Iranian Journal of Basic Medical Sciences最新文献_第6页

Quality by design approach for development and characterization of gabapentin-loaded solid lipid nanoparticles for intranasal delivery: In vitro, ex vivo, and histopathological evaluation. 用于鼻内给药的加巴喷丁固体脂质纳米颗粒的开发和表征的质量设计方法：体外、体内和组织病理学评估。

IF 2.2 4区医学

Iranian Journal of Basic Medical Sciences Pub Date : 2024-01-01 DOI: 10.22038/IJBMS.2024.76281.16511

Mahmut Ozan Toksoy, Fırat Aşır, Mert Can Güzel

{"title":"Quality by design approach for development and characterization of gabapentin-loaded solid lipid nanoparticles for intranasal delivery: In vitro, ex vivo, and histopathological evaluation.","authors":"Mahmut Ozan Toksoy, Fırat Aşır, Mert Can Güzel","doi":"10.22038/IJBMS.2024.76281.16511","DOIUrl":"10.22038/IJBMS.2024.76281.16511","url":null,"abstract":"Objectives: \"Quality by Design\" (QbD) is a novel approach to product development that involves understanding the product and process, as well as the relationship between critical quality attributes (CQA) and critical process parameters (CPP). This study aimed to optimize the gabapentin-loaded solid lipid nanoparticle formulation (GP-SLN) using a QbD approach and evaluate in vitro and ex vivo performance.Materials and methods: The GP-SLN formulation was created using the microemulsion method by combining Gelucire 48/16, Tween 80, and Plurol Oleique CC 497. The Box-Behnken experimental design was adopted to investigate the effects of independent factors on dependent factors. The GP-SLN formulation was assessed based on particle size and distribution, zeta potential, morphology, entrapment efficiency, release kinetics, permeation parameters, stability, and nasal toxicity.Results: The nanoparticles had a cubical shape with a particle size of 185.3±45.6 nm, a zeta potential of -24±3.53 mV, and an entrapment efficiency of 82.57±4.02%. The particle size and zeta potential of the GP-SLNs remained consistent for 3 months and followed Weibull kinetics with a significantly higher ex vivo permeability (1.7 fold) than a gabapentin solution (GP-SOL). Histopathology studies showed that intranasal administration of the GP-SLN formulation had no harmful effects.Conclusion: The current study reports the successful development of a GP-SLN formulation using QbD. A sustained release of GP was achieved and its nasal permeability was increased. Solid lipid nanoparticles with optimum particle size and high entrapment efficiency may offer a promising approach for the intranasal delivery of drugs.","PeriodicalId":14495,"journal":{"name":"Iranian Journal of Basic Medical Sciences","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11127077/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141154257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Crocin nano-chitosan-coated compound improves anxiety disorders, learning, and spatial memory in Alzheimer's model induced by beta-amyloid in rats. 谷氨酰胺纳米壳聚糖涂层化合物可改善β-淀粉样蛋白诱导的阿尔茨海默氏症模型大鼠的焦虑症、学习和空间记忆。

IF 2.2 4区医学

Iranian Journal of Basic Medical Sciences Pub Date : 2024-01-01 DOI: 10.22038/IJBMS.2024.74823.16247

Gholam Hossein Meftahi, Mohsen Khodadadi, Gila Pirzad Jahromi, Masoud Ezami Razliqi, Habib Valipour

