Iranian Journal of Basic Medical Sciences最新文献

{"title":"The effect of tert-butylhydroquinone on anxiolytic- and antidepressant-like behaviors induced by post-traumatic stress disorder: A behavioral and molecular study.","authors":"Samaneh Nabavi, Mohammad-Reza Zarrindast, Fariba Khodagholi, Mohammad Nasehi, Solmaz Khalifeh","doi":"10.22038/ijbms.2025.87679.18939","DOIUrl":"10.22038/ijbms.2025.87679.18939","url":null,"abstract":"<p><strong>Objectives: </strong>Post-traumatic stress disorder (PTSD) is a crippling mental illness that commonly co-occurs with anxiety and depression. Recent studies have established a link between neuroinflammation and the development of PTSD. Studies have revealed that tert-butylhydroquinone (tBHQ) exhibits anti-inflammatory effects. This research investigated the impact of tBHQ on the amelioration of PTSD-induced depression- and anxiety-like behaviors with respect to amygdala and hippocampal MAO-A, MAO-B, IL-6, IL-10, and glucocorticoid receptor in rats.</p><p><strong>Materials and methods: </strong>PTSD was triggered through the use of Single Prolonged Stress (SPS). The Elevated Plus Maze (EPM) and Forced Swim Test (FST) were employed to evaluate anxiety and depression, respectively. Protein assessment utilized Western blot assay for IL-6, IL-10, and the glucocorticoid receptor, in addition to enzyme-linked immunosorbent assay (ELISA) for MAO-A and MAO-B.</p><p><strong>Results: </strong>The data demonstrated that treatment with tBHQ ameliorates the depression- and anxiety-like behaviors in rats with PTSD. The ELISA findings indicated a rise in both MAO-A and B proteins in the hippocampus and amygdala due to PTSD, which was counteracted by a subthreshold amount of tBHQ. Additionally, the Western blot assay techniques revealed that PTSD led to an elevation in IL-6 levels, a pro-inflammatory cytokine, and a reduction in IL-10 levels, along with a decrease in glucocorticoid receptor expression in both brain regions, which was also counteracted by a subthreshold amount of tBHQ.</p><p><strong>Conclusion: </strong>These findings suggested that the beneficial effect of tBHQ on anxiety and depression induced by PTSD through hippocampal and amygdala MAO-A, MAO-B, IL-6, and IL-10, and glucocorticoid receptor.</p>","PeriodicalId":14495,"journal":{"name":"Iranian Journal of Basic Medical Sciences","volume":"29 1","pages":"113-120"},"PeriodicalIF":2.7,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12867118/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146119012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advances in the detection and treatment of hydatid cysts by nanomaterial-based carriers.","authors":"Nozhat Zebardast, Farid Ajori, Rahim Nosrati","doi":"10.22038/ijbms.2026.90995.19629","DOIUrl":"10.22038/ijbms.2026.90995.19629","url":null,"abstract":"<p><p>Cystic echinococcosis (CE) is a neglected parasitic disease. Although several therapeutic approaches are available for CE, including PAIR (Puncture, Aspiration, Injection, Re-aspiration), surgery, chemotherapy with anthelmintic drugs, and waiting for inactive cysts, these methods face challenges, and treatment choices remain debated. Regarding chemotherapy, especially with albendazole (ABZ) as the first-line drug, efficacy is limited by poor solubility and low bioavailability. Recently, nanoparticle (NP)-based systems, or nanocarriers, have attracted much interest in drug delivery, with ABZ as the main focus. This review summarizes the latest progress in developing diverse nanocarrier systems, such as liposomes, polymeric NPs (PNPs), and metallic NPs, highlighting their potential for scolicidal activity and for improving therapeutic approaches for CE. In addition, diagnostic methods, including imaging techniques and serological tests, as well as emerging nanotechnology-based approaches such as biosensors and nanosensors, are briefly discussed to provide a more comprehensive perspective on CE management. Subsequently, the prospects and challenges of drug-loaded nanocarriers for the treatment of CE are briefly discussed to further encourage their use as a means to improve drug delivery performance in CE therapy.</p>","PeriodicalId":14495,"journal":{"name":"Iranian Journal of Basic Medical Sciences","volume":"29 5","pages":"657-674"},"PeriodicalIF":3.