Journal of Advanced Pharmaceutical Technology & Research最新文献

{"title":"Subchronic kretek smoke exposure induces myocardial p53 protein expression and associated inflammatory and fibrotic alterations in rats.","authors":"David Tjahyadi, Hosea Jaya Edy, Endrico Xavierees, Joey Joshua Vidova Tjahyadi, Laurentia Gabrielle, Mellysa Henggar Ratnasari, Marlita Sabta Utari, Ashaolu Victoria Oladimeji, Seçil Karahüseyin","doi":"10.4103/JAPTR.JAPTR_12_26","DOIUrl":"10.4103/JAPTR.JAPTR_12_26","url":null,"abstract":"<p><p>Kretek smoke contains various toxic substances that are harmful to human health and has been associated with inflammation and structural alterations in multiple organs. However, its effects on cardiac tissue remain incompletely understood. This study aimed to evaluate myocardial histopathological alterations and p53 protein expression following subchronic kretek smoke exposure in rats. Male Rattus norvegicus Sprague-Dawley rats were randomly divided into two groups. Group I was exposed to normal air, while Group II was exposed to kretek smoke at a dose of 1 kretek/rat/day for 30 days. Cardiac tissues were examined using H&E and MT staining. IHC analysis was performed to assess myocardial p53 protein expression, while ultrastructural observations of the myocardium were conducted using SEM. Group II rats exhibited myocardial inflammation characterized by lymphocyte infiltration and increased collagen accumulation, whereas Group I rats showed no inflammatory changes and minimal collagen deposition. Myocardial p53 protein expression was significantly higher in Group II (224.68 ± 5.44 µm²) than in Group I (199.87 ± 13.35 µm²; <i>P</i> = 0.005). Ultrastructural myocardial alterations were observed in Group II but not in Group I. In conclusion, subchronic kretek smoke exposure increases myocardial p53 protein expression accompanied by inflammatory and fibrotic alterations.</p>","PeriodicalId":14877,"journal":{"name":"Journal of Advanced Pharmaceutical Technology & Research","volume":"17 3","pages":"288-295"},"PeriodicalIF":1.4,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13537778/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148880500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>In vitro</i> antibacterial and concentration-dependent bactericidal effects of <i>Terminalia arjuna</i> extract against <i>Staphylococcus aureus</i> and methicillin-resistant <i>Staphylococcus aureus</i>, with synergistic activity against methicillin-resistant <i>Staphylococcus aureus</i>.","authors":"Sumalee Panthong, Jittiporn Chaorai, Pusaree Temboonkiat","doi":"10.4103/JAPTR.JAPTR_25_26","DOIUrl":"10.4103/JAPTR.JAPTR_25_26","url":null,"abstract":"<p><p><i>Staphylococcus aureus</i> infections, particularly methicillin-resistant <i>S. aureus</i> (MRSA), remain a major clinical challenge due to limited treatment options. Plant-derived compounds have gained interest as alternative or adjunct antibacterial agents. This study investigated the antibacterial, antibiofilm, and synergistic effects of <i>Terminalia arjuna</i> extract against <i>S. aureus</i> and MRSA, with high-performance liquid chromatography (HPLC) analysis. Antibacterial activity of <i>T. arjuna</i> extract was evaluated using microdilution, time-kill, checkerboard, and biofilm assays against <i>S. aureus</i> and MRSA, with chemical profiling by HPLC. Data were calculated and shown as means ± standard deviation. A Significant difference was marked when <i>P</i> < 0.05. The ethanolic extract of <i>T. arjuna</i> demonstrated bactericidal action against <i>S. aureus</i> and MRSA, with minimum inhibitory concentration (MIC) and minimum bactericidal concentration values of 625 μg/mL. A concentration-dependent effect was observed, with complete bacterial killing achieved at supra-MIC levels. The extract reduced biofilm biomass by more than 80% at low concentrations. Notably, a pronounced synergistic interaction with ampicillin against MRSA was observed, yielding a fractional inhibitory concentration index of 0.281. The <i>T. arjuna</i> extract exhibited <i>in vitro</i> antibacterial activity and showed concentration-dependent bactericidal effects, with a synergistic interaction with ampicillin observed against MRSA.