International Journal of Molecular and Cellular Medicine最新文献

{"title":"Potential Protective Effects of Cerium Oxide Nanoparticles on Ultraviolet-B Irradiated Fibroblast Cells.","authors":"Reza Fardid, Mansooreh Mostafavi Mendi, Gholamhassan Haddadi, Mohammad Ali Takhshid","doi":"10.22088/IJMCM.BUMS.14.3.828","DOIUrl":"10.22088/IJMCM.BUMS.14.3.828","url":null,"abstract":"<p><p>Increased environmental ultraviolet-B (UVB) exposure stimulates reactive oxygen species (ROS) overproduction, disrupts cellular redox balance, and contributes to skin disorders. Antioxidants inhibit autoxidation by neutralizing or suppressing free radicals. Certain nanomaterials, like cerium oxide nanoparticles (CNPs), function as antioxidants under specific conditions. Through their redox and catalytic properties, CNPs scavenge ROS, mitigate oxidative damage, and may help prevent skin injuries. While most research targets ionizing radiation, studies exploring CNPs under non-ionizing UVB remain limited. To address this, the study evaluates their photochemoprotective effects in UVB-exposed L929 fibroblasts. The 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT), cupric ion reducing antioxidant capacity (CUPRAC), dichloro-dihydro-fluorescein diacetate (DCFH-DA), and annexin V/propidium iodide (annexin V/PI) assays were used to evaluate cell viability, oxidative stress, and apoptosis. Cell viability was assessed using the MTT assay at CNP concentrations of 10, 50, and 100 μM and UVB intensities of 150-900 mJ/cm², selected based on prior physiologically relevant in vitro studies. At 600 mJ/cm² UVB, cell viability decreased by 45% (MTT assay). Treatment with 50 μM CNP significantly increased total antioxidant capacity relative to untreated controls (CUPRAC assay, p = 0.0018). CNP and UVB effects on ROS production and apoptosis were evaluated separately and in combination using DCFH-DA and annexin V/PI assays. Results show that pretreatment with CNPs before UVB radiation impedes cell apoptosis and reduces ROS, suggesting that CNPs mitigate UVB-induced oxidative damage in L929 cells by restoring oxidative balance through their redox activity and ROS-scavenging properties. These findings indicate CNP-based interventions may offer therapeutic strategies against UVB-induced skin and related disorders.</p>","PeriodicalId":14152,"journal":{"name":"International Journal of Molecular and Cellular Medicine","volume":"14 3","pages":"828-842"},"PeriodicalIF":0.8,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12664684/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145648595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing Osteogenesis in Dental Implants: ZNF710-AS1 and miR-146a-5p in Smad Signaling Pathway Activation.","authors":"Reza Mahmoudi Anzabi, Naghmeh Shenasa, Ali Jafarian, Amir Hossein Davodpour, Mehran Zareanshahraki, Mohammad Ghasemirad, Sajjad Rostamzadeh","doi":"10.22088/IJMCM.BUMS.14.3.964","DOIUrl":"10.22088/IJMCM.BUMS.14.3.964","url":null,"abstract":"<p><p>Investigations have shown that understanding the regulatory mechanisms of the osteogenic differentiation of periodontal ligament stem cells (PDLSCs) can significantly enhance the regenerative capacity of periodontal tissues and mitigate periodontal disorders. Improving osteogenesis in dental implants is complex, requiring better bone regeneration and integration with the implant surface. ZNF710-AS1 is identified as a key regulator of osteogenic differentiation via the BMP6/Smad pathway, with the overexpression of miR-146a-5p and miR-146b-5p inhibiting ZNF710-AS1's osteogenic effects. This study explores the role of the long noncoding RNA ZNF710-AS1 in PDLSC osteogenic differentiation and clarifies its molecular mechanisms. Results indicate that ZNF710-AS1 expression positively influences osteogenic differentiation, while miR-146a-5p has a suppressive effect. ZNF710-AS1 serves as a competing endogenous RNA (ceRNA) for miR-146a-5p, increasing BMP6 levels and activating the Smad signaling pathway. The study also highlights the role of miR-146a-5p in osteogenesis and inflammation in peri-implant tissues. Findings suggest miR-146a-5p promotes osteogenic differentiation and could be a potential biomarker for periodontal conditions. In summary, this research provided key insights into the molecular mechanisms of osteogenesis and advocates for targeting ZNF710-AS1 and miR-146a-5p to improve dental implant integration and regenerative strategies for periodontal tissues.