Journal of Advanced Pharmaceutical Technology & Research最新文献_第2页

Antifungal activities and in silico prediction of Moringa oleifera Lam. fractions of leaves against Candida albicans. 辣木抗真菌活性及其计算机预测。叶子的部分抗白色念珠菌。

IF 1.4

Journal of Advanced Pharmaceutical Technology & Research Pub Date : 2026-07-01 Epub Date: 2026-08-12 DOI: 10.4103/JAPTR.JAPTR_35_26

Ratna K Indrastiti, Febrina Rahmayanti, Dewi Fatma Suniarti, Dikdik Kurnia

{"title":"Antifungal activities and in silico prediction of Moringa oleifera Lam. fractions of leaves against Candida albicans.","authors":"Ratna K Indrastiti, Febrina Rahmayanti, Dewi Fatma Suniarti, Dikdik Kurnia","doi":"10.4103/JAPTR.JAPTR_35_26","DOIUrl":"10.4103/JAPTR.JAPTR_35_26","url":null,"abstract":"There is an escalating demand to identify novel bioactive compounds from medicinal plants due to the increasing number of antifungal-resistant pathogenic microorganisms. This study aimed to evaluate the antifungal activities of Moringa oleifera Lam. (MoLm.) leaves and the potential of its chemical constituents against Candida albicans, by inhibiting lanosterol-14-α-demethylase and (1,3)-β-D-glucan synthase, via molecular docking. This was an in vitro and in silico study. n-hexane, ethyl acetate, and methanol fractions were obtained from MoLm. leaves, with concentrations ranging from 10% to 50% (w/v) used to determine the zone of inhibition (ZoI), minimum inhibitory concentrations (MICs), and minimum fungicidal concentrations (MFCs). C. albicans strain ATCC 10231 was used. Three hundred chemical constituents were obtained from MoLm. fractions were obtained through liquid chromatography-mass spectrometry analysis, and echinenone, L-α-palmitin, betaine, α-linolenic acid, 2-pyrrolidone, curcumene, cinnamic acid, and methyl cinnamate were docked to lanosterol-14-α-demethylase and (1,3)-β-D-glucan synthase. The largest ZoI was observed in 10% ethyl acetate and 20% methanol fractions at 11.5 and 9 mm, respectively. The MICs of all fractions were within the range of 2.5%-5%, while only the n-hexane fraction showed an MFC of 10%. Echinenone had the strongest binding energy toward the enzymes, at -13.35 and -10.57 kcal/mol, and had the best Ki value of 1.65 × 10-4 and 1.8 × 10-2 μM, respectively. The docking results indicate that the chemical constituents were derived from MoLm. leaf fractions exhibit strong binding affinity toward critical molecular targets in C. albicans, suggesting their potential to disrupt essential cellular processes and contribute to antifungal activity.","PeriodicalId":14877,"journal":{"name":"Journal of Advanced Pharmaceutical Technology & Research","volume":"17 3","pages":"227-234"},"PeriodicalIF":1.4,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13537771/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148880389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Kaempferia galanga cream accelerates wound healing through modulation of tumor necrosis factor-alpha and interleukin-10 in an excision wound model. 在切除伤口模型中，山奈良膏通过调节肿瘤坏死因子- α和白细胞介素-10加速伤口愈合。

IF 1.4

Journal of Advanced Pharmaceutical Technology & Research Pub Date : 2026-07-01 Epub Date: 2026-08-12 DOI: 10.4103/JAPTR.JAPTR_18_26

Siska Siska, Fujianti Fujianti, Tahyatul Bariroh, Supandi Supandi, Islamudin Ahmad

