Journal of Applied Microbiology最新文献_第5页

Membrane adaptation of Ochrobactrum intermedium under salt stress enables growth promotion in peanut plants. 盐胁迫下ochrobacum中间体的膜适应促进花生植株生长。

IF 3.2 3区生物学

Journal of Applied Microbiology Pub Date : 2025-09-01 DOI: 10.1093/jambio/lxaf227

Yanina B Reguera, Adriana B Cesari, Maria A Biasutti, Marta S Dardanelli, Natalia S Paulucci

{"title":"Membrane adaptation of Ochrobactrum intermedium under salt stress enables growth promotion in peanut plants.","authors":"Yanina B Reguera, Adriana B Cesari, Maria A Biasutti, Marta S Dardanelli, Natalia S Paulucci","doi":"10.1093/jambio/lxaf227","DOIUrl":"10.1093/jambio/lxaf227","url":null,"abstract":"Aims: Soil salinity significantly limits agricultural productivity in Argentina, posing a major threat to crops such as peanut (Arachis hypogaea). This study evaluated how the rhizospheric bacterium Ochrobactrum intermedium (L115) maintains its plant growth-promoting capacity under saline conditions through membrane-level adaptation mechanisms.Methods and results: Bacterial growth, cell morphology, membrane lipid composition, fluidity, and phase transition temperature (Tm) were analyzed under increasing NaCl concentrations (0, 0.4, 0.6, and 0.8 mol l-1). L115 tolerates up to 0.4-mol l-1 NaCl efficiently, preserving viability, morphology, and membrane biophysical properties similar to control conditions. This homeostasis was achieved by adjusting membrane phospholipids, notably increasing cardiolipin (29.4%) and phosphatidylcholine levels (3.2%), while maintaining a balanced zwitterionic/anionic phospholipid ratio. At 0.6 mol l-1, drastic changes in fatty acid and lipid profiles disrupted fluidity (0.175 to 0.131) and decreased Tm (12.5°C to 4°C), leading to reduced viability. At 0.8 mol l-1, severe changes in fluidity and Tm produced by increases in cardiolipin (88.2%) and phosphatidylcholine (21.4%) concentration and by an increase in unsaturated fatty acids led to cell dehydration, loss of flagella, and reduced viability. Peanut inoculation assays under 0.1-mol l-1 NaCl demonstrated that L115 significantly mitigated salt-induced growth reduction, restoring shoot and root biomass and length to levels comparable or superior to unstressed controls.Conclusions: These findings indicate that L115's ability to modulate its membrane composition allows it to sustain its function as a plant growth-promoting bacterium under moderate salt stress. Thus, L115 emerges as a promising bioinoculant candidate for enhancing crop resilience in saline soils.","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144992581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unleashing IbKTP-NH2, a kyotorphin derivative, against bacterial and fungal multispecies biofilm adhesion and viability on materials. 释放一种kyotorphin衍生物IbKTP-NH2，对细菌和真菌多物种生物膜在材料上的粘附和生存能力进行研究。

IF 3.2 3区生物学

Journal of Applied Microbiology Pub Date : 2025-09-01 DOI: 10.1093/jambio/lxaf205

Katia Conceição, Vitor M de Andrade, Vitor D M de Oliveira, Vasanthakumar G Ramu, Montserrat Heras, Eduard R Bardaji, Miguel A R B Castanho, Aline G Capella

{"title":"Unleashing IbKTP-NH2, a kyotorphin derivative, against bacterial and fungal multispecies biofilm adhesion and viability on materials.","authors":"Katia Conceição, Vitor M de Andrade, Vitor D M de Oliveira, Vasanthakumar G Ramu, Montserrat Heras, Eduard R Bardaji, Miguel A R B Castanho, Aline G Capella","doi":"10.1093/jambio/lxaf205","DOIUrl":"10.1093/jambio/lxaf205","url":null,"abstract":"Aims: Healthcare-associated infection (HAI) are a major global health concern, contributing to increased mortality and substantial economic burden on healthcare systems. This study aims to evaluate the efficacy of the peptide kyotorphin conjugated with ibuprofen (IbKTP-NH2) in inhibiting multispecies biofilms formed by Candida albicans, Pseudomonas aeruginosa, and Streptococcus pneumoniae, particularly in the context of biofilm-associated infections linked to implanted medical devices.Methods and results: Multispecies biofilms were cultured on polymeric and metallic materials. Antimicrobial susceptibility testing was performed to determine the minimum biofilm inhibitory concentrations (MBIC) of IbKTP-NH2 against the tested strains. Additionally, scanning electron microscopy (SEM) was utilized to analyze biofilm architecture, focusing on extracellular matrix production, cell density, and morphology. The study found that the MBIC for bacterial strains ranged from 46.5 to 1 mM. SEM analysis revealed significant biofilm disruption, characterized by reduced extracellular matrix production, decreased cell numbers, and altered cell morphology, indicating effective antimicrobial activity of IbKTP-NH2.Conclusion: The peptide IbKTP-NH2 demonstrates substantial inhibition of multispecies biofilms on both polymeric and metallic surfaces. These findings underscore its potential as an antimicrobial agent with possible antivirulence properties, and highlights IbKTP-NH2 as a promising candidate for the development of new therapeutic strategies aimed at preventing and controlling HAI and addressing chronic wound pathogens.","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144846597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Responses of microbial communities during oilseed plant-based phytoremediation of heavy metal-contaminated soils. 油籽植物修复重金属污染土壤中微生物群落的响应

