Journal of Advanced Pharmaceutical Technology & Research最新文献_第9页

The effect of red fruit oil (Pandanus conoideus Lamk.) emulgel on angiogenesis and collagen density in incisive wound healing in mice (Mus musculus) 红果油乳状液对小鼠切口愈合血管生成及胶原密度的影响

Journal of Advanced Pharmaceutical Technology & Research Pub Date : 2023-01-01 DOI: 10.4103/japtr.japtr_67_23

FidiaRizkiah Inayatilah, RiaRamadhani Dwi Atmaja, VenyKhairani Annadiyah, Maulida Thoyyibah, Siti Maimunah, Burhan Ma'arif, Roihatul Mutiah, IMade Budi, Muhammad Amiruddin

{"title":"The effect of red fruit oil (Pandanus conoideus Lamk.) emulgel on angiogenesis and collagen density in incisive wound healing in mice (Mus musculus)","authors":"FidiaRizkiah Inayatilah, RiaRamadhani Dwi Atmaja, VenyKhairani Annadiyah, Maulida Thoyyibah, Siti Maimunah, Burhan Ma'arif, Roihatul Mutiah, IMade Budi, Muhammad Amiruddin","doi":"10.4103/japtr.japtr_67_23","DOIUrl":"https://doi.org/10.4103/japtr.japtr_67_23","url":null,"abstract":"Pandanus conoideus Lamk. or commonly known as red fruit oil (RFO) can be used to accelerate wound healing because it contains tocopherols, carotenoids, oleic acid, linoleic acid, and linolenic acid. The RFO in this study was formulated in the form of an emulgel because it has the most convenient and effective drug delivery system. The aims of this study were to determine the activity of RFO emulgel on increasing the amount of angiogenesis and collagen density in incised wound healing and to determine the optimal dose of RFO emulgel to increase the amount of angiogenesis and collagen density in incised wound healing. This was a true experimental study with a posttest only control group design that included five treatment groups: a positive control group (10% povidone-iodine), a negative control (gel base), and three groups that varied the concentration of RFO emulgel used at 5%, 10%, and 15%. Parameters observed were the amount of angiogenesis using Image Raster software and the percentage of areas of collagen density using ImageJ software. The data were analyzed using a one-way ANOVA test and continued with the least significant difference test. The results of this study showed that RFO emulgel was able to increase the amount of angiogenesis and collagen density in the wound healing process with P = 0.000. An increase in the amount of angiogenesis and collagen density occurred in mice treated with RFO compared to the positive and negative control groups. It can be concluded that RFO emulgel has activity toward increasing the amount of angiogenesis and collagen density in the wound healing of mice incisions. The optimal dose concentration of RFO emulgel for increasing the amount of angiogenesis and collagen density in incision wound healing was shown in RFO emulgel with a concentration of 15%.","PeriodicalId":14877,"journal":{"name":"Journal of Advanced Pharmaceutical Technology & Research","volume":"83 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134887760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study of hesperetin effect on modulating transcription levels of MLH1 and MSH2 genes in SKBR3 breast cancer cell line 橙皮苷对SKBR3乳腺癌细胞系MLH1和MSH2基因转录水平调控的研究

Journal of Advanced Pharmaceutical Technology & Research Pub Date : 2023-01-01 DOI: 10.4103/japtr.japtr_278_23

