聚合酶链反应:一个创造性的评论

Iffat Nazir, Hafiz Zaid Mahmood, S. Mustafa
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引用次数: 0

摘要

在分子生物学中,聚合酶链反应(PCR)是一种科学技术,用于产生单个特定DNA序列的数千到数百万份拷贝,以将一个DNA片段的单个或几个拷贝放大几个数量级。在多种应用中,PCR是一种在医学和生物学研究实验室中使用的常见且经常至关重要的实践,用于多种疾病的诊断和调查。PCR主要涉及三个主要步骤。变性,退火,延伸。PCR不仅可以用于检测人类基因组,还可以用于检测病毒和细菌的基因组。PCR在法医实验室特别有用,因为只需要非常少量的原始DNA。在癌症的发展中,基因通过PCR被牵连
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Polymerase chain reaction: a creative review
In molecular biology, a scientific technique PCR (polymerase chain reaction) is used to generate thousands to millions of copies of a single particular DNA sequences to amplify a single or few copies of a piece of DNA across several orders of magnitude. For multiple applications, PCR is an ordinary and often vital practice used in medicinal and biological research labs and is used for diagnosis and investigation of multiple diseases. In PCR mainly three major steps are involved. Denaturation, annealing, and extension. PCR can be used to detect not only the human genome but also the genome of viruses and bacteria. PCR is especially useful in forensic laborites because a very small amount of original DNA is required. In the development of cancer, genes have been implicated through PCR
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