International Microbiology最新文献_第2页

Impacts of different salt concentrations on the morphology, physiology, and antioxidant properties of the rice-field cyanobacterium Nostochopsis lobatus HKAR-21.

IF 2.3 4区生物学

International Microbiology Pub Date : 2025-02-15 DOI: 10.1007/s10123-025-00638-7

Ashish P Singh, Amit Gupta, Jyoti Jaiswal, Prashant R Singh, Rajeshwar P Sinha

{"title":"Impacts of different salt concentrations on the morphology, physiology, and antioxidant properties of the rice-field cyanobacterium Nostochopsis lobatus HKAR-21.","authors":"Ashish P Singh, Amit Gupta, Jyoti Jaiswal, Prashant R Singh, Rajeshwar P Sinha","doi":"10.1007/s10123-025-00638-7","DOIUrl":"https://doi.org/10.1007/s10123-025-00638-7","url":null,"abstract":"The physiological and biochemical responses of organisms, including cyanobacteria, are greatly influenced by environmental change. Salinity significantly affects global rice output, and cyanobacteria are major contributors to rice productivity. Therefore, in our study, we explore the effects of varying concentrations of three (i.e., MgSO4, CaCl2, K2HPO4) agriculturally important salts on the biomass, growth, and biochemical status of the rice-field cyanobacterium Nostochopsis lobatus HKAR-21. A significant decrease in the photopigment activity, growth, and effective quantum yield of photosystem II (PSII) (Fv/Fm) was observed in the cells treated with 0 μM K2HPO4 and 0 μM MgSO4. There was minimum effect on the growth and photopigment in samples treated without CaCl2 (0 μM CaCl2). Phycobiliproteins (PBPs) and carbohydrate content were largely regulated by MgSO4. However, K2HPO4 had an optimum effect on all three macromolecular components. Brightfield imaging revealed that each salt had a characteristic role in the morphology of the cells. Additionally, in vitro lipid peroxidation with in vivo reactive oxygen species (ROS) and lipid peroxidation were examined with the help of DCFH-DA and Nile red fluorescence, respectively. The antioxidant potential of the carotenoid was checked by using DPPH and ABTS assays. Our study conclusively highlights that the increasing salt concentration will have a negative impact on the cyanobacterium growth which in turn will affect the productivity of rice paddy fields which is one of the most important crops on which more than a billion people are dependent.","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143425453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cloning, expression, purification, and characterization of glutamate decarboxylase (Rv3432c) from Mycobacterium tuberculosis.

IF 2.3 4区生物学

International Microbiology Pub Date : 2025-02-15 DOI: 10.1007/s10123-025-00637-8

Rupal Rai, Ruchi Paroha, Sandesh Rai, Anirudh K Singh, Rashmi Chaurasia, Nisheeth Agarwal, Megha Katare Pandey, Shivendra K Chaurasiya

{"title":"Cloning, expression, purification, and characterization of glutamate decarboxylase (Rv3432c) from Mycobacterium tuberculosis.","authors":"Rupal Rai, Ruchi Paroha, Sandesh Rai, Anirudh K Singh, Rashmi Chaurasia, Nisheeth Agarwal, Megha Katare Pandey, Shivendra K Chaurasiya","doi":"10.1007/s10123-025-00637-8","DOIUrl":"https://doi.org/10.1007/s10123-025-00637-8","url":null,"abstract":"Glutamate decarboxylase (Gad), a pyridoxal 5'-phosphate (PLP)-dependent enzyme, catalyzes the conversion of glutamate to γ-aminobutyric acid (GABA), consuming a proton in the process and thereby contributing to intracellular pH homeostasis in bacteria. However, the presence and function of the Gad-dependent mechanism in mycobacteria remain largely unexplored. This study aimed to characterize Gad activity in Mycobacterium tuberculosis (Mtb). We detected Gad activity in live cells of both Mtb and Mycobacterium smegmatis (MS). Gad activity and GABA was also detected in cell lysates of Mtb and MS. The gadB gene from Mtb was cloned, expressed, and GadB protein was purified under native conditions using MS as an expression host. Initial attempts to express GadB in Escherichia coli (E. coli) resulted in the overexpressed protein being present in the insoluble fraction and was enzymatically inactive when purified under denaturing conditions. Subsequently, an acetamide-inducible expression system was employed in MS for successful overexpression and purification of recombinant GadB. 6 × His-GadB was purified using immobilized metal affinity chromatography, and its molecular weight was determined to be ~ 51.2 kDa by SDS-PAGE. The purified 6 × His-GadB enzyme was active at both neutral and acidic pH. Its activity was found to be PLP-dependent, with optimal activity at pH 7.2 and 50°C. These findings suggest that Gad is expressed in Mtb both in normal and in acidic medium, supporting the possible existence of a Gad-dependent acid resistance mechanism in Mtb.","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143425448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Toxicity potential assessment of silicon dioxide (SiO₂) and zinc oxide (ZnO) on green microalgae Chlamydomonas sp. strain GO1.

