Iranian Journal of Basic Medical Sciences最新文献_第5页

Rutin attenuates complete Freund's adjuvant-induced inflammatory pain in rats.

IF 2.1 4区医学

Iranian Journal of Basic Medical Sciences Pub Date : 2025-01-01 DOI: 10.22038/ijbms.2024.81572.17655

Fatemeh Forouzanfar, Ali Mohammad Pourbagher-Shahri, Amir Mahmoud Ahmadzadeh

{"title":"Rutin attenuates complete Freund's adjuvant-induced inflammatory pain in rats.","authors":"Fatemeh Forouzanfar, Ali Mohammad Pourbagher-Shahri, Amir Mahmoud Ahmadzadeh","doi":"10.22038/ijbms.2024.81572.17655","DOIUrl":"10.22038/ijbms.2024.81572.17655","url":null,"abstract":"Objectives: Rutin is a bioflavonoid compound renowned for its anti-oxidative, anti-inflammatory, and antinociceptive properties. The present study aims to assess its therapeutic efficacy on complete Freund's adjuvant (CFA)induced inflammatory pain.Materials and methods: Arthritis was induced in Wistar rats via subcutaneous administration of CFA into the right hind paw. Rutin (15 and 30 mg/kg) and indomethacin (5 mg/kg, orally) were given once daily for three weeks. Parameters observed included alterations in paw swelling perimeter, arthritis scores, and body weight. Additionally, antinociceptive activity was measured through thermal hyperalgesia and cold allodynia responses. The Tumor necrosis factor-alpha (TNF-α) level in the serum was measured. Malondialdehyde (MDA), thiol levels, catalase, and superoxide dismutase (SOD) activities were also evaluated as serum oxidative stress markers.Results: Rutin and indomethacin significantly suppressed alterations in paw edema, pain responses, and arthritis scores and reduced the loss of body weight in contrast to disease-control rats. Furthermore, in contrast to disease control rats, rutin and indomethacin treatment exhibited an anti-inflammatory effect through a marked reduction in TNF-α levels in the serum. Rutin and indomethacin demonstrated a significant increase in catalase and SOD activities, a total thiol level, and a decrease in MDA level compared to the disease-control rats.Conclusion: These results suggest that rutin's antiarthritic effect is mediated by its antinociceptive, anti-oxidant, and anti-inflammatory properties.","PeriodicalId":14495,"journal":{"name":"Iranian Journal of Basic Medical Sciences","volume":"28 3","pages":"332-339"},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11790188/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143189087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rosmarinic acid attenuated inflammation and apoptosis in folic acid-induced renal injury: Role of FoxO3/ NFκB pathway.

IF 2.1 4区医学

Iranian Journal of Basic Medical Sciences Pub Date : 2025-01-01 DOI: 10.22038/ijbms.2024.80551.17436

Maryam Mottaghi, Fatemeh Heidari, Tahereh Komeili Movahed, Akram Eidi, Azam Moslehi

{"title":"Rosmarinic acid attenuated inflammation and apoptosis in folic acid-induced renal injury: Role of FoxO3/ NFκB pathway.","authors":"Maryam Mottaghi, Fatemeh Heidari, Tahereh Komeili Movahed, Akram Eidi, Azam Moslehi","doi":"10.22038/ijbms.2024.80551.17436","DOIUrl":"10.22038/ijbms.2024.80551.17436","url":null,"abstract":"Objectives: Rosmarinic acid (RA) is a herbal compound with various antioxidant and anti-inflammatory effects. This study aimed to explore the anti-inflammatory and anti-apoptotic properties of RA in folic acid-induced renal injury.Materials and methods: Thirty-six male C57/BL6 mice were randomly divided into six groups (N=6): Control (received normal saline), NaHCO3 (received NaHCO3 as folic acid solvent), FA (received folic acid (FA)(IP) to induce renal injury), RA (received 100 mg/kg RA), RA50-FA (received 50 mg/kg RA solution after folic acid injection), and RA100-FA (received 100 mg/kg RA after folic acid injection). For ten days, the treatment groups received RA by gavage. The effects of RA were assessed using H & E staining, biochemical tests, western blotting, and ELISA in the kidney tissues of the mice. Real-time RT-PCR was also performed to evaluate the expression changes of renal genes.Results: Our data showed that treatment by RA led to the over-expression of FoxO3 (P<0.05) and decrease in NFκB levels (P<0.01 and P<0.05) and expression of TNFα (P<0.05) and IL6 (P<0.001 and P<0.01). Other evaluations showed a decrease in p53 (P<0.01 and P<0.001), Bax/Bcl-2 ratio expression (P<0.01 and P<0.05), and Caspase-3 level (P<0.01 and P<0.05) compared to the folic acid group. Histological and biochemical results also confirmed the attenuation of tissue damage.Conclusion: This study revealed that RA's positive effects on folic acid-induced renal injury might result from the involvement of the FoxO3/NFκB pathway, thereby suppressing inflammation and apoptosis.","PeriodicalId":14495,"journal":{"name":"Iranian Journal of Basic Medical Sciences","volume":"28 3","pages":"316-322"},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11790193/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143189140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective immune response induced by cationic liposomes bearing soluble antigens improves the survival of BALB/c mice against Toxoplasma gondii RH strain.

