Iranian Journal of Basic Medical Sciences最新文献

{"title":"Synergistic effect of cold atmospheric plasma and methylene blue loaded nano micelles on treating human glioblastoma cells: An in vitro and molecular dynamics study.","authors":"Elahe Ahmadi, Armin Imanparast, Mehdi Hoseini, Shahrokh Naseri, Samaneh Soudmand Salarabadi, Ameneh Sazgarnia","doi":"10.22038/ijbms.2024.79858.17304","DOIUrl":"10.22038/ijbms.2024.79858.17304","url":null,"abstract":"<p><strong>Objectives: </strong>One of the most recent cancer treatment methods is cold atmospheric plasma (CAP), which destroys cancer cells without affecting healthy cells. Also, the created photons in the CAP flame can be used to excite a proper photosensitizing agent (PS). Therefore, using nano micelle systems containing a proper photosensitizer may be beneficial in raising the treatment efficacy of CAP. In this study, we utilized molecular dynamics (MD) simulation to optimize a nano micellar system containing methylene blue to take advantage of the induced photodynamic effect of a CAP generator with helium gas on a glioblastoma cell line.</p><p><strong>Materials and methods: </strong>Some micelle properties were first determined and optimized by MD with GROMACS software. Then, micelles containing methylene blue (Micelle-MB) and free methylene blue (MB) at various concentrations were prepared. Singlet oxygen dosimetry using 1,3-diphenylisobenzofuran (DPBF)was performed in the presence and absence of Micelle-MB and MB. Subsequently, the cytotoxicity of MB and Micelle-MB was evaluated on U87-MG cancer cells, and their half-maximal inhibitory concentrations (IC₅₀) were determined. After 48 hr of treatment, the percentage of cell survival was determined using the MTT test. The experiments were repeated at least three times. The synergy index was selected to compare the results.</p><p><strong>Results: </strong>Treatment with CAP and MB reduced the survival rate compared to the PS-free group with CAP. Results of singlet oxygen dosimetry showed that Micelle-MB might be more efficient in producing ROS. CAP treatment with Micelle-MB resulted in more cell death than free MB. In addition, cell viability decreased in Micelle-MB groups with increasing irradiation time in the three investigated irradiation times.</p><p><strong>Conclusion: </strong>Using Micelle-MB in the CAP treatment improves treatment efficiency in the U87-MG cell line.</p>","PeriodicalId":14495,"journal":{"name":"Iranian Journal of Basic Medical Sciences","volume":"28 3","pages":"299-309"},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11790199/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143189094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Echinacoside alleviates Ang II-induced cardiac fibrosis by enhancing the SIRT1/IL-11 pathway.","authors":"Yingbiao Wu, Zhongping Ning","doi":"10.22038/ijbms.2024.79837.17296","DOIUrl":"10.22038/ijbms.2024.79837.17296","url":null,"abstract":"<p><strong>Objectives: </strong>Echinacoside (ECH) is an anti-fibrotic phenylethanoid glycoside derived from the <i>Cistanche</i> plant that protects against cardiac dysfunction by mitigating apoptosis, oxidative stress, and fibrosis. Nevertheless, ECH's precise function and mechanisms in addressing cardiac fibrosis are still not fully understood.</p><p><strong>Materials and methods: </strong>In our current investigation, we induced cardiac fibrosis in mice by administering Angiotensin II (Ang II) and subsequently assessed the effects of ECH treatment four weeks post-fibrosis induction. Additionally, in an <i>in vitro</i> setting, we exposed cardiac fibroblasts (CFs) to Ang II to prove the anti-fibrotic mechanisms of ECH.