IF 2.3 4区生物学

International Microbiology Pub Date : 2025-02-13 DOI: 10.1007/s10123-025-00635-w

Oumayma Ghariani, Jihen Elleuch, Bochra Gargouri, Faiza Fakhfakh, Chiara Bisio, Imen Fendri, Matteo Guidotti, Slim Abdelkafi

{"title":"Toxicity potential assessment of silicon dioxide (SiO2) and zinc oxide (ZnO) on green microalgae Chlamydomonas sp. strain GO1.","authors":"Oumayma Ghariani, Jihen Elleuch, Bochra Gargouri, Faiza Fakhfakh, Chiara Bisio, Imen Fendri, Matteo Guidotti, Slim Abdelkafi","doi":"10.1007/s10123-025-00635-w","DOIUrl":"https://doi.org/10.1007/s10123-025-00635-w","url":null,"abstract":"Due to the variety of applications for nanomaterials (NMs) and ultrafine solids, their amounts released into the environment is constantly increasing, and their impact on ecosystems and organisms has led to remarkable problems. However, extensive studies on the effects of dispersed ultrafine inorganic metal oxides on algal growth at cellular and genomic levels still need to be performed. We assessed the potential toxicity of two commercial ultrafine inorganic metal oxides, silicon dioxide (SiO2) and zinc oxide (ZnO), using the single-celled green microalgae Chlamydomonas sp. strain GO1 as a eukaryotic model. The cell response to commercial inorganic oxides was evaluated at physiological, biochemical, and molecular levels. An estimation of population growth inhibition levels was made. After 72 h of exposure, the IC50 of SiO2 and ZnO were 14.50 ± 2.98 mg/L and 56.80 ± 8.3 mg/L, respectively. Genotoxic effects of the studied materials were evaluated by acridine orange staining method and showed DNA fragmentation and morphological changes, including cell shrinkage and chromatin condensation on microalgae cells treated with both oxide materials. In addition, generated cytotoxic effects were evaluated. An inhibition of microalgae growth and a decrease in cell viability were observed. Antioxidant defense mechanisms, including enzymatic and non-enzymatic, were activated in response to materials exposure. We have also proven an overexpression of genes involved in carbohydrate biosynthesis and apoptosis. Infrared investigation suggested surface chemical interaction between algal cells and commercial ultrafine inorganic oxides.","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143407918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antagonistic activity of butanamine 2,2-dinitro-N-methyl- synthesized by endosymbiotic Bacillus amyloliquefaciens VITAPRJS1 acquired from horse milk.

IF 2.3 4区生物学

International Microbiology Pub Date : 2025-02-13 DOI: 10.1007/s10123-025-00640-z

Arnab Chakraborty, Prerona Dutta, Rajshree Amrit, Parry Dey, W Jabez Osborne

{"title":"Antagonistic activity of butanamine 2,2-dinitro-N-methyl- synthesized by endosymbiotic Bacillus amyloliquefaciens VITAPRJS1 acquired from horse milk.","authors":"Arnab Chakraborty, Prerona Dutta, Rajshree Amrit, Parry Dey, W Jabez Osborne","doi":"10.1007/s10123-025-00640-z","DOIUrl":"https://doi.org/10.1007/s10123-025-00640-z","url":null,"abstract":"Endosymbiotic bacteria are known to synthesize bioactive compounds which have biotechnological potentials that enhance immune responses by stimulating the production of immune cells. Horse milk is widely known to have nutraceutical and antimicrobial activities; however, there are no scientific reports on its inhibitory effects. VITAPRJS1, isolated from horse milk, showed non-hemolytic properties and was significantly tolerant to bile salt and NaCl. The isolate also exhibited potent antibacterial activity against pathogenic bacterial strains such as Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Bacillus cereus. The bioactive antibacterial compounds were extracted using dichloromethane and were subsequently purified and identified as butanamine, 2,2-dinitro-N-methyl- through UPLC, GC-MS, and LC-MS analyses. Fourier transform infrared spectroscopy (FTIR) confirmed the presence of functional groups such as alkane, amine, and monosubstituted 1,2-disubstituted. The screened bacterial isolate was identified as Bacillus amyloliquefaciens (OR501558) upon 16S rRNA gene sequencing. To our knowledge, this study represents the first-time report on the presence of Bacillus amyloliquefaciens in horse milk having potent antibacterial activity, highlighting its unexplored potential in biotechnological and pharmaceutical applications.","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143407915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Larvicidal and cytotoxic activities of exopolysaccharides produced by thermophilic bacteria.