Esmaeil Babaei, NaserHameed Saleh, AhmedSalim Kadhim Al-Khafaji

{"title":"Study of hesperetin effect on modulating transcription levels of MLH1 and MSH2 genes in SKBR3 breast cancer cell line","authors":"Esmaeil Babaei, NaserHameed Saleh, AhmedSalim Kadhim Al-Khafaji","doi":"10.4103/japtr.japtr_278_23","DOIUrl":"https://doi.org/10.4103/japtr.japtr_278_23","url":null,"abstract":"Hesperetin (HSP), a flavonoid, has been validated to modify gene expression and function as an epigenetic agent to stop the development of breast carcinoma cells. HSP was investigated in this research to evaluate the expression of the MLH1 and MSH2 genes in cancerous breast cell lines (SKBR3) and healthy cell lines (MCF-11A) after exposure to different dosages (200, 400, and 600 μM/mL) of HSP. After 48 h of exposure, SKBR3's half-maximal inhibitory concentration was 289.6 μM/mL and MCF-10A's was 855.4 μM/mL. The research found that increasing HSP concentrations were closely correlated with an increase in MLH1 gene levels in the SKBR3 cell line, as shown by median and percentile values. HSP therapy caused the MLH1 gene expression to substantially vary in different groups, and in the SKBR3 cell line, MSH2 gene expressions were elevated in a dose-escalating manner. Moreover, HSP also raised the number of apoptotic cells, with the fraction of apoptotic cells escalating substantially at doses of 400 and 600 μM/mL. The outcomes suggested that HSP has the potential to be utilized as a therapeutic intervention for breast cancer, as it can induce apoptosis and reduce cell viability.","PeriodicalId":14877,"journal":{"name":"Journal of Advanced Pharmaceutical Technology & Research","volume":"75 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134887911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design of experiments: A design to improve pharmaceutical research. 实验设计：改进药物研究的设计。

IF 1.4

Journal of Advanced Pharmaceutical Technology & Research Pub Date : 2023-01-01 Epub Date: 2023-01-20 DOI: 10.4103/japtr.japtr_690_22

Bhaswat S Chakraborty

引用次数: 0

Investigations of chemical compositions and antioxidative potential of essential oils isolated from the leaves of two Garcinia species. 从两种藤黄属植物叶片中分离的精油的化学成分和抗氧化潜力的研究。

IF 1.4

Journal of Advanced Pharmaceutical Technology & Research Pub Date : 2023-01-01 Epub Date: 2023-01-20 DOI: 10.4103/japtr.japtr_570_22

Shanthirasekaram Kokilananthan, Vajira P Bulugahapitiya, Harshi Manawadu, Chinthaka Sanath Gangabadage

{"title":"Investigations of chemical compositions and antioxidative potential of essential oils isolated from the leaves of two Garcinia species.","authors":"Shanthirasekaram Kokilananthan, Vajira P Bulugahapitiya, Harshi Manawadu, Chinthaka Sanath Gangabadage","doi":"10.4103/japtr.japtr_570_22","DOIUrl":"10.4103/japtr.japtr_570_22","url":null,"abstract":"Garcinia quaesita and Garcinia zeylanica are Sri Lankan endemic plants with significant therapeutic potential and numerous health-care applications. Despite this, there are no adequate literatures reported on the chemical compositions (CCs) and antioxidative potential (AP) of leaves' essential oils (EOs). The purpose of this study was to extract EOs from the leaves and investigate the CCs and AP of the extracted EOs. The hydro-distillation technique was used to extract the EOs, and the CCs of the EOs were identified through gas chromatography-mass spectrometry analysis. Only those compounds that had a matching value of more than 90% were taken into consideration, and the AP of the extracted EOs was determined using the ferric-reducing antioxidant power (FRAP) assay. Hydro-distillation process yielded EOs in the same quantity, 0.12% (v/w) on a fresh weight basis for two varieties. About 33 CCs that were found in the extracted EOs were mainly sesquiterpenes. The most prevalent substances in the EOs were copaene (19.39%), caryophyllene (12.94%), alloaromadendrene (12.12%), α-humulene (11.24%), and α-cubebene (9.38%). It is interesting to note that copaene and alloaromadendrene were only found in G. quaesita, whereas α-cubebene was only found in G. zeylanica. Caryophyllene and α-humulene were identified in both EOs at different concentrations. The EO from G. quaesita showed high AP, presenting FRAP values 274.74 ± 1.32 μL Trolox Eq/L. This study is recognized as being the first to examine the CCs and AP of EOs, and the results may inspire the creation of new uses and high-value leaf products.","PeriodicalId":14877,"journal":{"name":"Journal of Advanced Pharmaceutical Technology & Research","volume":"14 1","pages":"12-17"},"PeriodicalIF":1.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/79/af/JAPTR-14-12.PMC10026321.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9166594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Erratum: Formulation and characterization of orodispersible tablet of glimepiride. 勘误表：格列美脲口服分散片的配方和特性。