</p><p><strong>Results: </strong>ECH treatment effectively reversed cardiac fibrosis in the mice model. ECH treatment significantly reduced the levels of fibrosis-related genes, such as α-SMA, Collagen I, and Collagen III (all, <i>P<</i>0.001). Moreover, it reduced the number of apoptotic cells and regulated the expression of apoptosis-related genes, such as BAX and BCL-2 (all, <i>P<</i>0.001). ECH treatment also positively affected serum levels of markers associated with cardiac fibrosis, including LDH, CK-MB, ANP, BNP, CTnl, and CTnT (all, <i>P<</i>0.001), in the <i>in vivo</i> experiments. In the <i>in vitro</i> studies, ECH pretreatment alleviated cardiac fibroblast apoptosis and reduced cell migration, collagen deposition, and MMP expression (all, <i>P<</i>0.001). In our <i>in vivo</i> and <i>in vitro</i> investigations, we observed that ECH treatment reversed the down-regulation of SIRT1 and up-regulation of IL-11 following cardiac fibrosis. The results suggest that the protective effects of ECH may involve regulating the SIRT1/IL-11 pathway.</p><p><strong>Conclusion: </strong>ECH may protect against Ang II-induced cardiac fibrosis via the SIRT1/IL-11 pathway.</p>","PeriodicalId":14495,"journal":{"name":"Iranian Journal of Basic Medical Sciences","volume":"28 1","pages":"130-139"},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11771342/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143058977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects and mechanism of puerarin on lactation of postpartum hypogalactia mice.","authors":"Ya-Jie Yang, Bing-Qing Liu, Yang Jiang, Man-Yu Zhou, Re-Yi-la Tuerxun, Hong-Shuang Liu, Yan Liao","doi":"10.22038/ijbms.2025.81350.17611","DOIUrl":"https://doi.org/10.22038/ijbms.2025.81350.17611","url":null,"abstract":"<p><strong>Objectives: </strong>Insufficient breast milk supply is a common reason cited for discontinuing breastfeeding prematurely. Natural galactagogues offer promise as a solution for mothers with low milk production. This study aimed to explore puerarin's potential effects and underlying mechanism on lactation of postpartum hypogalactia mice.</p><p><strong>Materials and methods: </strong>Postpartum mice were randomly assigned to five groups: control group, agalactosis model group, domperidone group (3.5 mg/kg), low dose puerarin group (18 mg/kg), and high dose puerarin group (72 mg/kg). The effects of puerarin on postpartum hypogalactia mice were evaluated by lactation indicators and pathological morphology. Related hormones and prolactin receptor (PRLR)/Janus kinase 2 (JAK2)/signal transduction and activator (STAT) 5 signaling pathway were also measured.</p><p><strong>Results: </strong>Puerarin significantly improved lactation yield and stimulated mammary gland development in postpartum hypogalactia mice. Additionally, puerarin increased the expression levels of β-casein, fatty acid synthase (FAS), and glucose transporter 1 (GLUT1). Mechanically, puerarin stimulated secretion of prolactin (PRL), estradiol (E2), and progesterone (P4) in agalactosis mice. Puerarin also substantially increased PRLR, JAK2, and STAT5a expression levels in postpartum hypogalactia mice.</p><p><strong>Conclusion: </strong>This study suggested that puerarin may promote lactation by stimulating PRL secretion and activating the PRLR/JAK2/STAT5 signaling pathway.</p>","PeriodicalId":14495,"journal":{"name":"Iranian Journal of Basic Medical Sciences","volume":"28 6","pages":"739-745"},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12057751/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144017558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating therapeutic efficacy of dacarbazine and temozolomide, alone and in combination with <i>BRAF</i> ^(V600E) siRNA in A375 human melanoma cell line.","authors":"Fatemeh Tabandeh, Rana Moradian Tehrani, Mohammadreza Sharifi, Elmira Toopchi","doi":"10.22038/ijbms.2025.84187.18208","DOIUrl":"https://doi.org/10.22038/ijbms.2025.84187.18208","url":null,"abstract":"<p><strong>Objectives: </strong>Melanoma is one of the most aggressive and deadly skin cancers. Despite advances, effective melanoma treatment is challenging, often requiring a shift from individual therapies to combination approaches. This study explores whether combining dacarbazine (DTIC) and temozolomide (TMZ) with the siRNA approach holds promise for melanoma treatment.