International Microbiology最新文献

{"title":"Evaluation of the potential agricultural biocontrol of chitin-degrading Paenibacillus alvei FS1 through dual screening approaches and poison food agar assay.","authors":"Aina Sofea Mohd Shafullah, Muhamad Firdaus Syahmi Sam-On, Shuhaimi Mustafa, Aisyah Nabilah Ramlan, Mohd Termizi Yusof","doi":"10.1007/s10123-025-00678-z","DOIUrl":"10.1007/s10123-025-00678-z","url":null,"abstract":"<p><p>Agricultural pests, such as insects and fungi, contain chitin in their exoskeleton and cell walls, respectively. Chitin has become the target for biocontrol of these pests as an alternative to chemical pesticides. Thus, this study aims to isolate and characterise chitin-degrading bacteria from soil using dual screening approaches and poisoned food agar assay. Seven bacteria were isolated from soil samples. The selection of chitin-degrading bacteria was carried out using dual screening methods. Screening via agar well diffusion assay showed that isolate FS2 exhibited the largest halo zone diameter at 30.83 mm, followed by FS1. However, only isolate FS1 exhibited chitin degradation in the secondary screening via shrimp shell degradation. Thus, the antifungal activity of isolate FS1 was further investigated via the poisoned food agar assay involving cell-free supernatant (CFS) and bacterial culture against Curvularia lunata and Colletotrichum truncatum, which yielded the highest growth inhibition by CFS against C. lunata at 18.33 mm. Media culture screening exhibited that isolate FS1 in Trypticase Soy Broth showed the highest chitinase activity at 25.33 mm via agar well diffusion assay. Next, isolate FS1 at 3% of chitin yielded the highest chitinase activity at 350.30 U/mL. This isolate was further identified as Paenibacillus alvei with 99.79% accuracy via 16S rRNA gene sequencing and phylogenetic tree construction. These findings revealed that P. alvei FS1, isolated from soil, can degrade chitin and may be considered a potential biocontrol agent for insect and fungal pathogens in agriculture.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":"2161-2174"},"PeriodicalIF":2.3,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144234046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial and cytotoxic activity of bioactives from Paenibacillus dendritiformis isolated from Lonar Soda Lake, Maharashtra, India.","authors":"Pradip Bawane, Santosh Yele","doi":"10.1007/s10123-025-00682-3","DOIUrl":"10.1007/s10123-025-00682-3","url":null,"abstract":"<p><strong>Background: </strong>Constant research is being conducted to discover potent antibacterial and anticancer agents from natural sources like plants and microorganisms. Gram-positive Paenibacillus dendritiformis is a spore-forming bacterium with recognized industrial and economic significance in the production of important biomolecules applicable to the agricultural, pharmaceutical, and biotechnological industries.</p><p><strong>Objectives: </strong>The objective of this study was to investigate the antibacterial and cytotoxic potential of bioactives produced by P. dendritiformis isolated from Lonar Lake sediment.</p><p><strong>Methods: </strong>P. dendritiformis was isolated from Lonar Soda Lake and characterized using colony morphology, biochemical, and phylogenetic evaluations. The process was carried out using starch casein broth (SCB), and the bioactives were characterized by LC-MS/MS. The antibacterial assay was performed by the disc diffusion assay. The cytotoxic activity was performed by employing HeLa cell culture. To understand the molecular mechanism, oxidative stress markers, pro-inflammatory markers, and apoptotic markers were evaluated.</p><p><strong>Results: </strong>LC-MS/MS spectra reveal the presence of phytyl palmitate and phytol acetate. These bioactives from P. dendritiformis exhibit moderately significant to significant antibacterial and cytotoxic activity by substantially inhibiting the HeLa cell viability via MTT assay.</p><p><strong>Conclusion: </strong>The study demonstrates the antibacterial and cytotoxic potential of bioactive compounds from P. dendritiformis with significantly lower IC₅₀ values as compared to standard probes.