{"title":"Crocin nano-chitosan-coated compound improves anxiety disorders, learning, and spatial memory in Alzheimer's model induced by beta-amyloid in rats.","authors":"Gholam Hossein Meftahi, Mohsen Khodadadi, Gila Pirzad Jahromi, Masoud Ezami Razliqi, Habib Valipour","doi":"10.22038/IJBMS.2024.74823.16247","DOIUrl":"10.22038/IJBMS.2024.74823.16247","url":null,"abstract":"Objectives: Alzheimer's disease (AD) is a neurodegenerative disease that results in the gradual breakdown of brain tissue, causing the deterioration of intellectual function and ability. Crocin is a saffron carotenoid compound proven to have excellent neuroprotective and anti-inflammation properties, although it has some limitations such as low stability and bioavailability. Therefore, in the current research, we tried to improve these limitations by using nanotechnology and chitosan as the carrier. Our study examined the therapeutic effects of crocin nano-chitosan-coated compound and compared it with intact crocin in lower dosages than other studies in AD rat models.Materials and methods: Encapsulating crocin into chitosan nanoparticles was done through a modified technique to improve its limitations. The AD rat model was induced by bilaterally injecting beta-amyloid (Aβ) peptide into the frontal lobe using a stereotaxic device. To evaluate memory, we conducted the Barnes maze test, and to evaluate anxiety, we used the elevated plus maze test. Also, histological tests were conducted to evaluate neuronal damage in each group.Results: Crocin nano-chitosan-coated administration significantly improved specific memory indicators compared to the Aβ and other treated groups. A significant decrease in anxiety indicators was detected compared to the Aβ and other treated groups. Finally, the results of hippocampus staining indicated a meaningful difference between the Aβ group and other treated groups, compared to the crocin nano-chitosan-coated group.Conclusion: Treatment with low dosages of crocin in the nano-coated form exhibited great efficacy in reducing AD's adverse effects compared to the same dosage of intact crocin.","PeriodicalId":14495,"journal":{"name":"Iranian Journal of Basic Medical Sciences","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11127085/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141155301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential antiaging activity of secretome gel of human Wharton's jelly mesenchymal stem cells (hWJ-MSCs) in UV-induced mice models. 人沃顿果冻间充质干细胞（hWJ-MSCs）分泌物凝胶在紫外线诱导的小鼠模型中的潜在抗衰老活性。

IF 2.2 4区医学

Iranian Journal of Basic Medical Sciences Pub Date : 2024-01-01 DOI: 10.22038/IJBMS.2024.70825.15385

Wahyu Widowati, Ahmad Faried, Achmad Adam, Deni Rahmat, Hanna Sari Widya Kusuma, Nindia Salsabila Mia Dewi, Marisca Evalina Gondokesumo, Rizal Rizal, Ita Margaretha Nainggolan, Massoud Vosough

{"title":"Potential antiaging activity of secretome gel of human Wharton's jelly mesenchymal stem cells (hWJ-MSCs) in UV-induced mice models.","authors":"Wahyu Widowati, Ahmad Faried, Achmad Adam, Deni Rahmat, Hanna Sari Widya Kusuma, Nindia Salsabila Mia Dewi, Marisca Evalina Gondokesumo, Rizal Rizal, Ita Margaretha Nainggolan, Massoud Vosough","doi":"10.22038/IJBMS.2024.70825.15385","DOIUrl":"10.22038/IJBMS.2024.70825.15385","url":null,"abstract":"Objectives: Skin aging is a degenerative process that can be induced by UV irradiation. UV radiation can produce reactive oxidate stress which causes premature aging. This study aims to examine the antiaging potential of secretome gel (SC) from human Wharton Jelly Mesenchymal Stem Cells (hWJ-MSCs) in a UVB-induced mice model.Materials and methods: The secretome was obtained from hWJ-MSCs and made in gel form. Male mice were radiated by UVB for 15 min twice daily for 14 days. The gel was topically applied to the mice's dorsal skin. Two treatments of secretome gel: secretome 1 is applied once and secretome 2 is applied twice daily after UVB radiation. TGF-β1, IL-10, and IL-18 gene expression was determined using RT-PCR. Hematoxylin Eosin staining was used to observe the inflammation and collagen density of skin tissue. An immunohistochemistry assay was used to analyze the protein expression of P53, COL4A1, MMP-2, and MMP-13. The data were statistically analyzed using the ANOVA test followed by the Tukey post hoc test (P<0.05).Results: UVB induction caused loss of collagen, increasing inflammation and high expression of aging mediators. SC increased the gene expression of TGF-β1 and IL-10 and decreased IL-18 gene expression. Histopathological tests showed that SG increased collagen density, lowered inflammation, and repaired cell damage in skin tissue. Immunohistochemistry test showed that SC decreased MMP-2, MMP-13, and P53 expression, in contrast, increased COL4A1.Conclusion: The secretome gel of hWJ-MSCs showed antiaging activities with potential for preventing and curing skin aging.","PeriodicalId":14495,"journal":{"name":"Iranian Journal of Basic Medical Sciences","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141154253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lactosylated lipid calcium phosphate-based nanoparticles: A promising approach for efficient DNA delivery to hepatocytes. 乳糖基脂质磷酸钙基纳米颗粒：向肝细胞高效递送 DNA 的有效方法。

