International Microbiology最新文献

{"title":"Diversity, characterization, and biotechnological potential of plant growth-promoting bacteria from Bryophyllum pinnatum (Lam.) (Crassulaceae) roots and rhizosphere soil.","authors":"Jianyun Zhang, Siyu Liao, Weiping Gao, Axing Qubie, Dongmei He, Hai Wang, Zhuyun Yan","doi":"10.1007/s10123-025-00633-y","DOIUrl":"https://doi.org/10.1007/s10123-025-00633-y","url":null,"abstract":"<p><p>Cultivable microbial communities associated with plants inhabiting extreme environments have great potential in biotechnological applications. However, there is a lack of knowledge about these microorganisms from Bryophyllum pinnatum (which survives in severely barren soil) and their ability to promote plant growth. The present study focused on the isolation, identification, biochemical characterization, and potential applications of root endophytic bacteria and rhizosphere bacteria. A total of 73 bacterial isolates were obtained, with 50 derived from rhizospheric soil and 23 from root tissue. The identified strains were categorized into 16 genera, with Bacillus, Priestia, Pseudarthrobacter, Neobacillus, Mesobacillus, and Arthrobacter being the most species-rich genera. Heat stress experiments indicated that almost half (50.7%) of the selected isolates were tolerant to heat stress. Furthermore, most strains present diverse capabilities for biotechnological applications, including the potential for indole-3-acetic acid (IAA) production, organic phosphorus solubilization, inorganic phosphorus solubilization, and nitrogen fixation. Some isolates (21.92%) exhibited broad-spectrum antagonistic activity against various phytopathogenic fungi, including Fusarium spp. Agar plate assays revealed that the Cellulomonas hominis strain LS43 and Bacillus inaquosorum strain LS77 significantly increased the total fresh weight of Arabidopsis (P < 0.05), yet these strains did not significantly affect the primary root length or the number of leaves. Notably, a subset of the strains tested did not significantly increase the growth of Arabidopsis and, in fact, had inhibitory effects on certain growth parameters. This is the first investigation highlighting the potential of root endophytic bacteria and rhizosphere bacteria in association with B. pinnatum in barren soils. Thus, these isolated strains positively influence plant nutrient uptake, stress resilience, and biocontrol to reduce chemical inputs in conventional agricultural practices, highlighting the importance of their development as biofertilizers for improving the quality of barren soil.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143005042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polysaccharide lyase PL3.3 possibly potentiating Clostridioides difficile clinical symptoms based on complete genome analysis of RT046/ST35 and RT012/ST54.","authors":"Sen Zhang, Wen Ma, Xin Zhang, Weitong Cui, Youhan Liu, Xuewen Tian, Qinglu Wang, Ying Luo","doi":"10.1007/s10123-025-00634-x","DOIUrl":"https://doi.org/10.1007/s10123-025-00634-x","url":null,"abstract":"<p><p>Clostridioides difficile has rapidly become a major cause of nosocomial infectious diarrhea worldwide due to the misuse of antibiotics. Our previous study confirmed that RT046/ST35 strain is associated with more severe clinical symptoms compared to RT012/ST54 strain. We conducted genome comparison of the RT046/ST35 and RT012/ST54 strains using whole-genome sequencing technology. The RT046/ST35 strain had a genome length of 7,869,254 bp with a GC content of 29.49%. The original length of the RT012/ST54 strain was 7,499,568 bp with a GC content of 29.64%. Additionally, we detected plasmid1 in the RT046/ST54 strain. We found that the RT046/ST35 strain had more genomic islands compared to the RT012/ST54 strain, and we identified polysaccharide lyase (PL) in the region around 2.2 M. Furthermore, we discovered that the increased severity of clinical symptoms in the RT046/ST35 strain compared to the RT012/ST54 strain was unrelated to virulence factors and emphasized the potential crucial role of PL in RT046/ST35. There were almost no differences in eggNOG annotation and KEGG annotation between RT046/ST35 and RT012/ST54. RT046/ST35 had more mRNA processes in GO annotation. In conclusion, our study suggests that the core factor contributing to the more serious clinical symptoms of the RT046/ST35 strain compared to the RT012/ST54 strain is possibly PL.