International Microbiology最新文献

{"title":"Dickeya zeae WH1 as sensor for cost-effective detection of pyocyanin produced by Pseudomonas aeruginosa.","authors":"Xiaojuan Tan, Guangyu Ju, Dandan Feng, Liuji Chen, Xiaodie Fan, Cancan Zhu, Minghui Li, Dongsheng Du","doi":"10.1007/s10123-025-00676-1","DOIUrl":"https://doi.org/10.1007/s10123-025-00676-1","url":null,"abstract":"<p><p>Pyocyanin is a crucial virulence factor uniquely secreted by Pseudomonas aeruginosa. Hence, the rapid and selective detection of pyocyanin can reveal essential information about the pathogenesis of diseases caused by clinical isolates. Herein, we found that P. aeruginosa PAO1 exhibited remarkable antagonistic activity against Dickeya zeae WH1. Subsequently, its active compounds were purified by preparative high-performance liquid chromatography and HPLC, and anti-WH1 activity was determined by performing the minimum inhibitory concentration (MIC) assay. Only one active compound, pyocyanin, was obtained. Importantly, the linear quantification of pyocyanin to the biomass (OD600) of WH1 showed an R² value of 0.9908 ranging from 0.195 to 6.25 µg/mL, and pyocyanin concentration was accurately determined using this curve when P. aeruginosa was cultured in high-, medium-, and low-pyocyanin-producing media. Therefore, using D. zeae WH1 as a sensor for detecting pyocyanin and high-pyocyanin-producing isolates by high-throughput screening is a cost-effective and simple method.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144181593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Challenges and distortions in microbial community analysis of oil reservoirs: a case study with heavy crude oil from the Romashkino field.","authors":"Liliya Biktasheva, Polina Galitskaya, Polina Kuryntseva, Maria Shipaeva, Svetlana Selivanovskaya","doi":"10.1007/s10123-025-00675-2","DOIUrl":"https://doi.org/10.1007/s10123-025-00675-2","url":null,"abstract":"<p><p>The study of the microbial community of wells is a methodologically complex, but urgent problem. In the course of our work, five samples of oil wells were selected from one deposit of the Romashkino field. The samples were subjected to nucleic acid extraction using three methods-direct DNA extraction, and after enrichment using aerobic and anaerobic cultivation methods. In three samples from wells W1-W3, extraction after anaerobic enrichment was successful. Effective aerobic cultivation was possible in all five samples. All three of these samples represented the aqueous part of the produced fluid; samples from wells W4 and W5, where extraction was difficult, represented the oil part. During the analysis of the microbial community in enrichment cultures from wells W1-W3, exogenous microorganisms such as Desulfovibrio, Acetobacterium, Bacillus, and Georgenia were discovered, which can be explained by the long-term exploitation of this section of the field. In one sample from well W1, community information was obtained using direct extraction and anaerobic enrichment. It was found that the microbial community changed significantly after enrichment, and its diversity decreased. At the same time, however, the functional profile of microorganisms has not changed, and sulfate-reducing microorganisms dominate in both samples. Thus, the results of the work allow us to make an assumption about the physicochemical parameters of samples in which the study of the microbial community is possible. In addition, it became known that well W1 needs to control the process of biological acidification and has a high risk of equipment corrosion.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144172868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the effects of different formulations of protectants on the preservation of the microbiota in fecal microbiota transplantation.","authors":"Liyu Chen, Chong Chen, Yang Bai, Cailan Li, Chongai Wei, Riqing Wei, Rongrong Luo, Ru Li, Qiang Ma, Yan Geng","doi":"10.1007/s10123-025-00663-6","DOIUrl":"https://doi.org/10.1007/s10123-025-00663-6","url":null,"abstract":"<p><strong>Background: </strong>With the increasing indications for fecal microbiota transplantation for the treatment of diseases, there is a growing demand for the preparation of frozen or lyophilized fecal microbiota products that are viable and can stably colonize the recipient. The addition of protective agents plays an important role in the preparation. However, there has been no systematic evaluation of the protective agents used in fecal microbiota sample transplantation preparation for transplantation.