Journal of Applied Microbiology最新文献

{"title":"Bioprospecting endophytic fungi of forest plants for their monoamine oxidase A and cholinesterases inhibitors, and peroxisome proliferator-activated receptor gamma agonists.","authors":"El-Sayed R El-Sayed, Joanna Grzelczyk, Tomasz Strzała, Ilona Gałązka-Czarnecka, Grażyna Budryn, Filip Boratyński","doi":"10.1093/jambio/lxaf034","DOIUrl":"10.1093/jambio/lxaf034","url":null,"abstract":"<p><strong>Aims: </strong>The urgent search for new natural bioactive compounds is crucial to address growing clinical demands. With this perspective, this paper focuses on isolating and bioprospecting fungal endophytes from some plant species in a local forest in Wrocław, Poland.</p><p><strong>Methods and results: </strong>Forty-three fungal endophytes were isolated and their extracts were tested for inhibitory potential against monoamine oxidase A (MAO-A), acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and for peroxisome proliferator-activated receptor gamma (PPAR-γ) agonists. Six promising strains after screening were identified to possess all these activities. These strains and their respective plant hosts were Sphaeropsis sapinea BUK-L2 (Fagus sylvatica), Coniochaeta velutina SW-B (Picea abies), Epicoccum nigrum COR-B (Corylus avellana), Paraphaeosphaeria verruculosa JAR-B (Sorbus aucuparia), Umbelopsis isabellina COR-L1 (Corylus avellana), and Epicoccum mezzettii QR-B (Quercus robur). Moreover, gamma irradiation at several doses (Gy) was separately applied to the fungal cultures to study their enhancement effects on the recorded activities. Finally, compounds of active bands from preparative thin-layer chromatography of the two promising strains (Coniochaeta velutina SW-B and Epicoccum nigrum COR-B), were identified by GC-MS (Gas chromatography-mass spectrometry).</p><p><strong>Conclusions: </strong>The present study is the first report on bioprospecting endophytic fungi of forest plants for the aforementioned activities.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143413228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deaminase inhibitor and casein hydrolysates drive microbial shifts favoring Campylobacter jejuni in an in vitro poultry cecal model.","authors":"Elena G Olson, Chamia C Chatman, Dana K Dittoe, Erica L-W Majumder, Hilario C Mantovani, Steven C Ricke","doi":"10.1093/jambio/lxaf046","DOIUrl":"10.1093/jambio/lxaf046","url":null,"abstract":"<p><strong>Aim: </strong>The dietary proteins in poultry feeds, including the polypeptide chain size, influence gut microbial composition and function. This study assessed the microbial preference for peptide size using the same protein source in three polypeptide forms.</p><p><strong>Methods and results: </strong>We investigated the effects of diphenyliodonium chloride (DIC) on poultry cecal microbiota inoculated with Campylobacter jejuni and supplemented with various casein hydrolysates (intact casein, enzyme hydrolysate, acid hydrolysate, and a mix of all three) using an in vitro cecal model. The incubation occurred over 18 h at 42°C under microaerophilic conditions. We hypothesized a decrease in C. jejuni abundance by limiting nitrogenous metabolites while promoting the growth of protein fermentative bacteria. Additionally, we speculated that the response to DIC would vary with different polypeptides. Genomic DNA was extracted, amplified, and sequenced on an Illumina MiSeq platform. Analysis within QIIME2-2021.11 showed that DIC treatments did not significantly affect C. jejuni abundance but drastically decreased Enterobacteriaceae abundance (ANCOM, P < 0.05). DIC-treated groups exhibited a more stable community structure, especially in the peptide-amended group. Microbial interactions likely aided C. jejuni survival in DIC groups with casein hydrolysates. Methanocorpusculum, Phascolarctobacterium, and Campylobacter formed a core microbial community in both DIC-treated and non-treated groups. DIC altered co-occurrence patterns among core members and differentiated taxa in abundance in acid and peptide-DIC treated groups, changing negative relationships to positive ones (Spearman's Correlation, P < 0.05). Variations in polypeptide composition affected metabolite abundance, notably impacting the urea cycle in Campylobacter and Clostridiaceae. DIC shifted communal energy metabolism in microbiota on casein sources.</p><p><strong>Conclusion: </strong>Campylobacter's adaptability to the deaminase inhibitor indicates reliance on the microbial community and their metabolic products, showcasing its metabolic versatility.