Intervirology最新文献_第8页

Phylogenetic Characterization of the 5' Untranslated Region of Untypable HCV Genotypes Circulating in Pakistan. 巴基斯坦流行的无法识别的 HCV 基因型的 5' 非翻译区系统发育特征。

IF 4.6 4区医学

Intervirology Pub Date : 2020-12-22 DOI: 10.1159/000512133

Usman Ghani, Irshad Ur Rehman, Muhammad Ali, Ayyaz Khan, Sana Ullah, Liaqat Ali, Sadia Butt

{"title":"Phylogenetic Characterization of the 5' Untranslated Region of Untypable HCV Genotypes Circulating in Pakistan.","authors":"Usman Ghani, Irshad Ur Rehman, Muhammad Ali, Ayyaz Khan, Sana Ullah, Liaqat Ali, Sadia Butt","doi":"10.1159/000512133","DOIUrl":"10.1159/000512133","url":null,"abstract":"Introduction: Commercial methods for HCV genotyping is challenged by the increased prevalence of untypable genotypes in Pakistan.Objective: The aim of the current study was to perform nucleotide sequencing of 5' UTR region for genotyping of viral isolates circulating in Peshawar, Pakistan.Methods: The total number of commercially untypable samples were 94 in which 18 samples were sequenced for the characterization of 5' UTR region. Post-sequencing analysis was performed for genotype identification (n = 18) and molecular phylogenetic analysis.Results: The current study reveals different genotypes, that is, 10/18 viral isolates were found to be genotype 3a (55.55%), 3 isolates (genotype 3b, 16.66%), 2 isolates (genotype 6h/6g, 11.11%), 2 (6g/d, 11.11%), and 1 sample (genotype 1c, 5.55%). In addition, genotype 3a is the dominant representative of HCV circulating in Pakistan and has been increasing across the country.Conclusion: The current study also reveals that genotype 6 (2 were genotype 6h/6g and 2 were 6g/d) is also circulating in Pakistan and not restricted to South China and Hong Kong.","PeriodicalId":14547,"journal":{"name":"Intervirology","volume":" ","pages":"1-6"},"PeriodicalIF":4.6,"publicationDate":"2020-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39090745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Survey of BK Virus in Renal Transplant Recipients in Iran: A Systematic Review and Meta-Analysis. 伊朗肾移植受者 BK 病毒调查：系统回顾与元分析》。

IF 4.6 4区医学

Intervirology Pub Date : 2020-12-17 DOI: 10.1159/000512132

Somayeh Shatizadeh Malekshahi, Hoorieh Soleimanjahi, Fariba Dorostkar, Vahid Salimi, Mohammad Farahmand

{"title":"Survey of BK Virus in Renal Transplant Recipients in Iran: A Systematic Review and Meta-Analysis.","authors":"Somayeh Shatizadeh Malekshahi, Hoorieh Soleimanjahi, Fariba Dorostkar, Vahid Salimi, Mohammad Farahmand","doi":"10.1159/000512132","DOIUrl":"10.1159/000512132","url":null,"abstract":"Introduction: BK virus (BKV) infection in renal transplant (RT) recipients can cause hemorrhagic cystitis, transient renal dysfunction, and BKV nephropathy (BKVN). The prevalence and significance of BKV in RT recipients remain to be clarified in the Iranian population. The purpose of this review is to summarize the overall prevalence of BKV infection in RT recipients from previously published studies in Iran.Methods: We systematically reviewed articles through a comprehensive search of the main electronic and Persian national databases up to November 2019.Results: The overall pooled prevalence of BKV infection among the Iranian population was 23% (95% CI; 15-33%). Comparing these studies revealed that the prevalence of BKV in plasma samples ranges from 3 to 40%, in renal biopsies 1-13%, and in urine samples 10-49%. Due to substantial heterogeneity among reported studies (I2 = 93%, p < 0.01), random-effect meta-analysis was performed. BKV infection rate was slightly higher in women than men (16%, p = 0.04 vs. 14%, p < 0.01, respectively). The majority of the studies employed real-time PCR (24%, I2 = 93, p < 0.01) and analyzed plasma samples alone or in combination with other types of specimens. BKV prevalence from 5 cities among the Iranian population showed a higher prevalence rate in Guilan.Conclusion: Our analysis provides a preliminary estimate of the epidemiology of BKV infection in RT recipients in Iran. These results arouse a need for more epidemiological studies of BKV infection in different unanalyzed regions in Iran. Early detection of BKV in RT recipients helps timely nephropathy diagnosis and prevents graft loss.","PeriodicalId":14547,"journal":{"name":"Intervirology","volume":" ","pages":"1-9"},"PeriodicalIF":4.6,"publicationDate":"2020-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38735181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chandipura Virus' Oncolytic Potential in Experimentally Induced Tumor in Mice. 钱迪普拉病毒在小鼠实验性肿瘤中的溶瘤潜力