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143005136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differences of microbial growth and biofilm formation among periprosthetic joint infection-causing species: an animal study.","authors":"Mehmet Batu Ertan, Mehmet Yağız Ayduğan, Ebru Evren, İrem İnanç, Esra Erdemli, Bülent Erdemli","doi":"10.1007/s10123-024-00629-0","DOIUrl":"https://doi.org/10.1007/s10123-024-00629-0","url":null,"abstract":"<p><strong>Purpose: </strong>The most frequently used surgical procedures for periprosthetic joint infections (PJIs) are debridement, antibiotics, and implant retention (DAIR), as well as single- or two-stage revision arthroplasty. The choice of surgery is made depending on the full maturation of the biofilm layer. The purpose of this study was to evaluate the biofilm formation and microbial growth using common PJI-causing agents and compare its development on the implant surface.</p><p><strong>Methods: </strong>The in vivo study was performed using 40 Sprague-Dawley rats divided into five groups (n = 8/group): Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Candida albicans, and control. Six standard titanium alloy discs were placed into the subcutaneous air pouches of the interscapular areas of the rats. After the inoculation of microorganisms, disc and soft tissue cultures were collected at 2-week intervals for 6 weeks, and the microbial load and the microscopic appearance of the biofilm were compared.</p><p><strong>Results: </strong>The disc samples from the S. aureus group had the highest infection load at all time points; however, in soft tissue samples, this was only observed at week 4 and 6. Electron microscopic images showed no distinctive differences in the biofilm structures between the groups.</p><p><strong>Conclusion: </strong>S. aureus microbial burden was significantly higher in implant cultures at week 2 compared to other PJI-causing agents examined. These results may explain the higher failure rate seen if the DAIR procedure was performed at < 3-4 weeks after the PJI symptom onset and support the observation that DAIR may not be effective against PJIs caused by S. aureus.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143005037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking olive rhizobacteria: harnessing biocontrol power to combat olive root rot and promote plant growth.","authors":"Ikram Legrifi, Jamila Al Figuigui, Haitam Lahmamsi, Mohammed Taoussi, Mohammed Radi, Zineb Belabess, Abderrahim Lazraq, Essaid Ait Barka, Rachid Lahlali","doi":"10.1007/s10123-025-00632-z","DOIUrl":"https://doi.org/10.1007/s10123-025-00632-z","url":null,"abstract":"<p><p>Olive trees are susceptible to various diseases, notably root rot caused by Pythium spp., which presents significant challenges to cultivation. Conventional chemical control methods have limitations, necessitating exploration of eco-friendly alternatives like biological control strategies. This study aims to evaluate the potential of rhizobacteria in managing Pythium schmitthenneri-induced root rot in olive trees. We screened 140 bacteria isolated from olive tree rhizospheres for antifungal activity against the pathogen in vitro. Twelve isolates exhibited promising antifungal activity, identified through 16S rDNA gene sequencing as primarily Bacillus, Pseudomonas, Stenotrophomonas, and Alcaligenes species. Particularly, Pseudomonas koreensis (A28 and A29), Pseudomonas reinekei (A16), and Bacillus halotolerans (A10) were the highest effective strains. Mechanistic investigations revealed positive protease production in all twelve isolates, with eight producing amylase and cellulase. Chitinase activity was absent, while five solubilized tricalcium phosphate. Furthermore, eight secreted hydrocyanic acid (HCN), ten synthesized indole-3-acetic acid (IAA), and nine produced siderophores. Variability existed in antimicrobial substance production, including bacillomycin (seven isolates), iturin (eleven isolates), fengycin (two isolates), and surfactin (three isolates). Plant growth-promoting rhizobacteria (PGPR) capabilities were assessed using canola (Brassica napus) seedlings, showing enhanced growth in treated seedlings compared to controls. Greenhouse experiments confirmed the biocontrol efficacy of P. koreensis A28 and Bacillus subtilis C6 against root rot disease. These findings suggest these strains could serve as promising tools for managing olive tree root rot, offering a sustainable alternative to hazardous agrochemicals.