IF 3.2 3区生物学

Journal of Applied Microbiology Pub Date : 2025-09-01 DOI: 10.1093/jambio/lxaf226

Guowei Zhang, Yihong Yue, Li Tu, Qunlu Liu, Qian Zhang, Kankan Shang

{"title":"Responses of microbial communities during oilseed plant-based phytoremediation of heavy metal-contaminated soils.","authors":"Guowei Zhang, Yihong Yue, Li Tu, Qunlu Liu, Qian Zhang, Kankan Shang","doi":"10.1093/jambio/lxaf226","DOIUrl":"10.1093/jambio/lxaf226","url":null,"abstract":"Aims: Phytoremediation is an effective method of remediating soils contaminated with heavy metals. However, it has some limitations in practical applications with regard to rare plant species, poor environmental adaptability, and long growth cycles. The dynamic response mechanisms of soil microbial communities during phytoremediation are still unclear, which restricts the optimization and promotion of this approach.Methods and results: No ethical approval was required for this study. In this study, soil bacterial, fungal, and archaeal communities during the remediation of Cu-, Pb-, and Zn-contaminated soils with five industrial oilseed plants [Xanthium strumarium (XS), Bidens pilosa (BP), Kosteletzkya virginica (KV), Sesbania cannabina (SC), and Commelina communis (CC)] were analyzed using metagenome sequencing. Compared with soil contaminated with heavy metals, remediation through five industrial oilseed plants significantly reduced the content of heavy metals in the soil, with soil Cu, Pb, and Zn decreasing by 44.01%, 46.32%, and 27.62%, respectively, and WSCu, WSPb, and WSZn content decreasing by 28.23%, 50.68%, and 75.26%, respectively. Microbial diversity analysis showed that the phytoremediation significantly affected the soil microbial communities, with a significant decrease in archaeal diversity. Variation partitioning analysis and Mantel tests revealed that heavy metals and soil physicochemical properties significantly affected microbial communities, and heavy metals exerted stronger effects on archaeal communities. Meanwhile, soil contaminated with heavy metals was mainly dominated by fungal-fungal interactions, whereas phytoremediation increased the complexity of microbial symbiotic networks.Conclusion: Collectively, these results provide fundamental insights into the microbial community structure during phytoremediation of heavy metal-contaminated soil, which may aid in the bioregulation of phytoremediation.","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145000659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gut-organ axis is more fragmented in ulcerative colitis: a metaproteomics study. 一项宏蛋白质组学研究表明，溃疡性结肠炎患者的内脏器官轴更碎片化。

IF 3.2 3区生物学

Journal of Applied Microbiology Pub Date : 2025-09-01 DOI: 10.1093/jambio/lxaf207

Asha Yadav, Pratik Balwant Shinde, Arush Behl, Deepti Singh, Saurabh Kedia, Vineet Ahuja, Krishna Kant Sharma

