Leandro Fonseca de Souza, Fernanda Mancini Nakamura, Marie Kroeger, Dasiel Obregon Alvarez, Moacir Tuzzin de Moraes, Mariana Gomes Vicente, Marcelo Zacharias Moreira, Vivian Helena Pellizari, Siu Mui Tsai, Klaus Nüsslein
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引用次数: 0
Abstract
Aims: In the Amazon region, pastures are the main land use subsequent to deforestation and this change can result in soil acidification and degradation. Liming is a management practice to increase soil pH, important to recover degraded lands and increase soil fertility, but its impacts on soil methane cycling in tropical soils is unknown. Here we investigate the role of soil pH on methane uptake under high concentrations of the gas, manipulating pasture and forest soils pH by liming and evaluating the active methane cycling microbial community.
Methods and results: Top layer of forest and pasture soils were subjected to liming treatment and incubated with ∼10,000 ppm of 13CH4. Soil DNA was evaluated with Stable Isotopic Probing (SIP-DNA), methanotrophic abundance was quantified (pmoA gene), and high throughput sequencing of 16S rRNA was performed. Liming increased the methane uptake in both forest (∼10%) and pasture (∼25%) soils. Methanotrophs Methylocaldum sp. (type I) and potential methanotrophs in Beijerinckiaceae (type II) were identified to actively incorporate carbon from methane in limed pasture soils. In limed forest soils, Nitrososphaeraceae were identified as 13C-enriched taxa, indicating that ammonia oxidizers can oxidize methane in these soils.
Conclusions: Liming Amazonian pasture soils not only contributes to the fertility and recovery of degraded areas, but has the potential to improve the oxidation of methane at high concentrations of this gas.
期刊介绍:
Journal of & Letters in Applied Microbiology are two of the flagship research journals of the Society for Applied Microbiology (SfAM). For more than 75 years they have been publishing top quality research and reviews in the broad field of applied microbiology. The journals are provided to all SfAM members as well as having a global online readership totalling more than 500,000 downloads per year in more than 200 countries. Submitting authors can expect fast decision and publication times, averaging 33 days to first decision and 34 days from acceptance to online publication. There are no page charges.