</p><p><strong>Materials and methods: </strong>To determine the IC₅₀ values of DTIC and TMZ, the A375 cell line was treated with different drug concentrations for 24-72 hr. The best exposure time of BRAF siRNA transfection was performed. Subsequently, cell viability (using the MTT assay), apoptosis (by flow cytometry), and gene expression levels of B-Raf proto-oncogene, serine/threonine kinase (<i>BRAF</i>), caspase 3 (<i>CASP3</i>), and phosphoinositide-3-kinase regulatory subunit 3 (<i>PIK3R3</i>) genes (by quantitative real-time PCR) were assessed in the treated groups (i.e., control, negative controls, DTIC alone, TMZalone, DTIC+ TMZ, BRAF(V600E)siRNA alone, siRNA+ DTIC, siRNA+ TMZ, and siRNA+ DTIC+ TMZ groups).</p><p><strong>Results: </strong>Cell viability significantly decreased in the chemotherapy-only and siRNA+drug groups, although no difference was observed between them. The apoptosis percentage in all treated groups indicated a significant difference compared to the control group. The expression of the <i>BRAF</i> gene notably decreased in the BRAF (<i>V600E</i>) siRNA +drug groups compared to the chemotherapy groups. Despite overexpression of <i>CASP3</i> in the chemotherapy-treated groups, the most effective enhancement was noted in the siRNA+DTIC+TMZ group (<i>P</i><0.0001). The mean expression of the <i>PIK3R3</i> gene in siRNA+chemotherapy groups revealed a notable reduction.</p><p><strong>Conclusion: </strong>These findings suggest that the siRNA-transfected treatment groups have the potential to provide therapeutic effects comparable to those of chemotherapy.</p>","PeriodicalId":14495,"journal":{"name":"Iranian Journal of Basic Medical Sciences","volume":"28 6","pages":"772-783"},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12057749/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144003271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PGC-1α agonist ZLN005 ameliorates OVA-induced asthma in BALB/c mice through modulating the NF-κB-p65/NLRP3 pathway.","authors":"Rui Fang, Yan Cheng, Ping Chen, Jing Hu, Liqi Yang","doi":"10.22038/ijbms.2025.83166.17982","DOIUrl":"https://doi.org/10.22038/ijbms.2025.83166.17982","url":null,"abstract":"<p><strong>Objectives: </strong>Asthma is a complex inflammatory disease of the lungs marked by increased infiltration of leukocytes into the airways, which restricts respiratory function. Proliferator-activated receptor-γ coactivator-1 alpha (PGC-1α) has been recognized as an essential immunomodulator and has the potential as a novel anti-inflammatory target in asthma. The current study aims to investigate the functions of PGC-1α in ovalbumin (OVA)-sensitized asthmatic mice and underlying mechanisms.</p><p><strong>Materials and methods: </strong>BALB/c mouse asthma model was induced by OVA <i>in vivo</i>. The therapeutic effects of PGC-1α agonist (ZLN005) on asthma were assessed by histological and biochemical analysis. In addition, we integrated real-time qPCR, western blotting, and immunofluorescence analysis to reveal the underlying mechanism.</p><p><strong>Results: </strong>In the lung tissue of asthmatic mice, PGC-1α levels were down-regulated. Diff-Quik staining indicated that ZLN005 therapy on asthmatic mice reduced the number of inflammatory cells (eosinophilic granulocytes, neutrophils, lymphocytes, and mononuclear macrophages) in bronchoalveolar lavage fluid (BALF), ameliorated pathologic alterations in lung tissues. ZLN005 alleviated airway structure and inflammation, as well as down-regulating the serum immunoglobulin E (IgE), OVA-specific IgE, and T-helper 2 (Th2) cytokines (interleukin (IL)-4, IL-5, and IL-13) expression. Mechanistically, the results showed that ZLN005, through the NF-κB-p65 axis, prominently inhibited the activation of the NLRP3 inflammasome and reduced the levels of the NLRP3 downstream targets IL-1β and IL-18.</p><p><strong>Conclusion: </strong>PGC-1α agonist (ZLN005) regulated lung inflammation in asthmatic mice by inhibiting the NF-κB-p65/NLRP3 signaling pathway, supporting that ZLN005 may be a candidate for future asthma treatment.