M. Pérez-Bernal, Carlos Hernández, Magalis Delgado, Z. Cinza, M. Izquierdo, Nelvis Figueroa, P. Díaz, Enrique Pérez
{"title":"Full validation of an ELISA for quantification of antibodies against Haemophilus influenzaetype b capsular polysaccharide","authors":"M. Pérez-Bernal, Carlos Hernández, Magalis Delgado, Z. Cinza, M. Izquierdo, Nelvis Figueroa, P. Díaz, Enrique Pérez","doi":"10.15406/jabb.2021.08.00247","DOIUrl":"https://doi.org/10.15406/jabb.2021.08.00247","url":null,"abstract":"Since 1981, ELISA methods have been used for the quantification of antibodies against Haemophilus influenzae type b (Hib), but their complete validation has been never reported. This work described the full validation of an ELISA for the quantification of antibodies against Hib capsular polysaccharide, following eleven parameters recommended by European Medicines Agency and FDA guidelines. The method satisfied the acceptance criteria for the required parameters. The use of percent of inhibition and parallelism fulfillment as acceptance criteria for specificity was discussed. This ELISA will be a reliable device for the evaluation of Hib-vaccination efficacious.d","PeriodicalId":15033,"journal":{"name":"Journal of Applied Biotechnology & Bioengineering","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89042771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Protective effects of ethanolic extract of Alhagi maurorum roots on renal failure induced by acetaminophen in mice","authors":"Seham I AL-Nafea, M. O. Aljahdali","doi":"10.15406/JABB.2021.08.00246","DOIUrl":"https://doi.org/10.15406/JABB.2021.08.00246","url":null,"abstract":"The protective actions of ethanol Alhagi maurorum (AM) root ethanol extract on acetaminophen-induced oxidative stress and renal toxicity in mice was evaluated. Forty male SWR strain albino mice aged 8 weeks were grouped into five groups. G1 (n=5): as control. G2 (n=5): administered orally a single dose of acetaminophen (2000mg/kg). G3 (n=10) administrated orally 200 mg/kg of roots ethanol extract for one week then acetaminophen as G2 at 8th day and; G4 (n=10) administrated orally 400 mg/kg of roots ethanol extract for one week then acetaminophen as G2 at 8th day; G5 (n=10) administrated orally 600 mg/kg of roots ethanol extract for one week then acetaminophen as G2 at 8th day. At end of experiments, the mice were killed under anesthesia and blood samples were gathered to preform complete blood test (CBC), serum levels of urea and creatinine and oxidative stress biomarkers as superoxide dismutase (SOD), glutathione (GSH), and catalase (CAT) using available Elisa mice kits. Kidneys were removed and histologically examined. Acetaminophen intake significantly elevated WBCs, neutrophils, monocytes, urea and creatinine levels and significantly decreased RBCs, hemoglobin, hematocrit, GSH, SOD and CAT (P <0.05). Treatment with Alhagi maurorum roots extract especially high dose (600 mg/kg) resulted in decreased in WBCs, neutrophils, monocytes, urea and creatinine levels and significantly increased RBCs, hemoglobin, hematocrit, GSH, SOD and CATversusacetaminophen group. Alhagi maurorum root extract treatment similarly decreased renal histological alteration induced by acetaminophen. This study can be utilized as prove of reading that Alhagi maurorum ethanol root extract especially high dose might be administered to prevent renal destruction induced by acetaminophen due to its antioxidant activity","PeriodicalId":15033,"journal":{"name":"Journal of Applied Biotechnology & Bioengineering","volume":"83 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83807432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Neuromodulation: a review of deep brain stimulation devices for the treatment of Parkinson’s disease","authors":"N. Noli, M. Jackson, Tawil Bill","doi":"10.15406/JABB.2021.08.00245","DOIUrl":"https://doi.org/10.15406/JABB.2021.08.00245","url":null,"abstract":"opioids.2 A Spinal Cord Stimulator (SCS) is a neuromodulation device that can be used to treat chronic pain.3 SCS is a tool that stimulates the basal ganglion with adjustable bursts and high frequency stimulation that can target specific sources of pain. The implantable leads are adjustable and allow the device to zero in on the specific synapse that is causing pain; this disrupts the messaging of that synapse with electrical impulses.2","PeriodicalId":15033,"journal":{"name":"Journal of Applied Biotechnology & Bioengineering","volume":"82 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75814710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Moubasher, Salwa S. Wahsh, Nabil Abo El-Kassem, R. Ali
{"title":"Aureobasidium pullulans Gene from Egypt","authors":"H. Moubasher, Salwa S. Wahsh, Nabil Abo El-Kassem, R. Ali","doi":"10.15406/JABB.2021.08.00244","DOIUrl":"https://doi.org/10.15406/JABB.2021.08.00244","url":null,"abstract":"Sequancing of pullulanase from the fungus Aureobasidium pullulans isolated from Egypt soil; Genomic DNA of pullulanase was determined for the first time using PCR, according to Baser program, Pullulanase nucleotide collection from Aureobasidium pullulans was blasted which showed similarity using NCBI significant alignment with Aureobasidium namibiae CBS 147.97 hypothetical protein partial mRNA and 46 % with Aureobasidium pullulans JQ624241 and AF470619; Identified sequenced fragment was 2051 bp. and G+C content is 50.5% with molecular mass 63 KDa.","PeriodicalId":15033,"journal":{"name":"Journal of Applied Biotechnology & Bioengineering","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74020217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sana Shahzadi, Isha Ishtiaq, Khadija Aslam, U. Ali, Savera Mehak, Sara Khan, Shanza Sajjad, Maria Babar
