International Microbiology最新文献

Dysbiosis of the rice leaf phyllomicrobiome induced by Magnaporthe oryzae infection: evidence from metabarcoding and microbiome imprinting. 稻瘟病菌侵染引起水稻叶根微生物群失调：来自元条形码和微生物群印迹的证据。

IF 2.3 4区生物学

International Microbiology Pub Date : 2025-07-23 DOI: 10.1007/s10123-025-00691-2

Charishma Krishnappa, Kuleshwar Prasad Sahu, Mushineni Ashajyothi, Mukesh Kumar, Bhaskar Reddy, Aundy Kumar

{"title":"Dysbiosis of the rice leaf phyllomicrobiome induced by Magnaporthe oryzae infection: evidence from metabarcoding and microbiome imprinting.","authors":"Charishma Krishnappa, Kuleshwar Prasad Sahu, Mushineni Ashajyothi, Mukesh Kumar, Bhaskar Reddy, Aundy Kumar","doi":"10.1007/s10123-025-00691-2","DOIUrl":"https://doi.org/10.1007/s10123-025-00691-2","url":null,"abstract":"Rice blast, caused by Magnaporthe oryzae, remains a major constraint to global rice production, typically presenting as necrotic lesions on infected leaves. To investigate the bacterial communities associated with these lesions, we employed a novel \"Microbiome Imprinting-Metabarcoding\" approach, which generated comprehensive microbial datasets (203.34 Mb) from two blast-infected rice cultivars, aromatic Pusa Basmati 1 (PB1) and non-aromatic VL Dhan 85. Metabarcoding analysis revealed the consistent presence of several dominant bacterial genera, including Pantoea, Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium, Pseudomonas, and Chryseobacterium, across both cultivars. Notably, bacterial diversity was reduced in blast lesions compared to healthy phylloplane tissues. Lesion samples comprised 28 genera (Shannon Diversity Index: 1.66; Chao1 richness: 326.86), whereas healthy leaves harbored 48 genera (Shannon Diversity Index: 1.98; Chao1 richness: 361.82). Linear discriminant effect size (LEfSe) analysis identified specific genera such as Bifidobacterium, Desemzia, Acidovorax, and Mucilaginibacter that were uniquely associated with the dysbiotic microbial communities in infected tissues. Core microbiome analysis further revealed ten genera shared between both cultivars, with Pantoea and Allorhizobium emerging as the most abundant. These findings offer new insights into the composition and dynamics of lesion-associated bacterial communities in rice blast and highlight potential microbial targets for the development of improved disease management strategies.","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144698507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Geographic variation in the microbiome of Varroa destructor in the neighbouring countries Slovakia and Czechia. 邻国斯洛伐克和捷克的瓦罗亚破坏者微生物组的地理差异。

IF 2.3 4区生物学

International Microbiology Pub Date : 2025-07-21 DOI: 10.1007/s10123-025-00699-8

Štefánia Skičková, Miroslav Baňas, Lianet Abuin-Denis, Karolína Svobodová, Apolline Maitre, Alejandra Wu-Chuang, Dasiel Obregón, Lourdes Mateos-Hernández, Igor Majláth, Viktória Majláthová, Alena Krejčí, Alejandro Cabezas-Cruz

{"title":"Geographic variation in the microbiome of Varroa destructor in the neighbouring countries Slovakia and Czechia.","authors":"Štefánia Skičková, Miroslav Baňas, Lianet Abuin-Denis, Karolína Svobodová, Apolline Maitre, Alejandra Wu-Chuang, Dasiel Obregón, Lourdes Mateos-Hernández, Igor Majláth, Viktória Majláthová, Alena Krejčí, Alejandro Cabezas-Cruz","doi":"10.1007/s10123-025-00699-8","DOIUrl":"https://doi.org/10.1007/s10123-025-00699-8","url":null,"abstract":"Varroa destructor mite is a major threat to honeybee (Apis mellifera) populations, contributing to colony losses through parasitism and pathogen transmission. While extensive research has focused on Varroa biology and its role as a virus vector, its microbiome remains poorly understood, particularly regarding geographic variation. Here, we investigated the microbial diversity, composition, and functional potential of Varroa mite microbiota collected from two neighboring countries, Czechia and Slovakia. Using high-throughput sequencing and network analysis, we assessed alpha and beta diversity metrics, microbial co-occurrence patterns, and predicted metabolic functions. Our results revealed significant differences in microbial diversity between the two regions, with some bacterial taxa appearing more prevalent in specific populations. Network analysis suggested potential variation in the structural stability of microbial communities in Varroa mites, raising the possibility that geographic factors may influence microbial interactions. Functional profiling indicated region-associated differences in predicted metabolic pathways, possibly linked to certain bacterial taxa. While these findings provide new insights into the Varroa microbiome and its potential ecological role, the interpretation of geographic influence remains a subject of ongoing investigation to better understand its scope and underlying mechanisms. A deeper understanding of these microbial dynamics may contribute to the development of novel strategies for Varroa mite management and the conservation of honeybee health.","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144674789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First report on Fusarium oxysporum, an endophyte of Polygala sinaicum: isolation and identification of biologically active natural metabolites. 中华蓼内生真菌尖孢镰刀菌的分离与活性天然代谢产物的鉴定。

