Journal of Advanced Pharmaceutical Technology & Research最新文献_第8页

Probing antibacterial drugs for Fusobacterium nucleatum subsp. nucleatum ATCC 25586 targeting UDP-N-acetylglucosamine 1-carboxyltransferase. 核梭杆菌亚种抗菌药物的初步研究。靶向UDP-N-乙酰葡糖胺1-羧基转移酶的有核ATCC 25586。

IF 1.4

Journal of Advanced Pharmaceutical Technology & Research Pub Date : 2023-07-01 Epub Date: 2023-07-28 DOI: 10.4103/JAPTR.JAPTR_129_23

Dewi Saputri, Zaki Mubarak, Mudatsir Mudatsir, Inda Setyawati, Aprijal Ghiyas Setiawan, Mahdi Abrar

{"title":"Probing antibacterial drugs for Fusobacterium nucleatum subsp. nucleatum ATCC 25586 targeting UDP-N-acetylglucosamine 1-carboxyltransferase.","authors":"Dewi Saputri, Zaki Mubarak, Mudatsir Mudatsir, Inda Setyawati, Aprijal Ghiyas Setiawan, Mahdi Abrar","doi":"10.4103/JAPTR.JAPTR_129_23","DOIUrl":"10.4103/JAPTR.JAPTR_129_23","url":null,"abstract":"Fusobacterium nucleatum is a Gram-negative anaerobic bacteria that is commonly found in oral cavities and is associated with connective tissue destruction in periodontitis. UDP-N-acetylglucosamine 1-carboxyltransferase with enzyme commission number 2.5.1.7 is a transferases enzyme that plays a role in bacterial pathogenesis. Inhibiting binding sites of UDP-N-acetylglucosamine 1-carboxyltransferase is needed to find potential antibiotic candidates for periodontitis treatment. Hence, the research aimed to present potential UDP-N-acetylglucosamine 1-carboxyltransferase inhibiting compounds through molecular docking simulation by in silico analysis. DrugBank database was used to obtain the antibacterial candidates, which were further screened computationally using the AutoDock Vina program on Google Colab Pro. The top nine compounds yielded binding affinity ranging from -12.1 to -12.8 kcal/mol, with conivaptan as one of the three compounds having the highest binding affinity. Molecular dynamic study revealed that the ligand-protein complex for conivaptan had root-mean-square deviation values of 0.05-1.1 nm, indicating likeliness for stable interaction. Our findings suggest that conivaptan is the potent UDP-N-acetylglucosamine 1-carboxyltransferase inhibitor, hence its efficacy against periodontitis-causing bacteria.","PeriodicalId":14877,"journal":{"name":"Journal of Advanced Pharmaceutical Technology & Research","volume":"14 3","pages":"196-201"},"PeriodicalIF":1.4,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/7f/ff/JAPTR-14-196.PMC10483916.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10587667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retraction: Comparative study of male and female human hair: A microscopic analysis. 回缩：男性和女性头发的比较研究：显微镜分析。

IF 1.4

Journal of Advanced Pharmaceutical Technology & Research Pub Date : 2023-07-01 Epub Date: 2023-07-28 DOI: 10.4103/2231-4040.382188

引用次数: 0

Prevalence of hypertension links with body mass index by academic population in Babylon city. 巴比伦市学术人群的高血压患病率与体重指数相关。

IF 1.4

Journal of Advanced Pharmaceutical Technology & Research Pub Date : 2023-04-01 Epub Date: 2023-04-13 DOI: 10.4103/japtr.japtr_106_23

