International Microbiology最新文献

{"title":"Biodiversity of strains belonging to the freshwater genus Aquirufa in a riparian forest restoration area in Salzburg, Austria, with a focus on the description of Aquirufa salirivi sp. nov. and Aquirufa novilacunae sp. nov.","authors":"Alexandra Pitt, Stefan Lienbacher, Johanna Schmidt, Meina Neumann-Schaal, Jacqueline Wolf, Hannah Wenng, Aharon Oren, Zoe Huber, Martin W Hahn","doi":"10.1007/s10123-025-00642-x","DOIUrl":"10.1007/s10123-025-00642-x","url":null,"abstract":"<p><p>During a citizen science project, four freshwater habitats in a riparian forest restoration area in Salzburg, Austria, were sampled. The primary aim was to obtain bacterial strains of the genus Aquirufa, a group of typical and widespread freshwater bacteria. Numerous pure cultures of Aquirufa strains could be obtained, three of them originating from the river Salzach, a newly created pond and the lake Ausee represented new species. Strain 1-SAACH-A3^T was characterized by a genome size of 3.2 Mbp and a G + C value of 38.4 mol% and encoded genes predicted for nitrate uptake and nitrous oxide utilization. Strains BAHN-186B^T and 2-AUSEE-184A6 were characterized by a genome size of 2.4 Mbp and a G + C value of 42.4 and 42.2 mol%, respectively, and encoded genes predicted for the light-harvesting rhodopsin system. Calculated whole-genome average nucleotide identity values with Aquirufa type strains resulted in a maximum value of 93.65% for comparison of strain 1-SAACH^T with the type strain of Aquirufa ecclesiirivi, which is slightly under the proposed threshold of species demarcation. The calculated gANI value comparing strains BAHN-186B^T and 2-AUSEE-184A6 revealed 95.76%, thus a value slightly above the threshold. Further analyses revealed that the three new strains represent two new species, proposed here as Aquirufa salirivi sp. nov. with type strain 1-SAACH-A3^T (= DSM 117800^T = JCM 37097^T) and Aquirufa novilacunae sp. nov. with type strain BAHN-186B^T (= DSM 118143^T = JCM 37099^T). Analyses of 123 publicly available metagenomes and a metagenome of the lake Ausee resulted in no detection of A. salirivi sp. nov. In contrast, A. novilacunae sp. nov. could be detected in 15 water samples of rivers, mainly from Asia, but also from North America and Australia. The analyses suggested that the species occurs in most of these samples in low relative abundance, detections derived from metagenomes of water samples from the river Yangtze in the subtropical zone could be interpreted as occurrence in higher abundances.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":"1635-1647"},"PeriodicalIF":2.3,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143440810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Purification, refolding, and pH-dependent stability evaluation of Zika virus EDIII protein.","authors":"Manjima Das, Vivek Kumar, Rishav Madhukalya, Anjali Saroj, Archana Rout, Anushka Upadhyay, Goutami Naidu, Krishna Mohan Poluri, Shailly Tomar, Dilip Kumar, Rajesh Kumar","doi":"10.1007/s10123-025-00679-y","DOIUrl":"10.1007/s10123-025-00679-y","url":null,"abstract":"<p><p>Zika virus (ZIKV) infection, primarily spread through mosquito bites, poses a significant threat as global temperatures continue to rise. While vector control remains the primary strategy for mitigation, the design and development of cost-effective vaccines are essential to prevent future outbreaks, particularly in low- and middle-income countries. In our study, we have produced recombinant ectodomain III (rEDIII) of ZIKV from the E. coli host expression system. Further, we optimized the effect of temperature and growth media on the expression of ZIKV rEDIII. Our study establishes a systematic protocol for extracting functional ZIKV rEDIII since the majority of the expressed protein goes into inclusion bodies (IBs). We have optimized the refolding process of rEDIII in buffers with varying pH levels and demonstrated the association between the buffer environment and the refolding efficiency of ZIKV rEDIII. Among these, Tris-HCl pH 8.8 buffer successfully restored the native conformation of ZIKV rEDIII, as confirmed by CD spectroscopy. We further confirmed the biological activity of refolded ZIKV rEDIII protein by inhibiting ZIKV entry into the host cells in an in vitro competitive viral inhibition assay. Our study proposes suitable conditions required for refolding bacterially expressed ZIKV rEDIII protein into its functionally active form with a median 50% inhibitory dose (ID50) value of 29 µg. The results of this investigation extend beyond the current study as the presented approach can be used to purify and refold other viral proteins expressed as IBs, thereby contributing to the advancement of diagnostic and therapeutic strategies for flavivirus infections.