IF 2.3 4区生物学

International Microbiology Pub Date : 2025-07-14 DOI: 10.1007/s10123-025-00690-3

Elham H Amr, Noha M Sorour, Ashraf S A El-Sayed, Marwa A Fayed, Ashraf F El-Baz

{"title":"First report on Fusarium oxysporum, an endophyte of Polygala sinaicum: isolation and identification of biologically active natural metabolites.","authors":"Elham H Amr, Noha M Sorour, Ashraf S A El-Sayed, Marwa A Fayed, Ashraf F El-Baz","doi":"10.1007/s10123-025-00690-3","DOIUrl":"https://doi.org/10.1007/s10123-025-00690-3","url":null,"abstract":"Fungal endophytes inhabiting the medicinal plants have been considered repertoire for bioactive metabolites. In the current study, the medicinal plant Polygala sinaica was used for the first time as a source for endophytic fungi, which were screened for novel bioactive compounds. The potent biologically active fungal isolate was morphologically identified and molecularly verified using 18S rDNA sequencing as F. oxysporum with accession # OR616565. Two compounds were isolated using flash chromatography, identified using GC/MS and NMR techniques, and quantified using HPLC. Identified compounds were bis-(2-ethylhexyl) phthalate (DEHP) (1) and dibutyl phthalate (DBP) (2) isolated for the first time from F. oxysporum. The ethyl acetate extract of F. oxysporum exhibited potent activity against different multi-drug resistant Gram-negative and Gram-positive bacteria and Candida tropicalis. The production of DEHP was studied in different modified Wickerham media, using oat flakes, rice, and tomato as carbon sources, while corn steep liquor (CSL) and soy protein were used as nitrogen sources. CSL-containing medium exhibited the highest DEHP production by F. oxysporum at an initial pH of 7.2, 0.1% inoculum size after 15 days of incubation under static conditions at 28 °C. The biosynthesis of DEHP by F. oxysporum would serve as an excellent safe and eco-friendly source for its production to be used medicinally and industrially on a large scale with less toxic effects. The current data brings insights into the potency of Fusarium oxysporum, an endophyte of Polygala sinaica, for the production of bis-(2-ethylhexyl) phthalate (DEHP) and dibutyl phthalate (DBP).","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144626300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioelectrochemical application of an F. uniseptata pigment in a microbial fuel cell for electricity generation. 一种单胞菌色素在微生物燃料电池发电中的生物电化学应用。

IF 2.3 4区生物学

International Microbiology Pub Date : 2025-07-10 DOI: 10.1007/s10123-025-00694-z

Jesús Alberto Pérez-García, Yolanda Reyes-Vidal, Arnold Hernández-Palomares, Jesús Roberto Castán-Sánchez, Francisco Javier Bacame-Valenzuela

