International Microbiology最新文献

Effect of low-frequency alternating magnetic field on exopolysaccharide production and antioxidant capacity of Pleurotus citrinopileatus by submerged fermentation. 低频交变磁场对浸没发酵法柠檬多糖产量和抗氧化能力的影响

IF 2.3 4区生物学

International Microbiology Pub Date : 2024-10-18 DOI: 10.1007/s10123-024-00604-9

Jingya Qian, Dazhou Lu, Zixuan Zhang, Di Chen, Feng Zhao, Shuhao Huo, Feng Wang, Haile Ma, Juan Kan

{"title":"Effect of low-frequency alternating magnetic field on exopolysaccharide production and antioxidant capacity of Pleurotus citrinopileatus by submerged fermentation.","authors":"Jingya Qian, Dazhou Lu, Zixuan Zhang, Di Chen, Feng Zhao, Shuhao Huo, Feng Wang, Haile Ma, Juan Kan","doi":"10.1007/s10123-024-00604-9","DOIUrl":"https://doi.org/10.1007/s10123-024-00604-9","url":null,"abstract":"The objective of this study was to investigate the effect of low-frequency alternating magnetic field (LF-AMF) on the production of extracellular polysaccharide (EPS) by submerged fermentation of Pleurotus citrinopileatus. The fermentation conditions optimized by the central composite design method were as follows: fermentation time of 6.18 days, temperature of 28.28 °C, shaking speed of 149.04 r/min, and inoculum amount of 8.43%. Under these conditions, a LF-AMF was applied to the submerged fermentation of P. citrinopileatus. When the intensity of LF-AMF was 40 Gs, the initial intervention time was 24 h after inoculation, and the treatment time was 6 h at one time, the mycelial biomass of P. citrinopileatus increased by 11.30%, and the EPS yield increased by 23.09% compared with the fermentation without LF-AMF treatment. The morphology of mycelium after LF-AMF treatment was observed by scanning electron microscopy. It was found that the surface of mycelium was wrinkled, and the structure of mycelium was loose, which might be more conducive to the production of EPS. Mycelium diameter decreased, and ATPase activity increased, indicating that LF-AMF had a positive effect on the production of EPS by P. citrinopileatus fermentation. Moreover, LF-AMF could improve the permeability of the mycelial cell membrane, facilitate the exchange of intracellular and extracellular substances, and increase the metabolic capacity of P. citrinopileatus. In vitro antioxidant test of EPS showed that LF-AMF treatment also improved its antioxidant capacity.","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142465463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and characterization of Pantoea ananatis and P. agglomerans in quinoa: P. ananatis as a potential fungal biocontroller and plant growth promoter. 藜麦中 Pantoea ananatis 和 P. agglomerans 的分离和特征：P. ananatis 是一种潜在的真菌生物控制剂和植物生长促进剂。

IF 2.3 4区生物学

International Microbiology Pub Date : 2024-10-16 DOI: 10.1007/s10123-024-00608-5

Jeimy L Valbuena-Rodríguez, Ingrid Fonseca-Guerra, Claudia Buitrago-Yomayusa, Alexander Puentes-S, Martha Elizabeth Benavides Rozo

{"title":"Isolation and characterization of Pantoea ananatis and P. agglomerans in quinoa: P. ananatis as a potential fungal biocontroller and plant growth promoter.","authors":"Jeimy L Valbuena-Rodríguez, Ingrid Fonseca-Guerra, Claudia Buitrago-Yomayusa, Alexander Puentes-S, Martha Elizabeth Benavides Rozo","doi":"10.1007/s10123-024-00608-5","DOIUrl":"https://doi.org/10.1007/s10123-024-00608-5","url":null,"abstract":"Chenopodium quinoa, globally recognized as quinoa, stands out as one of the cereals with the highest nutritional value native to the Americas. It is cultivated in the Andes Mountain range, and Colombia is no exception, with the Boyacá department emerging as a significant quinoa-producing region. The quinoa ecosystem harbors a rich array of microorganisms within its rhizosphere. In this current study, nitrogen-fixing and phosphate-solubilizing isolates AM-0261 (Pantoea ananatis) and AM-0263 (Pantoea agglomerans) were sourced from rhizospheric soil samples of quinoa. These isolates were subjected to biochemical characterization and identification through PCR analysis and Sanger sequencing targeting a partial sequence of the 16 s region of the rRNA. To assess their potential as plant growth-promoting rhizobacteria (PGPR), taking into consideration that P. ananatis is an IAA producer, greenhouse-based bioassays were conducted using seedlings. Additionally, dual culture assays were employed to showcase their antagonistic capabilities against primary beneficial and phytopathogenic fungi associated with quinoa cultivation in the region. The results underscore the remarkable potential of P. ananatis as a PGPR and a biocontrol agent against quinoa's phytopathogenic fungi. This study represents the pioneering exploration of the interaction between these two bacterial strains with quinoa rhizosphere tissue. In addition, the isolate of P. annatis (AM-0261) stands out, which presents phosphate solubilization capacity, nitrogen fixation, antagonistic capacity, and IAA production, characteristics that make it a promising strain for its use for the management of diseases of fungal origin, and in the future, it could be useful in reducing the use of chemical fertilizers.","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142465464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Community composition and co-occurrence of free-living and particle-attached bacteria in the source region of the Ganges and Brahmaputra Rivers. 恒河和雅鲁藏布江源头地区自由生活细菌和颗粒附着细菌的群落组成和共存情况。