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142978450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effects of nickel tungstate nanoparticles (NiWO₄ NPs) on freshwater microalga Raphidocelis subcapitata (Chlorophyceae).","authors":"Cínthia Bruno de Abreu, Renan Castelhano Gebara, Giseli Swerts Rocha, Adrislaine da Silva Mansano, Marcelo Assis, Thalles Maranesi Pereira, Luciano Sindra Virtuoso, Ailton José Moreira, Mykaelli Andrade Santos, Maria da Graça Gama Melão, Elson Longo","doi":"10.1007/s10123-024-00628-1","DOIUrl":"https://doi.org/10.1007/s10123-024-00628-1","url":null,"abstract":"<p><p>Among the vast array of functional nanoparticles (NPs) under development, nickel tungstate (NiWO₄) has gained prominence due to its potential applications as a catalyst, sensor, and in the development of supercapacitors. Consequently, new studies on the environmental impact of this material must be conducted to establish a regulatory framework for its management. This work aims to assess the effects of NiWO₄ (NPs) on multiple endpoints (e.g., growth, photosynthetic activity, and morphological and biochemical levels) of the freshwater microalga Raphidocelis subcapitata (Chlorophyceae). Quantification data revealed that the fraction of dissolved Ni and free Ni²⁺ increased proportionally with NiWO₄ NP concentrations, although these levels remained relatively low. Biological results indicated that NiWO₄ NPs did not inhibit the growth of algal cells, except at 7.9 mg L^-1, resulting in a 9% decrease. Morphological changes were observed in cell size and complexity, accompanied by physiological alterations, such as a reduction in chlorophyll a fluorescence (FL3-H) and signs of impaired photosynthetic activity, indicated by the effective quantum yield, quenchings, and chlorophyll a (Chl a) content. Furthermore, the rapid light curves showed that the NPs in high concentrations affected microalga ability to tolerate high light intensities, as corroborated by the significant decrease in the relative electron transport rate (rETRmax) and saturation irradiance (Ek). Based on the present study results, we emphasize the importance of applying integrative approaches in ecotoxicological studies, since each endpoint evaluated showed different sensitivity.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142948926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and probiotic evaluation of Apilactobacillus kunkeei and Bombella sp. from Apis mellifera anatoliaca and Bombus terrestris.","authors":"Mehtap Usta, Kübra Zengin, Samet Okuyan, Serhat Solmaz, Remziye Nalçacıoğlu, Zihni Demirbağ","doi":"10.1007/s10123-024-00631-6","DOIUrl":"https://doi.org/10.1007/s10123-024-00631-6","url":null,"abstract":"<p><p>The Anatolian honey bee (Apis mellifera anatoliaca) and Bombus terrestris are important species in Türkiye. In this context, protecting the health of these honey bees is particularly important. Lactic acid bacteria (LAB) and acetic acid bacteria (AAB) are very important for the health of bees. This study aimed to identify and evaluate the antimicrobial and probiotic properties of Lactobacillus and Bombella bacteria isolated from A. m. anatoliaca and B. terrestris. As a result of morphological (Gram staining), physiological (catalase test), and molecular analysis (16 s rRNA) studies, the bacteria were determined to be Apilactobacillus kunkeei and Bombella sp. The shape and arrangement of the bacteria were observed by scanning electron microscopy analyses. Lactobacillus isolates were determined as Gram positive and catalase negative; Bombella isolate was Gram negative and catalase positive. Probiotic properties were determined by the resistance to acid, pepsin, pancreatin, and bile salts. Results showed that all bacteria demonstrated good survival for up to 24 h at all pH values tested. It (A. kunkeei from A. m. anatoliaca) showed antimicrobial activity against Proteus vulgaris ATCC 13315 strain. Antibiotic susceptibility tests showed that all bacteria were susceptible to nystatin. Also A. kunkeei from A. m. anatoliaca was susceptible to spectinomycin, cycloheximide, kanamycin, ciprofloxacin, and streptomycin (10 mg/ml) antibiotics. All these results suggest that A. kunkeei has potential to be used as a probiotic. It is thought that the bacteria obtained as a result of this study have probiotic properties and have the potential to be used in the natural environment by preparing preparations supported by further studies.