Zaid Saad Madhi, Mohannad Ali Hasan, Ali Abbas Hashim Almusawi

{"title":"Prevalence of hypertension links with body mass index by academic population in Babylon city.","authors":"Zaid Saad Madhi, Mohannad Ali Hasan, Ali Abbas Hashim Almusawi","doi":"10.4103/japtr.japtr_106_23","DOIUrl":"10.4103/japtr.japtr_106_23","url":null,"abstract":"Many risk factors have been listed that predispose to the occurrence of high blood pressure (BP). Although high body mass index (BMI) is a recognized risk factor for hypertension, the cutoff value for the high BMI was not taken into consideration as a predictor risk. There is no clear data on the occurrence of hypertension in Iraq in the highly educated population. Moreover, studies on hypertension in Iraq have been limited to a few studies. To assess and study the prevalence of BP in the academic population of Babylon city, a prospective study of 100 people was conducted. In the course of 3 months, during their work at the university in the morning, the questionnaire was filled out along with the body weight measurement. The receiver operating characteristic curve was used to measure the maximum area under the curve for the BMI score. Outcomes demonstrated that despite the high level of education, the prevalence of hypertension remains unacceptable. Awareness raising about the risk factors should be addressed through ongoing health education in health sectors and media. BMI of more than 25 could be considered a predictable risk value.","PeriodicalId":14877,"journal":{"name":"Journal of Advanced Pharmaceutical Technology & Research","volume":"14 2","pages":"133-136"},"PeriodicalIF":1.4,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/1f/34/JAPTR-14-133.PMC10226711.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9556835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

L-carnitine-loaded nanoparticle ameliorates cypermethrin-induced reproductive toxicity in adult male rats. L-肉碱负载纳米颗粒改善氯氰菊酯诱导的成年雄性大鼠生殖毒性。

IF 1.4

Journal of Advanced Pharmaceutical Technology & Research Pub Date : 2023-04-01 Epub Date: 2023-04-13 DOI: 10.4103/japtr.japtr_46_23

Noora Kadhim Hadi Alyasari, Wisam Hussein Selman

{"title":"L-carnitine-loaded nanoparticle ameliorates cypermethrin-induced reproductive toxicity in adult male rats.","authors":"Noora Kadhim Hadi Alyasari, Wisam Hussein Selman","doi":"10.4103/japtr.japtr_46_23","DOIUrl":"10.4103/japtr.japtr_46_23","url":null,"abstract":"The objective of this investigation was to find out whether L-carnitine-loaded nanoparticle (LCn) could reduce the reproductive toxicity of cypermethrin (CYP), the widely used insecticide in veterinary medicine in male rats. Twenty male Wistar rats that weighed between 210 and 240 g were split into four groups and treated daily for 2 months. The control group was given 0.9% normal saline solution daily. The second group received CYP (3.83 mg/kg b. w. p. o.) daily. The third group was administered with LCn and CYP (50 mg/kg b. wt. p. o. and 3.83 mg/kg b. wt. p. o., respectively) daily, whereas the fourth group received LCn alone (50 mg/kg b. wt. p. o.) daily. On day 60, all rats were sacrificed and samples were collected. CYP-treated animals exhibited inhibition of testicular anti-oxidative stress mechanisms, testicular steroidogenesis enzyme activity (3β-hydroxysteroid dehydrogenase [3β-HSD] and 17β-HSD), and downregulation of steroidogenic acute regulatory (StAR) gene expression. In addition, it decreased testosterone, follicle-stimulating hormone, and LH levels and had detrimental consequences for sperm quality. LCn attenuated CYP-induced reproductive toxicity via the alleviation of testicular oxidative stress status, improvement of steroidogenic enzyme activity, and upregulation of StAR gene expression, which are probably responsible for the concomitant improvement in testicular hormonal levels and improvement in sperm properties. Intriguingly, LCn treatment alone could enhance the functions of the male reproductive system.","PeriodicalId":14877,"journal":{"name":"Journal of Advanced Pharmaceutical Technology & Research","volume":"14 2","pages":"147-154"},"PeriodicalIF":1.4,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f0/e9/JAPTR-14-147.PMC10226707.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9908541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of ultrasound treatment of the skin on activation of Langerhans cells and antibody production in rodents. 超声波治疗皮肤对啮齿类动物郎格汉斯细胞活化和抗体产生的影响。