IF 2.3 4区生物学

International Microbiology Pub Date : 2024-10-14 DOI: 10.1007/s10123-024-00607-6

Namita Paudel Adhikari, Subash Adhikari, Komal Raj Rijal

{"title":"Community composition and co-occurrence of free-living and particle-attached bacteria in the source region of the Ganges and Brahmaputra Rivers.","authors":"Namita Paudel Adhikari, Subash Adhikari, Komal Raj Rijal","doi":"10.1007/s10123-024-00607-6","DOIUrl":"https://doi.org/10.1007/s10123-024-00607-6","url":null,"abstract":"Bacteria have two trophic lifestyles in aquatic ecosystems, i.e., free-living (FL) and particle-attached (PA), with different but essential ecological roles. However, relevant knowledge is still dearth in the upstream source region of the Himalayan Rivers. Thus, we emphasized a comparative study on community composition, co-occurrence, and geographic distribution of the FL and PA bacteria and the effect of environmental factors in the source region of the Ganges and Brahmaputra Rivers. PA bacteria relative to FL harbored a significantly higher local diversity, richness, and evenness. A significantly higher abundance of Betaproteobacteria, Verrucomicrobiota, and Planctomycetota in PA trophic lifestyle and Gammaproteobacteria and Actinomycetota in FL tropic lifestyle and indicator OTUs belonging to related taxa were observed. The spatial variation of the FL and PA bacterial communities was most significantly impacted by dispersal limitation as a discrete factor. Among the environmental parameters, the total nitrogen (TN) was found to be a significant (P < 0.001) driver of the variation in PA communities. Meanwhile, particulate organic carbon (POC) and TN considerably explained the variation of FL communities. A significant correlation (P < 0.001) of TN with dominant bacterial taxa (Pseudomonadota, Actinomycetota, and Verrucomicrobiota) and FL and PA indicator OTUs associated with these taxa further confirmed nitrogen as the limiting nutrient in the source region of the Ganges and Brahmaputra Rivers. The co-occurrence network topological characteristics showed that the PA network was more stable than the FL network, which was more complicated and unstable. Thus, it can be speculated that FL communities relative to PA are more vulnerable to shifting upon disturbances.","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142465462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening and application of functional autochthonous starter culture from cured meat, which can reduce nitrite content. 筛选和应用可降低亚硝酸盐含量的腌肉功能性自生启动培养物。

IF 2.3 4区生物学

International Microbiology Pub Date : 2024-10-14 DOI: 10.1007/s10123-024-00606-7

Qiuhui Zhang, Jialong Shen, Gaoge Meng, Han Wang, Chang Liu, Chaozhi Zhu, Gaiming Zhao