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143556933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of Pseudomonas phage MME: a novel tool for combatting multidrug-resistant Pseudomonas aeruginosa and disinfection.","authors":"Berna Erdogdu, Tulin Ozbek","doi":"10.1093/jambio/lxaf052","DOIUrl":"10.1093/jambio/lxaf052","url":null,"abstract":"<p><strong>Aim: </strong>Combatting Pseudomonas aeruginosa, known for its robust biofilm formation, presents significant challenges in healthcare, food, and industry. Phages offer promising alternatives against this resilient pathogen. We aim to demonstrate their viability as alternative therapeutic and decontamination options.</p><p><strong>Methods and results: </strong>We introduce the lytic activity and decontamination efficacy of Pseudomonas phage MME, isolated from sewage, on solid surfaces, as well as on its biological and genomic characterization. The phage showed lytic activity against both antibiotic-resistant clinical strains and reference strains. About 90% of the phage adsorbed to its host within 20 min, with an average burst size of ∼53 PFU per infected cell. The bactericidal effect on the host at the 8th hour showed a 95% killing efficiency. Additionally, phage MME effectively reduced bacterial loads on glass, plastic, and metal surfaces, simulating hospital environments. Confocal laser scanning microscopy demonstrated the phage's bactericidal activity on glass surfaces at the 8th and 12th hours, preventing biofilm formation. Bioinformatic analysis confirmed that phage MME represents a new species within the Bruynoghevirus genus. Comparative genomic analysis revealed no virulence factors within the phage MME genome.</p><p><strong>Conclusions: </strong>These findings highlight the potent lytic activity of phage MME against P. aeruginosa, underscoring its potential as a valuable tool in combatting this pathogen and its suitability for diverse applications, including as a decontaminating agent.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143567216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pseudomonas aeruginosa: One Health approach to deciphering hidden relationships in Northern Portugal.","authors":"Telma de Sousa, Sandro Machado, Manuela Caniça, Miguel J N Ramos, Daniela Santos, Miguel Ribeiro, Michel Hébraud, Gilberto Igrejas, Olimpia Alves, Eliana Costa, Augusto Silva, Ricardo Lopes, Patrícia Poeta","doi":"10.1093/jambio/lxaf037","DOIUrl":"10.1093/jambio/lxaf037","url":null,"abstract":"<p><strong>Aims: </strong>Antimicrobial resistance in Pseudomonas aeruginosa represents a major global challenge in public and veterinary health, particularly from a One Health perspective. This study aimed to investigate antimicrobial resistance, the presence of virulence genes, and the genetic diversity of P. aeruginosa isolates from diverse sources.</p><p><strong>Methods and results: </strong>The study utilized antimicrobial susceptibility testing, genomic analysis for resistance and virulence genes, and multilocus sequence typing to characterize a total of 737 P. aeruginosa isolates that were collected from humans, domestic animals, and aquatic environments in Northern Portugal. Antimicrobial resistance profiles were analyzed, and genomic approaches were employed to detect resistance and virulence genes. The study found a high prevalence of multidrug-resistant isolates, including high-risk clones such as ST244 and ST446, particularly in hospital sources and wastewater treatment plants. Key genes associated with resistance and virulence, including efflux pumps (e.g. MexA and MexB) and secretion systems (T3SS and T6SS), were identified.</p><p><strong>Conclusions: </strong>This work highlights the intricate dynamics of multidrug-resistant P. aeruginosa across interconnected ecosystems in Northern Portugal. It underscores the importance of genomic studies in revealing the mechanisms of resistance and virulence, contributing to the broader understanding of resistance dynamics and informing future mitigation strategies.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143414107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fecal indicator bacteria and sewage-associated marker genes are associated with nitrate and environmental parameters in two Florida freshwater systems.","authors":"Amanda M Brandt, Jacob K Senkbeil, Aldo E Lobos, Carley Defillips, David B Lewis, Valerie J Harwood","doi":"10.1093/jambio/lxaf030","DOIUrl":"10.1093/jambio/lxaf030","url":null,"abstract":"<p><strong>Aim: </strong>Identify relationships among microbial variables [fecal indicator bacteria (FIB) and microbial source tracking (MST) marker genes] and nutrients to improve source identification in two polluted, freshwater streams in Florida.</p><p><strong>Methods and results: </strong>Water and sediment were sampled at Bullfrog Creek (BFC) and Sweetwater Creek (SWC), which varied in land use and expected sources of fecal pollution. Escherichia coli and enterococci were cultured, sewage-associated HF183 and bird-associated GFD genetic markers were assessed by quantitative polymerase chain reaction (qPCR), and organic and inorganic nutrients were analyzed. Escherichia coli and enterococci exceeded recreational water quality criteria in 47% and 85% of samples, respectively, at less urbanized BFC, and in 8% and 62% of samples at more urbanized SWC. HF183, but not GFD, was positively associated with surface water nitrate by multivariate analysis and binary logistic regression. Sediment organic matter was lowest at urbanized sites in both streams and inversely associated with surface water FIB.