</p>","PeriodicalId":14877,"journal":{"name":"Journal of Advanced Pharmaceutical Technology & Research","volume":"17 3","pages":"268-274"},"PeriodicalIF":1.4,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13537797/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148880409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Discovery of bioactive peptides from <i>Arbacia lixula</i> as dipeptidyl peptidase-IV inhibitors: An <i>in silico</i> approach.","authors":"Zuhrah Taufiqa, Benny Alexander Maisa, Kevin Nathaniel Cuandra, Enrico Franditho Sihombing, Muhammad Fathu Ridho, Sheryl Pricilla Daniela Elizabeth Silalahi, Vina Sari Nugrahaning Widi, Afifah Rahma Adila, Aurellia Zahra Quinneala, Aryas Hakim Indrajaya, Reka Febriani, Zahra Roidah Amalia Hasna, Sari Rahmadani, Erra Kurniasari, Mazaya Mansu Lubis, Amira Puji Hastuti","doi":"10.4103/JAPTR.JAPTR_41_26","DOIUrl":"10.4103/JAPTR.JAPTR_41_26","url":null,"abstract":"<p><p>Dipeptidyl peptidase-IV (DPP-IV) inhibition is a validated therapeutic mechanism for type 2 diabetes mellitus. Marine-derived bioactive peptides offer a diverse array of potential inhibitors but often exhibit pharmacokinetic limitations that impede clinical translation. This study aimed to identify and evaluate candidate peptides from <i>Arbacia lixula</i> as potential DPP-IV inhibitors using an integrated <i>in silico</i> approach. A total of twenty peptides were evaluated <i>in silico</i> for absorption, distribution, metabolism, excretion, and toxicity (ADMET) profiles using SwissADME, pkCSM, and ProTox. Molecular docking against DPP-IV was performed in MOE v2022.02 with linagliptin as the reference ligand; docking poses were evaluated by predicted binding energy and root mean square deviation (RMSD). Top-ranked peptide-DPP-IV complexes were subjected to 50 ns molecular dynamics (MD) simulations in YASARA Dynamics v4.3.13 to assess conformational stability. ADMET predictions showed low gastrointestinal absorption in most peptides, whereas Peptides 17, 18, and 20 showed high predicted absorption. No target-organ or endpoint-specific toxicity was predicted. Peptides 7, 15, and 18 were prioritized based on favorable docking scores, RMSD <2.0 Å, and relevant DPP-IV residue interactions. Docking scores were interpreted cautiously, not as direct quantitative comparisons with linagliptin. During 50 ns MD simulations, RMSD, root mean square fluctuation, radius of gyration, and solvent-accessible surface area profiles supported the stability, compactness, and dynamic consistency of selected peptide-DPP-IV complexes. Peptides 7, 15, and 18 showed favorable <i>in silico</i> predicting binding, relevant DPP-IV interactions, and acceptable preliminary safety profiles as potential DPP-IV inhibitory candidates. These findings should be interpreted as computational leads that require further <i>in vitro</i> and <i>in vivo</i> validation.</p>","PeriodicalId":14877,"journal":{"name":"Journal of Advanced Pharmaceutical Technology & Research","volume":"17 3","pages":"213-220"},"PeriodicalIF":1.4,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13537767/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148880547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vitamin K1 in indigenous vegetables from upper northern Thailand: High-performance liquid chromatography measurement and scenario-based intake assessment relevant to warfarin dietary counseling.","authors":"Chumaphorn Rodseeda, Nakit Chansamut","doi":"10.4103/JAPTR.JAPTR_42_26","DOIUrl":"10.4103/JAPTR.JAPTR_42_26","url":null,"abstract":"<p><p>Vitamin K1 intake is an important modifiable factor in dietary counseling for warfarin patients; however, region-specific food composition data in Thailand are limited. This small compositional pilot study aimed to determine Vitamin K1 concentrations in five commonly consumed indigenous vegetables from Upper Northern Thailand and to summarize estimated Vitamin K1 intake under predefined portion-size scenarios. Fresh vegetable samples were collected from local markets in Phayao Province and analyzed by the high-performance liquid chromatography with fluorescence detection. Estimated Vitamin K1 intake was modeled for three consumption scenarios (100, 300, and 1200 g/day) and compared to a benchmark intake of 150 μg/day from the literature. The concentration of Vitamin K1 ranged from 71.3 to 212.5 μg/100 g fresh weight, with <i>Gymnema inodorum</i> having the highest concentration. The 100 and 300 g/day scenarios were used as primary descriptive scenarios, whereas the 1200 g/day scenario was retained only as a conservative sensitivity analysis. These findings should be interpreted as descriptive intake estimates relevant to dietary counseling and not as evidence of altered anticoagulation outcomes. Data on the Vitamin K1 composition of different regions may help to provide more locally adapted dietary advice for patients taking warfarin.