Journal of Advanced Pharmaceutical Technology & Research最新文献

Erratum: Preparation and evaluation of topical span 60-based oleogel of voriconazole. 更正：局部用伏立康唑油凝胶的制备和评价。

IF 1.4

Journal of Advanced Pharmaceutical Technology & Research Pub Date : 2025-04-01 Epub Date: 2025-05-19 DOI: 10.4103/JAPTR.JAPTR_120_25

引用次数: 0

Protective effect of fermented vegetable compounds against nonalcoholic fatty liver disease using metabolite profiling, integrated network pharmacology, and molecular docking approach. 利用代谢物谱、综合网络药理学和分子对接方法研究发酵蔬菜化合物对非酒精性脂肪肝的保护作用

IF 1.4

Journal of Advanced Pharmaceutical Technology & Research Pub Date : 2025-04-01 Epub Date: 2025-05-19 DOI: 10.4103/JAPTR.JAPTR_331_24

Ermin Rachmawati, Larasati Sekar Kinasih, Nabila Rahmadani, Tias Pramesti Griana, Roihatul Muti'ah, Suharti Suharti, Dwiki Pramudika Abdul Azis, Endah Eni

{"title":"Protective effect of fermented vegetable compounds against nonalcoholic fatty liver disease using metabolite profiling, integrated network pharmacology, and molecular docking approach.","authors":"Ermin Rachmawati, Larasati Sekar Kinasih, Nabila Rahmadani, Tias Pramesti Griana, Roihatul Muti'ah, Suharti Suharti, Dwiki Pramudika Abdul Azis, Endah Eni","doi":"10.4103/JAPTR.JAPTR_331_24","DOIUrl":"10.4103/JAPTR.JAPTR_331_24","url":null,"abstract":"Vegetable fermentation extract (VFE) shows potential as a preventive agent to inhibit nonalcoholic fatty liver disease (NAFLD). This study identified bioactive compounds of VFE and explored the mechanism of VFE against NAFLD. Metabolite profiling was analysed using Liquid chromatography-tandem mass spectrometry (LC-MS/MS). The bioactive compounds were screened using the Lipinski Rule of 5, toxicity, and biological activity prediction, followed by network pharmacology and molecular docking. Of the 24 bioactive compounds identified, 8 compounds passed the screening process. Twenty-four genes from network pharmacology were involved in the NAFLD mechanism, including those related to insulin signaling, inflammation, and lipid metabolism. Kaempferol, apigenin, and N-(p-coumaroyl) serotonin showed a good binding affinity with CCR2, AKT, IL-6, and PPAR-γ compared to simvastatin and metformin. Bioactive compounds from VFE were predicted to ameliorate NAFLD.","PeriodicalId":14877,"journal":{"name":"Journal of Advanced Pharmaceutical Technology & Research","volume":"16 2","pages":"92-98"},"PeriodicalIF":1.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12156115/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144284431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anticancer activity prediction of Curcuma longa and Phyllanthus urinaria through computational analysis. 基于计算分析的姜黄、余叶草抗癌活性预测。

IF 1.4

Journal of Advanced Pharmaceutical Technology & Research Pub Date : 2025-04-01 Epub Date: 2025-05-19 DOI: 10.4103/JAPTR.JAPTR_209_24

