Journal of animal science最新文献

{"title":"Exploring the effects of dietary lysine and tryptophan on the social behavior of pigs.","authors":"Eleanor Hewett, Luis Zaragoza, Craig Lewis, Jos Houdijk, Andrea Wilson, Simon Turner","doi":"10.1093/jas/skaf030","DOIUrl":"10.1093/jas/skaf030","url":null,"abstract":"<p><p>Negative social behaviors between pigs can cause stress, which can compromise welfare. There has been significant interest in exploring the effect of diet on negative social behaviors and the wider social behavior repertoire of pigs. The aim of this study was to determine the effects of dietary Lysine (Lys) and Tryptophan (Trp) levels on the social behavior of commercially housed pigs. A total of 2,293 PIC Camborough barrows with a mean starting weight of 11.87 ± 1.35 kg were used in a randomized complete block design with a 2 × 3 factorial arrangement, with 16 replicates per treatment, of the following factors: 1) Standardized ileal digestible Lys levels: 100% Lys = diets with 100% PIC requirement at the midpoint of the growth phase (Lys 100) vs 80% Lys = diets with 80% PIC requirement at the midpoint of the growth phase (Lys 80); and 2) Trp to Lys ratio of 0.210, 0.185, or 0.160. Pigs were randomly allocated across the 6 treatments over 2 starting dates. Behavior and lesion data were collected. There was an effect of Lys (P = 0.032) on ear-biting behavior, with pigs on the Lys 80 treatments showing a higher level of ear-biting behavior. We also found an effect of the Lys score week interaction on the proportion of pens showing ear lesions (P < 0.001) and an effect of the Lys Trp interaction (P = 0.030) and the Lys score week interaction (P = 0.0104) on the proportion of pens showing severe ear lesions. In conclusion, the lysine content of feed can affect the social behavior of pigs, specifically ear biting, in commercial conditions.</p>","PeriodicalId":14895,"journal":{"name":"Journal of animal science","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11912881/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143255527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of ground herb-based and essential oil-based phytobiotics on the intestinal health and performance of nursery pigs challenged with F18+Escherichia coli.","authors":"Yesid Garavito-Duarte, Marcos Elias Duarte, Sung Woo Kim","doi":"10.1093/jas/skaf018","DOIUrl":"10.1093/jas/skaf018","url":null,"abstract":"<p><p>This study aimed to evaluate the efficacy of using ground herb-based phytobiotics and essential oil-based phytobiotics in pig diets on intestinal health and growth performance (GP) of nursery pigs challenged with F18+Escherichia coli. Forty nursery pigs (6.4 ± 0.1 kg) at 21 d of age were individually housed and assigned to 4 dietary treatments in a randomized complete block design, with body weight and sex as blocking factors. Basal diets were fed to pigs for 28 d in 3 phases. Treatments were negative control (NC): basal diet, non-challenged; positive control (PC): basal diet, challenged with F18+E. coli; HP: PC + 1% ground herb-based phytobiotics (Salcochek Pro, Ayurvet Limited, Kaushambi, India); EP: PC + 1% essential oil-based phytobiotics (Liq-biotic, Ayurvet Limited). The GP was recorded for each phase and fecal score (FS) was measured daily. On day 7 postweaning, the challenged groups were orally inoculated with F18+E. coli (2.0 × 1010 CFU), the NC treatment received a sterile saline solution. On day 28, pigs were euthanized to collect jejunal samples to evaluate intestinal health and relative abundance (RA) of jejunal mucosa-associated microbiota. Data were analyzed using the MIXED procedure on SAS 9.4. The PC increased (P < 0.05) the RA of Prevotellaceae, Lachnospiraceae, and Ruminococcaceae when compared to NC. The HP reduced (P < 0.05) the RA of Veillonellaceae, Prevotellaceae, and Lachnospiraceae when compared to PC. The EP tended to reduce the RA of Streptococcaceae (P = 0.073) and Corynebacteriaceae (P = 0.074) when compared to PC. The PC increased (P < 0.05) occludin