Journal of Applied Microbiology最新文献

{"title":"Lactobacillus acidophilus (strain Scav) postbiotic metabolites reduce infection and modulate inflammation in an in vivo model of Pseudomonas aeruginosa wound infection.","authors":"Rachael M Wilson, Jean M Walker, Joris Beld, Kingsley Yin","doi":"10.1093/jambio/lxaf061","DOIUrl":"10.1093/jambio/lxaf061","url":null,"abstract":"<p><strong>Aims: </strong>This study assessed the antibacterial, antibiofilm, and immunomodulatory activity of Lactobacillus acidophilus (strain Scav) postbiotic (LaP) in a mouse model of Pseudomonas aeruginosa wound infection and evaluated the bioactive components of the LaP.</p><p><strong>Methods and results: </strong>LaP was tested for Pseudomonas aeruginosa clearance and immunomodulatory activity during wound infection. We show that LaP applied 1 h after infection reduced tissue bacterial burden within 24 h, and this reduction persisted for 5 days. Ciprofloxacin given once at the exact same time did not reduce bacteria load as compared to vehicle controls. LaP reduced plasma IL-6 and MCP-1 levels after 5 days. Wound tissue IL-6 and MCP-1 levels were increased in infected vehicle mice at 5 days, but tissues from LaP-treated mice were similar to sham controls. LaP increased tissue IL-10 (antiinflammatory cytokine) levels. Ciprofloxacin decreased plasma and tissue IL-6 compared to vehicle controls but did not affect MCP-1 or IL-10 levels. To elucidate antibacterial and antibiofilm metabolite(s) in LaP, fractionation followed by Ps. aeruginosa antagonistic activity assays were performed. This was followed by liquid chromatography coupled to mass spectrometry (LCMS) analysis. Our analyses identified a low molecular weight, polar molecule, which had both antibacterial and antibiofilm activity.</p><p><strong>Conclusions: </strong>Lactobacillus acidophilus secretes an antibacterial and antibiofilm metabolite that reduced pathogen burden and resolved systemic inflammation in a Pseudomonas aeruginosa wound infection model.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11951090/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143604829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to: Evaluation of the long-term protection conferred by an organosilicon-based disinfectant formulation against bacterial contamination of surface.","authors":"","doi":"10.1093/jambio/lxaf069","DOIUrl":"https://doi.org/10.1093/jambio/lxaf069","url":null,"abstract":"","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":"136 3","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143742851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A systematic review on the utility of wastewater surveillance for monitoring yellow fever virus and other arboviruses.","authors":"Caleb Morin, Zina Alfahl","doi":"10.1093/jambio/lxaf066","DOIUrl":"10.1093/jambio/lxaf066","url":null,"abstract":"<p><strong>Aims: </strong>This review aims to examine wastewater surveillance for the detection of yellow fever virus (YFV) and related arboviruses, focusing on concentration and extraction methodology, viral decay kinetics, and quantification techniques.</p><p><strong>Methods: </strong>A literature search was conducted across 5 databases: PubMed, Science Direct, Web of Science, Embase, and Google Scholar following the PRISMA guidelines. Studies included were original scientific articles published between April 2014 and April 2024. Human research studies investigating wastewater surveillance and YFV or other arboviruses/flaviviruses were assessed.</p><p><strong>Results: </strong>A total of 17 studies were included in this review. YFV was not detected in population-based wastewater samples; however, successful detection of similar viruses suggests potential for YFV monitoring with wastewater surveillance. YFV-spiked wastewater studies reveal similar concentration efficiency and decay rates between arboviruses. Effective concentration methods for YFV likely include centrifugation ultrafiltration and solid pellet extraction. YFV and arboviruses decay faster at higher temperatures, though YFV remains detectable for several days at these temperatures.</p><p><strong>Conclusions: </strong>Wastewater surveillance presents a promising approach for monitoring YFV and other arboviruses. However, further research is needed to overcome existing limitations and enhance its effectiveness.