</p><p><strong>Methods: </strong>We were used the donor bacterial flora containing 10 different formulations of protective agents were frozen, lyophilized, and stored. Plate counting, CCK8 assay, flow cytometry after LIVE/DEAD staining, and fluorescence intensity were used to assess viable bacteria in vitro. In addition, the donor bacterial flora samples containing different formulations protective agents were transplanted into antibiotic-treated SPF mice, with 3 mice in each group and a total of 5 groups. Fecal samples were collected for metagenomic sequencing to observe the colonization of the bacterial flora in the recipient mice.</p><p><strong>Results: </strong>The preliminary screening results showed that the survival rate of bacteria in the 5% trehalose (T) groups, and 5% sucrose, 5% inulin, and 1% cysteine hydrochloride (SI) groups was slightly higher than that in the other groups. SI groups tended to be more protective against anaerobes than T groups. The donor gut microbiota containing the SI groups protective agent exhibited the best colonization of the recipient mice. The protective effects of different formulations of protective agents on the colonized probiotic strains and the metabolic function of the bacterial flora in recipient mice were found to be species specific.</p><p><strong>Conclusions: </strong>SI groups can not only better protect the activity of anaerobic bacteria in the intestine, but also effectively promote the effective colonization of donor intestinal bacteria in the recipient mice, and the effect of frozen storage method is less, and can be used at the same time as frozen and freeze-dried preparation. It can be used as a reference for the selection of protective agents in the preparation of fecal microbiota transplantation samples.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144136224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic properties of mercury‑tolerant yeast, Pichia kudriavzevii 1P4.","authors":"Rika Indri Astuti, Indra Maulana, Wulan Tri Wahyuni, Anja Meryandini","doi":"10.1007/s10123-025-00666-3","DOIUrl":"https://doi.org/10.1007/s10123-025-00666-3","url":null,"abstract":"<p><p>Mercury contamination in commercial products poses serious risks to human health and the environment. The in vitro detection methods using spectroscopy approaches are expensive and have limited practical use. Yeast-based biosensors provide a more affordable and user-friendly alternative. Therefore, the exploration of mercury tolerance yeast is essential to support the sensor properties. In this study, we investigated the mercury tolerance of 10 yeast isolates. Pichia kudriavzevii 1P4 exhibited tolerance up to 1.5 mM HgCl₂ while showing slow growth phenotype as grown in 2 mM HgCl₂. This is the first report to show the ability of this genus of the yeast Pichia to cope with HgCl₂ stress. X-ray spectra showed Hg accumulation in yeast colonies grown in HgCl2, while none was detected in colonies grown without it. This data indicates the capability of 1P4 in the accumulation of Hg as one of the HgCl₂-stress tolerance mechanisms. Whole-genome sequencing of isolate 1P4, using the MGI DNBSEQ-G400 platform, revealed a genome size of 10.8 Mbp across five chromosomes, with a GC content of 38.97%. The largest portions of the genome are involved in translation, amino acid transport, metabolism, and protein modification, based on COG analysis. Key genes potentially contributing to mercury tolerance involve efflux/inorganic ion transport system (e.g., FieF, Acr3/B, and CzcO/D-clusters) and glutathione-associated oxidative stress response (e.g., BtuE and LysX, which encoded glutathione peroxidase and glutathione synthase). Those genes were predominant in COG category of inorganic ion transport and metabolism (P) and defense mechanisms (V). The genome of 1P4 was assembled into 4753 gene clusters, where 2222 of which were shared with the other species of yeast, including Pichia membranifaciens, P. kluyveri, P. inconspicua, S. cerevisiae, and C. albicans. Meanwhile, 921 gene clusters were shared among genera of Pichia spp., only. These findings highlight the genetic profile and mercury-tolerance mechanisms of isolate 1P4, supporting its potential application as a mercury biosensor or bioremediation agent.