IF 1.4

Journal of Advanced Pharmaceutical Technology & Research Pub Date : 2023-01-01 Epub Date: 2023-01-20 DOI: 10.4103/2231-4040.368254

引用次数: 1

In silico identification of natural compounds from virgin coconut oil as potential ligand peroxisome proliferator-activated receptor-gamma as preventive food leads against colitis: Is it really work? 在计算机上鉴定来自初榨椰子油的天然化合物作为潜在的配体过氧化物酶体增殖物激活受体γ作为预防结肠炎的食物：它真的有效吗？

IF 1.4

Journal of Advanced Pharmaceutical Technology & Research Pub Date : 2023-01-01 Epub Date: 2023-01-20 DOI: 10.4103/japtr.japtr_505_22

Ni Made Rika Trismayanti, Kusworini, Handayani Dian

{"title":"In silico identification of natural compounds from virgin coconut oil as potential ligand peroxisome proliferator-activated receptor-gamma as preventive food leads against colitis: Is it really work?","authors":"Ni Made Rika Trismayanti, Kusworini, Handayani Dian","doi":"10.4103/japtr.japtr_505_22","DOIUrl":"10.4103/japtr.japtr_505_22","url":null,"abstract":"Ulcerative colitis (UC) is an inflammation of the large intestine characterized by diarrhea with blood. UC has a more extensive manifestation in children. Current therapy has not given satisfactory results. This is the basis for the need for preventive therapy to reduce the morbidity and mortality of UC in children. Virgin coconut oil (VCO) is a viable dietary supplement option due to its ability to act as a peroxisome proliferator-activated receptor (PPAR) ligand, inhibiting the release of pro-inflammatory cytokines. The aim of this study was to determine natural compounds from VCO that have the potential to prevent colitis using a docking-based virtual screening approach. Quantitative structure-activity relationship analysis was used to find out how similar the input compounds and the database were. Docking is done using AutoDockTools 1.5.6. The algorithm used is the Lamarckian Genetic Algorithm (4.2). PPAR-gamma (PPAR-γ) was used as the target protein in a complex with rosiglitazone (ID PDB: 7AWC). PyMol 2.5.1 was used to prepare and visualize three-dimensional data, and the amino acid interactions were visualized using Discovery Studio 2021 Clients. It was found that linoleic acid and oleic acid in VCO have anti-inflammatory effects with predictive values of 0.73 and 0.614, respectively, and that they stop tumor necrosis factor (TNF) expression with predictive values of 0.751 and 0.724. The result of molecular docking showed that the VCO compound was able to interact with the same residue as the control. VCO reduces inflammation by acting as a PPAR-γ and TNF expression inhibitor.","PeriodicalId":14877,"journal":{"name":"Journal of Advanced Pharmaceutical Technology & Research","volume":"14 1","pages":"39-45"},"PeriodicalIF":1.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/e0/0c/JAPTR-14-39.PMC10026328.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9166595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chromolaena odorata Linn leaf extract – Geothermal versus nongeothermal: Phytochemical, antioxidant, and cytotoxicity screenings 臭草叶提取物-地热与非地热:植物化学、抗氧化和细胞毒性筛选

Journal of Advanced Pharmaceutical Technology & Research Pub Date : 2023-01-01 DOI: 10.4103/japtr.japtr_286_23

