Investigative ophthalmology & visual science最新文献

{"title":"Changes in Lens Thickness and Power Before and After Myopia Onset.","authors":"Jiaqing Zhang, Ling Jin, Qianyun Chen, Decai Wang, Xiang Chen, Yuting Li, Yabin Qu, Rong Lin, Mingguang He, Ian G Morgan, Lixia Luo, Yangfa Zeng, Xiaotong Han","doi":"10.1167/iovs.66.3.36","DOIUrl":"10.1167/iovs.66.3.36","url":null,"abstract":"<p><strong>Purpose: </strong>To investigate changes in the crystalline lens before and after the onset of myopia.</p><p><strong>Methods: </strong>This prospective cohort study was conducted in Guangzhou, Guangdong, China. Participants were initially emmetropic or hyperopic at baseline and were followed for 5 years, during which they were categorized into two groups: those who developed myopia (newly developed myopes [NDM]; n = 1669) and those who remained hyperopic or emmetropic (persistent nonmyopes [PNM]; n = 4259). Changes in spherical equivalent refraction (SER), axial length, lens thickness, and lens power were analyzed from 5 years before to 4 years after myopia onset. Age-related trends in SER and biometric parameters were compared between the PNM and NDM groups.</p><p><strong>Results: </strong>The mean age of the included children at baseline was 7.61 ± 2.68 years (range, 3-14 years), with 3272 boys (55.20%). Compared with the PNM group, the NDM group exhibited a faster myopic refraction shift, accelerated axial elongation, and a more rapid reduction in lens thickness and power. Changes in SER and axial length peaked at 1 year before myopia onset (P < 0.001), but lens thickness and power remained relatively stable before myopia onset. The rate of change in SER and biometric parameters all slowed after myopia onset. In the PNM group, lens thickness decreased before age 11 and increased thereafter, whereas lens power decreased continuously, with a slower rate of decline after 11 years of age.</p><p><strong>Conclusions: </strong>SER and axial length demonstrate accelerated changes 1 year before myopia onset, whereas lens thickness and power remain largely stable before and after myopia onset, with changes primarily associated with age.</p>","PeriodicalId":14620,"journal":{"name":"Investigative ophthalmology & visual science","volume":"66 3","pages":"36"},"PeriodicalIF":5.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11927298/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143656987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Perceptual Visual Acuity Declines With Age in a Rat Model of Retinitis Pigmentosa While Light Perception is Maintained.","authors":"Naofumi Suematsu, Akinori Y Sato, Akihiro Kimura, Satoshi Shimegi, Shogo Soma","doi":"10.1167/iovs.66.3.31","DOIUrl":"10.1167/iovs.66.3.31","url":null,"abstract":"<p><strong>Purpose: </strong>Retinitis pigmentosa (RP) is a leading cause of blindness and genetically induces impairment of the retinal epithelium and photoreceptors. In this study, we investigated the decline in the visual response and visual ability during disease progression. This understanding is crucial for disease staging in patients, establishing therapeutic plans in advance, and evaluating the effects of interventional treatments.</p><p><strong>Methods: </strong>We used a rat model of inherited RP (Royal College of Surgeons [RCS] rats) and evaluated form visual acuity and light perception using behavioral tests and electrophysiological recordings in the dorsal lateral geniculate nucleus, superior colliculus, and primary visual cortex.</p><p><strong>Results: </strong>The perceptual form vision (detection of grating stimulus) was attenuated by 9 weeks old. The neural responses in the three early visual areas to flashing grating stimuli with various contrasts and spatial frequencies showed similar degeneration progress as the behavioral evaluations. Light perception (detection of a bright uniform light source) was maintained until at least 11 weeks old. The neural responses to the uniform flashlight stimulus in the three early visual areas were maintained during the same period.</p><p><strong>Conclusions: </strong>Our findings suggest that form vision is primarily affected by the progression of RP, whereas non-form vision is potentially robust to retinal degeneration. This maintenance of light perception is likely due to the preserved function of intrinsically photosensitive retinal ganglion cells. These results provide useful and fundamental knowledge for evaluating the protective or restorative effects of experimental treatments for RP.</p>","PeriodicalId":14620,"journal":{"name":"Investigative ophthalmology & visual science","volume":"66 3","pages":"31"},"PeriodicalIF":5.