IF 1.4

Journal of Advanced Pharmaceutical Technology & Research Pub Date : 2023-04-01 Epub Date: 2023-04-13 DOI: 10.4103/japtr.japtr_647_22

Satoko Enjo, Yutaro Hazama, Soichiro Kimura, Yasunori Morimoto, Hideo Ueda

{"title":"Effect of ultrasound treatment of the skin on activation of Langerhans cells and antibody production in rodents.","authors":"Satoko Enjo, Yutaro Hazama, Soichiro Kimura, Yasunori Morimoto, Hideo Ueda","doi":"10.4103/japtr.japtr_647_22","DOIUrl":"10.4103/japtr.japtr_647_22","url":null,"abstract":"In this study, we investigated whether stimulating the skin with ultrasound (US) could activate Langerhans cells (LCs) - antigen-presenting cells in the epidermis and stimulate antibody production following the subcutaneous and intradermal injection of ovalbumin (OVA) in hairless rats and BALB/c mice. Three different US frequencies (20 kHz, 1, and 3 MHz) were used and the expression of langerin was monitored as a marker for the activation of LCs in the epidermal sheet. In hairless rats, the langerin signal peaked at 12 h post-US treatment and returned to control levels at 24 h. Its expression increased with increasing irradiation time, up to 20 min, and 20 kHz US induced the highest langerin expression among the three frequencies tested. These results were reproduced in BALB/c mice. When the skin was pretreated with 20 kHz US at 0.41 W/cm2 for 10 min, the production of OVA-specific immunoglobulin G1 in mice increased by 2.8- and 3.4-fold 28 days after subcutaneous or intradermal OVA injections, respectively. These findings indicate that stimulating the skin with US can trigger skin immune responses, leading to effective antigen-specific antibody production. US-assisted transdermal vaccine delivery delivers antigens to the skin and evokes an immune response, providing an effective noninvasive immunization strategy.","PeriodicalId":14877,"journal":{"name":"Journal of Advanced Pharmaceutical Technology & Research","volume":"14 2","pages":"94-98"},"PeriodicalIF":1.4,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/1d/89/JAPTR-14-94.PMC10226705.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9606371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The impact of the diode laser 940 nm photoactivated bleaching on color change of different composite resin restorations. 二极管激光940nm光活化漂白对不同复合树脂修复体颜色变化的影响。

IF 1.4

Journal of Advanced Pharmaceutical Technology & Research Pub Date : 2023-04-01 Epub Date: 2023-04-13 DOI: 10.4103/japtr.japtr_45_23

Amal Alaa Mawlood, Niaz H Hamasaeed

{"title":"The impact of the diode laser 940 nm photoactivated bleaching on color change of different composite resin restorations.","authors":"Amal Alaa Mawlood, Niaz H Hamasaeed","doi":"10.4103/japtr.japtr_45_23","DOIUrl":"10.4103/japtr.japtr_45_23","url":null,"abstract":"The study's main objective was to evaluate as well as compare the impact of diode laser 940 nm and conventional in-office bleaching technique on the color change of different composite resin restorations. A total of ninety composite resin discs (CRDs) were made from Filtek™ Bulk Fill, nanohybrid composite resin Filtek™ Z550 XT Universal Restorative, and flowable composite resin restoration Filtek™ Z350xt Flowable Composite. Group A: (30) CRD for Filtek™ Bulk Fill, group B: (30) CRD for Filtek™ Z550 XT Universal Restorative, and group C: (30) CRD for Filtek™ Z350xt Flowable Composite. A1, B1, and C1: (10 CRD in each group): Control group without bleaching. A2, B2, and C2: 10 CRD in each group bleached with Laser White20 without laser activation. A3, B3, and C3: 10 CRD in each group bleached with Laser White20 and activated by diode laser 940 nm. The color was evaluated using a dental spectrophotometer. One-way ANOVA was utilized for the comparison of the color stability of the CRD. P < 0.05 is regarded as statically significant. Both conventional and diode laser in-office vital tooth bleaching protocols affected the color stability of composite resin restorations. The result demonstrated that bleaching without laser activation produced color change (mean [8.30], standard deviation [SD] (1.95) P [< 0.001]), whereas the bleaching and activation by diode laser produced color change (mean [8.11], SD [1.99], P [< 0.001]). Both types of bleaching protocol affected composite resin restorations' color stability. In all tested materials, color changes after the bleaching protocol were clinically perceptible. The restorations following the bleaching procedure are required to be replaced by clinicians.","PeriodicalId":14877,"journal":{"name":"Journal of Advanced Pharmaceutical Technology & Research","volume":"14 2","pages":"155-160"},"PeriodicalIF":1.4,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a4/11/JAPTR-14-155.PMC10226697.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9551417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Method development, validation, and impurity measurement of β-estradiol from radiolabeled [¹³¹I]β-estradiol using radio-high-performance liquid chromatography for radioligand of saturation binding assay. 方法开发、验证和杂质测量放射性标记的[131I]β-雌二醇中的β-雌醇，使用放射性高效液相色谱法进行饱和结合分析的放射性配体。