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":"2217-2231"},"PeriodicalIF":2.3,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144325628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing cell viability and GABA production in fermented milk using fruit juice-coated alginate microencapsulated Lactiplantibacillus plantarum B7 during storage.","authors":"Sangkaran Pannerchelvan, Louise Lorna Lanne Jawlan, Helmi Wasoh, Mohd Shamzi Mohamed, Fadzlie Wong Faizal Wong, Mohamad Zulfazli Mohd Sobri, Rosfarizan Mohamad, Murni Halim","doi":"10.1007/s10123-025-00662-7","DOIUrl":"10.1007/s10123-025-00662-7","url":null,"abstract":"<p><p>Gamma-aminobutyric acid (GABA) is a non-protein amino acid with diverse health benefits, prompting interest in its incorporation into functional foods. In vitro probiotic characterization of Lactiplantibacillus plantarum B7, selected for its superior GABA production in a previous study, was performed before studying its microencapsulation using alginate coated with apple and pear juices to enhance cell viability and stability during storage in fermented milk. Both apple and pear juice-coated alginate microcapsules (AL-A + B7 and AL-P + B7) showed superior encapsulation, GIT condition tolerance, and release efficiency compared to alginate-only microcapsules. In comparison, free-cell L. plantarum B7 exhibited higher GABA production (2.59 ± 0.03 g/L), cell growth (8.96 ± 0.02 log CFU/mL), and the lowest pH (5.27 ± 0.06) at 48 h of fermentation. Among microencapsulated samples, AL-A + B7 showed the highest cell growth (8.93 ± 0.05 log CFU/g), GABA production (2.45 ± 0.05 g/L), and a lower pH (5.32 ± 0.06). During storage, AL-A + B7 retained higher viable cell counts (8.34 ± 0.13 log CFU/g) and improved GABA levels (3.90 ± 0.25 g/L) after 28 days, while free-cell samples showed a significant decline in cell count (from 8.96 ± 0.05 to 5.51 ± 0.13 log CFU/mL) and no significant improvement in GABA. These results highlight that apple juice-coated alginate (AL-A + B7) enhances the stability and viability of L. plantarum B7 during storage, while also promoting GABA production under storage conditions. These findings suggest its potential application in the development of functional foods with possible health benefits.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":"1857-1874"},"PeriodicalIF":2.3,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143995380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of cultivation conditions in hydrogel systems to enhance Chlorella vulgaris growth.","authors":"Guangtao Yang, Mingyang Li, Jinglin Zhang, Jindao Zhou, Wai Yan Cheah, Rosazlin Abdullah, Tau Chuan Ling","doi":"10.1007/s10123-025-00670-7","DOIUrl":"10.1007/s10123-025-00670-7","url":null,"abstract":"<p><p>Microalgae have gained significant attention as sustainable alternatives to traditional agriculture and energy resources. The cultivation efficiency of microalgae within hydrogel systems presents a promising avenue for spatially efficient bio-production. However, the optimum cultivation conditions of hydrogel cultivation systems have not been elucidated. This study focused on evaluating the hydrogel-based cultivation of Chlorella vulgaris in symbiosis with Bacillus Strain Salmah Ismail (SI) 139SI. It investigated the impact of hydrogel concentration, pH, light exposure, and system thickness on the growth and chlorophyll production of the algae. Our findings highlighted that, in coculture, a 7% (w/v) hydrogel concentration, pH of 7.4, a 12-h light/dark cycle at a hydrogel concentration of 7% but continuous light exposure under 5% hydrogel concentration, and a system thickness of 10 mm have provided the most favorable environmental conditions for the proliferation and chlorophyll production of C. vulgaris. These conditions significantly enhanced the biomass yields, suggesting that tailored hydrogel environments can substantially improve microalgae productivity.