-11-15 DOI: 10.1093/jambio/lxae289

Yujie Han, Lei Tian, Yu Luo, Lizhen Han

{"title":"Inoculation with PGPR co-fermented broths promotes growth, yields and quality of pepper by increasing soil nutrients and changing soil bacterial community.","authors":"Yujie Han, Lei Tian, Yu Luo, Lizhen Han","doi":"10.1093/jambio/lxae289","DOIUrl":"https://doi.org/10.1093/jambio/lxae289","url":null,"abstract":"Aims: The purpose of this study was to explore impacts of different combinations of co-fermentation PGPR broth on the growth and yield of pepper.Methods and results: The effects of co-fermentation broth containing a random combination of two PGPR strains on the growth of pepper were analyzed. All inoculation treatments promoted growth, yield and quality of peppers. The relative abundance of the dominant Proteobacteria and Sphingomonas was significantly higher in the planted soil at the seedling and fruiting stages, and the soil available nitrogen, phosphorus, and potassium contents were correspondingly higher. In addition, the co-fermented broth of Bacillus velezensis HP9 and Burkholderia pyrrocinia P10 treatment had the most pronounced effect on plant growth, while the combination of Bacillus flexus HGD12 and P10 had the greatest impact on fruit nutritional indices. This is consistent with the highest enrichment of beneficial bacterial genera at the seedling stage in the HP9 and P10 treatment, and at the fruiting stage in the P10 and HGD12 treatment group, respectively.Conclusions: Different combinations of co-fermented bacterial broths increased soil nutrient contents and changed the bacterial community, which in turn promoted the growth, yield and quality of pepper.","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142638979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

How to validate UV-C based air cleaners using viruses containing aerosols in a test room. 如何在试验室中使用含有病毒的气溶胶验证基于紫外线-C 的空气净化器。

IF 3.2 3区生物学

Journal of Applied Microbiology Pub Date : 2024-11-14 DOI: 10.1093/jambio/lxae287

B Kramer, D Warschat, A Meepool, P Muranyi

{"title":"How to validate UV-C based air cleaners using viruses containing aerosols in a test room.","authors":"B Kramer, D Warschat, A Meepool, P Muranyi","doi":"10.1093/jambio/lxae287","DOIUrl":"10.1093/jambio/lxae287","url":null,"abstract":"Aims: UV-C based air cleaners may reduce the transmission of infectious diseases. However, microbiological validation is necessary to quantify their efficiency. In this study, the stability of aerosolized bacteriophages for validation purposes was investigated in a test room, before an UV-C based air cleaner was exemplarily evaluated regarding the inactivation of airborne bacteriophages.Methods and results: The bacteriophage Phi6 was selected as virus surrogate and aerosolized in a room of 30 m³ volume. The recovery of infectious bacteriophages was first analyzed under variation of the relative humidity (20-55% RH) and sampling time. The aerosol studies showed that a low humidity between 20% RH and 30% RH provides a high and stable recovery of bacteriophages Phi6 over 1 h. However, with increasing humidity, the number of infectious airborne bacteriophages Phi6 decreased significantly. At 50% RH, the recovery of Phi6 was 4 orders of magnitude lower compared to 20% RH. The validation of an UV-C based air cleaner was then demonstrated in the test room whereat the decline of infectious airborne bacteriophages was recorded over time. The non-enveloped bacteriophage MS2 was used as a reference. The validation results were significantly different for Phi6 when the humidity in the test room was either 40% RH or 30% RH whereas comparable results were obtained for MS2 at both humidities.Conclusion: A rising humidity in the test room caused a significant decline in the recovery of infectious airborne bacteriophages Phi6. The result of a quantitative validation of UV-C based air cleaners may therefore be affected by the respective humidity.","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142621001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of a cellulose-aminating polysaccharide from a filamentous sulfur-oxidizing bacterium, Thiothrix nivea, grown lithotrophically or mixotrophically. 从一种丝状硫氧化细菌 Thiothrix nivea 中生产纤维素氨基多糖，该细菌以石养或混养方式生长。

IF 3.2 3区生物学

Journal of Applied Microbiology Pub Date : 2024-11-14 DOI: 10.1093/jambio/lxae288

Tomoaki Saito, Yunkun Qiao, Yui Araki, Naoki Matsunaga, Wataru Osugi, Keiko Kondo, Masato Katahira, Minoru Takeda

