毛竹根乙醇提取物对对乙酰氨基酚致小鼠肾功能衰竭的保护作用

Seham I AL-Nafea, M. O. Aljahdali
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引用次数: 1

摘要

研究了毛竹根乙醇提取物对对乙酰氨基酚诱导的小鼠氧化应激和肾毒性的保护作用。选取8周龄雄性SWR系白化小鼠40只,随机分为5组。G1 (n=5):作为对照。G2 (n=5):口服单剂量对乙酰氨基酚(2000mg/kg)。G3组(n=10)口服根乙醇提取物200 mg/kg 1周,第8天给予对乙酰氨基酚作为G2;G4组(n=10)给予根乙醇提取物400 mg/kg口服1周,第8天给予对乙酰氨基酚作为G2;G5组(n=10)给予根乙醇提取物600 mg/kg口服1周,第8天给予对乙酰氨基酚作为G2。实验结束时,在麻醉下处死小鼠,采集血样进行全血检测(CBC)、血清尿素和肌酐水平以及氧化应激生物标志物超氧化物歧化酶(SOD)、谷胱甘肽(GSH)和过氧化氢酶(CAT)。取肾行组织学检查。对乙酰氨基酚显著提高了白细胞、中性粒细胞、单核细胞、尿素和肌酐水平,显著降低了红细胞、血红蛋白、红细胞压积、GSH、SOD和CAT水平(P <0.05)。特别是高剂量(600 mg/kg)的毛蒿根提取物显著降低了白细胞、中性粒细胞、单核细胞、尿素和肌酐水平,显著升高了红细胞、血红蛋白、红细胞压积、GSH、SOD和CATversusacetaminophen组。对乙酰氨基酚引起的肾组织改变,毛竹根提取物处理也有相似的降低作用。本研究可以作为对乙酰氨基酚抗氧化作用的证据,特别是大剂量的毛竹乙醇根提取物可以预防对乙酰氨基酚引起的肾损害
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Protective effects of ethanolic extract of Alhagi maurorum roots on renal failure induced by acetaminophen in mice
The protective actions of ethanol Alhagi maurorum (AM) root ethanol extract on acetaminophen-induced oxidative stress and renal toxicity in mice was evaluated. Forty male SWR strain albino mice aged 8 weeks were grouped into five groups. G1 (n=5): as control. G2 (n=5): administered orally a single dose of acetaminophen (2000mg/kg). G3 (n=10) administrated orally 200 mg/kg of roots ethanol extract for one week then acetaminophen as G2 at 8th day and; G4 (n=10) administrated orally 400 mg/kg of roots ethanol extract for one week then acetaminophen as G2 at 8th day; G5 (n=10) administrated orally 600 mg/kg of roots ethanol extract for one week then acetaminophen as G2 at 8th day. At end of experiments, the mice were killed under anesthesia and blood samples were gathered to preform complete blood test (CBC), serum levels of urea and creatinine and oxidative stress biomarkers as superoxide dismutase (SOD), glutathione (GSH), and catalase (CAT) using available Elisa mice kits. Kidneys were removed and histologically examined. Acetaminophen intake significantly elevated WBCs, neutrophils, monocytes, urea and creatinine levels and significantly decreased RBCs, hemoglobin, hematocrit, GSH, SOD and CAT (P <0.05). Treatment with Alhagi maurorum roots extract especially high dose (600 mg/kg) resulted in decreased in WBCs, neutrophils, monocytes, urea and creatinine levels and significantly increased RBCs, hemoglobin, hematocrit, GSH, SOD and CATversusacetaminophen group. Alhagi maurorum root extract treatment similarly decreased renal histological alteration induced by acetaminophen. This study can be utilized as prove of reading that Alhagi maurorum ethanol root extract especially high dose might be administered to prevent renal destruction induced by acetaminophen due to its antioxidant activity
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