International Microbiology最新文献

{"title":"Microbial consortium involving Pseudomonas and Bacillus: strain selection and the effect of co-cultivation on biocontrol activity against phytopathogens and the composition of metabolic extracts.","authors":"João Gabriel Dumont Negrelli, Maira Rafaela de Britto Rafael, Vitor Dib Gazola, Marcus Cesar Bochi Reis Dos Santos, Eduardo Jorge Pilau, Andressa Domingos Polli, Halison Correia Golias, Tiago Tognolli de Almeida, Julio Cesar Polonio","doi":"10.1007/s10123-025-00668-1","DOIUrl":"https://doi.org/10.1007/s10123-025-00668-1","url":null,"abstract":"<p><p>Agricultural sustainability is vital to meet the growing global demand for food; therefore, the search for more sustainable options to replace traditional chemical products has gained attention due to their benefits. The sector has applied innovative microbial consortium approaches as a niche for exploring new bioproducts and metabolic pathways through microbial interactions. Thus, this study sought to select two endophytic bacterial strains with biocontrol activity to study their metabolic interactions in culture. For this, co-cultivation and axenic culture assays were carried out to evaluate the inhibition of Corynespora cassiicola, Sclerotinia sclerotiorum, Moniliophthora perniciosa, and Colletotrichum truncatum. After the production of antiphytopathogenic compound tests, two strains were selected: P. putida MG36 and B. amyloliquefaciens SS14. These bacteria were cultivated under three distinct conditions: axenic cultivation of SS14, axenic cultivation of MG36, and co-cultivation. The metabolites were extracted and analyzed by liquid chromatography coupled with mass spectrometry (UHPLC-MS/MS). The results showed that both strains exhibited significant antifungal activity. B. amyloliquefaciens SS14 demonstrated 65% inhibition against C. truncatum, while P. putida MG36 showed 58% inhibition against S. sclerotiorum. Analysis of the chemical profiles revealed the presence of exclusive and shared metabolites, such as iturin A4 (antifungal lipopeptide) and macrolactin A (bioactive polyketide), under different culture conditions. In conclusion, P. putida MG36 and B. amyloliquefaciens SS14 show promise as biocontrol agents against phytopathogens, contributing to more sustainable agricultural practices.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144018426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic characterization of a novel Pseudomonas aeruginosa bacteriophage representing the newly proposed genus Angoravirus: in vitro antimicrobial and antibiofilm activity.","authors":"Sezin Unlu, Aylin Uskudar Guclu","doi":"10.1007/s10123-025-00669-0","DOIUrl":"https://doi.org/10.1007/s10123-025-00669-0","url":null,"abstract":"<p><p>Multi-drug-resistant (MDR) Pseudomonas aeruginosa is an important pathogen that poses a critical threat due to its metallo-beta-lactamase (MBL)-mediated carbapenem resistance and biofilm-forming ability, making bacterial treatment very complicated and requiring alternative strategies. Bacteriophages are promising alternatives; however, the discovery of novel phages targeting MDR strains remains urgent. In this study, Pseudomonas phage Baskent_P4_1, a novel virulent siphovirus that infects clinical MDR P. aeruginosa isolates, was isolated from wastewater and characterized comprehensively. Its efficacy was tested against biofilm-forming, MDR isolates with MBL activity by spot test and efficiency of plating (EOP). Biological characterization showed that phage Baskent_P4_1 is stable at pH 4-10 and temperatures up to 50 °C, while its stability decreases  >60 °C temperature. It has a short latent period of 10 min and a high burst size of 253 phages per cell. The phage lysed 40% of the MDR P. aeruginosa isolates tested, including strong biofilm producers. In vitro assays showed significant biofilm inhibition (48.8% reduction at 10⁹ PFU/mL) and degradation of pre-formed biofilms. Transmission electron microscopy (TEM) revealed an icosahedral head (70 nm) and a long non-contractile tail (150 nm). Whole genome sequencing by Illumina demonstrated a linear dsDNA genome of 41.947 bp (62.8% GC content) with 53 predicted coding sequences. No virulence factors, antibiotic resistance genes, or tRNAs were detected, thus ensuring therapeutic safety. Along with phylogenetic and vConTACT2 analysis, these results suggested that phage Baskent_P4_1 belongs to a new genus, which was proposed here as the genus Angoravirus with three other species. Genomic analysis identified hydrolases (ORF 13/14) and 7-deazaguanine modification enzymes (ORF 46/47) that may contribute to host lysis and evasion of bacterial defenses. These findings highlight Baskent_P4_1's potential as a therapeutic candidate against MDR P. aeruginosa infections. The study underscores the importance of expanding phage diversity libraries and provides a framework for characterizing novel phages to combat antimicrobial resistance.