Journal of Applied Microbiology最新文献_第6页

Propolis-derived components act against Klebsiella pneumoniae by inhibiting growth and disrupting biofilms. 蜂胶衍生成分通过抑制生长和破坏生物膜来对抗肺炎克雷伯菌。

IF 3.2 3区生物学

Journal of Applied Microbiology Pub Date : 2025-09-01 DOI: 10.1093/jambio/lxaf222

Ting Li, Jie Yuan, Shuangshuang Gao, Yujing Tang, Hongzhuan Xuan

{"title":"Propolis-derived components act against Klebsiella pneumoniae by inhibiting growth and disrupting biofilms.","authors":"Ting Li, Jie Yuan, Shuangshuang Gao, Yujing Tang, Hongzhuan Xuan","doi":"10.1093/jambio/lxaf222","DOIUrl":"10.1093/jambio/lxaf222","url":null,"abstract":"Aims: Klebsiella pneumoniae (K. pneumoniae), a multidrug-resistant opportunistic pathogen implicated in pneumonia and nosocomial infections, employs biofilm formation to evade antimicrobial therapies.Methods: This study investigates how propolis and its key bioactive constituents-naringenin, taxifolin, syringic acid, and gallic acid-disrupt biofilm development and stability in K. pneumoniae. Biofilm inhibition was evaluated using crystal violet staining, adhesion assays, and scanning electron microscopy (SEM), while membrane integrity was assessed via LDH release and Live/Dead fluorescence. Molecular docking predicted interactions between compounds and biofilm-associated proteins, complemented by qRT-PCR analysis of virulence genes (fimH, ompA, mrkA, mrkD).Results: All compounds demonstrated dose-dependent antibiofilm effects, with propolis showing superior efficacy (79.1% biofilm inhibition at 2 MIC). Propolis and its components reduced bacterial adhesion by 50%-81%, disrupted extracellular matrix architecture, and increased non-viable cell ratios (Live/Dead: 30.55% dead cells in taxifolin-treated biofilms). Taxifolin exhibited the strongest binding affinity (-7.8 kcal·mol-1) for the OmpA protein. Gene expression analysis showed that propolis, naringenin, taxifolin, and gallic acid substantially downregulated biofilm-associated genes, including fimH, ompA, mrkA, and mrkD.Conclusions: Propolis disrupts K. pneumoniae biofilm formation via multi-target regulation, with taxifolin as a potential key bioactive monomer. The results demonstrate the potential of propolis and its main active components to inhibit K. pneumoniae.","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144955313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The severity of subacute ruminal acidosis of primiparous transition cows affects the fecal metabolome, without affecting the fecal microbiome of the cows or their calves. 初产过渡期奶牛亚急性瘤胃酸中毒的严重程度影响粪便代谢组，但不影响奶牛或犊牛的粪便微生物组。

IF 3.2 3区生物学

Journal of Applied Microbiology Pub Date : 2025-09-01 DOI: 10.1093/jambio/lxaf213

Ezequias Castillo-Lopez, Thomas Hartinger, Sara Ricci, Kangkang Xu, Heidi Schwartz-Zimmerman, Franz Berthiller, Nicole Reisinger, Qendrim Zebeli