IF 1.4

Journal of Advanced Pharmaceutical Technology & Research Pub Date : 2023-04-01 Epub Date: 2023-04-13 DOI: 10.4103/japtr.japtr_624_22

Isti Daruwati, Abednego Kristande Gwiharto, Hendris Wongso, Tri Hanggono Achmad, Mukh Syaifudin, Muchtaridi Muchtaridi

{"title":"Method development, validation, and impurity measurement of β-estradiol from radiolabeled [131I]β-estradiol using radio-high-performance liquid chromatography for radioligand of saturation binding assay.","authors":"Isti Daruwati, Abednego Kristande Gwiharto, Hendris Wongso, Tri Hanggono Achmad, Mukh Syaifudin, Muchtaridi Muchtaridi","doi":"10.4103/japtr.japtr_624_22","DOIUrl":"10.4103/japtr.japtr_624_22","url":null,"abstract":"β-estradiol is an estrogen steroid hormone and acts as an estrogen receptor agonist. Radiolabeled β-estradiol is widely used as a radioligand for binding assays. In this present study, the synthesis of [131I]β-estradiol has been successfully carried out. Accordingly, the measurement of the radiochemical purity (RCP) value and the presence of chemical impurities are needed. To validate the method for identifying the RCP and chemical impurities from [131I]β-estradiol using high-performance liquid chromatography (HPLC). The synthesis of [131I]β-estradiol was accomplished by a radioiodination reaction, and the RCP was determined by radio-HPLC. The method for β-estradiol measurement was validated by reversed-phase HPLC radio-analytical employing ultraviolet-visible (UV-Vis) and radioactive detector. The method for radio-HPLC analysis was validated and established using a C-18 column and MeCN: H2O (55:45 v/v) as the mobile phase. The following conditions were applied: a flow rate of 1.2 mL/min, isocratic, and a UV-Vis detector at 280 nm. The RCP of [131I]β-estradiol measured by thin-layer chromatography and radio-HPLC was 99.27% ± 1.25% and 95.75% ± 2.41%, respectively. The validation parameters were appropriate and met the requirements for acceptance. HPLC analysis was able to identify the presence of unlabeled estradiol (24.51%-27.29%) in the mixture of [131I]β-estradiol. As a result, purification using preparative HPLC or other methods will be required in future studies.","PeriodicalId":14877,"journal":{"name":"Journal of Advanced Pharmaceutical Technology & Research","volume":"14 2","pages":"105-112"},"PeriodicalIF":1.4,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/12/2e/JAPTR-14-105.PMC10226700.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9553588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of microwave-assisted extraction on polyphenol metabolite from Eleutherine bulbosa (Mill.) urb. bulbs using response surface methodology. 微波辅助提取球藻多酚代谢产物的工艺优化。使用响应面方法的灯泡。