{"title":"Kaempferia galanga cream accelerates wound healing through modulation of tumor necrosis factor-alpha and interleukin-10 in an excision wound model.","authors":"Siska Siska, Fujianti Fujianti, Tahyatul Bariroh, Supandi Supandi, Islamudin Ahmad","doi":"10.4103/JAPTR.JAPTR_18_26","DOIUrl":"10.4103/JAPTR.JAPTR_18_26","url":null,"abstract":"Traditionally used to treat inflammatory diseases, Kaempferia galanga L. includes bioactive substances with anti-inflammatory properties, such as eugenol and ethyl p-methoxycinnamate. Its potential as a topical treatment for wound healing is yet unclear, though. This study aims to assess the ability of K. galanga cream (KGC) in accelerating wound healing and to observe its effects on excisional wound models based on plasma levels of interleukin-10 (IL-10) and tumor necrosis factor-alpha (TNF-α). Six groups (n = 5) were created from thirty male Wistar rats. After creating a 1.5-cm full-thickness wound on the dorsal side, without treatment as a control, a base cream as a negative control, 10% povidone-iodine cream as a positive control, or KGC (5%, 10%, or 15%) was applied topically every day for 14 days. On days 7 and 14, the ImageJ software was used to quantify the wound area. Plasma TNF-α and IL-10 levels were measured on day 15 using enzyme-linked immunosorbent assay, and statistical evaluation was conducted via one-way analysis of variance with Tukey's post hoc test. KGC markedly improved wound healing versus the negative control. The 10% formulation produced the greatest effect, reducing wound area by 65.3% on day 14 (P < 0.001). It also markedly decreased TNF-α levels by 38.7% (P < 0.001) and increased IL-10 levels by 42.5% (P < 0.001) compared with the negative control. The 10% KGC demonstrated the most effective wound healing activity, likely through modulation of pro- and anti-inflammatory cytokines. These findings support its potential as a topical herbal formulation for wound management.","PeriodicalId":14877,"journal":{"name":"Journal of Advanced Pharmaceutical Technology & Research","volume":"17 3","pages":"282-287"},"PeriodicalIF":1.4,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13537770/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148880418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Calcium ionophore supplementation for immature oocytes and pentoxifylline supplementation for immotile sperms: A study of embryo quality of difficult cases in in vitro fertilization in a mouse model. 未成熟卵母细胞补充钙离子离子和不动精子补充己酮茶碱：小鼠体外受精困难病例胚胎质量的研究。

IF 1.4

Journal of Advanced Pharmaceutical Technology & Research Pub Date : 2026-07-01 Epub Date: 2026-08-12 DOI: 10.4103/JAPTR.JAPTR_44_26

Gito Restiansyah Wasian, Silvia Werdhy Lestari, Agustinus Agustinus, Ria Margiana, Ghayda Nafisa Assakura, Marieta Gladys Purwanto

{"title":"Calcium ionophore supplementation for immature oocytes and pentoxifylline supplementation for immotile sperms: A study of embryo quality of difficult cases in in vitro fertilization in a mouse model.","authors":"Gito Restiansyah Wasian, Silvia Werdhy Lestari, Agustinus Agustinus, Ria Margiana, Ghayda Nafisa Assakura, Marieta Gladys Purwanto","doi":"10.4103/JAPTR.JAPTR_44_26","DOIUrl":"10.4103/JAPTR.JAPTR_44_26","url":null,"abstract":"Difficult in vitro fertilization (IVF) cases involving both immature oocytes and immotile sperm remain a clinical challenge. This study evaluated embryo quality in a mouse model using pentoxifylline (PTX)-treated sperm and calcium ionophore (CaI)-matured oocytes. This was an experimental study using a mouse model. The immotile sperms were supplemented by PTX, while the immature oocytes were incubated with CaI. Then, the conventional IVF was performed to unite both germ cells. The development of the embryos was observed on days 1 and 3, respectively. Data were analyzed using SPSS. Oocyte maturity was compared between the groups using the Chi-square test. Sperm motility and embryo quality were analyzed using the Kruskal-Wallis test, followed by the Mann-Whitney post hoc test for pairwise comparisons. In this study, the maturation process was found more frequently with the addition of CaI (45.3%), and the motility of spermatozoa was also increased with the addition of PTX (31%). The quality of embryos after adding CaI and PTX (1st day - 58.3% and 3rd day - 33.3%) was significantly different from that without addition, although it was not different when compared to adding CaI alone or PTX alone. We conclude that in a mouse model, the addition of calcium for immature oocytes and PTX for immotile spermatozoa may assist in difficult cases in IVF practices, even though further research is needed, particularly in a clinical trial in human.","PeriodicalId":14877,"journal":{"name":"Journal of Advanced Pharmaceutical Technology & Research","volume":"17 3","pages":"203-207"},"PeriodicalIF":1.4,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13537794/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148880461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ameliorative effects of onion anthocyanin extract on spermatogenic function in cimetidine-induced testicular toxicity in male mice. 洋葱花青素提取物对西咪替定致雄性小鼠睾丸毒性致生精功能的改善作用。

IF 1.4

Journal of Advanced Pharmaceutical Technology & Research Pub Date : 2026-07-01 Epub Date: 2026-08-12 DOI: 10.4103/JAPTR.JAPTR_352_25