and tended to increase (P = 0.096) toll-like receptor-4 (TLR4) when compared to NC. The PC decreased (P < 0.05) average daily gain and average daily feed intake when compared to NC in days 7 to 28. The PC increased FS (P < 0.05) compared to the HP and EP days 7 to 11. The HP and EP decreased (P < 0.05) FS when compared to PC during days 7 to 11 and days 7 to 18. In conclusion, F18+E. coli challenge disrupted the jejunal mucosa-associated microbiota, increased TLR4 expression and FS, and consequently reduced GP. Both HP and EP phytobiotics supported intestinal morphology during the challenge to F18+E. coli by supporting enterocyte maturation. The HP and EP treatments exhibited antimicrobial-like effects by altering the jejunal mucosa-associated microbiota and reduced FS during the first 2 weeks post-challenge. The HP treatment showed potential antioxidant effects.</p>","PeriodicalId":14895,"journal":{"name":"Journal of animal science","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11897891/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143065741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: Values shared by journals of learned societies, associations and scientific institutions in animal science.","authors":"I Ortigues-Marty, I Louveau, G Bee, J W Oltjen, P J Kononoff, J A A McArt, C Thomas, B D Fairchild, M Kogut, E Huff-Lonergan","doi":"10.1093/jas/skae379","DOIUrl":"10.1093/jas/skae379","url":null,"abstract":"","PeriodicalId":14895,"journal":{"name":"Journal of animal science","volume":"103 ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11879296/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143556952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative differences in rumen epithelium proteins and detection of lysine acetylation in lambs fed a low or high metabolizable energy diet.","authors":"Judith J Bond, Nicholas J Hudson, Karthik S Kamath, David Wheeler, Serey Woodgate, Victor H Oddy","doi":"10.1093/jas/skaf027","DOIUrl":"10.1093/jas/skaf027","url":null,"abstract":"<p><p>Thirty-six Merino wethers (10-mo-old) were fed ad libitum for 30 d 2 diets;1) low metabolizable energy diet (LME; 30% lucerne: 70% cereal chaff) and 2) high ME diet (HME; 40% rolled barley grain: 50% lucerne: 10% cereal chaff). Effects of diet on dry matter intake (DMI), ME intake (MEI), liveweight (LWT), average daily gain (ADG), carcass lean or fat gain, liver and empty rumen weight, and plasma metabolites were analyzed. A membrane-enriched protein fraction of rumen epithelium (RE) isolated enzymatically from whole depth rumen wall was quantified for each sheep using tandem mass tag mass spectrometry (TMT-MS). The presence or absence of acetylation of lysine residues on identified proteins was counted and the position of the lysine acetylation was recorded. In lambs fed the HME diet, DMI (P < 0.001), MEI (P < 0.001), ADG (P < 0.001), fat (P < 0.001), and lean gain (P < 0.001), as well as liver (P < 0.001) and empty rumen (P < 0.009) weight were greater than those fed the LME diet. Plasma glucose (P < 0.001) and βhydroxybutyrate (P < 0.001) at 3 and 5 h after feeding was greater in HME diet than in the LME-fed lambs. Changes in RE protein abundance in the LME versus HME-fed lambs were associated with metabolism in the peroxisome, protein processing in the endoplasmic reticulum (ER), valine, leucine and isoleucine degradation, and carbon metabolism. Acetylation of lysine was detected in enzymes involved in glycolysis, tricarboxylic acid (TCA) cycle, and fatty acid (FA) metabolism. Quantitative differences in the abundance of RE proteins that carry out intracellular processes of energy expenditure were associated with the concentration of ME (MJ/ kg DM) in the diet of growing lambs. The detection of lysine acetylation sites suggests a difference in the ME of the diet regulates enzymatic activity in central metabolic pathways in the RE cells.