</p>","PeriodicalId":14495,"journal":{"name":"Iranian Journal of Basic Medical Sciences","volume":"28 6","pages":"710-717"},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12057745/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144003275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ethanol extract of <i>Garcinia kola</i> seeds alleviates HGHFD/STZ-induced nonalcoholic fatty liver disease in diabetic rats by modulating oxidative stress, inflammation, and lipid accumulation.","authors":"Xiaojing Sun, Yanxiang Yuan, Xianhao Xin, Ping Sun, Yunqi Sun, Mi Xie, Yuefei Wang, Shan Huang, Bin Li","doi":"10.22038/ijbms.2025.82786.17889","DOIUrl":"10.22038/ijbms.2025.82786.17889","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the ameliorative effects of <i>Garcinia kola</i> ethanol extract (EGK) on type 2 diabetes mellitus (T2DM) combined with nonalcoholic fatty liver disease (NAFLD) and to explore its underlying mechanisms.</p><p><strong>Materials and methods: </strong><i>In vivo</i>, a T2DM rat model was established using HGHFD/STZ. <i>In vitro</i>, HepG2 cells were induced with FFA to create a model of lipid accumulation. Lipid accumulation (LA), oxidative stress (OS) levels, and inflammatory markers were measured using kit methods. Additionally, the expression of the SREBP-1c pathway was detected by immunohistochemistry and western blot (WB) to further understand the potential mechanism of EGK's protective effect on diabetic liver injury.</p><p><strong>Results: </strong><i>In vivo</i>, EGK significantly reduced blood glucose levels (<i>P</i><0.01), restored body weight (<i>P</i><0.01), and improved liver LA, OS, and inflammatory levels (<i>P</i><0.01) in diabetic rats. Histopathological results indicated that EGK effectively ameliorated diabetes-induced liver injury. Immunohistochemistry and WB results revealed that EGK significantly down-regulated the expression of the SREBP-1c pathway (<i>P</i><0.01). <i>In vitro</i>, EGK markedly improved lipid accumulation, oxidative stress, and inflammation levels in HepG2 cells (<i>P</i><0.01). Immunofluorescence and WB results showed that EGK significantly reduced the expression of the SREBP-1c pathway (<i>P</i><0.01).</p><p><strong>Conclusion: </strong>EGK alleviates T2DM combined with NAFLD by reducing lipid accumulation through the inhibition of oxidative stress, inflammatory responses, and the SREBP-1c signaling pathway.</p>","PeriodicalId":14495,"journal":{"name":"Iranian Journal of Basic Medical Sciences","volume":"28 5","pages":"662-670"},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12258791/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144642471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Facile fabrication of selegiline-loaded alginate hydrogel for neuroprotection and functional recovery in a rat model of spinal cord injury through localized spinal delivery.","authors":"Ramin Abrishami, Ramtin Farhadi, Mehri Farhang Ranjbar, Seyed Hadi Aghili, Maryam Baeeri","doi":"10.22038/ijbms.2025.81837.17706","DOIUrl":"10.22038/ijbms.2025.81837.17706","url":null,"abstract":"<p><strong>Objectives: </strong>Spinal cord injury (SCI) is a highly disabling and fatal disorder with no effective treatment to date. Selegiline, a selective MAO-B inhibitor, has shown new neuroprotective and neurorescuing effects with various beneficial effects on neuron-associated disorders. These effects have triggered investigations into its impact on different neuron-associated disorders and SCI. Thus, in continuation of the previous studies, this study evaluates the local therapeutic effects of selegiline-loaded alginate hydrogel on SCI by analyzing apoptotic factors, histological factors, and improvements in locomotor function and neuropathic pain.</p><p><strong>Materials and methods: </strong>Hydrogels were fabricated via cross-linking gelation method and characterized by FT-IR and SEM analysis. Selegiline release from hydrogels was evaluated by UV spectroscopy, and hydrogel biocompatibilities were verified through an MTT assay. Afterward, 36 rats were divided into six groups: sham, negative group, treated with empty hydrogel, and three selegiline-treated groups (2.5, 5, and 10 mg/kg). After 28 days, the locomotor activity, the expression of Bax and Bcl2 (apoptosis index), and GFAP changes in the lesion site were assessed using Basso, Beattie, and Bresnahan (BBB) scale, western blot technique, and immunohistochemical assay, respectively.