{"title":"Screening and application of functional autochthonous starter culture from cured meat, which can reduce nitrite content.","authors":"Qiuhui Zhang, Jialong Shen, Gaoge Meng, Han Wang, Chang Liu, Chaozhi Zhu, Gaiming Zhao","doi":"10.1007/s10123-024-00606-7","DOIUrl":"https://doi.org/10.1007/s10123-024-00606-7","url":null,"abstract":"Cured meat is a fermented meat product from the traditional Chinese culture made by natural fermentation. In this study, five bacteria strains were screened from cured meat using 16S rDNA technology, and a functional local starter was selected, which was applied to the production of cured meat to standardize the production of cured meat and improve the quality of cured meat. By studying the fermentation characteristics of strain these strains, this study found that the fermentation characteristics of L. mesenteroides and S. lactis are ideal. L. mesenteroides and S. lactis were used as starter cultures in fermented bacon. Then, this study compared the quality of fermented beef with Sichuan bacon, Hunan bacon, and Xinyang bacon. The results suggested that L. mesenteroides and S. lactis can improve the sensory and texture properties of the products and reduce the moisture content, water activity, pH value, and protein content of fermented beef products. More importantly, L. mesenteroides can significantly reduce the nitrite content (25.34%) and nitrosamine content (29.69%) in fermented beef, which provides an excellent guarantee for the safety of cured meat. In this study, a functional fermentation strain-L. mesenteroides could degrade the nitrite content of fermented meat products and improve their sensory and textural properties-was screened to provide some reference value for the later development of functional strains suitable for fermented meat products.","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142465465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Viability of Lactobacillus acidophilus in acidophilus milk during frozen storage and its potential to lower cholesterol: an In vivo study. 嗜酸乳杆菌牛奶中嗜酸乳杆菌在冷冻储存期间的活力及其降低胆固醇的潜力：一项体内研究。

IF 2.3 4区生物学

International Microbiology Pub Date : 2024-10-14 DOI: 10.1007/s10123-024-00605-8

Yurliasni Yurliasni, Elmy Mariana, Yusdar Zakaria, Zuraida Hanum

{"title":"Viability of Lactobacillus acidophilus in acidophilus milk during frozen storage and its potential to lower cholesterol: an In vivo study.","authors":"Yurliasni Yurliasni, Elmy Mariana, Yusdar Zakaria, Zuraida Hanum","doi":"10.1007/s10123-024-00605-8","DOIUrl":"https://doi.org/10.1007/s10123-024-00605-8","url":null,"abstract":"The viability and functional activity of lactic acid bacteria (LAB), such as Lactobacillus acidophilus in a fermented milk product is important. One of the functional activities of the LAB in fermented milk is the ability of the LAB to positively impact the human health. This study aimed to determine the effect of frozen storage on the fermented milk, namely acidophilus milk, based on the nutritional milk quality, the viability of L. acidophilus, and its potential to lower cholesterol levels in Wistar rats. The parameters measured was including milk quality (mainly pH, protein content, lactic acid levels, and syneresis), L. acidophilus bacterial viability in frozen storage (for 1, 2, and 3 months), and some biological assays to evaluate the potential of milk in lowering blood cholesterol levels in Wistar rats. The result of this study suggests that acidophilus milk quality can be maintained in frozen storage for two months, and it had lactic acid levels of 1.07%, pH of 4.08, and protein levels of 3.33%. Giving acidophilus milk to Wistar rats for 15 days could reduce the cholesterol level continuously until 30 days of treatment. Therefore, this study proves that acidophilus milk quality can be maintained very well in frozen storage, and its functional properties to lower the cholesterol level of Wistar rats can be achieved after two weeks of consumption.","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142465466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic enhancement of polycyclic aromatic hydrocarbon degradation by Arthrobacter sp. SZ-3 and Pseudomonas putida B6-2 under high Tween80 concentration: mechanisms and efficiency. 在高浓度吐温80条件下，节杆菌SZ-3和假单胞菌B6-2协同增强多环芳烃降解：机理与效率

