Journal of Applied Biotechnology & Bioengineering最新文献_第4页

Facing COVID 19 pandemic: experience from a clinical microbiology laboratory in Spain 面对COVID - 19大流行:来自西班牙临床微生物实验室的经验

Journal of Applied Biotechnology & Bioengineering Pub Date : 2020-11-02 DOI: 10.15406/JABB.2020.07.00238

Tristancho-Baro Ai, P. Egido, Daniel Ortega, S. Mormeneo-Bayo, A. Rezusta

{"title":"Facing COVID 19 pandemic: experience from a clinical microbiology laboratory in Spain","authors":"Tristancho-Baro Ai, P. Egido, Daniel Ortega, S. Mormeneo-Bayo, A. Rezusta","doi":"10.15406/JABB.2020.07.00238","DOIUrl":"https://doi.org/10.15406/JABB.2020.07.00238","url":null,"abstract":"Objectives: To calculate the turnaround time of results to be able in the electronic medical record. Postulate some available tools regarding laboratory management to assume full response in an increasing demand scenario. Materials and methods: Retrospective analysis of all samples reaching the lab since February 17 until May 10 was performed using LIS. Records of personnel management and equipment delivery were consulted. Time to results was measured as the difference in hours between time of analytic request and the date of result upload to the electronic medical record. Results: Time to result started at 24 hours and continually decreased over time reaching stability on week 10 around 6.5 hours. Active measurements taken fall into groups: personnel management, Laboratory schedules and technical capacity. Conclusion: Adoption of an uninterrupted sample processing method (24/7) and the implementation of high throughput systems are the best options for increasing results performance, where other measurements like redistributing and re train personnel would be more successfully implemented. Keywords: clinical laboratory services, SARS-CoV-2, medical laboratory personnel, clinical laboratory techniques, health policy, COVID-19","PeriodicalId":15033,"journal":{"name":"Journal of Applied Biotechnology & Bioengineering","volume":"36 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73387899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Changes of genetic variances and heritability by effect of selection in a Mexican local variety of Squash 墨西哥地方品种壁球遗传变异和遗传力的选择效应变化

Journal of Applied Biotechnology & Bioengineering Pub Date : 2020-10-15 DOI: 10.15406/JABB.2020.07.00237

C. Villanueva-Verduzco, José Antonio Ayala-Esteban, Evert Villanueva-Sánchez, Jaime Sahagen-Castellanos, M. G. I. Garza

{"title":"Changes of genetic variances and heritability by effect of selection in a Mexican local variety of Squash","authors":"C. Villanueva-Verduzco, José Antonio Ayala-Esteban, Evert Villanueva-Sánchez, Jaime Sahagen-Castellanos, M. G. I. Garza","doi":"10.15406/JABB.2020.07.00237","DOIUrl":"https://doi.org/10.15406/JABB.2020.07.00237","url":null,"abstract":"A local variety of squash (Cucurbita pepo L.), ‘Round Zuchinni’ type from Los Reyes Acatlixhuayan State of México, México, was simultaneously evaluated and selected. The objective was to study effect of selection on genetic variance and heritability. Additive genetic variance decreased in seven of nine traits studied: fruit height (79.93 %); fruit weight (65.72 %); fruit width (60.91 %); flesh thickness (57.66 %); flesh color (43.70 %); dry weight of seed (39.54 %); flesh flavor (16.60 %); except in width and seed length traits where it increased 63.40 % and 0.81 %, respectively. Only weight of seed had dominance genetic variance. The coefficient of additive genetic variance (CVA) fluctuated from 9.4 to 61.7 % in the first cycle, and from 9.4 to 51.8 % in the second cycle of selection-evaluation among traits. Heritability diminished in seven from nine characters. In general, the estimated genetic variances (additive and dominance) and heritability decreased as a result of combined selection of falf sib families.","PeriodicalId":15033,"journal":{"name":"Journal of Applied Biotechnology & Bioengineering","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82206936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Involvements of food grade dialdehydic pectin as cross-linker and soy protein as additive in the production of MNP-CLEA-lipase from Hevea brasiliensis 食品级二脱水果胶作为交联剂和大豆蛋白作为添加剂在橡胶树mnp - clea -脂肪酶生产中的作用

Journal of Applied Biotechnology & Bioengineering Pub Date : 2020-10-13 DOI: 10.15406/jabb.2020.07.00236