{"title":"Bioelectrochemical application of an F. uniseptata pigment in a microbial fuel cell for electricity generation.","authors":"Jesús Alberto Pérez-García, Yolanda Reyes-Vidal, Arnold Hernández-Palomares, Jesús Roberto Castán-Sánchez, Francisco Javier Bacame-Valenzuela","doi":"10.1007/s10123-025-00694-z","DOIUrl":"https://doi.org/10.1007/s10123-025-00694-z","url":null,"abstract":"In this work, the isolation and identification of pigment-producing fungi from substrate samples collected in the Sonoran Desert, Mexico, are described. Three fungal isolates, named CR2, SM1, and GBS, were selected for their ability to produce colored pigments. The redox properties of these pigments were characterized using UV-Vis spectroscopy and cyclic voltammetry. The GBS pigment, produced by the fungus Forliomyces uniseptata, exhibited the best electrochemical behavior, with a reversible redox cycle, indicating its potential as a redox mediator (RM) for microbial fuel cells (MFCs). The effect of different light wavelengths on the growth kinetics of F. uniseptata and pigment production was evaluated. Blue light moderately accelerated pigment biosynthesis, while darkness promoted fungal growth. Finally, the GBS pigment was tested as a RM in a MFC inoculated with Bacillus subtilis. A maximum power density of 37 μW/cm2. It is suggested that mass transfer could limit performance.","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144600359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Increased pathogens and reduced intestinal immune genes associated with fish diseases during the winter-spring transition. 在冬春过渡期间，与鱼类疾病相关的病原体增加，肠道免疫基因减少。

IF 2.3 4区生物学

International Microbiology Pub Date : 2025-07-07 DOI: 10.1007/s10123-025-00693-0

Rui Yuan, Xingkun Jin, Xiaohan Liu, Kai Hao, Yan Shi, Zhen Yang, Li Zhang, Jing Chen, Chuang Guo, Yafeng Wu, Jiajin Tang, Zhe Zhao

{"title":"Increased pathogens and reduced intestinal immune genes associated with fish diseases during the winter-spring transition.","authors":"Rui Yuan, Xingkun Jin, Xiaohan Liu, Kai Hao, Yan Shi, Zhen Yang, Li Zhang, Jing Chen, Chuang Guo, Yafeng Wu, Jiajin Tang, Zhe Zhao","doi":"10.1007/s10123-025-00693-0","DOIUrl":"https://doi.org/10.1007/s10123-025-00693-0","url":null,"abstract":"Global climate change has posed significant challenges to the aquaculture industry, leading to the emergence of novel diseases. A recently emerging disease, termed the overwintering syndrome, has been reported in freshwater fish aquaculture systems in China during the winter-spring transition. This syndrome is characterized by weight loss, decreased activity levels, and mass mortality. Currently, little is known about cause of the emerging disease. In this study, the gut microbiota of healthy and diseased channel catfish (Ictalurus punctatus) were analyzed using metagenomic sequencing. Diseased fish exhibited a notable reduction in bacterial diversity compared to healthy controls, while the viral richness was significantly higher. Additionally, random forest models based on gut microbiota composition revealed high accuracy in distinguishing between diseased and healthy fish. Several gut microbiota biomarkers with potential diagnostic value were identified, primarily consisting of rare taxa (relative abundance < 5%), except for Alphaproteobacteira, Siphoviridae and Podoviridae. Furthermore, an increase in the prevalence and abundance of pathogens was observed in the intestines of diseased fish compared to healthy counterparts. Notably, Vibrio cholerae and Dickeya dadantii were enriched in the intestines of fish exhibiting the overwintering syndrome. Concurrently, genes involved in the synthesis of innate immune factors was markedly diminished in the gut microbiota of diseased fish, along with a reduction in the microbial hosts of these genes. Collectively, these findings elucidate the enrichment of pathogens and the attenuation of innate immunity in fish with the overwintering syndrome, correlating with alterations in the gut microbiota.","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144575457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spore production optimization of a biofertilizer based on Bacillus cabrialesii HB7 for enhancing plant growth under saline stress. 基于cabrialesii HB7的生物肥料促进盐胁迫下植物生长的产孢优化

