Journal of Applied Microbiology最新文献

{"title":"Lactic acid bacteria strains isolated from rehydrated corn grain silage enhance the chemical and microbiological characteristics and aerobic stability of these silages.","authors":"Yasmim A Silva, Marcus V S B Cardoso, Beatriz F Carvalho, Rosane F Schwan, Carla L S Ávila","doi":"10.1093/jambio/lxaf185","DOIUrl":"https://doi.org/10.1093/jambio/lxaf185","url":null,"abstract":"<p><strong>Aims: </strong>This study aimed to isolate, characterize, and select lactic acid bacteria (LAB) strains from rehydrated corn grain silages (RCGSs) and evaluated the effects of these strains as inoculants for improving silage chemical composition, microbiological quality, and aerobic stability.</p><p><strong>Methods and results: </strong>One hundred and forty LAB were isolated from RCGSs, characterized, and subjected to growth and pH reduction tests in corn grain extract. Thirty-nine strains were evaluated for metabolite production, starch degradation, and inhibitory activity against Escherichia coli and Bacillus cereus. Selected strains (21) included Pediococcus acidilactici (PA), P. pentosaceus (PP), Lactiplantibacillus plantarum (LP); and Limosilactobacillus fermentum (LF). After 60 days of storage, strains 180 (PA) and 190 (PP) showed the greatest pH reduction (3.69) and lactic acid production (4.72%) but lower aerobic stability (AS) (27 h). The AS was highest in silages inoculated with strains 185 (PP) and 126 (PA) (92.3 h). Inoculation with strains 221 (PP) and 116 (LF) (1.95%) had the lowest dry matter (DM) losses (1.95%), while strains 77, 79 (PA); 190, 110, and 52 (PP) minimized starch losses (8.22%).</p><p><strong>Conclusion: </strong>RCGS epiphytic strains can improve silage fermentation profiles, each strain contributing specific benefits based on its metabolism.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144690361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sanguinarine Hydrogel Accelerates Wound Healing in Staphylococcus pseudintermedius Infections by Suppressing Biofilm Formation.","authors":"Jintong Liu, Jiahui Wu, Ting Zheng, Di Zhang, Jiahao Lin","doi":"10.1093/jambio/lxaf184","DOIUrl":"https://doi.org/10.1093/jambio/lxaf184","url":null,"abstract":"<p><strong>Aims: </strong>Skin and ear infections are common in small animals, primarily caused by the opportunistic pathogen Staphylococcus pseudintermedius. S. pseudintermedius biofilms are associated with poor prognosis, and rising antibiotic resistance threatens public health, making the development of new antibacterial agents imperative. Sanguinarine (SAN) is a plant-derived alkaloid with a wide range of pharmacological activities, including antibacterial, anti-inflammatory, and antitumor effects. The antibacterial potential of SAN against S. pseudintermedius merits further investigation.</p><p><strong>Methods and results: </strong>The MIC and MBC of SAN hydrogel against clinically isolated S. pseudintermedius were determined as 1.25 mg mL-1 and 5 mg mL-1, respectively. Phenol-sulfuric acid and XTT assays demonstrated that SAN hydrogel significantly delayed biofilm formation (P < 0.05) by suppressing exopolysaccharide (EPS) synthesis and biofilm metabolic activity. Live/dead fluorescent staining and scanning electron microscopy (SEM) confirmed its potent disruptive effects on mature biofilms, inducing bacterial lysis and death, while reducing biofilm thickness and extracellular matrix. In a murine skin infection model, SAN hydrogel (2.5/5 mg mL-1) exhibited significant antibacterial efficacy (P < 0.001), accelerated wound healing, and reduced bacterial load (P < 0.001) and IL-6 levels (P < 0.0001) in skin tissues. No significant hepatorenal toxicity was observed within the tested concentration range.</p><p><strong>Conclusion: </strong>The SAN hydrogel demonstrates antimicrobial and bactericidal activities against S. pseudintermedius, effectively suppressing biofilm formation and holding promise as a therapeutic candidate for skin infections caused by this pathogen.