Marisca Evalina Gondokesumo, Muhammad Rezki Rasyak, Mansur Ibrahim

{"title":"Anticancer activity prediction of Curcuma longa and Phyllanthus urinaria through computational analysis.","authors":"Marisca Evalina Gondokesumo, Muhammad Rezki Rasyak, Mansur Ibrahim","doi":"10.4103/JAPTR.JAPTR_209_24","DOIUrl":"10.4103/JAPTR.JAPTR_209_24","url":null,"abstract":"Traditional Indonesian medicine has long been recognized for its curative qualities, although concerns remain over the efficacy and safety of medicinal herbs. The application of computational methods in novel drug discovery is one of the promising new insights offered by recent technical advancements. This study attempts to find putative anticancer chemicals in two extensively used plants in Southeast Asia, Curcuma longa and Phyllanthus urinaria, using a computational technique. AKT1, a model protein implicated in the development of cancer cells, was used in this investigation. In these two plants, 28 different chemicals were found. We use strict selection standards, like Lipinski's rule of five, to ensure the identification of potential candidates. The findings demonstrated that 24 compounds had comparable binding affinities to the reference ligands, indicating encouraging therapeutic potential. Subsequent investigation showed that the compounds' chemical structures differed and that their similarities to the reference ligand were <10%. However, for both plant-derived drugs, the amino acid binding patterns revealed remarkable similarities that went above 50% similarity, suggesting that both may be useful.","PeriodicalId":14877,"journal":{"name":"Journal of Advanced Pharmaceutical Technology & Research","volume":"16 2","pages":"53-60"},"PeriodicalIF":1.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12156121/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144284403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of microemulsion containing thai herbal remedy extract for treatment of urticaria. 泰国草药提取物治疗荨麻疹微乳剂的研制。

IF 1.4

Journal of Advanced Pharmaceutical Technology & Research Pub Date : 2025-04-01 Epub Date: 2025-05-19 DOI: 10.4103/JAPTR.JAPTR_267_24

Natta Choedchutirakul, Intouch Sakpakdeejaroen, Sumalee Panthong

{"title":"Development of microemulsion containing thai herbal remedy extract for treatment of urticaria.","authors":"Natta Choedchutirakul, Intouch Sakpakdeejaroen, Sumalee Panthong","doi":"10.4103/JAPTR.JAPTR_267_24","DOIUrl":"10.4103/JAPTR.JAPTR_267_24","url":null,"abstract":"Herbal medicine can be used as an alternative treatment to alleviate allergy symptoms in individuals with urticaria. The herbal remedy comprised four plant components, including Allium ascalonicum, Acanthus ilicifolius, Bambusa blumeana, and Rhinacanthus nasutus, in equal amounts. It was administered to impacted areas of the skin affected by allergies. Nevertheless, the disadvantages of herbal products include their limited ability to penetrate the skin. Microemulsion (ME) is a topical medication delivery device that enhances drug absorption into the skin. This work aims to develop and optimize a ME containing an herbal remedy extract. The construction methods utilized were pseudo-ternary phase diagrams and mixture design using system engineering software. Data were calculated and reported as means ± standard. Statistical significance was indicated when P < 0.05. The optimal herbal ME consisted of 4%-5% isopropyl myristate, 35%-45% Smix, and 46%-58% water. The stability studies demonstrated consistent physical properties and a low level of viscosity. The pH, particle size, polydispersity index, and zeta potential readings have remained stable after storage under nine heating and cooling cycles. The ME exhibited sustained release of rhinacanthin-C, with a steady release rate of 10.34% ± 0.03% from 0.5 h to 20.21% ± 0.11% at 8 h. The Kae-Lom-Pid proves to be suited for MEs and can serve as a model for pharmaceutical development.","PeriodicalId":14877,"journal":{"name":"Journal of Advanced Pharmaceutical Technology & Research","volume":"16 2","pages":"66-72"},"PeriodicalIF":1.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12156114/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144284406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exopolysaccharide of goat milk kefir as an anti-obesity agent: Inhibition of adipogenesis enzyme activity on 3T3-L1 adipocyte model cells. 羊乳开非尔外多糖抗肥胖作用：对3T3-L1脂肪细胞模型细胞脂肪生成酶活性的抑制作用。

IF 1.4

Journal of Advanced Pharmaceutical Technology & Research Pub Date : 2025-04-01 Epub Date: 2025-05-19 DOI: 10.4103/JAPTR.JAPTR_169_24

Lilik Eka Radiati, Evy Damayanthi, Armaini Armaini, Aman Santoso, Dian Laksamana Hati, Dedi Fardiaz, Laprianika Retha Hapita Sari, Ahmad Khoirul Umam

