Investigative ophthalmology & visual science最新文献

{"title":"Epac1 Inhibits Orbital Fibroblast Activation to Ameliorate Thyroid-Associated Orbitopathy-Like Features Through the JAK/STAT Signaling Pathway.","authors":"Rong Liu, Xin Xin, Shunguo Ma, Jinmin Guo, Chenmian Wu, Xin Qi, Ban Luo","doi":"10.1167/iovs.66.9.68","DOIUrl":"https://doi.org/10.1167/iovs.66.9.68","url":null,"abstract":"<p><strong>Purpose: </strong>Orbital fibroblast (OF) activation plays an important role in thyroid-associated orbitopathy (TAO) development. Epac1, as a cyclic adenosine monophosphate (cAMP) effector, has been recognized as a pivotal mediator of the anti-fibrosis properties of cAMP. This study investigated the role and mechanisms of Epac1 in OF activation.</p><p><strong>Methods: </strong>Clinical orbital samples were collected from patients with TAO and normal healthy volunteers, and a TAO mouse model was established using adenovirus expressing the human TSHR A subunit (Ad-TSHR), which was evaluated for histopathological features and detected for Epac1 and vimentin expression levels. Clinical TAO samples- (TAO OFs) and healthy people-derived OFs (normal OFs) were isolated and identified using immunofluorescent (IF) staining. Healthy and TAO OFs were transfected with Epac1-overexpressing plasmid (Epac1) for Epac1 overexpression or sh-Epac1 plasmid for Epac1 knockdown, treated with TGFβ1, and then examined for cell phenotypes and the expression level of Epac1 and fibrosis-related markers (α-SMA, fibronectin, and collagens). Furthermore, adeno-associated virus (AAV) overexpressing Epac1 (AAV-Epac1) was injected into TAO mouse orbital tissues to investigate the anti-fibrotic effects of Epac1 on TGFβ1-stimulated OFs in vivo. Moreover, the phosphorylation of STAT3 in the TGFβ1-stimulated TAO OFs and TAO mice model was investigated. The JAK/STAT signaling inhibitor Stattic was used to explore the involvement of the JAK/STAT signaling in the functions of Epac1.</p><p><strong>Results: </strong>Both clinical TAO samples and the TAO mouse model demonstrated significantly changed histopathology, reduced Epac1 expression, and increased vimentin level. TGFβ1 stimulation significantly facilitated cell viability and migration of normal OFs and TAO OFs, and elevated α-SMA, fibronectin, vimentin, collagen I, and collagen III expression levels. However, Epac1 overexpression in OFs could notably attenuate these pro-fibrosis effects of TGFβ1 on both normal and TAO OFs, shown as inhibiting cell viability and migration and decreasing expression of fibrotic markers. Whereas, Epac1 knockdown aggravated TGFβ1-induced fibrosis. In vivo, Epac1 overexpression also improved TAO-like symptoms in TAO model mice. Mechanically, Epac1 overexpression inhibited STAT3 phosphorylation in vitro and in vivo. Stattic effectively attenuated the effects of Epac1 knockdown on TGFβ1-treated TAO OFs.</p><p><strong>Conclusions: </strong>Epac1 was a critical regulator of fibrotic and inflammatory processes in TAO through mediating the JAK/STAT signaling pathway.</p>","PeriodicalId":14620,"journal":{"name":"Investigative ophthalmology & visual science","volume":"66 9","pages":"68"},"PeriodicalIF":4.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144742078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erratum in: Childhood Myopia Part I: Contemporary Treatment Options.","authors":"","doi":"10.1167/iovs.66.9.46","DOIUrl":"10.1167/iovs.66.9.46","url":null,"abstract":"","PeriodicalId":14620,"journal":{"name":"Investigative ophthalmology & visual science","volume":"66 9","pages":"46"},"PeriodicalIF":5.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12279068/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144642508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mycobacterium tuberculosis Infection of Retinal Endothelial Cells Induces Interferon Signaling Activation: Insights Into Tubercular Retinal Vasculitis.","authors":"Ikhwanuliman Putera, Sigrid M A Swagemakers, Nicole M A Nagtzaam, Conny van Holten-Neelen, Rina La Distia Nora, Jurriaan E M de Steenwinkel, Saskia M Rombach, P Martin van Hagen, Willem A Dik","doi":"10.1167/iovs.66.9.48","DOIUrl":"10.1167/iovs.66.9.48","url":null,"abstract":"<p><strong>Purpose: </strong>Ocular tuberculosis (OTB) has diverse clinical presentations, among which choroidal granuloma and tubercular retinal vasculitis (TRV) are recognized as typical phenotypes. The potential role of human retinal endothelial cells (RECs) in regulating inflammation in response to Mycobacterium tuberculosis (Mtb) infection, particularly relevant in cases of TRV, remains elusive. This study investigated the cellular defense of Mtb-exposed RECs.