</p>","PeriodicalId":14895,"journal":{"name":"Journal of animal science","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11912824/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143188859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Examination of the role of the rumen in zinc metabolism.","authors":"Olivia N Genther-Schroeder, Dathan T Smerchek, Gregory B Penner, Stephanie L Hansen","doi":"10.1093/jas/skaf052","DOIUrl":"10.1093/jas/skaf052","url":null,"abstract":"<p><p>Three experiments were conducted to characterize Zn appearance and disappearance in the rumen, rumen epithelial Zn importers and exporters, the directionality of Zn movement, and the effect of supplemental Zn on rumen epithelial structure. In experiments 1 and 2, ruminally fistulated steers (n = 4) weighing 736 ± 23.3 kg were used in a crossover experiment with 2 dietary Zn concentrations (ZINC; 0 [CON] or 120 mg supplemental Zn/kg DM [Zn120], from ZnSO4) in a 45% forage, 55% concentrate diet (experiment 1) or a 92.5% concentrate and 7.5% forage diet (experiment 2) to assess Zn appearance and disappearance in the rumen using the washed reticulo-rumen technique (WRR). Experiment 3 used 20 individually housed Hampshire crossbred wether lambs (35.1 ± 4.57 kg) fed for 60-d to evaluate the impact of dietary grain and Zn concentrations on ruminal epithelium Zn concentration, Zn transporters, and proteins integral to epithelial integrity. This experiment was a 2 × 2 factorial, with 2 diet types (DIET; 45% forage and 55% concentrate [HF] or 7.5% forage and 92.5% concentrate [HG]), and 2 supplemental Zn treatments (ZINC; 0 mg [CON] or 120 mg [ZN] supplemental Zn/kg diet DM) from ZnSO4. In experiments 1 and 2, ZINC did not affect the rate of disappearance (k) or plateau (B) digestive model parameters for buffer Zn (P ≥ 0.34) but ruminal Zn disappearance was numerically greater in experiment 2. In experiment 3, there was a DIET × ZINC effect (P = 0.05) where HG + ZN had the greatest rumen epithelial Zn, and HF + ZN had the least. There was an interaction between DIET and ZINC on papillae length (P = 0.05), where papillae length was shorter in HF than HG, and within HG, ZN animals had shorter papillae than CON (P = 0.01). A DIET × ZINC effect was noted for ZnT1 RNAscope score (P = 0.01) within the stratum spinosum layer where HG + CON wethers had greater mean ZnT1 RNAscope score than other treatments (P ≤ 0.006). Ruminal Claudin-7 protein expression was lesser in HF (P = 0.01) and tended to be lesser in ZN (P = 0.09). Overall, these experiments highlight the dynamic nature of ruminal Zn metabolism and are supporting evidence for the relationship between ruminal health and dietary grain and Zn concentrations.</p>","PeriodicalId":14895,"journal":{"name":"Journal of animal science","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11929949/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143468056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of increasing dietary zinc oxide levels on the hepatic mitochondrial energy metabolism, oxidative balance, and antioxidant system in weaned piglets.","authors":"Geneviève Villeneuve, Caroline Roy, Karine Deschêne, Jean-Jacques Matte, Jérôme Lapointe, Danyel Bueno Dalto","doi":"10.1093/jas/skaf031","DOIUrl":"10.1093/jas/skaf031","url":null,"abstract":"<p><p>This study compared different levels of dietary zinc oxide (ZnO) on mitochondria ATP synthesis and the cellular and systemic redox balance in weaned pigs. One hundred twenty weaned piglets (7.96 ± 1.17 kg, 21 days of age) were randomly allocated in one of three diets containing different Zn levels (as ZnO); 100 (100Zn), 1,000 (1000Zn) and 3,000 mg/kg (3000Zn), and were slaughtered at day 21, 23, 35 or 42 for the collection of blood and liver samples. Dietary copper (Cu) levels were constant at 131 mg/kg. Hepatic mitochondrial concentrations of Zn and Cu, hepatic mitochondria respiration, antioxidant response, and the hepatic expression of related genes were analyzed. Piglets fed 3000Zn had the highest Zn (P < 0.01) and the lowest Cu concentrations (P = 0.01) in hepatic mitochondria. The hepatic oxygen consumption