</p>","PeriodicalId":14152,"journal":{"name":"International Journal of Molecular and Cellular Medicine","volume":"14 3","pages":"964-975"},"PeriodicalIF":0.8,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12664691/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145648613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vitamin E incorporated in Carboxymethyl Cellulose-Gelatin Hydrogel Increases Wound Healing.","authors":"Seyed Reza Mousavi, Mojtaba Rashidi, Azam Khedri, Maryam Kouchak, Majid Salehi, Ghorban Mohammadzadeh","doi":"10.22088/IJMCM.BUMS.14.3.808","DOIUrl":"10.22088/IJMCM.BUMS.14.3.808","url":null,"abstract":"<p><p>Improving wound care products to facilitate effective skin repair is very important. Hydrogels are promising polymer-based dressings that enhance wound healing. Vitamin E can improve skin injuries by increasing antioxidant capacity. This study aimed to construct an innovative hydrogel from carboxymethyl cellulose (CMC) and gelatin (Gel) containing vitamin E to enhance wound healing. Five unique hydrogel formulations were constructed by combination of CMC-Gel and 25, 50, 100, and 200 µL/mL of Vitamin E. Structural characteristics of hydrogels were assessed using scanning electron microscopy (SEM) and Fourier-transform infrared spectroscopy (FTIR). Biochemical properties of hydrogels, including swelling, weight loss, pH values, and blood compatibility, were evaluated by specific methods. The cytotoxicity effects of hydrogels on the NIH-3T3 fibroblasts were determined by MTT assay. The therapeutic potential of the hydrogels was investigated using a full-thickness wound model in Wistar rats. The constructed CMC-Gel-Vit E hydrogel had a porous structure characterized by interconnected voids measuring 73.15±9.61 µm, which is favorable for promoting cell migration. The cytotoxicity results showed no toxicity effects of hydrogels with and without VitE 100 µL/mL, also a survival rate of over 120% in cells after 72 h. In vivo data showed the CMC-Gel-Vit E hydrogel (91.36±8.23%) significantly increased the percentage of wound closure and re-epithelialization compared to the control group (68.31±13.59%). The results highlight the considerable potential of the CMC-Gel-VitE hydrogel as a viable option for skin regeneration and wound healing. This hydrogel exhibits substantial promise for use in clinical and therapeutic interventions.</p>","PeriodicalId":14152,"journal":{"name":"International Journal of Molecular and Cellular Medicine","volume":"14 3","pages":"808-827"},"PeriodicalIF":1.8,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12664696/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145648466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of RAGE gene polymorphisms (rs2070600 and rs2071288) with bone metabolism disturbances in patients with chronic kidney disease.","authors":"Asma Ali Awad, Gholamreza Dehghan, Hamid Tayebi Khosroshahi, Mahdi Haghi","doi":"10.22088/IJMCM.BUMS.14.4.1045","DOIUrl":"10.22088/IJMCM.BUMS.14.4.1045","url":null,"abstract":"<p><p>Chronic kidney disease (CKD) is frequently accompanied by disturbances in bone metabolism, leading to a heightened risk of fractures. The receptor for advanced glycation end-products (RAGE) plays a pivotal role in the pathogenesis of CKD. This case-control study included 50 CKD patients and 50 healthy controls to examine the association between RAGE gene polymorphisms (rs2070600 and rs2071288) and bone metabolism markers. Serum parathyroid