IF 2.1 4区医学

Iranian Journal of Basic Medical Sciences Pub Date : 2025-01-01 DOI: 10.22038/ijbms.2024.82123.17770

Hadi Mirahmadi, Ahmad Mehravaran, Mahdi Kavand, Ali Reza Salimi Khorashad, Nasrin Rezaee

{"title":"Protective immune response induced by cationic liposomes bearing soluble antigens improves the survival of BALB/c mice against Toxoplasma gondii RH strain.","authors":"Hadi Mirahmadi, Ahmad Mehravaran, Mahdi Kavand, Ali Reza Salimi Khorashad, Nasrin Rezaee","doi":"10.22038/ijbms.2024.82123.17770","DOIUrl":"10.22038/ijbms.2024.82123.17770","url":null,"abstract":"Objectives: An ideal strategy to control acute or chronic toxoplasmosis can be the development and production of an effective vaccine. Liposomes as immunoadjuvants may be utilized to boost immune reactions for various antigens.Materials and methods: In this study, we encapsulated soluble Toxoplasma antigen (SA) in 1, 2-Dioleoyl-3-trimethylammonium propane (DOTAP) liposomes to assess the elicited immunological response. BALB/C mice received three intramuscular injections of various formulations separated by two weeks. The kind of immune reaction that was created, the degree of protection, the percentage of BALB/c mice that survived the Toxoplasma gondii challenge, the immune reaction assessment with cytokine synthesis (IFN-γ, IL-4), and the titration of IgG isotypes were all evaluated.Results: Compared to other groups, the liposome DOTAP + imiquimod + SA-immunized mice showed a significantly lower death rate (P<0.01). Liposome DOTAP + Imiquimod + SA had higher IgG2a and IFN-γ secretion levels than the control group (P<0.001 and P<0.0001, respectively).Conclusion: According to the study's findings, the liposome DOTAP + imiquimod + SA formulation generates a cellular immunological response, making it resistant to the T. gondii challenge.","PeriodicalId":14495,"journal":{"name":"Iranian Journal of Basic Medical Sciences","volume":"28 3","pages":"347-354"},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11790195/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143189068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characteristics of soybean (Glycine max) protein hydrolysate by bromelain and its effect on inflammation and kidney disorders in gentamicin-induced male Wistar rats.

IF 2.1 4区医学

Iranian Journal of Basic Medical Sciences Pub Date : 2025-01-01 DOI: 10.22038/ijbms.2024.78131.16885

Meilinah Hidayat, Amirah Barnas, Virginia Nussy, Timothy Wantania, Sijani Prahastuti, Sriwidodo Sriwidodo, Khomaini Hasan