{"title":"Gut-organ axis is more fragmented in ulcerative colitis: a metaproteomics study.","authors":"Asha Yadav, Pratik Balwant Shinde, Arush Behl, Deepti Singh, Saurabh Kedia, Vineet Ahuja, Krishna Kant Sharma","doi":"10.1093/jambio/lxaf207","DOIUrl":"10.1093/jambio/lxaf207","url":null,"abstract":"Aims: The study aims to assess the gut microbiota function and the status of bidirectional relationship of gut with other body organs in ulcerative colitis (UC).Methods and results: The proteomic analysis of fecal samples revealed the presence of 2072 microbial proteins and 243 human proteins. Microbial proteins for pentose phosphate pathway (PPP) increased significantly in UC samples; whereas, decrease in proteins related to energy metabolism, transmembrane transporters and membrane proteins, and protein folding was noted. Interestingly, expression of proteins related to iron metabolism and oxidative stress indicates an oxidative gut environment that favors the growth of pathogenic microbes, enteric infections, and intestinal damage. Over-expression of host proteins such as neutrophil defensin 3, lactotransferrin, neutrophil elastase, azurocidin, protein S100-A8, protein S100-A9, and cathepsin G further indicate inflammation, and microbial infection in UC gut. The microbial (Omp)-host (TLR4) protein interaction analysis by molecular docking suggests significant implications in immune modulation. Further, gas chromatography-mass spectrometry investigation revealed altered concentration of microbial short chain fatty acids.Conclusion: The study revealed vital clues about the gut-organ homeostasis in UC that contribute to the host physiology, disease pathogenesis and 'gut-organ axis' fragmentation.","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144873330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extended persistence and thermostability of adenovirus 41 in the presence of bacteria. 腺病毒41在细菌存在下的持久性和热稳定性。

IF 3.2 3区生物学

Journal of Applied Microbiology Pub Date : 2025-09-01 DOI: 10.1093/jambio/lxaf221

Justin C Greaves, Balghsim Alshehri, Olivia N Birch, Roberto A Rodriguez, Christopher M Robinson, Daniel B Kearns, Sharon C Kosgei

{"title":"Extended persistence and thermostability of adenovirus 41 in the presence of bacteria.","authors":"Justin C Greaves, Balghsim Alshehri, Olivia N Birch, Roberto A Rodriguez, Christopher M Robinson, Daniel B Kearns, Sharon C Kosgei","doi":"10.1093/jambio/lxaf221","DOIUrl":"10.1093/jambio/lxaf221","url":null,"abstract":"Aims: This study aimed to examine the persistence of adenovirus 41 (AdV41) in the presence of a specific bacteria, namely Bacillus subtilis. Additionally, our study aimed to determine heat inactivation of AdV41 in the presence of bacteria.Methods and results: Cultivable methods were utilized to measure the decay of AdV41 in different conditions, including the presence/absence of intestinal bacteria, B. subtilis, and various strains of B. subtilis. In the current study, we first examined the interaction between an intestinal bacterial population and AdV41. We determined that the bacteria were able to extend AdV41 stability at 37°C. As extracellular polymeric substances (EPS) are essential to biofilm formation and environmental bacterial persistence, we then tested the enhancing abilities of two different strains of the bacteria B. subtilis, an EPS overproducing strain (EPS+) and an EPS nonproducing strain (EPS-). We determined that the EPS+ strain was able to extend AdV41 persistence and infection at higher temperatures (45 and 56°C).Conclusions: Overall, this data suggests that specific strains of bacteria can further extend the persistence of AdV41 above other strains. This is also the first study to highlight the importance of EPS in enteric viral stability.","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144955342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Revitalizing health with Lactobacillus isolates: mitigating aflatoxin B1 toxicity in HT-29 cell line and Balb/c mice. 用分离乳杆菌恢复健康：减轻HT-29细胞系和Balb/c小鼠黄曲霉毒素B1毒性。

IF 3.2 3区生物学

Journal of Applied Microbiology Pub Date : 2025-09-01 DOI: 10.1093/jambio/lxaf210

Atieh Darbandi, Shabnam Zeighamy Alamdary, Soheila Shahroodian, Behrooz Sadeghi Kalani, Esmaeil Samizadeh, Mahsa Ghamari, Naser Kakavandi, Malihe Talebi

