Journal of Applied Microbiology最新文献

{"title":"The effects of introduced biocontrol microbes on root and flower surface microbiomes of strawberry grown in coir substrate in a polythene tunnel.","authors":"Xiangming Xu, Greg Deakin, Georgina Fagg, Tom Passey","doi":"10.1093/jambio/lxaf074","DOIUrl":"10.1093/jambio/lxaf074","url":null,"abstract":"<p><strong>Aims: </strong>Biocontrol of plant diseases is a key component of sustainable agriculture. Augmented introduction of biocontrol microbes may affect the resident phytobiome, potentially leading to unintended consequences. We studied the effects of three biocontrol microbes (Gliocladium catenulatum, Trichoderma asperellum, and Bacillus subtilis) on communities of living microbes on table-top strawberries grown in coir bags in a polythene tunnel.</p><p><strong>Methods and results: </strong>Gliocladium catenulatum and T. asperellum were applied to roots via drenching soon after planting, and G. catenulatum and B. subtilis were sprayed onto flowers. To profile communities of living microbes, we treated samples with PMAxx™ before DNA extraction. Flower and root epiphytes were profiled by amplicon and shot-gun sequencing, respectively. Applying the three microbes led to significant increases in their relative abundance on both flowers and roots. The two introduced fungal taxa (G. catenulatum and T. asperellum) appeared to affect mainly fungal microbiome components, whereas the introduced bacterial taxon (B. subtilis) mainly affected bacterial microbiome components. The effects of introduced biocontrol microbes on the resident microbiome were more profound and long-lasting on roots than on flowers.</p><p><strong>Conclusions: </strong>Introduced microbes can persist better on roots than on flowers. Although the applied microbes led to significant changes in the overall microbial composition, their effects on individual taxa groups were limited. Introduced microbes appeared to have only largely affected microbial communities in the same kingdom.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143719282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surface sanitation against foodborne pathogens in domestic environments: efficacy of a handheld ultraviolet C light emitting diode device.","authors":"Francesco Palma, Giulia Baldelli, Giulia Amagliani, Asja Conti, Mauro De Santi, Giorgio Brandi, Giuditta Fiorella Schiavano","doi":"10.1093/jambio/lxaf072","DOIUrl":"10.1093/jambio/lxaf072","url":null,"abstract":"<p><strong>Aim: </strong>Ensuring food safety is fundamental to protect public health, especially in domestic environments where food is handled daily. This two-phase study aimed to investigate the efficacy of an ultraviolet-C light emitting diode (UV-C LED) handheld lamp through in vitro disinfection tests and disinfection tests on artificially contaminated surfaces.</p><p><strong>Methods and results: </strong>The UV-C LED-based lamp efficacy was assessed at different initial microbial contamination titers and several UV doses, and both American Type Culture Collection (ATCC), foodborne, and clinical strains were considered. The UV-C LED lamp demonstrated high efficacy (log10 reduction >1 log) against the standard bacteria strains tested using a UV dose of 21.77 mJ cm-2. The greatest efficacy was achieved against E. coli (k = 0.0232) followed then by Bacillus subtilis (k = 0.0225) against which a titer of <1 CFU ml-1 was achieved with a UV dose of 15.55 and 21.77 mJ cm-2, respectively. Cladosporium spp. (k = 0.001) showed higher resistance against UV treatment, where a 50.00 ± 14.14 inactivation rate % (%IR) was achieved by applying the highest UV dose (31.1 mJ cm-2). Compared with B. subtilis, isolated L. monocytogenes 490 showed similar susceptibility (k = 0.0236), unlike isolated Listeria monocytogenes 1484 (k = 0.0146), isolated Salmonella Infantis 43 072/20 (k = 0.0126), and isolated S. Infantis 29 673/20 (k = 0.0124), which showed greater UV resistance. Considering the results obtained on the surfaces (stainless steel, polypropylene, and glass), the type of surface material influenced the susceptibility of isolated bacterial strains. However, the presence of organic matter (5% fetal bovine serum) on the treatment surface did not significantly affect device decontamination efficiency by applying a UV dose of 15.55, 21.77, and 31.1 mJ cm-2. On both stainless steel and glass, a titer <1 CFU 169 cm-2 was achieved against all the isolated bacterial strains applying a UV dose of 31.1 mJ cm-2, except with L. monocytogenes 1484. Lastly, a titer <1 CFU 169 cm-2 was never achieved on polypropylene contaminated with L. monocytogenes 1484.</p><p><strong>Conclusion: </strong>The use of a UV-C LED handheld lamp (peak wavelength 265 nm) could be an efficient disinfection method to be applied in domestic or small-scale food-processing environments, to reduce the cross contamination of food.