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13254785/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148251061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LKB1 dictates sensitivity to immunotherapy through Skp2-mediated ubiquitination of immune checkpoint proteins in HCC with python analysis.","authors":"Masoud Khodarahmi, Danial Amiri Manjili, Foroozan Yarahmadi, Tahere Mokhtari, Adib Dashtizadeh, Helia Rajabi Dezfooli, Khaterehsadat Monirvaghefi, Hossein Gharedaghi, Sina Dolatshahi, Zahra Hasanabadi, Farzaneh Moammer, Mahya Mobinikhaledi, Aida Yavari Kondori, Zahra Farajpour, Pardis Kondori Varnosfaderani, Zahra Hashemi, Mohammad Amin Mahdizadeh, Qumars Behfar, Siamak Sandoghchian Shotorbani, Nasibeh Sargazi Moghaddam","doi":"10.22038/ijbms.2026.91840.19829","DOIUrl":"https://doi.org/10.22038/ijbms.2026.91840.19829","url":null,"abstract":"<p><strong>Objectives: </strong>Hepatocellular carcinoma (HCC) is a highly aggressive malignancy with limited treatment options, particularly in advanced stages. Immune checkpoint inhibitors (ICIs) targeting PD-1, PD-L1, and CTLA-4 have shown promise in cancer immunotherapy, but response rates in HCC remain variable.</p><p><strong>Materials and methods: </strong>Hep3B and HepG2 HCC cells were cultured and genetically manipulated to overexpress or deplete LKB1. Western blotting, real-time PCR, and immunofluorescence were used to assess PD-L1 expression at the protein and mRNA levels. The role of Skp2 in PD-L1 regulation was evaluated through shRNA-mediated knockdown and overexpression. Additionally, kinase-dead LKB1 mutants were expressed to determine the importance of LKB1 kinase activity in PD-L1 stability. ImageJ software and Python-based computational tools were employed for quantitative analysis of immunofluorescence and Western blot data.</p><p><strong>Results: </strong>LKB1 overexpression up-regulated PD-L1 protein levels in HCC cells, while its depletion reduced PD-L1 expression, indicating a post-translational regulatory mechanism. Although Skp2 expression remained unchanged upon LKB1 modulation, Skp2 overexpression in LKB1-deficient cells increased PD-L1 levels, suggesting a context-dependent role for Skp2 in PD-L1 stability. Furthermore, wild-type LKB1, but not the kinase-dead mutant, restored PD-L1 expression, highlighting the essential role of LKB1 kinase activity in PD-L1 regulation.</p><p><strong>Conclusion: </strong>This study identifies LKB1 as a critical regulator of PD-L1 stability in HCC, with implications for tumor immune evasion and immunotherapy response. While Skp2 appears to influence PD-L1 stability in specific contexts, LKB1's kinase activity is essential for PD-L1 regulation.</p>","PeriodicalId":14495,"journal":{"name":"Iranian Journal of Basic Medical Sciences","volume":"29 6","pages":"935-941"},"PeriodicalIF":3.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13480820/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148793223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resveratrol mitigates diazinon-induced neurotoxicity during fetal brain development.","authors":"Amir Delavar, Nasim Alipour, Javad Bagheri, Raheleh Baradaran, Alireza Seddigh, Elham Feridouni, Yousef Baghcheghi, Fatemeh Rahimi Anbarkeh","doi":"10.22038/ijbms.2026.94088.20276","DOIUrl":"10.22038/ijbms.2026.94088.20276","url":null,"abstract":"<p><strong>Objectives: </strong>Disruption of transcriptional programs governing fetal neurogenesis represents a critical but underexplored mechanism of diazinon (DZN)-induced developmental neurotoxicity. This research examines whether resveratrol (RV) influences the expression of genes associated with neurogenesis and survival after prenatal exposure to DZN.</p><p><strong>Materials and methods: </strong>Twenty-four pregnant Wistar rats were divided into DZN (40 mg/kg) group, RV (10 mg/kg), DZN+RV (40 mg/kg + 10 mg/kg), and Control. On day 21 of pregnancy, rats were cesarean sectioned, and the neonatal brains were examined using HPLC, GC-MS, real-time PCR, and histology techniques to assess RV's neuroprotective effects against DZN-induced toxicity. Data were statistically analyzed using SPSS and GraphPad Prism.</p><p><strong>Results: </strong>DZN significantly reduced neuronal survival and altered gene expression in fetal brains, with lower Ptf1α, SOX2, BDNF, and BCL2 levels and higher BAX levels compared to controls. The simultaneous administration of RV partially restored these molecular changes. Histological findings indicated that RV mitigated neuronal damage associated with DZN, resulting in decreased dark neuron formation and preserved myelin integrity in the hippocampus and thalamus.</p><p><strong>Conclusion: </strong>Collectively, these findings suggest that DZN exposure correlates with changes in the molecular and structural composition of the fetal brain, and that RV may help regulate these effects.</p>","PeriodicalId":14495,"journal":{"name":"Iranian Journal of Basic Medical Sciences","volume":"29 8","pages":"1249-1258"},"PeriodicalIF":3.