Japanese journal of infectious diseases最新文献_第5页

Surveillance of SARS-CoV-2 Infection in Rodent Populations in Tokyo, Japan. 日本东京啮齿动物群体中 SARS-CoV-2 感染的监测。

IF 1.3 4区医学

Japanese journal of infectious diseases Pub Date : 2024-11-21 Epub Date: 2024-07-31 DOI: 10.7883/yoken.JJID.2024.143

Yudai Kuroda, Akitoyo Hotta, Masakatsu Taira, Nobuo Koizumi, Kango Tatemoto, Eun-Sil Park, Milagros Virhuez-Mendoza, Tsukasa Yamamoto, Noriyuki Komatsu, Keigo Shibo, Takeshi Sasaki, Yoshiyuki Mori, Kentaro Watanabe, Yasushi Kiyokawa, Yuya Eguchi, Azusa Banzai, Hirotaka Katahira, Tsutomu Tanikawa, Ken Maeda

{"title":"Surveillance of SARS-CoV-2 Infection in Rodent Populations in Tokyo, Japan.","authors":"Yudai Kuroda, Akitoyo Hotta, Masakatsu Taira, Nobuo Koizumi, Kango Tatemoto, Eun-Sil Park, Milagros Virhuez-Mendoza, Tsukasa Yamamoto, Noriyuki Komatsu, Keigo Shibo, Takeshi Sasaki, Yoshiyuki Mori, Kentaro Watanabe, Yasushi Kiyokawa, Yuya Eguchi, Azusa Banzai, Hirotaka Katahira, Tsutomu Tanikawa, Ken Maeda","doi":"10.7883/yoken.JJID.2024.143","DOIUrl":"10.7883/yoken.JJID.2024.143","url":null,"abstract":"The persistence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) among wildlife populations poses a potential risk for the emergence of novel variants. Therefore, monitoring SARS-CoV-2 infections in animals is crucial. As urban rodents live in close proximity to human habitats, there is a concern that they may be a potential source of zoonoses. To examine the prevalence of SARS-CoV-2 in rodent populations, we analyzed 128 serum samples and 129 oral swabs collected from 128 brown rats (Rattus norvegicus) and two black rats (Rattus rattus) captured for pest control purposes in Tokyo, Japan, between May and December 2023. A virus-neutralizing test using the omicron variant revealed no evidence of SARS-CoV-2 infection. Real-time reverse transcription- PCR of oral swabs did not detect any SARS-CoV-2 RNA-positive rats. These results indicate a low probability of SARS-CoV-2 circulation among rat populations in Tokyo.","PeriodicalId":14608,"journal":{"name":"Japanese journal of infectious diseases","volume":" ","pages":"342-344"},"PeriodicalIF":1.3,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141859790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic Characteristics and Phylogenetic Analysis of Coxsackievirus A6 Isolated in Linyi, China, 2022-2023. 2022-2023 年在中国临沂分离的柯萨奇病毒 A6 的遗传特征和系统发育分析。

IF 1.3 4区医学

Japanese journal of infectious diseases Pub Date : 2024-11-21 Epub Date: 2024-06-28 DOI: 10.7883/yoken.JJID.2024.072

Jie Chen, Pengfei You, Xiaoyan Chen, Huafeng Li, Na Zhang, Guangyun Zhang, Conghong Xu, Chunling Ma, Yanli Zhang, Tiegang Lv

