Effects of phytogenic feed on productive performance, egg quality, antioxidant activity and lipid metabolism of laying hens

. The objective of this study was to investigate the effect of different proportions of Moringa oleifera leaf powder (MOLP) and mulberry leaf powder (MLP) on the performance of laying hens. The use of MOLP and MLP as animal feed can meet the nutritional requirements of livestock and improve antioxidant status. Combinations of different feeds could produce a synergistic effect in animals. Two hundred and ten birds were randomly divided into three groups. The control group was fed a basal diet and the treatment groups received a basal diet supplemented with 2.5% MOLP + 2.5% MLP and 5% MOLP + 2.5% MLP, respectively. The results showed that the supplementation of 5% MOLP + 2.5% MLP significantly decreased the laying rate, albumen height and Haugh unit. Yolk colours were significantly more intense after MOLP and MLP supplementation. Significant decreases in abdominal fat index were observed in the 5% MOLP + 2.5% MLP group. The levels of superoxide dismutase 2 ( SOD2 ) mRNA expression were significantly increased, while those of apolipoprotein B ( APOB ) were significantly reduced, as determined by qRT-PCR analysis. The combined administration of MOLP and MLP could have regulated the antioxidant status and lipid metabolism by affecting SOD2 and APOB gene expression. A supplementation level of 2.5% MOLP + 2.5% MLP was recommended.