{"title":"Revitalizing health with Lactobacillus isolates: mitigating aflatoxin B1 toxicity in HT-29 cell line and Balb/c mice.","authors":"Atieh Darbandi, Shabnam Zeighamy Alamdary, Soheila Shahroodian, Behrooz Sadeghi Kalani, Esmaeil Samizadeh, Mahsa Ghamari, Naser Kakavandi, Malihe Talebi","doi":"10.1093/jambio/lxaf210","DOIUrl":"10.1093/jambio/lxaf210","url":null,"abstract":"Aim: Aflatoxins (AFs), carcinogenic mycotoxins found in fermented foods, animal gastrointestinal tracts (GITs), and the environment, can be detoxified by probiotic lactobacilli. The aim of this study was to test whether probiotic lactobacilli can detoxify AFs, carcinogenic mycotoxins prevalent in fermented foods, animal GITs, and the environment.Methods and results: Five candidate Lactobacillus strains [Lacticaseibacillus rhamnosus 195 (G1), Lactiplantibacillus plantarum 42 (G2), Levilactobacillus brevis 205 (G3), L. plantarum 165 (G4), and Limosilactobacillus reuteri 100 (G5)] were prepared to compare their ability to reduce aflatoxicosis (AFB1) in vitro and in vivo by evaluating apoptotic factors, inflammatory markers, tight junction, and nutrient transport genes. G1, G2, G3, G4, and G5 bound AFB1 well (with no notable variations). These probiotic isolates lowered Bax and caspase-3 expression in AFB1-treated HT-29 cells and raised Bcl-2 expression. In AFB1-treated cells, these probiotics increased occludin and zonula occludens-1 expression as tight junction markers. In contrast, Lactobacillus strains + AFB1-treated cells expressed fewer nutritional transport genes (amino acid transporter 2, glucose transporter 2, peptide transporter 1, sodium-dependent glucose cotransporter 1) and interleukin-6, an inflammatory marker. In animal models, oral Lactobacillus supplementation reduced AFB1-induced liver injury and increased GST A3 expression via the Nrf2 pathway. These Lactobacillus isolates also reduced CYP 450 1A2 and 3A4 expression.Conclusions: The results of this study suggest that Lactobacillus strains could be used as a protective strategy against mycotoxin cytotoxicity by promoting the maintenance of standard intestinal cell structure and function and the health of animal models.","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144955376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Successful establishment of a model of the piglet gastrointestinal microbiota and its' modulation by prebiotics. 成功建立仔猪胃肠道微生物群模型及益生元对其调节作用。

IF 3.2 3区生物学

Journal of Applied Microbiology Pub Date : 2025-09-01 DOI: 10.1093/jambio/lxaf216

Bruno P Milnitsky, Harry L P Junior, Beni J M Chaúque, Gertrudes Corção

{"title":"Successful establishment of a model of the piglet gastrointestinal microbiota and its' modulation by prebiotics.","authors":"Bruno P Milnitsky, Harry L P Junior, Beni J M Chaúque, Gertrudes Corção","doi":"10.1093/jambio/lxaf216","DOIUrl":"10.1093/jambio/lxaf216","url":null,"abstract":"Aims: This study proposed an in-house in vitro model to investigate the effects of two prebiotic treatments on the gastrointestinal microbiota of piglets.Methods and results: The model involved suspending piglet feces in a culture medium to simulate the ileum and proximal colon regions of the swine gastrointestinal tract. The prebiotics tested were mannanoligosaccharides (MOS) and sodium butyrate. Metabarcoding and culturomics were used to assess the impact of prebiotics on bacterial species composition. Minimum inhibitory concentration tests were conducted to examine bacterial susceptibility patterns. Key bacterial phyla identified included firmicutes, proteobacteria, bacteroidetes, and actinobacteria. Culturomics detected families and several species not identified by metabarcoding. In the simulated proximal colon, MOS increased the abundance of certain species and reduced bacteria with type I fimbriae. Butyric acid promoted beneficial host-associated bacteria and decreased pathogenic species. However, the prebiotics did not significantly affect bacterial susceptibility to antibiotics.Conclusion: The in-house model successfully mimicked piglet intestinal microbiota, allowing for detailed analysis. Both prebiotics positively influenced the piglets microbiota, providing insights into how these treatments potentially influenced the microbiota.","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144955170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Two thermostable xylanases with different acid-alkalinity coexistence in one bacterium screened using lignocellulosic biomass and its applications. 利用木质纤维素生物质筛选一种细菌中不同酸碱共存的两种耐热木聚糖酶及其应用。

IF 3.2 3区生物学

Journal of Applied Microbiology Pub Date : 2025-09-01 DOI: 10.1093/jambio/lxaf212

Caixia Cui, Shuanglong Wei, Fubo Niu, Yanghan Peng, Hong Ming

{"title":"Two thermostable xylanases with different acid-alkalinity coexistence in one bacterium screened using lignocellulosic biomass and its applications.","authors":"Caixia Cui, Shuanglong Wei, Fubo Niu, Yanghan Peng, Hong Ming","doi":"10.1093/jambio/lxaf212","DOIUrl":"10.1093/jambio/lxaf212","url":null,"abstract":"Aims: This study aimed to isolate and characterize thermostable xylanases from the thermophilic bacterium Geobacillus stearothermophilus CFH 71344, screened from Yunnan hot springs, for their potential in lignocellulosic biomass conversion and xylooligosaccharide (XOS) production.Methods and results: Two xylanases, Xyn2415 and Xyn2429, were heterologously expressed and biochemically characterized. Biochemical characterization revealed that enzyme Xyn2415 exhibited optimal activity at pH 9.0 and 70°C, while enzyme Xyn2429 showed optimal activity at pH 6.0 and 80°C. Both enzymes demonstrated significant thermostability, retaining considerable activity even after prolonged exposure to high temperatures. The kinetic parameters indicated that Xyn2429 had higher catalytic efficiency than Xyn2415. The enzymes also exhibited distinct substrate preferences and metal ion sensitivities. When applied to beechwood xylan hydrolysis, Xyn2415 and Xyn2429 produced different XOS profiles, with Xyn2415 preferentially generating short-chain XOS and Xyn2429 producing longer-chain XOS.Conclusions: The coexistence of these enzymes in one bacterium highlights their adaptability to varying environmental conditions, offering potential for cost-effective and efficient industrial applications in lignocellulosic biorefining and prebiotic production.","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144955089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intra-tumoral microbial heterogeneity of breast cancer: roles in tumorigenesis, therapeutic responses, and future directions. 乳腺癌的肿瘤内微生物异质性：在肿瘤发生、治疗反应和未来方向中的作用。