{"title":"Genetic Characteristics and Phylogenetic Analysis of Coxsackievirus A6 Isolated in Linyi, China, 2022-2023.","authors":"Jie Chen, Pengfei You, Xiaoyan Chen, Huafeng Li, Na Zhang, Guangyun Zhang, Conghong Xu, Chunling Ma, Yanli Zhang, Tiegang Lv","doi":"10.7883/yoken.JJID.2024.072","DOIUrl":"10.7883/yoken.JJID.2024.072","url":null,"abstract":"Hand, foot, and mouth disease (HFMD) has become one of the most common infectious diseases in the past few decades. Since 2013, coxsackievirus A6 (CVA6) has replaced enterovirus A71 (EV-A71) and coxsackievirus A16 (CVA16), becoming the predominant pathogen responsible for HFMD in many areas of China. This study aimed to investigate the genetic characteristics and molecular epidemiology of CVA6 in Linyi between the years 2022 and 2023. In total, 965 patients with HFMD were enrolled in this study, and analyses based on VP1 nucleotide sequences were performed to determine the evolutionary trajectory of CVA6. In 2022, 281/386 (72.8%) patients were positive for enteroviruses (EVs) and 217/281 (77.2%) were CVA6 positive. In 2023, 398/579 (68.7%) samples were positive for EVs and 243/398 (61.1%) were CVA6 positive. Six sequences were selected each year for homology analysis. The results showed that the 12 strains isolated from Linyi were distant from the prototype strain (AY421764) and the first CVA6 strain reported in China (JQ364886). Phylogenetic analysis showed that the CVA6 strains isolated from Linyi belonged to the D3 sub-genotype. CVA6 is emerging as a common pathogen causing HFMD in Linyi and continuous surveillance of HFMD etiologies agents is necessary.","PeriodicalId":14608,"journal":{"name":"Japanese journal of infectious diseases","volume":" ","pages":"311-316"},"PeriodicalIF":1.3,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141467921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distribution and Antimicrobial Susceptibility Pattern of CTX-M-type Extended-Spectrum β-Lactamase-Producing Escherichia coli Isolated in Chubu Region, Japan. 日本中部地区分离到的产 CTX-M 型广谱 β-内酰胺酶大肠杆菌的分布和抗菌药敏感性模式。

IF 1.3 4区医学

Japanese journal of infectious diseases Pub Date : 2024-11-21 Epub Date: 2024-06-28 DOI: 10.7883/yoken.JJID.2024.079

Kazuya Itadani, Yoshimi Oonishi, Harumi Hisada, Tomoaki Tanaka, Shingo Mizunaga, Yuka Yamagishi, Hiroshige Mikamo

{"title":"Distribution and Antimicrobial Susceptibility Pattern of CTX-M-type Extended-Spectrum β-Lactamase-Producing Escherichia coli Isolated in Chubu Region, Japan.","authors":"Kazuya Itadani, Yoshimi Oonishi, Harumi Hisada, Tomoaki Tanaka, Shingo Mizunaga, Yuka Yamagishi, Hiroshige Mikamo","doi":"10.7883/yoken.JJID.2024.079","DOIUrl":"10.7883/yoken.JJID.2024.079","url":null,"abstract":"The widespread prevalence of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli limits treatment options and is a worldwide problem. This study aimed to investigate the antimicrobial susceptibility and ESBL types of 204 strains of CTX-M-type ESBLsproducing E. coli isolated from 2011 to 2017 in the Chubu region of Japan and to identify factors correlated with susceptibility. Minimal inhibitory concentrations were determined in accordance with the guidelines of the Clinical and Laboratory Standards Institute. Genes encoding β-lactamases were detected by PCR amplification. The CTX-M subtypes were determined using sequence analyses. CTX- M-15-producing strains showed significantly lower susceptibility rates to tazobactam/piperacillin (TAZ/ PIPC) than CTX-M-14 and -27-producing strains. Additional analyses of secondary β-lactamases revealed that most of the OXA-1-positive strains were CTX-M-15-producing strains (94.7%). The OXA-1-positive strains displayed significantly lower susceptibility to TAZ/PIPC (47.4%), sulbactam/ ampicillin (0.0%), and amikacin (73.7%) than the OXA-1-negative strains, suggesting that the high non-susceptibility rate of the CTX-M-15-producing strain was due to the co-carriage of OXA-1. Statistical analyses showed that OXA-1-positive strains were present in significant amounts in patients aged ≥65 years, suggesting that older patients have a higher risk of being refractory to treatment.","PeriodicalId":14608,"journal":{"name":"Japanese journal of infectious diseases","volume":" ","pages":"334-341"},"PeriodicalIF":1.3,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141467920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Case of Food Poisoning Caused by Campylobacter jejuni after the Ingestion of Undercooked Chicken Meal with Subsequent Development of Guillain-Barré Syndrome. 一例因摄入未煮熟鸡肉而导致空肠弯曲菌食物中毒并继发格林-巴利综合征的病例

