Aureobasidium pullulans Gene from Egypt

H. Moubasher, Salwa S. Wahsh, Nabil Abo El-Kassem, R. Ali
{"title":"Aureobasidium pullulans Gene from Egypt","authors":"H. Moubasher, Salwa S. Wahsh, Nabil Abo El-Kassem, R. Ali","doi":"10.15406/JABB.2021.08.00244","DOIUrl":null,"url":null,"abstract":"Sequancing of pullulanase from the fungus Aureobasidium pullulans isolated from Egypt soil; Genomic DNA of pullulanase was determined for the first time using PCR, according to Baser program, Pullulanase nucleotide collection from Aureobasidium pullulans was blasted which showed similarity using NCBI significant alignment with Aureobasidium namibiae CBS 147.97 hypothetical protein partial mRNA and 46 % with Aureobasidium pullulans JQ624241 and AF470619; Identified sequenced fragment was 2051 bp. and G+C content is 50.5% with molecular mass 63 KDa.","PeriodicalId":15033,"journal":{"name":"Journal of Applied Biotechnology & Bioengineering","volume":"50 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2021-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Applied Biotechnology & Bioengineering","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.15406/JABB.2021.08.00244","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Sequancing of pullulanase from the fungus Aureobasidium pullulans isolated from Egypt soil; Genomic DNA of pullulanase was determined for the first time using PCR, according to Baser program, Pullulanase nucleotide collection from Aureobasidium pullulans was blasted which showed similarity using NCBI significant alignment with Aureobasidium namibiae CBS 147.97 hypothetical protein partial mRNA and 46 % with Aureobasidium pullulans JQ624241 and AF470619; Identified sequenced fragment was 2051 bp. and G+C content is 50.5% with molecular mass 63 KDa.
来自埃及的普鲁兰小孢子虫基因
埃及土壤中普鲁兰小孢子菌普鲁兰酶的序列测定根据Baser程序,首次采用PCR方法对pululanase基因组DNA进行了测定,对pululanase收集的pululanase核苷酸进行了分析,结果表明,该序列与纳米Aureobasidium namibiae CBS 147.97假设蛋白部分mRNA具有显著的同源性,与pululans JQ624241和AF470619的同源性为46%;鉴定出的序列片段长度为2051 bp。G+C含量为50.5%,分子量为63 KDa。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信