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144674881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the Antimicrobial Activity of Gut Catabolites of Quercetin 4'-glucoside on Listeria monocytogenes and Their Effects on Membrane Integrity and Biofilm Formation.","authors":"Yujia Liu, Yue Gao, Conghui Shi, Xiaoyang Liu","doi":"10.1093/jambio/lxaf183","DOIUrl":"https://doi.org/10.1093/jambio/lxaf183","url":null,"abstract":"<p><strong>Aims: </strong>Listeria (L.) monocytogenes is the well-known foodborne pathogen that requires critical control in the human body. 3-hydroxyphenylacetic acid (OPAC) and 3,4-dihydroxyphenylacetic acid are gut catabolites of quercetin 4'-glucoside in onion, their antibacterial effects would help to control the risk of L. monocytogenes. Here, we investigate their antibacterial activity, action at the transcript level and effects on membrane and biofilm to better control L. monocytogenes.</p><p><strong>Methods and results: </strong>RNA-Seq data revealed that the OPAC and DOPAC treatments to L. monocytogenes led to the identification of enormously differently expressed genes in cellular components (membrane) and phosphotransferase system pathway at their 1/2 MIC (7.805 mM⸱L-1). DOPAC exhibited more obviously damaged in membrane (max 33.94%) and biofilm (max 88.2%) than that of OPAC which may be related to their different effects on cell hydrophobicity, mobility and morphology.</p><p><strong>Conclusions: </strong>These findings provided a credibly inhibitory mechanism of OPAC and DOPAC on L. monocytogenes, offering the theoretical basis for potential applications on the other kinds of phenolic acids that would possess similar structures in food industry.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144659248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Large-scale analysis of polyhydroxyalkanoate synthases in Pseudomonas: highly diverse enzymes with potential for a novel class and dissemination by horizontal gene transfer.","authors":"Amelia Cox-Fermandois, Camilo Berríos-Pastén, Carlos Serrano, Patricio Arros, Ignacio Poblete-Castro, Andrés Marcoleta","doi":"10.1093/jambio/lxaf179","DOIUrl":"https://doi.org/10.1093/jambio/lxaf179","url":null,"abstract":"<p><strong>Aims: </strong>To investigate the diversity, phylogenetic distribution, and structural features of polyhydroxyalkanoate (PHA) synthases (PhaCs), key enzymes for producing bioplastics, in different well-known and poorly-studied species of Pseudomonas. As Antarctic Pseudomonas spp. with unique PhaCs and PHA synthesis capabilities have been reported, we aimed to explore the PhaC dotation and classes in strains from this and other environments and the dissemination potential of the phaC genes.</p><p><strong>Methods and results: </strong>We compared 859 genomes from 186 Pseudomonas species, including 33 from Antarctica. PhaC gene identification, multiple alignments, phylogenetic inference, and 3D structure prediction were applied to compare and classify the PhaCs. Most isolates encoded two class II PhaCs, some showing additional class II and class I enzymes, especially from Antarctica, outstanding P. frigusceleri MPC6 harboring five PhaCs, one from a potential novel class. Different PhaC subclasses were proposed based on this diversity. Despite substantive sequence variation, all the PhaCs showed a highly conserved 3D structure. Also, several phaC genes were inside putative genomic islands, phages, and plasmids, supporting their acquisition by multiple horizontal transfer routes.</p><p><strong>Conclusions: </strong>To our knowledge, this is the first report investigating the PhaCs present across the Pseudomonas genus, unveiling a remarkable diversity of these enzymes and their common dissemination in mobile elements, likely contributing to the host cell fitness. Our findings underline the potential of Pseudomonads from Antarctica and other environments and their PhaCs for producing PHAs with varying monomer compositions and properties. Future research is essential to elucidate the enzymatic properties of this underexplored PhaC diversity.