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142927132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Higher diversity of sulfur-oxidizing bacteria based on soxB gene sequencing in surface water than in spring in Wudalianchi volcanic group, NE China.","authors":"Lirong Geng, Lei Yang, Tao Liu, Shuang Zhang, Xindi Sun, Weidong Wang, Hong Pan, Lei Yan","doi":"10.1007/s10123-024-00526-6","DOIUrl":"10.1007/s10123-024-00526-6","url":null,"abstract":"<p><strong>Introduction: </strong>Sulfur-oxidizing bacteria (SOB) play a key role in the biogeochemical cycling of sulfur.</p><p><strong>Objectives: </strong>To explore SOB diversity, distribution, and physicochemical drivers in five volcanic lakes and two springs in the Wudalianchi volcanic field, China.</p><p><strong>Methods: </strong>This study analyzed microbial communities in samples via high-throughput sequencing of the soxB gene. Physical-chemical parameters were measured, and QIIME 2 (v2019.4), R, Vsearch, MEGA7, and Mothur processed the data. Alpha diversity indices and UPGMA clustering assessed community differences, while heat maps visualized intra-sample variations. Canoco 5.0 analyzed community-environment correlations, and NMDS, Adonis, and PcoA explored sample dissimilarities and environmental factor correlations. SPSS v.18.0 tested for statistical significance.</p><p><strong>Results: </strong>The diversity of SOB in surface water was higher than in springs (more than 7.27 times). We detected SOB affiliated to β-proteobacteria (72.3 %), α-proteobacteria (22.8 %), and γ-proteobacteria (4.2 %) distributed widely in these lakes and springs. Rhodoferax and Cupriavidus were most frequent in all water samples, while Rhodoferax and Bradyrhizobium are dominant in surface waters but rare in springs. SOB genera in both habitats were positively correlated. Co-occurrence analysis identified Bradyrhizobium, Blastochloris, Methylibium, and Metyhlobacterium as potential keystone taxa. Redundancy analysis (RDA) revealed positive correlations between SOB diversity and total carbon (TC), Fe²⁺, and total nitrogen (TN) in all water samples.</p><p><strong>Conclusion: </strong>The diversity and community structure of SOB in volcanic lakes and springs in the Wudalianchi volcanic group were clarified. Moreover, the diversity and abundance of SOB decreased with the variation of water openness, from open lakes to semi-enclosed lakes and enclosed lakes.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":"119-136"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140916559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Restoration of the soil fertility under Cr(VI) and artificial drought condition by the utilization of plant growth-promoting Bacillus spp. SSAU2.","authors":"Abhijeet Sharma, Shailendra Kumar Singh, Neetu Maurya, Shashank Mani Tripathi, Saumya Jaiswal, Manshi Agrawal, Shanthy Sundaram","doi":"10.1007/s10123-024-00528-4","DOIUrl":"10.1007/s10123-024-00528-4","url":null,"abstract":"<p><p>The study explores the potential of an indigenous halo-tolerant microbe identified as Bacillus spp. SSAU-2 in enhancing soil fertility and promoting plant growth for sustainable agricultural practices under the influence of multiple abiotic stresses such as Cr(VI), high salinity, and artificial drought condition. The study investigated various factors influencing IAA synthesis by SSAU-2, such as pH (5 to 11), salinity (10 to 50 g/L), tryptophan concentration (0.5 to 1%), carbon (mannitol mand lactose), and nitrogen sources (peptone and tryptone). The highest IAA concentration was observed at pH 10 (1.695 mg/ml) and pH 11 (0.782 mg/ml). IAA synthesis was optimized at a salinity level of 30 g/l, with lower and higher salinity levels resulting in decreased IAA concentrations. Notably, the presence of mannitol and lactose significantly augmented IAA synthesis, while glucose and sucrose had inhibitory effects. Furthermore, peptone and tryptone played a pivotal role in enhancing IAA synthesis, while ammonium chloride exerted an inhibitory influence. SSAU-2 showed a diverse array of capabilities, including the synthesis of gibberellins, extracellular polymeric substances, siderophores, and hydrogen cyanide along with nitrogen fixation and ammonia production. The microbe could efficiently tolerate 45% PEG-6000 concentration and effectively produce IAA in 15% PEG concentration. It could also tolerate high concentration of Cr(VI) and synthesize IAA even in 50 ppm Cr(VI). The findings of this study provide valuable insights into harnessing the potential of indigenous microorganisms to promote plant growth, enhance soil fertility, and establish sustainable agricultural practices essential for restoring the health of ecosystems.