rate for maximal respiration was the highest in 3000Zn piglets (P < 0.05), whereas hepatic cytochrome c oxidase activity tended to be lower (P = 0.06) and intracellular ATP concentrations were the lowest (P = 0.01). Mitochondrial Cu/Zn superoxide dismutase (Cu/Zn-SOD) activity was the lowest (P < 0.01) and glutathione peroxidase (GPx) activity tended to be the lowest (P = 0.07) for 3000Zn piglets. The mRNA expression of the antioxidant-related genes copper chaperone for superoxide dismutase (CCS) was highest (P = 0.01) in 3000Zn piglets and tended to be the highest (P = 0.07) for glutathione synthetase (GSS). Plasma carbonyls concentrations (P = 0.02) and GPx activity (P = 0.01) were the highest while SOD activity was not impacted (P = 0.55) in 3000Zn piglets. In conclusion, supplementing 3000 mg Zn/kg had detrimental impacts on mitochondria Zn and Cu homeostasis, resulting in a dysfunctional mitochondria respiratory chain and disturbed antioxidant response in postweaning piglets.</p>","PeriodicalId":14895,"journal":{"name":"Journal of animal science","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11929951/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143255523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of soybean gums and soybean soapstocks on weanling pig growth performance, fecal dry matter, and apparent total tract digestibility of dry matter.","authors":"Katelyn N Gaffield, Robert D Goodband, Joel M DeRouchey, Mike D Tokach, Jason C Woodworth, Gordon Denny, Carmen Slipher, Hari B Krishnan, Jordan T Gebhardt","doi":"10.1093/jas/skaf051","DOIUrl":"10.1093/jas/skaf051","url":null,"abstract":"<p><p>Depending on the processing plant, gums and soapstocks may be added back to soybean meal. There is potential for these by-products to serve as an affordable energy source for swine due to their residual oil content. A total of 350 pigs (Line 241 × 600, DNA; initially 5.3 ± 0.02 kg) were weaned at approximately 19 d of age and used in a 42-d experiment. At weaning, pigs were randomly assigned to pens and allotted to 1 of the 5 treatments. There were 5 pigs per pen and 14 pens per treatment. Diets were fed in 3 phases: phase 1 from weaning to day 11, phase 2 from days 11 to 23, and phase 3 from days 23 to 42. Treatments included a control diet containing soybean meal with no added soybean by-products. Two additional diets contained gums or soapstocks at 4% of the soybean meal level in the diet. Another treatment diet contained soybean meal with 2% added soybean gums and 2% added soybean soapstocks. Finally, a negative control contained 4% less soybean meal with no added by-products to have equal protein from soybean meal to diets with added gums or soapstocks. Feces were collected on days 11 and 23 from 3 pigs per pen to determine fecal dry matter (DM). Fecal samples taken on day 23 were used to determine the apparent total tract digestibility (ATTD) of DM. From days 0 to 11 (phase 1) and days 11 to 23 (phase 2), there was no evidence of differences (P > 0.10) for any growth response criteria. From days 23 to 42 (phase 3), pigs fed soybean meal with added gums had increased (P = 0.05) average daily gain (ADG). However, there was no evidence for differences (P > 0.10) in average daily feed intake (ADFI) or gain-to-feed ratio (G:F). For the overall experimental period (days 0 to 42), there was no evidence of differences (P > 0.10) due to dietary treatment for ADG, ADFI, or G:F. Fecal DM was approximately 19% on both days 11 and 23 and was not affected (P > 0.10) by treatment. There was an interaction (P = 0.019) between soybean gum and soapstocks for the ATTD of DM. When adding 4% gums to the diet, there was an improvement in the ATTD of DM; however, there was no evidence of differences when adding 4% soapstocks or 2% gums and 2% soapstocks to soybean meal. These data suggest adding soybean processing by-products to soybean meal has minimal effects on nursery pig growth performance. However, there is a potential for improved ADG in the late nursery period when soybean meal containing added gums is included in the diet.