International Journal of Molecular and Cellular Medicine最新文献_第10页

Applying Vasopressin-Pre-Conditioned Human Adipose Mesenchymal Stem Cells Improves Heart Condition after Transplantation into Infarcted Myocardium. 应用加压素预处理的人脂肪间充质干细胞改善梗死心肌移植后的心脏状况。

International Journal of Molecular and Cellular Medicine Pub Date : 2022-01-01 DOI: 10.22088/IJMCM.BUMS.11.3.207

Shakiba Nasiri Boroujeni, Farzaneh Chehelcheraghi, Mojtaba Khaksarian, Mehrnoosh Sedighi, Vajihe Ghorbanzadeh, Afshin Nazari

{"title":"Applying Vasopressin-Pre-Conditioned Human Adipose Mesenchymal Stem Cells Improves Heart Condition after Transplantation into Infarcted Myocardium.","authors":"Shakiba Nasiri Boroujeni, Farzaneh Chehelcheraghi, Mojtaba Khaksarian, Mehrnoosh Sedighi, Vajihe Ghorbanzadeh, Afshin Nazari","doi":"10.22088/IJMCM.BUMS.11.3.207","DOIUrl":"https://doi.org/10.22088/IJMCM.BUMS.11.3.207","url":null,"abstract":"Transplantation of H-AdMSCs may improve heart function after MI. AVP is a neurohypophyseal hormone that reduces cardiovascular damage. This study investigated the role of AVP preconditioning in the survival of MSCs and their effect on myocardial repair in the MI rats. H-AMSCs were isolated and incubated for 3 days. The expression of oxytocin and vasopressin receptors was evaluated by Real-time-PCR. Forty male Wistar rats were divided into 4 groups: control, sham, ASC and AVP-ASC. Ischemia was established by ligation of LAD coronary artery. Electrocardiography, fibrosis, angiogenesis, and apoptosis in myocardium were determined after 7 days. Results showed that preconditioned MSCs signiﬁcantly increased cardiac function when compared with group that received non-preconditioned MSCs. This was associated with signiﬁcantly reduced ﬁbrosis, increased vascular density, and decreased resident myocyte apoptosis. Results indicate that AVP preconditioned MSCs can be consider a novel approach to management of MI.","PeriodicalId":14152,"journal":{"name":"International Journal of Molecular and Cellular Medicine","volume":"11 3","pages":"207-222"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/5c/40/ijmcm-11-207.PMC10440004.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10425852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual Effects of Resveratrol on the Expression and Secretion of Angiogenic Factors. 白藜芦醇对血管生成因子表达和分泌的双重影响。

International Journal of Molecular and Cellular Medicine Pub Date : 2022-01-01 Epub Date: 2022-10-03 DOI: 10.22088/IJMCM.BUMS.11.1.16

Pegah Kiamehr, Minoo Shahidi, Amir Samii, Farhad Zaker

{"title":"Dual Effects of Resveratrol on the Expression and Secretion of Angiogenic Factors.","authors":"Pegah Kiamehr, Minoo Shahidi, Amir Samii, Farhad Zaker","doi":"10.22088/IJMCM.BUMS.11.1.16","DOIUrl":"https://doi.org/10.22088/IJMCM.BUMS.11.1.16","url":null,"abstract":"Angiogenesis is an essential process in the growth, development, and transition of tumors from dormancy to proliferating state. Resveratrol (RSV), as a natural polyphenolic compound, is claimed to be effective in regulating angiogenesis. This study aimed to evaluate the impact of RSV onthe angiogenesis process in HUVECs (human umbilical vein endothelial cells) alone and co-cultured with Jurkat cells. The effects of RSV on HUVECs and Jurkat cell viability and apoptosis were measured by MTT and Annexin-V/PI methods. HUVECs were co-cultured with pre-treated Jurkat cells and incubated for 24 h, 48 h and 72 h. The angiogenesis process in HUVECs and Jurkat cells alone and in co-culture models was investigated by analyzing the expression of VEGF, VEGFR-2, and Interleukin-8 (IL-8) employing qPCR and ELISA. RSV at low concentration (40 µM) had no significant effects on apoptosis rate of HUVECs, but higher concentrations (80-160 µM) increased apoptosis in co-culture method and HUVECs alone. RSV significantly reduced VEGFR2 and IL-8 gene expression also, IL-8 protein concentration in HUVECs, but the effects of this drug in the HUVECs-Jurkats co-culture were different. Expression of VEGF in Jurkat cells increased following treatment with RSV. RSV had direct anti-angiogenic effects on HUVECs. Unexpectedly its indirect effects were not significant on HUVECs-Jurkats co-culture. Results of our study showed, RSV may be effective in anti-angiogenesis therapy, but in some situations, it may induce angiogenesis. So, appropriate concentrations should achieve to minimize the unpredicted effects of RSV","PeriodicalId":14152,"journal":{"name":"International Journal of Molecular and Cellular Medicine","volume":"11 1","pages":"16-30"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/c5/5b/ijmcm-11-16.PMC9653554.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40695714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Upregulation of Neurotrophic Factors and Myelin Basic Protein in Schwann-like Cells by T3 Hormone Following Transdifferentiation of Human Adipose-derived Stem Cells. 人脂肪干细胞转分化后T3激素对雪旺样细胞中神经营养因子和髓鞘碱性蛋白的上调