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11925224/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143647995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characteristics of Retina, Choroid, and Retrobulbar Blood Flow in Myopes With Posterior Staphyloma Using Ultra-Widefield OCTA and CDI.","authors":"Fang Liu, Yunzhe Wang, Lingling Niu, Jing Zhao, Kang Xue, Xingtao Zhou","doi":"10.1167/iovs.66.3.21","DOIUrl":"10.1167/iovs.66.3.21","url":null,"abstract":"<p><strong>Purpose: </strong>To explore the characteristics of retinal, choroidal, and retrobulbar blood flows in myopic patients with posterior staphyloma (PS) using ultra-widefield optical coherence tomography angiography (UWF-OCTA) and Color Doppler imaging (CDI).</p><p><strong>Methods: </strong>The retrospective cross-sectional study enrolled 134 adults with high myopia from the Eye and ENT Hospital of Fudan University from December 2021 to September 2022. After propensity score matching, 45 eyes of 30 patients and 45 eyes of 32 patients were included in the PS and non-PS (NPS) groups, respectively. Retinal, choroidal, and retrobulbar blood flow parameters were obtained from UWF-OCTA and CDI. Parameters were compared between the PS and NPS groups, as well as among different PS types.</p><p><strong>Results: </strong>There were no statistical differences in age, sex, axial length, spherical equivalent, intraocular pressure, or best-corrected visual acuity between the PS and NPS groups or in the different types of PS. The PS group had lower fovea, parafovea, and perifovea choroidal vessel volume (CVV) compared to the NPS group (all P < 0.05). The wide and narrow macular types of PS had significantly thinner choroidal thickness (CHT), lower CVV, and lower choroidal stromal volume (CSV) than other groups (all P < 0.01). PS occurrence was correlated with the decrease in CVV and posterior ciliary artery end-diastolic velocity (PCA-EDV), and the decrease in PCA-EDV was an associated factor for PS. The wide and narrow macular types of PS were correlated with the decrease of CHT, CVV, and CSV; the decrease in perifovea CHT was an associated factor for wide and narrow macular types of PS.</p><p><strong>Conclusions: </strong>Change in blood flow of the PCA was associated with PS occurrence. Reduced CHT in the temporal perifoveal region was a factor associated with both wide and narrow macular types of PS. Further longitudinal study will help to investigate the causal relationship between PS and changes in blood flow.</p>","PeriodicalId":14620,"journal":{"name":"Investigative ophthalmology & visual science","volume":"66 3","pages":"21"},"PeriodicalIF":5.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11905596/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143585651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distribution of Globe Excursions Within the Orbits Monitored by Eye Tracking Glasses in Ambulatory Subjects Engaged in Their Normal Daily Activities.","authors":"Yicen J Zheng, Thomas N Gentry, John R Economides, Jonathan C Horton","doi":"10.1167/iovs.66.3.20","DOIUrl":"10.1167/iovs.66.3.20","url":null,"abstract":"<p><strong>Purpose: </strong>It is unknown how gaze angle deviates over the course of normal daily activities, and whether its distribution is affected by vergence. To address these issues, an eye tracker was used to record eye positions in ambulatory subjects engaged in their usual pursuits.</p><p><strong>Methods: </strong>Twenty-seven normal subjects with a mean age of 23.6 years (range, 4-68 years) wore the eye tracking glasses, generating 328 min/person of usable data. Histograms were compiled to show the distribution of (1) horizontal gaze angles, (2) vertical gaze angles, and (3) vergence.</p><p><strong>Results: </strong>The histogram of horizontal gaze angles showed a bimodal distribution of vergence, with a distance peak at 1.6° and a near peak at 7.6°. The mean standard deviation of eye position was greater during far viewing (8.93°) than near viewing (6.65°). Horizontal eye position deviated by more than 25° from primary position less than 1% of the time. Vertical eye position shifted from a mean of -3.58° during far viewing to -8.54° during near viewing. Overall, the standard deviation of vertical eye position (11.63°) was greater than that of horizonal eye position (8.41°).</p><p><strong>Conclusions: </strong>Deployment of a mobile eye tracker has revealed four main findings about eye position in the orbit. First, horizontal gaze angle remains within ±16.8° of primary gaze 95% of the time, reflecting an aversion to large horizontal ocular excursions. Second, mean vertical gaze position is shifted downward (-5.19°). Third, increased vergence is associated with a gaze shift downward. Fourth, increased vergence reduces the distribution of horizontal eye positions.