Iranian Journal of Basic Medical Sciences最新文献

Chronic stress-induced anxiety-like behavior, hippocampal oxidative, and endoplasmic reticulum stress are reversed by young plasma transfusion in aged adult rats. 输注年轻血浆可逆转老年成年大鼠由慢性应激诱发的焦虑样行为、海马氧化应激和内质网应激。

IF 2.2 4区医学

Iranian Journal of Basic Medical Sciences Pub Date : 2024-01-01 DOI: 10.22038/IJBMS.2023.72437.15754

Arshad Ghaffari-Nasab, Gonja Javani, Fereshteh Farajdokht, Mohammad Reza Alipour, Gisou Mohaddes

{"title":"Chronic stress-induced anxiety-like behavior, hippocampal oxidative, and endoplasmic reticulum stress are reversed by young plasma transfusion in aged adult rats.","authors":"Arshad Ghaffari-Nasab, Gonja Javani, Fereshteh Farajdokht, Mohammad Reza Alipour, Gisou Mohaddes","doi":"10.22038/IJBMS.2023.72437.15754","DOIUrl":"10.22038/IJBMS.2023.72437.15754","url":null,"abstract":"Objectives: Aging and stress synergistically induce behavioral dysfunctions associated with oxidative and endoplasmic reticulum (ER) stress in brain regions. Considering the rejuvenating effects of young plasma on aging brain function, in the current study, we examined the effects of young plasma administration on anxiety-like behavior, NADH oxidase, NADPH oxidase, and ER stress markers in the hippocampus of old male rats.Materials and methods: Young (3 months old) and aged (22 months old) rats were randomly assigned into five groups: young control (Y), aged control (A), aged rats subjected to chronic stress for four weeks (A+S), aged rats subjected to chronic stress and treated with old plasma (A+S+OP), and aged rats subjected to chronic stress and treated with young plasma (A+S+YP). Systemic injection of (1 ml) young and old plasma was performed for four weeks (3 times/week).Results: Young plasma transfusion significantly improved anxiety-like behavior in aged rats and modulated oxidative stress in the hippocampus, evidenced by the increased NADH oxidase (NOX) activity and the reduced NADPH oxidase. In addition, the levels of C/EBP homologous protein (CHOP) and Glucose-Regulated Protein 78 (GRP-78), as ER stress markers, markedly reduced in the hippocampus following the administration of young plasma.Conclusion: These findings suggest that young plasma transfusion could reverse anxiety-like behavior in stress-exposed aged rats by modulating the hippocampal oxidative and ER stress markers.","PeriodicalId":14495,"journal":{"name":"Iranian Journal of Basic Medical Sciences","volume":"27 1","pages":"114-121"},"PeriodicalIF":2.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10722476/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139074066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Crocin protects against endoplasmic reticulum stress-related tubular injury in diabetic nephropathy via the activation of the PI3K/AKT/Nrf2 pathway. 藏红花苷可通过激活 PI3K/AKT/Nrf2 通路防止糖尿病肾病中与内质网应激相关的肾小管损伤。

IF 2.2 4区医学

Iranian Journal of Basic Medical Sciences Pub Date : 2024-01-01 DOI: 10.22038/IJBMS.2023.73385.15942