International Journal of Molecular and Cellular Medicine Pub Date : 2022-01-01 Epub Date: 2022-10-03 DOI: 10.22088/IJMCM.BUMS.11.1.41

Giti Zarinfard, Maryam Aliakbari, Vajihe Asgari, Shahnaz Razavi

{"title":"Upregulation of Neurotrophic Factors and Myelin Basic Protein in Schwann-like Cells by T3 Hormone Following Transdifferentiation of Human Adipose-derived Stem Cells.","authors":"Giti Zarinfard, Maryam Aliakbari, Vajihe Asgari, Shahnaz Razavi","doi":"10.22088/IJMCM.BUMS.11.1.41","DOIUrl":"https://doi.org/10.22088/IJMCM.BUMS.11.1.41","url":null,"abstract":"Peripheral nerve regeneration is a complicated phenomenon. Thyroid hormones are known as critical regulators in the nervous system development. The Schwann cells have the regenerative potency in the peripheral nervous system. In this study, the human adipose-derived stem cells were assessed in vitro, for transdifferentiation potency into Shwann-like cells (SLCs) as a candidate source for clinical cell therapy, under the treatment of triiodothyronine (T3) hormone, and compared with the untreated cells. The cell viability rate, myelination and neurotrophic factors expression of SLCs were evaluated two weeks post- induction by MTT assay, immunocytochemistry and real-time RT-PCR techniques, respectively. The obtained results revealed a significant decrease in SLCs viability, compared to the adipose-derived stem cells (P < 0.001). Immunocytochemistry technique was applied to detect SLCs markers, such as S100β, GFAP and myelin basic proteins (MBP) in the presence and absence of T3 treatment. The results indicated that administering T3 can significantly increase the differentiation and myelination potency of SLCs (P < 0.01). The findings of real-time RT-PCR technique indicated that the expression of Schwann cells markers, MBP, brain-derived neurotrophic factor and glial cell-derived neurotrophic factor were upregulated significantly with T3 hormone administration in comparison with the untreated cells (P < 0.05). The SLCs were able to express the neurotrophic factors and myelination related genes in the presence of T3 hormone. Furthermore, T3 administration improved myelination potency of adipose-derived stem cells, in vitro. Further in vivo experiments are necessary to confirm the advantages of using a combination of autologous SLCs and T3 hormone for peripheral nerve injury recovery.","PeriodicalId":14152,"journal":{"name":"International Journal of Molecular and Cellular Medicine","volume":"11 1","pages":"41-54"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/03/64/ijmcm-11-41.PMC9653553.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40695715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Prevalence of Co-infection by Human Papillomavirus, Epstein- Barr Virus and Merkel Cell Polyomavirus in Iranian Oral Cavity Cancer and Pre-malignant Lesions. 伊朗口腔癌和恶性前病变中人类乳头瘤病毒、爱泼斯坦-巴氏病毒和梅克尔细胞多瘤病毒合并感染的流行率

IF 1.5

International Journal of Molecular and Cellular Medicine Pub Date : 2022-01-01 Epub Date: 2022-10-03 DOI: 10.22088/IJMCM.BUMS.11.1.64

Sagahr Saber Amoli, Ali Hasanzadeh, Farzin Sadeghi, Mohammad Chehrazi, Maryam Seyedmajidi, Arghavan Zebardast, Yousef Yahyapour