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":"2175-2184"},"PeriodicalIF":2.3,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144234047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phyto-and myco-chemical profiling, bioactivity, and in silico docking study of endophytic fungi and host-Setaria flavida.","authors":"R Nischitha, M B Shivanna","doi":"10.1007/s10123-025-00646-7","DOIUrl":"10.1007/s10123-025-00646-7","url":null,"abstract":"<p><p>Endophytic fungi are known to produce a wide array of bioactive compounds with significant pharmaceutical potential. This study focuses on exploring the antioxidant properties of endophytic fungi associated with Setaria flavida (Retz.) Veldkamp, in Karnataka, India. The fungi were isolated and characterized using morphological and internal transcribed spacer (ITS) sequence analyses. Five endophytic isolates were screened for antioxidant activity using spectrophotometric and electrochemical methods. The ethyl acetate extracts of Curvularia protuberata and methanolic extract of S. flavida showed strong antioxidant activity against DPPH, ABTS, H₂O₂, and nitric oxide radicals. Further, C. protuberata and S. flavida metabolites were characterized by Orbitrap High-Resolution Liquid Chromatography-Mass Spectrometer (OHR LC-MS). Molecular docking studies revealed compounds: (22E)-stigmasta-3,5,22-triene, andrographolide, gingerol, nobiletin, retusin, tangeritin, 1-benzoyl-4-(3,5-dibromobenzoyl) piperazine, 2-pyrazolin-5-ol, 5-tridecafluorohexyl-3-methyl-1-(3-pyridylcarbonyl), dexamethasone, griseofulvin, and S-benzyl-N[(benzyloxy)carbonyl] cysteinyltyrosine with potent antioxidant activity, inhibiting enzymes like xanthin oxidase (3NRZ) lipoxygenase (1N8Q) and myeloperoxidase (1DNU). The study suggests that these endophytic fungi and host grass could lead to the production of promising drugs in the pharmaceutical industry.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":"1687-1700"},"PeriodicalIF":2.3,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143491965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Agglutination of Escherichia coli, Clostridium perfringens, and Salmonella enterica through competitive exclusion using potassium chloride with gum arabic.","authors":"Akinori Uehara, Mayumi Maekawa, Yasuteru Sakamoto, Kazuki Nakagawa","doi":"10.1007/s10123-024-00625-4","DOIUrl":"10.1007/s10123-024-00625-4","url":null,"abstract":"<p><p>Bacterial infections causing necrotic enteritis and diarrhea pose a considerable economic loss to the animal industry. Using mannose oligosaccharides as competitive exclusion agents is an alternative method to antibiotic growth promoters; however, these materials are rapidly metabolized by gut microbiota, posing a challenge in sustaining their efficacy. The aim of this study was to identify an agglutination material that is effective against pathogens. Polysaccharides and salts were assessed using agglutination assays, microscopy, and zeta potential analysis. Gum arabic (GA) demonstrated strong agglutination against Escherichia coli and Salmonella enterica. Potassium chloride altered the cell form of Clostridium perfringens from rod-like to coccoid. When combined with GA, KCl effectively agglutinated all three bacterial species tested. Zeta potential analysis showed that agglutination resulted from bacteria, GA, and KCl interactions. Among various salts mixed with GA, KCl was found to strongly agglutinate C. perfringens upon its change into the coccoid form. Moreover, this combination has been shown to agglutinate mixtures of pathogens, such as C. perfringens and S. enterica. Thus, a combination of GA and KCl offers a potential solution to combat the pathogens associated with necrotic enteritis and diarrhea in animals.