</p>","PeriodicalId":14895,"journal":{"name":"Journal of animal science","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11956680/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143468114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polyamines protect porcine sperm from lipopolysaccharide-induced mitochondrial dysfunction and apoptosis via casein kinase 2 activation.","authors":"Rongnan Li, Xiaodong Wu, Jia Cheng, Zhendong Zhu, Ming Guo, Guochao Hou, Tianjiao Li, Yi Zheng, Haidong Ma, Hongzhao Lu, Xiaoxu Chen, Tao Zhang, Wenxian Zeng","doi":"10.1093/jas/skae383","DOIUrl":"10.1093/jas/skae383","url":null,"abstract":"<p><p>Bacterial contamination is an inevitable issue during the processing of semen preservation in pigs. As a prototypical endotoxin from Gram-negative bacteria in semen, lipopolysaccharide (LPS) undermines sperm function during liquid preservation. Spermine and spermidine could protect cells against LPS-induced injury, and the content of spermine and spermidine in seminal plasma is positively correlated with sperm quality. Thus, the present study aimed to clarify whether addition of spermine or spermidine is beneficial to porcine semen preservation and able to prevent LPS-induced sperm damage. The supplementation of spermine and spermidine in the diluent resulted in higher sperm motility, viability, acrosome integrity, and mitochondrial membrane potential (ΔΨm) after preservation in vitro at 17 °C for 7 d (P < 0.05). LPS-induced sperm quality deterioration, ΔΨm decline, cellular adenosine-triphosphate depletion, mitochondrial ultrastructure abnormality, mitochondrial permeability transition pore opening, phosphatidylserine (PS) translocation, and caspase-3 activation (P < 0.05). Interestingly, spermine and spermidine alleviated the LPS-induced changes of the aforementioned parameters and mitigated the decrease in the microtubule-associated protein light chain 3-II (LC3-II) to LC3-I ratio. Meanwhile, the α and β subunits of casein kinase 2 (CK2) were detected at the connecting piece and the tail. Significantly, addition of 4,5,6,7-tetrabromobenzotriazole, a specific CK2 inhibitor, counteracted the beneficial effects of spermine and spermidine on sperm quality, mitochondrial activity, and apoptosis. Together, these results suggest that spermine and spermidine improve sperm quality and the efficiency of liquid preservation of porcine semen. Furthermore, spermine and spermidine alleviate LPS-induced sperm mitochondrial dysfunction and apoptosis in a CK2-dependent manner.</p>","PeriodicalId":14895,"journal":{"name":"Journal of animal science","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11773192/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142864252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lactobacillus and Saccharomyces fermentation products impact performance and the fecal microbiome in weanling pigs inoculated with enterotoxigenic Escherichia coli.","authors":"Tanya Cherrington, David Jordan, John Pluske, Josie Mansfield, Kittitat Lugsomya, Stuart Wilkinson, David Cadogan, Sam Abraham, Mark O'Dea","doi":"10.1093/jas/skae394","DOIUrl":"10.1093/jas/skae394","url":null,"abstract":"<p><strong>Background: </strong>Enterotoxigenic F4 Escherichia coli (F4-ETEC) pose an economic threat to the swine industry through reduced growth, increased mortality and morbidity, and increased costs associated with treatment. Prevention and treatment of F4-ETEC often rely on antimicrobials; however, due to the threat of antimicrobial resistance, antimicrobial use is being minimized, and hence alternative control methods are needed. This study investigated the effects of postbiotics in the form of Lactobacillus acidophilus fermentation products (LFP) and Saccharomyces cerevisiae fermentation products (SFP), on pigs challenged with an F4 ETEC strain. Eighty pigs were selected based on a prescreening F4-ETEC susceptibility test. The animals were divided into 5 treatments each with 4 replicate pens. Pigs were assigned to 5 different diets: a control diet (CON); CON diet with 3,000 ppm ZnO (ZnO); CON diet with 2,000 ppm LFP (LFP); CON diet with 2,000 ppm SFP (SFP); CON diet with both 2,000 ppm LFP and 2,000 ppm SFP (LAS). Pigs were inoculated per os with F4-ETEC twice, on day 0 and day 1 of the experiment.