{"title":"3D bioprinting–a step towards heart tissue regeneration","authors":"Sana Shahzadi, Isha Ishtiaq, Khadija Aslam, U. Ali, Savera Mehak, Sara Khan, Shanza Sajjad, Maria Babar","doi":"10.15406/JABB.2021.08.00243","DOIUrl":"https://doi.org/10.15406/JABB.2021.08.00243","url":null,"abstract":"Heart disease and cardiovascular disease is a very serious and growing public health issue. Tissue-engineering has great potential and great strength for regeneration, remolding, and growth. In the case of heart failure, Allografting has been used. 3D bioprinting has a great impact in the field of cardiovascular tissue engineering. It has been observed that 3D Bioprinting is used to construct an artificial heart for transplantation and used to create myocardial cells in case of injury. Recent studies showed that biomaterial used in the treatment of myocardial dysfunction is decellularized cardiac extracellular matrix hydrogel in adults. Collagen, Alginate gelatin, hyaluronic acid, and deECM scaffolds were used as biomaterials in 3D bioprinting. It has been shown that scaffold used with ECM was used to support there generation process A new 3D bioprinting technology was developed in which cells were collected into spheroids and printed on a needle array according to desirable characteristics. Different bio inks such as laser, extrusion, droplet, and stereolithography are used here. Electric stimulation is key to the contractility of cardiomyocytes. A physical cardiac replica was created by image processing software that creates 3D structures. In holographic display 3D, full hearts of patients were printed in flexible material. A process is demonstrated to fabricate robust valves of the heart using the3D bioprinting technique. MRI or CT scans were used to obtained 3D images of the aorta.3D bioprinting plays a huge role in knowing the aortic anatomy involves the aortic valve area and morphology of the root. Recent advances demonstrated that 3D bioprinting can assist in ventricular device placement and perform a specific function in a complex with (CHD) Congenital heart defects. 3D bioprinting holds great prom","PeriodicalId":15033,"journal":{"name":"Journal of Applied Biotechnology & Bioengineering","volume":"245 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80572706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Fruit orchards under climate change conditions: adaptation strategies and management","authors":"W. Abobatta","doi":"10.15406/jabb.2021.08.00260","DOIUrl":"https://doi.org/10.15406/jabb.2021.08.00260","url":null,"abstract":"Under global warming and climate change conditions fruit orchards facing different environmental challenges which cause negative impacts on the growth and productivity of various fruit trees particularly in arid and semi-arid areas, various abiotic stress such as rising temperature, drought, heatwaves, and soil salinity represented a major challenge for growth and productivity of fruit orchards. Fruit trees used different strategies to cope with abiotic stress and minimize their adverse effects. Plants used different physiological, anatomical, and morphological mechanisms to tolerate abiotic stress, such as ion homeostasis, synthesis of more compatible solute, polyamines production, antioxidant regulation, closing stomata, in addition tol modification of root system, abscission of the leaves partially, compactness canopy, reducing leaf size, furthermore, under abiotic stress plants produce various organic solutes to cope with Reactive Oxygen solutes like Proline, in addition, using proper management practices that include providing adequate nutrients requirement particularly Potassium and Calcium, maintain soil moisture, using proper rootstocks tolerant for drought and salinity stress as well as exogenous application of plant growth substances could sustain orchards growth and productivity","PeriodicalId":15033,"journal":{"name":"Journal of Applied Biotechnology & Bioengineering","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88648052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bethsua Mendoza, Edna Maria Hernandez, Leyri Desireth Romo, Apolonio Vargas, Jorge Alvez Cervantes, A. Fernández
{"title":"Edible films based on chayotextle starch and its effect on the shelf life of apples","authors":"Bethsua Mendoza, Edna Maria Hernandez, Leyri Desireth Romo, Apolonio Vargas, Jorge Alvez Cervantes, A. Fernández","doi":"10.15406/jabb.2021.08.00249","DOIUrl":"https://doi.org/10.15406/jabb.2021.08.00249","url":null,"abstract":"The apple is a climacteric fruit, characterized by having a long post-harvest life in optimal storage conditions, in addition to various nutritional benefits. In Mexico, it has become common to sell apple by piece and ready to be consumed, each piece after being washed and disinfected, is protected with a polyurethane net and a low-density polyethylene plastic film, which contributes to the increase in the generation of solid waste and environmental pollution. The main objective of this work was to evaluate the effectiveness of edible films based on chayotextle starch and pectin with nopal mucilage, to preserve the physicochemical, microbiological