Guiying Wang, Jiuhon Deng, Zhou Hua

{"title":"Crocin protects against endoplasmic reticulum stress-related tubular injury in diabetic nephropathy via the activation of the PI3K/AKT/Nrf2 pathway.","authors":"Guiying Wang, Jiuhon Deng, Zhou Hua","doi":"10.22038/IJBMS.2023.73385.15942","DOIUrl":"10.22038/IJBMS.2023.73385.15942","url":null,"abstract":"Objectives: Diabetic nephropathy (DN) is the main cause of end-stage renal disease, but the current treatment is not satisfactory. Crocin is a major bioactive compound of saffron with antioxidant and anti-endoplasmic reticulum stress (ERS) abilities used to treat diabetes. This study specifically investigated whether crocin has a regulatory role in renal injury in DN.Materials and methods: The experiment was divided into control, (db/m mice), model (db/db mice), and experimental groups (db/db mice were intraperitoneally injected with 40 mg/kg crocin). Renal function-related indicators (Scr, BUN, FBG, UP, TG, TC, ALT, and AST) and oxidative stress-related indicators (ROS, MDA, GSH, SOD, and CAT) were assessed. The pathological changes of renal tissues were confirmed by HE, Masson, PAS, and TUNEL staining. The levels of ERS-related proteins (GRP78 and CHOP), apoptosis-related proteins, and PI3K/AKT and Nrf2 pathways-related proteins in renal tissue were detected.Results: In db/db mice, renal function-related indicators, apoptotic cells of renal tissues, the contents of ROS and MDA as well as the expressions of CHOP, GRP78, and Bax were increased, the degree of renal tissue damage was aggravated, while the contents of GSH, SOD, and CAT, as well as the protein levels of Nrf2, PARP, anti-apoptotic proteins (Mcl-1, Bcl-2, Bcl-xl) were decreased compared to the db/m mice. However, crocin treatment reversed the above-mentioned situation. The expressions of the PI3K/AKT and Nrf2 pathways-related proteins were also activated by crocin.Conclusion: Crocin inhibited oxidative stress and ERS-induced kidney injury in db/db mice by activating the PI3K/AKT and Nrf2 pathways.","PeriodicalId":14495,"journal":{"name":"Iranian Journal of Basic Medical Sciences","volume":"27 4","pages":"439-446"},"PeriodicalIF":2.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10897564/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139990051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Orexin and cannabinoid systems modulate long-term potentiation of the hippocampus CA1 area in anesthetized rats. 奥列克辛和大麻素系统调节麻醉大鼠海马 CA1 区的长期电位。

IF 2.2 4区医学

Iranian Journal of Basic Medical Sciences Pub Date : 2024-01-01 DOI: 10.22038/IJBMS.2023.73979.16075

Reza Fartootzadeh, Ramezan Ali Taheri, Mohammad Reza Nourani, Kamelya Goudarzi

{"title":"Orexin and cannabinoid systems modulate long-term potentiation of the hippocampus CA1 area in anesthetized rats.","authors":"Reza Fartootzadeh, Ramezan Ali Taheri, Mohammad Reza Nourani, Kamelya Goudarzi","doi":"10.22038/IJBMS.2023.73979.16075","DOIUrl":"10.22038/IJBMS.2023.73979.16075","url":null,"abstract":"Objectives: Long-term potentiation (LTP) is a kind of synaptic plasticity and has a key role in learning and memory. Endocannabinoids and orexins are the endogenous systems that can modulate synaptic plasticity. Given that new studies have shown an interaction between cannabinoid and orexin systems in the brain, we decided to examine this interaction between the two systems on LTP induction in rat's hippocampus.Materials and methods: Twenty-eight male Wistar rats were used for evaluating the effects of co-administrating of cannabinoid-1 receptor (CB1R) antagonist (AM251) and orexin-2 receptor (OX2R) antagonist (TCS OX2 29) on the induction of LTP in the Schaffer collateral-CA1 synapses of rat hippocampus. The drugs were microinjected into the CA1 area of rat hippocampus 30 min before inducing of LTP.Results: Results showed that sole administration of the antagonists inhibited LTP, with respect to the control group. Also, co-administrating of them reduced LTP as compared to the control group, but not significantly more than that when the antagonists were solely microinjected into the CA1. Nonetheless, the inhibitory effect of concurrent administration of the antagonists on LTP lasted until the end of the recording.Conclusion: These results propose that endogenous cannabinoids and orexins play a role in the expression of LTP, at least by CA1-CB1Rs and CA1-OX2Rs, respectively. Finally, there is no interaction between CB1R and OX2R on the induction of LTP in the Schaffer collateral-CA1 synapses; therefore, these two systems possibly act through common signaling pathways in the hippocampus's CA1 region.","PeriodicalId":14495,"journal":{"name":"Iranian Journal of Basic Medical Sciences","volume":"27 4","pages":"461-465"},"PeriodicalIF":2.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10897562/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139990057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Medicinal potential of embelin and its nanoformulations: An update on the molecular mechanism and various applications. 栓皮素及其纳米制剂的药用潜力：分子机制和各种应用的最新进展。