IF 2.3 4区生物学

International Microbiology Pub Date : 2024-10-09 DOI: 10.1007/s10123-024-00603-w

Mingle Zhang, Zhimin He, Xiaoyi Xu, Fan Ji, Bin Wang

{"title":"Synergistic enhancement of polycyclic aromatic hydrocarbon degradation by Arthrobacter sp. SZ-3 and Pseudomonas putida B6-2 under high Tween80 concentration: mechanisms and efficiency.","authors":"Mingle Zhang, Zhimin He, Xiaoyi Xu, Fan Ji, Bin Wang","doi":"10.1007/s10123-024-00603-w","DOIUrl":"https://doi.org/10.1007/s10123-024-00603-w","url":null,"abstract":"This study investigates the advantages of combined microbial degradation of polycyclic aromatic hydrocarbons (PAHs) in reducing the inhibitory effects of high-concentration eluents commonly used in soil washing. A microbial synergistic strategy was proposed using Arthrobacter sp. SZ-3 and Pseudomonas putida B6-2 as the key bacteria in the presence of Tween 80. The results show that in systems with Tween 80, the SZ-3 strain exhibits a strong capacity to degrade three types of PAH compounds, while the B6-2 strain follows multiple degradation pathways. Mixed bacteria achieved degradation rates 60.70% higher than single bacteria at varying concentrations of Tween 80. Additionally, the average growth rates of mixed bacteria increased by 1.17-1.37 times, aligning with the changes in the functional group. Protein activity detection within each degradation system corresponded with growth quantity and the cyclic variation characteristics of ETS enzyme activity. Notably, the ETS activity of mixed bacteria was 150% higher than that of single bacteria. At a Tween 80 concentration of 500 mg/L, the degradation rates of PAHs (Phe, Flu, Pyr) by mixed bacteria were significantly higher than those by single bacteria. The catechol 1,2-dioxygenase activity of mixed bacteria was 2.30 times higher than that of single bacteria. While Tween 80 did not alter the PAH degradation pathways, it significantly influenced the accumulation amount and duration of the characteristic intermediate product. This provides a reference for the remediation of recalcitrant pollutants under conditions involving high-concentration surfactants.","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142390507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Calonectria eucalyptorum sp. nov., a new leaf blight pathogen of Eucalyptus from India. Calonectria eucalyptorum sp.

IF 2.3 4区生物学

International Microbiology Pub Date : 2024-10-07 DOI: 10.1007/s10123-024-00602-x

Nitika Negi, Ramkrishna, Rajendra K Meena, Maneesh S Bhandari, Shailesh Pandey

{"title":"Calonectria eucalyptorum sp. nov., a new leaf blight pathogen of Eucalyptus from India.","authors":"Nitika Negi, Ramkrishna, Rajendra K Meena, Maneesh S Bhandari, Shailesh Pandey","doi":"10.1007/s10123-024-00602-x","DOIUrl":"https://doi.org/10.1007/s10123-024-00602-x","url":null,"abstract":"In this study, Calonectria eucalyptorum sp. nov. is described from the blighted leaves of Eucalyptus in India using morphological and multi-locus phylogenetic analyses. The new species belongs to the Calonectria cylindrospora species complex, and its unique microscopic features and DNA sequence information enable clear separation from the 12 currently accepted species in this complex. Conidia of the new taxon are slightly longer than those of its phylogenetic neighbors. Additionally, this species produces central as well as lateral stipe extensions, which is a feature not known for the other members of the C. cylindrospora species complex. Analyses of the combined partial calmodulin, histone, translation elongation factor-1α, and β-tubulin gene regions revealed a distinct phylogenetic position for C. eucalyptorum. Recombination analysis provided additional support for the new species hypothesis. Koch's postulates for the new taxon as a foliar pathogen of Eucalyptus were fulfilled. The discovery of novel and pathogenic Calonectria species is important because it sheds light on species diversity, potential threats, and disease control.","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142380846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Carlos Asensio and the dawn of molecular microbial ecology. 卡洛斯-阿森西奥和分子微生物生态学的曙光。

IF 2.3 4区生物学

International Microbiology Pub Date : 2024-10-03 DOI: 10.1007/s10123-024-00596-6

Víctor de Lorenzo, Fernando Baquero, Alfredo Aguilar

{"title":"Carlos Asensio and the dawn of molecular microbial ecology.","authors":"Víctor de Lorenzo, Fernando Baquero, Alfredo Aguilar","doi":"10.1007/s10123-024-00596-6","DOIUrl":"https://doi.org/10.1007/s10123-024-00596-6","url":null,"abstract":"At near 50 years of the discovery of microcins, this article highlights the pivotal-but under-recognised-influence of Spanish biochemist Carlos Asensio (1925-1982) in contemporary microbiology, featuring the epistemological, sociological, and cultural impact of his scientific achievements. At a time when the intestinal microbiome is central to current biomedical research, it is due to emphasise his role in the establishment of new scientific fields that are now considered fundamental. Despite his premature death at the peak of his conceptual and experimental creativity, many of his ideas about microbial communication in complex communities inspired a generation of researchers and opened new topics reach to this day. Asensio was also a trailblazer in Spain, advocating for fundamental research within the socio-economic context of his time. He foresaw the shift towards what is now termed the knowledge-based bioeconomy, recognised the need for multidisciplinary research teams, and advocated integration science into societal and political agendas. These facets became evident during his research on microcins, low molecular weight bioactive compounds produced by enterobacteria. These molecules were hypothesised as mediators of microbial interactions in the human gut and were considered potential new antibiotics and even antitumoral agents. His research mobilised young talent and attracted unprecedented resources in Spain during the late 1970s-early 1980s. It underscored the medical value of microbial ecology and exemplified the benefits of collaboration between academia and industry. Asensio played a pivotal role in the emergence of molecular microbial ecology as a research discipline and its foundational and applied significance in biotechnology.","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142365208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating the antifungal potential of genetically modified hybrid chitinase enzymes derived from Bacillus subtilis and Serratia marcescens. 研究源自枯草芽孢杆菌和肉豆蔻沙雷氏菌的转基因杂交几丁质酶的抗真菌潜力。