and sensory characteristics of apples stored in refrigeration (4-6°C). Two types of films were made: T1: chayotextle starch (4%), glycerol (2%); T2: pectin (1%), nopal mucilage (0.5%), glycerol (30% w / v); These were determined the permeability to water vapor (T1: 1.317x10-12; T2: 8.854 g s- 1 Pa-1 m -1) and oxygen permeability (T1: 4.444x10-14, T2: 5.87 gm s -1 Pa-1 m-2). The shelf-life study in apples showed that both the edible films and the plastic material maintained the physicochemical (pH), sensory, rheological (Hardness) and microbiological (fungi and yeast, mesophilic aerobic and total coliform) characteristics of the apples for 40 days","PeriodicalId":15033,"journal":{"name":"Journal of Applied Biotechnology & Bioengineering","volume":"72 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77285833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Fungi consortia in situ biodegradation of xenobiotic, military shooting range, Kachia, Kaduna, Nigeria","authors":"Ayodele A. Otaiku, A. Alhaji","doi":"10.15406/JABB.2020.07.00241","DOIUrl":"https://doi.org/10.15406/JABB.2020.07.00241","url":null,"abstract":"A major limitation of the white-rot fungus is its sensitivity during biodegradation of mixed matrix explosive pollutants and the scale of Kachia military shooting since 1967, Nigeria. The amplified 16S rRNA gene of each microbial isolate was processed for sequencing and characterization with Gene Bank database. Fungal species heavy metal reduction in increasing order of Aspergillus niger > Trametes versicolor > Rhizopus spp > Phanorochate chrysoporium > Penicillium spp were identified. The total explosive contents shows a significant difference for all locations in both dry and wet seasons (P<0.05) using Anova test. Microbial fungi consortium (MFC) bioremediate heavy metal significantly at 61.7% relative to isolated fungi species because of the lateral gene transfer/co-metabolism, where Trametes versicolor and Aspergillus niger act as gene mediators. MFC growth in 1% mineral salt medium munitions was significance than fungal species isolate. Deploying Myco Bio-augmentation / Phytoremediation/Biosimulation (Myco B-P-B) techniques to optimize the RDX and HMX characterized by a higher Nitrogen/Carbon ratio since fungi lack the beta-glucuronidase (GUS) gene to utilize carbon source directly. Pollutants bio-stimulation will enhances co-metabolism by MFC. Plant detoxification capabilities can be improved using fungi genes laccases and cytochrome P450 monooxygenase expressed effectively in plants using protoplast fusion","PeriodicalId":15033,"journal":{"name":"Journal of Applied Biotechnology & Bioengineering","volume":"55 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72844115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Microbes as remediating agents in detoxification of dyes","authors":"P. Chaurasia, S. L. Bharati, Sunil Kumar","doi":"10.15406/JABB.2020.07.00240","DOIUrl":"https://doi.org/10.15406/JABB.2020.07.00240","url":null,"abstract":"Dyes are colored substances being used to color various types of fabrics and materials from ancient time. During their use in the coloring of various types of materials as well as during their synthesis; they come out in the environment as such or in the form of toxic by-products and by mixing in aqueous system generate various types of problems to organisms living in that aqueous environment as well as human beings using that polluted water in any way. Several techniques are being used in the present time to solve such environmental problems. In this direction, many researches are also running on the green use of microbial technology in bioremediation of toxic, carcinogenic and extremely harmful unused dye’s products as well as its by-products by elimination or degradation or de-colorization. This mini-review discusses about the some recent researches done in this field in order to solve this problem and future aspect of the use of this green technology","PeriodicalId":15033,"journal":{"name":"Journal of Applied Biotechnology & Bioengineering","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88476576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Identification and in situ hybridization to mitotic chromosomes of a molecular marker linked to maleness in Anastrepha fraterculus (Wied.)","authors":"A. Basso, A. Sonvico","doi":"10.15406/jabb.2020.07.00239","DOIUrl":"https://doi.org/10.15406/jabb.2020.07.00239","url":null,"abstract":"The present report shows the molecular identification, isolation and citologically localization of a DNA-sequence from the South American fruit fly Anastrepha fraterculus (DIPTERA: Tephritidae) involved in sex- determination. It belongs to the Tephritidae family, the true fruit flies which are consider a pest of fruit crops. The sex determination system is of vital importance in the genetic control of the fruit fly pest: Sterile Insect Technique which unlike chemical control tactics, is environmentally friendly and does not pose any health concerns. We used in situ hybridization on mitotic chromosomes for localizing the primary sex determination factor in this fruit fly pest. Our results show that in Anastrepha fraterculus the Y chromosome is responsible for sex determination","PeriodicalId":15033,"journal":{"name":"Journal of Applied Biotechnology & Bioengineering","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85142861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}