Journal of Applied Biotechnology & Bioengineering最新文献

{"title":"Micropropagation of selected materials of Opuntia ficus indica L through culture in vitro of areols","authors":"Rodriguez-de la OJL, Ramirez-Pantoja Pe","doi":"10.15406/jabb.2020.07.00225","DOIUrl":"https://doi.org/10.15406/jabb.2020.07.00225","url":null,"abstract":"Globally Mexico has been characterized by being a very important country because it has a huge biodiversity that houses in terms of its biotic resources of both plants and animals, the above also confers an important endemism, which according to data from the National Commission for the Knowledge and Use of Biodiversity (CONABIO) are concentrated around 9500 endemic species, of which the family of cacti stands out. One of the most important species of this family is the nopal (Opuntia sp) of which Mexico is the leader in the production of nopal vegetables. In 2010, according to data from the National System of Phylogenetic Resources for Food and Agriculture (SINAREFI) 12,472.59 hectares were registered from which 723,815.23 tons of fresh product were obtained. Which makes the country the main exporter of nopal, making it a very important crop.1–3","PeriodicalId":15033,"journal":{"name":"Journal of Applied Biotechnology & Bioengineering","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75463480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of omega-3 on muscle and non-muscle cells on a 2D fibronectin and in 3D fibrin constructs","authors":"Sozzi Simon, Souza Danilo, Sidhu Karandeep, Garcia Victor, Tawil Bill","doi":"10.15406/jabb.2020.07.00224","DOIUrl":"https://doi.org/10.15406/jabb.2020.07.00224","url":null,"abstract":"Human Foreskin Fibroblasts (HFF-1) and L6 muscle cells were treated in several conditions to examine the proliferation and adhesion of cells using different extracellular matrix proteins with different concentrations. These proteins consist of Collagen & Fibronectin with concentrations of 5ug/ml and 10ug/ml respectfully. The experiment was conducted and focused on how HFF-1 and L6 cells proliferate in two different conditions over a 7-day period and the results were recorded on Days 1, 3 & 7 (n=9). Different concentrations of Fibronectin and Collagen altered the proliferation of HFF-1 cells over a 7-day period. Once it was identified that the best proliferation of HFF-1 and L6 was the Fibronectin 10ug/mL coating, we used the same protein matrix to study the effects of different concentrations of Generic Omega-3 in cell adhesion and proliferation. Throughout the study, it is observed that proliferation is not proportional to the Omega-3 concentration, meaning in this study 1 mg/ml had the lowest proliferation. In conclusion from the first study, higher concentrations of Generic Omega-3 decreases the proliferation of HFF-1 and L6 cells. This is a known characteristic as Omega-3 inhibits cell proliferation by prolonging the G1 phase but did not arrest the G0-to-G1 or G1-to-S transitions. The prolonged G1 phase in fad3b ESCs was probably induced by downregulation of Cdk4 expression via p21 upregulation. In comparison to the Generic product of Omega-3 we decided to incorporate the Synthetic substance of Omega-3 into the study. The overall goal of this experiment was to observe the proliferation and adhesion of HFF-1 and L6 cells in artificially created media containing pure Synthetic Omega-3 fatty acids. This experiment was repeated three times (n=9) to gain valuable conclusive data which could be used to design future experiments. An additional experiment was specifically designed to see how HFF-1 and L6 cells migrate through a thick 3-D layer of fibrin. Fibrin is a substance formed through catalytic conversion of coagulation constituents: fibrinogen and thrombin. This experiment was conducted, and the results were taken for Day 1 and Day 7. Our results remained consistent in comparison to prior studies. With the use of higher concentrations of Synthetic Omega-3 there was a decrease in proliferation for both HFF-1 and L6 cells.","PeriodicalId":15033,"journal":{"name":"Journal of Applied Biotechnology & Bioengineering","volume":"44 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89926084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Moulage in clinical simulation: step-by-step making of a third-degree burn","authors":"Danielle R dos S Carvalho, Patrícia da S Klahr, Nicolas Murcia, M. L. Medeiros, Francisco GMRC Neto","doi":"10.15406/jabb.2020.07.00223","DOIUrl":"https://doi.org/10.15406/jabb.2020.07.00223","url":null,"abstract":"Relevant components are necessary for