IF 2.1 4区医学

Iranian Journal of Basic Medical Sciences Pub Date : 2024-01-01 DOI: 10.22038/ijbms.2024.77888.16850

Asad Ali, Nasr A Emad, Niha Sultana, Hamad Ali, Samreen Jahan, Mohd Aqil, Mohd Mujeeb, Yasmin Sultana

{"title":"Medicinal potential of embelin and its nanoformulations: An update on the molecular mechanism and various applications.","authors":"Asad Ali, Nasr A Emad, Niha Sultana, Hamad Ali, Samreen Jahan, Mohd Aqil, Mohd Mujeeb, Yasmin Sultana","doi":"10.22038/ijbms.2024.77888.16850","DOIUrl":"10.22038/ijbms.2024.77888.16850","url":null,"abstract":"Natural herbs have garnered significant research recently as their components target multiple disease signaling pathways, making them highly potential for various disease prevention and treatment. Embelin, a naturally occurring benzoquinone isolated from Embelia ribes, has shown promising biological activities such as antitumor, antidiabetic, anti-oxidant, and antimicrobial. Various mechanisms have been reported, including monitoring genes that synchronize the cell cycle, up-regulating multiple anti-oxidant enzymes, suppressing genes that prevent cell death, influencing transcription factors, and preventing inflammatory biomarkers. However, the hydrophobic nature of embelin leads to poor absorption and limits its therapeutic potential. This review highlights a wide range of nanocarriers used as delivery systems for embelin, including polymeric nanoparticles, liposomes, nanostructured lipid carriers, micelles, nanoemulsion, and metallic nanoparticles. These embelin nanomedicine formulations have been developed in preclinical studies as a possible treatment for many disorders and characterized using various in vitro, ex vivo, and in vivo models.","PeriodicalId":14495,"journal":{"name":"Iranian Journal of Basic Medical Sciences","volume":"27 10","pages":"1228-1242"},"PeriodicalIF":2.1,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11366951/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142125701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advancements in the role of phytochemicals and medicinal plants in prophylaxis and management of Alzheimer's disease. 植物化学物质和药用植物在预防和治疗阿尔茨海默病方面作用的最新进展。

IF 2.1 4区医学

Iranian Journal of Basic Medical Sciences Pub Date : 2024-01-01 DOI: 10.22038/ijbms.2024.77760.16826