IF 2.3 4区生物学

International Microbiology Pub Date : 2025-07-07 DOI: 10.1007/s10123-025-00692-1

Sahar Keskes, Saoussen Ben Khedher, Fatma Masmoudi, Imen Saadaoui, Slim Tounsi

{"title":"Spore production optimization of a biofertilizer based on Bacillus cabrialesii HB7 for enhancing plant growth under saline stress.","authors":"Sahar Keskes, Saoussen Ben Khedher, Fatma Masmoudi, Imen Saadaoui, Slim Tounsi","doi":"10.1007/s10123-025-00692-1","DOIUrl":"https://doi.org/10.1007/s10123-025-00692-1","url":null,"abstract":"Bacillus cabrialesii HB7, a novel halotolerant strain isolated from Qatari soil, can effectively promote tomato plant growth and mitigate saline stress and has a great biofertilizer potential. Due to its promising agronomical and biotechnological potential, high spore densities should be achieved, to extend its use. In this study, culture conditions were optimized with different methods to improve HB7 spore production. Firstly, three culture media were tested to find the best medium for high spore density. When using the semisynthetic medium supplemented with 15 g.L-1 NaCl, high spore production was achieved compared to complex medium and G10 media with and without NaCl addition. Secondly, key fermentation parameters were determined by Plackett-Burman design, and the optimum levels of six components (yeast extract, KH2PO4, casein hydrolysate, (NH4)2SO4, NaCl concentrations, and pH) were optimized with a hybrid design, achieving 1.82 × 109 spore.mL-1 and a threefold spore production improvement, when compared to the basal medium. Moreover, HB7 exhibited faster cell growth (0.97 h-1 vs. 0.53 h-1) and glucose uptake (1.09 h-1 vs. 0.89 h-1) after optimization. These findings may play a pivotal role in the synthesis of an effective biofertilizer based on B. cabrialesii HB7 for sustainable agriculture and biotechnological advancements.","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144575458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ilimaquinone as a novel marine sponge-derived antibacterial agent: mechanistic insights into its antibiofilm and quorum sensing inhibitory properties targeting bacterial virulence. Ilimaquinone作为一种新型海洋海绵来源的抗菌剂：其抗生素膜和群体感应抑制细菌毒力的机制见解。

IF 2.3 4区生物学

International Microbiology Pub Date : 2025-07-02 DOI: 10.1007/s10123-025-00689-w

Malvi Surti, Mitesh Patel, Reem Binsuwaidan, Mohd Adnan, Nawaf Alshammari, Syeda Bushra Fatima, Mandadi Narsimha Reddy

{"title":"Ilimaquinone as a novel marine sponge-derived antibacterial agent: mechanistic insights into its antibiofilm and quorum sensing inhibitory properties targeting bacterial virulence.","authors":"Malvi Surti, Mitesh Patel, Reem Binsuwaidan, Mohd Adnan, Nawaf Alshammari, Syeda Bushra Fatima, Mandadi Narsimha Reddy","doi":"10.1007/s10123-025-00689-w","DOIUrl":"https://doi.org/10.1007/s10123-025-00689-w","url":null,"abstract":"This study evaluates the antibacterial, antibiofilm, and anti-quorum-sensing (QS) properties of ilimaquinone (IQ) against Gram-positive and Gram-negative pathogens. The agar cup diffusion method revealed significant bacterial inhibition, with minimum inhibitory (MIC) and bactericidal (MBC) concentrations ranging from 6.25 to 25 μM and 12.5 to 50 μM, respectively. IQ exhibited dose-dependent biofilm inhibition, demonstrating its potential as an anti-biofilm agent. QS inhibition was assessed by pigment suppression in Chromobacterium violaceum (violacein), Serratia marcescens (prodigiosin), and Pseudomonas aeruginosa (pyocyanin, pyoverdine), reducing their production by 73.33%, 53.68%, 57.13%, and 62.42%, respectively, at sub-MIC concentrations. IQ also inhibited QS-regulated virulence factors in P. aeruginosa, including LasA protease, elastase, rhamnolipid, and extracellular polymeric substance (EPS) production, disrupting biofilm formation. Molecular docking and dynamics analysis confirmed strong binding affinities of IQ to key QS and biofilm-associated proteins (EsaI, PilY1, LasA, PilT, LasR, RhlR, LasI, PqsR, CviR, and CviR'), highlighting its mechanistic role in QS inhibition. These findings suggest that IQ is a promising antibacterial and anti-QS compound with potential therapeutic applications for managing bacterial infections and biofilm-related complications.","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144540190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: Population dynamics of a bacterial consortium from a marine sediment of the Gulf of Mexico during biodegradation of the aromatic fraction of heavy crude oil. 修正：墨西哥湾海洋沉积物中重质原油芳烃组分生物降解过程中细菌联合体的种群动态。

IF 2.3 4区生物学

International Microbiology Pub Date : 2025-07-01 DOI: 10.1007/s10123-025-00684-1

Maria-Pilar Muñoz-Rivera, Fernando Martínez-Morales, Daniel Morales-Guzmán, Abraham Rivera-Ramírez, Ayixon Sánchez-Reyes, María R Trejo-Hernández