IF 2.3 4区生物学

International Microbiology Pub Date : 2025-02-12 DOI: 10.1007/s10123-025-00644-9

Mehmet Aytar, Demet Yalçın Bingül, Mustapha Touray, Deniz Aktaş Uygun, Gamze Başbülbül

{"title":"Larvicidal and cytotoxic activities of exopolysaccharides produced by thermophilic bacteria.","authors":"Mehmet Aytar, Demet Yalçın Bingül, Mustapha Touray, Deniz Aktaş Uygun, Gamze Başbülbül","doi":"10.1007/s10123-025-00644-9","DOIUrl":"https://doi.org/10.1007/s10123-025-00644-9","url":null,"abstract":"Exopolysaccharides (EPSs) synthesized by thermophilic bacteria are natural biopolymers, that have recently garnered attention due to their potential applications in areas such as pharmaceuticals and biomedicine. In this study, EPSs produced by five distinct thermophilic bacterial isolates from hot springs in Turkey were purified using ion exchange and gel chromatography, and the larvicidal and cytotoxic effects of these EPSs were examined. While Geobacillus thermodenitrificans HBB111 produced the highest quantity (650.9 mg mL-1) of EPS, the protein content of crude EPS samples ranged from 0.3 to 1.5%. EPS111 and EPS261 showed the most effective larvicidal action, eliminating 72% and 62.7% of Ae. albopictus larvae after 48 h, respectively. Among the purified samples, EPS111 exhibited the most significant effect on the proliferation of PC3 cells, resulting in a 68% inhibition (IC50 of 0.23 mg mL-1) followed by EPS106 in 55% (IC50 of 0.45 mg mL-1). According to our study's results, thermophilic EPSs show promise because of their insecticidal and anticancer properties.","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143399101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biodegradation of tetracycline by Cladosporium colombiae T1: performance and degradative pathway.

IF 2.3 4区生物学

International Microbiology Pub Date : 2025-02-11 DOI: 10.1007/s10123-025-00643-w

Yujun Ma, Jihong Wang, Su Leng

{"title":"Biodegradation of tetracycline by Cladosporium colombiae T1: performance and degradative pathway.","authors":"Yujun Ma, Jihong Wang, Su Leng","doi":"10.1007/s10123-025-00643-w","DOIUrl":"https://doi.org/10.1007/s10123-025-00643-w","url":null,"abstract":"The microbial degradation of tetracycline (TC) represents an effective bioremediation method. An effective TC-degrading strain of Cladosporium colombiae T1 was isolated from chicken manure using enrichment techniques. Response surface methodology was employed to ascertain the optimal conditions for removing TC by strain T1, identified as a temperature of 40.00 °C, solution pH of 6.92, TC concentration of 42.99 mg/L, and inoculum dose of 1.98%. The inhibitory effect of TC degradation products on Escherichia coli was investigated using the paper diffusion method. The results demonstrated that the toxicity of TC degradation products by T1 was lower than that of the parent compound. The shake-flask batch experiments showed that the biodegradation of TC was a synergistic effect of intra- and extracellular enzymes, with intracellular enzymes exhibiting greater efficacy in TC degradation (48.56%). LC-MS analysis identified ten potential biodegradation products, and biodegradation pathways were proposed. This study offers a theoretical foundation for the characterization and mechanistic investigation of TC degradation in the environment by Cladosporium colombiae T1.","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143399098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Construction and application of soil-amending microbial inoculant for straw decomposition.

