Gut dysbiosis allows foodborne salmonella colonization in edible crickets: a probiotic strategy for enhanced food safety.

IF 3.2 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of Applied Microbiology Pub Date : 2025-10-06 DOI:10.1093/jambio/lxaf217

Shuma Tsuji, Osamu Matsushita, Jumpei Uchiyama, Kenji Yokota, Tetsuya Bando, Hideyo Ohuchi, Kazuyoshi Gotoh

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引用次数: 0

Abstract

Aims: Edible insects, including crickets, represent a promising protein source, yet concerns over foodborne pathogens limit consumer acceptance. This study investigated whether gut microbiota modulates colonization by Salmonella enterica subsp. enterica serovar Enteritidis (SE) in the two-spotted cricket (Gryllus bimaculatus).

Methods and results: Under standard conditions, SE was undetectable in crickets despite prolonged exposure; however, antibiotic-induced dysbiosis enabled stable SE colonization. Long-read 16S rRNA sequencing revealed significant microbiota shifts, notably a reduction in Lactococcus garvieae. In vitro assays showed strong inhibitory effects of L. garvieae against SE, and supplementation of dysbiotic crickets with L. garvieae reduced SE colonization by ∼1000-fold.

Conclusions: The native cricket gut microbiota, especially L. garvieae, plays a protective role against SE colonization. Enhancing beneficial gut bacteria could mitigate pathogen risks and promote edible insects as a sustainable protein.

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肠道失调允许食源性沙门氏菌在食用蟋蟀中定植：提高食品安全的益生菌策略。

目的：食用昆虫，包括蟋蟀，是一种很有前途的蛋白质来源，然而对食源性病原体的担忧限制了消费者的接受度。本研究探讨肠道菌群是否调节肠道沙门氏菌亚种的定植。双斑蟋蟀（Gryllus bimaculatus）的血清型肠炎（SE）。方法与结果：在标准条件下，尽管长时间暴露于蟋蟀体内，但检测不到SE；然而，抗生素诱导的生态失调使SE稳定定植。长读16S rRNA测序显示了显著的微生物群变化，特别是乳球菌的减少。体外实验表明，L. garvieae对SE有很强的抑制作用，在益生蟋蟀中添加L. garvieae可使SE的定植减少约1000倍。结论：本地蟋蟀肠道微生物群，尤其是L. garvieae对SE定殖具有保护作用。增强有益的肠道细菌可以降低病原体的风险，并促进食用昆虫作为可持续蛋白质。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Applied Microbiology 生物-生物工程与应用微生物

CiteScore

7.30

自引率

2.50%

发文量

427

审稿时长

2.7 months

期刊介绍： Journal of & Letters in Applied Microbiology are two of the flagship research journals of the Society for Applied Microbiology (SfAM). For more than 75 years they have been publishing top quality research and reviews in the broad field of applied microbiology. The journals are provided to all SfAM members as well as having a global online readership totalling more than 500,000 downloads per year in more than 200 countries. Submitting authors can expect fast decision and publication times, averaging 33 days to first decision and 34 days from acceptance to online publication. There are no page charges.