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13527849/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148864665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Radiotherapy-associated changes in the miR-124/SP1 axis in a rat glioma model.","authors":"Elham Ghasemi, Mehdi Sadegh, Mahdieh Mondanizadeh, Behzad Khansarinejad, Fatemeh Seif","doi":"10.22038/ijbms.2026.95079.20506","DOIUrl":"10.22038/ijbms.2026.95079.20506","url":null,"abstract":"<p><strong>Objectives: </strong>Glioma is an aggressive brain tumor that frequently involves microRNA dysregulation. Rno-miR-124-3p is a brain-enriched miRNA involved in gene regulation, with Specificity Protein 1 (SP1) as its validated target. Radiotherapy remains a cornerstone of glioma treatment; however, its molecular effects on miRNA-transcription factor axes are not fully understood. We hypothesized that radiotherapy up-regulates Rno-miR-124-3p, thereby down-regulating SP1. Accordingly, this study evaluated their expression following radiotherapy in a rat glioma model.</p><p><strong>Materials and methods: </strong>C6 glioma cells were cultured, and an effective radiation dose was determined using an MTT assay. Sixty male Wistar rats were randomly assigned to six experimental groups: control, sham, glioma, and their corresponding radiotherapy-treated groups. Glioma was induced by stereotactic injection of C6 cells into the striatum. After radiotherapy, brain tissue was collected for histological and molecular analyses. Tumor expression of Rno-miR-124-3p and SP1 was quantified by real-time PCR.</p><p><strong>Results: </strong>In glioma-bearing rats, Rno-miR-124-3p expression was significantly down-regulated, whereas SP1 was significantly up-regulated compared with control groups (<i>P</i>≤0.05 and <i>P</i>≤0.01, respectively). After radiotherapy, an inverse pattern was observed, with increased Rno-miR-124-3p and decreased SP1 in glioma tissues (<i>P</i>≤0.05 and <i>P</i>≤0.01). Histological evaluation suggested structural differences between the glioma and radiotherapy-treated glioma groups.</p><p><strong>Conclusion: </strong>Glioma development alters the Rno-miR-124-3p/SP1 regulatory axis. Radiotherapy modulates this molecular pattern, suggesting a potential role for Rno-miR-124-3p in glioma-related pathways. Further studies are needed to clarify its functional and clinical relevance.</p>","PeriodicalId":14495,"journal":{"name":"Iranian Journal of Basic Medical Sciences","volume":"29 8","pages":"1211-1217"},"PeriodicalIF":3.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13527851/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148864725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Localized chemotherapy through nanofiber scaffolds: Progress, challenges, and future directions.","authors":"Akram Firouzi Amandi, Seyed Abbas Shahrtash, Samaneh Moradi, Mehdi Dadashpour, Tohid Kazemi","doi":"10.22038/ijbms.2026.92375.19939","DOIUrl":"10.22038/ijbms.2026.92375.19939","url":null,"abstract":"<p><p>Nanofiber-based localized chemotherapy has attracted growing attention as an effective approach to address the shortcomings of conventional systemic cancer therapies by enabling site-specific, sustained, and controlled drug delivery. This review focuses on the emerging role of implantable nanofibrous drug delivery systems in improving cancer treatment outcomes by reducing systemic side effects, enhancing therapeutic efficacy, and improving local tumor management. Special emphasis is placed on electrospinning, a widely used and flexible fabrication method for producing nanofibrous scaffolds, and on the development of chitosan-based nanocomposites and other functional polymer systems that offer high drug-loading capacity and adjustable release behavior. In addition, tumor-targeted drug delivery strategies, including surface modification and ligand-receptor-mediated targeting, are discussed for their potential to improve treatment selectivity and address multidrug resistance. Key challenges related to clinical translation, safety considerations, large-scale fabrication, and long-term performance are also critically analyzed. Finally, the review outlines future research directions aimed at advancing nanofiber-based localized chemotherapy toward practical clinical applications.</p>","PeriodicalId":14495,"journal":{"name":"Iranian Journal of Basic Medical Sciences","volume":"29 9","pages":"1296-1312"},"PeriodicalIF":3.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13546493/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148900531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined effects of EGCG and retinol on viability and apoptosis-related gene expression in Caco-2 and HCT-116 colon cancer cells.","authors":"Saeedeh Ghorbani, Jamshidkhan Chamani, Monireh Movahedi, Zahra Kianmehr","doi":"10.22038/ijbms.2026.92570.19990","DOIUrl":"https://doi.org/10.22038/ijbms.2026.92570.19990","url":null,"abstract":"<p><strong>Objectives: </strong>Colorectal cancer remains a leading cause of cancer-related deaths worldwide, emphasizing the need for novel therapeutic strategies. Epigallocatechin-3-gallate (EGCG), a major green tea polyphenol, and retinol, a vitamin A derivative with antioxidant properties, have shown anti-cancer activity individually. This study investigates the combined effects of EGCG and retinol on human colorectal cancer cell lines, Caco-2 and HCT-116.