Mycochemical analysis and antibacterial activities of aqueous Kulat Pelawan (Heimioporus spp.) mushroom extracts.

IF 1.4 Q3 Pharmacology, Toxicology and Pharmaceutics
Sari Darmasiwi, Lucia Dhiantika Witasari, Alivia Nur Maysaroh, Arfista Deska Khairunnisa
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引用次数: 0

Abstract

The bioprospection of Heimioporus spp. has not been extensively explored because of its limited distribution and cultivation. This investigation aims to examine the proximate, mycochemicals, and antibacterial properties of aqueous Heimioporus spp. extracts. The dried mushroom was analyzed for nutritional content quantitatively, and mycochemicals were identified quantitatively. Antimicrobial activities were studied using disc diffusion assays and minimum inhibitory and bactericidal concentrations. Mushroom extracts were produced by maceration at 25°C ± 2°C for 48 h and boiled at 100°C for 30 min to yield cold aqueous extract (CAE) and hot aqueous extract (HAE), respectively, which were then tested against Escherichia coli, Staphylococcus aureus, and Propionibacterium acnes. The mycochemicals were analyzed for their alkaloids, flavonoids, terpenoids, and saponins. The study utilized ANOVA and DMRT statistical analysis software, with P < 0.05, to analyze the results. Results showed high protein (30.88%), carbohydrate (47.57%), and fat content (0.61%). The CAE showed higher antibacterial activity than the HAE against E. coli (9.67 ± 0.58 mm) and S. aureus (10.00 ± 0.00 mm) but no antibacterial activity against P. acnes (P < 0.05). The CAE had more mycochemicals than HAE. Results suggested that Heimioporus spp. is a potential functional food with health benefiits, which has high lipid, carbohydrate, and protein content. Its extracts revealed strong antibacterial properties against E. coli and S. aureus but no inhibition against P. acnes.

白桦栗(Kulat Pelawan, Heimioporus spp.)菌液的真菌化学分析及抑菌活性。
由于黑孔菌属的分布和栽培范围有限,其生物前景尚未得到广泛探讨。本研究的目的是研究海蚤水提取物的近似值、霉菌化学物质和抗菌性能。对其营养成分进行了定量分析,并对真菌化学物质进行了定量鉴定。采用圆盘扩散法和最低抑菌浓度和杀菌浓度研究其抑菌活性。在25°C±2°C条件下浸泡48 h,在100°C条件下煮沸30 min,分别得到冷水浸提物(CAE)和热水浸提物(HAE),然后对大肠杆菌、金黄色葡萄球菌和痤疮丙酸杆菌进行抑菌试验。分析真菌化学物质的生物碱、黄酮类、萜类和皂苷。采用方差分析和DMRT统计分析软件对结果进行分析,P < 0.05。结果:蛋白质(30.88%)、碳水化合物(47.57%)、脂肪(0.61%)含量高。CAE对大肠杆菌(9.67±0.58 mm)和金黄色葡萄球菌(10.00±0.00 mm)的抑菌活性高于HAE,但对痤疮链球菌(P < 0.05)没有抑菌活性。CAE比HAE有更多的真菌化学物质。研究结果表明,海螺具有较高的脂质、碳水化合物和蛋白质含量,是一种具有潜在保健价值的功能性食品。其提取物对大肠杆菌和金黄色葡萄球菌有较强的抑菌作用,但对痤疮杆菌无抑制作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
2.00
自引率
7.10%
发文量
44
审稿时长
20 weeks
期刊介绍: Journal of Advanced Pharmaceutical Technology & Research (JAPTR) is an Official Publication of Society of Pharmaceutical Education & Research™. It is an international journal published Quarterly. Journal of Advanced Pharmaceutical Technology & Research (JAPTR) is available in online and print version. It is a peer reviewed journal aiming to communicate high quality original research work, reviews, short communications, case report, Ethics Forum, Education Forum and Letter to editor that contribute significantly to further the scientific knowledge related to the field of Pharmacy i.e. Pharmaceutics, Pharmacology, Pharmacognosy, Pharmaceutical Chemistry. Articles with timely interest and newer research concepts will be given more preference.
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