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144012022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbiome-mediated flavor development in Berbassa: a traditional fermented milk starter for Gergoush production.","authors":"Sara A Eltigani, Takeshi Taniguchi, Atsushi Ishihara, Jiro Arima, Elgasim A Elgasim, Mohamed M Eltayeb","doi":"10.1007/s10123-025-00665-4","DOIUrl":"https://doi.org/10.1007/s10123-025-00665-4","url":null,"abstract":"<p><p>Berbassa is a traditional fermented milk starter used to produce Gergoush, a dry snack that is popular in the dryland regions of Sudan. The unique flavor of Gergoush primarily depends on the spontaneous anaerobic fermentation of milk by natural microbiota following the addition of legumes. Therefore, this study aimed to investigate the microbial communities and volatile compounds in Berbassa prepared using chickpeas and cumin. The microbial community and volatile compounds in the non-fermented milk and Berbassa samples were analyzed using high-throughput sequencing and GC-MS, respectively. The bacterial community in Berbassa samples was dominated by Bacillus (31.52-53.37%) and Clostridium sensu stricto 1 (17.58-57.03%), followed by Paenibacillus, Brevibacillus, and Paraclostridium. In contrast, most fungal genera remained unidentified, followed by Trichosporon, Nothophoma, Alternaria, Aspergillus, and Mortierella as the most prominent identified genera. Fifty-seven compounds were detected by GC-MS, including esters, aldehydes, acids, ketones, alcohols, and phospholipids. Correlation analysis revealed that Bacillus, Clostridium sensu stricto 1, Paraclostridium, Phoma, and Piloderma were significantly correlated with several volatile compounds detected in Berbassa samples. Among these, several compounds are well-known for their strong flavors. The findings suggest a potential connection between the dominant genera Bacillus and Clostridium and the detected volatile compounds, which may contribute to the pungent flavor of Gergoush. This study provides valuable insights for future research on the contribution of individual strains to the unique flavor of Berbassa.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143995386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of cultivation conditions in hydrogel systems to enhance Chlorella vulgaris growth.","authors":"Guangtao Yang, Mingyang Li, Jinglin Zhang, Jindao Zhou, Wai Yan Cheah, Rosazlin Abdullah, Tau Chuan Ling","doi":"10.1007/s10123-025-00670-7","DOIUrl":"https://doi.org/10.1007/s10123-025-00670-7","url":null,"abstract":"<p><p>Microalgae have gained significant attention as sustainable alternatives to traditional agriculture and energy resources. The cultivation efficiency of microalgae within hydrogel systems presents a promising avenue for spatially efficient bio-production. However, the optimum cultivation conditions of hydrogel cultivation systems have not been elucidated. This study focused on evaluating the hydrogel-based cultivation of Chlorella vulgaris in symbiosis with Bacillus Strain Salmah Ismail (SI) 139SI. It investigated the impact of hydrogel concentration, pH, light exposure, and system thickness on the growth and chlorophyll production of the algae. Our findings highlighted that, in coculture, a 7% (w/v) hydrogel concentration, pH of 7.4, a 12-h light/dark cycle at a hydrogel concentration of 7% but continuous light exposure under 5% hydrogel concentration, and a system thickness of 10 mm have provided the most favorable environmental conditions for the proliferation and chlorophyll production of C. vulgaris. These conditions significantly enhanced the biomass yields, suggesting that tailored hydrogel environments can substantially improve microalgae productivity.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144002105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lactiplantibacillus plantarum as a sustainable solution for monocrotophos degradation and plant growth enhancement.","authors":"Archana Kumari, Chiranjit Ghosh, N Kannan, S Balaji","doi":"10.1007/s10123-025-00671-6","DOIUrl":"https://doi.org/10.1007/s10123-025-00671-6","url":null,"abstract":"<p><p>In the present study, characterization of bacterial isolate from fermented foods based on the morphology, biochemical, and molecular analysis identified Lactiplantibacillus plantarum. The bacterium was tested for its ability to degrade monocrotophos (MCP) at a concentration of 1000 ppm. The dephosphorylation potential of this bacterial enzyme against organophosphate substrates including MCP was analyzed. The Lactiplantibacillus plantarum phosphatase showed optimal activity at 40 °C and 6 pH. The Michaelis-Menten constant was found to be 238.14 µM while Vmax is 357.14 µmol/min.ml for MCP as a substrate. The enzyme activity was enhanced by metal ions such as Mn²⁺, Mg²⁺, and Ca²⁺ and inhibited by K⁺, Zn²⁺, and Fe²⁺ ions. Plant growth promotion assays of Lp. plantarum exhibited characteristic traits including phosphate solubilization, cellulase, catalase, and xylanase activities. The degradation of MCP was confirmed based on the presence of acetamide and trimethyl phosphate as intermediate metabolites, confirmed by FTIR and GC-MS analysis. Thus, L. plantarum is a suitable candidate for phosphate solubilization, plant growth promotion, and bio-fertilizer application.