Farrah Rasool Jaafar, Shrooq J Jassim, Mohanad A Al-Bayati

{"title":"Ameliorative effects of onion anthocyanin extract on spermatogenic function in cimetidine-induced testicular toxicity in male mice.","authors":"Farrah Rasool Jaafar, Shrooq J Jassim, Mohanad A Al-Bayati","doi":"10.4103/JAPTR.JAPTR_352_25","DOIUrl":"10.4103/JAPTR.JAPTR_352_25","url":null,"abstract":"Cimetidine, an H2 receptor antagonist with anti-androgenic disrupts testicular structure and spermatogenesis. Anthocyanins in red onion (Allium cepa) have good antioxidant and cytoprotective action. This study compared the protective ability of onion-derived anthocyanin extract on the cimetidine-induced testicular toxicity in male mice, with respect to spermatogenic integrity, endocrine regulation, histopathology, and pharmacodynamic dose-response relationships. Sixty-four adult albino mice (male) were placed in control, anthocyanin (25-300 mg/kg), cimetidine (20 mg/kg), and cimetidine + anthocyanin (combinations) groups. Treatments were done in the form of intraperitoneally during 38 days. The assessment was done on testicular histology, seminiferous tubular diameter, germinal epithelium thickness, spermatogenic cell populations, Johnsen scores, daily sperm production, and Leydig cell indices. Anthocyanin co-treatment (25-300 mg/kg) produced a dose-dependent protective effect, with near-normal histological structure observed at 250-300 mg/kg. Daily sperm production increased significantly, showing a leftward shift in dose-response (ED50: 45.7 mg/kg vs. 57.9 mg/kg for anthocyanin alone). Onion anthocyanins exert potent, dose-dependent protective effects against cimetidine-induced testicular toxicity by preserving spermatogenesis and endocrine homeostasis, supporting their translational potential as fertility-protective adjuncts during gonadotoxic drug exposure.","PeriodicalId":14877,"journal":{"name":"Journal of Advanced Pharmaceutical Technology & Research","volume":"17 3","pages":"235-240"},"PeriodicalIF":1.4,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13537789/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148880447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel approach for wound treatment using Mitragyna speciosa-encapsulated nanostructured lipid carriers in hydrogel film. 水凝胶膜包裹纳米结构脂质载体的伤口治疗新方法。

IF 1.4

Journal of Advanced Pharmaceutical Technology & Research Pub Date : 2026-07-01 Epub Date: 2026-08-12 DOI: 10.4103/JAPTR.JAPTR_257_25

Anan Khadijah Imani Zulkifli, Siti Efliza Ashari, Syahida Ahmad, Masriana Hassan, Razana Mohd Ali

{"title":"A novel approach for wound treatment using Mitragyna speciosa-encapsulated nanostructured lipid carriers in hydrogel film.","authors":"Anan Khadijah Imani Zulkifli, Siti Efliza Ashari, Syahida Ahmad, Masriana Hassan, Razana Mohd Ali","doi":"10.4103/JAPTR.JAPTR_257_25","DOIUrl":"10.4103/JAPTR.JAPTR_257_25","url":null,"abstract":"Adequate wound healing is important for re-establishing the normal skin function. Determining the most suitable therapeutic strategy for skin repair involves multiple considerations, including wound type and condition, injury severity, lesion extent, and exudate level. Mitragyna speciosa (M. speciosa) is prominent for its therapeutic benefits and wound-healing properties. Nanostructured lipid carriers (NLCs) have proven beneficial for the M. speciosa extract, due to the restricted biocompatibility of active compounds in M. speciosa leaves. This study is the first to showcase the potential of M. speciosa leaf extract encapsulated in NLCs in a hydrogel for wound healing in rat models. M. speciosa leaf extract-encapsulated NLCs (MS-NLC) in hydrogel film can enhance wound recovery in rat models by facilitating faster wound contraction and promoting tissue repair in a shorter period as new growth tissue was visible at the peripheries of the abrasion observed from Day 3, hydroxyproline levels on day 21 was significantly higher than day 6 and day 15 (P > 0.05), and the reading of tumor growth factor-beta 1 (TGF-β1) through quantitative-polymerase chain reaction results on day 6 further revealed upregulation particularly in the 1000 mg/mL hydrogel group, reflecting active fibroblast function and tissue repair. TGF-β1 signaling is involved in key wound healing processes and could facilitate wound recovery observed with the MS-NLC hydrogel film, supporting its potential as a novel wound healing approach. However, the absence of a vehicle control limits the ability to attribute the observed effects solely to the active extract.","PeriodicalId":14877,"journal":{"name":"Journal of Advanced Pharmaceutical Technology & Research","volume":"17 3","pages":"275-281"},"PeriodicalIF":1.4,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13537777/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148880471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanostructured lipid carrier system encapsulating bergamot essential oil within an ionic gel-virgin coconut oil matrix: A promising approach for skin health care. 纳米结构脂质载体系统封装佛手柑精油在离子凝胶初榨椰子油基质：皮肤保健的一个有前途的方法。