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":"1961-1977"},"PeriodicalIF":2.3,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144002105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dickeya zeae WH1 as sensor for cost-effective detection of pyocyanin produced by Pseudomonas aeruginosa.","authors":"Xiaojuan Tan, Guangyu Ju, Dandan Feng, Liuji Chen, Xiaodie Fan, Cancan Zhu, Minghui Li, Dongsheng Du","doi":"10.1007/s10123-025-00676-1","DOIUrl":"10.1007/s10123-025-00676-1","url":null,"abstract":"<p><p>Pyocyanin is a crucial virulence factor uniquely secreted by Pseudomonas aeruginosa. Hence, the rapid and selective detection of pyocyanin can reveal essential information about the pathogenesis of diseases caused by clinical isolates. Herein, we found that P. aeruginosa PAO1 exhibited remarkable antagonistic activity against Dickeya zeae WH1. Subsequently, its active compounds were purified by preparative high-performance liquid chromatography and HPLC, and anti-WH1 activity was determined by performing the minimum inhibitory concentration (MIC) assay. Only one active compound, pyocyanin, was obtained. Importantly, the linear quantification of pyocyanin to the biomass (OD600) of WH1 showed an R² value of 0.9908 ranging from 0.195 to 6.25 µg/mL, and pyocyanin concentration was accurately determined using this curve when P. aeruginosa was cultured in high-, medium-, and low-pyocyanin-producing media. Therefore, using D. zeae WH1 as a sensor for detecting pyocyanin and high-pyocyanin-producing isolates by high-throughput screening is a cost-effective and simple method.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":"2105-2113"},"PeriodicalIF":2.3,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144181593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Population dynamics of a bacterial consortium from a marine sediment of the Gulf of Mexico during biodegradation of the aromatic fraction of heavy crude oil.","authors":"Maria-Pilar Muñoz-Rivera, Fernando Martínez-Morales, Daniel Morales-Guzmán, Abraham Rivera-Ramírez, Ayixon Sánchez-Reyes, María R Trejo-Hernández","doi":"10.1007/s10123-025-00659-2","DOIUrl":"10.1007/s10123-025-00659-2","url":null,"abstract":"<p><p>In the marine environment, uncontained crude oil is dispersed and degraded by abiotic or biotic processes; native bacterial populations gradually adapt to integrate interspecific and intraspecific metabolic networks for efficient and dynamic utilization of xenobiotic substrates as carbon source. Aromatic compounds accumulate in marine sediments and bacterial populations at these sites play a crucial role in the mobilization of those complex molecules into the global geochemical cycles. The aim of this work was to use native bacteria from a marine sediment sample in the Gulf of Mexico to enhance the biodegradation of the aromatic fraction from a heavy crude oil, as the sole carbon source, during a 200-day microcosm experiment. This process involved the gradual increase of the aromatic fraction into the culture to promote bacterial enrichment; the increase in viable cells correlated well with a biodegradation pattern of the aromatic fraction at some points. Bacterial biodiversity, as revealed by metagenomic and microbiological approaches, indicates that bacterial groups are present at all fraction concentrations, but with changes in abundance, richness and dominance. Population dynamics revealed the presence of bacteria that modify emulsification and surface tension reduction values, which could promote the incorporation of the highly hydrophobic polyaromatic compounds into the culture aqueous phase for their biodegradation by hydrocarbonoclastic bacteria present. On the other hand, the presence of non-hydrocarbonoclastic bacteria probably is sustained by cross-feeding events involving sugars, amino acids, short carbon compounds, lipids produced by the former bacteria by co-metabolism of complex aromatic substrates, which are transformed into diverse biomolecules for biofilm development to promote a bacterial population dynamics adapted to this environment.