</p><p><strong>Methods: </strong>Human RECs, as a TRV model, were exposed to either live H37Rv Mtb or heat-killed (HK)-H37Rv Mtb. Total RNA and culture supernatants were collected 24 hours post-exposure, and cellular responses were characterized using RNA sequencing and bioinformatics analysis.</p><p><strong>Results: </strong>Both live and HK-Mtb were internalized by RECs, but only live Mtb induced a robust transcriptional response, with 322 differentially expressed genes. Live Mtb infection, but not HK-Mtb exposure, induced a dominant interferon (IFN) signaling response, particularly type I IFN activation. This was validated by real-time polymerase chain reaction (RT-PCR) for 10 IFN-inducible genes. Network analysis suggested a role of RECs in leucocyte recruitment and activation. Although HK-Mtb increased the production of CCL2, IL-6, and IL-8, this was not observed with live Mtb infection. Instead, live Mtb infection led to elevated CXCL10 and IP-10 production.</p><p><strong>Conclusions: </strong>RECs elicit a robust immune response to Mtb infection. IFN signaling activation was observed in live Mtb-infected RECs but not in HK-Mtb-exposed RECs. These findings provide insights into TRV pathogenesis and offer clues for potential biomarkers that may help differentiate TRV caused by active infection, which requires antitubercular treatment, from cases without active infection, which require immunosuppressive therapy alone.</p>","PeriodicalId":14620,"journal":{"name":"Investigative ophthalmology & visual science","volume":"66 9","pages":"48"},"PeriodicalIF":5.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12279072/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144642510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Phenotypic Study of CRB1 Retinopathy Secondary to the Variant p.(Pro836Thr) Prevalent in Those of Black African Ancestry.","authors":"Wendy M Wong, Anthony G Robson, Rebecca A Baker, Gavin Arno, Joseph Van Aerschot, Siying Lin, Mariya Moosajee, Michel Michaelides, Omar A Mahroo, Andrew R Webster","doi":"10.1167/iovs.66.9.3","DOIUrl":"10.1167/iovs.66.9.3","url":null,"abstract":"<p><strong>Purpose: </strong>To comprehensively characterize the clinical consequences of the CRB1 variant p.(Pro836Thr). In African populations, this variant has an allele frequency of 0.329% (gnomAD v4.1.0).</p><p><strong>Methods: </strong>This study was a retrospective case series of 14 patients from 11 families with molecularly confirmed CRB1-associated retinal dystrophy, each possessing at least one p.(Pro836Thr) variant. The age at onset of visual symptoms, best-corrected visual acuity, imaging findings, and quantitative electrophysiologic measurements of retinal function were analyzed.</p><p><strong>Results: </strong>The p.(Pro836Thr) variant was homozygous in four families and compound heterozygous in seven families. The familial origins included Nigeria (n = 4), Ghana (n = 3), the Caribbean region (n = 2), and Uganda (n = 1). The median follow-up was 7 years (interquartile range, 3-16). Symptom onset was most common in childhood (eight patients, 57.1%). Reduced central vision was the most frequent presenting symptom (12 patients, 85%). Widefield multimodal imaging revealed peripheral retinal changes in addition to macular changes in three patients. Nine patients had international standard electrophysiology and showed generalized retinal dysfunction with a similar degree of rod and cone system involvement (n = 7) or a clear rod-cone pattern of dysfunction (n = 2). All had pattern electroretinography (ERG) evidence of macular dysfunction.</p><p><strong>Conclusions: </strong>The study highlights the association of the p.(Pro836Thr) variant with African ancestry and characterizes their key clinical and electrophysiological features. Our study suggests that the p.(Pro836Thr) variant confers a less severe consequence on retinal function and structure than the majority of other reported CRB1 variants. Although retinal imaging may show alterations confined to the macular region, electrophysiology in this series indicates generalized cone and rod photoreceptor dysfunction.</p>","PeriodicalId":14620,"journal":{"name":"Investigative ophthalmology & visual science","volume":"66 9","pages":"3"},"PeriodicalIF":5.