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144649517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolism of Prebiotic Oligosaccharides by Lactic Acid Bacteria Isolated From Rabbits: Insights into Strain-Specific Utilization and Applications.","authors":"Guizhen Gong, Angmu Duojie, Yurong He, Jianxia Yang, Xinrui Zhang, Yang Chen, Yuanting Zhu","doi":"10.1093/jambio/lxaf182","DOIUrl":"https://doi.org/10.1093/jambio/lxaf182","url":null,"abstract":"<p><strong>Aims: </strong>To investigate the in vitro utilization of functional oligosaccharides by lactic acid bacteria (LAB) isolated from rabbits, characterize their metabolic kinetics and genomic determinants, and evaluate their in vitro probiotic traits when grown on oligosaccharides.</p><p><strong>Methods and results: </strong>Ninety-two LAB strains from weaned rabbits were screened for utilization of isomaltose- (IMO), galactose- (GOS), chitosan- (COS), and mannose-oligosaccharides (MOS). Strain- and substrate-specific utilization patterns were observed: 63% utilized IMO, 68.5% GOS, 16.3% MOS, and none metabolized COS. Lacticaseibacillus paracasei YT170-selected for its broad substrate range-showed distinct growth kinetics and pH dynamics on IMO, GOS, and MOS. Genomic analysis revealed specialized carbohydrate-active enzymes (CAZymes) for each oligosaccharide. After 72 h fermentation by YT170, cell-free supernatants (CFS) from three oligosaccharides exhibited enhanced antioxidant activities (DPPH scavenging > 88%) and substrate-dependent antimicrobial effects: MOS fermentation most effectively inhibited Escherichia coli (ZOI: 21.30 ± 0.66 mm vs. LGG 19.33 ± 0.29 mm), while GOS fermentation showed maximal activity against Staphylococcus aureus (ZOI: 25.17 ± 0.95 mm).</p><p><strong>Conclusion: </strong>Oligosaccharide utilization by LAB from rabbits is strain- and substrate-specific, and L. paracasei YT170 demonstrates metabolic versatility, enabling synbiotic design with IMO, GOS, or MOS to modulate gut microbiota in weaned rabbits, but further in vivo studies are required.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144649518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mucoid phenotype in clinical isolates of Escherichia coli: an underexplored phenomenon.","authors":"Atanu Manna, Dhamodharan Ramasamy, K Vanathy, S Srirangaraj, R Ramya, Divya Lakshmanan","doi":"10.1093/jambio/lxaf166","DOIUrl":"10.1093/jambio/lxaf166","url":null,"abstract":"<p><p>Escherichia coli is an important clinical pathogen that requires urgent attention. Although a commensal bacterium, E. coli can cause serious infections ranging from enterohaemorrhagic diarrhoea to neonatal meningitis. The pathogenesis of E. coli is characterized by a complex interplay of virulence factors that facilitate its colonization, invasion, and evasion of host immune responses. The adaptive capability of the pathogen to varying host environment is facilitated by its genetic plasticity leading to patho-adaptive mutations. Production of mucoid capsule represents a way of such phenotypic adaptation. Mucoid phenotype is commonly found and associated with enhanced virulence in Klebsiella pneumoniae, Pseudomonas aeruginosa, and Acinetobacter baumannii. However, mucoid colony types are not common in clinical E. coli isolates and their correlation to virulence, biofilm formation and antibiotic resistance is not thoroughly studied. A majority of studies are based on laboratory-generated mutants, while information on clinical mucoid strains is limited. Hence, understanding the challenges posed by mucoid E. coli in clinical settings might play a crucial role in treatment and infection control measures. This review compiles information on the phenotypic characteristics, virulence determinants, and associated diseases of mucoid E. coli, and aims to address the gaps in knowledge for a better understanding of its clinical implications.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144583964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interface-associated microbial growth in anoxic pressure vessels containing compacted gapfill bentonite.","authors":"Rachel C Beaver, Claire S Tully, W Jeffrey Binns, James J Noël, Josh D Neufeld","doi":"10.1093/jambio/lxaf157","DOIUrl":"10.1093/jambio/lxaf157","url":null,"abstract":"<p><strong>Aims: </strong>Deep geological repositories (DGR) for belowground storage of used nuclear fuel rely on highly compacted bentonite to limit growth of microorganisms, including those that can contribute to microbiologically influenced corrosion, like sulfate-reducing bacteria (SRB). This study examined how a range of bentonite dry densities (1.25-1.60 g cm-3) affected microbial community abundance and composition in bentonite incubated in pressure vessels for 1, 4, or 12 months under anoxic conditions.