Nur Amalin Ab Aziz Al Safi, F. Yusof, Azlin Suhaida Azmi

{"title":"Involvements of food grade dialdehydic pectin as cross-linker and soy protein as additive in the production of MNP-CLEA-lipase from Hevea brasiliensis","authors":"Nur Amalin Ab Aziz Al Safi, F. Yusof, Azlin Suhaida Azmi","doi":"10.15406/jabb.2020.07.00236","DOIUrl":"https://doi.org/10.15406/jabb.2020.07.00236","url":null,"abstract":"Skim latex from Hevea brasiliensis (rubber tree) contains many useful enzymes that can be utilized to produce value-added products. Lipase recovered from skim latex serum was immobilized via cross-linked enzyme aggregates (CLEA) technology, while supported by magnetic nanoparticles termed ‘MNP-CLEA-lipase’. One of the main advantages of this method is, the biocatalyst can be easily recovered by a magnetic field for recycling. In this research, dialdehydic pectin was used as the cross-linking agent, replacing glutaraldehyde, and soy protein was used as an additive, replacing bovine serum albumin (BSA). The operating conditions for the preparation of the most active MNP-CLEA-lipase were optimized statistically. Dialdehydic pectin and soy protein showed optimum operating conditions at 180 mg/mL and 0.6% (w/v) respectively and with (NH4)2SO4 at 80% saturation, giving a Residual Activity (RA) of 154%. The optimum temperature for MNP-CLEA-lipase was at 40°C (127% RA) while the optimum pH was at 8 (127% RA). It retained 20% RA after 100 days of storage. The reusability test showed that it maintained 7% RA after the 10th cycle. This research showed two advantages; first, that value-added products can be recovered from agricultural waste, and second, by alternating to low-cost food-grade cross-linkers and additives, enhanced biocatalyst can be produced which can be safely used in the beverage and food industries","PeriodicalId":15033,"journal":{"name":"Journal of Applied Biotechnology & Bioengineering","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74304230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fast pyrolysis biochar in eucalyptus seedling production 快速热解生物炭在桉树苗木生产中的应用

Journal of Applied Biotechnology & Bioengineering Pub Date : 2020-09-29 DOI: 10.15406/jabb.2020.07.00234

S. Mazette, Marina Moura Morales, Jair v, F. Miranda, F. J. Moura, E. Andrade

引用次数: 1

Effect of silicon on tomato production in semi-controlled conditions in the Colombian Caribbean 哥伦比亚加勒比地区半受控条件下硅对番茄生产的影响

Journal of Applied Biotechnology & Bioengineering Pub Date : 2020-09-21 DOI: 10.15406/jabb.2020.07.00233

Luis Fernando Acosta Pérez, E. C. Herrera

引用次数: 0

The main protease of SARS-CoV-2 as therapeutic target to development specific drugs to treat COVID-19 SARS-CoV-2的主要蛋白酶作为治疗靶点，开发特异性药物治疗COVID-19

Journal of Applied Biotechnology & Bioengineering Pub Date : 2020-09-09 DOI: 10.15406/jabb.2020.07.00232

R. E. Silva-López

{"title":"The main protease of SARS-CoV-2 as therapeutic target to development specific drugs to treat COVID-19","authors":"R. E. Silva-López","doi":"10.15406/jabb.2020.07.00232","DOIUrl":"https://doi.org/10.15406/jabb.2020.07.00232","url":null,"abstract":"The pandemic disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) denominated COVID-19 is an important problem of world public health because do not have specific drugs and vaccines available to control the disease progression and the rapid virusspreading. SARS-CoV-2 genome resemble to other SARS-CoV andMERS-COVcoronaviruses genomes, thus drugs developed for SARS and MERS treatment may be used for COVID-19. The aim of this work is discuss the molecular target of SARS-CoV-2, the main protease, a chymotrypsin-like named as 3CLpro, for a rational devolpment of specific molecules anti-SARS-CoV-2 with broad-spectrum anti-coronavirus activities.This study is a literature mini review using 47 articles obtained from Public Medline and Science directmainly from this year 2020. The coronaviruses infection is mediated by spike protein from viral capsid which bind to ACE2 receptor in the host cells which endocytes the viruses RNA, which is translated to proteins that are cleavead by proteases, mainly the 3CLpro. This enzyme is crucial to SARS-CoV-2 replication and its inhibition can prevent the virus replication. Thus, it is an important coronaviruses target because the amino acid sequence of 3CLpros from SARS, MERS and SARS-CoV-2 have high similarity. In silico studies have been demonstrated that synthetic small molecules, natural products, peptidomimetic inhibitors, and HIV inhibitors had different docking scores with SARS-CoV-23CLpro. However, peptidomimetic inhibitors seems to be better inhibitors to this protease and promise dugs to treat COVID-19 and other diseases caused by coronaviruses","PeriodicalId":15033,"journal":{"name":"Journal of Applied Biotechnology & Bioengineering","volume":"34 2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80080457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Antimicrobial and antioxidant effects of phlorotannin coating in spoilage prevention of tomato during storage 绿单宁涂层在番茄贮藏过程中的抗菌和抗氧化作用