IF 1.4

Journal of Advanced Pharmaceutical Technology & Research Pub Date : 2026-07-01 Epub Date: 2026-08-12 DOI: 10.4103/JAPTR.JAPTR_320_25

Arman Suryani, Rofi'uddin Ahmad, Tiaz Safitri, Lutfi Chabib, Erza Ridha Kartika

{"title":"Nanostructured lipid carrier system encapsulating bergamot essential oil within an ionic gel-virgin coconut oil matrix: A promising approach for skin health care.","authors":"Arman Suryani, Rofi'uddin Ahmad, Tiaz Safitri, Lutfi Chabib, Erza Ridha Kartika","doi":"10.4103/JAPTR.JAPTR_320_25","DOIUrl":"10.4103/JAPTR.JAPTR_320_25","url":null,"abstract":"Skin aging is a biological process, characterized by wrinkle formation, loss of elasticity, and decreased hydration. It is influenced by intrinsic and extrinsic factors, including ultraviolet (UV) exposure, pollution, and oxidative stress. This study developed a topical formulation combining bergamot essential oil (EOBs) and virgin coconut oil within an ionic gel system, encapsulated in nanostructured lipid carriers (NLCs), aiming to provide anti-aging, moisturizing, and UV-protective benefits. The GEOBs-VNLCs ionic gel formulation was prepared using a gelation method that combined sonication with dropwise stirring, followed by encapsulation within an NLC matrix via heating and homogenization, involving the formation of lipid and aqueous phases. The GEOBs-VNLCs formulation exhibited an average particle size of 140 ± 1.79 nm, low polydispersity (0.24 ± 0.02), negative zeta potential (-31.93 ± 1.17 mV), skin-compatible pH (5.13 ± 0.12), and high encapsulation efficiency (69.20 ± 0.57%), indicating good physicochemical stability for topical application. The formulation demonstrated moderate antioxidant activity (Half-maximal inhibitory concentration 7.39 μg/mL; 0.288 mg AAE/g dw), negligible improvement in skin hydration (P > 0.05), and low photoprotective performance (sun protection factor 8.12; PA+). The GEOBs-VNLCs formulation shows potential as a multifunctional, natural-based skincare system with antioxidant, moisturizing, and mild photoprotective properties.","PeriodicalId":14877,"journal":{"name":"Journal of Advanced Pharmaceutical Technology & Research","volume":"17 3","pages":"260-267"},"PeriodicalIF":1.4,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13537800/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148880507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fourier-transform infrared fingerprinting with chemometrics characterizes the anti-inflammatory activity of Rhizophora mucronata leaf fractions and profiles the active-fraction metabolites using liquid chromatography-high-resolution mass spectrometry analysis. 傅里叶变换红外指纹图谱与化学计量学表征了根参叶部分的抗炎活性，并利用液相色谱-高分辨率质谱分析分析了活性部分的代谢物。

IF 1.4

Journal of Advanced Pharmaceutical Technology & Research Pub Date : 2026-07-01 Epub Date: 2026-08-12 DOI: 10.4103/JAPTR.JAPTR_341_25