Azzaki Abubakar, Hanifah Yusuf, Maimun Syukri, Rosnani Nasution, Rinaldi Idroes

{"title":"Chromolaena odorata Linn leaf extract – Geothermal versus nongeothermal: Phytochemical, antioxidant, and cytotoxicity screenings","authors":"Azzaki Abubakar, Hanifah Yusuf, Maimun Syukri, Rosnani Nasution, Rinaldi Idroes","doi":"10.4103/japtr.japtr_286_23","DOIUrl":"https://doi.org/10.4103/japtr.japtr_286_23","url":null,"abstract":"Chromolaena odorata Linn, a popular yet underutilized ethnomedicinal plant, is hypothesized to possess higher bioactive phytoconstituents when it grows in geothermal areas. In this study, the comparison of ethanolic extract from geothermal and nongeothermal C. odorata leaves was carried out based on the phytochemical profile, antioxidant activity, and cytotoxicity. The leaf extracts were produced from a maceration using ethanol 96%, where the products were identified using reagents and gas chromatography–mass spectrometry (GC-MS). Antioxidant activities of both samples were measured based on their 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activities. Cytotoxicity was determined by brine shrimp lethality test using Artemia salina. Phenols were found to be more abundant in geothermal sample based on the qualitative screening and GC-MS analysis (i.e. higher relative abundance of phytol – 3.97%). DPPH antioxidant was higher in geothermal sample than in nongeothermal sample (median inhibitory concentration =13.04 ± 3.35 mg/L vs. 41.09 ± 4.13 mg/L, respectively). Geothermal sample was noncytotoxic (median lethal concentration [LC50] =2139.30 mg/L), whereas the nongeothermal sample had low cytotoxicity (LC50 = 491.48 mg/L). Taken altogether, geothermal C. odorata leaves contain higher bioactive compounds with potent antioxidant activities.","PeriodicalId":14877,"journal":{"name":"Journal of Advanced Pharmaceutical Technology & Research","volume":"159 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134887910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Viability of human dental pulp stem cells: The potential of L-arginine-based culture media 人牙髓干细胞的生存能力:l -精氨酸基培养基的潜力

Journal of Advanced Pharmaceutical Technology & Research Pub Date : 2023-01-01 DOI: 10.4103/japtr.japtr_719_22

Anggraini Margono, SammyHenry Lay, DiniAsrianti Bagio, Indah Julianto

{"title":"Viability of human dental pulp stem cells: The potential of L-arginine-based culture media","authors":"Anggraini Margono, SammyHenry Lay, DiniAsrianti Bagio, Indah Julianto","doi":"10.4103/japtr.japtr_719_22","DOIUrl":"https://doi.org/10.4103/japtr.japtr_719_22","url":null,"abstract":"Dental pulp is built by proteins that have various roles in the biological process of pulp, such as structural protein, regulation protein, and catalytic protein. L-arginine, an amino acid and one of the building blocks of proteins, regulates pro-inflammatory and anti-inflammatory activity. Therefore, L-arginine-based culture has potential to promote dental pulp regeneration. This study aimed to investigate the potential of L-arginine-based culture in improving the viability of human dental pulp stem cells (hDPSCs). We evaluated the viability of hDPSCs in culture media supplemented with different concentrations of L-arginine amino acid (250, 300, 350, and 400 µmol/L) and Dulbecco’s Modified Eagle Medium plus fetal bovine serum 10% (control) using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay after 24-h incubation time. Statistical analysis was conducted using a one-way analysis of variance and post hoc least significant difference test. In qualitative analysis, the 4´, 6-diamidino-2-phenylindole staining method was used. The evaluation has shown a significant result when 250, 300, and 350 μmol/L concentration of L-arginine amino acid culture media compared with control, and 400 μmol/L has the best result and was not significantly different with control toward viability of hDPSCs.","PeriodicalId":14877,"journal":{"name":"Journal of Advanced Pharmaceutical Technology & Research","volume":"265 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134888024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic polymorphisms associated with sepsis incidence, severity, and outcomes among neonates: A mini-review 新生儿中与脓毒症发生率、严重程度和结局相关的遗传多态性:一项小型综述