high-fidelity simulation, including scripted scenarios, well-trained simulation educators and “actors,” appropriate mannequins, and realistic environments. Moulage can be yet another key element when used properly. In health courses, moulage can be worked on using various themes. One of them worth working on is burn. This is considering that efficient care requires well-trained health professionals. The aim of this paper is to describe the preparation of moulage that represents a realistic, easy-to-perform third-degree burn using easily accessible and inexpensive materials. First, the researchers began with the search for specific literature, followed by the necessary material selection, and defining a model for the simulated burn. The moulage was executed in approximately 60 minutes. The step-by-step technique has been described and photographed, using low-cost, easily accessible materials to allow moulage to be considered and applied more often in the scenarios of various health-related areas. We believe our model can be used in a variety of simulation scenarios, making it relevant to educators and student learning.","PeriodicalId":15033,"journal":{"name":"Journal of Applied Biotechnology & Bioengineering","volume":"78 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81466439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A review on lignin sources and uses","authors":"F. J. Gomes, Rafael Eloy de Souza, E. O. Brito, R. Lelis","doi":"10.15406/jabb.2020.07.00222","DOIUrl":"https://doi.org/10.15406/jabb.2020.07.00222","url":null,"abstract":"The efficient use of renewable resources has become a driving force for the worldwide industry aiming to improve the competitiveness. Considering the available natural raw materials, the lignin present in the lignocellulosic biomass such as trees, is the unique natural polymer that presents aromatic rings in its constitution. In this way, this review details the structure of native lignin as well as the technical lignin, including information on the characteristics that this polymer must have for the most promissory applications as feedstock for bioproducts. Approximately 50 million tons of lignin are produced worldwide annually, of which 98% to 99% is incinerated to produce energy and steam in the pulp mills. Only a small fraction of the lignin, derived mainly from the sulfite pulp mill is recovered commercially in a biorefinery concept. There are many opportunities for producing high value-added products from technical lignin, mainly considering the pulp mills growing, and environmental restrictions using non-renewable raw materials. The currently trend have shown that technical lignin sources may also be used as feedstock for phenol derived products, technical carbons, fuels, and adhesives. On the other hand, there are some technological hurdles must be overcome to make these uses feasible.","PeriodicalId":15033,"journal":{"name":"Journal of Applied Biotechnology & Bioengineering","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83679929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Randomized study of the effects of quantum resonance technology application on human psychophysiological parameters","authors":"Korotkov Kg, Churganov Oa, Gavrilova Ea","doi":"10.15406/jabb.2020.07.00221","DOIUrl":"https://doi.org/10.15406/jabb.2020.07.00221","url":null,"abstract":"Objectives: The aim of this study was to demonstrate the beneficial effect of the Quantum Resonance Technology Yippi Wave 3.0 mobile application on the human psychophysiology. Design: An open, randomized, placebo - controlled trials for two weeks. Participants were measured initially, randomly divided into two groups (test and control) and measured two weeks later after listening to programs twice a day: experimental group - Yippi Wave 3.0 audio mobile application; control group - classical music. Subjects: 50 apparently healthy adults, age 22 – 56 aged, 18 men and 32women, randomly divided into two groups of 25 people. Randomization was based on the principle of random numbers. The study protocol was approved by the ethics committee of the North-Western Medical University, St. Petersburg, Russia. All participants signed an informed consent form, where a written and oral explanation of the research protocol was provided. Outcome measures: Physical examination by a therapist, psychological testing, rhythmocardiography, biofield parameters analysis. Results: Participants using Quantum Resonance Technology (Yippi Wave 3.0) apps for two weeks demonstrated positive changes of psychophysiology parameters, while the