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":"1875-1885"},"PeriodicalIF":2.3,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143969862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biodegradation of tetracycline by Cladosporium colombiae T1: performance and degradative pathway.","authors":"Yujun Ma, Jihong Wang, Su Leng","doi":"10.1007/s10123-025-00643-w","DOIUrl":"10.1007/s10123-025-00643-w","url":null,"abstract":"<p><p>The microbial degradation of tetracycline (TC) represents an effective bioremediation method. An effective TC-degrading strain of Cladosporium colombiae T1 was isolated from chicken manure using enrichment techniques. Response surface methodology was employed to ascertain the optimal conditions for removing TC by strain T1, identified as a temperature of 40.00 °C, solution pH of 6.92, TC concentration of 42.99 mg/L, and inoculum dose of 1.98%. The inhibitory effect of TC degradation products on Escherichia coli was investigated using the paper diffusion method. The results demonstrated that the toxicity of TC degradation products by T1 was lower than that of the parent compound. The shake-flask batch experiments showed that the biodegradation of TC was a synergistic effect of intra- and extracellular enzymes, with intracellular enzymes exhibiting greater efficacy in TC degradation (48.56%). LC-MS analysis identified ten potential biodegradation products, and biodegradation pathways were proposed. This study offers a theoretical foundation for the characterization and mechanistic investigation of TC degradation in the environment by Cladosporium colombiae T1.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":"1539-1551"},"PeriodicalIF":2.3,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143399098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Borrelidin M: a new borrelidin derivative obtained from Streptomyces rochei VL-16 exhibited potent antibacterial and antiproliferative properties.","authors":"Vinoth Vengadesan, Jagan Muniyandi, Neha Yadav, Premkumar G, Ajit Ramesh Sawant, Lakshmi Sankaranarayanan, Prashanth K, Toka Swu, Sudha Rani Sadras","doi":"10.1007/s10123-025-00673-4","DOIUrl":"10.1007/s10123-025-00673-4","url":null,"abstract":"<p><p>The lookout for novel metabolites to overcome the threat imposed by antibiotic resistant pathogens is on the rise globally. The present study reports on the identification and characterisation of a new borrelidin derivative from Streptomyces rochei VL-16 strain that was isolated from the soil samples of Osudu Lake in Puducherry. The active compound produced by Streptomyces rochei VL-16 strain was purified sequentially by thin layer, column and high performance liquid chromatography techniques. The purified compound was subsequently identified as borrelidin through Q-TOF-HR-LCMS, FTIR and UV-Vis spectroscopy techniques. Based on integration proton NMR signals and HR-ESI-MS peak pattern, the compound was further identified as a new derivative of borrelidin. Its 3D structure was optimised using density functional theory. The new derivative obtained in this study was designated as borrelidin M (C₂₈H₄₁NO_5, Mol. wt.471.3 g/mole). This derivative was found to exhibit potent inhibitory effects against the foodborne pathogens including Clostridium perfringens, Aeromonas hydrophila, Staphylococcus aureus, Listeria monocytogenes and Yersinia enterocolitica. Also, borrelidin M exhibited anticancer and anti-metastatic effects against A549 cell line with an IC₅₀ value of 17.5 μM. Collectively, these findings indicated promising biomedical applications of this new derivative of borrelidin.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":"2029-2046"},"PeriodicalIF":2.3,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144077994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phospholipid membrane permeabilization and leakage of cell content by surfactin-C₁₅ from novel B. subtilis B-11 strain: a computational and experimental analysis.","authors":"Asma Sani, Jia-Yi Li, Chaudhry Haider Ali, Hong-Ze Gang, Yi-Fan Liu, Shi-Zhong Yang, Bo-Zhong Mu","doi":"10.1007/s10123-025-00683-2","DOIUrl":"10.1007/s10123-025-00683-2","url":null,"abstract":"<p><p>Surfactin-C₁₅ produced by novel Bacillus subtilis B-11 strain has the potential to inhibit phytopathogens by permeabilizing their phospholipid cell membranes at the water/bilayer interface. This permeabilization leads to the disintegration of cell membranes, thus inhibiting growth, replication, and pathogenicity of phytopathogens. Model dipalmitoyl phosphocholine (DPPC) vesicles for pathogenic membranes were prepared by liposomal assays and used as representatives