Detection of Human Coronavirus-OC43 in Nasopharyngeal Swab Specimens Via Immunofluorescence Staining Using Human Serum and an Anti-human Antibody.

IF 1.3 4区 医学 Q4 INFECTIOUS DISEASES
Japanese journal of infectious diseases Pub Date : 2025-03-21 Epub Date: 2024-11-29 DOI:10.7883/yoken.JJID.2024.001
Mutsuo Yamaya, Oshi Watanabe, Yuki Kitai, Yusuke Sayama, Suguru Ohmiya, Hidekazu Nishimura
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Abstract

Immunofluorescence methods using cell lines to detect seasonal human coronavirus (HCoV)-OC43 in nasopharyngeal swab specimens have not yet been established. A human rectal adenocarcinoma cell line (HRT-18) was exposed to the specimens obtained from patients with upper respiratory tract infections. Immunofluorescence staining was conducted using a combination of human serum containing an HCoV-OC43 anti-spike protein antibody and a fluorescence-labeled anti-human antibody. Positive staining in HRT-18 cells was detected after exposure to specimens obtained from nine of eleven patients in whom HCoV-OC43 RNA was detected using the FilmArray method. Increased viral RNA levels in the supernatant were also detected in HRT-18 cells exposed to specimens obtained from four of five patients. In contrast, positive staining was not detected in HRT-18 cells exposed to six patient specimens that tested negative for RNA from 17 types and subtypes of respiratory viruses, including HCoV-OC43. Cells inoculated with the established strain HCoV-OC43 (ATCC VR-759) also showed positive staining. These findings suggest that replication-competent HCoV-OC43 in the specimens can be detected via immunofluorescence staining of HRT-18 cells with human serum. Using this method, positive staining for viruses other than HCoV-OC43 may be obtained.

人血清和抗人抗体免疫荧光染色法检测鼻咽拭子标本中人冠状病毒oc43
利用细胞系检测鼻咽拭子标本中季节性人冠状病毒(HCoV)-OC43的免疫荧光方法尚未建立。将一株人直肠腺癌细胞(HRT-18)暴露于上呼吸道感染患者的标本中。用含HCoV-OC43抗刺突蛋白抗体的人血清与荧光标记的抗人抗体联合进行免疫荧光染色。在使用FilmArray方法检测HCoV-OC43 RNA的11例患者中,有9例患者的标本暴露后,检测到HRT-18细胞呈阳性染色。在暴露于从5名患者中4名获得的标本的HRT-18细胞中也检测到上清病毒RNA水平升高。相比之下,暴露于包括HCoV-OC43在内的17种呼吸道病毒类型和亚型的患者标本中的HRT-18细胞未检测到阳性染色。用已建立的菌株HCoV-OC43 (ATCC VR-759)接种的细胞也呈阳性染色。结果表明,HRT-18细胞经人血清免疫荧光染色,可检测到HCoV-OC43的复制能力。使用这种方法可以获得HCoV-OC43以外病毒的阳性染色。
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来源期刊
CiteScore
4.50
自引率
4.50%
发文量
172
审稿时长
2 months
期刊介绍: Japanese Journal of Infectious Diseases (JJID), an official bimonthly publication of National Institute of Infectious Diseases, Japan, publishes papers dealing with basic research on infectious diseases relevant to humans in the fields of bacteriology, virology, mycology, parasitology, medical entomology, vaccinology, and toxinology. Pathology, immunology, biochemistry, and blood safety related to microbial pathogens are among the fields covered. Sections include: original papers, short communications, epidemiological reports, methods, laboratory and epidemiology communications, letters to the editor, and reviews.
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