{"title":"The severity of subacute ruminal acidosis of primiparous transition cows affects the fecal metabolome, without affecting the fecal microbiome of the cows or their calves.","authors":"Ezequias Castillo-Lopez, Thomas Hartinger, Sara Ricci, Kangkang Xu, Heidi Schwartz-Zimmerman, Franz Berthiller, Nicole Reisinger, Qendrim Zebeli","doi":"10.1093/jambio/lxaf213","DOIUrl":"10.1093/jambio/lxaf213","url":null,"abstract":"Aims: To evaluate the effects of the severity of subacute ruminal acidosis (SARA) on the fecal microbiome and metabolome of primiparous transition cows, and the fecal microbiome and blood composition of the offspring; to investigate the effect of transition from gestation to lactation on the fecal microbiome of cows.Methods and results: The study included 24 cows; three SARA severities were evaluated: low, moderate, and high. Analyses of the fecal microbiome and metabolome of cows were performed from week 3 before parturition until week 10 in lactation; the fecal microbiome of calves was evaluated at 1 week of age. Blood composition of calves was evaluated at days 1 and 7. Compared to low, greater SARA severity did not affect the fecal microbial diversity or abundance in cows, but increased concentrations of fecal amino acids and their derivatives in high SARA, resulting in enrichment of metabolic pathways for amino acid metabolism. No negative effects of SARA severity were found on the offspring's fecal microbiome or blood composition. Fecal amylolytic bacteria increased in cows postcalving at the expense of fibrolytic and methanogenic taxa.Conclusion: SARA severity influenced the fecal metabolome of transition cows, but no negative impacts were found on the fecal microbiome of cows or on the microbiome and blood composition of the offspring.","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144955164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic antimicrobial and antibiofilm effects of plant-active ingredients and antibiotics on multidrug-resistant Acinetobacter baumannii. 植物活性成分和抗生素对多重耐药鲍曼不动杆菌的协同抑菌和抗菌膜作用。

IF 3.2 3区生物学

Journal of Applied Microbiology Pub Date : 2025-09-01 DOI: 10.1093/jambio/lxaf211

Rukiye Aslan, Ahmet Alim

{"title":"Synergistic antimicrobial and antibiofilm effects of plant-active ingredients and antibiotics on multidrug-resistant Acinetobacter baumannii.","authors":"Rukiye Aslan, Ahmet Alim","doi":"10.1093/jambio/lxaf211","DOIUrl":"https://doi.org/10.1093/jambio/lxaf211","url":null,"abstract":"Aims: The increasing antimicrobial resistance, particularly in Acinetobacter baumannii, complicates the treatment of infections, leading to higher morbidity, mortality, and economic costs. Herein, we aimed to determine the in vitro antimicrobial, synergistic, and antibiofilm activities of colistin (COL), meropenem, and ciprofloxacin antibiotics, and curcumin, punicalagin, geraniol (GER), and linalool (LIN) plant-active ingredients alone and in combination against 31 multidrug-resistant (MDR) A. baumannii clinical isolates.Methods and results: The combinations were tested to identify alternative approaches to conventional antibiotic therapy by reducing the Minimum Inhibitory Concentration (MIC) values of antibiotics when used with plant-active ingredients. Synergistic interactions were evaluated by checkerboard assay and interpreted via the Fractional Inhibitory Concentration Index (FICI). Antibiofilm activity was assessed using crystal violet microtiter plate method. Notably, COL with GER (83.87%) and with LIN (77.42%) exhibited strong synergistic interactions, with FICI values between 0.12 and 0.5. Where synergism was observed, antibiotic MICs were reduced by 2- to 128-fold, indicating substantial enhancement in bacterial efficacy. Synergistic and additive interactions were more prevalent than indifference, and no antagonism was detected. Biofilm formation inhibition assays further demonstrated that these combinations significantly suppressed biofilm production in A. baumannii isolates. Biofilm eradication rates were consistently highest at 2 × MIC concentrations for all tested antibiotics and plant-active ingredients. Cytotoxicity tests on L929-fibroblast cell lines confirmed the safety at the tested concentrations.Conclusion: Combining conventional antibiotics with plant-active ingredients offers a novel approach to mitigating the impact of MDR A. baumannii.","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":"136 9","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145015423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Carbon assimilation and regulation by the phosphotransferase system in Actinobacillus succinogenes. 琥珀酸放线菌磷酸转移酶系统的碳同化与调控。

IF 3.2 3区生物学

Journal of Applied Microbiology Pub Date : 2025-09-01 DOI: 10.1093/jambio/lxaf232

Angélica Vallejo-Giraldo, Juan Carlos Fragoso-Jiménez, Luz María Martínez, Georgina Hernández-Chávez, Enrique Merino, Alfredo Martinez, Rosa Isela Corona-González, Esteban Marcellin, Claire Vieille, Guillermo Gosset