</p><p><strong>Results: </strong>No significant differences in fecal consistency scores or fecal F4-ETEC concentration in pigs supplemented with LFP and/or SFP were detected. An increased diversity and abundance of Lactobacillaceae in the fecal microbiome of pigs supplemented with LFP were detected, as well as an increased final liveweight of pigs supplemented with LFP and/or SFP.</p><p><strong>Conclusion: </strong>This study demonstrated that the fecal microbiome is modified in F4-ETEC-challenged pigs supplemented with the combination of LFP and SFP, with these modifications previously associated with increased growth performance and health status in young pigs. Pigs receiving this combination of postbiotics also demonstrated an increased final liveweight, indicating that management of ETEC-associated performance loss may not require the complete removal of ETEC from a production system.</p>","PeriodicalId":14895,"journal":{"name":"Journal of animal science","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11842899/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143005794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seminal plasma metabolomics and sperm lipidomics profiles of bull semen with different total progressive motile sperm count.","authors":"Fanglin Pang, Yuke Sheng, LiuTao Gao, Hossam E Rushdi, Juan J Loor, QuanZhao Tian, Shenhe Liu","doi":"10.1093/jas/skaf012","DOIUrl":"10.1093/jas/skaf012","url":null,"abstract":"<p><p>Total progressive motile sperm count (TPMSC) is a reliable index of fecundity evaluation of bull semen. It is an important determinant of frozen semen yield and conception rate of females artificially inseminated. Seminal plasma metabolites and sperm lipids are closely related to sperm survival and motility, but their relationship with TPMSC is not well known. In the present study, Simmental bulls with higher (H, n = 6) or lower (L, n = 6) TPMSC (P < 0.01) were selected from a cohort of 100 animals aged 2 to 5 yr based on semen quality. Analysis of semen quality and biochemical markers of seminal plasma revealed that H bulls had greater ejaculate volume (P < 0.05), sperm motility, plasma membrane integrity rate (P < 0.01), seminal plasma neutral α-glucosidase (P < 0.05), alkaline phosphatase, acid phosphatase, cortisol and phosphatidylcholine (P < 0.01), and lower sperm malformation rate (P < 0.05) and reactive oxygen species (P < 0.01). Semen metabolites and sperm liposome profiles of H and L groups were compared using LC-MS/MS analysis. A total of 120 differentially abundant metabolites (VIP > 1; P < 0.05) and 59 differentially abundant lipids (VIP > 1; P < 0.05) were identified between H and L groups. Oxidative stress, sperm motility, and sperm plasma membrane integrity were among the enriched biological pathways. Cyclic ADP-ribose (cADPR), up-regulated in H bulls, is associated with energy for sperm motility and maintenance of membrane stability. Thymidineglycol (Tg), levanbiose, thymidine (Thd), and CE (3M5) were down-regulated in H bulls and may have negatively affected sperm motility. Correlation analyses revealed that TPMSC and sperm motility were significantly positively correlated with cADPR, while Tg, Levanbiose, Thd, and CE (3M5) were significantly negatively correlated with TPMSC and sperm motility. Thus, we speculate that these molecules may be exploited as potential biomarkers for non-invasive evaluation of TPMSC in bull semen.</p>","PeriodicalId":14895,"journal":{"name":"Journal of animal science","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11914886/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143065744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}