Phylogenetic Characterization of the 5' Untranslated Region of Untypable HCV Genotypes Circulating in Pakistan.

IF 4.3 3区材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC

ACS Applied Electronic Materials Pub Date : 2020-12-22 DOI:10.1159/000512133

Usman Ghani, Irshad Ur Rehman, Muhammad Ali, Ayyaz Khan, Sana Ullah, Liaqat Ali, Sadia Butt

{"title":"Phylogenetic Characterization of the 5' Untranslated Region of Untypable HCV Genotypes Circulating in Pakistan.","authors":"Usman Ghani, Irshad Ur Rehman, Muhammad Ali, Ayyaz Khan, Sana Ullah, Liaqat Ali, Sadia Butt","doi":"10.1159/000512133","DOIUrl":null,"url":null,"abstract":"Introduction: Commercial methods for HCV genotyping is challenged by the increased prevalence of untypable genotypes in Pakistan.Objective: The aim of the current study was to perform nucleotide sequencing of 5' UTR region for genotyping of viral isolates circulating in Peshawar, Pakistan.Methods: The total number of commercially untypable samples were 94 in which 18 samples were sequenced for the characterization of 5' UTR region. Post-sequencing analysis was performed for genotype identification (n = 18) and molecular phylogenetic analysis.Results: The current study reveals different genotypes, that is, 10/18 viral isolates were found to be genotype 3a (55.55%), 3 isolates (genotype 3b, 16.66%), 2 isolates (genotype 6h/6g, 11.11%), 2 (6g/d, 11.11%), and 1 sample (genotype 1c, 5.55%). In addition, genotype 3a is the dominant representative of HCV circulating in Pakistan and has been increasing across the country.Conclusion: The current study also reveals that genotype 6 (2 were genotype 6h/6g and 2 were 6g/d) is also circulating in Pakistan and not restricted to South China and Hong Kong.","PeriodicalId":3,"journal":{"name":"ACS Applied Electronic Materials","volume":null,"pages":null},"PeriodicalIF":4.3000,"publicationDate":"2020-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Electronic Materials","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1159/000512133","RegionNum":3,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, ELECTRICAL & ELECTRONIC","Score":null,"Total":0}

引用次数: 0

Abstract

Introduction: Commercial methods for HCV genotyping is challenged by the increased prevalence of untypable genotypes in Pakistan.

Objective: The aim of the current study was to perform nucleotide sequencing of 5' UTR region for genotyping of viral isolates circulating in Peshawar, Pakistan.

Methods: The total number of commercially untypable samples were 94 in which 18 samples were sequenced for the characterization of 5' UTR region. Post-sequencing analysis was performed for genotype identification (n = 18) and molecular phylogenetic analysis.

Results: The current study reveals different genotypes, that is, 10/18 viral isolates were found to be genotype 3a (55.55%), 3 isolates (genotype 3b, 16.66%), 2 isolates (genotype 6h/6g, 11.11%), 2 (6g/d, 11.11%), and 1 sample (genotype 1c, 5.55%). In addition, genotype 3a is the dominant representative of HCV circulating in Pakistan and has been increasing across the country.

Conclusion: The current study also reveals that genotype 6 (2 were genotype 6h/6g and 2 were 6g/d) is also circulating in Pakistan and not restricted to South China and Hong Kong.

查看原文本刊更多论文

巴基斯坦流行的无法识别的 HCV 基因型的 5' 非翻译区系统发育特征。

导言：在巴基斯坦，由于不可分型基因型的流行率增加，HCV 基因分型的商业方法面临挑战：本研究旨在对巴基斯坦白沙瓦市流行的病毒分离株进行 5' UTR 区域核苷酸测序，以进行基因分型：方法：共采集了 94 份商业上无法分型的样本，并对其中 18 份样本的 5' UTR 区域进行了测序。测序后进行了基因型鉴定（n = 18）和分子系统学分析：目前的研究揭示了不同的基因型，即发现 10/18 个病毒分离株为基因型 3a（55.55%）、3 个分离株（基因型 3b，16.66%）、2 个分离株（基因型 6h/6g，11.11%）、2 个（6g/d，11.11%）和 1 个样本（基因型 1c，5.55%）。此外，基因型 3a 是在巴基斯坦流行的 HCV 的主要代表，并且在全国范围内呈上升趋势：目前的研究还显示，基因型 6（2 例为基因型 6h/6g，2 例为基因型 6g/d）也在巴基斯坦流行，并不局限于华南地区和香港。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

ACS Applied Electronic Materials Multiple-

CiteScore

7.20

自引率

4.30%

发文量

567