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":"81-93"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140904164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trends in serotype distribution and antimicrobial susceptibility pattern of invasive Haemophilus influenzae isolates from Brazil, 2009-2021.","authors":"Rosemeire Cobo Zanella, Sérgio Bokermann, Marta Galhardo, Caroline Gava, Samanta Cristine Grassi Almeida, Gabriela Andrade Pereira, Ana Paula Silva de Lemos","doi":"10.1007/s10123-024-00535-5","DOIUrl":"10.1007/s10123-024-00535-5","url":null,"abstract":"<p><strong>Introduction: </strong>Invasive Haemophilus influenzae (Hi) disease poses a significant global health challenge. With the relaxation of COVID-19 pandemic measures and declining H. influenzae serotype b (Hib) vaccination coverage, there is concern about a potential increase in Hi cases worldwide.</p><p><strong>Methodology: </strong>This study analyzed 1437 invasive Hi isolates in Brazil over 13 years, determining capsular serotypes, antimicrobial susceptibility, and genetic relatedness through multilocus sequence typing.</p><p><strong>Results: </strong>The primary source of isolation for these invasive H. influenzae isolates was blood (54.4%), followed by cerebrospinal fluid (37.1%) and lung specimens (8.5%), respectively. Consequently, bacteremia (47%) was the most common clinical presentation, followed by meningitis (39.6%) and pneumonia (13.4%). Non-encapsulated Hi (NTHi) predominated among the isolates (51.4%), along with serotype a (22%) and serotype b (21.5%) among the encapsulated isolates. The majority of the encapsulated isolates were isolated from children under 14 years of age (76.7%), while NTHi isolates were identified in patients older than 15 years, particularly those ≥ 60 years old (40%). Ampicillin resistance was observed in 17.1% of cases, displaying β-lactamase production as the principal resistance mechanism. MLST revealed a diverse NTHi population, whereas the encapsulated isolates presented a clonal structure.</p><p><strong>Conclusion: </strong>This study describes the prevalence of NTHi isolates circulating in Brazil after two decades of the Hib vaccine immunization program. Continuous universal surveillance is crucial for implementing prompt public health measures to prevent and control invasive Hi disease and monitor changes in antibiotic resistance profiles.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":"157-163"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140921267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome analysis reveals key genes and pathways associated with heat stress in Pleurotus pulmonarius.","authors":"Wang Weike, Lu Na, Lin Jiayao, Lai Liqin, Chen Guanping","doi":"10.1007/s10123-024-00536-4","DOIUrl":"10.1007/s10123-024-00536-4","url":null,"abstract":"<p><p>Pleurotus pulmonarius is a medium temperature edible mushroom, and its yield and quality are severely affected by high temperature. However, the molecular mechanism of Pleurotus pulmonarius response to heat stress remains unknown. In this study, transcriptome sequencing and analysis of Pleurotus pulmonarius mycelia under heat stress were performed, related differentially expressed genes (DEGs) were verified by fluorescence quantitative PCR (qPCR) and the reduced glutathione content was detected. 5906 DEGs, including 1086 upregulated and 4820 downregulated, were identified by RNA-Seq. GO analysis revealed that DEGs were mainly enriched in the pathways of Aminoacyl-tRNA biosynthesis, pyrimidine metabolism, arginine and proline metabolism, fructose and mannose metabolism, and glutathione metabolism. qPCR analysis showed that the expression of ggt decreased after heat stress treatment, while gst2 and gst3 increased. The glutathione content in mycelia after heat stress was significantly higher than that in the control group. These results suggest that glutathione metabolism may play an important role in the response to heat stress. Our study will provide a molecular-level perspective on fungal response to heat stress and a basis for research on fungal environmental response and molecular breeding.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":"165-172"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140945083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}