IF 1.4

Journal of Advanced Pharmaceutical Technology & Research Pub Date : 2023-04-01 Epub Date: 2023-04-13 DOI: 10.4103/japtr.japtr_613_22

Islamudin Ahmad, Angga Cipta Narsa, M Riki Ramadhani, Nur Masyithah Zamruddin, Iswahyudi Iswahyudi, Hajrah Hajrah, Niken Indriyanti, M Arifuddin, Siska Siska, Supandi Supandi, Neneng Siti Silfi Ambarwati

{"title":"Optimization of microwave-assisted extraction on polyphenol metabolite from Eleutherine bulbosa (Mill.) urb. bulbs using response surface methodology.","authors":"Islamudin Ahmad, Angga Cipta Narsa, M Riki Ramadhani, Nur Masyithah Zamruddin, Iswahyudi Iswahyudi, Hajrah Hajrah, Niken Indriyanti, M Arifuddin, Siska Siska, Supandi Supandi, Neneng Siti Silfi Ambarwati","doi":"10.4103/japtr.japtr_613_22","DOIUrl":"10.4103/japtr.japtr_613_22","url":null,"abstract":"Eleutherine bulbosa bulbs, an endemic plant in Indonesia, have enormous potential as raw materials for pharmaceutical products. Therefore, it is necessary to strengthen and develop extraction methods that are easy, rapid, and efficient to enrich targeted secondary metabolites. This study aims to optimize the microwave-assisted extraction (MAE) method conditions for polyphenol metabolite from E. bulbosa bulbs. The MAE method (with different conditions) was applied to extract total polyphenol content (TPC) from E. bulbosa bulbs. TPC values were determined using a 96-well microplate reader spectrophotometry method and Folin-Ciocalteu reagent. The variables of MAE, as an experimental design-independent variable, were involved. The MAE method condition was optimized using response surface methodology (RSM) and Box-Behnken design based on the TPC value. The MAE condition was optimized with 60% ethanol, sample-solvent ratio of 1:10 g/mL, and 50% Watts of microwave power for 10 min. The quadratic regression analysis was achieved to predict the TPC value using the equation: TPC value = 28.63-5.545A +2.211B -0.741C +1.995D - 4.045AB +0.856AC -7.541BC +1.961CD -8.342A2-0.071B2 +1.840C2-1.535D2. For the scale-up confirmation test, a 50-g sample was used to prove the validity of the equation to predict the TPC value, yielding 35.33 ± 2.13 mg gallic acid equivalent/g samples. The optimum of the MAE condition recommended based on the results of RSM analysis can be applied directly to the enrichment of polyphenols metabolite constituent of E. bulbosa easily, cheaply, quickly, and efficiently.","PeriodicalId":14877,"journal":{"name":"Journal of Advanced Pharmaceutical Technology & Research","volume":"14 2","pages":"113-118"},"PeriodicalIF":1.4,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/6b/88/JAPTR-14-113.PMC10226706.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9553589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The radical scavenging activity of vanillin and its impact on the healing properties of wounds. 香兰素的自由基清除活性及其对伤口愈合性能的影响。

IF 1.4

Journal of Advanced Pharmaceutical Technology & Research Pub Date : 2023-04-01 Epub Date: 2023-04-13 DOI: 10.4103/japtr.japtr_631_22

