Synergistic effect of cold atmospheric plasma and methylene blue loaded nano micelles on treating human glioblastoma cells: An in vitro and molecular dynamics study.

IF 2.1 4区 医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL
Elahe Ahmadi, Armin Imanparast, Mehdi Hoseini, Shahrokh Naseri, Samaneh Soudmand Salarabadi, Ameneh Sazgarnia
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引用次数: 0

Abstract

Objectives: One of the most recent cancer treatment methods is cold atmospheric plasma (CAP), which destroys cancer cells without affecting healthy cells. Also, the created photons in the CAP flame can be used to excite a proper photosensitizing agent (PS). Therefore, using nano micelle systems containing a proper photosensitizer may be beneficial in raising the treatment efficacy of CAP. In this study, we utilized molecular dynamics (MD) simulation to optimize a nano micellar system containing methylene blue to take advantage of the induced photodynamic effect of a CAP generator with helium gas on a glioblastoma cell line.

Materials and methods: Some micelle properties were first determined and optimized by MD with GROMACS software. Then, micelles containing methylene blue (Micelle-MB) and free methylene blue (MB) at various concentrations were prepared. Singlet oxygen dosimetry using 1,3-diphenylisobenzofuran (DPBF)was performed in the presence and absence of Micelle-MB and MB. Subsequently, the cytotoxicity of MB and Micelle-MB was evaluated on U87-MG cancer cells, and their half-maximal inhibitory concentrations (IC50) were determined. After 48 hr of treatment, the percentage of cell survival was determined using the MTT test. The experiments were repeated at least three times. The synergy index was selected to compare the results.

Results: Treatment with CAP and MB reduced the survival rate compared to the PS-free group with CAP. Results of singlet oxygen dosimetry showed that Micelle-MB might be more efficient in producing ROS. CAP treatment with Micelle-MB resulted in more cell death than free MB. In addition, cell viability decreased in Micelle-MB groups with increasing irradiation time in the three investigated irradiation times.

Conclusion: Using Micelle-MB in the CAP treatment improves treatment efficiency in the U87-MG cell line.

低温大气等离子体和亚甲基蓝负载纳米胶束对人胶质母细胞瘤细胞的协同作用:体外和分子动力学研究。
目的:冷大气等离子体(CAP)是最新的癌症治疗方法之一,它可以在不影响健康细胞的情况下杀死癌细胞。此外,在CAP火焰中产生的光子可以用来激发适当的光敏剂(PS)。因此,使用含有适当光敏剂的纳米胶束系统可能有助于提高CAP的治疗效果。在本研究中,我们利用分子动力学(MD)模拟来优化含有亚甲基蓝的纳米胶束系统,以利用氦气诱导CAP发生器对胶质母细胞瘤细胞系的光动力学效应。材料与方法:首先用GROMACS软件对胶束的一些性质进行了测定和优化。然后制备了不同浓度的含亚甲基蓝胶束(micell -MB)和游离亚甲基蓝胶束(MB)。在Micelle-MB和MB存在和不存在的情况下,用1,3-二苯基异苯并呋啶(DPBF)进行单重态氧剂量测定。随后,我们评估了MB和Micelle-MB对U87-MG癌细胞的细胞毒性,并测定了它们的半最大抑制浓度(IC50)。治疗48小时后,采用MTT试验测定细胞存活率。这些实验至少重复了三次。选取协同效应指标对结果进行比较。结果:与无ps组相比,CAP和MB组的存活率降低。单线态氧剂量测定结果显示,Micelle-MB可能更有效地产生ROS。与游离MB相比,Micelle-MB处理CAP导致更多的细胞死亡。此外,在三个研究的照射时间中,Micelle-MB组的细胞活力随着照射时间的增加而下降。结论:Micelle-MB用于CAP治疗可提高U87-MG细胞株的治疗效率。
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来源期刊
Iranian Journal of Basic Medical Sciences
Iranian Journal of Basic Medical Sciences MEDICINE, RESEARCH & EXPERIMENTAL-PHARMACOLOGY & PHARMACY
CiteScore
4.00
自引率
4.50%
发文量
142
审稿时长
6-12 weeks
期刊介绍: The Iranian Journal of Basic Medical Sciences (IJBMS) is a peer-reviewed, monthly publication by Mashhad University of Medical Sciences (MUMS), Mashhad, Iran . The Journal of "IJBMS” is a modern forum for scientific communication. Data and information, useful to investigators in any discipline in basic medical sciences mainly including Anatomical Sciences, Biochemistry, Genetics, Immunology, Microbiology, Pathology, Pharmacology, Pharmaceutical Sciences, and Physiology, will be published after they have been peer reviewed. This will also include reviews and multidisciplinary research.
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