{"title":"Characteristics of soybean (Glycine max) protein hydrolysate by bromelain and its effect on inflammation and kidney disorders in gentamicin-induced male Wistar rats.","authors":"Meilinah Hidayat, Amirah Barnas, Virginia Nussy, Timothy Wantania, Sijani Prahastuti, Sriwidodo Sriwidodo, Khomaini Hasan","doi":"10.22038/ijbms.2024.78131.16885","DOIUrl":"10.22038/ijbms.2024.78131.16885","url":null,"abstract":"Objectives: Soybeans have various positive effects on health, including anti-inflammatory and preventing kidney damage. There is concern regarding the phytoestrogen content due to the high isoflavone content in soybeans. Various forms of soybean processing have been tried; in this study, the hydrolysis method will be used to obtain the active substance Arginine-Glycine-Aspartate (RGD) tripeptide in soybean protein hydrolyzed by bromelain (SPHB). The research aimed to determine the characteristics and influence of SPHB on kidney function, inflammation, body weight, and estrogen in male Wistar rats induced by gentamicin.Materials and methods: Soybeans (Glycine max) are hydrolyzed using the proteolytic enzyme Bromelain from pineapples. The proteomics was investigated using Liquid-Chromatography Mass Spectroscopy Tandem (LC-MS/MS). SPHB in three doses would be tested for 28 days on male Wistar rats induced by gentamicin. The parameters measured were body weight, high-sensitive cell reactive protein (hs-CRP) levels, urea, creatinine, and estrogen levels.Results: SPHB has a low molecular weight (LMW) of 10 kDa and contains RGD in the lunasin sequence. SPHB showed no effect on body weight (P>0.05). The impact of SPHB on hs-CRP, urea, and creatinine showed differences significantly from the positive control, especially SPHB at a dose of 112 mg/day (P<0.01). Meanwhile, SPHB has almost no effect on estrogen levels.Conclusion: The administration of SPHB with LMW and contains lunasin showed decreased inflammation and kidney function parameters but did not affect body weight and estrogen levels in induced gentamicin Wistar rats.","PeriodicalId":14495,"journal":{"name":"Iranian Journal of Basic Medical Sciences","volume":"28 1","pages":"56-62"},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11771338/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143058965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of anemoside B4 on ameliorating cerebral ischemic/reperfusion injury.

IF 2.1 4区医学

Iranian Journal of Basic Medical Sciences Pub Date : 2025-01-01 DOI: 10.22038/ijbms.2024.78569.16991

Huizhi Fei, Xiaohuan Huang

{"title":"Effect of anemoside B4 on ameliorating cerebral ischemic/reperfusion injury.","authors":"Huizhi Fei, Xiaohuan Huang","doi":"10.22038/ijbms.2024.78569.16991","DOIUrl":"10.22038/ijbms.2024.78569.16991","url":null,"abstract":"Objectives: Anemoside B4 (AB4) is a multifunctional compound with anti-inflammatory, anti-apoptotic, antioxidant, antiviral, and autophagy-enhancing effects. However, the role of AB4 in cerebral ischemia/reperfusion injury (CIRI) remains obscure. This experiment aims to investigate the pharmacological effects of AB4 in CIRI.Materials and methods: In vivo, eighty male SD rats were randomly divided into five groups: sham, MCAO/R, LD group (2.5 mg/kg), MD group (5 mg/kg), and HD group (10 mg/kg). The rats in sham and MCAO/R groups were given equal volumes of normal saline. In vitro, PC12 cells were divided into five groups: normal, OGD/R, OGD/R+AB4 (50 μM), OGD/R+AB4 (100 μM), and OGD/R+AB4 (200 μM). The cells were treated with hypoxia and hypoglycemia for 1.5 hr and reoxygenation for 24 hr.Results: In vivo, TTC and neurological scoring tests indicated that AB4 favors promoting the recovery of the brain. The histopathologic study of the brain tissues revealed that AB4 inhibited the damage of neuron cells. The TUNEL assay found that AB4 could improve cell apoptosis and prevent the brain from injury. In vitro, the data showed that AB4 inhibited cell damage and prevented PC12 cells from OGD/R injury, reduced IL-1β content, and increased the IL-10 level. AB4 could inhibit apoptosis of PC12 cells, down-regulate Caspase 12 and BAX expression, and up-regulate Bcl-2 expression.Conclusion: AB4 played a protective role in CIRI and could be a promising active ingredient against ischemia stroke.","PeriodicalId":14495,"journal":{"name":"Iranian Journal of Basic Medical Sciences","volume":"28 1","pages":"49-55"},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11771334/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143058979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Puerarin alleviates renal ischemia/reperfusion injury by inhibiting apoptosis and endoplasmic reticulum stress via Nrf2/HO-1 pathway.