</p>","PeriodicalId":14620,"journal":{"name":"Investigative ophthalmology & visual science","volume":"66 3","pages":"20"},"PeriodicalIF":5.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11905580/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143585695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Epidemiology of Eye Cancer, Eyelid Cancer, and Ophthalmic Lymphoma in a Chinese Population in Hong Kong: A Population-Based Registry Study 2005-2018.","authors":"Allie Lee, Dennis T W Law, Alex H S Kan, Flores F P Cruz, Vishal Jhanji, Hunter K L Yuen, Michael Y Ni","doi":"10.1167/iovs.66.3.15","DOIUrl":"10.1167/iovs.66.3.15","url":null,"abstract":"<p><strong>Purpose: </strong>To investigate the incidence rate, histopathological types, and time trends of primary eye cancer, eyelid cancer, and ophthalmic lymphoma among the Chinese population.</p><p><strong>Methods: </strong>The records of patients diagnosed with primary eye and eyelid cancers from 2005 to 2018 in Hong Kong were retrieved from the Hong Kong Cancer Registry. Patient demographics, cancer sites, and histology were recorded. Population-level age-standardized incidences and trends were analyzed by age and sex and compared with existing literature. International Classification of Diseases, Ninth Edition (ICD-9) codes were converted to ICD-10 codes. Eye cancer was defined as malignancy involving the eyeball, intraocular structures, lacrimal system, and orbit.</p><p><strong>Results: </strong>A total of 442 eye cancers and 1103 eyelid cancers were included. Among these cancers, 295 cases were ophthalmic lymphoma. The age-standardized annual incidence rate was 4.04 per 1,000,000 population for eye cancer, 6.30 for eyelid cancer, and 1.89 for ophthalmic lymphoma. The incidence rates increased with age (P < 0.001), but there were no sex differences. The incidence rate of eyelid cancer and ophthalmic lymphoma showed an increasing trend, with annual percent changes of 2.8% and 2%, respectively. In eye cancers, the most common histological type was lymphoma (58.1%), and the most common site was orbit (35.5%). The most common histology for eyelid cancer was basal cell carcinoma, and for ophthalmic lymphoma it was extranodal marginal zone B cell lymphoma.</p><p><strong>Conclusions: </strong>In the Chinese population, the incidence of eye cancer has remained stable, whereas eyelid cancer and ophthalmic lymphoma are increasing. Lymphoma has been the most common histological type in recent years, in contrast to findings in Western populations.</p>","PeriodicalId":14620,"journal":{"name":"Investigative ophthalmology & visual science","volume":"66 3","pages":"15"},"PeriodicalIF":5.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11895851/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143567137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modulation of All-Trans Retinoic Acid by Light and Dopamine in the Murine Eye.","authors":"Sarah Talwar, Reece Mazade, Melissa Bentley-Ford, Jianshi Yu, Nageswara Pilli, Maureen A Kane, C Ross Ethier, Machelle T Pardue","doi":"10.1167/iovs.66.3.37","DOIUrl":"10.1167/iovs.66.3.37","url":null,"abstract":"<p><strong>Purpose: </strong>Ambient light exposure is linked to myopia development in children and affects myopia susceptibility in animal models. Currently, it is unclear which signals mediate the effects of light on myopia. All-trans retinoic acid (atRA) and dopamine (DA) oppositely influence experimental myopia and may be involved in the retinoscleral signaling cascade underlying myopic eye growth. However, how ocular atRA responds to different lighting and whether atRA and DA interact remains unknown.</p><p><strong>Methods: </strong>Dark-adapted C57BL/6J mice (29-31 days old) were exposed to dim (1 lux), mid (59 lux), or bright (12,000 lux) ambient lighting for 5 to 60 minutes. Some mice were also systemically administered the DA precursor, LDOPA, or atRA before light exposure. After exposure, the retina and the back of the eye (BOE) were collected and analyzed for levels of atRA, DA, and the DA metabolite, DOPAC.</p><p><strong>Results: </strong>DA turnover (DOPAC/DA ratio) in the retina increased in magnitude after only 5 minutes of exposure to higher ambient luminance, but was minimal in the BOE. In contrast, atRA levels in the retina and BOE significantly decreased with higher ambient luminance and longer duration exposure. Intriguingly, LDOPA-treated mice had a transient reduction in retinal atRA compared with saline-treated mice, whereas atRA treatment had no effect on ocular DA.</p><p><strong>Conclusions: </strong>Ocular atRA was affected by the duration of exposure to different ambient lighting, and retinal atRA levels decreased with increased DA. Overall, these data suggest specific interactions between ambient lighting, atRA, and DA that could have implications for the retinoscleral signaling cascade underlying myopic eye growth.