Adropin在帕金森病大鼠模型中发挥神经保护作用。

IF 2.7 4区 医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL
Ayse Ozkan, Hande Parlak, Osman Sinen, Mehmet Bulbul, Mutay Aydin Aslan, Aysel Agar
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引用次数: 0

摘要

目的:探讨adropin在帕金森病大鼠模型中的保护作用机制。材料与方法:将3月龄雄性Wistar大鼠随机分为4组:i) Control, ii) Sham, iii) PD, iv) PD+Adropin。在纹状体注射6-羟多巴胺(6-OHDA) 7天后进行性能测试。采用免疫组化(IHC)方法检测黑质(SN)中酪氨酸羟化酶(TH)、G蛋白偶联受体19 (GPR19)、血管内皮生长因子受体2 (VEGFR2)的免疫反应性。用质谱法测定多巴胺水平。western blot检测糖原合成酶激酶3β (GSK3β)和pGSK3β (Ser9)蛋白水平。结果:我们的研究表明,adropin治疗显著改善了运动性能。与PD组相比,中枢adropin注射阻止了黑质多巴胺能神经元的丢失,并诱导了VEGFR2的表达,但没有诱导GPR19的表达。p-GSK3β/GSK3β比值各组间无显著差异。而PD+ adropin组注射adropin后SN内多巴胺水平升高。结论:adropin通过VEGFR2信号通路对黑质多巴胺能神经元具有保护作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Adropin exerts neuroprotection in an experimental rat model of Parkinson's disease.

Objectives: This study was planned to elucidate the mechanism of the protective effect of adropin in an experimental rat model of Parkinson's Disease (PD).

Materials and methods: Three-month-old male Wistar rats were randomly divided into four groups: i) Control, ii) Sham, iii) PD, and iv) PD+Adropin. The performance tests were performed seven days after the 6-Hydroxydopamine hydrochloride (6-OHDA) injection into the striatum. The immunoreactivities for tyrosine hydroxylase (TH), G protein-coupled receptor 19 (GPR19), and vascular endothelial growth factor receptor 2 (VEGFR2) were detected by immunohistochemistry (IHC) in the substantia nigra (SN). Dopamine levels were measured by mass spectrometry. Glycogen synthase kinase 3β (GSK3β) and pGSK3β (Ser9) protein levels were evaluated by western blot analysis.

Results: Our study demonstrated that motor performances were significantly improved by adropin treatment. Central adropin injection prevented the loss of nigral dopaminergic neurons and induced VEGFR2 expression but not GPR19 compared to the PD group. The ratio of p-GSK3β/GSK3β did not differ between groups. However, the level of dopamine in SN was increased with adropin injection in the PD+Adropin group.

Conclusion: Our findings reveal that adropin administration has a protective effect on nigral dopaminergic neurons and acts through the VEGFR2 signaling pathway.

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来源期刊
Iranian Journal of Basic Medical Sciences
Iranian Journal of Basic Medical Sciences MEDICINE, RESEARCH & EXPERIMENTAL-PHARMACOLOGY & PHARMACY
CiteScore
4.00
自引率
4.50%
发文量
142
审稿时长
6-12 weeks
期刊介绍: The Iranian Journal of Basic Medical Sciences (IJBMS) is a peer-reviewed, monthly publication by Mashhad University of Medical Sciences (MUMS), Mashhad, Iran . The Journal of "IJBMS” is a modern forum for scientific communication. Data and information, useful to investigators in any discipline in basic medical sciences mainly including Anatomical Sciences, Biochemistry, Genetics, Immunology, Microbiology, Pathology, Pharmacology, Pharmaceutical Sciences, and Physiology, will be published after they have been peer reviewed. This will also include reviews and multidisciplinary research.
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