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":"1433-1448"},"PeriodicalIF":2.3,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142909700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Atmospheric CO₂ flux and planktonic food web relationships in temperate marsh systems: insights from in situ water measurements.","authors":"Xaus Lucila, Moncelon Raphaël, Mayen Jérémy, Bergeon Lauriane, Dubillot Bénédicte, Pineau Philippe, Emery Claire, Vagner Marie, Robin Francois-Xavier, Azémar Frédéric, Tackx Michèle, Dupuy Christine, Polsenaere Pierre","doi":"10.1007/s10123-025-00650-x","DOIUrl":"10.1007/s10123-025-00650-x","url":null,"abstract":"<p><p>While research has extensively investigated the dynamics of CO₂ water partial pressure (pCO₂) and planktonic food webs (PFWs) separately, there has been limited exploration of their potential interconnections, especially in marsh typologies. This study's objectives were to (1) investigated if pCO₂ and atmospheric CO₂ flux can be elucidated by PFW topologies, and (2) ascertain if these potential relationships are consistent across two distinct \"Blue Carbon\" ecosystems. Abiotic and biotic variables were measured in two contrasting wetlands at the Atlantic French coast: a saltwater (SM, L'Houmeau) and a freshwater marsh (FM, Tasdon). SM acted as a weak carbon source, with pCO₂ between 542 and 842 ppmv. Conversely, FM exhibited strong atmospheric CO₂ source or sink characteristics, varying with seasons and stations, with pCO₂ between 3201 and 114 ppmv. Five PFW topologies were linked to varying pCO₂ across the two ecosystems: three stable topologies ('biological winter', 'microbial', 'multivorous' PFW) exhibited consistently high pCO₂ values (FM: 971, 1136, 3020 ppmv; SM: 'biological winter' not observed, 842, 832 ppmv), while two transient topologies ('weak multivorous' and 'weak herbivorous') displayed lower and more variable pCO₂ values (FM: from 127 to 1402 ppmv; SM: from 638 to 749 ppmv). Seasonality emerged as an influencing factor for both pCO₂ dynamics and PFW. However, PFW in FM did not demonstrate a seasonal equilibrium state, potentially hindering a clearer understanding of the relationship between pCO₂ and PFW. This is the first documented association between PFW topologies and pCO₂ dynamics in \"Blue Carbon\" marsh environments.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":"1753-1769"},"PeriodicalIF":2.3,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143556880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization and genome analyses of the novel phages targeting extraintestinal Escherichia coli clones ST131 and ST410.","authors":"Md Shamsuzzaman, Yoon-Jung Choi, Shukho Kim, Jungmin Kim","doi":"10.1007/s10123-025-00686-z","DOIUrl":"10.1007/s10123-025-00686-z","url":null,"abstract":"<p><p>The rise of multidrug-resistant (MDR) extraintestinal pathogenic Escherichia coli (ExPEC) poses a severe healthcare threat, necessitating alternative treatment strategies such as bacteriophage therapy. In this study, four lytic phages (EC.W2-9, EC.W5-4, EC.W8-1, and EC.W14-2) were isolated from hospital wastewater and characterized for their efficacy against MDR ExPEC isolates. A total of 44 ExPEC isolates were analyzed, with 41% belonging to sequence type (ST) 131 and 59% to ST410, all exhibiting MDR phenotypes. Among these, 49% produced extended-spectrum β-lactamases (ESBLs), and 86% were identified as carbapenem-resistant E. coli (CREC). Phages EC.W2-9, EC.W8-1, and EC.W14-2 displayed high lytic activity against ST131 (≥ 90% lysis) but were significantly less effective against ST410 (≤ 30% lysis). Phage EC.W5-4 exhibited intermediate efficacy, lysing 78% of ST131 and 23% of ST410 isolates. Morphological and phylogenetic analyses classified EC.W2-9, EC.W5-4, and EC.W14-2 within the Tequatrovirus genus of the Straboviridae family, while EC.W8-1 belonged to the Kuravirus genus of the Podoviridae family. These phages demonstrated favorable biological properties, including short latent periods (10-25 min), high burst sizes (115-158 pfu/cell), and stability across a broad temperature (≤ 70 °C) and pH range (3-10). Genomic analysis (46,328-116,943 bp, 35.2-39.9% G + C content) confirmed the absence of virulence and antibiotic resistance genes. Furthermore, combined phage therapy effectively reduced planktonic and biofilm growth of MDR-ExPEC. These findings demonstrate the potential of these phages as effective therapeutic agents against MDR ExPEC, particularly ST131 and ST410, in combating antibiotic-resistant infections.