{"title":"Exopolysaccharide of goat milk kefir as an anti-obesity agent: Inhibition of adipogenesis enzyme activity on 3T3-L1 adipocyte model cells.","authors":"Lilik Eka Radiati, Evy Damayanthi, Armaini Armaini, Aman Santoso, Dian Laksamana Hati, Dedi Fardiaz, Laprianika Retha Hapita Sari, Ahmad Khoirul Umam","doi":"10.4103/JAPTR.JAPTR_169_24","DOIUrl":"10.4103/JAPTR.JAPTR_169_24","url":null,"abstract":"Obesity is increasingly a leading cause of dyslipidemia and metabolic syndrome, which are known to be comorbidities of various diseases. A study was conducted to evaluate the effects of different concentrations of exopolysaccharide from goat milk kefir (EPS-GMK) on the activity of key enzymes involved in fat cell formation in 3T3-L1 adipocyte cells. The study used six groups, including two control groups (negative and positive) and four treatment groups with varying concentrations of EPS-GMK (25, 50, 75, and 100 µg/ml). The results showed that EPS-GMK has a significant inhibitory effect on the activity of key enzymes involved in fat cell formation. The findings suggest that EPS-GMK could be a valuable component in developing anti-obesity functional foods. This study aims to analyze the effects of EPS-GMK on the 3T3-L1 adipocyte cells' phosphoenolpyruvate carboxykinase, Glucose-6-Phosphate Dehydrogenase, and Lipoprotein lipase administration. This study applied different doses of EPS-GMK to 3T3-L1 adipocytes with four replications. The doses used were P1 (25 g/ml), P2 (50 g/ml), P3 (75 g/ml), and P4 (100 g/ml). The study also included two control groups, negative control and positive control. This study utilized a completely randomized design for maximum efficacy. The experimental material was 3T3-L1 adipocyte cells, each sample containing adipocyte model cells. One hundred six cells per well were induced with DMI on 24 culture plates. Statistical variance analysis was used to determine the significance between the mean values. The study results revealed that EPS-GMK concentrations between 25 and 100 g/mL could potentially reduce adipogenesis enzyme activity. According to this study, EPS-GMK has the potential and feasibility of being used as an anti-obesity agent.","PeriodicalId":14877,"journal":{"name":"Journal of Advanced Pharmaceutical Technology & Research","volume":"16 2","pages":"47-52"},"PeriodicalIF":1.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12156120/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144284409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hypoglycemic episodes exacerbate skeletal muscle insulin resistance in type 2 diabetes mellitus. 低血糖发作加重2型糖尿病骨骼肌胰岛素抵抗。

IF 1.4

Journal of Advanced Pharmaceutical Technology & Research Pub Date : 2025-04-01 Epub Date: 2025-05-19 DOI: 10.4103/JAPTR.JAPTR_388_24

Hendra Zufry, Rosdiana Rosdiana, Krishna Wardhana Sucipto, Agustia Sukri Ekadamayanti, Sarah Firdausa

{"title":"Hypoglycemic episodes exacerbate skeletal muscle insulin resistance in type 2 diabetes mellitus.","authors":"Hendra Zufry, Rosdiana Rosdiana, Krishna Wardhana Sucipto, Agustia Sukri Ekadamayanti, Sarah Firdausa","doi":"10.4103/JAPTR.JAPTR_388_24","DOIUrl":"10.4103/JAPTR.JAPTR_388_24","url":null,"abstract":"To date, the specific impact of hypoglycemia on insulin resistance in skeletal muscle is unclear. This study aimed to investigate the correlation between hypoglycemia in individuals with Type 2 Diabetes Mellitus by assessing peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) and Akt levels in both skeletal muscle tissue and blood plasma. A true experimental-design study was conducted at the Laboratory of Animal Clinical Study, Faculty of Veterinary, Universitas Syiah Kuala, from October to December 2022. The study utilized 24 male rats (Rattus norvegicus) and the rates were categorized into the following groups: control (K1), streptozocin-induced diabetic without hypoglycemia (K2), streptozocin-induced diabetic with mild hypoglycemia (K3), and streptozocin-induced diabetic with severe hypoglycemia (K4). The rats were euthanized a minimum of 30 min after hypoglycemia was confirmed. W Quadriceps femoris muscle and 2 ml of heart blood were collected. PGC-1α and protein kinase B/Akt were examined using enzyme-linked immunosorbent assay. This study demonstrates that PGC-1α and Akt levels in skeletal muscle and plasma are influenced by hypoglycemia severity in rats, with lower levels associated with more severe hypoglycemia, though no significant differences were observed between mild and severe hypoglycemia groups. A positive correlation was found between PGC-1α and Akt levels in skeletal muscle and plasma, suggesting interdependency. Control group plasma levels were 2.48 ± 0.53 ng/ml for PGC-1α and 11.26 ± 1.21 ng/ml for Akt. Even mild episodes of hypoglycemia can lead to a substantial deterioration of insulin resistance in skeletal muscle.","PeriodicalId":14877,"journal":{"name":"Journal of Advanced Pharmaceutical Technology & Research","volume":"16 2","pages":"99-105"},"PeriodicalIF":1.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12156113/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144284410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential of rutin from Rhizophora mucronata leaves as a Inhibitor of Kelch-like ECH-associated protein 1/Nuclear factor erythroid 2 related factor 2 Keap1/Nrf2): An in silico study for Alzheimer's therapy. 根参叶芦丁作为kelch样ech相关蛋白1/核因子红系2相关因子2 （Keap1/Nrf2）抑制剂的潜力：一项治疗阿尔茨海默病的计算机研究。