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143648646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Humic substances modulate bacterial communities and mitigate adverse effects of temperature stress in coral reef organisms.","authors":"Tamara M Stuij, Daniel F R Cleary, Nicole J de Voogd, Rui J M Rocha, Ana Rita M Polónia, Davide A M Silva, Jörg C Frommlet, Antonio Louvado, Yusheng M Huang, Newton C M Gomes","doi":"10.1093/jambio/lxaf024","DOIUrl":"10.1093/jambio/lxaf024","url":null,"abstract":"<p><strong>Aims: </strong>In the present study, we tested whether terrestrially derived humic substances (HS) could mitigate the adverse effects of elevated temperature and ultraviolet B (UVB) radiation on the bacterial communities of two hard corals (Montipora digitata and M. capricornis), one soft coral (Sarcophyton glaucum), sediment and water. We also examined the impact of temperature, UVB radiation, and HS supplementation on coral photosynthetic activity, a proxy for coral bleaching.</p><p><strong>Methods and results: </strong>We performed a multifactorial experiment using a randomized-controlled microcosm setup. Coral photosynthetic efficiency was measured in vivo using a pulse amplitude modulation fluorometer. Bacterial communities were analyzed using 16S rRNA gene sequencing. Corals in HS-supplemented microcosms had significantly higher photosynthetic activities than those in microcosms subjected to elevated temperature and UVB radiation. Additionally, HS supplementation significantly influenced the composition of sediment, water, and host-associated bacterial communities. Reef organisms in HS supplemented microcosms contained distinct bacterial communities enriched with groups of potentially beneficial bacteria. In the hard coral M. digitata, we observed an interactive effect of HS supplementation, UVB radiation, and temperature.</p><p><strong>Conclusion: </strong>Our findings indicate that HS significantly modulates coral reef bacterial communities and support the hypothesis that these substances contribute to improved reef resistance to the adverse effects of elevated temperature and UVB radiation.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143059153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptional analysis provides molecular insights into degeneration of the edible fungus Flammulina filiformis.","authors":"Caiyi Wang, Yu Sun, Xinyu Yang, Zehao Wang, Shibo Xiang, Zhuqing Huang, Yue Liang","doi":"10.1093/jambio/lxaf039","DOIUrl":"10.1093/jambio/lxaf039","url":null,"abstract":"<p><strong>Aims: </strong>Flammulina filiformis, a widely cultivated edible fungus, frequently suffers from strain degeneration, leading to reduce yield and quality, and causing significant economic losses in large-scale production. This study aimed to better understand degeneration mechanisms and to develop an assay for predicting degeneration.</p><p><strong>Methods and results: </strong>This study investigates strain degeneration by comparing a cultivated strain (F1) and its subcultured derivative (F7). Although both strains display similar mycelial growth, F7 shows impaired fruiting body production, indicating that degeneration occurs prior to visible changes in mycelial growth. To uncover the molecular mechanisms underlying degeneration, transcriptome analysis was carried out. A total of 352 down-regulated and 280 up-regulated differentially expressed genes (DEGs) were identified in F7 compared to F1. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses revealed significant enrichment of DEGs in functional categories related to oxidoreductase activities and peroxisome pathway. Quantitative PCR further validated the regulation of certain DEGs associated with these enriched functions. A stress tolerance assay was developed to detect degeneration in strains with unchanged mycelial growth but reduced fruiting body production.</p><p><strong>Conclusions: </strong>Results suggested that strain degeneration in F. filiformis is closely linked to oxidative stress regulation and occurs prior to observable mycelial impairment.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143468177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salmonella Infantis outbreak on six broiler units in Great Britain: investigation, epidemiology, and control.","authors":"Shaun Cawthraw, Andrew Wales, Jaromir Guzinski, Jahcub Trew, Isaac Ring, Tom Huby, Arslan Hussaini, Liljana Petrovska, Francesca Martelli","doi":"10.1093/jambio/lxaf040","DOIUrl":"10.1093/jambio/lxaf040","url":null,"abstract":"<p><strong>Aims: </strong>To describe the analysis, epidemiology, and control of six contemporaneous and linked outbreaks of Salmonella enterica subsp. enterica serovar Infantis on British broiler farms. Salmonella Infantis is a potentially multidrug-resistant foodborne zoonosis and can persistently colonize poultry flocks and farms.