</p><p><strong>Results: </strong>Culturable heterotroph abundance increased in some lower-density (i.e., 1.25 and 1.40 g cm-3) samples after 1 month, consistent with previous experiments. Unlike previous studies, outer layer bentonite samples had higher abundances of culturable SRB than the starting material. SRB-associated sequences were more prevalent in swabs from pressure vessel surfaces than in bentonite, suggesting interface-associated SRB growth.</p><p><strong>Conclusions: </strong>Although culturable SRB increased for several outer layer pressure vessel samples, abundances remained relatively low and did not increase beyond the values measured at the 1-month timepoint. Overall, a minimum dry density of 1.45 g cm-3 limited microbial growth in inner layer bentonite samples.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144505781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serological detection and molecular typing of Mycobacterium avium subspecies paratuberculosis in sheep and goats co-infected with gastrointestinal parasites from the northern Himalayan territories of India.","authors":"Misbah Altaf, Shaheen Farooq, Mohd Altaf Bhat, Zahid Amin Kashoo, Padma Yangzom, Uznain Tramboo, Sabia Qureshi, Isfaq- Ul-Hussain, Idrees Mehraj Allaie","doi":"10.1093/jambio/lxaf180","DOIUrl":"10.1093/jambio/lxaf180","url":null,"abstract":"<p><strong>Aim: </strong>The present study was conducted to investigate the occurrence of paratuberculosis infection and gastrointestinal (GI) parasitism in small ruminants, including the threatened Changthangi goats of the Ladakh region and to characterize the Map strain diversity and detection of anti-Mycobacterium avium subspecies paratuberculosis (Map) antibodies in serum samples of sheep and goats.</p><p><strong>Methods and results: </strong>A total of 327 faecal samples were collected from 32 flocks across various locations in the Kashmir and Ladakh regions and were examined for the presence of Map bacilli. Initial screening using Ziehl-Neelsen staining detected acid-fast bacilli in 111 samples (33.9%), of which 88 were confirmed positive for Map by IS900 and IS1311 Polymerase chain reaction (PCR) assays. The overall occurrence of Map infection in sheep and goats was 26.9%. Molecular typing of the 88 PCR-positive samples using IS1311 PCR-restriction enzyme analysis identified the B-type Map in 85 samples (96.5%) while 3 samples (3.4%) belonged to S-type strain; no C-type strains were detected in this study. Sequencing of 13 representative IS1311 amplicons (B-type = 10; S-type = 3) demonstrated that all B-type sequences possessed intact Thymine & Guanine nucleotides at positions 64 and 65, consistent with the US Bison type, while the S-type sequences also showed complete homology, confirming single bison and sheep biotypes circulating in the study population. Additionally, the faecal analysis indicated a high occurrence (85.32%) of GI parasites in small ruminants with Haemonchus spp. being the most prevalent (79.0%), followed by Trichostrongylus spp. (12%). Furthermore, among 150 serum samples collected from 20 flocks in the Kashmir region, enzyme-linked immunosorbent assay (ELISA) detected anti-Map antibodies in 39 (26.0%) samples. At the flock level, 65% were seropositive, while individual-level seroprevalence was 18.0% in sheep and 42.0% in goats.</p><p><strong>Conclusion: </strong>The study reveals an overall Map infection rate of 26.9% in small ruminants, with the B-type strain as the dominant biotype, exhibiting sequence identity with the US Bison type. ELISA-based seroprevalence was 26.0%, while GI parasitism was widespread, affecting over 85% of animals, with Haemonchus spp. as the predominant parasite affecting sheep and goats.