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":"2233-2245"},"PeriodicalIF":2.3,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144333111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic properties of mercury‑tolerant yeast, Pichia kudriavzevii 1P4.","authors":"Rika Indri Astuti, Indra Maulana, Wulan Tri Wahyuni, Anja Meryandini","doi":"10.1007/s10123-025-00666-3","DOIUrl":"10.1007/s10123-025-00666-3","url":null,"abstract":"<p><p>Mercury contamination in commercial products poses serious risks to human health and the environment. The in vitro detection methods using spectroscopy approaches are expensive and have limited practical use. Yeast-based biosensors provide a more affordable and user-friendly alternative. Therefore, the exploration of mercury tolerance yeast is essential to support the sensor properties. In this study, we investigated the mercury tolerance of 10 yeast isolates. Pichia kudriavzevii 1P4 exhibited tolerance up to 1.5 mM HgCl₂ while showing slow growth phenotype as grown in 2 mM HgCl₂. This is the first report to show the ability of this genus of the yeast Pichia to cope with HgCl₂ stress. X-ray spectra showed Hg accumulation in yeast colonies grown in HgCl2, while none was detected in colonies grown without it. This data indicates the capability of 1P4 in the accumulation of Hg as one of the HgCl₂-stress tolerance mechanisms. Whole-genome sequencing of isolate 1P4, using the MGI DNBSEQ-G400 platform, revealed a genome size of 10.8 Mbp across five chromosomes, with a GC content of 38.97%. The largest portions of the genome are involved in translation, amino acid transport, metabolism, and protein modification, based on COG analysis. Key genes potentially contributing to mercury tolerance involve efflux/inorganic ion transport system (e.g., FieF, Acr3/B, and CzcO/D-clusters) and glutathione-associated oxidative stress response (e.g., BtuE and LysX, which encoded glutathione peroxidase and glutathione synthase). Those genes were predominant in COG category of inorganic ion transport and metabolism (P) and defense mechanisms (V). The genome of 1P4 was assembled into 4753 gene clusters, where 2222 of which were shared with the other species of yeast, including Pichia membranifaciens, P. kluyveri, P. inconspicua, S. cerevisiae, and C. albicans. Meanwhile, 921 gene clusters were shared among genera of Pichia spp., only. These findings highlight the genetic profile and mercury-tolerance mechanisms of isolate 1P4, supporting its potential application as a mercury biosensor or bioremediation agent.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":"2081-2094"},"PeriodicalIF":2.3,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144132391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Abiotic stressors influence saprolegniasis infection, causing mass mortality in Pangasianodon hypophthalmus and Labeo rohita under cage culture in Eastern India.","authors":"Sanjaykumar Karsanbhai Rathod, Manoharmayum Shaya Devi, Asit Kumar Bera, Samikshya Mishra, Basanta Kumar Das, Ritesh Shantilal Tandel, Sanjib Kumar Manna","doi":"10.1007/s10123-025-00721-z","DOIUrl":"https://doi.org/10.1007/s10123-025-00721-z","url":null,"abstract":"<p><p>Saprolegniasis is one of the most devastating fish diseases that cause fish mortality in aquaculture in winter season, leading to economic loss to the farmers. The mycotic infection in fish occurred during the winter season in inland open water bodies due to adverse environmental factors, poor culture practices, high stocking densities, rough handling, and physiological changes associated with immune suppression. In the present investigation, we studied the environmental factors for disease progress in Pangasianodon hypophthalmus and Labeo rohita, characterization of oomycetes, and screening of potential antifungal agents against fish pathogenic oomycetes. Mortality and infection patterns have a relationship with water temperature in the present investigation. We noted that co-habitat infection was found in L. rohita and caused mortality. The presence of certain abiotic factors and susceptible host species led to the outbreak of disease in cage culture. The morphological and molecular identification of the etiological agent was carried out, and the PCR amplicon sequence of the ITS gene fragment showed similarities with Saprolegnia aenigmatica. The optimum temperature required by S. aenigmatica was between 20 and 25 °C for hyphae growth. In vitro assessment revealed that fluconazole (FLZ), boric acid (BA), and potassium permanganate (KMnO4) were unable to control zoospore colonization at higher concentrations. Clotrimazole and hydrogen peroxide inhibited colonization in 10 ppm and 100 ppm, respectively. The present investigation identified and confirmed the causative agent of saprolegniasis outbreak with associated environmental factors and approached to develop antifungal therapeutics to combat infection in the inland open-water bodies.