IF 2.1 4区医学

Iranian Journal of Basic Medical Sciences Pub Date : 2024-01-01 DOI: 10.22038/IJBMS.2024.76683.16602

Masoomeh Khalifeh, Ali Badiee, Navid Ramezanian, Amirhossein Sahebkar, Atena Farahpour, Reza Kazemi Oskuee

{"title":"Lactosylated lipid calcium phosphate-based nanoparticles: A promising approach for efficient DNA delivery to hepatocytes.","authors":"Masoomeh Khalifeh, Ali Badiee, Navid Ramezanian, Amirhossein Sahebkar, Atena Farahpour, Reza Kazemi Oskuee","doi":"10.22038/IJBMS.2024.76683.16602","DOIUrl":"10.22038/IJBMS.2024.76683.16602","url":null,"abstract":"Objectives: For safe and effective gene therapy, the ability to deliver the therapeutic nucleic acid to the target sites is crucial. In this study, lactosylated lipid phosphate calcium nanoparticles (lac-LCP) were developed for targeted delivery of pDNA to the hepatocyte cells. The lac-LCP formulation contained lactose-modified cholesterol (CHL), a ligand that binds to the asialoglycoprotein receptor (ASGR) expressed on hepatocytes, and polyethyleneimine (PEI) in the core.Materials and methods: Fourier transform infrared spectroscopy (FT-IR) and nuclear magnetic resonance (NMR) were used to monitor the chemical modification, and the physicochemical properties of NPs were studied using dynamic light scattering (DLS) and transmission electron microscopy (TEM). To evaluate transfection efficiency, cellular uptake and GFP expression were assessed using fluorescence microscopy and flow cytometry.Results: The results revealed that lactose-targeted particles (lac-LCP) had a significant increase in cellular uptake by hepatocytes. The inclusion of a low molecular weight PEI (1.8 KDa) with a low PEI/pDNA ratio of 1 in the core of LCP, elicited high degrees of GFP protein expression (by 5 and 6-fold), which exhibited significantly higher efficiency than PEI 1.8 KDa and Lipofectamine.Conclusion: The successful functionalization and nuclear delivery of LCP NPs described here indicate its promise as an efficient delivery vector to hepatocyte nuclei.","PeriodicalId":14495,"journal":{"name":"Iranian Journal of Basic Medical Sciences","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11193503/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141442671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective role of alpha-lipoic acid against rhabdomyolysis-induced acute kidney injury in rats. 硫辛酸对横纹肌溶解引起的大鼠急性肾损伤的保护作用

IF 2.1 4区医学

Iranian Journal of Basic Medical Sciences Pub Date : 2024-01-01 DOI: 10.22038/IJBMS.2024.74864.16252

Sadaf Nouripour, Soghra Mehri, Tahereh Aminifard, Arezoo Hosseini, Abolfazl Khajavi Rad, Amirhossein Jafarian, Hossein Hosseinzadeh