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143663628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The overlooked interaction of emerging contaminants and microbial communities: a threat to ecosystems and public health.","authors":"Inês B Gomes","doi":"10.1093/jambio/lxaf064","DOIUrl":"10.1093/jambio/lxaf064","url":null,"abstract":"<p><strong>Context and aims: </strong>Emerging contaminants (ECs) and microbial communities should not be viewed in isolation, but through the One Health perspective. Both ECs and microorganisms lie at the core of this interconnected framework, as they directly influence the health of humans, animals, and the environment.The interactions between ECs and microbial communities can have profound implications for public health, affecting all three domains. However, these ECs-microorganism interactions remain underexplored, potentially leaving significant public health and ecological risks unrecognized. Therefore, this article seeks to alert the scientific community to the overlooked interactions between ECs and microbial communities, emphasizing the pivotal role these interactions may play in the management of 'One Health.'</p><p><strong>Results: </strong>The most extensively studied interaction between ECs and microbial communities is biodegradation. However, other more complex and concerning interactions demand attention, such as the impact of ECs on microbial ecology (disruptions in ecosystem balance affecting nutrient and energy cycles) and the rise and spread of antimicrobial resistance (a growing global health crisis). Although these ECs-microbial interactions had not been extensively studied, there are scientific evidence that ECs impact on microbial communities may be concerning for public health and ecosystem balance.</p><p><strong>Conclusions: </strong>So, this perspective summarizes the impact of ECs through a One Health lens and underscores the urgent need to understand their influence on microbial communities, while highlighting the key challenges researchers must overcome. Tackling these challenges is vital to mitigate potential long-term consequences for both ecosystems and public health.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Availability and cost of antifungal therapy in Vietnam: A 5-year retrospective study.","authors":"Yee Chin Kwang, Ha Thuy Nguyen, Jan-Willem Alffenaar, Justin Beardsley, Vu Quoc Dat","doi":"10.1093/mmy/myaf028","DOIUrl":"https://doi.org/10.1093/mmy/myaf028","url":null,"abstract":"<p><p>Access to antifungal agents for the treatment of invasive fungal infections (IFIs) varies significantly between countries. Limited access or high cost may contribute to the burden of IFIs. We aimed to investigate the availability and cost of antifungal treatment for IFIs in Vietnam. Procurement data from 2018 to 2022 was collected from the Drug Administration of Vietnam website. We calculated the cost per defined daily dose (DDD) and identified the manufacturing countries. We explored the pharmacotherapy cost of the four major IFIs if first-line agents were used in accordance with the Vietnam 2021 antifungal prescribing guideline. We also estimated the treatment expenditure in 2020 based on the estimated disease burden previously published and suggested cost-saving measures. At least 57.6 million USD was spent on 15.5 million DDD of antifungals in five years. Seven systemic antifungal agents were available in Vietnam. Caspofungin and micafungin were the least used but most expensive, whereas fluconazole and itraconazole were the most consumed but cheapest antifungals. Vietnam manufactured 70% of azole antifungals and relied on imports for the remaining antifungals consumed. We estimated the first-line pharmacological treatment for the estimated cases of four IFIs in 2020 to cost at least 209.1 million USD, which exceeded the actual spend in 2020. We discovered that antifungal agents for IFIs impose a substantial economic burden on Vietnam's healthcare system. We highlight the need for cost-effectiveness studies of expensive first-line medications. Efforts to mitigate this economic burden should include antifungal stewardship, prevention of IFIs, and sourcing from cost-effective manufacturers.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143763981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lisianthus Fusaria wilt: inter- and intra-specific variation in virulence of pathogens and biocontrol of the disease based on vietnamese Streptomyces goshikiensis STR61.","authors":"Dung Le, Thuy Thi Le, Tuong Manh Ho, Duong Thai Binh Pham","doi":"10.1093/jambio/lxaf079","DOIUrl":"10.1093/jambio/lxaf079","url":null,"abstract":"<p><strong>Aims: </strong>The study aimed to compare the pathogenicity of Vietnamese Fusaria species causing lisianthus Fusaria wilt (LFW) and to isolate, identify, and evaluate the potential of native Streptomyces for disease control.</p><p><strong>Methods and results: </strong>Pathogenicity assays on 11 Vietnamese isolates of Fusarium vanleeuwenii, Neocosmospora solani, and F. annulatum revealed significant inter- and intra-specific variations in virulence. Fusarium vanleeuwenii was the most aggressive, followed by N. solani, while F. annulatum was the least pathogenic. Screening of rhizosphere soil led to the identification of S. goshikiensis STR61, which exhibited strong antifungal activity against the growth of F. oxysporum, F. fujikuroi, and F. incarnatum-equiseti species complexes by over 69%, but showed lower efficacy (43.4%) against F. solani species complex. In pot assay against F. vanleeuwenii-inducing LFW, STR61 significantly reduced disease incidence (76.3%) and severity (80.6%), while promoting plant growth.