</p>","PeriodicalId":14620,"journal":{"name":"Investigative ophthalmology & visual science","volume":"66 3","pages":"37"},"PeriodicalIF":5.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11927300/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143656990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Value of MLPA for Prognostic Assessment of Chromosomal Rearrangements and DNA Methylation in Uveal Melanoma.","authors":"Andrea Soltysova, Dana Dvorska, Andrej Ficek, Martina Pecimonova, Marek Samec, Ivana Kasubova, Viera Horvathova Kajabova, Lucia Demkova, Pavel Babal, Jela Valaskova, Zuzana Dankova, Bozena Smolkova, Alena Furdova","doi":"10.1167/iovs.66.3.51","DOIUrl":"10.1167/iovs.66.3.51","url":null,"abstract":"<p><strong>Purpose: </strong>Uveal melanoma (UM) is the most prevalent primary intraocular malignancy in adults, with prognosis significantly influenced by genetic and epigenetic factors. Reliable and cost-effective methods to detect chromosomal aberrations and DNA methylation changes are essential for improving prognostication and informing treatment strategies in UM. This study evaluated the effectiveness of multiplex ligation-dependent probe amplification (MLPA) in detecting UM-specific copy number variations (CNVs) and promoter methylation changes across 25 tumor suppressor genes (TSGs).</p><p><strong>Methods: </strong>DNA from 58 UM tissues was analyzed with the SALSA MLPA Probemix P027 Uveal melanoma kit, and a subset of 18 samples was further assessed using the SALSA MLPA Probemix ME002-C1 Tumour suppressor mix 2 kit to identify key CNVs and methylation alterations linked to poor prognosis. Validation was carried out with a high-resolution comparative genomic hybridization (CGH) array on 10 samples and the Illumina Infinium Methylation EPIC v1.0 BeadChip array on 25 samples.</p><p><strong>Results: </strong>Our findings indicate that MLPA is a versatile and robust method for detecting CNVs, showing strong correlations with CGH data and highlighting specific CNV patterns linked to clinical outcomes in UM. However, the ME002-C1 kit showed limited utility for comprehensive methylation analysis, as differential methylation was not observed in the studied TSG loci.</p><p><strong>Conclusions: </strong>Although MLPA effectively identifies CNVs relevant to UM prognosis, integrating additional methylation-specific approaches could broaden the scope of DNA methylation analysis, offering a more comprehensive molecular understanding of UM that may enhance prognostication and personalized treatment.</p>","PeriodicalId":14620,"journal":{"name":"Investigative ophthalmology & visual science","volume":"66 3","pages":"51"},"PeriodicalIF":5.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11951064/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143700293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Age-Related Changes in Mesopic Reading Vision Across Adulthood.","authors":"Boris Peñaloza, Traci-Lin Goddin, David S Friedman, Cynthia Owsley, MiYoung Kwon","doi":"10.1167/iovs.66.3.40","DOIUrl":"10.1167/iovs.66.3.40","url":null,"abstract":"<p><strong>Purpose: </strong>Reading is indispensable for daily activities such as reading books, menus, and food labels, occurring under a wide range of luminance conditions from mesopic (dim light) to photopic (daylight). Despite its significance, there has been limited attention on age-related changes in mesopic reading vision. The current study aims to investigate how mesopic reading vision changes across adulthood.</p><p><strong>Methods: </strong>Using the MNREAD iPad app, we assessed both mesopic (2 cd/m2) and photopic (220 cd/m2) reading vision in 157 normally-sighted individuals aged from 18 to 84, grouped into seven age groups. Reading vision was evaluated using four MNREAD parameters: maximum reading speed (MRS), critical print size (CPS), reading acuity (RA), and reading accessibility index (ACC).</p><p><strong>Results: </strong>There was a significant age-related decline in reading vision under both mesopic and photopic conditions, with a more pronounced decline observed in mesopic conditions. The decline was linear from age 20 to 80: MRS decreased by 30 words-per-minute in mesopic conditions and 29 words-per-minute in photopic conditions; ACC declined by 0.18 (mesopic) and 0.12 (photopic); CPS declined by 0.3 logMAR (mesopic) and 0.16 logMAR (photopic); RA declined by 0.24 logMAR (mesopic) and 0.18 logMAR (photopic).</p><p><strong>Conclusions: </strong>Our results show a monotonic decline in reading vision from ages 20 to 80 under both mesopic and photopic conditions, with a more pronounced decline in mesopic light. Given the significance of reading vision as a clinical measure, assessing reading vision under mesopic conditions may offer a more comprehensive evaluation of functional vision in everyday life.