Akanksha Mishra, Sairam Krishnamurthy

{"title":"Recent advancements in the role of phytochemicals and medicinal plants in prophylaxis and management of Alzheimer's disease.","authors":"Akanksha Mishra, Sairam Krishnamurthy","doi":"10.22038/ijbms.2024.77760.16826","DOIUrl":"10.22038/ijbms.2024.77760.16826","url":null,"abstract":"Medicinal plants and phytochemicals are some of the major sources in the treatment of various neurodegenerative disorders including Alzheimer's disease (AD). There is no FDA-approved drug to target AD pathology directly. Full cognitive restoration and management of psychosis-like symptoms are still to be achieved. Being comparatively safer with fewer side effects, medicinal plants have been among the major areas of interest to be researched. Several mechanistic pathways are involved in AD including anticholinesterase activity, glutamate toxicity, free radicals generation, Amyloid β (Aβ) toxicity, inflammation, and mitochondrial dysfunction. Various phytochemicals such as paenol, andrographolide, isoquercitrin, flavonoids, and saponins obtained from different plant sources, various medicinal plants like Spirulina maxima, Salicornia europaea, Curcuma longa, Citrus Junos Tanaka, Cassiae semen, Centella asiatica as well as various traditional medicinal plants of China, Asia, Europe, Turkey, and Iran have been found effective against one or more of these targets. Large numbers of clinical trials are under process to evaluate the role of different phytoconstituents in AD management. Out of 143 agents under clinical trials, 119 have been categorized as disease-modifying agents. The present review extensively covers the recent advancements in the usage of phytochemicals and medicinal plants in various experimental AD models. It involves clinical trials and other research works divided into three sections, including those performed in vitro, in vivo, and in humans mainly from the last five years along with disease markers and mechanistic pathways involved. However, phytochemicals should be explored further in order to achieve neurorestoration in AD.","PeriodicalId":14495,"journal":{"name":"Iranian Journal of Basic Medical Sciences","volume":"27 11","pages":"1357-1369"},"PeriodicalIF":2.1,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11459346/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142390542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Saroglitazar suppresses KIM-1 and type IV collagen in high fat diet and low-dose streptozotocin-induced diabetic nephropathy in Wistar rats. 沙格列扎抑制高脂饮食和低剂量链脲佐菌素诱导的 Wistar 大鼠糖尿病肾病中的 KIM-1 和 IV 型胶原蛋白。

IF 2.1 4区医学

Iranian Journal of Basic Medical Sciences Pub Date : 2024-01-01 DOI: 10.22038/ijbms.2024.78221.16908

Rizwan Ahamad, Uma Bhandari, Sayima Nabi, Shweta Sharma

{"title":"Saroglitazar suppresses KIM-1 and type IV collagen in high fat diet and low-dose streptozotocin-induced diabetic nephropathy in Wistar rats.","authors":"Rizwan Ahamad, Uma Bhandari, Sayima Nabi, Shweta Sharma","doi":"10.22038/ijbms.2024.78221.16908","DOIUrl":"10.22038/ijbms.2024.78221.16908","url":null,"abstract":"Objectives: Nephropathy is the most common comorbidity linked to T2D. The present study aimed to examine the potential of saroglitazar in the context of a high-fat diet and low-dose streptozotocin-induced diabetic nephropathy in Wistar rats.Materials and methods: Molecular docking simulation investigations were conducted on the ligand-binding region of type IV collagen and Kidney injury molecule-1 (KIM-1), using saroglitazar and fenofibrate as the subjects. The rats were fed either a conventional rodent diet or a high-fat diet ad libitum for two weeks. Following a two-week period, the rats given an HFD were administered with a low-dose of STZ (35 mg/kg, IP). Rats with experimentally induced diabetes were categorized into five groups: normal control; diabetic control; HFD+STZ+saroglitazar (2 mg/kg); HFD+STZ+saroglitazar (4 mg/kg); HFD+STZ+fenofibrate (100 mg/kg) treated orally for 21 days with continuation on HFD. After 21 days, rats were kept on fasting overnight, blood and urine was acquired for various biochemical analysis. Animals were sacrificed, and kidney tissues were removed for histopathological studies.Results: In-silico investigation showed a substantial affinity between saroglitazar and fenofibrate with KIM-1 and type IV collagen. Saroglitazar produced a significant (P<0.01) reduction in weight of the body, serum blood sugar, albumin, creatinine, and BUN levels. Further, saroglitazar significantly (P<0.01) reduced the KIM-1 and type IV collagen levels in the urine of diabetic rats. Histopathological results showed improvement in tubular degeneration, necrosis, and dilatation of Bowman's space in kidney tissue.Conclusion: Saroglitazar attenuated renal injury by improving renal function in HFD+STZ-induced DN in Wistar rats.","PeriodicalId":14495,"journal":{"name":"Iranian Journal of Basic Medical Sciences","volume":"27 11","pages":"1447-1455"},"PeriodicalIF":2.1,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11459336/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142390543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TRPA1 as a promising target in ischemia/reperfusion: A comprehensive review. TRPA1是缺血/再灌注的一个有希望的靶点：综述。