{"title":"Carbon assimilation and regulation by the phosphotransferase system in Actinobacillus succinogenes.","authors":"Angélica Vallejo-Giraldo, Juan Carlos Fragoso-Jiménez, Luz María Martínez, Georgina Hernández-Chávez, Enrique Merino, Alfredo Martinez, Rosa Isela Corona-González, Esteban Marcellin, Claire Vieille, Guillermo Gosset","doi":"10.1093/jambio/lxaf232","DOIUrl":"10.1093/jambio/lxaf232","url":null,"abstract":"Aims: Actinobacillus succinogenes is a succinate-producing, glucose-fermenting bacterium with phosphoenolpyruvate:sugar phosphotransferase system (PTS)-related genes identified in its genome. This study aimed to investigate the role of PTS in carbon source transport and regulation.Methods and results: A mutant strain was generated lacking genes encoding the non-sugar-specific PTS components EI (Asuc_0995), Hpr (Asuc_0994), and EIIAGlc (Asuc_0996). Characterization of this mutant revealed a partial role of PTS in glucose transport, while uptake of sucrose, cellobiose, mannose, mannitol, sorbitol, L-ascorbate, and fructose was abolished. PTS disruption also reduced consumption of the non-PTS carbon sources xylose, lactose, maltose, L-arabinose, ribose, and glycerol, indicating a regulatory role for the PTS in alternative carbon catabolism. Adding cAMP to cultures of the PTS-deficient mutant partially restored growth on some of the non-PTS carbon sources, suggesting a role of CRP-cAMP in regulating carbon source catabolism in A. succinogenes. Transcriptome and proteome analyses showed differential expression of carbohydrate transport and metabolism genes in the PTS-deficient strain compared to wild-type on glucose. Bioinformatic analyses predicted that several differentially expressed genes have a putative CRP-cAMP binding site in their upstream DNA regions.Conclusions: We showed that PTS components encoded by Asuc_0994-0996 are essential for transporting several sugars and partially involved in glucose uptake. Our findings also implicate the CRP-cAMP network in regulating non-PTS carbon catabolism.","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145091788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Carbapenem heteroresistance in Acinetobacter baumannii and Pseudomonas aeruginosa blood isolates from a Turkish tertiary hospital: a comprehensive study. 土耳其某三级医院鲍曼不动杆菌和铜绿假单胞菌血液分离株碳青霉烯类耐药的综合研究

IF 3.2 3区生物学

Journal of Applied Microbiology Pub Date : 2025-09-01 DOI: 10.1093/jambio/lxaf204

Ekin Kırbaş, Banu Sancak

{"title":"Carbapenem heteroresistance in Acinetobacter baumannii and Pseudomonas aeruginosa blood isolates from a Turkish tertiary hospital: a comprehensive study.","authors":"Ekin Kırbaş, Banu Sancak","doi":"10.1093/jambio/lxaf204","DOIUrl":"10.1093/jambio/lxaf204","url":null,"abstract":"Aims: To investigate the prevalence of carbapenem resistance and carbapenem heteroresistance (CAHR) in Acinetobacter baumannii and Pseudomonas aeruginosa blood isolates.Methods: The susceptibility of 408 bloodstream isolates (256 A. baumannii and 152 P. aeruginosa) to imipenem (IMI) and meropenem (MER) was determined using the broth microdilution method. CAHR was investigated by population analysis profiling (PAP) and diffusion-based tests, including disk diffusion and antibiotic gradient tests. Carbapenemase genes in carbapenem-resistant and carbapenem-heteroresistant isolates were identified by polymerase chain reaction.Results: Based on the Clinical Laboratory Standards Institute suceptibility breakpoints, the resistance rates for A. baumannii were 88.3% for IMI and 87.5% for MER, while P. aeruginosa showed resistance rates of 43.4% for IMI and 46.7% for MER. Among carbapenem-resistant A. baumannii strains, blaOXA-51 (100%) and blaOXA-23 (86.3%) were the most prevalent. Among carbapenem-resistant P. aeruginosa strains, blaOXA-10 (26.8%) was the most frequent. The PAP method revealed that the IMI- and MER-HR rates were 24.1% and 30%, respectively, in A. baumannii isolates, while in P. aeruginosa isolates, the rates were 57.9% and 13.8%, respectively. blaOXA-51 was detected in all carbapenem-resistant A. baumannii subpopulations, whereas blaOXA-23 was detected in 75% (n= 12) of isolates. No carbapenemase genes were identified in any of the carbapenem-resistant P. aeruginosa subpopulations.Conclusions: Upon comparison of the results obtained with diffusion-based tests and PAP, diffusion-based tests were found to be unreliable for detecting CAHR.","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144855238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The anti-biofilm activity of cannabinoids against methicillin-resistant Staphylococcus aureus. 大麻素对耐甲氧西林金黄色葡萄球菌的抗生物膜活性。