IF 2.1 4区医学

Iranian Journal of Basic Medical Sciences Pub Date : 2025-01-01 DOI: 10.22038/ijbms.2024.80438.17412

Jingsong Wang, Qingyuan Zheng, Zhiyuan Chen, Xiuheng Liu, Shanshan Wan, Lei Wang

{"title":"Puerarin alleviates renal ischemia/reperfusion injury by inhibiting apoptosis and endoplasmic reticulum stress via Nrf2/HO-1 pathway.","authors":"Jingsong Wang, Qingyuan Zheng, Zhiyuan Chen, Xiuheng Liu, Shanshan Wan, Lei Wang","doi":"10.22038/ijbms.2024.80438.17412","DOIUrl":"10.22038/ijbms.2024.80438.17412","url":null,"abstract":"Objectives: To explore the effects of puerarin on renal ischemia/reperfusion injury and the possible mechanism.Materials and methods: The experimental mice were injected with puerarin (50 or 100 mg/kg) per day or equal sterile saline by intraperitoneal injection for one week, and a renal I/R injury model was constructed. HK-2 cells were incubated with puerarin (1 uM and 10 uM) before the H/R model. Immunohistochemistry, immunocytochemistry, and Western blot analysis were used to detect the protein associated with apoptosis and endoplasmic reticulum stress.Results: Puerarin could improve renal function and attenuate tissue structural damage after renal I/R. Meanwhile, puerarin alleviated apoptosis and endoplasmic reticulum stress by decreasing expression levels of specific biomarkers such as caspase-3, GRP78, CHOP, and p-elF2α/ elF2α in animals and HK-2 cells. The up-regulated expression of Nrf2 and HO-1 protein after puerarin treatment indicated that the Nrf2/HO-1 signaling pathway might mediate the protective mechanism of puerarin against renal I/R.Conclusion: Our results suggest that puerarin alleviated renal ischemia/reperfusion injury by inhibiting apoptosis and endoplasmic reticulum stress via the Nrf2/HO-1 pathway and offered new insights for preventing and treating renal I/R.","PeriodicalId":14495,"journal":{"name":"Iranian Journal of Basic Medical Sciences","volume":"28 2","pages":"187-193"},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11756738/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143028464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chronic stress-induced anxiety-like behavior, hippocampal oxidative, and endoplasmic reticulum stress are reversed by young plasma transfusion in aged adult rats. 输注年轻血浆可逆转老年成年大鼠由慢性应激诱发的焦虑样行为、海马氧化应激和内质网应激。

IF 2.2 4区医学

Iranian Journal of Basic Medical Sciences Pub Date : 2024-01-01 DOI: 10.22038/IJBMS.2023.72437.15754

Arshad Ghaffari-Nasab, Gonja Javani, Fereshteh Farajdokht, Mohammad Reza Alipour, Gisou Mohaddes

{"title":"Chronic stress-induced anxiety-like behavior, hippocampal oxidative, and endoplasmic reticulum stress are reversed by young plasma transfusion in aged adult rats.","authors":"Arshad Ghaffari-Nasab, Gonja Javani, Fereshteh Farajdokht, Mohammad Reza Alipour, Gisou Mohaddes","doi":"10.22038/IJBMS.2023.72437.15754","DOIUrl":"10.22038/IJBMS.2023.72437.15754","url":null,"abstract":"Objectives: Aging and stress synergistically induce behavioral dysfunctions associated with oxidative and endoplasmic reticulum (ER) stress in brain regions. Considering the rejuvenating effects of young plasma on aging brain function, in the current study, we examined the effects of young plasma administration on anxiety-like behavior, NADH oxidase, NADPH oxidase, and ER stress markers in the hippocampus of old male rats.Materials and methods: Young (3 months old) and aged (22 months old) rats were randomly assigned into five groups: young control (Y), aged control (A), aged rats subjected to chronic stress for four weeks (A+S), aged rats subjected to chronic stress and treated with old plasma (A+S+OP), and aged rats subjected to chronic stress and treated with young plasma (A+S+YP). Systemic injection of (1 ml) young and old plasma was performed for four weeks (3 times/week).Results: Young plasma transfusion significantly improved anxiety-like behavior in aged rats and modulated oxidative stress in the hippocampus, evidenced by the increased NADH oxidase (NOX) activity and the reduced NADPH oxidase. In addition, the levels of C/EBP homologous protein (CHOP) and Glucose-Regulated Protein 78 (GRP-78), as ER stress markers, markedly reduced in the hippocampus following the administration of young plasma.Conclusion: These findings suggest that young plasma transfusion could reverse anxiety-like behavior in stress-exposed aged rats by modulating the hippocampal oxidative and ER stress markers.","PeriodicalId":14495,"journal":{"name":"Iranian Journal of Basic Medical Sciences","volume":"27 1","pages":"114-121"},"PeriodicalIF":2.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10722476/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139074066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Crocin protects against endoplasmic reticulum stress-related tubular injury in diabetic nephropathy via the activation of the PI3K/AKT/Nrf2 pathway. 藏红花苷可通过激活 PI3K/AKT/Nrf2 通路防止糖尿病肾病中与内质网应激相关的肾小管损伤。