</p><p><strong>Results: </strong>Hydrogel tests showed the suitability of hydrogels and sustained selegiline release from them. Rats treated with selegiline-loaded hydrogels showed significant locomotor improvement and reduced apoptosis indices in SCI-induced rats (<i>P</i>≤0.05). Additionally, GFAP immunohistochemistry analysis indicated notable histological improvements.</p><p><strong>Conclusion: </strong>Findings suggest that selegiline-loaded hydrogels can improve SCI through apoptosis inhibition and neurorescuing effects. Further clinical studies are warranted to validate these findings in human SCI.</p>","PeriodicalId":14495,"journal":{"name":"Iranian Journal of Basic Medical Sciences","volume":"28 5","pages":"627-637"},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12258782/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144642472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Meloxicam mitigated methylglyoxal-induced glycative stress in rats.","authors":"Talha Bin Fayyaz, Ghulam Abbas, Hammad Ahmed, Najeeb Khatian, Shumaila Usman, Uzair Nisar, Noor Ul Ain, Yamna Khurshid, Syed Abid Ali","doi":"10.22038/ijbms.2025.83856.18143","DOIUrl":"10.22038/ijbms.2025.83856.18143","url":null,"abstract":"<p><strong>Objectives: </strong>Glycation is one of the primary underlying processes attributed to senescence and related diseases. No medicine currently targets this harmful manifestation. Drug repurposing is an efficient and cost-effective way of developing drugs. The present study evaluated meloxicam, a clinically used NSAID, for its ability to offer protection against glycative stress.</p><p><strong>Materials and methods: </strong>Methylglyoxal (MGO; 17.25 mg/kg) was administered for two weeks to create a rat model of glycative stress. Aminoguanidine (AG; 50 mg/kg) and Meloxicam (MEL; 0.15, 0.3, and 0.6 mg/kg) were used as standard and test agents, respectively. Afterward, the cognitive (Morris Water Maze), liver (LFT), and kidney (Creatinine) functioning were evaluated. The expression of genes of interest (TNF-α, RAGE, BACE, Glyoxalase, and VEGF) were estimated (qPCR) in the liver, brain, and kidney along with histopathology (H&E staining). Carboxymethyllysine (CML) levels in rat plasma were evaluated via ELISA.</p><p><strong>Results: </strong>MEL treatment has significantly (<i>P</i><0.05) protected the MGO-induced cognitive (duration in target quadrant, time taken to get to target quadrant, and the frequency of crossings via platform location), hepatic, and renal impairment. The qPCR data revealed that MEL prevented MGO-induced enhancement in the expression of genes of interest. Additionally, the CML levels were significantly (<i>P</i><0.005) normalized by concomitant administration of MEL. Histopathological examination did not reveal any remarkable outcomes.</p><p><strong>Conclusion: </strong>MEL has significantly mitigated the rats' MGO-induced cognitive, liver, and kidney impairments. Hence, it appears to be a potential molecule for repurposing as an antiglycation agent.</p>","PeriodicalId":14495,"journal":{"name":"Iranian Journal of Basic Medical Sciences","volume":"28 5","pages":"647-654"},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12258788/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144642475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Testicular extracellular matrix/gelatin-based scaffold using gas foaming to support spermatogonial stem cells.","authors":"Maryam Momeni, Mohammad Kazemi Ashtiani, Zahra Bashiri, Zohreh Bagher, Hamidreza Asgari, Morteza Koruji","doi":"10.22038/ijbms.2025.83885.18150","DOIUrl":"10.22038/ijbms.2025.83885.18150","url":null,"abstract":"<p><strong>Objectives: </strong>Developing a bioactive testicular scaffold has been proposed as a potential option to preserve male fertility. Using the gas foaming method, this study aimed to fabricate an effective and highly porous scaffold derived from a gelatin-testicular extracellular matrix.