{"title":"Production of a cellulose-aminating polysaccharide from a filamentous sulfur-oxidizing bacterium, Thiothrix nivea, grown lithotrophically or mixotrophically.","authors":"Tomoaki Saito, Yunkun Qiao, Yui Araki, Naoki Matsunaga, Wataru Osugi, Keiko Kondo, Masato Katahira, Minoru Takeda","doi":"10.1093/jambio/lxae288","DOIUrl":"10.1093/jambio/lxae288","url":null,"abstract":"Aims: Glucosaminoglucan (β-1,4-linked glucose and glucosamine) produced by a mixotrophic sulfur-oxidizing bacterium, Thiothrix nivea, is a useful cellulose-aminating agent. Lithotrophic and mixotrophic glucosaminoglucan production were examined using fed-batch techniques.Methods and results: A jar fermenter was used for the fed-batch cultivation. Glucosaminoglucan was extracted from T. nivea using diluted HCl. Lithotrophic growth was detected by feeding with Na2S as the energy source, and 12 mg L-culture-1 of glucosaminoglucan was obtained. In contrast, no growth was observed with Na2S2O3. Similarly, mixotrophic growth in the presence of acetic acid was promoted by Na2S, whereas Na2S2O3 had no effect. When acetic acid and Na2S were added, 470 mg L-culture-1 of glucosaminoglucan was obtained.Conclusions: T. nivea was cultured and glucosaminoglucan was produced lithotrophically using Na2S for feeding. Na2S is also indispensable for mixotrophic growth and glucosaminoglucan production, indicating that sulfide oxidation pathways control the TCA cycle. The involvement of the SOX pathway (for thiosulfate oxidation) in the activation of energy metabolism is doubtful because neither lithotrophic nor mixotrophic growth was promoted by Na2S2O3. Based on these results, we assumed that T. nivea is facultatively mixotrophic (lithotrophic growth is possible in addition to organotrophic growth in the presence of sulfide (Na2S)), rather than obligately mixotrophic.","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142621018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A single upstream mutation of whiB7 underlies amikacin and clarithromycin resistance in Mycobacterium abscessus. whiB7的单个上游突变是脓肿分枝杆菌产生阿米卡星和克拉霉素耐药性的基础。

IF 3.2 3区生物学

Journal of Applied Microbiology Pub Date : 2024-11-13 DOI: 10.1093/jambio/lxae286

Nathan De Boeck, Cristina Villellas, Estefanía Crespo-Yuste, Jesús Gonzalo-Asensio, Peter T Buckley, Kim Thys, Cuong Vuong, Nacer Lounis, Natalie Verstraeten, Jan Michiels

{"title":"A single upstream mutation of whiB7 underlies amikacin and clarithromycin resistance in Mycobacterium abscessus.","authors":"Nathan De Boeck, Cristina Villellas, Estefanía Crespo-Yuste, Jesús Gonzalo-Asensio, Peter T Buckley, Kim Thys, Cuong Vuong, Nacer Lounis, Natalie Verstraeten, Jan Michiels","doi":"10.1093/jambio/lxae286","DOIUrl":"https://doi.org/10.1093/jambio/lxae286","url":null,"abstract":"Aims: We aimed to investigate the molecular mechanisms underlying the survival of Mycobacterium abscessus when faced with antibiotic combination therapy. By conducting evolution experiments and whole genome sequencing (WGS), we sought to identify genetic variants associated with stress response mechanisms, with a particular focus on drug survival and resistance.Methods and results: We conducted evolution experiments on M. abscessus, exposing the bacteria to a combination therapy of amikacin and rifabutin. Genetic mutations associated with increased antibiotic survival and altered susceptibility were subsequently identified by WGS. We focused on mutations that contribute to stress response mechanisms and tolerance. Of particular interest was a novel frameshift mutation in MAB_3509c, a gene of unknown function within the upstream open reading frame of whiB7. A MAB_3509c knockout mutant was constructed, and expression of downstream drug resistance genes was assessed by RT-qPCR. Mutation of MAB_3509c results in increased RNA levels of whiB7 and downstream stress response genes such as eis2, which is responsible for aminoglycoside resistance.Conclusion: Our findings demonstrate the importance of whiB7 in the adaptive stress response in M. abscessus. Moreover, our results highlight the complexity of M. abscessus adapting to drug stress and underscore the need for further research.","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142620997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0