hormone (PTH), alkaline phosphatase (ALP), and vitamin D levels were measured as indicators of bone metabolism. CKD patients exhibited significantly higher ALP (317.54 ± 21.95 U/L) and PTH (331.33 ± 17.11 pg/mL) levels, along with lower vitamin D (26.92 ± 6.64 ng/mL) compared to controls (all P < 0.05). The CC genotype of rs2070600 was more prevalent among patients (52%) and was associated with an increased risk of CKD (OR = 2.79, 95% CI: 1.21-6.39, P = 0.014). In contrast, no significant association was found between rs2071288 and CKD risk. Notably, individuals carrying CC or CT genotypes for rs2070600 and rs2071288 had higher levels of PTH and ALP, alongside significantly lower levels of vitamin D. The RAGE gene's rs2070600 polymorphism may be connected to abnormalities in bone metabolism and the advancement of CKD. These results indicate that rs2070600 genotyping may be useful in identifying high-risk patients; however, additional research is required to validate its clinical significance.</p>","PeriodicalId":14152,"journal":{"name":"International Journal of Molecular and Cellular Medicine","volume":"14 4","pages":"1045-1058"},"PeriodicalIF":1.8,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13092838/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147770911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pulmonary toxoplasmosis in a multiple myeloma patient: A Case report and literature review.","authors":"Asgar Baghernejad Nesheli, Somayeh Sadeghi, Mahdi Fakhar, Sara Ghandi, Sadegh Fattahi, Eissa Soleymani, Abbasali Eskandarian","doi":"10.22088/IJMCM.BUMS.14.4.1129","DOIUrl":"10.22088/IJMCM.BUMS.14.4.1129","url":null,"abstract":"<p><p>Pulmonary toxoplasmosis is a rare but potentially fatal opportunistic infection, most often reported in HIV-infected individuals. Its occurrence in non-HIV immunocompromised patients-particularly those with hematologic malignancies like multiple myeloma (MM)-is exceedingly rare and diagnostically challenging due to nonspecific clinical and radiologic features. While <i>Toxoplasma gondii</i> infection is typically mild in immunocompetent hosts, it can cause severe disease in immunocompromised populations. A literature review was conducted using PubMed, Scopus, and Google Scholar (January 2000-May 2025) with keywords: 'pulmonary toxoplasmosis,' 'multiple myeloma,' 'non-HIV,' 'immunocompromised,' and 'BAL PCR.' Only English-language case reports and reviews were included, with emphasis on studies from endemic regions and the past five years. We report a 49-year-old Iranian man with newly diagnosed IgG kappa MM who presented with fever, chills, vomiting, and dyspnea. Chest CT revealed a 4.2 × 3.8 cm consolidation in the posterior segment of the right lower lobe. T. gondii infection was confirmed via bronchoalveolar lavage (BAL) through tachyzoite detection on Giemsa stain and molecular identification using qPCR and nested PCR targeting the Gra6 gene. He showed transient clinical improvement and was discharged on hospital day 7. Unfortunately, he died at home within 48 hours due to MM progression, before anti-toxoplasmosis therapy was initiated. This case underscores the diagnostic difficulty of pulmonary toxoplasmosis in non-HIV immunocompromised patients and the importance of molecular BAL testing. Prophylactic measures such as co-trimoxazole should be considered in high-risk MM patients. Guidelines must address screening and management in this vulnerable population.