IF 2.2 4区医学

Iranian Journal of Basic Medical Sciences Pub Date : 2024-01-01 DOI: 10.22038/IJBMS.2023.73385.15942

Guiying Wang, Jiuhon Deng, Zhou Hua

{"title":"Crocin protects against endoplasmic reticulum stress-related tubular injury in diabetic nephropathy via the activation of the PI3K/AKT/Nrf2 pathway.","authors":"Guiying Wang, Jiuhon Deng, Zhou Hua","doi":"10.22038/IJBMS.2023.73385.15942","DOIUrl":"10.22038/IJBMS.2023.73385.15942","url":null,"abstract":"Objectives: Diabetic nephropathy (DN) is the main cause of end-stage renal disease, but the current treatment is not satisfactory. Crocin is a major bioactive compound of saffron with antioxidant and anti-endoplasmic reticulum stress (ERS) abilities used to treat diabetes. This study specifically investigated whether crocin has a regulatory role in renal injury in DN.Materials and methods: The experiment was divided into control, (db/m mice), model (db/db mice), and experimental groups (db/db mice were intraperitoneally injected with 40 mg/kg crocin). Renal function-related indicators (Scr, BUN, FBG, UP, TG, TC, ALT, and AST) and oxidative stress-related indicators (ROS, MDA, GSH, SOD, and CAT) were assessed. The pathological changes of renal tissues were confirmed by HE, Masson, PAS, and TUNEL staining. The levels of ERS-related proteins (GRP78 and CHOP), apoptosis-related proteins, and PI3K/AKT and Nrf2 pathways-related proteins in renal tissue were detected.Results: In db/db mice, renal function-related indicators, apoptotic cells of renal tissues, the contents of ROS and MDA as well as the expressions of CHOP, GRP78, and Bax were increased, the degree of renal tissue damage was aggravated, while the contents of GSH, SOD, and CAT, as well as the protein levels of Nrf2, PARP, anti-apoptotic proteins (Mcl-1, Bcl-2, Bcl-xl) were decreased compared to the db/m mice. However, crocin treatment reversed the above-mentioned situation. The expressions of the PI3K/AKT and Nrf2 pathways-related proteins were also activated by crocin.Conclusion: Crocin inhibited oxidative stress and ERS-induced kidney injury in db/db mice by activating the PI3K/AKT and Nrf2 pathways.","PeriodicalId":14495,"journal":{"name":"Iranian Journal of Basic Medical Sciences","volume":"27 4","pages":"439-446"},"PeriodicalIF":2.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10897564/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139990051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Orexin and cannabinoid systems modulate long-term potentiation of the hippocampus CA1 area in anesthetized rats. 奥列克辛和大麻素系统调节麻醉大鼠海马 CA1 区的长期电位。

IF 2.2 4区医学

Iranian Journal of Basic Medical Sciences Pub Date : 2024-01-01 DOI: 10.22038/IJBMS.2023.73979.16075

