Jennifer E Earing, Kinze Haywood, Keith Stewart, Joshua C McCann
{"title":"PSIV-A-6 The effects of botanical composition of pasture on fecal egg counts in grazing sheep","authors":"Jennifer E Earing, Kinze Haywood, Keith Stewart, Joshua C McCann","doi":"10.1093/jas/skaf102.391","DOIUrl":"https://doi.org/10.1093/jas/skaf102.391","url":null,"abstract":"Grazing sheep are prone to harboring gastrointestinal parasites that often impact animal health and productivity. The objective of this study was to evaluate the effect of botanical composition of pasture on parasitic fecal egg counts in grazing sheep. Dorset × Suffolk crossbred ewes (n=34; BW=75.3 kg) and their offspring (n=57; BW=23 kg) were used. Four weeks prior to the start of the study, all ewes were orally dewormed with cydectin. Ewes were stratified by weight and assigned (with their lambs) to one of two pastures mixes: GM (Grass Mix, 47% orchard grass, 30% white clover 13% ryegrass, 10% bromegrass) or LM (Legume Mix, 45 % ryegrass, 28% red clover, 27% white clover). Each 0.30-hectare pasture was subsequently divided into four grazing paddocks through which each group was rotationally grazed. Sheep rotated between paddocks every 4-5 d to prevent over grazing. Forage samples, collected prior to sheep entering a new paddock, were analyzed for crude protein (CP), total digestible nutrients (TDN), acid detergent fiber (ADF), neutral detergent fiber (NDF), and mineral content. At the end of the 22-d-grazing period, fecal egg counts (FEC) were conducted on ewes and lambs to quantify stomach worm, coccidia, and nematodirus, burden using the McMaster method. Differences were identified in forage quality. CP and copper levels were higher in LM pastures (P< 0.01), NDF was lower in LM pastures (P≤0.001), and manganese was higher in GM (P≤0.001). There were no differences in TDN, ADF, or zinc content. Stomach worms, coccidia, and nematodirus were identified in sheep in both pasture mixes. FEC for stomach worms was 1537 and 4347 epg for the GM and LM pastures, respectively (P=0.1872). Coccidia load in sheep in the GM pastures averaged 3315 epg while those in LM pastures averaged 4568 epg (P >0.05). The Nemotadirus load averaged 35 epg (GM) and 8 epg (LM; P> 0.05). The FEC for stomach worms was 1537 and 4347 epg for the GM and LM pastures, respectively (P >0.05). When separated by age, there were no differences in stomach worms or nematodirus among ewes on GM or LM (P>0.01), however, ewes on GM had fewer coccidia (P=0.02). There were no differences in FEC in lambs on GM or LM pasture for any of the parasites evaluated (P>0.05). The percentage of sheep above 1,000 epg was calculated for each of the parasites; no differences were observed due to pasture mix. The proportion of sheep with a burden greater than 1,000 epg was 36% (GM) and 48% (LM) and 55% (GM) and 58% (LM), for stomach worms and coccidia, respectively. No sheep had a nematodirus burden greater than 1,000 epg. Data from the current study indicates that botanical composition does not significantly impact parasite burden in sheep.","PeriodicalId":14895,"journal":{"name":"Journal of animal science","volume":"9 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144104572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"PSIII-10 Effect of different copper sources on growth performances in nursery pigs","authors":"Daniel G Rosales, Ryan S Samuel, Eric M Weaver","doi":"10.1093/jas/skaf102.332","DOIUrl":"https://doi.org/10.1093/jas/skaf102.332","url":null,"abstract":"Copper plays a significant role in the growth performance of pigs, influencing feed efficiency and nutrient retention. Recent studies indicate that the source and concentration of copper can markedly affect growth metrics such as average daily gain (ADG) and feed conversion ratios. A total of 1,144 newly weaned pigs (initial body weight (BW) 5.9 ± 1.2 kg) were used to evaluate the effects of copper (Cu) supplementation from two tribasic copper chloride (TBCC) sources and one chelated copper (cCu) source on growth performance of nursery pigs. Pigs were blocked by BW and housed 26 pigs per pen in 44 pens in a randomized block design. Each pen was assigned to one of four dietary treatments within each block that consisted of: 1) Control (corn-soybean meal (SBM) diet which was formulated to provide 12 ppm of Cu from TBCC), 2) Control + TBCC #1 providing 150 ppm of Cu, 3) Control + cCu providing 150 ppm of Cu, and 4) Control + TBCC #2 providing 150 ppm of Cu. Pigs received 2.5, 5.5, and 16 kg and the