</p>","PeriodicalId":14152,"journal":{"name":"International Journal of Molecular and Cellular Medicine","volume":"14 4","pages":"1129-1137"},"PeriodicalIF":1.8,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13092829/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147771002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression Analysis of <i>miR-20a-5p</i>, <i>miR-124-3P, miR-125b-5p, Resistin</i>, <i>TLR4</i>, <i>CD36</i> and <i>TNF-α</i> relationship in blood mononuclear cells from patients with atherosclerosis.","authors":"Maysam Mard Soltani, Ghazaleh Bagheri, Golnar Bakhtyar, Fatemeh Ahmadpour, Arash Amin, Gholamreza Shahsavari","doi":"10.22088/IJMCM.BUMS.14.4.1014","DOIUrl":"10.22088/IJMCM.BUMS.14.4.1014","url":null,"abstract":"<p><p>Atherosclerosis is the primary cause of death in developed nations. The key risk factors for atherosclerosis are inflammation and lipid disorders which may all be influenced by microRNAs (miRs). This study evaluated the correlation between <i>miR-20a-5p, miR-124-3p, miR-125b-5p</i>, and key atherogenic and inflammatory genes (<i>TLR4, Resistin, CD36, TNF-</i>α) in PBMCs from patients with angiography-proven atherosclerosis. 45 healthy individuals and 45 atherosclerosis patients were selected. After sampling patients and isolating in peripheral blood mononuclear cell (PBMC) cells, gene levels were measured using real-time polymerase chain reaction. In the atherosclerosis patient group, Toll-like receptor 4 (<i>TLR4</i>) and <i>Resistin</i> were upregulated compared to the control group (<i>p</i> <0.05). Conversely, <i>miR-20a</i> was downregulated in patients and inversely correlated with fasting blood glucose (<i>p</i> <0.05). Additionally, <i>miR</i>-<i>124</i> expression with Resistin had a significant negative correlation (<i>p</i> <0.05). The study confirmed that the expression level of <i>miR-125b</i> levels may serve as a potential biomarker for atherosclerosis (<i>p</i> <0.01). The reduction of <i>miR-20a</i> was related to the risk of developing atherosclerosis (<i>p</i> <0.05). <i>miR-20a</i>, <i>miR-124</i>, and <i>miR-125b</i> present promising therapeutic targets for atherosclerosis. Since atherosclerosis has no specific clinical symptoms and early diagnosis is very important, the diagnostic biomarker <i>miR-125b</i> has the potential to significantly aid in the early detection of various diseases with an area under the curve (AUC) of 0.74, 52% sensitivity, and 74% specificity. In addition, the measurement of <i>miR-20a</i> helps determine the progression of atherosclerosis risk.</p>","PeriodicalId":14152,"journal":{"name":"International Journal of Molecular and Cellular Medicine","volume":"14 4","pages":"1014-1030"},"PeriodicalIF":1.8,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13092834/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147771015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trace Amine-associated Receptor 1: Role in the Implementation of Neuroprotective Signaling Pathways.","authors":"Mikhail Voevoda, Dmitry Filimonov, Roman Knyazev, Roman Ishchenko, Alexander Eresko, Nadezhda Trubnikova, Margarita Belotserkovskaya, Irina Kisilenko, Inna Nosova, Maksim Solopov","doi":"10.22088/IJMCM.BUMS.14.4.980","DOIUrl":"10.22088/IJMCM.BUMS.14.4.980","url":null,"abstract":"<p><p>Trace amine-associated receptor 1 (TAAR1) is a classical representative of G-protein coupled receptors (GPCRs). It is widely distributed in the mammalian brain, potentially plays an important role in modulating neurotransmitter functions and may regulate synaptic transmission and neuronal activity. Studies on TAAR1 signaling pathways were reviewed to identify the potential of TAAR1 as a novel target for neuroprotection and neurorepair. TAAR1 realizes effects through binding to the G-protein subunits Gas or Ga13. The target of Gas is PKA and the target of Ga13 is RhoA. Among the RhoA-mediated effects of TAAR1, effects on MAPK/ERK pathway kinases, AMPA and NMDA glutamate receptors and CREB factor have been investigated. RhoA-mediated effects include effects on the internalization of DAT and EAAT3, neuronal transporters of dopamine and glutamate. A G-protein independent pathway is mediated by β-arrestin and is probably related to the formation of the TAAR1-D2R heterodimer. The modulatory effect of TAAR1 on neurotransmitter systems allows us to consider TAAR1 agonists as potential therapeutic agents for the treatment of neurodegenerative diseases and psychiatric disorders, as well as neuroprotectors in ischemic brain damage.