S. Kamiński, T. Zabolewicz, K. Oleński, A. Babuchowski
{"title":"Long-term changes in the frequency of beta-casein,\u0000kappa-casein and beta-lactoglobulin alleles\u0000in Polish Holstein-Friesian dairy cattle","authors":"S. Kamiński, T. Zabolewicz, K. Oleński, A. Babuchowski","doi":"10.22358/jafs/157531/2023","DOIUrl":"https://doi.org/10.22358/jafs/157531/2023","url":null,"abstract":". The purpose of the study was to analyse changes in the frequency of genotypes and alleles of beta-casein ( CASB ), kappa-casein ( CSN3 ) and beta-lactoglobulin ( LGB ) loci among bulls (used for insemination in 1991–2021) and cows (milked in 2014 and 2021). The analysis included 1812 bulls and 2628 cows of the Polish Holstein-Friesian breed. Genotypes of three milk protein loci were identified using PCR-RFLP/ACRS method (bulls) or the Illumina Bovine MDv2 Chip (cows). For bulls used in insemination between 1991 and 2021, the frequency of the desirable CSN3 allele B and allele A2 at the CASB locus increased by 44% and 8%, respectively. In cows, the frequency of allele B of CSN3 and allele A2 of CASB increased over the past seven years by 11% and 7%, respectively. The frequency of LGB allele B decreased in cows from 47% to 41%. An increase in the frequency of the rarest allele E at the CSN3 locus in bulls was also observed (from 2% to 7%). The number of animals carrying the most desirable combination of CASB and CSN3 genotypes (A2A2 and BB) reached 4.25% and 2.8% in cows and bulls, respectively. The current results showed a slow but marked increase in the desirable alleles at casein gene loci which should improve milk quality and cheese-processing properties. This positive effect was attenuated by the simultaneous decrease in the frequency of LGB allele B.","PeriodicalId":14919,"journal":{"name":"Journal of Animal and Feed Sciences","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48372025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}