</p><p><strong>Methods and results: </strong>Routine monitoring initially identified the organism, which was tracked to six farms associated with a single company. Extensive, repeat sampling identified widespread and, in some cases, persistent contamination. Salmonella Infantis was also isolated from three associated processing factories and catching crew equipment, but not from associated hatcheries and feed mills. Whole genome sequencing and resistance phenotyping revealed one strain was present in the processing plants and on five farms. However, on one of those farms, several highly genetically distinct strains were also detected, including one also found in one of the processing plants. The sixth farm had a strain that was genetically unrelated to strains collected from the other premises and which exhibited an extended spectrum beta-lactamase phenotype. Cleaning and disinfection were enhanced, and the organism was eventually cleared from all farms.</p><p><strong>Conclusions: </strong>There were multiple incursions of varied strains, with a possible link to processing factories. Elimination of S. Infantis from premises can be challenging but achievable.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143476654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating genomic, transcriptomic, and phenotypic information to explore drug resistance in Mycobacterium tuberculosis sub-lineage 4.2.2.2.","authors":"Tesfaye Gebreyohannis Hailemariam, Abaysew Ayele, Tesfaye Gelanew, Abay Atnafu, Michael Brennan, Melaku Tilahun, Dawit Hailu Alemayehu, Zemedkun Abebe Debella, Yared Merid, Workineh Shibeshi, Abraham Aseffa, Kidist Bobosha, Yonas Hirutu, Simon J Waddell, Ephrem Engidawork","doi":"10.1093/jambio/lxaf063","DOIUrl":"10.1093/jambio/lxaf063","url":null,"abstract":"<p><strong>Aims: </strong>Mycobacterium tuberculosis (Mtb) remains a major global health challenge, particularly due to increasing drug resistance. Beyond the well-characterized mutations, the mechanisms involved in driving resistance appear to be more complex. This study investigated the differential gene expression of Ethiopian drug-resistant Mtb sub-lineage 4.2.2.2 clinical isolates through an integrated approach combining phenotypic, transcriptomic, and genomic analyses.</p><p><strong>Method and results: </strong>RNA sequencing was performed by isolating RNA from six Mtb strains (three drug-sensitive and three drug-resistant) during mid-logarithmic phase growth. Drug resistance was assessed through whole-genome analysis and phenotypic testing using the BACTEC Mycobacteria growth indicator tube (MGIT)™ 960 system. RNA profiling revealed significantly reduced expression of six genes: Rv0096, Rv2780, Rv3136, Rv3136A, Rv3137, and Rv3230c in drug-resistant isolates. These genes are not associated with known drug targets nor resistance mechanisms. Additionally, a discrepancy was noted between phenotypic resistance profiles and whole genome-based predictions, with the latter suggesting broader resistance. For instance, the missense mutation in rpoB p.Ser450Leu and katG p.Ser315Thr were identified with no change in phenotypic drug sensitivity to rifampicin and isoniazid, respectively.</p><p><strong>Conclusion: </strong>Identification of these differentially expressed genes and their networks could be useful in unraveling the complexities of Mtb drug resistance and in understanding the impact that drug resistance conferring mutations have on the physiology of drug-resistant Mtb.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11940716/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143615612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Virucidal activity of household laundry detergents and additives in simulated wash cycles.","authors":"Justyna E Konkol, Britta Becker, Dajana Paulmann, Eike Steinmann, Daniel Todt, Toni L Meister, Stefan Evers, Andreas Dotzauer, Mirko Weide, Florian H H Brill","doi":"10.1093/jambio/lxaf057","DOIUrl":"10.1093/jambio/lxaf057","url":null,"abstract":"<p><strong>Aims: </strong>In this study, we evaluated the antiviral performance of domestic laundry detergents and additives to provide information for selecting a suitable product for reducing viruses on textiles during viral infections at home.</p><p><strong>Methods and results: </strong>Three laundry heavy-duty detergents (HDDs)-universal solid, regular liquid, and compact single-dose liquid-and a laundry additive (hygiene rinser) were tested for their efficacy using a suspension test and lab-scale laundering against two viruses: enveloped modified vaccinia virus Ankara (MVA) and nonenveloped murine norovirus (MNV). All HDD detergents effectively inactivated MVA at 30°C (titers reduced below quantification limit), whereas the hygiene rinser at 20°C exhibited lower efficacy, with 2.19 log10 reduction after 30 min. Notably, at low temperatures, HDD solid effectively reduced the MNV titer (5 log10 reduction after 30 min).