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143989728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Arsenic stress management through arsenite and arsenate-tolerant growth-promoting bacteria in rice.","authors":"Basudev Majhi, Pradeep Semwal, Shashank Kumar Mishra, Sankalp Misra, Puneet Singh Chauhan","doi":"10.1007/s10123-023-00447-w","DOIUrl":"10.1007/s10123-023-00447-w","url":null,"abstract":"<p><p>Arsenic (As) contamination is a major problem affecting soil and groundwater in India, harming agricultural crops and human health. Plant growth-promoting rhizobacteria (PGPR) have emerged as promising agents for reducing As toxicity in plants. This study aimed to isolate and characterize As-tolerant PGPR from rice field soils with varying As levels in five districts of West Bengal, India. A total of 663 bacterial isolates were obtained from the different soil samples, and 10 bacterial strains were selected based on their arsenite (As-III) and arsenate (As-V) tolerant ability and multiple PGP traits, including phosphate solubilization, production of siderophore, indole acetic acid, biofilm formation, alginate, and exopolysaccharide. These isolates were identified by 16S rRNA gene sequencing analysis as Staphylococcus sp. (4), Niallia sp. (2), Priestia sp. (1), Bacillus sp. (1), Pseudomonas sp. (1), and Citricoccus sp. (1). Among the selected bacterial strains, Priestia flexa NBRI4As1 and Pseudomonas chengduensis NBRI12As1 demonstrated significant improvement in rice growth by alleviating arsenic stress under greenhouse conditions. Both strains were also able to modulate photosynthetic pigments, soluble sugar content, proline concentration, and defense enzyme activity. Reduction in As-V accumulation inoculated with NBRI4As1 was recorded highest by 53.02% and 31.48%, while As-III by NBRI12As1 38.84% and 35.98% in the roots and shoots of rice plants, respectively. Overall, this study can lead to developing efficient As-tolerant bacterial strains-based bioinoculant application packages for arsenic stress management in rice.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":"11-25"},"PeriodicalIF":2.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136397393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Bacillus isolates as a biological control agents against soilborne phytopathogenic fungi.","authors":"Derya Maral-Gül, Rengin Eltem","doi":"10.1007/s10123-024-00490-1","DOIUrl":"10.1007/s10123-024-00490-1","url":null,"abstract":"<p><p>Pesticides, used in agriculture to control plant diseases, pose risks to the environment and human health. To address this, there's a growing focus on biocontrol, using microorganisms instead of chemicals. In this study, we aimed to identify Bacillus isolates as potential biological control agents. We tested 1574 Bacillus isolates for antifungal effects against pathogens like Botrytis cinerea, Fusarium solani, and Rhizoctonia solani. Out of these, 77 isolates formed inhibition zones against all three pathogens. We then investigated their lytic enzyme activities (protease, chitinase, and chitosanase) and the production of antifungal metabolites (siderophore and hydrogen cyanide). Coagulase activity was also examined to estimate potential pathogenicity in humans and animals. After evaluating all mechanisms, 19 non-pathogenic Bacillus isolates with significant antifungal effects were chosen. Molecular identification revealed they belonged to B. subtilis (n = 19) strains. The 19 native Bacillus strains, demonstrating strong antifungal effects in vitro, have the potential to form the basis for biocontrol product development. This could address challenges in agricultural production, marking a crucial stride toward sustainable agriculture.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":"75-89"},"PeriodicalIF":2.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139905634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The protective effect of L-theanine addition to a cryoprotectant on the storage viability of lactic acid bacteria.","authors":"Hyuk-Ju Kwon, Trung Hau Nguyen, Jin-Seong Kim, Chang-Ho Kang","doi":"10.1007/s10123-023-00444-z","DOIUrl":"10.1007/s10123-023-00444-z","url":null,"abstract":"<p><p>Lactic acid bacteria (LAB) that metabolize sugars to obtain energy and produce a large amount of lactate through the process are well known for their benefits. However, they can be used on a large scale only when good storage stability is guaranteed. The vitality and stability of several LAB strains were effectively protected in this investigation by L-theanine at 1% of the appropriate concentration (Lactiplantibacillus plantarum MG5023, Enterococcus faecium MG5232, Lactococcus lactis MG4668, Streptococcus thermophilus MG5140, and Bifidobacterium