{"title":"兔源乳酸菌对益生元低聚糖的代谢：菌株特异性利用和应用的见解","authors":"Guizhen Gong, Angmu Duojie, Yurong He, Jianxia Yang, Xinrui Zhang, Yang Chen, Yuanting Zhu","doi":"10.1093/jambio/lxaf182","DOIUrl":null,"url":null,"abstract":"<p><strong>Aims: </strong>To investigate the in vitro utilization of functional oligosaccharides by lactic acid bacteria (LAB) isolated from rabbits, characterize their metabolic kinetics and genomic determinants, and evaluate their in vitro probiotic traits when grown on oligosaccharides.</p><p><strong>Methods and results: </strong>Ninety-two LAB strains from weaned rabbits were screened for utilization of isomaltose- (IMO), galactose- (GOS), chitosan- (COS), and mannose-oligosaccharides (MOS). Strain- and substrate-specific utilization patterns were observed: 63% utilized IMO, 68.5% GOS, 16.3% MOS, and none metabolized COS. Lacticaseibacillus paracasei YT170-selected for its broad substrate range-showed distinct growth kinetics and pH dynamics on IMO, GOS, and MOS. Genomic analysis revealed specialized carbohydrate-active enzymes (CAZymes) for each oligosaccharide. After 72 h fermentation by YT170, cell-free supernatants (CFS) from three oligosaccharides exhibited enhanced antioxidant activities (DPPH scavenging > 88%) and substrate-dependent antimicrobial effects: MOS fermentation most effectively inhibited Escherichia coli (ZOI: 21.30 ± 0.66 mm vs. LGG 19.33 ± 0.29 mm), while GOS fermentation showed maximal activity against Staphylococcus aureus (ZOI: 25.17 ± 0.95 mm).</p><p><strong>Conclusion: </strong>Oligosaccharide utilization by LAB from rabbits is strain- and substrate-specific, and L. paracasei YT170 demonstrates metabolic versatility, enabling synbiotic design with IMO, GOS, or MOS to modulate gut microbiota in weaned rabbits, but further in vivo studies are required.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2000,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Metabolism of Prebiotic Oligosaccharides by Lactic Acid Bacteria Isolated From Rabbits: Insights into Strain-Specific Utilization and Applications.\",\"authors\":\"Guizhen Gong, Angmu Duojie, Yurong He, Jianxia Yang, Xinrui Zhang, Yang Chen, Yuanting Zhu\",\"doi\":\"10.1093/jambio/lxaf182\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Aims: </strong>To investigate the in vitro utilization of functional oligosaccharides by lactic acid bacteria (LAB) isolated from rabbits, characterize their metabolic kinetics and genomic determinants, and evaluate their in vitro probiotic traits when grown on oligosaccharides.</p><p><strong>Methods and results: </strong>Ninety-two LAB strains from weaned rabbits were screened for utilization of isomaltose- (IMO), galactose- (GOS), chitosan- (COS), and mannose-oligosaccharides (MOS). Strain- and substrate-specific utilization patterns were observed: 63% utilized IMO, 68.5% GOS, 16.3% MOS, and none metabolized COS. Lacticaseibacillus paracasei YT170-selected for its broad substrate range-showed distinct growth kinetics and pH dynamics on IMO, GOS, and MOS. Genomic analysis revealed specialized carbohydrate-active enzymes (CAZymes) for each oligosaccharide. After 72 h fermentation by YT170, cell-free supernatants (CFS) from three oligosaccharides exhibited enhanced antioxidant activities (DPPH scavenging > 88%) and substrate-dependent antimicrobial effects: MOS fermentation most effectively inhibited Escherichia coli (ZOI: 21.30 ± 0.66 mm vs. LGG 19.33 ± 0.29 mm), while GOS fermentation showed maximal activity against Staphylococcus aureus (ZOI: 25.17 ± 0.95 mm).