Journal of Applied Biotechnology & Bioengineering Pub Date : 2020-08-31 DOI: 10.15406/jabb.2020.07.00231

Sreekala Kannikulathel Gopidas, Nagaraj Subramani

{"title":"Antimicrobial and antioxidant effects of phlorotannin coating in spoilage prevention of tomato during storage","authors":"Sreekala Kannikulathel Gopidas, Nagaraj Subramani","doi":"10.15406/jabb.2020.07.00231","DOIUrl":"https://doi.org/10.15406/jabb.2020.07.00231","url":null,"abstract":"The microbial flora present on vegetables and fruit is commercially significant as they cause spoilage and potential health risks in consumers. Synthetic chemicals used to combat food microbes posed serious health and environmental hazards leading to the search for natural alternatives. In this study, phlorotannins from three Indian brown seaweeds, Sargassum myriocystum, Turbinariaornata, and Padina tetrastromatica were evaluated for antimicrobial, antioxidant, and fruit spoilage prevention potentials. The crude phlorotannin of Padina with the highest content (0.114±0.0005g kg-1) and antioxidant activity (92.65±3.43%) was purified by column chromatography. The Padina water fraction I with the highest antioxidant potential was characterized by Fourier Transform Infra-Red spectroscopy and a 5 % solution was applied as a coating on tomatoes segregated into control chilled (CC), treated chilled (TC), control non-chilled (CNC) and treated non chilled (TNC). Spoilage parameters were studied over twenty days, using a swab and spread plate methods to assess the microbial load on tomatoes. Compared to the control sets, the treated sets exhibited a low number of microbial colonies and retained a fresh look and yellow color. The antibacterial and antioxidant properties of phlorotannin coating as a natural alternative for preservatives, effectively reduced bacterial load, inhibiting the early decay of tomatoes","PeriodicalId":15033,"journal":{"name":"Journal of Applied Biotechnology & Bioengineering","volume":"387 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79570635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plant protease inhibitors as specific strategies against cancer cells 植物蛋白酶抑制剂作为抗癌细胞的特异性策略

Journal of Applied Biotechnology & Bioengineering Pub Date : 2020-08-24 DOI: 10.15406/jabb.2020.07.00230

Érika Maria Gomes Ferreira Teixeira, Danilo Oliveira, R. E. Silva-López

引用次数: 0

Effect of temperature and moisture on the uniformity of delivered dose, emitted dose and fine particle fraction for three different DPI formulations 温度和湿度对三种不同DPI配方的给药剂量、发射剂量和细粒分数均匀性的影响

Journal of Applied Biotechnology & Bioengineering Pub Date : 2020-08-20 DOI: 10.15406/jabb.2020.07.00229

S. Bhope, Priyanka Gondhale, Shrikant G. Kulkarni, B. Musmade, P. Kolsure, S. Dhavale, Sriram Padmanabhan

{"title":"Effect of temperature and moisture on the uniformity of delivered dose, emitted dose and fine particle fraction for three different DPI formulations","authors":"S. Bhope, Priyanka Gondhale, Shrikant G. Kulkarni, B. Musmade, P. Kolsure, S. Dhavale, Sriram Padmanabhan","doi":"10.15406/jabb.2020.07.00229","DOIUrl":"https://doi.org/10.15406/jabb.2020.07.00229","url":null,"abstract":"The development of a stable dry powder inhalation (DPI) product with the delivery of consistent fine particle fraction until the product expires is challenging for many drug companies. The main purpose of this study was to investigate the influence of both temperature and humidity on the uniformity of delivered dose, emitted dose and fine particle fraction on three different DPI products in two different packs viz. HDPE container and Alu Alu blister during the stability studies. The study has been performed on Budesonide 400 mcg, Formoterol fumarate dihydrate 12 mcg and Tiotropium bromide 18 mcg DPI products. The uniformity of delivered dose (UDD) is calculated by using the Dosage Unit Sampling Apparatus (DUSA) and the emitted dose (ED) and fine particle fraction (FPF) are calculated by using the Next Generation Impactor (NGI). All three products have shown no significant reductions in the UDD, Formoterol fumarate showed a significant reduction in ED value and all of them showed a significant reduction in the FPF values. The reduction in FPF was more in Alu Alu blisters as compared to HDPE containers.The outcome of the study could be extrapolated to other DPI formulations.","PeriodicalId":15033,"journal":{"name":"Journal of Applied Biotechnology & Bioengineering","volume":"44 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90788119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Polymerase chain reaction: a creative review 聚合酶链反应:一个创造性的评论

Journal of Applied Biotechnology & Bioengineering Pub Date : 2020-07-23 DOI: 10.15406/JABB.2020.07.00228

Iffat Nazir, Hafiz Zaid Mahmood, S. Mustafa

引用次数: 0