feeding phases lasted 12, 9, and 14 days for Phases 1, 2, and 3, respectively. Feed remaining each week was calculated using a calibration curve based on the distance from the top of the feeder to the top of the feed and the feed density. Additionally, total pen weights were measured each week using a scale to capture the weight of all pigs in each pen. In Phase 1 (0-12 d), ADG (200 vs. 170 g/d) and ADFI (230 vs. 210 g/d) were improved (P < 0.01) by Cu compared to Control. Also, TBCC #1 had a significantly higher gain:feed ratio (G:F) than Control (0.82 vs. 0.62; P = 0.02) in Phase 1. In Phase 2 and Phase 3, no significant differences (P > 0.05) were observed among treatments in ADG, ADFI, nor G:F. Overall, all Cu supplementation showed equivalent results throughout the trial, with only slight numerical differences between each other. These results indicate that growth performance of pigs was influenced influenced by increasing feed intake due to copper inclusion.","PeriodicalId":14895,"journal":{"name":"Journal of animal science","volume":"23 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144104573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ty J Schoelerman, John P Hutcheson, Lee-Anne J Walter, Wade T Nichols, Ben P Holland, Alyssa B Word
{"title":"120 The effect of four different implant programs on feedyard performance, health, and carcass characteristics of native light weight beef heifers","authors":"Ty J Schoelerman, John P Hutcheson, Lee-Anne J Walter, Wade T Nichols, Ben P Holland, Alyssa B Word","doi":"10.1093/jas/skaf102.242","DOIUrl":"https://doi.org/10.1093/jas/skaf102.242","url":null,"abstract":"Due to new reimplant restrictions, delaying implant administration after arrival could prevent inadequate implant coverage at the end of the feeding period. Therefore, the objectives of this study were to determine the effects of a traditional reimplant program [Synovex Choice (100 mg trenbolone acetate and 14 mg estradiol benzoate) on arrival reimplanted at 110 days with Synovex Plus (200 mg trenbolone acetate and 28 mg estradiol benzoate) (CHPL)] relative to an extended release implant [Revalor XH (200 mg trenbolone acetate and 20 mg estradiol) (XH)] administered on arrival (XH0) or delayed 40 (XH40) or 80 d (XH80) to determine an ideal implant window in heifers fed 219 d. A total of 2,800 heifers (initial BW = 254 ± 27.24 kg.) were enrolled, blocked by time of arrival and randomized to treatment. Dry matter intake was reduced (P = 0.02) in XH80 heifers, greatest in XH0 and CHPL heifers with XH40 heifers not different. There were no differences in final BW, ADG or G:F between treatments (P ≥ 0.12). Hot carcass weight did not differ (P ≥ 0.19) between treatments, whereas ribeye area was greater (P = 0.02) when heifers were administered XH40 (83.42 cm2) or CHPL (83.35 cm2) vs XH80 (81.61 cm2); XH0 (82.64 cm2) did not differ from other treatments. Implant treatment did not affect backfat and EBF (P ≥ 0.10); however, marbling score was lower (P = 0.04) for CHPL (455) heifers vs. XH80 (478) while XH0 (470) and XH40 (459) were not different than other treatments. Percentage of carcasses grading USDA Prime was greatest (P < 0.01) for XH80 (5.78%), vs XH0 (2.55%) and CHPL (2.72%). Percentage of carcasses grading Choice was also reduced (P = 0.05) for CHPL (65.89%) compared to all other treatments (76.11, 71.94 and 74.84% for XH0, XH40 and XH80, respectively). Conversely, the percentage of carcasses grading Select was greatest (P < 0.01) for CHPL compared to all XH treatments. There were no differences among treatments in percentage of carcasses classified YG 1, 3, or 5 (P ≥ 0.20). However, percentage of carcasses classified YG2 was greatest (P = 0.02) in CHPL (31.74%), least in XH0 (24.95%) and XH40 (26.83%), with XH80 (27.39%) not different than other treatments. Percentage of carcasses classified YG4 was greater (P = 0.01) for XH80 (25.74%) and XH0 (24.68%) compared to CHPL (19.21%) and XH40 (19.58%). Revalor-XH on arrival or delayed 40, or 80d did not affect live growth performance of heifers, although DMI was reduced when Revalor-XH was administered on day 80. A reimplant program of Synovex Choice followed by Synovex Plus did not affect live performance or HCW but resulted in leaner carcasses and reduced quality grade compared to an on-arrival or delayed administration of Revalor-XH.","PeriodicalId":14895,"journal":{"name":"Journal of animal science","volume":"2 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144104592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Diego A Lopez Diaz, Matt D Miesner, Jordan T Gebhardt, Hans H Stein, Charles R Stark, Chad B Paulk