IF 4.6 4区医学

Intervirology Pub Date : 2020-12-15 DOI: 10.1159/000512299

Anukumar Balakrishnan, Nikita Malik

引用次数: 0

Havachoobe (Onosma dichroanthum Boiss) Root Extract Decreases the Hepatitis B Virus Surface Antigen Secretion in the PLC/PRF/5 Cell Line. Havachoobe（Onosma dichroanthum Boiss）根提取物可减少 PLC/PRF/5 细胞系中乙型肝炎病毒表面抗原的分泌。

IF 4.6 4区医学

Intervirology Pub Date : 2020-12-15 DOI: 10.1159/000512140

Alireza Mohebbi, Fahimeh Azadi, Mohammad Mostakhdem Hashemi, Fatemeh Sana Askari, Nazanin Razzaghi

{"title":"Havachoobe (Onosma dichroanthum Boiss) Root Extract Decreases the Hepatitis B Virus Surface Antigen Secretion in the PLC/PRF/5 Cell Line.","authors":"Alireza Mohebbi, Fahimeh Azadi, Mohammad Mostakhdem Hashemi, Fatemeh Sana Askari, Nazanin Razzaghi","doi":"10.1159/000512140","DOIUrl":"10.1159/000512140","url":null,"abstract":"Background: Many efforts are currently focused on functional treatment of the hepatitis B virus (HBV). This can be done by suppressing the secretion of HBV surface antigen (HBsAg). Scientific communities are very interested in natural products in that respect.Objective: Use of root extract of Havachoobe (Onosma dichroanthum BoissI), a Northern Iranian native medical herb, for assessment of its anti-HBsAg secretion activity.Methods: Havachoobe had been bought at a nearby apothecary store. Plant root extract was obtained using a hydroalcoholic process. Cytotoxic activity of the extract was examined on PLC/PRF/5 cells using MTT assay. ELISA has been used to measure HBsAg in the treated cell line supernatants. In addition, real-time PCR analysis was performed to evaluate the expression of HBsAg before and after treatment of Onosma in vitro.Results: The results showed very low root extract cytotoxicity at concentrations under 8 μg/mL. Tissue culture infectious dose 50 was obtained at 63.78 μg/mL. In a dose-dependent and time-dependent manner, a significantly reduced HBsAg secretion was observed at a concentration of 8 ppm at 12 h post-treatment. The real-time PCR result showed relative decreased HBsAg expression at all doses at 12 h post-treatment time.Discussion: In this study, we first reported anti-HBsAg activity on an Iranian herbal medicine. Havachoobe root extract was shown to be able to inhibit HBsAg in a dose-dependent and time-dependent manner. We find the extract exerts its inhibitory effect of HBsAg by targeting transcription of HBsAg.","PeriodicalId":14547,"journal":{"name":"Intervirology","volume":" ","pages":"1-5"},"PeriodicalIF":4.6,"publicationDate":"2020-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38716131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Available Antiretrovirals in the Treatment and Pre-Exposure Prophylaxis of SARS-CoV-2: Quo Vadis? 现有抗逆转录病毒药物在 SARS-CoV-2 治疗和暴露前预防中的应用：何去何从？

