The mTOR pathway is involved in the process of platelet-rich plasma improving intervertebral disc degeneration.

IF 2.1 4区 医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL
Jing Luan, Qi Wang, Wei Zheng, Yongjin He
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引用次数: 0

Abstract

Objectives: Platelet-rich plasma (PRP) contains multiple growth hormones that may stimulate tissue repair. We aimed to assess PRP's efficacy and explore possible mechanisms using the intervertebral disc degeneration (IDD) model.

Materials and methods: A total of 48 male Sprague-Dawley (SD) rats were randomly divided into three groups: sham, IDD+PBS, and IDD+PRP (n=16, respectively). IL-1β (10 ng/ml) was used to establish a humanized IDD model in human lumbar nucleus pulposus (NP) tissues from 36 patients with degenerative disc disease. These NP cells were randomly divided into three groups: sham, IDD+PBS, and IDD+PRP (n=12, respectively). RT-PCR and western blot were used to detect the expression of aggrecan, collagen II, IL-1β, IL-6, TNF-α, Bcl-2, cleaved-Caspase 3, Bax and Akt/mTOR/p70S6K signaling pathway. A related assay kit was used to detect MDA, SOD, and GSH.

Results: PRP affected the expression of aggrecan, collagen II, IL-1β, IL-6, TNF-α, MDA, SOD, GSH, Bcl-2, cleaved-Caspase 3, and Bax in IDD rats. Compared with the IDD+PBS group, the expression of p-mTOR, p-p70/S6K, and p-Akt was much lower in the rat IDD+PRP group (P<0.05). Similarly, with PRP treatment in the humanized IDD model, the expression of p-mTOR, p-p70/S6K, and p-Akt was also inhibited.

Conclusion: PRP may be a potential therapy for IDD via the mTOR signaling pathway in regulating and affecting extracellular matrix degradation, inflammatory factors, oxidative stress, and apoptosis.

mTOR通路参与富血小板血浆改善椎间盘退变的过程。
目的:富血小板血浆(PRP)含有多种生长激素,可刺激组织修复。我们的目的是评估PRP的疗效,并利用椎间盘退变(IDD)模型探讨可能的机制。材料与方法:雄性SD大鼠48只,随机分为假手术组、IDD+PBS组和IDD+PRP组(n=16)。采用IL-1β (10 ng/ml)在36例退行性椎间盘病患者腰椎髓核(NP)组织中建立人源性IDD模型。将这些NP细胞随机分为sham、IDD+PBS和IDD+PRP三组(n=12)。采用RT-PCR和western blot检测aggrecan、collagen II、IL-1β、IL-6、TNF-α、Bcl-2、cleaved-Caspase 3、Bax和Akt/mTOR/p70S6K信号通路的表达。采用相关检测试剂盒检测MDA、SOD、GSH。结果:PRP影响IDD大鼠聚集蛋白、II型胶原、IL-1β、IL-6、TNF-α、MDA、SOD、GSH、Bcl-2、cleaved-Caspase 3、Bax的表达。与IDD+PBS组比较,IDD+PRP组大鼠p-mTOR、p-p70/S6K、p-Akt的表达明显降低(P0.05)。同样,在人源性IDD模型中,PRP处理也抑制了p-mTOR、p-p70/S6K和p-Akt的表达。结论:PRP可能通过mTOR信号通路调控和影响细胞外基质降解、炎症因子、氧化应激和细胞凋亡,是治疗IDD的一种潜在疗法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Iranian Journal of Basic Medical Sciences
Iranian Journal of Basic Medical Sciences MEDICINE, RESEARCH & EXPERIMENTAL-PHARMACOLOGY & PHARMACY
CiteScore
4.00
自引率
4.50%
发文量
142
审稿时长
6-12 weeks
期刊介绍: The Iranian Journal of Basic Medical Sciences (IJBMS) is a peer-reviewed, monthly publication by Mashhad University of Medical Sciences (MUMS), Mashhad, Iran . The Journal of "IJBMS” is a modern forum for scientific communication. Data and information, useful to investigators in any discipline in basic medical sciences mainly including Anatomical Sciences, Biochemistry, Genetics, Immunology, Microbiology, Pathology, Pharmacology, Pharmaceutical Sciences, and Physiology, will be published after they have been peer reviewed. This will also include reviews and multidisciplinary research.
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