</p><p><strong>Materials and methods: </strong>Cytotoxic effects of EGCG and retinol, alone and in combination, were evaluated using resazurin-based cell viability assays. Intracellular reactive oxygen species (ROS) levels were measured by DCFDA assay to assess antioxidant activity. Changes in expression of apoptosis- and survival-related genes (p53, p21, AKT, and NF-κB) were analyzed using quantitative real-time PCR (RT-qPCR).</p><p><strong>Results: </strong>Combined treatment with EGCG and retinol reduced cell viability and intracellular ROS levels in both Caco-2 and HCT-116 cells. This treatment also modulated gene expression by increasing pro-apoptotic markers (p53 and p21) and decreasing anti-apoptotic markers (AKT and NF-κB), suggesting enhanced regulation of apoptosis-related pathways and inhibition of survival signaling.</p><p><strong>Conclusion: </strong>The combined effects of EGCG and retinol result in enhanced cytotoxicity, antioxidant activity, and modulation of apoptosis- and survival-related genes in colorectal cancer cells, supporting their potential as a combined therapeutic approach. Further studies are required to confirm these findings and explore clinical relevance.</p>","PeriodicalId":14495,"journal":{"name":"Iranian Journal of Basic Medical Sciences","volume":"29 6","pages":"909-914"},"PeriodicalIF":3.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13480766/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148793148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of microRNAs in major brain diseases, focusing on neuroinflammation and neuronal apoptosis.","authors":"Nimra Aziz, Ghulam Hussain, Qammar Abbas Abbas","doi":"10.22038/ijbms.2026.88184.19047","DOIUrl":"https://doi.org/10.22038/ijbms.2026.88184.19047","url":null,"abstract":"<p><p>MicroRNAs are non-coding small RNA molecules that play a significant role in regulating gene expression. Increasing lines of evidence have highlighted the microRNA dysregulation and neuroinflammation-associated apoptosis in common brain diseases, including Parkinson's disease, Alzheimer's disease, epilepsy, traumatic brain injury, depression, and migraine. In fact, microRNAs regulate multiple physiological and pathological processes, thus implicating them in both health and disease. Though studies have suggested that the alterations or modifications in microRNA-associated regulatory pathways might contribute to the disease pathogenesis, the underlying molecular mechanisms and the targeted genes remain exclusively unknown. We hope that the idea of using microRNAs as therapeutic targets for brain disorders is not far from reality, but important issues must be addressed before moving into clinical practice. The aim of this review is to enlighten the molecular mechanisms and targeted genes of microRNA implicated in the multifaceted brain disorders. Moreover, several microRNAs have been reported to be up-regulated following disease, but their targeted pathways have not been elucidated yet. This review also highlighted microRNAs that are expected to warrant further exploration of their mechanism of action. This comprehensive overview of the prediction of microRNAs' functions might be helpful in providing more efficient insight for the development of microRNA-based therapeutic interventions for neuropsychiatric and neurodegenerative diseases.</p>","PeriodicalId":14495,"journal":{"name":"Iranian Journal of Basic Medical Sciences","volume":"29 6","pages":"844-869"},"PeriodicalIF":3.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13480863/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148793215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hispidulin: A potential antihyperglycemic and anti-oxidant agent-mechanistic insights into its modulatory effects on PI3K, AKT, mTOR, IRS1, GSK-3β, and GLUT-4 pathways through in vitro and in vivo studies.","authors":"Muhammad Saad Tariq, Wafa Majeed, Bilal Aslam, Muhammad Shahid","doi":"10.22038/ijbms.2025.88589.19136","DOIUrl":"10.22038/ijbms.2025.88589.19136","url":null,"abstract":"<p><strong>Objectives: </strong>Hispidulin, a naturally occurring flavonoid with neuroprotective and anticancer properties, has shown therapeutic potential for the treatment of metabolic disorders, including diabetes mellitus. This study was designed to explore the anti-diabetic efficacy of hispidulin by examining its role in regulating glycemic markers, insulin responsiveness, oxidative stress, and the inflammatory response, and by evaluating transcriptional profiling of pivotal genes involved in the PI3K/AKT signaling cascade.