IF 4.6 4区医学

Intervirology Pub Date : 2020-12-11 DOI: 10.1159/000512134

Miguel Alpalhão, Paulo Filipe

引用次数: 0

Association of Sex, Age, and Comorbidities with Mortality in COVID-19 Patients: A Systematic Review and Meta-Analysis. 性别、年龄和合并症与COVID-19患者死亡率的关系:一项系统综述和荟萃分析

IF 4.6 4区医学

Intervirology Pub Date : 2020-12-09 DOI: 10.1159/000512592

Mohitosh Biswas, Shawonur Rahaman, Tapash Kumar Biswas, Zahirul Haque, Baharudin Ibrahim

{"title":"Association of Sex, Age, and Comorbidities with Mortality in COVID-19 Patients: A Systematic Review and Meta-Analysis.","authors":"Mohitosh Biswas, Shawonur Rahaman, Tapash Kumar Biswas, Zahirul Haque, Baharudin Ibrahim","doi":"10.1159/000512592","DOIUrl":"10.1159/000512592","url":null,"abstract":"Introduction: Although severe acute respiratory syndrome coronavirus-2 infection is causing mortality in considerable proportion of coronavirus disease-2019 (COVID-19) patients, however, evidence for the association of sex, age, and comorbidities on the risk of mortality is not well-aggregated yet. It was aimed to assess the association of sex, age, and comorbidities with mortality in COVID-2019 patients.Methods: Literatures were searched using different keywords in various databases. Relative risks (RRs) were calculated by RevMan software where statistical significance was set as p < 0.05.Results: COVID-19 male patients were associated with significantly increased risk of mortality compared to females (RR 1.86: 95% confidence interval [CI] 1.67-2.07; p < 0.00001). Patients with age ≥50 years were associated with 15.4-folds significantly increased risk of mortality compared to patients with age <50 years (RR 15.44: 95% CI 13.02-18.31; p < 0.00001). Comorbidities were also associated with significantly increased risk of mortality; kidney disease (RR 4.90: 95% CI 3.04-7.88; p < 0.00001), cereborovascular disease (RR 4.78; 95% CI 3.39-6.76; p < 0.00001), cardiovascular disease (RR 3.05: 95% CI 2.20-4.25; p < 0.00001), respiratory disease (RR 2.74: 95% CI 2.04-3.67; p < 0.00001), diabetes (RR 1.97: 95% CI 1.48-2.64; p < 0.00001), hypertension (RR 1.95: 95% CI 1.58-2.40; p < 0.00001), and cancer (RR 1.89; 95% CI 1.25-2.84; p = 0.002) but not liver disease (RR 1.64: 95% CI 0.82-3.28; p= 0.16).Conclusion: Implementation of adequate protection and interventions for COVID-19 patients in general and in particular male patients with age ≥50 years having comorbidities may significantly reduce risk of mortality associated with COVID-19.","PeriodicalId":14547,"journal":{"name":"Intervirology","volume":" ","pages":"1-12"},"PeriodicalIF":4.6,"publicationDate":"2020-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7801974/pdf/int-0001.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38703652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Front & Back Matter 正面和背面

IF 4.6 4区医学

Intervirology Pub Date : 2020-12-01 DOI: 10.1159/000513757

J. Manuguerra, U. Liebert

引用次数: 0

Contents Vol. 63, 2020 目录2020年第63卷

IF 4.6 4区医学

Intervirology Pub Date : 2020-12-01 DOI: 10.1159/000513437

引用次数: 0

Front & Back Matter 正面和背面事项

IF 4.6 4区医学