for phospholipid bilayer cell membrane. Results show that the hydrophobic fatty acid tail of surfactin-C₁₅ binds with the hydrophobic acyl chains of the DPPC bilayer membrane rather than with their hydrophilic head groups which tilt these acyl chains, causing the lipid headgroups to reorient forming pores in the membrane. AFM results show that structural disorderness increases at the nanoscale, specifically within the range of 0 to 3 nm. The fluorescence intensity of the encapsulated carboxyfluorescein probe increases in a concentration-dependent manner with surfactin-C₁₅ at 25 µM, 50 µM, and 75 µM, measured at a constant DPPC concentration of 10 µM, showing an emission increase from 200 to 800 nm. Heat flow decreases from DPPC: surfactin-C₁₅ (100:0) with a pretransition temperature of T_m 42.2 ± 0.1 (T_onset 40.9 ± 0.1) to DPPC: surfactin-C₁₅ (10:90) with a pretransition temperature of T_m 39.2 ± 0.1 (T_onset 36.9 ± 0.1). An increase in cholesterol concentration causes the size of DPPC vesicles to increase from 240 to 285 nm. These results confirm that larger vesicles exhibit higher interfacial activity compared to smaller vesicles, due to their greater surface area exposed to surfactin-C₁₅ at the membrane-water interface. This increase in vesicle size with cholesterol content is likely due to cholesterol's ability to modulate membrane fluidity and packing, resulting in altered vesicle morphology. The larger vesicles provide a more extensive contact area for surfactin-C₁₅ molecules at the membrane-water interface, facilitating stronger interactions that disrupt membrane integrity and enhance antimicrobial efficacy. This study suggests that surfactin-C₁₅ could be exploited for developing major biocontrol strategies in agriculture field.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":"2185-2206"},"PeriodicalIF":2.3,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144258073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacterial community composition and diversity associated with developmental stages of Anopheles subpictus.","authors":"Ankita Agrawal, Aishwarya B Acharya, Bijayalaxmi Sahu, Tapan Kumar Barik, Amiya Kumar Patel","doi":"10.1007/s10123-025-00688-x","DOIUrl":"10.1007/s10123-025-00688-x","url":null,"abstract":"<p><p>Malaria remains the deadliest vector-borne disease globally. Among different malaria vectors, Anopheles subpictus has been regarded as the potent vector across many Indian states. Microbiota in mosquitoes are critical determinants for their development, physiology, and vector competence, which differ significantly among mosquito species, life stages, and environmental parameters. Microbial profiles linked with various life stages of the emerging malaria vector, An. subpictus remain unexplored. In this study, 16S rRNA amplicon sequencing (V1-V9 regions) was used to explore and analyze the microbial community dynamics within and between life stages. A total of 287,077 bacterial reads were generated and distributed into 40 phyla, 75 classes, 160 orders, 334 families, 961 genera, and 2398 species. Diversity analyses revealed that bacterial community structure varied significantly across the three life phases (larvae, pupae, adult) of An. subpictus. The larval stage has higher species richness compared to subsequent developmental stages. The sequence-based taxonomic profiling revealed that Methylobacterium, Clostridium, Bradyrhizobium, Cytophaga, Roseateles, Mitsuaria, Sphingomonas and Wolbachia were the dominant bacterial genera across the different life stages. Moreover, Methylobacterium sp. (51.6%) was the most abundant taxa in the adult female stage, whereas the larval stage was mainly dominated by Cytophaga (15.2%). This research advances our understanding of bacterial community composition and dynamics across the developmental succession of An. subpictus. The finding pinpoints dominant bacterial candidates that could be harnessed to develop microbial-based control strategies aimed at limiting mosquito-borne diseases.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":"2257-2269"},"PeriodicalIF":2.3,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144333110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}