animalis subsp. lactis MG741). The inclusion of L-theanine as a protective agent significantly enhanced the viability of all strains throughout the freeze-drying process compared to that of the non-coated probiotics. The efficacy of L-theanine in improving bacterial stability and survivability was evaluated using accelerated stability tests, gastrointestinal (GI) tract survivability tests, and adhesion assays with intestinal epithelial cells. The cell surface was covered with substances including L-theanine, according to morphological findings, providing efficient defense against a variety of external stresses. Therefore, by exerting anti-freezing and anti-thawing properties, the adoption of L-theanine as a new and efficient protective agent may improve the stability and viability of a variety of probiotics.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":"45-52"},"PeriodicalIF":2.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138434011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the antifungal potential of genetically modified hybrid chitinase enzymes derived from Bacillus subtilis and Serratia marcescens.","authors":"Nahla Alsayd Bouqellah, Lina Jamil Mohamed Abdel-Hafez, Islam Yousif Mostafa, Ahmed Hassan Ibrahim Faraag","doi":"10.1007/s10123-024-00591-x","DOIUrl":"10.1007/s10123-024-00591-x","url":null,"abstract":"<p><p>Chitinases are glycosyl hydrolase enzymes that break down chitin, an integral component of fungal cell walls. Bacteria such as Bacillus subtilis and Serratia marcescens produce chitinases with antifungal properties. In this study, we aimed to generate hybrid chitinase enzymes with enhanced antifungal activity by combining functional domains from native chitinases produced by B. subtilis and S. marcescens. Chitinase genes were cloned from both bacteria and fused together using overlap extension PCR. The hybrid constructs were expressed in E. coli and the recombinant enzymes purified. Gel electrophoresis and computational analysis confirmed the molecular weights and isoelectric points of the hybrid chitinases were intermediate between the parental enzymes. Antifungal assays demonstrated that the hybrid chitinases inhibited growth of the fungus Fusarium oxysporum significantly more than the native enzymes and also showed fungicidal activity against Candida albicans, Alternaria solani, and Rhizoctonia solani. The results indicate that hybrid bacterial chitinases are a promising approach to engineer novel antifungal proteins. This study provides insight into structure-function relationships of chitinases and strategies for generating biotherapeutics with enhanced bioactive properties. These hybrid chitinases result in a more potent and versatile antifungal agent.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":"177-194"},"PeriodicalIF":2.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142361486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alleviation of heavy metals chromium, cadmium and lead and plant growth promotion in Vigna radiata L. plant using isolated Pseudomonas geniculata.","authors":"Barkha Madhogaria, Sangeeta Banerjee, Sohini Chakraborty, Prasanta Dhak, Atreyee Kundu","doi":"10.1007/s10123-024-00546-2","DOIUrl":"10.1007/s10123-024-00546-2","url":null,"abstract":"<p><p>Plants exposed to heavy metals (HMs) stress negatively affect their development and production capacity. Chromium (Cr), Cadmium (Cd), and Lead (Pb) are the most common hazardous trace metals in agriculture. The physiological, biochemical, and molecular characteristics of crops are being affected. Phytoremediation is a method to alleviate heavy metals from the contaminated soil. The study aims to evaluate the phytoremediation ability of Vigna radiata L. (mung bean) in the absence and the presence of multi-metal tolerant and plant growth promoting Pseudomonas geniculata strain TIU16A3 isolated from soil of tannery industrial estate, Kolkata, West Bengal, India. The strain was further assessed with increasing concentrations of Cr, Cd, and Pb (10, 20, 40, and 80 µg/mL) when the mung bean plant was a test crop. The strain significantly increased plant growth, chlorophyll content, increased level of antioxidant enzymes such as superoxide dismutase, peroxidase, and catalase, and decreased oxidative stress indicators like H₂O₂ and electrolyte leakage in the presence of Cr, Cd, and Pb as compared to plants grown in the absence of Pseudomonas geniculata strain. Shoot length responsive gene (Aux/IAA) in the presence of heavy metal alone and Pseudomonas geniculata treated Cd and Cr showed higher relative expression of (Aux/IAA) compared to Pb. Due to these intrinsic abilities, Pseudomonas geniculata strain TIU16A3 can be a plant growth promoter and thus can help in the remediation of heavy metal (Cr, Cd, and Pb) contaminated soil.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":"133-149"},"PeriodicalIF":2.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141446125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}