{"title":"216 Effect of conditioning time on standardized ileal digestibility of amino acids and total tract digestibility of energy in diets fed to growing pigs","authors":"Diego A Lopez Diaz, Matt D Miesner, Jordan T Gebhardt, Hans H Stein, Charles R Stark, Chad B Paulk","doi":"10.1093/jas/skaf102.058","DOIUrl":"https://doi.org/10.1093/jas/skaf102.058","url":null,"abstract":"Two experiments were conducted to determine the effect of conditioning time prior to pelleting on digestibility of energy and AA in diets fed to growing pigs. For both experiments, the dietary treatments consisted of a mash diet and 3 separate pelleted diets that were conditioned for 30, 60, or 180s prior to pelleting. For Exp 1, Twelve barrows (DNA 200 x 400, DNA; BW: 34.1±1.03 kg) were allotted to a 4 x 3 balanced incomplete Latin square design with 4 treatments and 3 periods, for a total of 9 replicates per treatment. Pigs were housed individually in metabolic crates equipped with a feeder, a drinker, and partial diamond-slatted floor. For each experimental period, the first 5d were considered adaptation and the next 5d were collection. During collection, a screen and urine pan were installed underneath the floor of the crate to allow for the total collection of feces and urine samples. For Exp. 2, twelve barrows (DNA 200 x 400, DNA; BW: 44.9±2.70 kg) were allotted to a replicated 4 x 4 balanced Latin square design with 4 treatments and 4 periods, for a total of 12 replicates per treatment. Pigs were housed individually in the same metabolic crates as Exp. 1. Each experimental period consisted of 5d of adaptation and 2d of ileal collection. For Exp.1, conditioning diets for 60s increased (P < 0.05) DE and ME compared to the mash control and diets conditioned for 30s; however, conditioning diets for 180s decreased (P < 0.05) DE and ME compared to all other treatments. For Exp. 2, a treatment effect (P < 0.001) was observed for SID of CP, all indispensable AA, all dispensable AA, and total AA. There was no evidence of difference for Lys, Met and Trp. The SID of CP, Arg, Leu, Thr, Ala, Ser, and Tyr was greater (P < 0.05) in the pelleted diets compared to the mash diet. For His, Ile, Phe, Val, Asp, and Glu, mash diets had decreased (P < 0.05) SID compared to the pelleted diets conditioned for 60s and 180s. However, there was no evidence of difference in SID of Val for any of the conditioning lengths. For SID of Cys, mash diets had decreased (P < 0.05) SID compared to the pelleted diets conditioned for180s and there was no evidence of difference between the mash diets and pelleted diets conditioned for 30s or 60s. These results indicated that increasing conditioning time up to 180s did not negatively impact AA digestibility, but improved AA digestibility for certain AA. It was also demonstrated that conditioning the diets for 60s optimized the DE and ME of the diet, while conditioning up to 180s decreased the DE and ME of the diet.","PeriodicalId":14895,"journal":{"name":"Journal of animal science","volume":"8 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144104593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jessica P Acosta, S Maria S Mendoza, John K Htoo, Hans H Stein
{"title":"136 Nitrogen retention was not different in pigs fed diets supplemented with 65 parts of DL-Methionine or 100 parts of MHA-Ca","authors":"Jessica P Acosta, S Maria S Mendoza, John K Htoo, Hans H Stein","doi":"10.1093/jas/skaf102.107","DOIUrl":"https://doi.org/10.1093/jas/skaf102.107","url":null,"abstract":"An experiment was conducted to test the hypothesis that pigs fed a low crude protein diet supplemented on a product-to-product (wt-wt) basis with 65 parts of DL-methionine (DL-Met, 99%) or 100 parts of the calcium salt of the hydroxy analog of DL-Met (MHA-Ca, 84%) will not have different N retention. A mash Met-deficient basal diet [0.23% standardized ileal digestible (SID) Met and 0.55% SID Met + Cys], which was 19% below the requirement for 11 to 25 kg pigs was formulated. Four additional diets were prepared by supplementing the basal diet with 0.030 or 0.090% DL-Met, or 0.046 or 0.138% MHA-Ca. The greatest level of Met supplementation (0.64% SID Met + Cys) was close to the requirement. Thirty barrows (initial body weight: 16.37 ± 1.28 kg) were housed individually in metabolism crates and allotted to a randomized complete block design with 5 diets and 6 replicate