</p>","PeriodicalId":14152,"journal":{"name":"International Journal of Molecular and Cellular Medicine","volume":"14 4","pages":"980-993"},"PeriodicalIF":1.8,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13092835/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147771036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic Efficacy of Dual-Target HSA-Based Delivery for HER and VEGFR Co-Blockade in Triple-Negative Breast Cancer.","authors":"Sadegh Rostaminasab, Sahar Memarkashani, Tahereh Rahdari, S Mohsen Asghari","doi":"10.22088/IJMCM.BUMS.14.4.1031","DOIUrl":"10.22088/IJMCM.BUMS.14.4.1031","url":null,"abstract":"<p><p>Resistance to monotherapies targeting receptor tyrosine kinases remains a major challenge in triplenegative breast cancer (TNBC). Here, we developed a dualtarget HSAbased nanobioconjugate combining human serum albumin (HSA)-encapsulated lapatinib, an EGFR1/2 inhibitor<b>,</b> with the VEGFR1/2blocking peptide VGB3 to achieve coordinated HER and VEGFR inhibition. Molecular docking predicted stable hydrogenbonding and hydrophobic interactions between Lapatinib (free and HSAbound) and EGFR1/2, and between VGB3 and VEGFR1/2. HSA encapsulation enabled pHresponsive, sustained drug release with faster kinetics under mildly acidic tumor conditions. Thermal denaturation assays confirmed an increase in the conformational stability of HSA after binding, indicating structural integrity and biocompatibility. In vitro studies revealed that HSALapatinib exhibited a ~55 % reduction in IC₅₀ (from ≈ 71 µM to ≈ 32 µM) compared with free Lapatinib. The combination of 17 µM HSALapatinib and 0.18 µM VGB3 reduced cell viability to 25 % (CI = 0.29), confirming a strong synergistic effect. Flow cytometric analysis demonstrated a significant increase in apoptosis-from 40.8 % (HSA-Lapatinib) to 46.8 % (combination) (<i>P < 0.05</i>). RTPCR further showed a fourfold upregulation of <i>VEGFR2</i> (<i>P < 0.0001</i>), supporting that HSALapatinib triggers compensatory <i>VEGFR2</i> activation which is neutralized by VGB3. Collectively, these findings substantiate a mechanistic and quantitative synergy between HSAmediated HER inhibition and VGB3based VEGFR blockade. This dualtarget HSALapatinib/VGB3 system offers enhanced potency, reduced resistance, and a promising platform for precisionguided TNBC therapy.</p>","PeriodicalId":14152,"journal":{"name":"International Journal of Molecular and Cellular Medicine","volume":"14 4","pages":"1031-1044"},"PeriodicalIF":1.8,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13092830/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147771042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association Between miR-146a rs2910164 Polymorphism and Tuberculosis Susceptibility: A Comprehensive Meta-Analysis.","authors":"Mohammad Yousef Alikhani, Fatemeh Khoobbakht, Salman Khazaei, Tahere Etesamifard, Sima Kazemi","doi":"10.22088/IJMCM.BUMS.14.4.1084","DOIUrl":"10.22088/IJMCM.BUMS.14.4.1084","url":null,"abstract":"<p><p>Prior research has indicated a potential link between the <i>miR-146a</i> rs2910164 genetic variant and an individual's susceptibility to pulmonary tuberculosis (TB). Nevertheless, the evidence from various studies is contradictory and has not yielded a consensus. This meta-analysis was therefore conducted to systematically assess the relationship between this specific single nucleotide polymorphism (SNP) and the risk of developing tuberculosis. A systematic literature search was performed utilizing the electronic databases PubMed, Web of Science, Scopus, and ISI to capture all relevant publications available through April 2024. To ensure comprehensive coverage, the reference lists of retrieved full-text articles were also manually scrutinized. For the quantitative synthesis, pooled odds ratios (ORs) with their corresponding 95% confidence intervals (CIs) were computed to determine the overall effect estimates. The