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144132391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocontrol potential of bacteria associated with Asian walnut moth Erschoviella musculana Erschoff (Lepidoptera: Nolidae) on walnut pests.","authors":"Hande Bayraktar Şalvarci, Donus Gencer, Ardahan Eski, Vildan Bozkurt, İsmail Demir","doi":"10.1007/s10123-025-00674-3","DOIUrl":"https://doi.org/10.1007/s10123-025-00674-3","url":null,"abstract":"<p><p>The aim of this study was to investigate the culturable bacterial flora of Erschoviella musculana, which causes damage to leaves, shoots, branches, trunk, and fruits of walnut trees, in order to find a possible microbial control agent against walnut pests. In this study, a total of 11 bacteria were isolated from live and dead larvae of E. musculana and identified based on their morphological, biochemical, and molecular characteristics. Based on these results, the isolates were identified as Bacillus toyonensis EMB1, Bacillus toyonensis EMB3, Stenotrophomonas rhizophila EMO6, Stenotrophomonas rhizophila EM8, Delftia tsuruhatensis EM14, Acinetobacter modestus EM20, Lelliottia amnigena EM21, Bacillus toyonensis EM22, Pantoea vagans EM37, Pseudomonas alloputida EM42, and Stenotrophomonas geniculata EM43. The biological control potential of these isolates on E. musculana, Hyphantria cunea (Lepidoptera: Arctiidae), and Panaphis juglandis (Hemiptera: Callaphididae), which are very important walnut pests, was determined. The results showed that B. toyonensis strain EMB1 had the highest virulence (82%) on the larvae of H. cunea and S. rhizophila strain EM8 had the highest virulence (83%) on the nymphs of P. juglandis. On the other hand, S. geniculata strain EM43 caused 100% mortality on the larvae of E. musculana. The results of this study show that bacteria associated with E. musculana appear to be promising microbial control agents for walnut pests.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144077988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening, isolation, and process optimization of a bioemulsifier-producing Acinetobacter Y-1.","authors":"Hui Wei, Fu-Rong Yao, Ou-Ying Li, Shuo Guo, Jun Li, He-Jing Yan, Feng-Mei Zhu, Jie-Fang Zhou","doi":"10.1007/s10123-025-00664-5","DOIUrl":"https://doi.org/10.1007/s10123-025-00664-5","url":null,"abstract":"<p><p>Optimizing the production of bioemulsifiers is critical for sustainable industrial applications. In this study, 27 strains were isolated from activated sludge, and strain Y-1 was identified as the most promising candidate based on emulsification index (EI) and emulsification rating. Strain Y-1 exhibited excellent emulsifying and dispersing activities toward both liquid paraffin and crude oil, with an EI of 65%. Morphological characterization, physiological and biochemical tests, and 16S rDNA sequencing identified Y-1 as a member of the genus Acinetobacter. Fermentation conditions were optimized using single-factor experiments and response surface methodology, and the optimal conditions were determined to be a carbon-to-nitrogen (C/N) ratio of 1:1, inoculum concentration of 2%, and incubation temperature of 34 °C. Under these conditions, the bioemulsifier yield of Acinetobacter Y-1 reached 6.9 g/L, representing a 4.14-fold increase compared to unoptimized conditions. The bioemulsifier produced by Acinetobacter Y-1 effectively emulsified a variety of hydrocarbon substrates, including liquid paraffin, diesel, xylene, olive oil, cutting oil, cold rolling oil, n-hexane, and benzene. Furthermore, it retained stable emulsification activity under extreme conditions, including a wide temperature range (4-80 °C), high salinity (5-30%), and broad pH values (1-14). These findings highlight Acinetobacter Y-1 as a promising strain for the production of high-activity, stable bioemulsifiers with great potential for industrial applications.