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12227024/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144540229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erratum in: Assessment of Neuroprotective Effects of Glutamate Modulation on Glaucoma-Related Retinal Ganglion Cell Apoptosis In Vivo.","authors":"","doi":"10.1167/iovs.66.9.33","DOIUrl":"10.1167/iovs.66.9.33","url":null,"abstract":"","PeriodicalId":14620,"journal":{"name":"Investigative ophthalmology & visual science","volume":"66 9","pages":"33"},"PeriodicalIF":5.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12266285/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144608431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Perception of Straight Ahead With Vision, Hearing, and Proprioception by Individuals With Low Vision.","authors":"Diamond Brunt, Gordon E Legge, Donald C Fletcher, Yingzi Xiong","doi":"10.1167/iovs.66.9.38","DOIUrl":"10.1167/iovs.66.9.38","url":null,"abstract":"<p><strong>Purpose: </strong>The perception of straight ahead (PSA) is an important reference for spatial orientation. Although individuals with low vision often report difficulties in spatial tasks, it is not clear whether they face challenges in defining PSA. We asked whether low vision leads to increased biases and variabilities in visual PSA, and whether these changes, if any, would generalize to auditory PSA, due to the dominant role of vision in spatial tasks.</p><p><strong>Methods: </strong>Thirty-five low-vision participants and 17 age-matched control participants completed visual and auditory PSA tasks in which they adjusted the location of a brief LED light or a finger snap sound from a speaker to appear to be straight ahead. To control for proprioception, participants also completed a PSA task in which they pointed to their straight ahead while blindfolded.</p><p><strong>Results: </strong>The magnitudes of biases and variabilities in visual PSA were significantly associated with binocular acuity and contrast sensitivity, but the laterality of the visual biases was not associated with interocular asymmetries in acuity, contrast sensitivity, or lateral asymmetries in the binocular visual field. Low-vision individuals showed larger magnitudes of biases and variabilities in the auditory PSA independent of age or hearing status, possibly indicating a cross-modal impact of vision loss on sound localization. Despite the changes in visual and auditory PSA, the low-vision participants showed intact proprioception PSA.</p><p><strong>Conclusions: </strong>The changes in visual and auditory PSA may contribute to spatial orientation challenges encountered by low-vision individuals, and the robust proprioceptive PSA may play an important compensatory role.</p>","PeriodicalId":14620,"journal":{"name":"Investigative ophthalmology & visual science","volume":"66 9","pages":"38"},"PeriodicalIF":5.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12270021/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144626307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic Analysis of Human Lens Epithelium Tissue With and Without Cataract Surgery: Uncovering Novel Pathways of Post-Surgical Lens Epithelium Remodeling.","authors":"Caili Hao, Kailin Li, Zongbo Wei, Kazi Rafsan Radeen, Xiang Zhang, Sharad Purohit, Xingjun Fan","doi":"10.1167/iovs.66.9.28","DOIUrl":"10.1167/iovs.66.9.28","url":null,"abstract":"<p><strong>Purpose: </strong>Cataract surgery is the most commonly performed ophthalmic procedure worldwide, yet long-term molecular adaptations of lens epithelial cells (LECs) following surgery remain poorly understood.</p><p><strong>Methods: </strong>We conducted bulk RNA sequencing on lens capsules from 34 human donors, including 17 with an average of 7 years post-cataract surgery and 17 non-cataract controls.</p><p><strong>Results: </strong>Differential expression analysis revealed substantial transcriptional changes in post-surgical LECs, including 1690 upregulated and 579 downregulated genes. Gene Set Enrichment Analysis (GSEA) and Gene Ontology (GO) identified activation of oxidative stress response, cell survival, and immune response signatures. Notably, genes involved in antioxidant defense (e.g. SOD2, GCLC, and TXN), anti-apoptotic regulation (BCL2L1 and XIAP), and DNA repair (ERCC1) were significantly upregulated in post-surgical samples. Immune-related genes (IL-8, CCL2, and TGFβ2) and complement components (C2 and C3) were elevated, suggesting persistent inflammatory signaling. Enrichment of TGF-β and Wnt/β-Catenin signaling, along with increased expression of epithelial mesenchymal transition (EMT) and fibrosis markers (ACTA2, FN1, and TNC), indicated long-term fibrotic remodeling. Senescence-associated genes (CDKN2A and CDKN1A) were also upregulated, whereas LMNB1 was downregulated, supporting a senescent phenotype in a subset of LECs. Immunofluorescence confirmed senescence and fibrosis at the protein level. GO enrichment and clustering revealed strong upregulation of cell migration, corroborated by elevated expression of migratory genes (ICAM1, VCAM1, and PLAU), suggesting acquisition of an invasive phenotype.