chi-square (χ²) test and the I² statistic were applied to assess and quantify heterogeneity. This meta-analysis included 6363 individuals (2904 TB patients and 3459 healthy controls) from eight case-control studies. The pooled effect estimates across all genetic inheritance models (e.g., dominant model: OR = 0.971, 95% CI: 0.884-1.068) did not reveal a significant link between the rs2910164 polymorphism and susceptibility to tuberculosis. The analysis revealed considerable heterogeneity across most genetic models, as indicated by I² statistics exceeding 70%. Conversely, statistical tests found no evidence of publication bias. The collective evidence from this analysis does not support a significant association between the <i>miR-146a</i> rs2910164 G>C variant and tuberculosis susceptibility. Confirmation of this null association necessitates future validation in large-scale, rigorously designed studies.</p>","PeriodicalId":14152,"journal":{"name":"International Journal of Molecular and Cellular Medicine","volume":"14 4","pages":"1084-1098"},"PeriodicalIF":1.8,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13092839/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147770874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated Bioinformatics and Experimental Validation of the hsa_circ_0000378/miR-205-5p/RAD51 ceRNA Axis in Breast Cancer.","authors":"Ali Abbasi, Nahid Nafisi, Pejman Morovat, Mitra Nourbakhsh, Mehdi Sepidarkish, Mohamadreza Ahmadifard, Arash M Ashrafi, Mehdi Pouramir","doi":"10.22088/IJMCM.BUMS.14.4.994","DOIUrl":"10.22088/IJMCM.BUMS.14.4.994","url":null,"abstract":"<p><p>Breast cancer (BC) remains the leading cause of cancer-related mortality in women worldwide, primarily due to its high invasiveness and therapeutic resistance. This study explores the role of noncoding RNAs, circular RNAs (circRNAs), in BC progression through a competing endogenous RNA (ceRNA) network. Three GEO circRNA microarray datasets (GSE101123, GSE165884, GSE182471) were retrieved, normalized, and batch-corrected using ComBat. Differentially expressed circRNAs (DEcircRNAs) were identified via limma (|log₂FC| > 1, FDR < 0.05). Differentially expressed miRNAs (DEmiRNAs) and mRNAs (DEgenes) were derived from TCGA-BRCA RNA-Seq (1,091 tumors, 113 normals) and miRNA-Seq (1,078 tumors, 104 normals) data using DESeq2 (|log₂FC| > 1, FDR < 0.05). CircRNAs harboring miRNA response elements (MREs) were selected via CSCD, and miRNA-mRNA interactions predicted through TarBase, prioritizing upregulated DEgenes. A ceRNA network was constructed in Cytoscape based on expression concordance. The hsa_circ_0000378/hsa-miR-205-5p/RAD51 axis was validated in 48 paired BC and adjacent non-tumor tissues by RT-qPCR. Results indicated hsa_circ_0000378 upregulation (2.74-fold, p<0.001), hsa-miR-205-5p downregulation (0.64-fold, p=0.0022), and RAD51 upregulation (3.46-fold, p<0.001) in tumors. Spearman correlations showed negative associations between hsa_circ_0000378 and hsa-miR-205-5p (r = -0.474, p<0.001), hsa-miR-205-5p and RAD51 (r = -0.383, p<0.001), and positive between hsa_circ_0000378 and RAD51 (r = 0.497, p<0.001), supporting ceRNA regulation. ROC analysis revealed RAD51's diagnostic potential (AUC=0.83, 95% CI: 0.74-0.90, sensitivity=0.81, specificity=0.55), followed by hsa_circ_0000378 (AUC=0.75, 95% CI: 0.65-0.85, sensitivity=0.71, specificity=0.77), and hsa-miR-205-5p (AUC=0.66, 95% CI: 0.56-0.76, sensitivity=0.69, specificity=0.55). These results propose the hsa_circ_0000378/hsa_miR-205-5p/RAD51 axis as a potential biomarker; mechanistic validation and larger cohorts are needed for clinical application.</p>","PeriodicalId":14152,"journal":{"name":"International Journal of Molecular and Cellular Medicine","volume":"14 4","pages":"994-1013"},"PeriodicalIF":1.8,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13092840/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147771018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}