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Borrelidin M: a new borrelidin derivative obtained from Streptomyces rochei VL-16 exhibited potent antibacterial and antiproliferative properties.","authors":"Vinoth Vengadesan, Jagan Muniyandi, Neha Yadav, Premkumar G, Ajit Ramesh Sawant, Lakshmi Sankaranarayanan, Prashanth K, Toka Swu, Sudha Rani Sadras","doi":"10.1007/s10123-025-00673-4","DOIUrl":"https://doi.org/10.1007/s10123-025-00673-4","url":null,"abstract":"<p><p>The lookout for novel metabolites to overcome the threat imposed by antibiotic resistant pathogens is on the rise globally. The present study reports on the identification and characterisation of a new borrelidin derivative from Streptomyces rochei VL-16 strain that was isolated from the soil samples of Osudu Lake in Puducherry. The active compound produced by Streptomyces rochei VL-16 strain was purified sequentially by thin layer, column and high performance liquid chromatography techniques. The purified compound was subsequently identified as borrelidin through Q-TOF-HR-LCMS, FTIR and UV-Vis spectroscopy techniques. Based on integration proton NMR signals and HR-ESI-MS peak pattern, the compound was further identified as a new derivative of borrelidin. Its 3D structure was optimised using density functional theory. The new derivative obtained in this study was designated as borrelidin M (C₂₈H₄₁NO_5, Mol. wt.471.3 g/mole). This derivative was found to exhibit potent inhibitory effects against the foodborne pathogens including Clostridium perfringens, Aeromonas hydrophila, Staphylococcus aureus, Listeria monocytogenes and Yersinia enterocolitica. Also, borrelidin M exhibited anticancer and anti-metastatic effects against A549 cell line with an IC₅₀ value of 17.5 μM. Collectively, these findings indicated promising biomedical applications of this new derivative of borrelidin.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144077994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of water-saving irrigation on microbial community structures, assembly, and metabolic activities in alfalfa rhizosphere soils.","authors":"Feng Ding, Shun Ji, Renna Sa, Chenbo Nin, Feng Ma, Haijun Yan","doi":"10.1007/s10123-025-00667-2","DOIUrl":"https://doi.org/10.1007/s10123-025-00667-2","url":null,"abstract":"<p><p>In agricultural areas of arid Xinjiang, China, selecting appropriate irrigation strategies for farmland is essential. Increasing attention is being paid to the ecological effects of different irrigation methods on the soil environment. As a crucial component of soil quality, the microbial community is a key indicator of the impacts of irrigation on the soil environment. To investigate the effects of irrigation treatments on the properties of rhizosphere soil and the underlying microbial community characteristics, this study conducted an alfalfa field experiment applying three water-saving treatments (3750, 4500, and 5250 m³ hm^-2) and regular irrigation (6750 m³ hm^-2, CK). The results showed that the water-saving treatments increased the soil pH, salinity, available nitrogen, and phosphorus levels. The water-saving treatments decreased the richness and diversity of the bacterial community in the rhizosphere but increased those of the fungal community. The influence of stochastic processes on fungal and bacterial communities assembly under water-saving treatments was more noticeable than that under CK. Compared with CK, water-saving treatments reduced the complexity of microbial network and increased the potential negative interaction between bacteria and fungi. Functional prediction analysis showed that species specificity among treatments may result from a specific selection of rhizosphere functional requirements. This study reveals the effect of controlling irrigation quantity on protecting soil microbial diversity and function and improves the understanding of rhizosphere soil community response affected by different irrigation strategies. The results facilitate the development of effective and beneficial agricultural measures.