{"title":"Protective role of alpha-lipoic acid against rhabdomyolysis-induced acute kidney injury in rats.","authors":"Sadaf Nouripour, Soghra Mehri, Tahereh Aminifard, Arezoo Hosseini, Abolfazl Khajavi Rad, Amirhossein Jafarian, Hossein Hosseinzadeh","doi":"10.22038/IJBMS.2024.74864.16252","DOIUrl":"10.22038/IJBMS.2024.74864.16252","url":null,"abstract":"Objectives: Rhabdomyolysis, a potentially life-threatening condition, occurs when myoglobin is released from damaged muscle cells, leading to acute kidney injury (AKI). Alpha lipoic acid (ALA), an organosulfur compound known for its anti-oxidant and anti-inflammatory properties, was examined in this study for its potential impact on rhabdomyolysis-induced AKI in rats.Materials and methods: Six groups of rats were included in the study, with each group consisting of six rats (n=6): Control, rhabdomyolysis, rhabdomyolysis treated with different doses of ALA (5, 10, and 20 mg/kg), and ALA alone (20 mg/kg) groups. Rhabdomyolysis was induced by intramuscular injection of glycerol on the first day of the experiment, while ALA was administered intraperitoneally for four consecutive days. Renal function parameters, oxidative stress markers, and histological changes in the kidneys were evaluated. Western blot analysis was performed to measure the levels of neutrophil gelatinase-associated lipocalin (NGAL) and tumor necrosis factor-alpha (TNF-α) proteins.Results: A significant increase in serum urea, creatinine, renal malondialdehyde, NGAl, and TNF-α protein levels was observed in glycerol-injected rats. In addition, a significant decrease in glutathione was recorded. Compared to the rhabdomyolysis group, treatment with ALA recovered kidney histological and biochemical abnormalities.Conclusion: Results suggest that rhabdomyolysis-induced AKI is associated with increased oxidative stress and inflammation. Treatment with ALA improved kidney histological abnormalities and reduced oxidative stress markers in rats. Therefore, ALA may have a potential protective effect against rhabdomyolysis-induced AKI.","PeriodicalId":14495,"journal":{"name":"Iranian Journal of Basic Medical Sciences","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11193498/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141442675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stimulating effect of nanocurcumin and crocin on proliferation and pluripotency of bone marrow-derived mesenchymal stem cells. 纳米姜黄素和巴豆素对骨髓间充质干细胞增殖和多能性的促进作用

IF 2.1 4区医学

Iranian Journal of Basic Medical Sciences Pub Date : 2024-01-01 DOI: 10.22038/IJBMS.2024.74397.16197

Nasim Sabouni, Mojgan Mohammadi, Amir Reza Boroumand, Sepideh Palizban, Jalil Tavakol Afshari

{"title":"Stimulating effect of nanocurcumin and crocin on proliferation and pluripotency of bone marrow-derived mesenchymal stem cells.","authors":"Nasim Sabouni, Mojgan Mohammadi, Amir Reza Boroumand, Sepideh Palizban, Jalil Tavakol Afshari","doi":"10.22038/IJBMS.2024.74397.16197","DOIUrl":"10.22038/IJBMS.2024.74397.16197","url":null,"abstract":"Objectives: Enhancement of proliferation, pluripotency, and self-renewal capacity as the unique features of MSCs can improve their therapeutic potential to regenerate tissues. In this context, crocin and curcumin, carotenoid compounds with outstanding medicinal properties, could be promising for cell protection and growth. This study aimed to evaluate the impact of nanocurcumin and crocin on BM-MSCs proliferation and pluripotency in vitro.Materials and methods: BM-MSC were isolated from the iliac crest of SCI patients who were candidates for stem cell therapy. The effect of crocin and nanocurcumin on MSC proliferation was evaluated using MTT and PDT assay. The percentage of apoptotic MSCs was measured by flow cytometry. Furthermore, mRNA and protein expression of OCT4 and SOX2 as the proliferation and self-renewal related genes were quantified by real-time PCR and western blotting, respectively.Results: Our findings demonstrated that only low concentrations of nanocurcumin (0.3 and 0.7 µM) and crocin (2.5 5 µM) significantly affected MSCs proliferation and protected them from apoptosis. Also, crocin and nanocurcumin at low doses caused an elevation in the mRNA and protein expression levels of OCT4 and SOX2 genes. In contrast, high concentrations decreased the survival of MSCs and led to increased apoptosis compared with the untreated group.Conclusion: Our results suggest that using nanocurcumin and crocin separately in culturing MSCs can be considered proliferative agents to prepare the more advantageous tool for cell therapies. However, more in vitro and preclinical research is needed in this area.","PeriodicalId":14495,"journal":{"name":"Iranian Journal of Basic Medical Sciences","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11266738/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141758772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesized diterpene lactone derivative attenuated Freund's complete adjuvant-induced arthritis in Wistar rats. 合成的二萜内酯衍生物可减轻弗氏完全佐剂诱导的 Wistar 大鼠关节炎。

