{"title":"Detection of Human Coronavirus-OC43 in Nasopharyngeal Swab Specimens Via Immunofluorescence Staining Using Human Serum and an Anti-human Antibody.","authors":"Mutsuo Yamaya, Oshi Watanabe, Yuki Kitai, Yusuke Sayama, Suguru Ohmiya, Hidekazu Nishimura","doi":"10.7883/yoken.JJID.2024.001","DOIUrl":null,"url":null,"abstract":"<p><p>Immunofluorescence methods using cell lines to detect seasonal human coronavirus (HCoV)-OC43 in nasopharyngeal swab specimens have not yet been established. A human rectal adenocarcinoma cell line (HRT-18) was exposed to the specimens obtained from patients with upper respiratory tract infections. Immunofluorescence staining was conducted using a combination of human serum containing an HCoV-OC43 anti-spike protein antibody and a fluorescence-labeled anti-human antibody. Positive staining in HRT-18 cells was detected after exposure to specimens obtained from nine of eleven patients in whom HCoV-OC43 RNA was detected using the FilmArray method. Increased viral RNA levels in the supernatant were also detected in HRT-18 cells exposed to specimens obtained from four of five patients. In contrast, positive staining was not detected in HRT-18 cells exposed to six patient specimens that tested negative for RNA from 17 types and subtypes of respiratory viruses, including HCoV-OC43. Cells inoculated with the established strain HCoV-OC43 (ATCC VR-759) also showed positive staining. These findings suggest that replication-competent HCoV-OC43 in the specimens can be detected via immunofluorescence staining of HRT-18 cells with human serum. Using this method, positive staining for viruses other than HCoV-OC43 may be obtained.</p>","PeriodicalId":14608,"journal":{"name":"Japanese journal of infectious diseases","volume":" ","pages":"91-98"},"PeriodicalIF":1.3000,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Japanese journal of infectious diseases","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.7883/yoken.JJID.2024.001","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/11/29 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
引用次数: 0
Abstract
Immunofluorescence methods using cell lines to detect seasonal human coronavirus (HCoV)-OC43 in nasopharyngeal swab specimens have not yet been established. A human rectal adenocarcinoma cell line (HRT-18) was exposed to the specimens obtained from patients with upper respiratory tract infections. Immunofluorescence staining was conducted using a combination of human serum containing an HCoV-OC43 anti-spike protein antibody and a fluorescence-labeled anti-human antibody. Positive staining in HRT-18 cells was detected after exposure to specimens obtained from nine of eleven patients in whom HCoV-OC43 RNA was detected using the FilmArray method. Increased viral RNA levels in the supernatant were also detected in HRT-18 cells exposed to specimens obtained from four of five patients. In contrast, positive staining was not detected in HRT-18 cells exposed to six patient specimens that tested negative for RNA from 17 types and subtypes of respiratory viruses, including HCoV-OC43. Cells inoculated with the established strain HCoV-OC43 (ATCC VR-759) also showed positive staining. These findings suggest that replication-competent HCoV-OC43 in the specimens can be detected via immunofluorescence staining of HRT-18 cells with human serum. Using this method, positive staining for viruses other than HCoV-OC43 may be obtained.
期刊介绍:
Japanese Journal of Infectious Diseases (JJID), an official bimonthly publication of National Institute of Infectious Diseases, Japan, publishes papers dealing with basic research on infectious diseases relevant to humans in the fields of bacteriology, virology, mycology, parasitology, medical entomology, vaccinology, and toxinology. Pathology, immunology, biochemistry, and blood safety related to microbial pathogens are among the fields covered. Sections include: original papers, short communications, epidemiological reports, methods, laboratory and epidemiology communications, letters to the editor, and reviews.