</p><p><strong>Conclusions: </strong>Laundry detergents, including HDD liquid detergents, can inactivate MVA at 30°C. However, for complete inactivation of nonenveloped viruses like norovirus, detergents containing bleach, like the universal solid, are required.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143648738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the resistance: comparative genomic analysis of two novel cefiderocol-resistant Stenotrophomonas species from a referral hospital in Mexico City.","authors":"Jossue Ortiz Álvarez, Corazón Barrientos Flores, Claudia Adriana Colín Castro, Melissa Hernández Durán, María Guadalupe Martínez Zavaleta, Braulio Josué Méndez Sotelo, Cindy Fabiola Hernández Pérez, Christian Sohlenkamp, Rafael Franco Cendejas, Luis Esaú López Jácome","doi":"10.1093/jambio/lxaf048","DOIUrl":"10.1093/jambio/lxaf048","url":null,"abstract":"<p><strong>Background: </strong>Stenotrophomonas maltophilia is the species most frequently identified by clinical microbiology laboratories due to its presence in the main identification systems databases. Phenotypic identification methods are widely used in laboratories, and the misidentification of Stenotrophomonas spp. is highly probable due to the presence of cryptic species. Our aim was to confirm the identity of five cefiderocol-resistant Stenotrophomonas species, initially identified as S. maltophilia, using genome analysis tools, performing comparative and functional analyses of these clinical strains associated with infectious processes.</p><p><strong>Methods: </strong>Identifications were performed using average nucleotide identity, average amino acid identity, and in silico DNA-DNA hybridization. Virulence factors, resistance mechanisms, prophages, CRISPR elements, and metabolism elements were identified and annotated.</p><p><strong>Results: </strong>We confirmed the identity of the strains C960 and C2866 as Stenotrophomonas geniculata, and of strain C1657 as Stenotrophomonas indicatrix. The species designation parameters obtained indicated that the strains C4297 and C2852 are novel species. In comparison with the hypothetical proteome of the S. maltophilia complex species analyzed, elements associated with amino acid metabolism, DNA/RNA processing and repair, envelope biogenesis, and intracellular transport are predominant. Elements probably associated with antibiotic resistance, such as efflux pumps, aminoglycoside transferases, and phosphoethanolamine transferases, were identified, and the presence of genes related to capsule formation, iron acquisition, and intracellular survival probably contributes to virulence.</p><p><strong>Conclusions: </strong>This is the first report of S. geniculata and S. indicatrix as human pathogens. Besides, we proposed two novel species members of Smc: Stenotrophomonas veracruzanensis sp. nov. and Stenotrophomonas mexicanensis sp. nov.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143556901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing bacterial antimicrobial peptides: a comprehensive review on properties, mechanisms, applications, and challenges in combating antimicrobial resistance.","authors":"Pirasannah Erriah, Sheau Ling Puan, Normi Mohd Yahaya, Wan Nur Ismah Wan Ahmad Kamil, Syafinaz Amin Nordin, Azira Muhamad, Suriana Sabri","doi":"10.1093/jambio/lxae290","DOIUrl":"10.1093/jambio/lxae290","url":null,"abstract":"<p><p>Antimicrobial resistance (AMR) is a significant global health concern due to the persistence of pathogens and the emergence of resistance in bacterial infections. Bacterial-derived antimicrobial peptides (BAMPs) have emerged as a promising strategy to combat these challenges. Known for their diversity and multifaceted nature, BAMPs are notable bioactive agents that exhibit potent antimicrobial activities against various pathogens. This review explores the intricate properties and underlying mechanisms of BAMPs, emphasizing their diverse applications in addressing AMR. Additionally, the review investigates the mechanisms, analyses the challenges in utilizing BAMPs effectively, and examines their potential applications and associated deployment challenges providing comprehensive insights into how BAMPs can be harnessed to combat AMR across different domains. The significance of this review lies in highlighting the potential of BAMPs as transformative agents in combating AMR, offering sustainable and eco-friendly solutions to this pressing global health challenge.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143556926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}