IF 3.2 3区生物学

Journal of Applied Microbiology Pub Date : 2025-09-01 DOI: 10.1093/jambio/lxaf214

Pancy Tsz Hei Kwong, Theerthankar Das, Jonathon Carl Arnold, Hak-Kim Chan, Philip Chi Lip Kwok

{"title":"The anti-biofilm activity of cannabinoids against methicillin-resistant Staphylococcus aureus.","authors":"Pancy Tsz Hei Kwong, Theerthankar Das, Jonathon Carl Arnold, Hak-Kim Chan, Philip Chi Lip Kwok","doi":"10.1093/jambio/lxaf214","DOIUrl":"10.1093/jambio/lxaf214","url":null,"abstract":"Aims: Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of hospital-acquired pneumonia with resistance against beta-lactam antibiotics. New, potent antibiotics against MRSA with other mechanisms of action are thus urgently needed. Recently, cannabinoids have been evaluated for antimicrobial activity in the ongoing search for new anti-infective agents, but their anti-biofilm effect has not been extensively studied. In this study, five main phytocannabinoids-cannabidiol (CBD), delta-9-tetrahydrocannabinol (THC), cannabinol (CBN), cannabigerol (CBG), and cannabichromene (CBC) were examined for their activity against MRSA biofilms.Methods and results: The anti-biofilm activity was assessed by crystal violet staining, resazurin metabolic assay, reactive oxygen species (ROS) assay, and propidium iodide membrane integrity test. The minimum inhibitory concentrations of all tested cannabinoids were between 1 and 2 µg/mL. CBN showed the most potent anti-MRSA biofilm activity, significantly reducing biofilm biomass and bacterial viability. It also induced the highest intracellular ROS levels. In contrast, CBD was the least effective among the tested cannabinoids in most of the anti-biofilm assays, yet it caused the greatest membrane damage to bacteria within the biofilm.Conclusions: This study showed that despite being chemically similar, the cannabinoids demonstrated different potency and potentially different mechanisms of action against MRSA. More research is needed to investigate how they act on this pathogen and its biofilm.","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144955091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel gelling agent similar to galactomannan from the sheath-forming bacterium Sphaerotilus hippei. 一种类似于半乳甘露聚糖的新型胶凝剂，来自于形成鞘的球形鹦鹉螺。

IF 3.2 3区生物学

Journal of Applied Microbiology Pub Date : 2025-09-01 DOI: 10.1093/jambio/lxaf220

Mishal Shafiq, Shiqian Chen, Naoki Matsunaga, Aoi Hagio, Ichiro Suzuki, Daisuke Koizumi, Rie Togawa, Yudai Yamaoki, Keiko Kondo, Masato Katahira, Minoru Takeda