pigs per diet. Feed was provided at 3.2 times the energy requirement for maintenance in 2 equal daily meals. Pigs had ad libitum access to water. After a 7-day adaptation period, fecal and urine samples were collected for 4 days to determine N balance. Data were analyzed using the MIXED procedure of SAS, and orthogonal-polynomial contrasts were used to determine linear effects of DL-Met and MHA-Ca levels and the effect of Met sources. Results indicated that pigs fed diets without supplementation of Met had reduced (P < 0.05) final BW, feed intake, and N intake compared with pigs fed diets supplemented with DL-Met or MHA-Ca, and no differences in final BW, feed intake, or N intake between pigs fed DL-Met or MHA-Ca were observed (Table 1). Fecal N increased (linear, P < 0.05) by supplementation of DL-Met to the diets, and urine N decreased (linear, P < 0.05) as MHA-Ca increased in the diet. Retention of N (g/day) increased (linear, P < 0.05) as DL-Met or MHA-Ca increased and N retention calculated as percent of intake increased (linear, P < 0.05) if MHA-Ca was added to the diet and tended to increase (linear, P < 0.10) if DL-Met was added to the diets. Retention of N calculated as percent of absorbed N also increased (linear, P ≤ 0.05) with increased Met in the diet regardless of source. However, retention of N measured as percent of intake was not different between DL-Met and MHA-Ca. In conclusion, pigs fed Met-deficient diets and supplemented with 65 parts of DL-Met or 100 parts of MHA-Ca on a product-to-product (wt/wt) basis did not have different N retention expressed as g per day, as percent of intake, or as percent of absorbed.","PeriodicalId":14895,"journal":{"name":"Journal of animal science","volume":"55 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144104594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ashlee Gibbons, Grant I Crawford, James Slightom, Haley E Larson
{"title":"191 Field validation of mob composite sampling protocol to estimate herd parasitism in cattle","authors":"Ashlee Gibbons, Grant I Crawford, James Slightom, Haley E Larson","doi":"10.1093/jas/skaf102.033","DOIUrl":"https://doi.org/10.1093/jas/skaf102.033","url":null,"abstract":"Cattle, when initially placed into the feedlot, frequently carry a significant internal parasite load, that if unaddressed, can result in weight loss, poor growth, and increased susceptibility to illness due to immune suppression. To combat these challenges, many feedlot animal health protocols include administering anthelmintic treatments immediately upon feedlot arrival. The detection of parasite loads and potential resistance to anthelmintic drugs typically involves fecal egg count protocols conducted using trained laboratory methods. However, these tests can be costly and may not be consistently used across operations. This study aimed to evaluate the efficacy of existing fecal sampling protocols against a modified mob composite sampling approach, intending to reduce cost and time required for results while encouraging more frequent sampling in commercial settings. A total of 920 fecal samples were collected from cattle (both calf-fed and yearling) in commercial feedlots across the midwestern United States. The cattle, originating from various sources (n=13), were either being sold at a sale barn or transported to feedlots or stocker units for finishing. Samples were collected from fresh fecal pats on the pen floor shortly after the arrival of the animals, with effective communication among feedlot managers and employees being crucial to ensure sampling occurred prior to anthelmintic treatments. Each sampling set corresponded to a pen of cattle (n=46), with pen sizes varying from 64 to 225 head, depending on the specific commercial operation. From each identified fecal pat, approximately 15 ml of material was collected from three different sites. This homogenized mixture of approximately 45 ml constituted one individual sample (IDV). The process was repeated with 20 distinct, fresh fecal pats within each pen, reflecting the industry-standard sampling protocol. The average of the 20 samples provided an eggs per gram (epg) value indicative of the parasite load within that cattle group (range 0 to 444 epg). For mob samples (n=92), 7.5 ml from ten individual samples were combined. Each pen produced two composite (MOB) samples, which were mixed by stirring in a smooth-sided cup using a tongue depressor. The averaged values from these two MOB samples yielded an epg representing the parasite load