IF 2.1 4区医学

Iranian Journal of Basic Medical Sciences Pub Date : 2024-01-01 DOI: 10.22038/IJBMS.2024.75023.16295

Patrick Francis Kimariyo, Sony Priya Kurati, Perupogu Suvarna Babu, Alfredi Alfred Moyo, Murali Krishna Kumar Muthyala

{"title":"Synthesized diterpene lactone derivative attenuated Freund's complete adjuvant-induced arthritis in Wistar rats.","authors":"Patrick Francis Kimariyo, Sony Priya Kurati, Perupogu Suvarna Babu, Alfredi Alfred Moyo, Murali Krishna Kumar Muthyala","doi":"10.22038/IJBMS.2024.75023.16295","DOIUrl":"10.22038/IJBMS.2024.75023.16295","url":null,"abstract":"Objectives: In this study, the SP-38 (Diterpene Lactone derivative) was designed, synthesized from clerodane diterpene (lactone) isolated from Polyanthia longifolia var. pendula, and tested for anti-arthritic activity using the FCA-induced arthritic rat model.Materials and methods: This study examined the in vivo effects of SP-38 using three different doses (20, 10, and 5 mg/kg) by oral administration for 21 days from day 8 after 0.1 ml FCA sub-planter injection until day 28. Arthritis index, paw swelling, ankle diameter, body weight as well as biochemical, hematological, histopathological, and radiological parameters were examined.Results: Administered SP-38 reduced arthritis index, paw volume, and joint swelling compared to the arthritic control group. Accordingly, rats treated with SP-38 showed a remarkable increase in body weight and improved biochemical, hematological, histopathological, and radiological parameters. Furthermore, it reduced the increased production of CRP and RF while simultaneously decreasing ESR in all SP-38-treated rats. However, SP-38 showed promising liver protection by reducing elevated serum levels of liver and kidney function markers SGOT, SGPT, and ALP. Furthermore, splenic index, TNF-α, and IL-6 levels were significantly reduced compared to arthritic control rats at certain doses.Conclusion: The result of the present study concludes that SP-38 has significant anti-arthritic potential in FCA-induced arthritis in Wistar rats. SP-38 therefore showed promising anti-arthritic activity, as evidenced by attenuation of inflammation, inflammatory markers, and pro-inflammatory cytokine levels.","PeriodicalId":14495,"journal":{"name":"Iranian Journal of Basic Medical Sciences","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11266735/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141758774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of mesenchymal stem cells and polyvinyl alcohol-coated selenium nanoparticles on rats with Alzheimer-like phenotypes. 间充质干细胞和聚乙烯醇包裹的硒纳米粒子对阿尔茨海默样表型大鼠的影响