</p>","PeriodicalId":14877,"journal":{"name":"Journal of Advanced Pharmaceutical Technology & Research","volume":"17 3","pages":"208-212"},"PeriodicalIF":1.4,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13537792/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148880558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erratum: Evaluation of the combination of chitosan hydrogel crosslinker and dental pulp stem cell-derived exosomes on osteoblast viability and differentiation.","authors":"","doi":"10.4103/JAPTR.JAPTR_222_26","DOIUrl":"10.4103/JAPTR.JAPTR_222_26","url":null,"abstract":"<p><p>[This corrects the article on p. 191 in vol. 17, PMID: 42293278.].</p>","PeriodicalId":14877,"journal":{"name":"Journal of Advanced Pharmaceutical Technology & Research","volume":"17 3","pages":"316"},"PeriodicalIF":1.4,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13537788/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148880515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanovesicle-formulated <i>Sterculia coccinea</i> Jack (hantap) leaf extract: Estrogen receptor-alpha molecular docking and cytotoxicity in Michigan Cancer Foundation-7 cells.","authors":"Yuliet Susanto, Khildah Khaerati, Nela Sharon, Recky Patala, James Amedeus Mozart Sangtutu","doi":"10.4103/JAPTR.JAPTR_345_25","DOIUrl":"10.4103/JAPTR.JAPTR_345_25","url":null,"abstract":"<p><p><i>Sterculia coccinea</i> Jack (hantap) is an Indonesian medicinal plant with limited evidence of anticancer activity. The objective of this study was to identify the phytochemical components of hantap leaf extract, evaluate its interaction with estrogen receptor-alpha (ER-α) through molecular docking, and develop nanoliposome formulations to improve its physicochemical properties. The extract yield was 15.7%, and the phytochemical profile was characterized using liquid chromatography-high-resolution mass spectrometry. Molecular docking against ER-α was performed using tamoxifen as the reference ligand. Nanoliposomes were prepared by thin-film hydration using phosphatidylcholine and cholesterol at ratios of 0.6:0.3 (F1), 0.9:0.3 (F2), and 1.2:0.3 (F3). The formulations showed particle sizes of 158.3 ± 1.59 nm, 148.23 ± 2.68 nm, and 157.3 ± 1.10 nm; zeta potentials of - 30.16 ± 1.40 mV, -31.61 ± 0.16 mV, and - 32.84 ± 0.31 mV; and polydispersity index values of 0.28 ± 0.02, 0.29 ± 0.03, and 0.23 ± 0.02 for F1, F2, and F3, respectively. Entrapment efficiency for total phenolics ranged from 94.43% to 97.04%, while total flavonoid entrapment ranged from 32.84% to 73.54%. Docking analysis showed that linarin and apigenin had stronger binding affinity to ER-α than tamoxifen. Based on physicochemical evaluation, F2 was selected as the optimal formulation and showed weak cytotoxic activity against Michigan Cancer Foundation-7 (MCF-7) cells in the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assay, with an IC₅₀ value of 282.06 ± 3.39 μg/mL. In conclusion, hantap leaf extract nanoliposomes exhibited favorable nanoscale characteristics, while their cytotoxic effect against MCF-7 cells remained weak under the tested conditions.</p>","PeriodicalId":14877,"journal":{"name":"Journal of Advanced Pharmaceutical Technology & Research","volume":"17 3","pages":"241-248"},"PeriodicalIF":1.4,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13537785/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148880542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined inulin, glucomannan, and medium-chain triglyceride modulate metabolic and inflammatory responses in chemotherapy-treated colorectal cancer cells.","authors":"Bernadette Dian Novita, Yudy Tjahjono, Martha Ervina, Suryo Kuncorojakti, Andi Yasmin Wijaya, I Gede Putu Adhi Wedharga, Hendy Wijaya, Sianty Dewi, Herjuniato, Felix Christ Antono, Cornelia Valerie, Hevi Wihadmadyatami","doi":"10.4103/JAPTR.JAPTR_39_26","DOIUrl":"10.4103/JAPTR.JAPTR_39_26","url":null,"abstract":"<p><p>Colorectal cancer (CRC) remains a global health challenge. The efficacy of 5-fluorouracil (5-FU) is frequently limited by chemoresistance, inflammation, and compensatory pro-angiogenic signaling. Prebiotic fibers and metabolically active lipids have been shown to modulate immune-metabolic pathways that may influence tumor behavior and chemotherapy