Borrelidin M: a new borrelidin derivative obtained from Streptomyces rochei VL-16 exhibited potent antibacterial and antiproliferative properties.

Abstract

The lookout for novel metabolites to overcome the threat imposed by antibiotic resistant pathogens is on the rise globally. The present study reports on the identification and characterisation of a new borrelidin derivative from Streptomyces rochei VL-16 strain that was isolated from the soil samples of Osudu Lake in Puducherry. The active compound produced by Streptomyces rochei VL-16 strain was purified sequentially by thin layer, column and high performance liquid chromatography techniques. The purified compound was subsequently identified as borrelidin through Q-TOF-HR-LCMS, FTIR and UV-Vis spectroscopy techniques. Based on integration proton NMR signals and HR-ESI-MS peak pattern, the compound was further identified as a new derivative of borrelidin. Its 3D structure was optimised using density functional theory. The new derivative obtained in this study was designated as borrelidin M (C₂₈H₄₁NO_5, Mol. wt.471.3 g/mole). This derivative was found to exhibit potent inhibitory effects against the foodborne pathogens including Clostridium perfringens, Aeromonas hydrophila, Staphylococcus aureus, Listeria monocytogenes and Yersinia enterocolitica. Also, borrelidin M exhibited anticancer and anti-metastatic effects against A549 cell line with an IC₅₀ value of 17.5 μM. Collectively, these findings indicated promising biomedical applications of this new derivative of borrelidin.