IF 1.4

Journal of Advanced Pharmaceutical Technology & Research Pub Date : 2025-04-01 Epub Date: 2025-05-19 DOI: 10.4103/JAPTR.JAPTR_313_24

Legis Ocktaviana Saputri, Lina Permatasari, Herpan Syafii Harahap, Rohadi Muhammad Rosyidi, Arina Windri Rivarti, Lale Maulin Prihatina, Zilfia Rahayu, Wiwin Azariani

{"title":"Potential of rutin from Rhizophora mucronata leaves as a Inhibitor of Kelch-like ECH-associated protein 1/Nuclear factor erythroid 2 related factor 2 Keap1/Nrf2): An in silico study for Alzheimer's therapy.","authors":"Legis Ocktaviana Saputri, Lina Permatasari, Herpan Syafii Harahap, Rohadi Muhammad Rosyidi, Arina Windri Rivarti, Lale Maulin Prihatina, Zilfia Rahayu, Wiwin Azariani","doi":"10.4103/JAPTR.JAPTR_313_24","DOIUrl":"10.4103/JAPTR.JAPTR_313_24","url":null,"abstract":"Alzheimer's disease (AD) is the most prevalent type of dementia, negatively affecting the overall quality of life. Targeting the nuclear factor erythroid 2-related factor 2 (Nrf2) pathway, a regulator of protective genes, offers potential AD therapies. Inhibiting Kelch-like ECH-associated protein 1 (Keap1), which suppresses Nrf2, may help reduce neuronal damage. Rhizophora mucronata, a mangrove known for its anti-inflammatory and antioxidant properties, contains rutin, which is a promising potential AD therapy. The study aimed to explore the potential of rutin, a compound from R. mucronata leaves, to inhibit the Keap1-Nrf2. The study analyzed the metabolomic profile of R. mucronata leaves and evaluated their compound, rutin, as AD therapeutic potential via the Keap1-Nrf2 pathway using in silico molecular docking. R. mucronata was extracted using 96% ethanol. Metabolomic analysis employed liquid chromatography-high resolution mass spectrometry (LC-HRMS). The in silico simulations used BIOVIA Discovery Studio and AutoDock Tool 1.5.7 for docking rutin and donepezil with the Keap1. Docking results were evaluated based on binding energy scores and inhibition constant. HRMS identified hundreds of compounds, with quercetin and rutin as major flavonoids. Molecular docking indicated rutin and donepezil had a binding energy of -6.97 ± 0.16 kcal/mol and -7.63 ± 0.04 kcal/mol, respectively. Their amino acid interaction was similar. R. mucronata leaf extract, particularly rutin, showed promise as an AD therapeutic agent through the Keap1-Nrf2 pathway, warranting further research.","PeriodicalId":14877,"journal":{"name":"Journal of Advanced Pharmaceutical Technology & Research","volume":"16 2","pages":"73-79"},"PeriodicalIF":1.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12156112/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144284430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibacterial efficacy of the cassava (Manihot esculenta C.) leaf and pirdot (Saurauia vulcani K.) leaf extract combination against Staphylococcus aureus. 木薯（Manihot esculenta C.）叶和pirdot （Saurauia vulcani K.）叶提取物组合对金黄色葡萄球菌的抗菌作用。