</p><p><strong>Conclusions: </strong>Our findings reveal that LECs undergo sustained molecular reprogramming after cataract surgery, including inflammatory, fibrotic, migratory, and senescent changes. These adaptations may contribute to posterior capsule opacification (PCO) and highlight therapeutic targets for its prevention.</p>","PeriodicalId":14620,"journal":{"name":"Investigative ophthalmology & visual science","volume":"66 9","pages":"28"},"PeriodicalIF":5.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12266286/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144608436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation of Multimodal Clinical Imaging With the Whole-Slide- and Superresolution-Based Immunohistological Structure of the Corneal Limbal Stroma.","authors":"Gréta Kemenes, István Rebenku, Beáta Bajdik, György Vereb, Lili Takács","doi":"10.1167/iovs.66.9.54","DOIUrl":"10.1167/iovs.66.9.54","url":null,"abstract":"<p><strong>Purpose: </strong>To identify structural landmarks in the limbal stroma by anterior segment optical coherence tomography (asOCT), asOCT angiography (asOCTa) and in vivo confocal microscopy (IVCM), and correlate findings with immunofluorescence microscopy (IFM).</p><p><strong>Methods: </strong>The corneal limbus of healthy individuals was examined by IVCM, asOCT, and asOCTa. IFM was performed in cadaveric cornea samples sectioned in clinical imaging planes, with putative markers for limbal niche cells (CD90, N-cadherin, SSEA-4), blood/lymphatic vessels (CD31), nerves (acetyl-α-tubulin, β-III tubulin), limbal epithelial progenitor cells (p63), and hyaluronic acid (HA).</p><p><strong>Results: </strong>On asOCT, asOCTa, and IVCM superficial and deep limbal vessels could be identified that terminated in the marginal corneal arcade (MCA). Limbal stroma appeared as hyperreflective area between superficial and deep limbal blood vessels. The same blood vessel patterns were identified by IFM. HA localization was identical to hyperreflective stromal structures. Nerves and putative niche cells localized around limbal vessels and were in close contact with the basal limbal epithelium at the level of the MCA. Limbal epithelium was hyperreflective and thinned in the elderly (>60 years, 122.6 ± 36.6 µm; <60 years, 139.9 ± 34.4 µm; P = 0.025) resulting in less visible palisades of Vogt on asOCT (>60 years, 82.6% visible; <60 years, 100% visible; P = 0.011).</p><p><strong>Conclusions: </strong>Our study proves that limbal vessel plexuses can serve as landmarks to identify corresponding structures in various clinical imaging modalities. The proximity of blood vessels, niche cells, and nerves, confirmed by IFM, may suggest that limbal vascular damage occurs together with niche cell and neural loss. The IFM correlations provided by this study help to detect healthy limbal structures and aid the diagnosis of diseased corneas.</p>","PeriodicalId":14620,"journal":{"name":"Investigative ophthalmology & visual science","volume":"66 9","pages":"54"},"PeriodicalIF":4.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12302048/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144674798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deep Learning Model for Automated Classification of Macular Neovascularization Subtypes in AMD.","authors":"Giovanni Neri, Chiara Rebecchi, Jonathan D Oakley, Chiara Olivieri, Federico Ricardi, Paola Marolo, Daniel B Russakoff, Michele Reibaldi, Enrico Borrelli","doi":"10.1167/iovs.66.9.55","DOIUrl":"10.1167/iovs.66.9.55","url":null,"abstract":"<p><strong>Purpose: </strong>To develop a deep learning algorithm capable of accurately classifying macular neovascularization (MNV) subtypes in patients with treatment-naïve exudative neovascular age-related macular degeneration (AMD) using structural optical coherence tomography (OCT) images.