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144010669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of trace metal composition utilizing Taguchi orthogonal array enhances biomass and superoxide dismutase production in Tetraselmis chuii under mixotrophic condition: implications for antioxidant formulations.","authors":"Chiu Peng Chan, Huang Zhen Ni, Sangkaran Pannerchelvan, Murni Halim, Joo Shun Tan, Nor Azman Kasan, Mohd Shamzi Mohamed","doi":"10.1007/s10123-025-00672-5","DOIUrl":"https://doi.org/10.1007/s10123-025-00672-5","url":null,"abstract":"<p><p>The natural ageing process in all organisms is majorly influenced by the production rate and dismutation of reactive oxygen species (ROS) within cells. Certain microalgae, such as Tetraselmis chuii, possess the ability to produce superoxide dismutase (SOD), a powerful antioxidant enzyme that mitigates oxidative damage caused by ROS during oxygen metabolism. This study investigated the impact of trace elements (nickel, manganese, copper, zinc, and iron) and nitrogen sources in the growth medium on both the biomass and SOD synthesis of T. chuii under mixotrophic conditions. Initially, the one-factor-at-a-time (OFAT) approach was employed to filter out the most significant factors in the production medium. Next, Taguchi orthogonal array method, known for its robustness in experimental design, was employed to analyse the effects of various media components on algal biomass and SOD production. Using only a few well-defined experimental sets, Taguchi's L18 orthogonal array facilitated a 1.21-fold increase in biomass yield, reaching a maximum of 0.643 g/L. Furthermore, SOD activity was enhanced from 85.28 to 91.94% following optimization. Notably, nitrogen source, nitrogen concentration, and zinc concentration emerged as significant influencers of biomass and SOD production. The Taguchi optimization thereby improved SOD yield in a cost-effective manner. The heightened antioxidation activity of SOD holds promising applications in formulating antioxidants and topical ointments in pharmaceutical and cosmeceutical industries.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144012023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial consortium involving Pseudomonas and Bacillus: strain selection and the effect of co-cultivation on biocontrol activity against phytopathogens and the composition of metabolic extracts.","authors":"João Gabriel Dumont Negrelli, Maira Rafaela de Britto Rafael, Vitor Dib Gazola, Marcus Cesar Bochi Reis Dos Santos, Eduardo Jorge Pilau, Andressa Domingos Polli, Halison Correia Golias, Tiago Tognolli de Almeida, Julio Cesar Polonio","doi":"10.1007/s10123-025-00668-1","DOIUrl":"https://doi.org/10.1007/s10123-025-00668-1","url":null,"abstract":"<p><p>Agricultural sustainability is vital to meet the growing global demand for food; therefore, the search for more sustainable options to replace traditional chemical products has gained attention due to their benefits. The sector has applied innovative microbial consortium approaches as a niche for exploring new bioproducts and metabolic pathways through microbial interactions. Thus, this study sought to select two endophytic bacterial strains with biocontrol activity to study their metabolic interactions in culture. For this, co-cultivation and axenic culture assays were carried out to evaluate the inhibition of Corynespora cassiicola, Sclerotinia sclerotiorum, Moniliophthora perniciosa, and Colletotrichum truncatum. After the production of antiphytopathogenic compound tests, two strains were selected: P. putida MG36 and B. amyloliquefaciens SS14. These bacteria were cultivated under three distinct conditions: axenic cultivation of SS14, axenic cultivation of MG36, and co-cultivation. The metabolites were extracted and analyzed by liquid chromatography coupled with mass spectrometry (UHPLC-MS/MS). The results showed that both strains exhibited significant antifungal activity. B. amyloliquefaciens SS14 demonstrated 65% inhibition against C. truncatum, while P. putida MG36 showed 58% inhibition against S. sclerotiorum. Analysis of the chemical profiles revealed the presence of exclusive and shared metabolites, such as iturin A4 (antifungal lipopeptide) and macrolactin A (bioactive polyketide), under different culture conditions. In conclusion, P. putida MG36 and B. amyloliquefaciens SS14 show promise as biocontrol agents against phytopathogens, contributing to more sustainable agricultural practices.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144018426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}