</p><p><strong>Conclusions: </strong>The outcome of LFW was driven by species and isolates, with F. vanleeuwenii being most aggressive. Streptomyces goshikiensis STR61 can be applied as a biocontrol agent against Fusaria diseases.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143742848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pressure up to 60 bar has no major effect on the overall hydrogen consumption of the sulfate reducer Oleidesulfovibrio alaskensis.","authors":"Nicole Dopffel, Behruz Shaker Shiran, Kyle Mayers, Biwen Annie An-Stepec, Abduljelil Kedir, Ben Heydolph, Hadi Hajibeygi, Ketil Djurhuus","doi":"10.1093/jambio/lxaf077","DOIUrl":"https://doi.org/10.1093/jambio/lxaf077","url":null,"abstract":"<p><strong>Aims: </strong>Subsurface environments found in geological aquifers or reservoirs are not sterile but harbor diverse microbial communities for which hydrogen (H2) is a ubiquitous electron donor, especially for sulfate-reducing bacteria (SRB). Most studies investigating SRBs have been performed consumption experiments at near-atmospheric pressure. However, pressures are significantly higher in subsurface formations. It remains a crucial question whether high H2 partial pressure influences microbial consumption. Therefore, we tested a relevant SRB under increased H2-pressure to investigate changes in H2-consumption behavior.</p><p><strong>Methods and results: </strong>We cultured the H2-consuming SRB Oleidesulfovibrio alaskensis G20 under 1, 30, and 60 bar of H2 overpressure and quantified consumption over time. Data were compared to sterile incubations. After 16 days, the total amount of consumed H2, sulfate and acetate was similar for all pressure conditions and pH ended over 9, which is beyond the described pH limit. While the maximum H2 consumption rate was found higher at atmospheric pressures (0.20 mmol per day) compared to 30 and 60 bar (0.13 and 0.11 mmol per day), the maximum rate per surface area was comparable (0.02, 0.03, 0.02 mmol per day per cm2). The total rate of H2 consumption per cm2 was higher with increasing pressure, which is probably related to the increased solubility of H2 in the brine phase due to pressure.</p><p><strong>Conclusions: </strong>The data shows that pressures up to 60 bar have no significant effect on the overall activity of O. alaskensis. The governing factor for the H2 consumption rate is contact area between brine and gas phase and the concentration of dissolved H2.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143752744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Examining the potential prebiotic effect of almonds.","authors":"Maha Tahiri, Jack A Gilbert","doi":"10.1093/jambio/lxaf078","DOIUrl":"https://doi.org/10.1093/jambio/lxaf078","url":null,"abstract":"<p><p>Almonds, a nutrient-dense food rich in dietary fiber, polyphenols, and unsaturated fatty acids, exhibit significant potential as a functional food with prebiotic effects. Prebiotics selectively stimulate the growth and activity of beneficial gut microbiota, leading to improved gut and systemic health. This review synthesizes evidence from in vitro studies, clinical trials, and systematic reviews to elucidate the prebiotic effects of almond consumption. Almonds enhance gut microbiota diversity and composition, particularly increasing beneficial bacteria such as Bifidobacterium and Roseburia, while promoting the production of short-chain fatty acids (SCFAs), such as butyrate, which are critical for gut barrier integrity and inflammation modulation. The presence of polyphenols, such as proanthocyanidins, contributes to their antioxidative and antimicrobial properties, further supporting microbiome health. Despite variability in study outcomes, likely due to differences in population health status, study design, and almond preparation methods, the cumulative findings underscore almonds' role as a potential prebiotic food with the potential to improve cardiovascular health. Continued research focusing on individualized responses and standardized methodologies is essential to fully harness the health benefits of almond consumption.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143742847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocontrol Potential of Borrelidin Metabolites Derived from Streptomyces rochei A144 as a Fungicide.","authors":"Ning Wang, Wei Huang, Qiong Jia, Bo Song, Suling Wang, Longyuan Wu, Meng Sun, Yanzhi Wang, Lijuan Zhang, Wei Wang","doi":"10.1093/jambio/lxaf073","DOIUrl":"https://doi.org/10.1093/jambio/lxaf073","url":null,"abstract":"<p><strong>Aims: </strong>This study aimed to isolate and identify antifungal metabolites and evaluate potential applications for biocontrol.</p><p><strong>Methods and results: </strong>Using a bioactivity-guided fractionation approach, we obtained the macrolide metabolite borrelidin from Streptomyces rochei A144, which exhibited significant inhibitory effects on V. mali mycelial growth (EC50 = 22.23 μg mL-1). Scanning and transmission electron microscopy analyses revealed that borrelidin caused damage to V. mali hyphae, such as breakage, increased swelling and branching at the hyphal tips, irregular cell wall thickness, plasmolysis, and degeneration of cellular organelles. After borrelidin treatment, the lesion length on detached twigs and lesion area on leaves were reduced by 49.38% and 89.16%, respectively. The mycelial growth rate method was used to evaluate the antifungal activity of borrelidin against various plant pathogenic fungi. The study findings indicate that borrelidin possesses broad-spectrum antifungal activity, with inhibition rates in the range of 21.32%-100%.