引用次数: 0

Investigating the novel Escherichia coli bacteriophage Jgk1 as a potential antimicrobial agent. 研究新型大肠杆菌噬菌体Jgk1作为潜在的抗菌剂。

IF 2.3 4区生物学

International Microbiology Pub Date : 2025-06-20 DOI: 10.1007/s10123-025-00687-y

Mengxin Gong, Mingyuan Li, Jilian Wang, Jiaxuan Liu, Qing Meng

{"title":"Investigating the novel Escherichia coli bacteriophage Jgk1 as a potential antimicrobial agent.","authors":"Mengxin Gong, Mingyuan Li, Jilian Wang, Jiaxuan Liu, Qing Meng","doi":"10.1007/s10123-025-00687-y","DOIUrl":"10.1007/s10123-025-00687-y","url":null,"abstract":"Enterobacteriaceae are significant opportunistic pathogens in the environment, impacting both biological and human health. The rise in antibiotic-resistant strains has compelled the exploration of bacteriophages as alternative biological control agents. In this study, we isolated a bacteriophage JgK1 specific to Escherichia coli from environmental samples. This bacteriophage can effectively lyse Escherichia coli jg1, exhibiting an optimal MOI of 0.01 and an average mutation rate of 1.96 × 10-7. One-step growth curve analysis revealed an incubation period of 25 min and a release amount of approximately 430.75 pfu/infection center. It can withstand high temperatures of 55 °C. Additionally, whole genome sequencing (WGS) confirmed a genome size of 74,059 bp, containing 107 open reading frames (ORF), with no virulence or resistance-related genes, nor lysogenic genes. Comparative genomic analysis indicated lower coverage compared to other known isolated bacteriophages, with the highest coverage being 72%. This study suggests that bacteriophage JgK1 has the potential to serve as an effective biological control antibacterial agent, offering a promising alternative to antibiotics for managing Escherichia coli infections.","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144333112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacterial community composition and diversity associated with developmental stages of Anopheles subpictus. 亚按蚊细菌群落组成和多样性与发育阶段的关系。

IF 2.3 4区生物学

International Microbiology Pub Date : 2025-06-20 DOI: 10.1007/s10123-025-00688-x

Ankita Agrawal, Aishwarya B Acharya, Bijayalaxmi Sahu, Tapan Kumar Barik, Amiya Kumar Patel

{"title":"Bacterial community composition and diversity associated with developmental stages of Anopheles subpictus.","authors":"Ankita Agrawal, Aishwarya B Acharya, Bijayalaxmi Sahu, Tapan Kumar Barik, Amiya Kumar Patel","doi":"10.1007/s10123-025-00688-x","DOIUrl":"10.1007/s10123-025-00688-x","url":null,"abstract":"Malaria remains the deadliest vector-borne disease globally. Among different malaria vectors, Anopheles subpictus has been regarded as the potent vector across many Indian states. Microbiota in mosquitoes are critical determinants for their development, physiology, and vector competence, which differ significantly among mosquito species, life stages, and environmental parameters. Microbial profiles linked with various life stages of the emerging malaria vector, An. subpictus remain unexplored. In this study, 16S rRNA amplicon sequencing (V1-V9 regions) was used to explore and analyze the microbial community dynamics within and between life stages. A total of 287,077 bacterial reads were generated and distributed into 40 phyla, 75 classes, 160 orders, 334 families, 961 genera, and 2398 species. Diversity analyses revealed that bacterial community structure varied significantly across the three life phases (larvae, pupae, adult) of An. subpictus. The larval stage has higher species richness compared to subsequent developmental stages. The sequence-based taxonomic profiling revealed that Methylobacterium, Clostridium, Bradyrhizobium, Cytophaga, Roseateles, Mitsuaria, Sphingomonas and Wolbachia were the dominant bacterial genera across the different life stages. Moreover, Methylobacterium sp. (51.6%) was the most abundant taxa in the adult female stage, whereas the larval stage was mainly dominated by Cytophaga (15.2%). This research advances our understanding of bacterial community composition and dynamics across the developmental succession of An. subpictus. The finding pinpoints dominant bacterial candidates that could be harnessed to develop microbial-based control strategies aimed at limiting mosquito-borne diseases.","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144333110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0