{"title":"Novel gelling agent similar to galactomannan from the sheath-forming bacterium Sphaerotilus hippei.","authors":"Mishal Shafiq, Shiqian Chen, Naoki Matsunaga, Aoi Hagio, Ichiro Suzuki, Daisuke Koizumi, Rie Togawa, Yudai Yamaoki, Keiko Kondo, Masato Katahira, Minoru Takeda","doi":"10.1093/jambio/lxaf220","DOIUrl":"10.1093/jambio/lxaf220","url":null,"abstract":"Aims: Hydrogels have applications as food additives and cosmetics, as well as medical applications such as the drug delivery and scaffolding materials for cells. There is a high demand for new hydrogels that can facilitate technological innovation. Here, we report a galactomannan-like gelling agent (hydrogel) produced by the sheath-forming bacterium Sphaerotilus hippei.Methods and results: Sphaerotilus hippei was grown on glucose and tuna extract. The presence of a microtube-shaped sheath with a closed end was confirmed using electron microscopy. The sheath was prepared by treatment with sodium dodecyl sulphate and NaOH. Chemical composition analysis and nuclear magnetic resonance spectroscopy revealed that the sheath is an assemblage of a polysaccharide with a tetrasaccharide repeating unit composed of β-d-Galp, α-d-Manp, and β-d-Manp in the molar ratio of 2:1:1. Molecular simulations suggested a right-handed helical structure with 16 sugar residues per turn and a pitch of 4.0-5.0 Å. A hydrogel was formed by solubilization of the sheath in hot water and subsequent cooling.Conclusions: The sheath of S. hippei is a closed-ended microtube assembled with galactomannan-like polysaccharide, which may have a helical conformation. The sheath has hydrogel-forming capabilities.","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144955318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pyrogallol exhibits antimicrobial adjuvant properties against Staphylococcus aureus and Staphylococcus epidermidis. 邻苯三酚具有抗金黄色葡萄球菌和表皮葡萄球菌的抗菌佐剂特性。

IF 3.2 3区生物学

Journal of Applied Microbiology Pub Date : 2025-08-04 DOI: 10.1093/jambio/lxaf189

Katharina H C Roese, Sarah Ibrahim, Lauren A Cooper, Afnan S Yahya, Dongjae Kang, Nikhil R Tirupathigari, Koby A J LaRose, Karen L Houseknecht, Deborah J Barlow, Kristin M Burkholder

{"title":"Pyrogallol exhibits antimicrobial adjuvant properties against Staphylococcus aureus and Staphylococcus epidermidis.","authors":"Katharina H C Roese, Sarah Ibrahim, Lauren A Cooper, Afnan S Yahya, Dongjae Kang, Nikhil R Tirupathigari, Koby A J LaRose, Karen L Houseknecht, Deborah J Barlow, Kristin M Burkholder","doi":"10.1093/jambio/lxaf189","DOIUrl":"10.1093/jambio/lxaf189","url":null,"abstract":"Aims: To investigate the effect of pyrogallol as an antimicrobial adjuvant against staphylococci.Methods and results: We tested the effect of pyrogallol on the susceptibility of four strains of Staphylococcus aureus and S. epidermidis to antibiotics. In minimum inhibitory concentration (MIC) assays, pyrogallol significantly enhanced the anti-staphylococcal efficacy of linezolid against all strains and increased cefoxitin and oxacillin activity against individual strains. Given pyrogallol's prooxidant properties, we compared its effect on linezolid activity in wild-type and catalase-deficient S. aureus. Catalase-deficient bacteria exhibited greater linezolid sensitivity than wild-type (WT) in presence of pyrogallol. Antibiotic accumulation assays revealed greater intracellular linezolid concentration in S. aureus treated with pyrogallol compared to bacteria treated with linezolid alone.Conclusions: Pyrogallol potentiates linezolid-mediated killing of staphylococci in a manner involving oxidative stress and intra-bacterial antibiotic accumulation. Pyrogallol may have utility as an anti-staphylococcal antimicrobial adjuvant.","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144731104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Temporal shift of bacterial communities in poultry litter during the course of broiler chicken rearing: a 16S rRNA-based metagenomic study. 肉鸡饲养过程中鸡窝细菌群落的时间变化：基于16S rRNA的宏基因组研究

IF 3.2 3区生物学

Journal of Applied Microbiology Pub Date : 2025-08-04 DOI: 10.1093/jambio/lxaf199

Embar Prasanna Kannan, Prasanth Venkatachalam, Judy Gopal, Ponmariappan Sarkaraisamy, Manikandan Muthu