IF 2.3 4区生物学

International Microbiology Pub Date : 2025-02-07 DOI: 10.1007/s10123-025-00639-6

Hao Huang, Jihong Wang, Su Leng

{"title":"Construction and application of soil-amending microbial inoculant for straw decomposition.","authors":"Hao Huang, Jihong Wang, Su Leng","doi":"10.1007/s10123-025-00639-6","DOIUrl":"https://doi.org/10.1007/s10123-025-00639-6","url":null,"abstract":"The current body of research on using composite microbial inoculants in soil to promote straw decomposition, potassium solubility, phosphorus solubility, and crop growth is relatively limited. In this study, we developed a composite microbial inoculant, designated HC-2, through the enrichment of the field with two strains of Bacillus. The agent displays multifunctionality and promotes rot prevention, solubilization of potassium and phosphorus, growth stimulation, and disease suppression. To further investigate its effects on soil fertility and microbial communities, field experiments were conducted. The results led to the identification of the optimal preparation conditions for the HC-2 compound. In accordance with the aforementioned conditions, the agent demonstrated considerable cellulose-degrading enzyme activity, resulting in a 31.5% increase in the rate of straw degradation within 90 days. Furthermore, it exhibited pronounced effects on maize seedling growth and demonstrated exceptional potassium and phosphorus-solubilizing capabilities, and strong disease suppression ability. In conditions of straw return, HC-2 was observed to effectively improve soil fertility and optimize the soil micro-ecological environment, with the number of operational taxonomic units (OTUs) reaching 536. The abundance of Proteobacteria increased by 10.8%, while that of Acidobacteriota decreased by 10.93% and that of Bacillus increased by 1.05%. Additionally, soil transcriptional functions, signaling mechanisms, and inorganic ion transport metabolic functions were enhanced. This study demonstrates that the HC-2 microbial inoculant overcomes the limitations of single-strain agents and has significant application value in improving farmland soil quality.","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143364770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Brucella pituitosa strain BU72, a new hydrocarbonoclastic bacterium through exopolysaccharide-based surfactant production. 坑氏布鲁氏菌菌株 BU72，一种通过外多糖生产表面活性剂的新型碳氢化合细菌。

IF 2.3 4区生物学

International Microbiology Pub Date : 2025-02-01 Epub Date: 2024-06-12 DOI: 10.1007/s10123-024-00540-8

Mouna Mahjoubi, Hanene Cherif, Habibu Aliyu, Habib Chouchane, Simone Cappello, Mohamed Neifar, Francesca Mapelli, Yasmine Souissi, Sara Borin, Don A Cowan, Ameur Cherif

{"title":"Brucella pituitosa strain BU72, a new hydrocarbonoclastic bacterium through exopolysaccharide-based surfactant production.","authors":"Mouna Mahjoubi, Hanene Cherif, Habibu Aliyu, Habib Chouchane, Simone Cappello, Mohamed Neifar, Francesca Mapelli, Yasmine Souissi, Sara Borin, Don A Cowan, Ameur Cherif","doi":"10.1007/s10123-024-00540-8","DOIUrl":"10.1007/s10123-024-00540-8","url":null,"abstract":"Hydrocarbon and heavy metal pollution are amongst the most severe and prevalent environmental problems due to their toxicity and persistence. Bioremediation using microorganisms is considered one of the most effective ways to treat polluted sites. In the present study, we unveil the bioremediation potential of Brucella pituitosa strain BU72. Besides its ability to grow on multiple hydrocarbons as the sole carbon source and highly tolerant to several heavy metals, BU72 produces different exopolysaccharide-based surfactants (EBS) when grown with glucose or with crude oil as sole carbon source. These EBS demonstrated particular and specific functional groups as determined by Fourier transform infrared (FTIR) spectral analysis that showed a strong absorption peak at 3250 cm-1 generated by the -OH group for both EBS. The FTIR spectra of the produced EBS revealed major differences in functional groups and protein content. To better understand the EBS production coupled with the degradation of hydrocarbons and heavy metal resistance, the genome of strain BU72 was sequenced. Annotation of the genome revealed multiple genes putatively involved in EBS production pathways coupled with resistance to heavy metals genes such as arsenic tolerance and cobalt-zinc-cadmium resistance. The genome sequence analysis showed the potential of BU72 to synthesise secondary metabolites and the presence of genes involved in plant growth promotion. Here, we describe the physiological, metabolic, and genomic characteristics of Brucella pituitosa strain BU72, indicating its potential as a bioremediation agent.","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":"299-313"},"PeriodicalIF":2.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141310720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Piceatannol: a renaissance in antibacterial innovation unveiling synergistic potency and virulence disruption against serious pathogens. 皮夏单宁醇：抗菌创新的复兴，揭示了对严重病原体的协同效力和毒力破坏。

IF 2.3 4区生物学

International Microbiology Pub Date : 2025-02-01 Epub Date: 2024-05-20 DOI: 10.1007/s10123-024-00532-8

Abdulrahman E Koshak, Mahmoud A Elfaky, Dina A I Albadawi, Hossam M Abdallah, Gamal A Mohamed, Sabrin R M Ibrahim, Abdulrahim A Alzain, El-Sayed Khafagy, Eslam M Elsayed, Wael A H Hegazy