</p><p><strong>Conclusion: </strong>Oligosaccharide utilization by LAB from rabbits is strain- and substrate-specific, and L. paracasei YT170 demonstrates metabolic versatility, enabling synbiotic design with IMO, GOS, or MOS to modulate gut microbiota in weaned rabbits, but further in vivo studies are required.</p>\",\"PeriodicalId\":15036,\"journal\":{\"name\":\"Journal of Applied Microbiology\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":3.2000,\"publicationDate\":\"2025-07-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Applied Microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1093/jambio/lxaf182\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Applied Microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1093/jambio/lxaf182","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
目的：研究兔源乳酸菌（LAB）对功能性低聚糖的体外利用，表征其代谢动力学和基因组决定因素，并评价其在低聚糖上生长的体外益生菌特性。方法与结果：筛选92株断奶家兔乳酸菌对异麦芽糖- (IMO)、半乳糖- (GOS)、壳聚糖- (COS)和甘露寡糖（MOS）的利用情况。菌株和底物特异性利用模式：63%利用IMO， 68.5%利用GOS， 16.3%利用MOS，没有代谢COS。副干酪乳杆菌yt170因其广泛的底物范围而被选中，在IMO、GOS和MOS上表现出不同的生长动力学和pH动力学。基因组分析显示每种低聚糖都有专门的碳水化合物活性酶（CAZymes）。经YT170发酵72 h后，三种低聚糖的无细胞上清液（CFS）均表现出较强的抗氧化活性（DPPH清除率bbbb88%）和底物依赖性抗菌作用：MOS发酵对大肠杆菌（ZOI: 21.30±0.66 mm vs. LGG 19.33±0.29 mm）最有效，GOS发酵对金黄色葡萄球菌（ZOI: 25.17±0.95 mm）抑制作用最强。结论：乳酸菌对家兔低聚糖的利用具有菌株和底物特异性，副干酪乳杆菌YT170具有代谢多功能性，可以通过与IMO、GOS或MOS的合成设计来调节断奶家兔的肠道微生物群，但还需要进一步的体内研究。
Metabolism of Prebiotic Oligosaccharides by Lactic Acid Bacteria Isolated From Rabbits: Insights into Strain-Specific Utilization and Applications.
Aims: To investigate the in vitro utilization of functional oligosaccharides by lactic acid bacteria (LAB) isolated from rabbits, characterize their metabolic kinetics and genomic determinants, and evaluate their in vitro probiotic traits when grown on oligosaccharides.
Methods and results: Ninety-two LAB strains from weaned rabbits were screened for utilization of isomaltose- (IMO), galactose- (GOS), chitosan- (COS), and mannose-oligosaccharides (MOS). Strain- and substrate-specific utilization patterns were observed: 63% utilized IMO, 68.5% GOS, 16.3% MOS, and none metabolized COS. Lacticaseibacillus paracasei YT170-selected for its broad substrate range-showed distinct growth kinetics and pH dynamics on IMO, GOS, and MOS. Genomic analysis revealed specialized carbohydrate-active enzymes (CAZymes) for each oligosaccharide. After 72 h fermentation by YT170, cell-free supernatants (CFS) from three oligosaccharides exhibited enhanced antioxidant activities (DPPH scavenging > 88%) and substrate-dependent antimicrobial effects: MOS fermentation most effectively inhibited Escherichia coli (ZOI: 21.30 ± 0.66 mm vs. LGG 19.33 ± 0.29 mm), while GOS fermentation showed maximal activity against Staphylococcus aureus (ZOI: 25.17 ± 0.95 mm).
Conclusion: Oligosaccharide utilization by LAB from rabbits is strain- and substrate-specific, and L. paracasei YT170 demonstrates metabolic versatility, enabling synbiotic design with IMO, GOS, or MOS to modulate gut microbiota in weaned rabbits, but further in vivo studies are required.
期刊介绍：
Journal of & Letters in Applied Microbiology are two of the flagship research journals of the Society for Applied Microbiology (SfAM). For more than 75 years they have been publishing top quality research and reviews in the broad field of applied microbiology. The journals are provided to all SfAM members as well as having a global online readership totalling more than 500,000 downloads per year in more than 200 countries. Submitting authors can expect fast decision and publication times, averaging 33 days to first decision and 34 days from acceptance to online publication. There are no page charges.
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