IF 1.4

Journal of Advanced Pharmaceutical Technology & Research Pub Date : 2025-04-01 Epub Date: 2025-05-19 DOI: 10.4103/JAPTR.JAPTR_3_24

Muhammad Toni, Yunita Sari Pane

{"title":"Antibacterial efficacy of the cassava (Manihot esculenta C.) leaf and pirdot (Saurauia vulcani K.) leaf extract combination against Staphylococcus aureus.","authors":"Muhammad Toni, Yunita Sari Pane","doi":"10.4103/JAPTR.JAPTR_3_24","DOIUrl":"10.4103/JAPTR.JAPTR_3_24","url":null,"abstract":"Staphylococcus aureus is a common bacterium causing various infections. The irrational use of antibiotics can lead to resistance, prompting the exploration of alternative treatments, such as herbal plants like cassava and pirdot leaves. To evaluate the antibacterial effectiveness of a combination of cassava leaf and pirdot leaf extracts against S. aureus. Antibacterial activity was tested using the different concentration ratios of cassava and pirdot leaf extracts (80:20, 50:50, and 30:70), with chloramphenicol (30 µg) as the positive control and DMSO (10%) as the negative control. \"The inhibition zones for the various concentrations were as follows: 80% cassava and 20% pirdot (14.14 ± 1.69 mm), 50% cassava and 50% pirdot (26.47 ± 2.15 mm), and 30% cassava and 70% pirdot (22.73 ± 1.57 mm). The positive control (chloramphenicol) showed an inhibition zone of 43.59 ± 1.03 mm. Statistical analysis (Kruskal-Wallis, P = 0.0001) indicated significant differences among all treatment groups, followed by Dunn's test for pairwise comparisons. The inhibition zones for the various concentrations were as follows: 80% cassava and 20% pirdot (14.14 ± 1.69 mm), 50% cassava and 50% pirdot (26.47 ± 2.15 mm), and 30% cassava and 70% pirdot (22.73 ± 1.57 mm). The positive control (chloramphenicol) had an inhibition zone of 43.59 ± 1.03 mm. Statistical analysis (Kruskal-Wallis, P = 0.0001) indicated significant differences between the treatment groups.","PeriodicalId":14877,"journal":{"name":"Journal of Advanced Pharmaceutical Technology & Research","volume":"16 2","pages":"61-65"},"PeriodicalIF":1.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12156118/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144284402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cytotoxicity test and the potency of polyvinyl alcohol-based Moringa oleifera nanoparticles on cancer cell death: In vitro study. 聚乙烯醇基辣木纳米颗粒对癌细胞死亡的细胞毒性试验和效力：体外研究。

IF 1.4

Journal of Advanced Pharmaceutical Technology & Research Pub Date : 2025-04-01 Epub Date: 2025-05-19 DOI: 10.4103/JAPTR.JAPTR_12_25

Vania Syahputri, Theresia Indah Budhy, Hani Plumeriastuti, Tengku Natasha Eleena Binti Tengku Ahmad Noor