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145199428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combating multidrug-resistant uropathogenic E. coli using lytic phages, enhancing antibiotic synergy and inhibiting biofilms.","authors":"Md Shamsuzzaman, Yoon-Jung Choi, Shukho Kim, Jungmin Kim","doi":"10.1007/s10123-025-00727-7","DOIUrl":"https://doi.org/10.1007/s10123-025-00727-7","url":null,"abstract":"<p><p>The global emergence of multidrug-resistant Escherichia coli (MDR E. coli), driven by excessive antibiotic use and environmental persistence, poses a major threat to public health. Bacteriophages (phages) have garnered renewed interest as targeted biocontrol agents against such pathogens. In this study, we isolated and characterized two lytic phages, EC.W1-1 and EC.W15-3, specifically targeting diverse sequence types (STs) of MDR E. coli. Both phages belong to the family Straboviridae and genus Tequatrovirus. They demonstrated remarkable stability across pH 2-10 (4 h) and temperatures below 80 °C (1 h), and exhibited potent in vitro lytic activity at various multiplicities of infection (MOIs, 10-0.001). One-step growth curves revealed short latent periods (10-15 min) and moderate burst sizes (64-83 PFU/cell). Genome analysis showed sizes ranging from 37,736 to 123,792 bp, with G + C contents of 35.6%-37.2%, and no virulence or antibiotic resistance genes were detected, underscoring their safety profile. Functional annotation indicated coding sequences related to structural proteins, DNA replication, transcription, repair, and lytic functions. Importantly, the phages maintained partial activity in mouse and human serum and exhibited intracellular persistence in murine macrophages, supporting their biological stability. Notably, combined treatment of phages with sub-lethal antibiotic doses effectively inhibited extended-spectrum β-lactamase (ESBL)-producing and carbapenem-resistant E. coli (CREC). Moreover, both phages efficiently disrupted biofilms formed by different MDR E. coli STs. Collectively, these findings highlight the strong therapeutic potential of EC.W1-1 and EC.W15-3, offering a promising alternative or adjunct to antibiotics in combating MDR E. coli infections.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145199443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization and statistical lipid optimization of an oleaginous soil fungus, Gongronella butleri HMA-10: a promising novel source for sustainable lipid production.","authors":"Adel Eltoukhy, Hassan Mohamed, M A Abo-Kadoum, Asmaa S Ramadan, Hina Khalid, Bushra Iqbal, Chao Du, Yuanda Song","doi":"10.1007/s10123-025-00718-8","DOIUrl":"https://doi.org/10.1007/s10123-025-00718-8","url":null,"abstract":"<p><p>Oleaginous fungi, as a vital part of the microbiota in naturally diverse ecosystems, represent a reservoir for biomass-based lipid production. The challenge is the selection of promising strains with cost-effective operation for multiple applications. In the current study, 12 fungal strains were isolated and screened for their oleaginicity. All strains were fermented and accumulated over 20% lipids in their cell dry weight (CDW). After the preliminary screening, one strain was genetically identified as Gongronella butleri HMA-10 due to its maximum biomass (15.8 ± 0.8 g/L) and lipid (40.6 ± 1.7%) yields. Moreover, the HMA-10 was subjected to optimization of various parameters with the help of response surface methodology (RSM) for maximum lipid accumulation. The selected strain was fermented on fructose associated with a combination of sodium nitrite and yeast extract, exhibiting optimum nutritional conditions with a C/N ratio of (40:1). Cultivation conditions (4 days, 27.5 ℃, and pH of 7.0) resulted in the highest CDW and lipid content. Scaling up of HMA-10 in bioreactor fermentation under optimized conditions significantly induced biomass and lipid content (19.5 g/L and 53%), compared with shake flask fermentation (15.8 ± 0.8 g/L and 40.6 ± 1.7%), respectively. Fatty acid (FA) profile was confirmed by gas chromatography (GC) analysis, and the results revealed that oleic acid (OA) was the predominant (46.91%), while palmitic acid (PA), linoleic acid (LA), and γ-linolenic acid (GLA) were found in significant quantities (21.13, 9.67, and 8.06%), respectively. This study employed for the first time G. butleri HMA-10 as a promising candidate for high lipid accumulation with a potential second-generation biodiesel production in large-scale industrialization.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145185967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}