Journal of Advanced Pharmaceutical Technology & Research Pub Date : 2023-01-01 DOI: 10.4103/japtr.japtr_332_23

Darnifayanti Darnifayanti, Muslim Akmal, Syahrun Nur, Sulaiman Yusuf

{"title":"Genetic polymorphisms associated with sepsis incidence, severity, and outcomes among neonates: A mini-review","authors":"Darnifayanti Darnifayanti, Muslim Akmal, Syahrun Nur, Sulaiman Yusuf","doi":"10.4103/japtr.japtr_332_23","DOIUrl":"https://doi.org/10.4103/japtr.japtr_332_23","url":null,"abstract":"Genetic variation remains a topic of great interest due to its potential as a risk factor for various diseases. Interactions between genes contribute to diverse phenotypes in response to factors such as infection. The impact of genetic background on susceptibility and clinical outcomes, particularly in neonatal sepsis, has gained recognition. The variability in sepsis susceptibility and outcomes can be attributed to the genetic diversity in coding regions and regulatory elements of genes related to innate immune response. Recent advances in genomics and technology have shed light on genetic polymorphisms among humans, often represented by single-nucleotide polymorphisms (SNPs). These SNPs encode proteins crucial for recognizing and responding to pathogenic bacteria, including Toll-like receptor 4, CD14, tumor necrosis factor-alpha, as well as interleukin-1-10. This literature review specifically discusses the involvement of genetic polymorphism during the pathogenesis stage of sepsis, with an emphasis on previous research findings in neonatal sepsis cases, aiming to discuss the implications of polymorphism in sepsis susceptibility and outcomes.","PeriodicalId":14877,"journal":{"name":"Journal of Advanced Pharmaceutical Technology & Research","volume":"72 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134888031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of additional sucrose on the value of tensile strength and elongation of chicken legs gelatin film. 添加蔗糖对鸡腿明胶薄膜拉伸强度和伸长率的影响。

IF 1.4

Journal of Advanced Pharmaceutical Technology & Research Pub Date : 2023-01-01 Epub Date: 2023-01-20 DOI: 10.4103/japtr.japtr_388_22

Begum Fauziyah, Dyah Fatimatussholichah, Aldila Raudatus Syarifah, Burhan Ma'arif

{"title":"The effect of additional sucrose on the value of tensile strength and elongation of chicken legs gelatin film.","authors":"Begum Fauziyah, Dyah Fatimatussholichah, Aldila Raudatus Syarifah, Burhan Ma'arif","doi":"10.4103/japtr.japtr_388_22","DOIUrl":"10.4103/japtr.japtr_388_22","url":null,"abstract":"Gelatin is a clear, flavorless, and colorless biopolymer formed by the denaturation of collagen protein in animal tissues. Gelatin is an important ingredient in the manufacture of capsule shell films. The tensile strength and elongation values of gelatin film indicate its quality. Sucrose is one of the ingredients that can affect the tensile strength and elongation of the gelatin film. To determine the effect of adding sucrose on the tensile strength and elongation of the chicken leg skin gelatin film. To extract the gelatin, clean, sliced chicken leg skin was freeze-dried, ground into powder, and extracted. The characteristics of chicken skin gelatin were measured using Fourier-transform infrared, and the data were read in the 4000-400 cm- 1 range for 32 scans. Pouring the solution into a mold, flattening it, and drying it at 40°C results in a film that has been tested for tensile strength and elongation. Gelatin films with a 30% sucrose addition had the best tensile strength and elongation values, with a tensile strength of 3.03 MPa and an elongation of 152.02%. Sucrose alters the tensile strength and elongation of chicken leg gelatin film. 30% sucrose provides better tensile and elongation strength.","PeriodicalId":14877,"journal":{"name":"Journal of Advanced Pharmaceutical Technology & Research","volume":"14 1","pages":"56-61"},"PeriodicalIF":1.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/2f/fc/JAPTR-14-56.PMC10026323.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9534660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0