participants in the control group demonstrated a negative trend of these parameters. Conclusions: Quantum Resonance Technology (Yippi Wave 3.0) applications have wellness and energized effects on humans","PeriodicalId":15033,"journal":{"name":"Journal of Applied Biotechnology & Bioengineering","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74219960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Public food policies: epistemological and systemic restructuring","authors":"Salatiel Avila Serrato","doi":"10.15406/jabb.2020.07.00219","DOIUrl":"https://doi.org/10.15406/jabb.2020.07.00219","url":null,"abstract":"The redefinition of public policies food should clarify the corresponding analytical approaches to a broader, concerning the meeting the food needs of individuals, being feasible towards the social and natural intone of these; generating a paradigmatic release of the current system.Implicit this parameter, the epistemological knowledge in one, not oppressive, liberator sense contributes to the decolonization of ideologies rooted consumerist, conducive to the deterioration of these environments’ wellness food and carecientes systems axes of food law, concerning a universal right. Thus, the sense of diversification of food public policies must recreate the evolving needs of welfare in a systemic process of promotion, prevention, correction and creating food Entonox epistemological rather than oppressive colonization","PeriodicalId":15033,"journal":{"name":"Journal of Applied Biotechnology & Bioengineering","volume":"47 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84008038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Breast and ovarian cell behavior in the presence of contraceptive ingredients","authors":"2020, MedranoSusana, Sidhu Karandeep, Valdivia, Ashley Lauren, TawilBill","doi":"10.15406/jabb.2020.07.00218","DOIUrl":"https://doi.org/10.15406/jabb.2020.07.00218","url":null,"abstract":"Breast cancer is the most frequently diagnosed cancer and the second leading cause of cancer death amongst women in the United States. Breast cancer growth can be fueled by estrogen, progesterone, and growth hormones such as HER2/neu gene. The ovaries are the key source of Estrogen and Progesterone. Different studies and analysis data on over 150,000 reveal a 7% increase in breast cancer rates when women are on contraceptives. The overall purpose of this study was to evaluate the behavior of breast and ovarian cells in the presence of common contraceptive ingredients Ethinyl Estradiol (EE) and Levonorgestrel (LNG). Both cell lines were evaluated at high-dose concentrations of EE and LNG as well as serum-level concentrations comparable with those of approved contraceptives. It was hypothesized that Levonorgestrel and Ethinyl Estradiol in contraceptives could have an oncogenic effect in breast and ovarian cells. Results showed that high concentrations of EE dramatically decrease the proliferation rate of both ovarian and breast cells. On the other hand, cells treated with serum-level concentrations of EE increased proliferation in both cell lines. Breast and Ovarian cells treated with high concentrations of LNG displayed a slight/modest decrease in proliferation respectively. Ovarian cells treated with serum-level concentrations of LNG found on contraceptive users displayed an increased proliferation rate if treated individually and/or in combination with EE. Breast cells treated with serum-level concentrations of LNG did not exhibit a notable impact in proliferation, however, when LNG was combined with EE at serum-level concentrations; a decreased in proliferation was observed","PeriodicalId":15033,"journal":{"name":"Journal of Applied Biotechnology & Bioengineering","volume":"36 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86692380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of anti-inflammatory agents (glucosamine sulphate and aloe vera) on mouse mesenchymal stem cells","authors":"Easwar Kumar Vigneshwari, Nambiar Roshni, Sidhu Karandeep, Tawil Bill","doi":"10.15406/JABB.2020.07.00213","DOIUrl":"https://doi.org/10.15406/JABB.2020.07.00213","url":null,"abstract":"Use of stem cells for treating degenerative diseases like osteoarthritis has been gaining popularity. Glucosamine Sulphate is a nutritional supplement used for relieving inflammation during Osteoarthritis. Aloe vera juice is a widely consumed drink used in traditional practices to help reduce inflammation. In this experiment, the effect of Glucosamine Sulphate and Aloe vera on 2D and 3D proliferation of MSC cells was studied. Initial study