IF 1.3 4区医学

Japanese journal of infectious diseases Pub Date : 2024-11-21 Epub Date: 2024-06-28 DOI: 10.7883/yoken.JJID.2024.108

Satoru Akase, Hiromi Obata, Wakaba Okada, Dai Saiki, Noriko Konishi, Keiko Yokoyama, Kenji Sadamasu

{"title":"A Case of Food Poisoning Caused by Campylobacter jejuni after the Ingestion of Undercooked Chicken Meal with Subsequent Development of Guillain-Barré Syndrome.","authors":"Satoru Akase, Hiromi Obata, Wakaba Okada, Dai Saiki, Noriko Konishi, Keiko Yokoyama, Kenji Sadamasu","doi":"10.7883/yoken.JJID.2024.108","DOIUrl":"10.7883/yoken.JJID.2024.108","url":null,"abstract":"Campylobacter jejuni is one of the major bacterial strains that cause diarrhea in humans. It has been associated with many cases of food poisoning in Japan caused by eating raw, undercooked, and/or improperly prepared chicken meat, liver, and grilled chicken (Yakitori). Campylobacter jejuni is also known to be a preceding infectious pathogen of Guillain-Barré syndrome (GBS), which has a considerably negative health impact on humans. In a case of C. jejuni food poisoning that occurred at a restaurant in Tokyo (Japan) in January 2022, 1 of 4 patients with diarrhea developed GBS, which was presumed to have been caused by undercooked chicken, which has emerged as one of the most common causes of food poisoning in Japan. Moreover, C. jejuni isolates from 3 patients, including those with GBS, had the same genotypes (ST22, HS19, and LOS A). This genotype was frequently detected in patients with GBS in the authors' previous study. Findings confirmed that the patient developed GBS due to food poisoning after consuming undercooked chicken.","PeriodicalId":14608,"journal":{"name":"Japanese journal of infectious diseases","volume":" ","pages":"353-355"},"PeriodicalIF":1.3,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141467917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical Efficacy of Therapeutic Agents for Clostridioides difficile Infection Based on Four Severity Classifications. 基于四种严重程度分类的艰难梭菌感染治疗药物的临床疗效。

IF 1.3 4区医学

Japanese journal of infectious diseases Pub Date : 2024-09-19 Epub Date: 2024-03-29 DOI: 10.7883/yoken.JJID.2023.483

Mariko Ohtani, Sadako Yoshizawa, Taito Miyazaki, Eri Kumade, Shinobu Hirayama, Maki Sakamoto, Hinako Murakami, Tadashi Maeda, Yoshikazu Ishii, Takahiro Matsumoto, Kazuhiro Tateda

{"title":"Clinical Efficacy of Therapeutic Agents for Clostridioides difficile Infection Based on Four Severity Classifications.","authors":"Mariko Ohtani, Sadako Yoshizawa, Taito Miyazaki, Eri Kumade, Shinobu Hirayama, Maki Sakamoto, Hinako Murakami, Tadashi Maeda, Yoshikazu Ishii, Takahiro Matsumoto, Kazuhiro Tateda","doi":"10.7883/yoken.JJID.2023.483","DOIUrl":"10.7883/yoken.JJID.2023.483","url":null,"abstract":"Japanese guidelines recommend metronidazole (MNZ) and vancomycin (VCM) for non-severe and severe cases of Clostridioides difficile infection (CDI), respectively. In the present study, we investigated the use of CDI antimicrobials and evaluated their clinical efficacy and validity using four severity classifications. A retrospective chart review was conducted using the data of 137 inpatients with initially positive C. difficile toxin test results and the initiation of CDI antimicrobials between April 2015 and March 2019. Patients treated with VCM or oral MNZ were included for clinical efficacy analysis of CDI antimicrobials and validation of severity classifications. The endpoints were CDI recurrence, 30-day mortality, and diarrhea cure rates. No significant differences were found between the VCM and oral MNZ groups in the CDI recurrence rate (10.4% vs. 12.7%, P = 0.707), 30-day mortality rate (12.5% vs. 5.6%, P = 0.162), and diarrhea cure rate (61.9% vs. 72.7%, P = 0.238), regardless of severity. Treatment with oral MNZ for non-severe cases was promising, confirming its usefulness according to Japanese guidelines. Further investigation of the clinical efficacy of oral MNZ in patients with first-episode CDI and evaluation of the preferred severity classification are warranted.","PeriodicalId":14608,"journal":{"name":"Japanese journal of infectious diseases","volume":" ","pages":"281-284"},"PeriodicalIF":1.3,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140331714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Continuous Renal Replacement Therapy Improves Indicators and Short-Term Survival in People with AIDS Manifesting Sepsis and Acute Kidney Injury. 持续性肾脏替代疗法可改善出现败血症和急性肾损伤的艾滋病患者的指标和短期存活率。

