H. Moubasher, Salwa S. Wahsh, Nabil Abo El-Kassem, R. Ali
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引用次数: 0
摘要
埃及土壤中普鲁兰小孢子菌普鲁兰酶的序列测定根据Baser程序，首次采用PCR方法对pululanase基因组DNA进行了测定，对pululanase收集的pululanase核苷酸进行了分析，结果表明，该序列与纳米Aureobasidium namibiae CBS 147.97假设蛋白部分mRNA具有显著的同源性，与pululans JQ624241和AF470619的同源性为46%;鉴定出的序列片段长度为2051 bp。G+C含量为50.5%，分子量为63 KDa。
Sequancing of pullulanase from the fungus Aureobasidium pullulans isolated from Egypt soil; Genomic DNA of pullulanase was determined for the first time using PCR, according to Baser program, Pullulanase nucleotide collection from Aureobasidium pullulans was blasted which showed similarity using NCBI significant alignment with Aureobasidium namibiae CBS 147.97 hypothetical protein partial mRNA and 46 % with Aureobasidium pullulans JQ624241 and AF470619; Identified sequenced fragment was 2051 bp. and G+C content is 50.5% with molecular mass 63 KDa.
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