for that group. To evaluate the effectiveness of the MOB sampling method in producing values comparable to the IDV industry standard, Lin’s concordance correlation coefficient was utilized. The analysis revealed a 91.7% agreement in mean fecal egg counts between the MOB and IDV methods, with a 95% confidence interval ranging from 86.4% to 95.0%. These results suggest that the two-sample mob approach, utilizing a tongue depressor and smooth sided cup for mixing, may provide an acceptable method for assessing parasite loads in commercial feedlot operations, thus enhancing efficiency and cost-effectiveness.","PeriodicalId":14895,"journal":{"name":"Journal of animal science","volume":"25 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144104624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ester Vinyeta, Deepak E E Velayudhan, Pau Aymerich, Janet C Remus, Yueming Dersjant-Li
{"title":"PSII-14 Effect of a consensus bacterial 6-phytase variant (PhyG) on energy and amino acid digestibility and the impact on production performance in pigs","authors":"Ester Vinyeta, Deepak E E Velayudhan, Pau Aymerich, Janet C Remus, Yueming Dersjant-Li","doi":"10.1093/jas/skaf102.317","DOIUrl":"https://doi.org/10.1093/jas/skaf102.317","url":null,"abstract":"Phytase is commonly used in pig feed to improve the availability of phosphorus (P) and other minerals such as calcium (Ca) and sodium (Na). However, a phytase that can break down phytate quickly and completely in the early gut, can also reduce the anti-nutritional effect of phytate, resulting in an improved amino acid (AA) and energy digestibility. Recent studies demonstrated that a consensus bacterial 6-phytase variant (PhyG) consistently improved apparent ileal AA digestibility (meta-analysis of 8 datasets) and ileal energy digestibility in pigs (meta-analysis of 4 datasets). Application of digestible AA and energy matrix for phytase alone, or in combination with other enzymes, can reduce the feed cost and improve pig production benefits. This concept has been tested in 3 studies including one study in piglets and two studies from weaning to slaughter. In Exp. 1, three treatments were tested including a nutritionally adequate positive control (PC) and 2 PhyG-supplemented diets, reduced in digestible P, Ca, Na, net energy and digestible AA vs. PC, based on the contribution of PhyG added at 1,000 or 2,000 FTU/kg, respectively. The data showed that the application of full matrix could maintain or improve piglet performance and lead to up to 5.5% reduction in feed cost per kg body weight gain. In 2nd Exp., weaned pigs were randomized to 4 treatments and fed test diets in 5 phases, from weaning until market weight. Treatments included: a nutritionally sufficient positive control (PC) and 3 test diets with PhyG alone at low or high dose (phased dosing in different phases), or PhyG at low dose in combination with xylanase–β-glucanase (XB). All test diets were formulated with application of full energy and nutrient matrix. Overall growth performance (from weaning to slaughter) in all enzymes supplemented diets with full matrix application, was maintained or improved compared to PC, and there was a reduction in feed cost per kg body weight gain by up to 5.8%. In the third experiment, weaning pigs were randomly distributed to 3 treatments corresponding to a control diet, a PhyG treatment with diets supplemented with 1500 FTU/kg of PhyG, and a third treatment supplemented with the same PhyG dose and a multi-enzyme (Xylanase, β-glucanase, Amylase, and Protease). The enzyme supplemented diets were formulated with corresponding matrix values. Application of full energy and nutrient matrix for phytase alone or in combination with a multi-enzyme maintained the performance and carcass yield and quality of pigs from weaning to slaughter, leading to feed cost savings. In summary, the application of digestible AA and energy matrix, in addition to mineral matrix, can reduce feed cost and improve pig production benefits.","PeriodicalId":14895,"journal":{"name":"Journal of animal science","volume":"45 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144104625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ethan B Stas, Michael D Tokach, Joel M DeRouchey, Jason C Woodworth, Robert D Goodband, Jordan T Gebhardt