IF 2.2 4区医学

Iranian Journal of Basic Medical Sciences Pub Date : 2024-01-01 DOI: 10.22038/IJBMS.2023.74590.16198

Azin Alizadehasl, Maryam Sadat Alavi, Mohaddeseh Sadat Alavi, Ali Roohbakhsh

{"title":"TRPA1 as a promising target in ischemia/reperfusion: A comprehensive review.","authors":"Azin Alizadehasl, Maryam Sadat Alavi, Mohaddeseh Sadat Alavi, Ali Roohbakhsh","doi":"10.22038/IJBMS.2023.74590.16198","DOIUrl":"10.22038/IJBMS.2023.74590.16198","url":null,"abstract":"Ischemic disorders, including myocardial infarction, cerebral ischemia, and peripheral vascular impairment, are the main common reasons for debilitating diseases and death in Western cultures. Ischemia occurs when blood circulation is reduced in tissues. Reperfusion, although commanded to return oxygen to ischemic tissues, generates paradoxical tissue responses. The responses include generating reactive oxygen species (ROS), stimulating inflammatory responses in ischemic organs, endoplasmic reticulum stress, and the expansion of postischemic capillary no-reflow, which intensifies organ damage. Multiple pathologic processes contribute to ischemia/reperfusion; therefore, targeting different pathologic processes may yield an effective therapeutic approach. Transient Receptor Potential A1 (TRPA1) belongs to the TRP family of ion channels, detects a broad range of chemicals, and promotes the transduction of noxious stimuli, e.g., methylglyoxal, ROS, and acrolein effects are attributed to the channel's sensitivity to intracellular calcium elevation or phosphoinositol phosphate modulation. Hypoxia and ischemia are associated with oxidative stress, which activates the TRPA1 channel. This review describes the role of TRPA1 and its related mechanisms that contribute to ischemia/reperfusion. Relevant articles were searched from PubMed, Scopus, Web of Sciences, and Google Scholar electronic databases, up to the end of August 2023. Based on the evidence presented here, TRPA1 may have protective or deteriorative functions during the ischemia/reperfusion process. Its function depends on the activation level, the ischemic region, the extent of lesions, and the duration of ischemia.","PeriodicalId":14495,"journal":{"name":"Iranian Journal of Basic Medical Sciences","volume":"27 3","pages":"270-278"},"PeriodicalIF":2.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10849207/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139706745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neuroprotective roles of flavonoid "hispidulin" in the central nervous system: A review. 黄酮类化合物 "hispidulin "在中枢神经系统中的神经保护作用：综述。

IF 2.1 4区医学

Iranian Journal of Basic Medical Sciences Pub Date : 2024-01-01 DOI: 10.22038/IJBMS.2024.76605.16573

Saeed Mustapha, Rabiu Abdussalam Magaji, Mohammed Garba Magaji, Ibrahim Bako Gaya, Baraka Umar, Yusuf Yusha'u, Abubakar Bishir Daku, Samaila Musa Chiroma, Aliyu Jaafar, Mohamad Zulfadli Mehat, Che Norma Mat Taib, Mohamad Aris Moh'd Moklas