to determine a suitable matrix for growing MSC cells was performed by growing the cells on 5mg/ml and 10mg/ml collagen and fibronectin coated wells for seven days. Cells adhered the least on fibronectin 10ug/ml and cells proliferated the highest on fibronectin at 10ug/ml, thus establishing fibronectin 10ug/ml as the matrix for the study. The effect of Glucosamine Sulphate on 2D proliferation of MSC cells growing on 10ug/ml fibronectin matrix was studied by exposing the cells to 0mg/ml, 0.1mg/ml, 0.5mg/ml and 1 mg/ml of Glucosamine Sulphate for seven days. The cells adhered the least on 0.5mg/ml and proliferated the highest on 0.5mg/ml as well (p<0.05). The effect of Aloe vera on MSC cells grown on fibronectin at 10ug/ml was studied by using concentrations of 0%, 2%, 5% and 10% Aloe vera juice for seven days. It was found that cell proliferation radically decreased in all conditions after Day 4 and the least proliferation was observed at 10%. 3D proliferation of MSC cells in a fibrin construct was studied with 0.1 mg/ml of Glucosamine Sulphate and 2% and 5% Aloe vera juice. It was observed that 5% Aloe vera juice allowed highest proliferation of cells, followed by 0.1 mg/ml Glucosamine Sulphate and 2% Aloe vera. This indicated that further analysis with 2% and 5% Aloe vera along with 0.1mg/ml as threshold could help identify an optimum dose range for uptake of Glucosamine Sulphate with Aloe vera juice to enhance relief from inflammation during osteoarthritis","PeriodicalId":15033,"journal":{"name":"Journal of Applied Biotechnology & Bioengineering","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75302725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Process for obtaining synthetic foam with aluminum matrix Al-5052 by method of tixoinfiltration","authors":"J. Moreno, Rafael Claudiano de Moraes, Joao Paulo de Oliveira Paschoal, Rodrigo Henrique Lopes","doi":"10.15406/JABB.2020.07.00210","DOIUrl":"https://doi.org/10.15406/JABB.2020.07.00210","url":null,"abstract":"The metallic foams have lower density, and important physical, mechanical, thermal and acoustic properties, has been highlighted in its use in innovative technologies. As a subgroup of the class of metallic foams, the syntactic metallic foams which consists of the addition of hollow ceramic balloons in a metallic matrix in aluminum Al-5052 by process of tixoinfiltration. The microstructure was studied by optical and electron microscopy extended by energy-dispersive X-ray spectrometry, while the basic mechanical properties were mapped by standardized compression tests. Therefore, a syntactic foam design was developed in this work, where we obtained a 30% reduction in density and a reasonable increase in hardness due to the homogeneous dispersion of the ceramic balloons","PeriodicalId":15033,"journal":{"name":"Journal of Applied Biotechnology & Bioengineering","volume":"103 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85842910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Remote detection of music influence with physical sensor","authors":"Konstantin G. Korotkov","doi":"10.15406/JABB.2020.07.00209","DOIUrl":"https://doi.org/10.15406/JABB.2020.07.00209","url":null,"abstract":"Goals and objectives of the study: The goal of this paper was to study the possibility of remote detection of electronic music influence with a physical sensor. Study design: Bio-Well technology with a special environmental sensor was used in the study. This sensor was developed for the analysis of the electromagnetic fields and was shown to respond to the changed in the environmental parameters. Signal of a sensor was detected for an hour in the background mode after this music was turned on for 20 minutes, and then after-effect was detected for about one hour. In experiment 5 different files of the modern electronic music “Yippi Wave 3.0” (https://yippiweb.com/what-is-wave) in *wav format and several files of Handel music have been used. Results: The statistically significant changes of sensor parameters to “Yippi Wave 3.0” music were detected in several time-series, while no response to Handel music. Conclusions: This study should be considered a pilot study and the results are preliminary. We need to conduct experiments with different sources of both acoustic and electromagnetic signals and the possibility of their registration by the Bio-Well device","PeriodicalId":15033,"journal":{"name":"Journal of Applied Biotechnology & Bioengineering","volume":"32 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89446773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}