</p><p><strong>Conclusions: </strong>The macrolide metabolite borrelidin, derived from S. rochei A144, exhibited significant antifungal activity against V. mali and broad-spectrum inhibition of phytopathogenic fungi.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143692055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid and Efficient CRISPR-Based Detection of Dengue Virus in a Single-Tube.","authors":"Jiaye Zhong, Juezhuo Li, Shiyu Chen, Yue Xu, Xiaolei Mao, Minghui Xu, Shuyin Luo, Yi Yang, Jiawei Zhou, Jinghua Yuan, Lan Su, Gang Wang, Xinling Zhang, Xiaoping Li","doi":"10.1093/jambio/lxaf070","DOIUrl":"https://doi.org/10.1093/jambio/lxaf070","url":null,"abstract":"<p><strong>Background: </strong>Dengue Virus (DENV) is prevalent in tropical and subtropical regions. With the projected climate change, traditional detection methods face limitations, and there is an urgent need for more accurate and efficient diagnostic techniques.</p><p><strong>Objective: </strong>The aim is to integrate Recombinase-aided Amplification (recombinase-aided Amplification, RAA) and Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) to establish a special CRISPR-Cas system for rapid, convenient, high sensitivity, and high specificity typing detection of DENV.</p><p><strong>Methods: </strong>CRISPR RNA (crRNA) and RAA primers were designed based on the whole genome of four DENV serotypes. A single-tube assay combining RAA with CRISPR-Cas13a technology was developed after optimizing reagent concentrations.</p><p><strong>Results: </strong>The limit of detection (LoD) of DENV types 1-4 was 10³ copies·mL -1. No cross-reaction was observed between any of the DENV serotypes and the other three flaviviruses (Zika, West Nile, and Murray Valley encephalitis). The average sensitivity of One-step method was 95.8%, and the average specificity was 96.6%. Fluorescent signal intensities demonstrated a clear dose-dependent response, with the signal increasing as the sample concentration rose. This system can effectively distinguish non-target substances. Among them, One-step method has advantages in timeliness, ease of operation, and contamination control because it runs efficiently inside a tube and does not require the lid to be removed, but its sensitivity is relatively low. The Two-steps method performs well in sensitivity.</p><p><strong>Conclusion: </strong>In this study, we developed a novel method for rapid typing and detection of DENV using RAA and CRISPR-Cas13a in a single-tube homogeneous system.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143648736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial activity of peptoids against Metallo-β-lactamase-producing Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and other WHO priority pathogens, including Candida auris.","authors":"Shyam Kumar Mishra, Muhammad Yasir, Rajesh Kuppusamy, Edgar H H Wong, Alex Hui, Kristian Sørensen, Jennifer S Lin, Håvard Jenssen, Annelise E Barron, Mark Willcox","doi":"10.1093/jambio/lxaf031","DOIUrl":"10.1093/jambio/lxaf031","url":null,"abstract":"<p><strong>Aims: </strong>The World Health Organization has identified ESKAPE bacteria and Candida auris as priority pathogens, emphasizing an urgent need for novel antimicrobials to combat them. This study aimed to explore the therapeutic potential of antimicrobial peptidomimetics, specifically peptoids with sequence-specific N-substituted glycines, against ESKAPEE pathogens, including metallo-β-lactamase (MBL) producers, as well as C. auris strains.</p><p><strong>Methods and results: </strong>This study evaluated activity of the peptoids against the multidrug-resistant priority pathogens. The peptoid TM8 (with an N-decyl alkyl chain) demonstrated a geometric mean minimum inhibitory concentration (MIC) of 7.8 μg ml-1 against MBL-producing bacteria, and 5.5 μg ml-1 against C. auris. TM8 showed synergy with ciprofloxacin, enhancing its effectiveness 4-fold against NDM-1-producing Klebsiella pneumoniae. No antagonism was seen when TM8 was used with either conventional antibiotics or antifungals. Peptoids that had therapeutic indices below 3 were generally more hydrophobic, due to either alkyl chains or bromine. Scanning electron microscopy and live-dead staining assay on peptoid-treated C. auris confirmed morphological changes and killing activity, respectively. Furthermore, the peptoid could effectively inhibit biofilm formation by C. auris.</p><p><strong>Conclusion: </strong>Peptoids demonstrated antibacterial activity against ESKAPEE, particularly against MBL-producing Gram-negative bacteria. Additionally, they exhibited antifungal and anti-biofilm activities against C. auris strains.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11879280/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143398973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}