{"title":"Neuroprotective roles of flavonoid \"hispidulin\" in the central nervous system: A review.","authors":"Saeed Mustapha, Rabiu Abdussalam Magaji, Mohammed Garba Magaji, Ibrahim Bako Gaya, Baraka Umar, Yusuf Yusha'u, Abubakar Bishir Daku, Samaila Musa Chiroma, Aliyu Jaafar, Mohamad Zulfadli Mehat, Che Norma Mat Taib, Mohamad Aris Moh'd Moklas","doi":"10.22038/IJBMS.2024.76605.16573","DOIUrl":"10.22038/IJBMS.2024.76605.16573","url":null,"abstract":"Interest in naturally occurring phytochemicals has been on the increase, they are believed to reduce the risk of brain disorders. Hispidulin (HN) is a phenolic flavonoid compound with various pharmacological and biological effects on the central nervous system. It belongs to the flavone class of flavonoids. It can be found in different plant materials, especially fruits and vegetables. The literature used in this review was collected from credible scientific databases including ScienceDirect, Scopus, PubMed, Google Scholar, and Hindawi without time restriction, using relevant keywords, such as HN, brain, central nervous system, flavonoids, and flavones. HN was discovered to possess pro-apoptotic properties, act as an antioxidant, inhibit cytokine production and toll-like receptor 4 expression, as well as impede nuclear factor kappa beta and mitogen-activated protein kinase B. HN was also found to inhibit lipid peroxidation in vitro and reduce brain edema in mice. These pharmacological potentials suggest that HN is a promising candidate for neuroprotection in CNS disorders like depression and epilepsy. This review provides an update on the scientific literature concerning how these activities could help provide various forms of neuroprotection in the CNS. Additional experimental data on the effects of HN in models of neurological disorders and neuroprotection should be explored further. Based on the current study, HN is a promising candidate for neuroprotection of the CNS.","PeriodicalId":14495,"journal":{"name":"Iranian Journal of Basic Medical Sciences","volume":"27 9","pages":"1077-1084"},"PeriodicalIF":2.1,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11266747/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141758747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Crocus sativus and its constituent, safranal, and pioglitazone, on systemic inflammation and oxidative stress induced by paraquat aerosol in rats. 番泻叶及其成分沙呋醛和吡格列酮对百草枯气溶胶诱导的大鼠全身炎症和氧化应激的影响

IF 2.2 4区医学

Iranian Journal of Basic Medical Sciences Pub Date : 2024-01-01 DOI: 10.22038/IJBMS.2024.72996.15867

Arghavan Memarzia, Seydeh Zahra Ghasemi, Fatemeh Amin, Zahra Gholamnezhad, Mohammad Hossein Boskabady

{"title":"Effects of Crocus sativus and its constituent, safranal, and pioglitazone, on systemic inflammation and oxidative stress induced by paraquat aerosol in rats.","authors":"Arghavan Memarzia, Seydeh Zahra Ghasemi, Fatemeh Amin, Zahra Gholamnezhad, Mohammad Hossein Boskabady","doi":"10.22038/IJBMS.2024.72996.15867","DOIUrl":"https://doi.org/10.22038/IJBMS.2024.72996.15867","url":null,"abstract":"Objectives: The effects of Crocus sativus, safranal, and pioglitazone on aerosolized paraquat (PQ)-induced systemic changes were examined.Materials and methods: Control (Ctrl) and PQ groups of rats were exposed to saline or PQ (27 and 54 mg/m3, PQ-L and PQ-H) aerosols eight times on alternate days. Nine PQ-H groups were treated with dexamethasone (0.03 mg/kg/day, Dexa), two doses of C. sativus extract (20 and 80 mg/kg/day, CS-L and CS-H), safranal (0.8 and 3.2 mg/kg/day, Saf-L and Saf-H), pioglitazone (5 and 10 mg/kg/day, Pio-L and Pio-H), and the combination of low dose of the pioglitazone and extract or safranal (Pio + CS and Pio + Saf) after the end of PQ exposure.Results: Interferon-gamma (INF-γ), interleukin 10 (IL-10), superoxide dismutase (SOD), catalase (CAT), and thiol serum levels were reduced, but tumor necrosis factor (TNF-α), malondialdehyde (MDA), and total and differential WBC were increased in both PQ groups (P<0.05 to P<0.001). All measured variables were improved in all treated groups (P<0.05 to P<0.001). The effects of high dose of C. sativus and safranal on measured parameters were higher than dexamethasone (P<0.05 to P<0.001). The effects of Pio + CS and Pio + Saf treatment on most variables were significantly higher than three agents alone (P<0.05 to P<0.001).Conclusion: C. sativus and safranal improved inhaled PQ-induced systemic inflammation and oxidative stress similar to those of dexamethasone and showed synergic effects with pioglitazone suggesting the possible PPARγ receptor-mediated effects of the plant and its constituent.","PeriodicalId":14495,"journal":{"name":"Iranian Journal of Basic Medical Sciences","volume":"27 5","pages":"640-646"},"PeriodicalIF":2.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11017852/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140860855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential antiaging activity of secretome gel of human Wharton's jelly mesenchymal stem cells (hWJ-MSCs) in UV-induced mice models. 人沃顿果冻间充质干细胞（hWJ-MSCs）分泌物凝胶在紫外线诱导的小鼠模型中的潜在抗衰老活性。