response. The aim of the study was to evaluate the immunomodulatory and chemo-adjunct effects of inulin, glucomannan, and a combined inulin-glucomannan-medium-chain triglyceride (MCT) formulation on CRC cells exposed to 5-FU. An <i>in vitro</i> experimental study using human colorectal adenocarcinoma HT-29 cells. HT-29 cells were exposed to graded concentrations of inulin, glucomannan, or their combination with MCT, either as single treatments or combined with 5-FU. Outcomes included MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)-based viability, clonogenic survival, and vascular endothelial growth factor (VEGF) and interleukin-6 (IL-6) expression using immunocytochemical (ICC). Data differences were analyzed using one-way analysis of variance with Tukey's <i>post hoc</i> test or the Kruskal-Wallis test with Dunn's <i>post hoc</i> test, as appropriate. Inulin increased short-term metabolic activity and partially attenuated 5-FU-induced cytotoxicity, whereas glucomannan demonstrated minimal effects. MCT supplementation further enhanced metabolic activity, indicating differential nutrient-chemotherapy interactions. The combined inulin-MCT-glucomannan formulation exerted the most consistent long-term effects, significantly reducing colony formation and modulating VEGF and IL-6 expression in a dose-dependent manner. However, VEGF increased at higher concentrations. The triple-component formulation modulated proliferative capacity and inflammatory-angiogenic signaling and altered cellular responses to 5-FU, supporting a modulatory rather than uniformly sensitizing role in CRC cells.</p>","PeriodicalId":14877,"journal":{"name":"Journal of Advanced Pharmaceutical Technology & Research","volume":"17 3","pages":"309-315"},"PeriodicalIF":1.4,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13537780/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148880530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined inulin and glucomannan modulate colonic cytokine balance and mucosal barrier function in a western diet-induced colonic inflammation mouse model.","authors":"Bernadette Dian Novita, Steven Christian Angkawijaya, I Gede Putu Adhi Wedharga, Suciatuz Zinanir Rohman, Ferdinand Wiliam Jaya, Daniella Widarma, David Timothy Abraham, Irene Lingkan Parengkuan, Adi Pramono Hendrata, Hendy Wijaya, Yudy Tjahjono, Sianty Dewi, Niluh Suwasanti, Endang Isbandiati Soediono, Suryo Kuncorojakti","doi":"10.4103/JAPTR.JAPTR_36_26","DOIUrl":"10.4103/JAPTR.JAPTR_36_26","url":null,"abstract":"<p><p>A rising worldwide health concern, inflammatory bowel disease is linked to western-style diets that damage intestinal barrier integrity, change gut microbiota, and increase colonic inflammation. In contrast, inulin and glucomannan enhance short-chain fatty acid production and preserve mucosal structure, suggesting a protective role against diet-induced colonic inflammation. This study aimed to investigate whether combined inulin and glucomannan supplementation prevents western diet-induced colonic inflammation by modulating intestinal barrier function and colonic inflammatory cytokine profiles. Over 8 weeks, elicited by a high-fat, high-sucrose (HFHS) diet, colonic inflammation in mice as a model was utilized in a preventative experimental investigation. Mice received an HFHS diet with supplementation of inulin, glucomannan, or their combination. Colonic histopathology was evaluated, and interleukin 6 (IL-6) and IL-10 levels were assessed using immunohistochemistry. Statistical analyses included one-way analysis of variance with appropriate <i>post hoc</i> tests, covariate-adjusted analyses controlling for caloric intake, and correlation analyses between histological and inflammatory parameters. Fiber supplementation reduced HFHS-induced colonic inflammation severity, crypt shortening, and mucosal thinning without affecting caloric intake. While colonic inflammation severity linked favorably with IL-6 and inversely with IL-10 and mucosal integrity, combined inulin-glucomannan supplementation provided the lowest IL-6/IL-10 ratio along with uniquely elevated IL-10 expression, indicating better immunomodulatory effects. Soluble fiber supplementation prevents HFHS-induced colonic inflammation by preserving mucosal architecture and restoring inflammatory cytokine balance, with combined inulin and glucomannan providing additive immunoprotective benefits.