Chutima Sinsuebpol, Kanokporn Burapapadh, Verisa Chowjaroen, Narumon Changsan

{"title":"The radical scavenging activity of vanillin and its impact on the healing properties of wounds.","authors":"Chutima Sinsuebpol, Kanokporn Burapapadh, Verisa Chowjaroen, Narumon Changsan","doi":"10.4103/japtr.japtr_631_22","DOIUrl":"10.4103/japtr.japtr_631_22","url":null,"abstract":"Vanillin, an extract from the Vanilla planifolia plant, is reported to possess potent antioxidant properties. The ability of vanillin to protect skin cells from reactive oxygen species (ROS)-induced damage and its potential use in the treatment of wounds were studied. Cytocompatibility and cytoprotective properties against ROS-induced damage were examined using keratinocyte and fibroblast cell models. Vanillin's effect on cell migration was studied using the scratch wound healing assay. Vanillin exhibited cytocompatibility and cytoprotective properties against cell damage induced by ROS. Human keratinocytes and fibroblast cells showed >80% survival when exposed to vanillin (10-500 μM). Both cells showed no evidence of necrosis or apoptosis, which was confirmed by acridine orange/propidium iodide staining. Both examined cells were exposed to 750 μM hydrogen peroxide to cause oxidative stress, and vanillin demonstrated the ability to inhibit ROS-induced cell death. In addition, a considerable increase in cell migration suggested that vanillin had the ability to heal wounds in vitro. Vanillin is safe and potentially useful in wound healing treatments.","PeriodicalId":14877,"journal":{"name":"Journal of Advanced Pharmaceutical Technology & Research","volume":"14 2","pages":"99-104"},"PeriodicalIF":1.4,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/32/31/JAPTR-14-99.PMC10226704.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9908544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Solvent preextraction influenced to coumarin and glucose binding capacity of cinnamomi's extracts. 溶剂预处理对肉桂提取物香豆素和葡萄糖结合能力的影响。

IF 1.4

Journal of Advanced Pharmaceutical Technology & Research Pub Date : 2023-04-01 Epub Date: 2023-04-13 DOI: 10.4103/japtr.japtr_671_22

Martha Ervina, Virgiana Kristin Coco Wea, Annisah Oktaviani, Lanny Hartanti, Restry Sinansari, Yufita Ratnasari Wilianto, Steven, Caroline

{"title":"Solvent preextraction influenced to coumarin and glucose binding capacity of cinnamomi's extracts.","authors":"Martha Ervina, Virgiana Kristin Coco Wea, Annisah Oktaviani, Lanny Hartanti, Restry Sinansari, Yufita Ratnasari Wilianto, Steven, Caroline","doi":"10.4103/japtr.japtr_671_22","DOIUrl":"10.4103/japtr.japtr_671_22","url":null,"abstract":"Cinnamon is one of the world's oldest spices that is also widely used as medicine for antimicrobe, anti-inflammation, and immune stimulant to now developed as an antidiabetic herbal medicine. Among its bioactive contents, the coumarin must have been controlled; since of its toxicities. Therefore, in this study aimed to reduce the amount of coumarin in the extracts by chloroform preextraction to dried powdered cinnamons and determine their glucose binding capacity. The extractions were used two methods by water infusion and ethanol soxhletation. To measure the coumarin's was used validated thin-layer chromatography (TLC)-densitometry, while for the chemical profiling of the extract was examined with liquid chromatography-mass spectrometry. The activity antidiabetic of the extracts was obtained by glucose binding. The TLC-densitometry method has been validated with silica gel 60F254 and n-hexane: ethyl acetate (8:2, v/v) systems. The coumarin's spot was observed at a wavelength of 285 nm on retention factor (Rf) 0.33, with tailings factor 1. The intraday and interday linearities tests showed a linear response result. The recovery value, coefficient of variation, and detection and a quantitation limit were met the standard requirements, respectively. Moreover, the results were observed (1) the solvent preextraction may reduce the coumarin content, (2) the coumarin content in the ethanol extract was higher than in the infusion, and (3) the preextraction solvents would reduce the glucose-binding capacity in ethanol and water cinnamomi's extract. These results may be developed further and applied for producing cinnamon's free coumarin extracts.","PeriodicalId":14877,"journal":{"name":"Journal of Advanced Pharmaceutical Technology & Research","volume":"14 2","pages":"69-74"},"PeriodicalIF":1.4,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ca/e4/JAPTR-14-69.PMC10226712.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9908546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0