{"title":"Cytotoxicity test and the potency of polyvinyl alcohol-based Moringa oleifera nanoparticles on cancer cell death: In vitro study.","authors":"Vania Syahputri, Theresia Indah Budhy, Hani Plumeriastuti, Tengku Natasha Eleena Binti Tengku Ahmad Noor","doi":"10.4103/JAPTR.JAPTR_12_25","DOIUrl":"10.4103/JAPTR.JAPTR_12_25","url":null,"abstract":"Moringa oleifera (MO) has been explored for anticancer drug development. However, conventional extract formulations face limitations in drug delivery. Nanoparticles offer a promising alternative due to their small size, enhancing drug selectivity, efficacy, and safety. Therefore, this study aimed to characterize polyvinyl alcohol-based MO nanoparticles (NpMO) and assess their cytotoxicity and anticancer potential. Moreover, NpMO was synthesized using ultrasonication and characterized by its size, functional groups, and surface morphology. Then, an MTT assay was conducted in Vero and HeLa cells, each divided into a control group and five treatment groups (PV1-5 and PL1-5). The treatment groups received NpMO with various doses: 12.5 µg/mL, 25 µg/mL, 50 µg/mL, 100 µg/mL, and 200 µg/mL. The results were represented as OD values and percentage of viable cells, with statistical analysis performed using SPSS version 27. We found that Vero cell viability remained high at 96%, 95%, 93%, 90%, and 82.3% in PV groups, indicating no significant difference between control and PV1-PV4 groups with statistical analysis. Meanwhile, HeLa cell viability decreased to 98%, 92%, 78%, 69%, and 50.2%, with PL5 showing the lowest viability percentage. Statistical analysis confirmed a significant difference between PL5 and the other PL groups. In conclusion, NpMO showed minimal toxicity to Vero cells (>50% viability up to 200 µg/mL) but significantly reduced HeLa cell viability at 50-200 µg/mL, with the strongest effect at 200 µg/mL, indicating a potential anticancer activity.","PeriodicalId":14877,"journal":{"name":"Journal of Advanced Pharmaceutical Technology & Research","volume":"16 2","pages":"80-85"},"PeriodicalIF":1.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12156116/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144284405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cytoprotective effect and antioxidant activities of hesperidin against cisplatin-induced fibroblast cell. 橙皮苷对顺铂诱导成纤维细胞的细胞保护作用及抗氧化活性。

IF 1.4

Journal of Advanced Pharmaceutical Technology & Research Pub Date : 2025-04-01 Epub Date: 2025-05-19 DOI: 10.4103/JAPTR.JAPTR_298_24

Anif Nur Artanti, Fea Prihapsara, Faaza Aulia Rahman, Mukh Syaifudin, Muchtaridi Muchtaridi, Endah Puji Septisetyani, Edy Meiyanto, Okid Parama Astirin

{"title":"Cytoprotective effect and antioxidant activities of hesperidin against cisplatin-induced fibroblast cell.","authors":"Anif Nur Artanti, Fea Prihapsara, Faaza Aulia Rahman, Mukh Syaifudin, Muchtaridi Muchtaridi, Endah Puji Septisetyani, Edy Meiyanto, Okid Parama Astirin","doi":"10.4103/JAPTR.JAPTR_298_24","DOIUrl":"10.4103/JAPTR.JAPTR_298_24","url":null,"abstract":"Cisplatin (Cisp), a platinum-based compound, is a potent chemotherapy drug that effectively treats various cancers such as lung, breast, bladder, and hepatocellular carcinoma. However, its clinical application is limited due to its fibroblast damage, which is linked to its ability to produce collagen and other extracellular matrix components essential for tissue healing. Enhancing antioxidant capacity offers a potential strategy to reduce Cisp-induced fibroblast damage. Hesperidin (HSD), a flavonoid from Citrus sp., exhibits various pharmacological properties, including anti-inflammatory and antioxidant effects. This study aims to determine HSD as cytoprotective induced by Cisp using the fibroblast cell lines (NIH-3T3). 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay was used to determine antioxidant activity. The viability cell after treatment with HSD, Cisp, and cotreatment HSD-Cisp was evaluated through Mictroculure Tetrazolium Technique (MTT) assay. The evaluation of senescence was performed using the senescence-associated β-galactosidase assay. Gelatin zymography assay was utilized to analyze the activity of matrix metalloproteinases (MMPs). Data were analyzed using one-way ANOVA and Tukey's post hoc test in SPSS (version 20.0). The IC50 of the radical scavenging assay of HSD was found to be 20.967 ± 0.016 µM. HSD showed low cytotoxicity against NIH-3T3 cells, with IC50 values of over 500 µM. HSD showed an antagonistic effect when used as cotreatment HSD with Cisp in NIH-3T3 cells, with a combination index >1. Cotreatment of HSD and Cisp reduces cellular senescence and the expression of MMP-9 and MMP-2. These findings suggest that HSD could be beneficial as a cytoprotective agent, helping to maintain cellular health against chemotherapy.","PeriodicalId":14877,"journal":{"name":"Journal of Advanced Pharmaceutical Technology & Research","volume":"16 2","pages":"86-91"},"PeriodicalIF":1.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12156119/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144284404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0