Lactic acid bacteria strains isolated from rehydrated corn grain silage enhance the chemical and microbiological characteristics and aerobic stability of these silages.

IF 3.2 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of Applied Microbiology Pub Date : 2025-07-22 DOI:10.1093/jambio/lxaf185

Yasmim A Silva, Marcus V S B Cardoso, Beatriz F Carvalho, Rosane F Schwan, Carla L S Ávila

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引用次数: 0

Abstract

Aims: This study aimed to isolate, characterize, and select lactic acid bacteria (LAB) strains from rehydrated corn grain silages (RCGSs) and evaluated the effects of these strains as inoculants for improving silage chemical composition, microbiological quality, and aerobic stability.

Methods and results: One hundred and forty LAB were isolated from RCGSs, characterized, and subjected to growth and pH reduction tests in corn grain extract. Thirty-nine strains were evaluated for metabolite production, starch degradation, and inhibitory activity against Escherichia coli and Bacillus cereus. Selected strains (21) included Pediococcus acidilactici (PA), P. pentosaceus (PP), Lactiplantibacillus plantarum (LP); and Limosilactobacillus fermentum (LF). After 60 days of storage, strains 180 (PA) and 190 (PP) showed the greatest pH reduction (3.69) and lactic acid production (4.72%) but lower aerobic stability (AS) (27 h). The AS was highest in silages inoculated with strains 185 (PP) and 126 (PA) (92.3 h). Inoculation with strains 221 (PP) and 116 (LF) (1.95%) had the lowest dry matter (DM) losses (1.95%), while strains 77, 79 (PA); 190, 110, and 52 (PP) minimized starch losses (8.22%).

Conclusion: RCGS epiphytic strains can improve silage fermentation profiles, each strain contributing specific benefits based on its metabolism.

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从复水化玉米青贮中分离得到的乳酸菌菌株提高了青贮的化学、微生物特性和有氧稳定性。

目的：本研究旨在分离、鉴定和筛选水合玉米青贮（RCGSs）中乳酸菌（LAB）菌株，并评价这些菌株作为接种剂对青贮化学成分、微生物品质和有氧稳定性的影响。方法与结果：从rgss中分离得到140个LAB，对其进行了表征，并在玉米籽粒提取物中进行了生长和pH还原试验。评估了39株菌株的代谢物产量、淀粉降解以及对大肠杆菌和蜡样芽孢杆菌的抑制活性。所选菌株21株，包括嗜酸Pediococcus （PA）、P. pentosaceus （PP）、plantibacillus plantarum (LP)；和发酵乳酸杆菌（LF）。贮藏60 d后，菌株180 （PA）和190 （PP）的pH值降低幅度最大（3.69），乳酸产量最高（4.72%），但好氧稳定性（AS）较低（27 h）。以菌株185 （PP）和126 （PA）接种的青贮饲料AS最高（92.3 h）。接种菌株221 （PP）和116 (LF)（1.95%）的干物质（DM）损失最低（1.95%），接种菌株77、79 （PA）的DM损失最低；190、110和52 （PP）使淀粉损失最小（8.22%）。结论：RCGS附生菌株可改善青贮发酵特性，各菌株根据其代谢有不同的效益。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Applied Microbiology 生物-生物工程与应用微生物

CiteScore

7.30

自引率

2.50%

发文量

427

审稿时长

2.7 months

期刊介绍： Journal of & Letters in Applied Microbiology are two of the flagship research journals of the Society for Applied Microbiology (SfAM). For more than 75 years they have been publishing top quality research and reviews in the broad field of applied microbiology. The journals are provided to all SfAM members as well as having a global online readership totalling more than 500,000 downloads per year in more than 200 countries. Submitting authors can expect fast decision and publication times, averaging 33 days to first decision and 34 days from acceptance to online publication. There are no page charges.