</p>","PeriodicalId":14620,"journal":{"name":"Investigative ophthalmology & visual science","volume":"66 3","pages":"40"},"PeriodicalIF":5.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11932422/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143663354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Partial Sleep Deprivation on Retinal Microvasculature in Myopia With Different Axial Lengths.","authors":"Ke Xu, Yishuang Xu, Youran Qin, Yufei Zhang, Hongmei Zheng, Changzheng Chen, Yu Su","doi":"10.1167/iovs.66.3.57","DOIUrl":"10.1167/iovs.66.3.57","url":null,"abstract":"<p><strong>Purpose: </strong>To investigate the effects of partial sleep deprivation (PSD) on the retinal microvasculature in individuals with myopia, using optical coherence tomography angiography (OCTA).</p><p><strong>Methods: </strong>In total, 87 right eyes were categorized by axial length (AL) into three groups: A (AL < 24 mm, n = 20), B (24 ≤ AL < 26 mm, n = 41), and C (AL ≥ 26 mm, n = 26). Participants underwent macular (6 mm × 6 mm) and optic disc (4.5 mm × 4.5 mm) OCTA scans. Vessel density (VD) parameters-including superficial capillary plexus VD (SCP-VD), deep capillary plexus VD (DCP-VD), radial peripapillary capillary VD (RPC-VD), inside disc RPC VD (iVD), and peripapillary RPC VD (ppVD)-were compared at baseline, before and after PSD, and following 3 days of regular sleep.</p><p><strong>Results: </strong>Compared with group A, groups B and C had lower baseline DCP-VD in all subregions (P < 0.05), except for the perifovea-inferior area, where only groups C and A showed significant differences (P = 0.001). After PSD, group C showed a decrease in SCP-VD in certain subregions of the parafovea (P = 0.013 and P = 0.022 for parafovea-temporal and parafovea-inferior, respectively), along with an increase in ppVD (P = 0.012). All VD parameters recovered after 3 days of regular sleep (P < 0.05).</p><p><strong>Conclusions: </strong>The retinal microvasculature of myopic eyes with an AL of ≥26 mm show greater susceptibility to PSD than those with an AL of <26 mm. However, short-term PSD effects can be restored by setting a regular sleep schedule.</p>","PeriodicalId":14620,"journal":{"name":"Investigative ophthalmology & visual science","volume":"66 3","pages":"57"},"PeriodicalIF":5.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11954537/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143719374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fibronectin Mediates Endothelial-to-Mesenchymal Transition in Retina Angiogenesis.","authors":"Dan Liu, Zhishang Meng, Chen Jin, Fang Chen, Li Pu, Ze Wu, Qi Zeng, Jing Luo, Wenyi Wu","doi":"10.1167/iovs.66.3.10","DOIUrl":"10.1167/iovs.66.3.10","url":null,"abstract":"<p><strong>Purpose: </strong>The purpose of this study was to investigate the role of endothelial-mesenchymal transition (EndoMT) in pathological retinal angiogenesis and identify key molecular mediators in retina angiogenesis.</p><p><strong>Methods: </strong>RNA sequencing (RNA-seq) was performed on retinal tissue from an oxygen-induced retinopathy (OIR) mouse model to analyze gene expression patterns. The Gene Set Enrichment Analysis was used to examine the correlation between epithelial-mesenchymal transition (EMT) and angiogenesis gene sets. Fibronectin (FN1) expression was evaluated in endothelial cells, and its function was assessed through siRNA-mediated knockdown in both in vitro angiogenesis assays and the OIR model.</p><p><strong>Results: </strong>EndoMT occurred early in retinal angiogenesis development, with significant correlation between EMT and angiogenesis gene sets. FN1 was identified as the most significantly upregulated EMT-related gene in endothelial cells. The siRNA-mediated inhibition of fibronectin effectively prevented VEGF-induced angiogenesis in vitro and reduced pathological angiogenesis in the OIR model.</p><p><strong>Conclusions: </strong>EndoMT is a crucial early event in pathological retinal angiogenesis, with fibronectin serving as a key mediator. Targeting fibronectin may provide a novel therapeutic strategy that could synergize with anti-VEGF treatments to more effectively treat pathological angiogenesis in diabetic retinopathy (DR) and retinopathy of prematurity (ROP), particularly in cases of poor response to anti-VEGF therapy alone.</p>","PeriodicalId":14620,"journal":{"name":"Investigative ophthalmology & visual science","volume":"66 3","pages":"10"},"PeriodicalIF":5.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11892531/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143556672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}