{"title":"PSIII-12 Evaluation of dietary acidifiers in low acid-binding capacity-4 diets on nursery pig performance and fecal dry matter","authors":"Ethan B Stas, Michael D Tokach, Joel M DeRouchey, Jason C Woodworth, Robert D Goodband, Jordan T Gebhardt","doi":"10.1093/jas/skaf102.330","DOIUrl":"https://doi.org/10.1093/jas/skaf102.330","url":null,"abstract":"A total of 300 pigs (initially 6.1 kg) were used to evaluate dietary acidifiers in low acid-binding capacity-4 (ABC-4) diets on nursery pig performance and fecal dry matter (DM). At weaning, pigs were randomly allotted to 1 of 6 dietary treatments. There were 5 pigs per pen and 10 replications per treatment. Pigs were fed experimental diets in two phases with phase 1 fed from d 0 to 10 post-weaning followed by phase 2 from d 10 to 24. Four diets were formulated to an ABC-4 level of 200 and 250 meq/kg in phase 1 and 2, respectively, by utilizing 4 different dietary acidifiers. Dietary acidifiers included 0.36% fumaric acid (Primary Products Ingredients Americas LLC, Decatur, IL), 0.87% Activate DA (Novus, St. Charles, MO), 1.01% KEM-GEST (Kemin, Des Moines, IA), and 0.85% ACID-AID (Alltech, Nicholasville, KY). Diet 5 did not include any dietary acidifiers which increased the ABC-4 level by 40 meq/kg in both phases. All diets contained 110 mg/kg of Zn provided by the trace mineral premix. Diet 6 was the same formulation as diet 5, but with the addition of 3,000 and 2,000 mg/kg of Zn from ZnO in phase 1 and 2, respectively. The addition of ZnO further increased the ABC-4 level of the diet by 87 and 54 meq/kg in phase 1 and 2, respectively. Following phase 2 experimental diets, all pigs were fed a common corn-soybean meal-based diet until study completion on d 38 post-weaning. There were no differences (P > 0.05) observed between pigs fed the four dietary acidifiers for the duration of the study. In phase 2 (d 10 to 24), the experimental period (d 0 to 24), and overall (d 0 to 38), pigs fed the low ABC-4 diets containing acidifiers had improved (P ≤ 0.020) G:F compared to pigs fed the 40 meq/kg higher ABC-4 diet (without acidifiers). In the experimental period (d 0 to 24), pigs fed the diet containing ZnO tended to have improved (P = 0.052) G:F compared to pigs not fed ZnO. In conclusion, using dietary acidifiers to achieve a low ABC-4 level improved feed efficiency, but there were no differences found between the acidifier sources used in this study. Most dietary acidifiers were included above their supplier-recommended inclusion rates to achieve the targeted low ABC–4 level and only decreased the ABC-4 by 40 meq/kg. Therefore, other aspects of low ABC-4 diet formulation such as low ABC-4 specialty protein sources, lactose sources, low Ca levels, a combination of acidifiers, or other methods must be considered when formulating to target low dietary ABC-4 levels.","PeriodicalId":14895,"journal":{"name":"Journal of animal science","volume":"236 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144104653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"PSV-18 Effects of oats fodder and quantitative feed restriction on egg and meat quality of native Bangladeshi geese","authors":"Umme Saleheen Ety, Md Sazedul Karim Sarker, Farhana Sharmin, Masud saleheen rana, Nushrat Nourin Lisa, Aminul Islam","doi":"10.1093/jas/skaf102.342","DOIUrl":"https://doi.org/10.1093/jas/skaf102.342","url":null,"abstract":"The present study was designed to assess the efficacy of Oats (Avena sativa) fodder as a supplementation along with concentrate feed for determining egg quality, meat color and texture, pH, drip loss, cooking loss, and water holding capacity (WHC) of Bangladeshi White and Gray geese varieties. A total of 60 adult geese from White and Grey varieties were reared in a semi-intensive production system in Bangladesh Livestock Research Institute, Savar. After 3 months of rearing, we collected freshly laid eggs for 4 weeks and 20 eggs /bird and 480 eggs for egg quality analysis. After that, those birds were slaughtered for meat quality analysis at 38 weeks of age. They were randomly divided into 4 groups with 3 replicates having 5 geese in each replication. Maize soya-based diet was considered as Basal diet and marked as T0- 200g concentrated feed, T1= 180 g Basal diet+ 138 g oats fodder, T2= 160 g Basal diet+ 276 g oats fodder, T3= 140 g Basal diet + 415 gm oats fodder daily. Egg quality was determined through the egg tester analyzer ultimate machine. A calibrated waterproof pH meter equipped with a spear tip probe was used to measure the pH values of the breast and thigh meat 24 hours of slaughter. Meat color was determined in triplicate using a Chromo meter CR-8, (3nh, China) and texture were determined using a texture analyzer (Tx-700, France). The data were statistically analyzed using SPSS software (23 version). Treatment had a remarkable effect on Albumen index, yolk CP % and breast meat cohesiveness value (p<.05). For egg quality parameter result showed that those traits were not significantly varied but tended to be higher in T2 group. Yolk and albumen CP% for T2 group was higher than other groups, but Breast meat cohesiveness tended to be lower in that group. However T2 group showed lower drip loss and cooking loss, higher WHC and also higher a* (redness) value but these parameters were not significantly affected by treatments. Supplementation of native Bangladeshi adult geese with 160 g concentrate feed+ 276 g oats fodder daily was found to be beneficial for improving egg and meat quality.","PeriodicalId":14895,"journal":{"name":"Journal of animal science","volume":"35 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144104654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hannah Miller, Chiron J Anderson, Stephan Schmitz-Esser, Aaron Ericsson, Amy L Petry
{"title":"28 Influence of corn based fibrous co-products on ileal mucosa gene expression and mucosal associated microbiome of growing pigs","authors":"Hannah Miller, Chiron J Anderson, Stephan Schmitz-Esser, Aaron Ericsson, Amy L Petry","doi":"10.1093/jas/skaf102.105","DOIUrl":"https://doi.org/10.1093/jas/skaf102.105","url":null,"abstract":"The microbiome located on the mucosal layer of the gastrointestinal tract is essential for nutrient exchange and host communication. Understanding the presence of the microbiome and its relationship to the host gene expression is essential to fully understand nutrient uptake and ingredient utilization. High inclusions of industrial corn co-products in grow-finish diets may affect gastrointestinal tissue function and alter microbial community structure. The objective of this study was to determine the influence of insoluble corn-based fibers (ICBF) on small intestine microbial communities and ileal transcriptome of growing pigs. Two replicates of 28 gilts (26.7 ± 2.5 kg BW; PIC800 x Camborough; N=56), were randomly assigned to one of 7 semi-synthetic diets. Dietary treatments included a control (CTL) diet with a formulated total dietary fiber (TDF) value of < 1% and 6 diets with an ICBF replacing 30% of the corn starch in CTL: dehulled degermed corn (DHDG; TDF = 0.89%), ground corn (COR; TDF = 3.80%), corn gluten meal (CGM; TDF = 4.36%), high protein dried distillers grains (HP; TDF = 7.00%), dried distillers grains (DDGS; TDF = 7.93%), and corn bran (BRN; TDF = 12.29%). Pigs were individually housed and limit-fed 2.4 times maintenance. On day 31 pigs were necropsied. Mucosal scrapings and tissue were collected from the ileum for 16S rRNA gene microbiota analysis and host differential expressed genes (DEG), respectively. Individual operational taxonomic units (OTU) were compared using Linear Discriminant Analysis Effect Size. Data for alpha diversity metrics were analyzed as a mixed model with replicate as random effect and treatment as fixed effect. Sixty of the top 100 OTUs differed across dietary treatments, mainly from the families Clostridiaceae, Enterobacteriaceae, and Streptococcaceae. There were no differences among observed species richness, Chao1, or Shannon alpha diversity indices. Simpson evenness tended to increase with increasing level of ICBF (P=0.09). Diets containing ICBF were compared to CTL for DEG. Protein-coding genes were selected based on a false discovery rate ≤0.05 and log2 fold change >|2|. Of the 23 genes that met these criteria, 14 were associated with intestinal integrity and cellular homeostasis. Differences were primarily identified in the high fiber diets: HP, DDGS, and BRN. Genes associated with stress response, CHAC1 and ATF5, were down regulated in HP compared to CTL (Q< 0.05). RND3 (RhoE) and WHRN are associated with regulating actin in the cytoskeleton and maintaining structure and function of the intestinal epithelium and were up regulated in DDGS relative to CTL (Q< 0.05). SERPINB5, TPPP3, and RFX2 were up regulated in BRN relative to CTL(Q< 0.05). These genes are associated with cellular structure and proliferation. In summation, these data imply corn co-products influence the small intestine microbiota and alter host gene expression in growing pigs.","PeriodicalId":14895,"journal":{"name":"Journal of animal science","volume":"55 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144104556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}