Lina Permatasari, Annisa Rizka Nirmala, Handa Muliasari

{"title":"Fourier-transform infrared fingerprinting with chemometrics characterizes the anti-inflammatory activity of Rhizophora mucronata leaf fractions and profiles the active-fraction metabolites using liquid chromatography-high-resolution mass spectrometry analysis.","authors":"Lina Permatasari, Annisa Rizka Nirmala, Handa Muliasari","doi":"10.4103/JAPTR.JAPTR_341_25","DOIUrl":"10.4103/JAPTR.JAPTR_341_25","url":null,"abstract":"The extract of Rhizophora mucronata leaves has demonstrated potential anti-inflammatory activity. Its fractions may exhibit enhanced activity due to the presence of more specific metabolites. This research was performed to determine the anti-inflammatory activity and characterize the R. mucronata leaf fractions using Fourier-transform infrared (FTIR) spectroscopy integrated with chemometric analysis. This was an experimental in vitro study evaluating the anti-inflammatory activity of R. mucronata leaf fractions and characterizing their chemical profiles using FTIR and liquid chromatography-high-resolution mass spectrometry (LC-HRMS). The ethanol extract was fractionated using vacuum liquid chromatography. The anti-inflammatory activity was evaluated by measuring the inhibition of egg albumin denaturation in the sample. The fractions were characterized using FTIR spectroscopy combined with chemometrics. The secondary metabolite profile of the fraction with the highest anti-inflammatory activity was examined using LC-HRMS. The FTIR profile of R. mucronata leaves was analyzed using principal component analysis (PCA) and hierarchical cluster analysis. Fraction N has the highest percentage of egg albumin denaturation inhibition activity. Naringin and ferulic acid were the most abundant flavonoid and phenolic compounds detected in fraction N. FTIR spectra indicated variations in chemical composition among fractions. In the PCA biplot, fraction N was closely aligned with the variable at 1376 cm-1, indicating strong C-H bending of methyl groups. Fraction N demonstrated the ability to inhibit egg albumin denaturation. Nevertheless, further investigation is necessary to confirm these results.","PeriodicalId":14877,"journal":{"name":"Journal of Advanced Pharmaceutical Technology & Research","volume":"17 3","pages":"249-255"},"PeriodicalIF":1.4,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13537799/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148880512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of yogurt and ultra-high-temperature milk on serum osteocalcin and calcium levels in rats. 酸奶和超高温牛奶对大鼠血清骨钙素和钙水平的影响。

IF 1.4

Journal of Advanced Pharmaceutical Technology & Research Pub Date : 2026-07-01 Epub Date: 2026-08-12 DOI: 10.4103/JAPTR.JAPTR_56_26

Renata Junilla, Raden Andri Primadhi, Muhamad Naseh Sajadi Budi Irawan

{"title":"Comparison of yogurt and ultra-high-temperature milk on serum osteocalcin and calcium levels in rats.","authors":"Renata Junilla, Raden Andri Primadhi, Muhamad Naseh Sajadi Budi Irawan","doi":"10.4103/JAPTR.JAPTR_56_26","DOIUrl":"10.4103/JAPTR.JAPTR_56_26","url":null,"abstract":"Dairy products may influence bone metabolism through calcium bioavailability and modulation of gut microbiota. This study compared the effects of yogurt and ultra-high-temperature (UHT) milk on serum osteocalcin and calcium levels in rats. Twenty-four male Wistar rats (Rattus norvegicus) were randomly assigned to control, UHT milk, and yogurt groups (n = 8 each) and administered the intervention for 8 weeks. Serum osteocalcin was measured at weeks 4 and 8, and serum calcium was measured at week 8. At week 4, both UHT milk and yogurt groups demonstrated significantly higher osteocalcin levels than the control group. A significant difference was also observed between the UHT milk and yogurt groups (P = 0.046). At week 8, osteocalcin levels remained significantly higher in both intervention groups than in the control group (P < 0.001), whereas no significant difference was detected between the UHT milk and yogurt groups (P = 0.674). Serum calcium levels were also significantly higher in the intervention groups than in the control group (P < 0.001), with higher levels observed in the yogurt group than in the UHT milk group (P = 0.037). Yogurt and UHT milk were associated with increased serum osteocalcin and calcium levels in rats. Yogurt was associated with earlier increases in circulating osteocalcin levels and higher serum calcium levels, whereas both dairy products demonstrated comparable osteocalcin levels after 8 weeks. These findings reflect changes in circulating biochemical markers associated with bone metabolism rather than direct evidence of enhanced bone formation or skeletal structural improvement.","PeriodicalId":14877,"journal":{"name":"Journal of Advanced Pharmaceutical Technology & Research","volume":"17 3","pages":"304-308"},"PeriodicalIF":1.4,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13537787/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148880564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Saurauia vulcani leaf extract on spleen morphometric and histopathological changes in rats infected with Salmonella typhimurium. 野樱叶提取物对鼠伤寒沙门菌感染大鼠脾脏形态学及组织病理学改变的影响。

IF 1.4

Journal of Advanced Pharmaceutical Technology & Research Pub Date : 2026-04-01 Epub Date: 2026-05-09 DOI: 10.4103/JAPTR.JAPTR_339_25