IF 1.3 4区医学

Japanese journal of infectious diseases Pub Date : 2024-07-24 Epub Date: 2024-02-29 DOI: 10.7883/yoken.JJID.2023.280

Jie Zheng, Qiu-Jin Yang, Fei Qi, Han-Zhang Shen, Le Zhang, Jia-Wei Xia

{"title":"Continuous Renal Replacement Therapy Improves Indicators and Short-Term Survival in People with AIDS Manifesting Sepsis and Acute Kidney Injury.","authors":"Jie Zheng, Qiu-Jin Yang, Fei Qi, Han-Zhang Shen, Le Zhang, Jia-Wei Xia","doi":"10.7883/yoken.JJID.2023.280","DOIUrl":"10.7883/yoken.JJID.2023.280","url":null,"abstract":"Patients with acquired immune deficiency syndrome (AIDS) are susceptible to numerous complications, such as sepsis and acute kidney injury (AKI), leading to adverse outcomes. Continuous renal replacement therapy (CRRT) is becoming increasingly popular for treating sepsis and AKI. This study aimed to verify the effectiveness of CRRT in the treatment of patients with AIDS with sepsis and AKI to provide new directions for the treatment of severe AIDS. Data of 74 people with AIDS, sepsis, and AKI were collected. The patients were divided into CRRT and non-CRRT groups. There was no difference in the indicators between the two groups at admission. Vital signs, pH, serum potassium level, renal function, blood lactate level, acute physiology and chronic health evaluation II score, and sequential organ failure assessment score in the CRRT group demonstrated significant improvements over those in the non-CRRT group at both 24 and 72 h after admission (P < 0.05). The levels of interleukin 6 and procalcitonin declined more significantly in the CRRT group at 72 h after admission (P < 0.05). The CRRT group had a higher 28-day survival rate than the non-CRRT group (P < 0.05). CRRT improves the clinical indicators and increases the short-term survival rate of patients with AIDS, sepsis, and AKI.","PeriodicalId":14608,"journal":{"name":"Japanese journal of infectious diseases","volume":" ","pages":"240-243"},"PeriodicalIF":1.3,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139990066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical Course and Molecular Characterization of Human Bocavirus Associated with Acute Lower Respiratory Tract Infections in a Tertiary Care Hospital in Northern India. 印度北部三级医院中与急性下呼吸道感染相关的人类博卡病毒的临床过程和分子特征。

IF 1.3 4区医学

Japanese journal of infectious diseases Pub Date : 2024-07-23 Epub Date: 2024-02-29 DOI: 10.7883/yoken.JJID.2023.251

Subhabrata Sarkar, Mannat Kang, Suresh Kumar Angurana, Shankar Prasad, Ishani Bora, Pankaj Singh, Vikrant Sharma, Meenakshi Rana, Bhartendu Singh, Muralidharan Jayashree, Radha Kanta Ratho