</p><p><strong>Materials and methods: </strong>Male ram testis decellularization was performed using a combination of NaCl buffer and Triton. Then, evaluations were done for 4',6-diamidino-2-phenylindole, hematoxylin-eosin staining, and quantitative DNA content. Masson's trichrome, Alcian blue, and orcein staining were conducted to ensure the maintenance of ECM components post-decellularization. Porous scaffolds were fabricated using gelatin incorporation with various concentrations of extracted ECM via the gas foaming method. The mechanical and structural characteristics of the scaffolds, along with evaluations of cell spreading and penetration depth, were performed. Furthermore, scaffolds were used to culture mouse spermatogonial stem cells to investigate the morphology, viability, and adhesion of the cells on the scaffolds.</p><p><strong>Results: </strong>Our results showed successful decellularization of testicular tissue, resulting in significant removal of DNA content while preserving ECM major components. The hybrid scaffolds exhibited uniform porous microstructures with a pore size average ranging from 298-330 μm. There were no significant differences in biodegradation and swelling ratios between the scaffolds. The cell penetration index significantly increased in gel-ECM by 5% compared to other groups. Also, ECM5% led to increased cell attachment and viability, proper compressive strength, and a decrease in Young's module.</p><p><strong>Conclusion: </strong>Our study suggests that using a combination of testicular ECM and gelatin shows promise in constructing bioartificial testes through the gas-foaming method.</p>","PeriodicalId":14495,"journal":{"name":"Iranian Journal of Basic Medical Sciences","volume":"28 8","pages":"1107-1118"},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12203833/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144527982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic potential of casticin in alleviating ovarian torsion/detorsion-induced ovarian and lung injuries.","authors":"Ersen Eraslan, Ayhan Tanyeli̇, Mustafa Can Güler, Fazile Nur Eki̇nci̇ Akdemi̇r, Ömer Topdaği, Şahin Yazici, Derya Güzel Erdoğan, Esra Çinar Tanriverdi̇, Selim Çomakli","doi":"10.22038/ijbms.2025.85990.18572","DOIUrl":"10.22038/ijbms.2025.85990.18572","url":null,"abstract":"<p><strong>Objectives: </strong>Ovarian torsion is a critical gynecological emergency caused by the twisting of the ovary around its supporting structures, resulting in compromised blood flow and potential ovarian damage. Ovarian torsion/detorsion (T/D) can lead to severe complications, including infertility, if not promptly addressed. This study aimed to investigate the therapeutic effects of casticin (CAST) on ovarian and lung tissue injuries induced by a bilateral ovarian T/D model in rats.</p><p><strong>Materials and methods: </strong>Experimental animals were randomly allocated into groups of sham, T/D, CAST 5 mg/kg, and CAST 10 mg/kg. Ovarian and lung tissues were subjected to biochemical, histopathological, and immunohistochemical analyses.</p><p><strong>Results: </strong>In the T/D group, markers of oxidative stress and inflammation, including myeloperoxidase (MPO) activity, malondialdehyde (MDA), oxidative stress index (OSI), tumor necrosis factor-alpha (TNF-α), and 8-hydroxy-2' deoxyguanosine (8-OHdG), were significantly elevated (<i>P<</i>0.05). Conversely, superoxide dismutase (SOD) activity and total antioxidant status (TAS) levels were notably decreased (<i>P<</i>0.05). CAST administration significantly attenuated tissue damage by reducing oxidative stress and inflammatory markers while enhancing antioxidant defense (<i>P<</i>0.05).</p><p><strong>Conclusion: </strong>CAST demonstrated a therapeutic effect against ovarian and lung tissue damage induced by T/D, suggesting that it may serve as a potential therapeutic agent for treating ovarian T/D-related injuries. These findings underscore the potential of CAST in clinical settings, particularly as a novel intervention to mitigate complications associated with I/R injuries in gynecological emergencies.</p>","PeriodicalId":14495,"journal":{"name":"Iranian Journal of Basic Medical Sciences","volume":"28 8","pages":"1057-1064"},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12203820/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144527983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}