IF 2.1 4区医学

Iranian Journal of Basic Medical Sciences Pub Date : 2024-01-01 DOI: 10.22038/ijbms.2024.76242.16495

Siamak Shahidi, Sara Soleimani Asl, Bahareh Gholamigeravand, Simin Afshar, Nasrin Hashemi-Firouzi, Alireza Samzadeh-Kermani, Mahsa Majidi, Kimia Amiri

{"title":"Effect of mesenchymal stem cells and polyvinyl alcohol-coated selenium nanoparticles on rats with Alzheimer-like phenotypes.","authors":"Siamak Shahidi, Sara Soleimani Asl, Bahareh Gholamigeravand, Simin Afshar, Nasrin Hashemi-Firouzi, Alireza Samzadeh-Kermani, Mahsa Majidi, Kimia Amiri","doi":"10.22038/ijbms.2024.76242.16495","DOIUrl":"10.22038/ijbms.2024.76242.16495","url":null,"abstract":"Objectives: Mesenchymal stem cell (MSC) transplantation represents a promising approach for treating Alzheimer's disease (AD). These stem cells, however, have a short lifespan following transplantation into recipient animals. Selenium nanoparticles, due to their size, aid in drug delivery for brain disorders. This study investigated the therapeutic effect of MSCs and polyvinyl alcohol (PVA)-coated selenium nanoparticles (SeNPs) in a rat model of AD.Materials and methods: An Alzheimer-like phenotype was induced through intracerebroventricular (ICV) administration of streptozotocin (STZ). Rats were assigned to five groups: control, Alz (STZ; 3 mg/kg, 10 μl, ICV), Alz+stem cell (ICV transplantation), Alz+SeNP (0.4 mg/kg, orally), and Alz+stem cell+SeNPs. The ICV administration of STZ mimicked some aspects of AD in the Alz groups. SeNPs were administrated for 30 days following STZ administration. The novel object recognition (NOR) and passive avoidance learning (PAL) tests were used to evaluate cognition and memory. Oxidative stress biomarkers and brain-derived neurotrophic factor (BDNF) were assessed by biochemical analysis, ELISA kits, and Congo red staining, respectively.Results: The combined therapy of PVA-coated SeNPs and MSC transplantation was more effective in enhancing memory reacquisition compared to either SeNPs or MSCs alone. The use of stem cells in conjunction with PVA-coated SeNPs significantly boosted anti-oxidant capacity.Conclusion: The results suggest that the joint treatment with PVA-coated SeNPs and MSCs offers considerable neuroprotection against AD in animal models.","PeriodicalId":14495,"journal":{"name":"Iranian Journal of Basic Medical Sciences","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11366943/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142125698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thymoquinone: A comprehensive review of its potential role as a monotherapy for metabolic syndrome. 胸腺醌：全面回顾其作为代谢综合征单一疗法的潜在作用。

IF 2.1 4区医学

Iranian Journal of Basic Medical Sciences Pub Date : 2024-01-01 DOI: 10.22038/ijbms.2024.77203.16693

Kasimu Ghandi Ibrahim, Shuaibu Abdullahi Hudu, Amina Yusuf Jega, Ahmad Taha, Abdurrahman Pharmacy Yusuf, Dawoud Usman, Kehinde Ahmad Adeshina, Zayyanu Usman Umar, Trevor Tapiwa Nyakudya, Kennedy Honey Erlwanger