Reza Fartootzadeh, Ramezan Ali Taheri, Mohammad Reza Nourani, Kamelya Goudarzi

{"title":"Orexin and cannabinoid systems modulate long-term potentiation of the hippocampus CA1 area in anesthetized rats.","authors":"Reza Fartootzadeh, Ramezan Ali Taheri, Mohammad Reza Nourani, Kamelya Goudarzi","doi":"10.22038/IJBMS.2023.73979.16075","DOIUrl":"10.22038/IJBMS.2023.73979.16075","url":null,"abstract":"Objectives: Long-term potentiation (LTP) is a kind of synaptic plasticity and has a key role in learning and memory. Endocannabinoids and orexins are the endogenous systems that can modulate synaptic plasticity. Given that new studies have shown an interaction between cannabinoid and orexin systems in the brain, we decided to examine this interaction between the two systems on LTP induction in rat's hippocampus.Materials and methods: Twenty-eight male Wistar rats were used for evaluating the effects of co-administrating of cannabinoid-1 receptor (CB1R) antagonist (AM251) and orexin-2 receptor (OX2R) antagonist (TCS OX2 29) on the induction of LTP in the Schaffer collateral-CA1 synapses of rat hippocampus. The drugs were microinjected into the CA1 area of rat hippocampus 30 min before inducing of LTP.Results: Results showed that sole administration of the antagonists inhibited LTP, with respect to the control group. Also, co-administrating of them reduced LTP as compared to the control group, but not significantly more than that when the antagonists were solely microinjected into the CA1. Nonetheless, the inhibitory effect of concurrent administration of the antagonists on LTP lasted until the end of the recording.Conclusion: These results propose that endogenous cannabinoids and orexins play a role in the expression of LTP, at least by CA1-CB1Rs and CA1-OX2Rs, respectively. Finally, there is no interaction between CB1R and OX2R on the induction of LTP in the Schaffer collateral-CA1 synapses; therefore, these two systems possibly act through common signaling pathways in the hippocampus's CA1 region.","PeriodicalId":14495,"journal":{"name":"Iranian Journal of Basic Medical Sciences","volume":"27 4","pages":"461-465"},"PeriodicalIF":2.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10897562/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139990057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Medicinal potential of embelin and its nanoformulations: An update on the molecular mechanism and various applications. 栓皮素及其纳米制剂的药用潜力：分子机制和各种应用的最新进展。

IF 2.1 4区医学

Iranian Journal of Basic Medical Sciences Pub Date : 2024-01-01 DOI: 10.22038/ijbms.2024.77888.16850

Asad Ali, Nasr A Emad, Niha Sultana, Hamad Ali, Samreen Jahan, Mohd Aqil, Mohd Mujeeb, Yasmin Sultana

{"title":"Medicinal potential of embelin and its nanoformulations: An update on the molecular mechanism and various applications.","authors":"Asad Ali, Nasr A Emad, Niha Sultana, Hamad Ali, Samreen Jahan, Mohd Aqil, Mohd Mujeeb, Yasmin Sultana","doi":"10.22038/ijbms.2024.77888.16850","DOIUrl":"10.22038/ijbms.2024.77888.16850","url":null,"abstract":"Natural herbs have garnered significant research recently as their components target multiple disease signaling pathways, making them highly potential for various disease prevention and treatment. Embelin, a naturally occurring benzoquinone isolated from Embelia ribes, has shown promising biological activities such as antitumor, antidiabetic, anti-oxidant, and antimicrobial. Various mechanisms have been reported, including monitoring genes that synchronize the cell cycle, up-regulating multiple anti-oxidant enzymes, suppressing genes that prevent cell death, influencing transcription factors, and preventing inflammatory biomarkers. However, the hydrophobic nature of embelin leads to poor absorption and limits its therapeutic potential. This review highlights a wide range of nanocarriers used as delivery systems for embelin, including polymeric nanoparticles, liposomes, nanostructured lipid carriers, micelles, nanoemulsion, and metallic nanoparticles. These embelin nanomedicine formulations have been developed in preclinical studies as a possible treatment for many disorders and characterized using various in vitro, ex vivo, and in vivo models.","PeriodicalId":14495,"journal":{"name":"Iranian Journal of Basic Medical Sciences","volume":"27 10","pages":"1228-1242"},"PeriodicalIF":2.1,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11366951/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142125701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0