IF 3.2 3区生物学

Journal of Applied Microbiology Pub Date : 2025-09-01 DOI: 10.1093/jambio/lxaf230

Lili Jiang, Man Li, Zuowei Zhao

{"title":"Intra-tumoral microbial heterogeneity of breast cancer: roles in tumorigenesis, therapeutic responses, and future directions.","authors":"Lili Jiang, Man Li, Zuowei Zhao","doi":"10.1093/jambio/lxaf230","DOIUrl":"https://doi.org/10.1093/jambio/lxaf230","url":null,"abstract":"Recent advances in microbiome research have revealed that tumor-resident microbiota are not passive bystanders but active contributors to the progression of breast cancer. Similar to the well-characterized gut-breast microbiota axis, emerging evidence points to intricate interactions between intra-tumoral microbiota and breast cancer with implications for carcinogenesis, therapeutic response, and future directions. Intra-tumoral microbes have been shown to initiate inflammation, modulate tumor microenvironment, alter drug metabolism, and produce bioactive metabolites that influence tumor cell proliferation, apoptosis, and epithelial-mesenchymal transition. Distinct microbial signatures have been associated with specific molecular subtypes of breast cancer and may serve as predictive biomarkers for prognosis. Furthermore, dysbiosis within the tumor-resident microbiota has been linked to the development of treatment resistance, including chemotherapy, immunotherapy, and endocrine therapy. Preclinical studies support the feasibility of modulating the microbiota via using antibiotics, probiotics, fecal microbiota transplantation, or bacteriophage-based strategies to enhance antitumor efficacy and overcome resistance. This review summarizes current knowledge on the biological roles of tumor-resident microbiota in breast cancer, highlights subtype-specific microbial patterns and host-microbe interactions, and explores microbiota-targeted interventions as promising adjuncts in overcoming drug resistance.","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":"136 9","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation of bacteria and yeast from blood using antibody-conjugated magnetic beads. 抗体偶联磁珠从血液中分离细菌和酵母菌。

IF 3.2 3区生物学

Journal of Applied Microbiology Pub Date : 2025-09-01 DOI: 10.1093/jambio/lxaf215

Manuel García-Algar, Anna Mur, Marina B Velasco, Sergi Casellas, Ander Eguskiza, Albert Ferriol, Sara Gómez-de Pedro

{"title":"Isolation of bacteria and yeast from blood using antibody-conjugated magnetic beads.","authors":"Manuel García-Algar, Anna Mur, Marina B Velasco, Sergi Casellas, Ander Eguskiza, Albert Ferriol, Sara Gómez-de Pedro","doi":"10.1093/jambio/lxaf215","DOIUrl":"10.1093/jambio/lxaf215","url":null,"abstract":"Aims: Rapid detection of bloodstream infection pathogens is essential, as traditional blood cultures require 24-72 h, delaying treatment and reducing survival rates. This study aims to develop a simple, efficient strategy for rapidly isolating Pseudomonas aeruginosa (Gram negative), Staphylococcus aureus (Gram positive), and Candida albicans (yeast) directly from blood while preserving whole-cell integrity for downstream applications such as fluorescence staining.Methods and results: The method employs antibody-conjugated magnetic beads (MBs) to capture pathogens directly from blood, enhancing detection sensitivity for low-abundance organisms. Pathogens are eluted as intact cells, enabling visualization via fluorescence staining. The approach successfully isolated P. aeruginosa, S. aureus, and C. albicans, demonstrating robust performance and clinical potential.Conclusions: Antibody-conjugated magnetic beads offer a rapid, sensitive, and efficient solution for pathogen isolation from blood, maintaining cell integrity for further analysis. This method improves detection and diagnosis of BSIs, supporting timely interventions and better patient outcomes.","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144955295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0