Samuel Manutur Simanjuntak, Evita Mayasari, Lokot Donna Lubis, Sana Noreen, Yunita Sari Pane

{"title":"Effect of Saurauia vulcani leaf extract on spleen morphometric and histopathological changes in rats infected with Salmonella typhimurium.","authors":"Samuel Manutur Simanjuntak, Evita Mayasari, Lokot Donna Lubis, Sana Noreen, Yunita Sari Pane","doi":"10.4103/JAPTR.JAPTR_339_25","DOIUrl":"10.4103/JAPTR.JAPTR_339_25","url":null,"abstract":"Typhoid-like systemic infection caused by Salmonella species can induce immune responses affecting lymphoid organs such as the spleen. Saurauia vulcani (pirdot) is a traditional medicinal plant known to contain bioactive compounds with potential immunomodulatory properties. This study aimed to evaluate the effect of S. vulcani leaf extract on spleen morphometry and histopathology in rats infected with Salmonella typhimurium. Thirty-two male Wistar rats were randomly divided into four groups (n = 8): normal control (K1), negative control infected with S. typhimurium (K2), positive control treated with chloramphenicol (K3), and treatment group receiving S. vulcani extract (K4). The extract was administered orally at a dose of 500 mg/kg body weight. Spleen weight, spleen volume (cm3), and histological features were evaluated. Data were analyzed using nonparametric tests and presented as medians. Infection increased spleen weight and volume compared to the normal control. The treatment group showed a decreasing trend in spleen weight and volume compared to the negative control; however, the differences were not statistically significant (P > 0.05). Histological observations indicated improved preservation of splenic architecture in the treatment group compared to infected controls. S. vulcani leaf extract showed a tendency to maintain spleen morphology during S. typhimurium infection; however, no statistically significant effect was observed.","PeriodicalId":14877,"journal":{"name":"Journal of Advanced Pharmaceutical Technology & Research","volume":"17 2","pages":"179-183"},"PeriodicalIF":1.4,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13262919/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148251685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the combination of chitosan hydrogel crosslinker and dental pulp stem cell-derived exosomes on osteoblast viability and differentiation. 壳聚糖水凝胶交联剂与牙髓干细胞外泌体结合对成骨细胞活力和分化的影响。

IF 1.4

Journal of Advanced Pharmaceutical Technology & Research Pub Date : 2026-04-01 Epub Date: 2026-05-09 DOI: 10.4103/JAPTR.JAPTR_312_25

Endang Winiati Bachtiar, Irene Edith Rieuwpassa, Turmidzi Fath, Juni Handajani, Widya Lestari

{"title":"Evaluation of the combination of chitosan hydrogel crosslinker and dental pulp stem cell-derived exosomes on osteoblast viability and differentiation.","authors":"Endang Winiati Bachtiar, Irene Edith Rieuwpassa, Turmidzi Fath, Juni Handajani, Widya Lestari","doi":"10.4103/JAPTR.JAPTR_312_25","DOIUrl":"10.4103/JAPTR.JAPTR_312_25","url":null,"abstract":"Dental pulp stem cell (DPSC) has gained attention as a cell-free therapeutic approach to enhance bone regeneration. However, the degradation of exosomal biomaterials limits their application, which may be overcome by using an injectable chitosan hydrogel crosslinker (ICHC) as a natural polymeric carrier. ICHC was prepared and loaded with exosomes derived from DPSCs. Cytotoxicity, calcium deposition, nanoparticle tracking analysis (NTA), and transmission electron microscopy (TEM) were conducted to assess biocompatibility, mineralization capacity, and structural characteristics. The cell viability of ICHCs, exosome 5 ng/mL, and exosome-loaded ICHC 0.6% ranged from 95% to 132%. NTA analysis revealed particle sizes of 147.5 nm for exosomes and 377.5 nm for exosome-loaded ICHC, with concentrations of 4.6 × 106 and 1.4 × 107 particles/mL, respectively. TEM further validated effective encapsulation, revealing electron-dense particles dispersed throughout the ICHC hydrogel matrix. The combination of exosome-loaded ICHC achieved 100% viability in osteoblast cells and promoted more elongated cell morphology compared to the control, suggesting a safe and effective cell-free material for bone regeneration therapy.","PeriodicalId":14877,"journal":{"name":"Journal of Advanced Pharmaceutical Technology & Research","volume":"17 2","pages":"191-197"},"PeriodicalIF":1.4,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13262911/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148251672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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