IF 2.2 4区医学

Iranian Journal of Basic Medical Sciences Pub Date : 2024-01-01 DOI: 10.22038/IJBMS.2024.70825.15385

Wahyu Widowati, Ahmad Faried, Achmad Adam, Deni Rahmat, Hanna Sari Widya Kusuma, Nindia Salsabila Mia Dewi, Marisca Evalina Gondokesumo, Rizal Rizal, Ita Margaretha Nainggolan, Massoud Vosough

{"title":"Potential antiaging activity of secretome gel of human Wharton's jelly mesenchymal stem cells (hWJ-MSCs) in UV-induced mice models.","authors":"Wahyu Widowati, Ahmad Faried, Achmad Adam, Deni Rahmat, Hanna Sari Widya Kusuma, Nindia Salsabila Mia Dewi, Marisca Evalina Gondokesumo, Rizal Rizal, Ita Margaretha Nainggolan, Massoud Vosough","doi":"10.22038/IJBMS.2024.70825.15385","DOIUrl":"10.22038/IJBMS.2024.70825.15385","url":null,"abstract":"Objectives: Skin aging is a degenerative process that can be induced by UV irradiation. UV radiation can produce reactive oxidate stress which causes premature aging. This study aims to examine the antiaging potential of secretome gel (SC) from human Wharton Jelly Mesenchymal Stem Cells (hWJ-MSCs) in a UVB-induced mice model.Materials and methods: The secretome was obtained from hWJ-MSCs and made in gel form. Male mice were radiated by UVB for 15 min twice daily for 14 days. The gel was topically applied to the mice's dorsal skin. Two treatments of secretome gel: secretome 1 is applied once and secretome 2 is applied twice daily after UVB radiation. TGF-β1, IL-10, and IL-18 gene expression was determined using RT-PCR. Hematoxylin Eosin staining was used to observe the inflammation and collagen density of skin tissue. An immunohistochemistry assay was used to analyze the protein expression of P53, COL4A1, MMP-2, and MMP-13. The data were statistically analyzed using the ANOVA test followed by the Tukey post hoc test (P<0.05).Results: UVB induction caused loss of collagen, increasing inflammation and high expression of aging mediators. SC increased the gene expression of TGF-β1 and IL-10 and decreased IL-18 gene expression. Histopathological tests showed that SG increased collagen density, lowered inflammation, and repaired cell damage in skin tissue. Immunohistochemistry test showed that SC decreased MMP-2, MMP-13, and P53 expression, in contrast, increased COL4A1.Conclusion: The secretome gel of hWJ-MSCs showed antiaging activities with potential for preventing and curing skin aging.","PeriodicalId":14495,"journal":{"name":"Iranian Journal of Basic Medical Sciences","volume":"27 7","pages":"868-878"},"PeriodicalIF":2.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11127088/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141154247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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