{"title":"Clinical Course and Molecular Characterization of Human Bocavirus Associated with Acute Lower Respiratory Tract Infections in a Tertiary Care Hospital in Northern India.","authors":"Subhabrata Sarkar, Mannat Kang, Suresh Kumar Angurana, Shankar Prasad, Ishani Bora, Pankaj Singh, Vikrant Sharma, Meenakshi Rana, Bhartendu Singh, Muralidharan Jayashree, Radha Kanta Ratho","doi":"10.7883/yoken.JJID.2023.251","DOIUrl":"10.7883/yoken.JJID.2023.251","url":null,"abstract":"Respiratory samples from 139 hospitalized children were screened for the human bocavirus (HBoV) genome. Positive samples were sequenced for the partial VP1/VP2 gene followed by molecular and phylogenetic analyses. HBoV positivity was noted in 7.2% (10/139) of patients. All HBoV-positive children presented with fever, cough, and respiratory distress (90%, 9/10). Three children developed multisystemic viral illness, with one fatality. Eight children required intensive care management and five required mechanical ventilation. The nucleotide percent identity of the partial VP1/VP2 gene in the HBoV study strains ranged from 97.52% to 99.67%. Non-synonymous mutations in the VP1 protein were T591S (n = 8) and Y517S (n = 1) in the HBoV St1 strain and N475S (n = 8) and S591T (n = 2) in the HBoV St2 strain. One strain showed A556P, H556P, I561S, and M562R non-synonymous mutations. All the study strains belonged to the HBoV1 type. Seven HBoV strains belonged to the same lineage, and three belonged to another lineage. For evolutionary dynamics, GTR+I substitution model with uncorrelated relaxed lognormal clock and Bayesian Skyline tree prior showed 9.0 × 10-4 (95% highest probability density interval: 3.1 × 10-6, 2.1 × 10-3) nucleotide substitutions per site per year. Clinical suspicion and virological screening are necessary to identify HBoV infections in children.","PeriodicalId":14608,"journal":{"name":"Japanese journal of infectious diseases","volume":" ","pages":"227-235"},"PeriodicalIF":1.3,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139990065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dissemination of Urinary Escherichia coli Phylogroup B2 in Provincial and Community Hospitals in Uthai Thani, Central Thailand. 泰国中部乌泰他尼府医院和社区医院尿液大肠埃希氏菌 B2 群的传播。

IF 1.3 4区医学

Japanese journal of infectious diseases Pub Date : 2024-07-23 Epub Date: 2024-02-29 DOI: 10.7883/yoken.JJID.2023.376

Chanihcha Anudit, Pornthip Saraisuwan, Chantana Kimterng, Chanakan Puangmanee, Nicharee Bamphensin, Anusak Kerdsin

{"title":"Dissemination of Urinary Escherichia coli Phylogroup B2 in Provincial and Community Hospitals in Uthai Thani, Central Thailand.","authors":"Chanihcha Anudit, Pornthip Saraisuwan, Chantana Kimterng, Chanakan Puangmanee, Nicharee Bamphensin, Anusak Kerdsin","doi":"10.7883/yoken.JJID.2023.376","DOIUrl":"10.7883/yoken.JJID.2023.376","url":null,"abstract":"Escherichia coli is a Gram-negative bacterium that causes a variety of clinical infections in humans, including diarrhea, sepsis, and urinary tract infection. This bacterium is a common multidrug-resistant threat in community and hospital settings worldwide. This study examined the antimicrobial susceptibility and genetic relationship based on Clermont phylotyping and enterobacterial repetitive intergenic consensus (ERIC)-PCR of 84 E. coli urinary isolates from provincial and community hospitals in Thailand. All isolates were susceptible to nitrofurantoin, and almost all isolates were susceptible to carbapenem, fosfomycin, and amikacin. High resistance rates to fluoroquinolone, ampicillin, and trimethoprim/sulfamethoxazole were observed. Clermont phylogroup B2 was predominant (n = 58). Subtyping of the B2 phylogroup revealed diverse subgroups, of which subgroup V (n = 11), VII (n = 9), III (n = 6), and II (n = 6) were most prevalent. ERIC-PCR showed that the strains of the B2 subgroups III and V were spread between provincial and community hospitals and between hospital wards. This evidence suggests the need for comprehensive infection control monitoring, with strong active surveillance at all hospital levels.","PeriodicalId":14608,"journal":{"name":"Japanese journal of infectious diseases","volume":" ","pages":"220-226"},"PeriodicalIF":1.3,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139990067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First Case of Necrotizing Fasciitis and Septicemia Caused by Pigmentibacter ruber. 首例由鲁氏猪杆菌（Pigmentibacter ruber）引起的坏死性筋膜炎和败血症。