{"title":"Thymoquinone: A comprehensive review of its potential role as a monotherapy for metabolic syndrome.","authors":"Kasimu Ghandi Ibrahim, Shuaibu Abdullahi Hudu, Amina Yusuf Jega, Ahmad Taha, Abdurrahman Pharmacy Yusuf, Dawoud Usman, Kehinde Ahmad Adeshina, Zayyanu Usman Umar, Trevor Tapiwa Nyakudya, Kennedy Honey Erlwanger","doi":"10.22038/ijbms.2024.77203.16693","DOIUrl":"10.22038/ijbms.2024.77203.16693","url":null,"abstract":"Metabolic syndrome (MetS) is a widespread global epidemic that affects individuals across all age groups and presents a significant public health challenge. Comprising various cardio-metabolic risk factors, MetS contributes to morbidity and, when inadequately addressed, can lead to mortality. Current therapeutic approaches involve lifestyle changes and the prolonged use of pharmacological agents targeting the individual components of MetS, posing challenges related to cost, compliance with medications, and cumulative side effects. To overcome the challenges associated with these conventional treatments, herbal medicines and phytochemicals have been explored and proven to be holistic complements/alternatives in the management of MetS. Thymoquinone (TQ), a prominent bicyclic aromatic compound derived from Nigella sativa emerges as a promising candidate that has demonstrated beneficial effects in the treatment of the different components of MetS, with a good safety profile. For methodology, literature searches were conducted using PubMed and Google Scholar for relevant studies until December 2023. Using Boolean Operators, TQ and the individual components of MetS were queried against the databases. The retrieved articles were screened for eligibility. As a result, we provide a comprehensive overview of the anti-obesity, anti-dyslipidaemic, anti-hypertensive, and anti-diabetic effects of TQ including some underlying mechanisms of action such as modulating the expression of several metabolic target genes to promote metabolic health. The review advocates for a paradigm shift in MetS management, it contributes valuable insights into the multifaceted aspects of the application of TQ, fostering an understanding of its role in mitigating the global burden of MetS.","PeriodicalId":14495,"journal":{"name":"Iranian Journal of Basic Medical Sciences","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11366942/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142125708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Apatinib has anti-tumor effects and induces autophagy in lung cancer cells with high expression of VEGFR-2. 阿帕替尼具有抗肿瘤作用，并能诱导VEGFR-2高表达的肺癌细胞发生自噬。

IF 2.1 4区医学

Iranian Journal of Basic Medical Sciences Pub Date : 2024-01-01 DOI: 10.22038/ijbms.2024.74820.16246

Mingtao Liu, Hui Li

{"title":"Apatinib has anti-tumor effects and induces autophagy in lung cancer cells with high expression of VEGFR-2.","authors":"Mingtao Liu, Hui Li","doi":"10.22038/ijbms.2024.74820.16246","DOIUrl":"10.22038/ijbms.2024.74820.16246","url":null,"abstract":"Objectives: This study investigated the inhibitory effect of apatinib on lung cancer cells with high expression of vascular endothelial growth factor-2 (VEGFR-2) and on inducing cellular autophagy and drug resistance.Materials and methods: The expression of VEGFR-2 was detected using western blotting and RT-PCR. Cell proliferation was measured using the CCK8 and colony formation assays. The cell apoptosis rate was determined using flow cytometry and tunnel assay. Cellular autophagy was detected by measuring the expression of LC3-II using Western blotting and cellular immunofluorescence. The inhibitory effect of apatinib on lung cancer cells and transplanted tumors was observed after treatment with the autophagy inhibitor chloroquine.Results: Apatinib dose-dependently inhibited the proliferation of H1975 and H446 cells; it induced apoptosis via the PARP and caspase-3 pathways in H1975 and H446 cells and effectively inhibited the growth of transplanted tumors. Apatinib induced autophagy in a dose-dependent manner in H1975 and H446 cells. The inhibitory effect of apatinib on cells and the promotion of apoptosis were significantly enhanced after treatment with chloroquine. Immunohistochemistry showed that combining apatinib with chloroquine could reduce the expression of CD31 and Ki67 and increase the expression of caspase-3.Conclusion: Apatinib inhibits proliferation and induces apoptosis in H1975 and H1446 lung cancer cells with high VEGFR2 expression and autophagy in H1975 and H446 cells.","PeriodicalId":14495,"journal":{"name":"Iranian Journal of Basic Medical Sciences","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11459337/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142390524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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