</p><p><strong>Methods: </strong>In this retrospective cohort study, a total of 193 eyes with treatment-naïve neovascular AMD were included. Each case was classified into MNV subtypes (type 1, 2, or 3) based on structural OCT features. Convolutional neural network (CNN)-based deep learning models were trained using cross-validation to classify MNV subtypes. Preprocessing included homogenization of image data to optimize use of layer information for classification. Performance metrics included sensitivity, specificity, and area under the receiver operating characteristic (ROC) curve (AUC), with and without homogenization.</p><p><strong>Results: </strong>Homogenized OCT data improved classification performance compared to non-homogenized data for all models. The highest reported sensitivity and specificity for type 1 MNV was 96.7% and 84.9%; for type 2, 100.0% and 85.5%; and, for type 3, 84.9% and 87.9%, respectively. The AUCs for type 1, 2, and 3 MNV were 0.95, 0.97, and 0.91, respectively. Occlusion sensitivity analysis revealed critical regions for classification, highlighting distinct anatomical differences among MNV subtypes.</p><p><strong>Conclusions: </strong>The proposed deep learning model demonstrated high accuracy in classifying MNV subtypes on structural OCT, with improved performance following homogenization. This tool could assist clinicians in accurately and efficiently diagnosing MNV subtypes, potentially influencing treatment decisions and patient outcomes in neovascular AMD.</p>","PeriodicalId":14620,"journal":{"name":"Investigative ophthalmology & visual science","volume":"66 9","pages":"55"},"PeriodicalIF":4.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12302047/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144674799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early Photoreceptor Alterations After Retinal Detachment Repair.","authors":"Sandrine H Sassen, Josua Sassen, Marlene Sassmannshausen, Lukas Goerdt, Yannick Liermann, Raffael G Liegl, Philipp Herrmann, Robert P Finger, Frank G Holz, Sarah Thiele","doi":"10.1167/iovs.66.9.32","DOIUrl":"10.1167/iovs.66.9.32","url":null,"abstract":"<p><strong>Purpose: </strong>The importance of outer retinal microstructure, specifically the external limiting membrane (ELM) and ellipsoid zone (EZ), was recently highlighted for visual recovery after rhegmatogenous retinal detachment (RRD). Quantitative assessment of the EZ/ELM reflectivity, termed the relative EZ reflectivity (rEZR), is a novel SD-OCT imaging biomarker for photoreceptor integrity. This study evaluates the rEZR's functional relevance after anatomically successful RRD repair, as well as its association with the surgical techniques.</p><p><strong>Methods: </strong>Patients with primary RRD, treated at the Department of Ophthalmology, University of Bonn/Germany between April 2021 and April 2023 and presenting to the six-week postoperative review with complete retinal reattachment, were identified. The rEZR (arbitrary units [AU]) was determined in the postoperative imaging data. Linear-mixed effect models, corrected for patient's age and preoperative macula-on/macula-off RRD situation, were applied to assess the association of the rEZR with the postoperative best-corrected visual acuity (BCVA) and with the previously applied surgical techniques.</p><p><strong>Results: </strong>A total of 187 patients with a median [interquartile range (IQR)] age of 70.0 [9.7] years (32.1% female) were included. Out of those, 91.4% (n = 171) underwent vitrectomy and 8.6% scleral buckling. Higher rEZR values were significantly associated with a better BCVA (coefficient estimate [CE] = 0.3009 [0.1620; 0.4398], P < 0.001) and scleral buckling (CE = 12.20 [0.33; 24.07], P < 0.044).</p><p><strong>Conclusions: </strong>This study supports the functional relevance of outer retinal impairment, as quantified by rEZR, and its association with surgery-related variables in the early postoperative period after RRD repair. Further research is needed to better understand the importance of longitudinal rEZR changes to finally achieve the best visual outcomes for RRD patients.</p>","PeriodicalId":14620,"journal":{"name":"Investigative ophthalmology & visual science","volume":"66 9","pages":"32"},"PeriodicalIF":5.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12266289/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144608374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}