IF 2.3 4区生物学

International Microbiology Pub Date : 2024-10-02 DOI: 10.1007/s10123-024-00591-x

Nahla Alsayd Bouqellah, Lina Jamil Mohamed Abdel-Hafez, Islam Yousif Mostafa, Ahmed Hassan Ibrahim Faraag

{"title":"Investigating the antifungal potential of genetically modified hybrid chitinase enzymes derived from Bacillus subtilis and Serratia marcescens.","authors":"Nahla Alsayd Bouqellah, Lina Jamil Mohamed Abdel-Hafez, Islam Yousif Mostafa, Ahmed Hassan Ibrahim Faraag","doi":"10.1007/s10123-024-00591-x","DOIUrl":"https://doi.org/10.1007/s10123-024-00591-x","url":null,"abstract":"Chitinases are glycosyl hydrolase enzymes that break down chitin, an integral component of fungal cell walls. Bacteria such as Bacillus subtilis and Serratia marcescens produce chitinases with antifungal properties. In this study, we aimed to generate hybrid chitinase enzymes with enhanced antifungal activity by combining functional domains from native chitinases produced by B. subtilis and S. marcescens. Chitinase genes were cloned from both bacteria and fused together using overlap extension PCR. The hybrid constructs were expressed in E. coli and the recombinant enzymes purified. Gel electrophoresis and computational analysis confirmed the molecular weights and isoelectric points of the hybrid chitinases were intermediate between the parental enzymes. Antifungal assays demonstrated that the hybrid chitinases inhibited growth of the fungus Fusarium oxysporum significantly more than the native enzymes and also showed fungicidal activity against Candida albicans, Alternaria solani, and Rhizoctonia solani. The results indicate that hybrid bacterial chitinases are a promising approach to engineer novel antifungal proteins. This study provides insight into structure-function relationships of chitinases and strategies for generating biotherapeutics with enhanced bioactive properties. These hybrid chitinases result in a more potent and versatile antifungal agent.","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142361486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated processes for olive mill wastewater treatment and its revalorization for microalgae culture. 橄榄油厂废水处理综合工艺及其对微藻类培养的再评价。

IF 2.3 4区生物学

International Microbiology Pub Date : 2024-10-02 DOI: 10.1007/s10123-024-00600-z

Rihab Hachicha, Jihen Elleuch, Pascal Dubessay, Ridha Hachicha, Slim Abdelkafi, Philippe Michaud, Imen Fendri

{"title":"Integrated processes for olive mill wastewater treatment and its revalorization for microalgae culture.","authors":"Rihab Hachicha, Jihen Elleuch, Pascal Dubessay, Ridha Hachicha, Slim Abdelkafi, Philippe Michaud, Imen Fendri","doi":"10.1007/s10123-024-00600-z","DOIUrl":"https://doi.org/10.1007/s10123-024-00600-z","url":null,"abstract":"The olive oil industry generates 30 million cubic meters of olive mill wastewaters (OMWWs) annually. OMWWs are a major environmental concern in the Mediterranean region due to their high organic matter content, suspended solids, unpleasant odor, and dark color. The application of primary treatments such as coagulation-flocculation, adsorption, and hybrid systems combining coagulation-flocculation with adsorption has enabled to remove part of the organic matter, color, turbidity, and growth-inhibiting compounds from OMWWs. Among these methods, the hybrid system combining activated carbon and chitosan has proven to be the best removal efficiency. Subsequently, secondary treatment involving the cultivation of Chlorella sp. on OMWWs pretreated with chitosan achieved the highest maximal specific growth rate (0.513 ± 0.022 day⁻1) and biomass productivity (0.621 ± 0.021 g/L/day). Notably, the fatty acids (FA) profile produced by Chlorella sp. cells grown under these conditions differed, underscoring the potential of OMWWs as a microalgal growth medium. This innovative approach not only addresses environmental issues but also opens new avenues for sustainable bioproducts.","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142365209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0