</p><p><strong>Materials and methods: </strong>Experimental induction of type 2 diabetes was achieved using a high-fat diet regimen, followed by intraperitoneal administration of nicotinamide 110 mg/kg and streptozotocin 55 mg/kg. Following diabetes induction, rats were treated with hispidulin (10-20 mg/kg orally/day). Over 28 days, various parameters were assessed, including Fasting blood glucose, serum glucose, serum insulin, HOMA-IR, HOMA-β, QUICKI, CISI, oxidative stress markers (TAC, TOS, TBRAS, SOD, CAT, NO), and inflammatory cytokines (IL-6, TNF-α, CRP, NFkB). Gene expression levels of PI3K, AKT, mTOR, IRS-1, GSK-3β, and GLUT-4 were determined via qRT-PCR.</p><p><strong>Results: </strong>Hispidulin treatment significantly (<i>P</i><0.001) enhanced glycemic regulation and insulin sensitivity, as reflected by decreased fasting blood glucose levels and improved insulin indices. It favorably modulated oxidative stress markers and reduced the pro-inflammatory cytokines. Gene expression analysis indicated up-regulation of PI3K, AKT, mTOR, IRS-1, and GLUT-4, with down-regulation of GSK-3β, suggesting up-regulation of the PI3K/AKT signaling cascade.</p><p><strong>Conclusion: </strong>Hispidulin exhibits potent antidiabetic properties by improving insulin sensitivity, reducing oxidative stress and inflammation, and modulating key genes in the PI3K/AKT pathway. These findings suggest hispidulin as a therapeutic agent for managing type 2 diabetes mellitus.</p>","PeriodicalId":14495,"journal":{"name":"Iranian Journal of Basic Medical Sciences","volume":"29 2","pages":"240-248"},"PeriodicalIF":3.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13090975/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147722797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-inflammatory and anti-oxidant efficacy of origanum species and honey in a rat model of COPD.","authors":"Fadime Ovali, Hüsamettin Vatansev, Hakan Vatansev, Bayram Çolak, İsmail Harmankaya","doi":"10.22038/ijbms.2025.89695.19351","DOIUrl":"10.22038/ijbms.2025.89695.19351","url":null,"abstract":"<p><strong>Objectives: </strong>Chronic obstructive pulmonary disease (COPD) is a progressive airway condition characterized by chronic inflammation, oxidative stress, and tissue remodeling. Conventional treatments offer limited benefits in halting disease progression, prompting increased interest in natural agents with antioxidant and anti-inflammatory properties. This study aimed to evaluate the therapeutic effects of <i>Origanum onites</i> L<i>.</i>, <i>Origanum minutiflorum</i>, and honey in a rat model of elastase-induced COPD.</p><p><strong>Materials and methods: </strong>Thirty-eight female Wistar albino rats were divided into five groups: Control, COPD, <i>O. onites</i> L<i>.</i> treatment, <i>O. minutiflorum</i> treatment, and honey treatment. COPD was induced via intratracheal porcine pancreatic elastase. Treatments were administered orally for 15 days. Serum levels of IL-1α, IL-33, and HMGB1, and lung homogenate levels of MMP-9 and TIMP-1 were measured by ELISA. T cell subtypes (CD3⁺, CD4⁺, CD8⁺) were analyzed by flow cytometry. Blood gas parameters were evaluated, and histopathological scoring was performed.</p><p><strong>Results: </strong><i>Origanum onites</i> L<i>.</i>, <i>O. minutiflorum</i>, and honey exhibited modulatory effects on inflammatory and oxidative markers. O. minutiflorum significantly reduced IL-1α and IL-33 levels (<i>P<</i>0.05) and decreased MMP-9 expression. The honey group showed increased TIMP-1 levels and significantly improved blood oxygenation (pO₂ and sO₂). CD3⁺ and CD8⁺ T cell percentages were significantly higher in the treatment groups compared to the COPD group. Emphysema scores were significantly lower in the honey group (<i>P<</i>0.05), and lymphoid aggregates were observed only in the COPD group.</p><p><strong>Conclusion: </strong>The findings indicate that Origanum species and honey may have therapeutic potential for COPD by modulating inflammation, the immune response, and the protease-antiprotease balance. Further molecular and clinical studies are warranted.</p>","PeriodicalId":14495,"journal":{"name":"Iranian Journal of Basic Medical Sciences","volume":"29 2","pages":"231-239"},"PeriodicalIF":3.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13090945/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147722819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}