{"title":"Piceatannol: a renaissance in antibacterial innovation unveiling synergistic potency and virulence disruption against serious pathogens.","authors":"Abdulrahman E Koshak, Mahmoud A Elfaky, Dina A I Albadawi, Hossam M Abdallah, Gamal A Mohamed, Sabrin R M Ibrahim, Abdulrahim A Alzain, El-Sayed Khafagy, Eslam M Elsayed, Wael A H Hegazy","doi":"10.1007/s10123-024-00532-8","DOIUrl":"10.1007/s10123-024-00532-8","url":null,"abstract":"In the relentless battle against multi-drug resistant Gram-negative bacteria, piceatannol emerges as a beacon of hope, showcasing unparalleled antibacterial efficacy and a unique ability to disrupt virulence factors. Our study illuminates the multifaceted prowess of piceatannol against prominent pathogens-Proteus mirabilis, Pseudomonas aeruginosa, Acinetobacter baumannii, and Klebsiella pneumoniae. Notably, piceatannol demonstrated a remarkable ability to inhibit biofilm formation, reduce bacterial mobility, and diminish extracellular enzyme synthesis.Mechanistic insights into piceatannol's activity unraveled its impact on membrane potential, proton motive force, and ATP production. Furthermore, our study delved into piceatannol's anti-quorum sensing (QS) activity, showcasing its potential to downregulate QS-encoding genes and affirming its affinity to critical QS receptors through molecular docking. Crucially, piceatannol exhibited a low propensity for resistance development, positioning it as a promising candidate for combating antibiotic-resistant strains. Its mild effect on red blood cells (RBCs) suggests safety even at higher concentrations, reinforcing its potential translational value. In an in vivo setting, piceatannol demonstrated protective capabilities, significantly reducing pathogenesis in mice infected with P. aeruginosa and P. mirabilis. This comprehensive analysis positions piceatannol as a renaissance in antibacterial innovation, offering a versatile and effective strategy to confront the evolving challenges posed by resilient Gram-negative pathogens.","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":"213-225"},"PeriodicalIF":2.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141065089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic activity between conventional antifungals and chalcone-derived compound against dermatophyte fungi and Candida spp. 传统抗真菌药与查耳酮衍生化合物对皮真菌和念珠菌属的协同活性

IF 2.3 4区生物学

International Microbiology Pub Date : 2025-02-01 Epub Date: 2024-05-31 DOI: 10.1007/s10123-024-00541-7

Taiza Maschio-Lima, Thiago Henrique Lemes, Mariela Domiciano Ribeiro Marques, João Paulo Zen Siqueira, Bianca Gottardo de Almeida, Glaucia Rigotto Caruso, Marcia Regina Von Zeska Kress, Paulo de Tarso da Costa, Luis Octávio Regasini, Margarete Teresa Gottardo de Almeida

{"title":"Synergistic activity between conventional antifungals and chalcone-derived compound against dermatophyte fungi and Candida spp.","authors":"Taiza Maschio-Lima, Thiago Henrique Lemes, Mariela Domiciano Ribeiro Marques, João Paulo Zen Siqueira, Bianca Gottardo de Almeida, Glaucia Rigotto Caruso, Marcia Regina Von Zeska Kress, Paulo de Tarso da Costa, Luis Octávio Regasini, Margarete Teresa Gottardo de Almeida","doi":"10.1007/s10123-024-00541-7","DOIUrl":"10.1007/s10123-024-00541-7","url":null,"abstract":"The scarce antifungal arsenal, changes in the susceptibility profile of fungal agents, and lack of adherence to treatment have contributed to the increase of cases of dermatomycoses. In this context, new antimicrobial substances have gained importance. Chalcones are precursors of the flavonoid family that have multiple biological activities, have high tolerability by humans, and easy synthesis. In this study, we evaluated the in vitro antifungal activity, alone and in combination with conventional antifungal drugs, of the VS02-4'ethyl chalcone-derived compound against dermatophytes and Candida spp. Susceptibility testing was carried out by broth microdilution. Experiments for determination of the target of the compound on the fungal cell, time-kill kinetics, and toxicity tests in Galleria mellonella model were also performed. Combinatory effects were evaluated by the checkerboard method. Results showed high activity of the compound VS02-4'ethyl against dermatophytes (MIC of 7.81-31.25 μg/ml). The compound targeted the cell membrane, and the time-kill test showed the compound continues to exert gradual activity after 5 days on dermatophytes, but no significant activity on Candida. Low toxicity was observed at 250 mg/kg. Excellent results were observed in the combinatory test, where VS02-4'ethyl showed synergistic interactions with itraconazole, fluconazole, terbinafine, and griseofulvin, against all isolates tested. Although further investigation is needed, these results revealed the great potential of chalcone-derived compounds against fungal infections for which treatments are long and laborious.","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":"265-275"},"PeriodicalIF":2.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141179562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0