</p><p><strong>Conclusions: </strong>Measuring nutrients alongside FIB and MST revealed that nitrate levels in water, but not phosphorus or organic carbon levels, were predictive of sewage pollution.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143382367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sulfate-reducing bacteria block cadmium and lead uptake in rice by regulating sulfur metabolism.","authors":"Li-Rong Fang, Jing-Yu Ren, Kai Sun, Wei Zhang, Wei He, Chuan-Chao Dai","doi":"10.1093/jambio/lxaf022","DOIUrl":"10.1093/jambio/lxaf022","url":null,"abstract":"<p><strong>Aim: </strong>This study was dedicated to investigating the role of sulfur metabolic processes in sulfate-reducing bacteria in plant resistance to heavy metal contamination.</p><p><strong>Methods and results: </strong>We constructed sulfate-reducing bacterial communities based on the functional properties of sulfate-reducing strains and then screened out the most effective sulfate-reducing bacterial community SYN1, that prevented Cd and Pb uptake in rice through a hydroponic experiment. This community lowered Cd levels in the roots and upper roots by 36.60% and 39.88%, respectively, and Pb levels by 35.96% and 51.54%. We also compared two treatment groups, inoculated with SYN1 and exogenously added GSH, and found that both enhanced the antioxidant response of the plants, increased the lignin and GSH contents and the expression of genes related to the phenylpropane biosynthesis pathway (OsCAD, Os4CL, OsCOMT, OsPOD, OsC3H, and OsPAL), and decreased the expression of heavy metal transporter genes (OsHMA2, OsIRT1) expression. There were no significant differences between the two treatments.</p><p><strong>Conclusions: </strong>Sulfate-reducing bacteria produce GSH through the sulfur assimilation pathway, and GSH can directly chelate heavy metals or enhance plant antioxidant enzyme activities and regulate processes such as the uptake and translocation of heavy metals, thus enhancing plant resistance to heavy metal toxicity.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143052558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Using bacterial and mitochondrial DNA markers to assess fecal pollution sources in stream water and sediments of a mixed land-use watershed.","authors":"Wenjing Ren, Eve F Brantley, Xu Wang, Joan B Rose, Yucheng Feng","doi":"10.1093/jambio/lxaf021","DOIUrl":"10.1093/jambio/lxaf021","url":null,"abstract":"<p><strong>Aims: </strong>Although stream bed sediment can be an important reservoir of fecal bacteria and pathogens, it is rarely analyzed when assessing microbial water quality. This study aims to identify fecal contamination sources in stream water and sediment and evaluates the impact of rainfall events on microbial water quality in a mixed land-use watershed.</p><p><strong>Methods and results: </strong>Quantitative polymerase chain reaction was employed to quantify human-, cattle-, and chicken-associated genetic markers during dry and wet periods in the Middle Tallapoosa watershed in Alabama, USA. Human- and cattle-associated markers were consistently detected in water samples, irrespective of precipitation, whereas chicken-associated markers were predominantly found following significant rainfall events. In the sediment, all markers were detected at higher concentrations but with a lower frequency than in the water. Escherichia coli concentrations in water samples correlated significantly with 2-day antecedent rainfall and streamflow and were substantially lower than in the sediment.</p><p><strong>Conclusions: </strong>Humans and cattle were the primary contamination sources in the study area, and runoff from storms and sediment contributed to fecal contamination in the streams.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143074641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of antimicrobial resistance phenotypes and genes in stable fly- and manure-derived bacterial isolates from clinically relevant taxa in dairy settings.","authors":"Andrew J Sommer, Julia E Kettner, Travis K Worley, Jordan Petrick, Caroline Haynie, Kerri L Coon","doi":"10.1093/jambio/lxaf025","DOIUrl":"10.1093/jambio/lxaf025","url":null,"abstract":"<p><strong>Aims: </strong>This study aimed to characterize and compare the antimicrobial resistance profiles of clinically relevant bacterial taxa isolated from biting stable flies (Stomoxys spp.) and bovine manure samples collected at a dairy research facility over the course of an entire fly breeding season. The presence of extended-spectrum beta-lactamase and other antimicrobial resistance genes (ARGs) was also examined.