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144649562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acid or salt adaptation of Listeria monocytogenes 10403S grown until exponential phase aerobically, enhances sensitivity to oxidative stress.","authors":"Marcia Boura, Mahide M Yilmaz Topcam, David Spiteri, Carolina Bruschi, Vasileios Valdramidis, Kimon A G Karatzas","doi":"10.1093/jambio/lxaf173","DOIUrl":"10.1093/jambio/lxaf173","url":null,"abstract":"<p><strong>Aims: </strong>The work aimed at investigating a possible role of sigB in catalase transcription and activity in Listeria monocytogenes. Furthermore, we also aimed to investigate whether sigB upregulation during the exponential phase, due to acid or salt adaptation, could result in hypersensitivity to oxidative stress. Finally, we investigated how this discovery could be used in the wider concept of Hurdle Technology through combination of different stresses.</p><p><strong>Methods and results: </strong>Listeria monocytogenes 10403S WT and ΔsigB strains were grown aerobically, and catalase transcription and activity were assessed at different growth stages. Catalase transcription peaked at 6 h of growth in both strains, with ΔsigB showing higher levels. Subsequently, from 8 to 10 h, a major drop to similarly low levels occurred for both strains. However, catalase activity peaked 2 h later (at 8 h of growth) than transcription and remained higher in ΔsigB beyond this point. To evaluate stress adaptation, exponential-phase cells were exposed to sub-lethal acidic conditions (pH 4.5; HCl) or salt (0.5 mol l-1 NaCl) and later subjected to H2O2 or sonication (tested only with acid). Adaptation increased sensitivity in the wild type (WT) but not in ΔsigB, underpinning the negative role of sigB upregulation. Acid adaptation reduced catalase activity in both strains, explaining the reduced oxidative stress resistance, although salt adaptation did not affect catalase activity. After adaptation to acid or salt, application of oxidative stress without removing the initial adaptation stresses resulted in a higher synergistic effect in both WT and ΔsigB.</p><p><strong>Conclusion: </strong>The above synergistic effects are important for our understanding of listerial oxidative stress resistance and optimization of relevant oxidative stress decontamination processes (e.g. oxidative compounds, ultrasound, and plasma treatments) but also virulence.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144626403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diversity of optrA genetic structures in Enterococcus faecalis from retail chicken and the differential biofilm-forming abilities of isolates at non-frozen temperatures.","authors":"Yan Zhang, Yexin Lin, Jing Zhang, Ruanyang Sun, Jintao Yang, Mark A Webber, Hongxia Jiang, Chao Zhuo","doi":"10.1093/jambio/lxaf148","DOIUrl":"10.1093/jambio/lxaf148","url":null,"abstract":"<p><strong>Aims: </strong>This study aimed to explore the diversity of optrA genetic structures in Enterococcus faecalis from retail chicken and the variance in their biofilm-forming capabilities at refrigerated and room temperatures. By comprehensively studying these two traits, we aim to fully understand the transmission and biofilm-forming capabilities at non-frozen temperatures of optrA-positive E. faecalis.</p><p><strong>Methods and results: </strong>Analysis of Chinese-sourced E. faecalis genomes in the database indicated that 65.76% were optrA-positive, with animals as the predominant hosts. In 2020, 15 optrA-carrying strains were isolated from 96 retail chicken samples from four Chinese supermarkets. The optrA gene was situated on plasmids or chromosomes. A novel optrA-carrying plasmid structure was identified, resulting from IS1485-mediated structural changes. Tn6674-like and Tn558-like structures mediated inter-chromosomal transfer of optrA, while IS1216E and ISEnfa1 promoted inter-plasmid transfer. Some isolates showed a greater tendency for biofilm formation at refrigerated temperature, and others at room temperature.</p><p><strong>Conclusions: </strong>In conclusion, this research reveals the genetic intricacy of optrA-positive E. faecalis and their temperature-associated biofilm-forming behaviors, highlighting the necessity of monitoring food-related microbial hazards.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144284456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}