IF 1.3 4区医学

Japanese journal of infectious diseases Pub Date : 2024-07-23 Epub Date: 2024-02-29 DOI: 10.7883/yoken.JJID.2023.370

Ayumi Niwa, Masahiro Hayashi, Jun Yonetamari, Motohiro Nakamura, Yuta Yokobori, Sodai Yokoyama, Mizuki Ogawa, Rina Ichioka, Ryosuke Kikuchi, Hiroyuki Okura, Shinji Ogura, Nobuyuki Tetsuka, Kaori Tanaka, Hisashi Baba

引用次数: 0

Biofilm Eradication and Inhibition of Methicillin-resistant Staphylococcus Clinical Isolates by Curcumin-Chitosan Magnetic Nanoparticles 姜黄素-壳聚糖磁性纳米粒子消除生物膜并抑制耐甲氧西林葡萄球菌临床分离株

IF 2.2 4区医学

Japanese journal of infectious diseases Pub Date : 2024-05-31 DOI: 10.7883/yoken.jjid.2024.034

Humberto Antonio Salazar-Sesatty, Edeer Iván Montoya-Hinojosa, Verónica Villarreal-Salazar, Cynthia Aracely Alvizo-Baez, Adrián Camacho-Ortiz, Luis Daniel Terrazas-Armendariz, Itza Eloisa Luna-Cruz, Juan Manuel Alcocer-González, Licet Villarreal-Treviño, Samantha Flores-Treviño

{"title":"Biofilm Eradication and Inhibition of Methicillin-resistant Staphylococcus Clinical Isolates by Curcumin-Chitosan Magnetic Nanoparticles","authors":"Humberto Antonio Salazar-Sesatty, Edeer Iván Montoya-Hinojosa, Verónica Villarreal-Salazar, Cynthia Aracely Alvizo-Baez, Adrián Camacho-Ortiz, Luis Daniel Terrazas-Armendariz, Itza Eloisa Luna-Cruz, Juan Manuel Alcocer-González, Licet Villarreal-Treviño, Samantha Flores-Treviño","doi":"10.7883/yoken.jjid.2024.034","DOIUrl":"https://doi.org/10.7883/yoken.jjid.2024.034","url":null,"abstract":"Biofilm-producing methicillin-resistant Staphylococcus aureus (MRSA) and coagulase-negative Staphylococci (MR-CoNS) are a clinical challenge for the treatment of healthcare-associated infections. As alternative antimicrobial options are needed, we aimed to determine the effect of curcumin-chitosan magnetic nanoparticles on the biofilm of staphylococcal clinical isolates. MRSA and CoNS clinical isolates were identified by MALDI-TOF mass spectrometry. Antimicrobial susceptibility testing was performed by broth microdilution. Nanoparticles were synthesized by co-precipitation of magnetic nanoparticles (MNP) and encapsulation by ionotropic gelation of curcumin (Cur) and chitosan (Chi). Biofilm inhibition and eradication by nanoparticles with and without the addition of oxacillin was assessed on staphylococcal strains. Cur-Chi-MNP showed antimicrobial activity on planktonic cells of MRSA and MR-CoNS strains and inhibited biofilm of MRSA. The addition of OXA to Cur-Chi-MNP increased biofilm inhibition and eradication activity against all Staphylococci strains (p=0.0007); higher biofilm activity was observed in early biofilm stages. Cur-Chi-MNP showed antimicrobial and biofilm inhibition activity against S. aureus. The addition of OXA increased biofilm inhibition and eradication activity against all Staphylococci strains. A combination treatment of Cur-Chi-MNP and OXA could be potentially used to treat staphylococcal biofilm-associated infections in its early stages before the establishment of biofilm bacterial cells.\u0000","PeriodicalId":14608,"journal":{"name":"Japanese journal of infectious diseases","volume":"64 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141198126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0