</p>","PeriodicalId":14877,"journal":{"name":"Journal of Advanced Pharmaceutical Technology & Research","volume":"17 3","pages":"221-226"},"PeriodicalIF":1.4,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13537796/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148880434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmacognostic profiling of <i>Bridelia ovata</i> leaves and evaluation of signal transducer and activator of transcription 3-mediated antiproliferative effects in triple-negative breast cancer.","authors":"Siti Nurfitriani Mandalika, Kasuni Gayathri Ileperuma, Krittapat Phairoh, Thanundorn Thanusuwannasak, Nonthaneth Nalinratana, Worathat Thitikornpong","doi":"10.4103/JAPTR.JAPTR_52_26","DOIUrl":"10.4103/JAPTR.JAPTR_52_26","url":null,"abstract":"<p><p><i>Bridelia ovata</i> Decne. is a Thai traditional medicinal plant whose pharmacognostic standards, validated analytical methods, and anticancer mechanisms against triple-negative breast cancer (TNBC) remain unreported. The objective of this study was to establish a pharmacognostic monograph for <i>B. ovata</i> leaves, validate a high performance liquid chromatography (HPLC) method for epigallocatechin (EGC) quantification, profile the extract by liquid chromatography/quadrupole time-of-flight mass spectrometry (LC-QTOF-MS), and investigate signal transducer and activator of transcription 3 (STAT3)-mediated anti-proliferative mechanisms in MDA-MB-231 TNBC cells. One authenticated and 14 commercial <i>B. ovata</i> leaf samples were evaluated by macroscopic and microscopic examination, thin-layer chromatography fingerprinting, phytochemical screening, physicochemical analysis, validated HPLC, LC-QTOF-MS profiling, network pharmacology, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, flow cytometry, and Western blotting. Key diagnostic features included paracytic stomata, lignified fibers, and calcium oxalate crystals. Most physicochemical parameters met World Health Organization general guidelines. The HPLC method showed excellent linearity (<i>r</i> ² > 0.9995; 20-500 μg/mL); EGC content ranged from 0.20 to 11.05% w/w. LC-QTOF-MS tentatively identified 24 metabolites spanning phenolic acids, catechins, flavonoid glycosides, and terpenoids. Furthermore, network pharmacology identified STAT3 as the key hub target. <i>B. ovata</i> extract selectively inhibited MDA-MB-231 viability (IC₅₀ = 413 ± 23 μg/mL vs. IC₅₀ > 800 μg/mL in MCF-10A), induced G2/M arrest, and suppressed p-STAT3 (Tyr705) and cyclin D1 expression in a dose-dependent manner. These findings integrate pharmacognostic identification, physicochemical standardization, and mechanistic evidence of STAT3 pathway modulation, collectively supporting <i>B. ovata</i> as a phytopharmaceutical candidate for TNBC. <i>B. ovata</i> leaves demonstrate reproducible pharmacognostic identity, consistent EGC-based chemical quality markers, and STAT3-mediated antiproliferative activity, warranting further preclinical investigation.</p>","PeriodicalId":14877,"journal":{"name":"Journal of Advanced Pharmaceutical Technology & Research","volume":"17 3","pages":"296-303"},"PeriodicalIF":1.4,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13537793/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148880485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characteristics of natural sunscreen cream-containing lignosulfonate, olive leaf extract, and propolis.","authors":"Dewi Sondari, Melati Septiyanti, Witta Kartika Restu, Yulianti Sampora, Rizna Triana Dewi, Rita Kartika Sari, Lilis Nurhasanah, Arzqa Sabila Hanifah, Zelda Aldilah Andari, Eka Novriyanti, Kanti Dewi Rizqiani","doi":"10.4103/JAPTR.JAPTR_330_25","DOIUrl":"10.4103/JAPTR.JAPTR_330_25","url":null,"abstract":"<p><p>A natural O/W sunscreen based on lignosulfonate, olive leaf extract, and propolis was developed. Formulations were evaluated for physicochemical properties, antioxidant and antibacterial activities, and sun protection factor (SPF). Results showed physical stability over 28 days with particle sizes of 2-28.53 μm and viscosities of 28-51 poise. The L3E3P10 formulation exhibited the highest SPF of 34.32. This phenolic combination synergistically enhances photoprotection.</p>","PeriodicalId":14877,"journal":{"name":"Journal of Advanced Pharmaceutical Technology & Research","volume":"17 3","pages":"256-269"},"PeriodicalIF":1.4,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13541350/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148891443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}