</p><p><strong>Methods and results: </strong>A total of 606 fly- and 180 manure-derived strains were tested via disk diffusion for susceptibility to commonly administered antibiotics used in veterinary and human medicine. A small percentage of Enterobacterales exhibited resistance to the tested antimicrobials, including ceftiofur and other beta-lactam antibiotics. Extended spectrum beta-lactamase genes (TEM, CTX, OXA, CMY) were detected by polymerase chain reaction (PCR) amplification in ceftiofur-resistant Escherichia coli, Klebsiella, and Enterobacter spp. isolates. We additionally identified pirlimycin-resistant Staphylococcus and Mammaliicoccus spp. isolates encoding lnuA, a lincosamide resistance gene found primarily on small mobilizable plasmids.</p><p><strong>Conclusions: </strong>These findings highlight the significance of stable flies in the carriage of antimicrobial-resistant bacterial strains and plasmid-associated ARGs on dairy farms.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143065813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrophobic 2-methacryloyloxyethyl phosphorylcholine polymer inhibits peri-implantitis-causing bacterial adhesion on titanium materials.","authors":"Minato Akizuki, Keiji Murakami, Kazumitsu Sekine, Akikazu Murakami, Koh Kobayashi, Masaru Matsuda, Haruka Matsumoto, Eiji Harata, Kenichi Hamada, Raras Ajeng Enggardipta, Hideki Fujii, Hiromichi Yumoto","doi":"10.1093/jambio/lxaf033","DOIUrl":"10.1093/jambio/lxaf033","url":null,"abstract":"<p><strong>Aims: </strong>To prevent peri-implantitis, we investigated the adhesion of periodontopathogenic bacteria to titanium surfaces using a hydrophobic 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer to inhibit adhesion.</p><p><strong>Method and results: </strong>We immersed titanium plates (TiPs) coated with a hydrophobic MPC polymer in a bacterial suspension for 30 min or 24 h and measured the number of adherent bacteria. Bacteria adhering to the TiPs were observed using scanning electron microscopy (SEM). Furthermore, mimicking an oral cavity, TiPs coated with MPC polymer and saliva, were immersed in bacterial suspensions of Porphyromonas gingivalis, Fusobacterium nucleatum, and Streptococcus mutans for 24 h, and adenosine triphosphate in the adherent bacteria was measured.Bacterial adhesion was significantly inhibited on MPC polymer-coated TiPs after 30 min and 24 h. SEM results showed a similar trend. Bacterial adhesion was significantly inhibited on MPC polymer-treated TiPs in the presence of saliva, both before and after MPC treatment. Furthermore, their effectiveness was maintained when the MPC polymer-treated TiPs were stored in saline for 1 week.</p><p><strong>Conclusions: </strong>Hydrophobic MPC polymer coating on TiP surface inhibited bacterial adhesion, indicating that it may be effective in preventing peri-implantitis.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143440856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combating enteropathogenic and multidrug resistant Escherichia coli using the lytic bacteriophage vB_EcoM_ECO78, which disrupts bacterial biofilm formation and exhibits a remarkable environmental stability.","authors":"Azza A H Rajab, El-Sayed Khafagy, Amr S Abu Lila, Nehal Yousef, Momen Askoura","doi":"10.1093/jambio/lxaf028","DOIUrl":"10.1093/jambio/lxaf028","url":null,"abstract":"<p><strong>Aim: </strong>The current study aimed to establish a phenotypic and genotypic characterization record of a novel lytic bacteriophage (phage) against multidrug-resistant (MDR) Escherichia coli (E. coli) infections.</p><p><strong>Methods and results: </strong>Phenotypic characterization of the isolated phage included the assessment of phage morphology, host range, stability, and antibiofilm activity. The isolated phage vB_EcoM_ECO78 demonstrated a high lytic activity against MDR E. coli and E. coli serotypes O78: K80: H12 and O26: H11. Additionally, it showed a marked antibiofilm activity and high physical stability at a wide range of temperatures and pH. Genotypic investigations identified a double-stranded DNA genome of 165 912 base pairs (bp) spanning 258 open reading frames (ORFs), out of which 149 ORFs were identified and annotated. In vivo analysis further confirmed the therapeutic potential of vB_EcoM_ECO78 which effectively increased the survival of mice infected with MDR E. coli.</p><p><strong>Conclusion: </strong>The isolated phage vB_EcoM_ECO78 exhibits considerable stability and antibiofilm activity against MDR E. coli isolates, supported by notable environmental fitness and in vivo antibacterial capability.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143370641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}