{"title":"Temporal shift of bacterial communities in poultry litter during the course of broiler chicken rearing: a 16S rRNA-based metagenomic study.","authors":"Embar Prasanna Kannan, Prasanth Venkatachalam, Judy Gopal, Ponmariappan Sarkaraisamy, Manikandan Muthu","doi":"10.1093/jambio/lxaf199","DOIUrl":"10.1093/jambio/lxaf199","url":null,"abstract":"Aim: To decipher the bacterial community transitions of poultry litter at various time frames over a 6-week rearing cycle in a commercial broiler chicken poultry farm in Marakanam, Tamil Nadu, India.Methods and results: The bacterial consortia of poultry litter were elucidated using the 16S rRNA-based metagenomic Oxford nanopore sequencing method, followed by taxonomic assignment using the Kraken2 tool. Our findings unveiled the varied dominance patterns of bacteria in poultry litter (P1-P6) with Sphingobacterium sp. 21 (53%) in P1, Amphibacillus xylanus dominated in P2 (∼12%), Oceanimonas sp. GK1 in P3 (∼14%) and P6 (>50%), Anaerococcus prevotii in P4 (∼10%) and Marinobacter hydrocarbonoclasticus (∼15%) in P5. Importantly various bacteria involved in key biogeochemical cycles, including Nitrosomonas eutropha (nitrogen cycle), Bacillus subtilis (phosphorous cycle), and Acidithiobacillus caldus (sulphur cycle) were detected indicating a strong potential of utilizing the poultry litter as an effective biofertilizer. On the other hand, harmful human pathogens, including Mycobacterium tuberculosis, Escherichia coli, Salmonella enterica, and Streptococcus pneumoniae were also detected indicating significant public health concern associated with the application of poultry litter as fertilizer.Conclusions: This study offers knowledge to both leverage the usefulness of poultry litter as biofertilizer and to mitigate transmission of harmful pathogens harboured by poultry litter.","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144835173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sludge-to-gas: aquaculture fish sludge shows varying hydrogen production potential over the lifetime of a fish. 污泥制气：水产养殖鱼类污泥在鱼的一生中显示出不同的产氢潜力。

IF 3.2 3区生物学

Journal of Applied Microbiology Pub Date : 2025-08-04 DOI: 10.1093/jambio/lxaf188

Biwen Annie An-Stepec, Melanie Andrews, Abduljelil Kedir, Natalie Schindler, Nicole Dopffel

{"title":"Sludge-to-gas: aquaculture fish sludge shows varying hydrogen production potential over the lifetime of a fish.","authors":"Biwen Annie An-Stepec, Melanie Andrews, Abduljelil Kedir, Natalie Schindler, Nicole Dopffel","doi":"10.1093/jambio/lxaf188","DOIUrl":"10.1093/jambio/lxaf188","url":null,"abstract":"Aims: The fish industry is growing, with millions of tons of fish cultured in off-land and on-land aquaculture systems. A major challenge is the continuous production of sludge containing fish secretions and uneaten fish feed. Anaerobic digestion (AD) can efficiently use this sludge, involving biological degradation of organic matter by microbes under anoxic conditions, forming valuable biogases like methane. Recently, there is an increasing need for green hydrogen (H2), but the potential of using fish sludge for H2 production has not been fully explored. A key complicating factor is the changing sludge composition during a fish's lifetime.Methods and results: Salmon fish sludge was sampled from a land-based recirculating aquaculture system (RAS) over 6 months, covering fresh, brackish, and seawater stages. Sludge was used for AD batch incubation to investigate H2 and liquid compound productivity. Chemical- and DNA-based microbial community analysis of the sludge showed significant changes over time. This was also reflected in changing ad productivity. Highest H2 production (24.5 ± 17.5 mL H2 gas per 1-g dry sludge) was within the freshwater phase, followed by brackish. Concurrently in all enrichments we observed high production of CO2 (75.3 ± 30.1 mL CO2 per 1 g of dry sludge) and volatile fatty acids, especially acetic acid, propionic acid, and lactic acid.Conclusions: The microbial community in fish sludge can naturally produce H2 and other valuable products, with productivity varying over the fish's lifetime. The key governing factor for H2 production is the type of fish sludge and availability of electron acceptors.","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144690362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0