{"title":"Prevalence of Co-infection by Human Papillomavirus, Epstein- Barr Virus and Merkel Cell Polyomavirus in Iranian Oral Cavity Cancer and Pre-malignant Lesions.","authors":"Sagahr Saber Amoli, Ali Hasanzadeh, Farzin Sadeghi, Mohammad Chehrazi, Maryam Seyedmajidi, Arghavan Zebardast, Yousef Yahyapour","doi":"10.22088/IJMCM.BUMS.11.1.64","DOIUrl":"10.22088/IJMCM.BUMS.11.1.64","url":null,"abstract":"Human papillomavirus (HPV) is recognized as the most important risk factor in oral cavity cancer and pre-malignant lesions; however, the etiological association of concomitant infection with other oncogenic viruses as a co-factor has not been definitively proven. The present study aimed to determine the prevalence of co-infection with HPV, Epstein-Barr virus (EBV) and Merkel Cell PolyomaVirus (MCPyV) in oral cavity lesions in Iranian patients. One hundred and fourteen oral cavity samples, including 33 oral squamous cell carcinoma, 28 oral lichen planus, 16 oral epithelial dysplasia and 37 oral irritation fibromas were analyzed for the HPV, EBV and MCPyV infection by quantitative real-time PCR. According to histological features 32.5% and 28.9% of cases were oral irritation fibroma and oral squamous cell carcinoma, respectively. Infection with at least two viruses was detected in 21.1% of patients. In this group, co-infection with HPV/EBV was identified in 37.5% of cases, HPV/MCPyV in 29.2%, EBV/MCPyV in 12.5%, and HPV/EBV/MCPyV in 20.8%. There was no statistically significant difference between multiple infections and anatomical locations of cancer. The prevalence of triple viral infection (HPV/EBV/MCPyV) in well differentiated tumors was higher than EBV or MCPyV single infection. This study revealed that co-infection of HPV, EBV and MCPyV can be detected in both malignant and non-malignant oral cavity tissues, and co-infection with all three viruses in well differentiated tumors can be shown as a synergistic hypothesis of the pathogenic role of these viruses in oral malignant transformation.","PeriodicalId":14152,"journal":{"name":"International Journal of Molecular and Cellular Medicine","volume":"11 1","pages":"64-77"},"PeriodicalIF":1.5,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a3/43/ijmcm-11-64.PMC9653548.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40695716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Increased Expression of Tight Junction Proteins and Blood-Brain Barrier Integrity in MCAO Rats Following Injection of miR-149-5p. 注射miR-149-5p后MCAO大鼠紧密连接蛋白和血脑屏障完整性的表达增加。

International Journal of Molecular and Cellular Medicine Pub Date : 2022-01-01 DOI: 10.22088/IJMCM.BUMS.11.3.223

Meysam Forouzandeh, Hossein Mostafavi, Elham Ghasemloo, Parvin Mohammadi, Masoume Hosseini, Mehdi Eskandari

{"title":"Increased Expression of Tight Junction Proteins and Blood-Brain Barrier Integrity in MCAO Rats Following Injection of miR-149-5p.","authors":"Meysam Forouzandeh, Hossein Mostafavi, Elham Ghasemloo, Parvin Mohammadi, Masoume Hosseini, Mehdi Eskandari","doi":"10.22088/IJMCM.BUMS.11.3.223","DOIUrl":"https://doi.org/10.22088/IJMCM.BUMS.11.3.223","url":null,"abstract":"Cerebral ischemia is a common neurodegenerative disease in which damage to the blood-brain barrier (BBB) is the main consequence. In cerebral ischemia, the level of miR-149-5p and tight junction proteins are decreased, while the level of Calpine is increased, finally leading to increased BBB permeability. This study investigated the effect of miR-149-5p mimic on the expression of Calpain, Occludin, and ZO-1 and the consequences of cerebral ischemia. Cerebral ischemia model was performed via middle cerebral artery occlusion (MCAO) method on female Wistar rats. Four groups of Wistar rats were studied: Sham, cerebral ischemia without treatment, Scramble miR, and miR-149-5p mimic treatment. Then, neurological defects and BBB permeability (via Evans blue staining), cerebral edema (cerebrospinal fluid percentage), and ZO-1, Occludin, and Calapin expression (by quantitative real time- PCR) were investigated. qRT-PCR results showed miR-149-5p expression decreases after cerebral ischemia induction. In addition, Occludin and ZO-1 expression significantly increased in miR-149-5p group. In contrast, Calapin expression, BBB permeability, brain water content and neurological defects were significantly decreased. It seems that the increased level of miR-149-5p exerts its protective effect on cerebral ischemia due to increasing of tight junction proteins.","PeriodicalId":14152,"journal":{"name":"International Journal of Molecular and Cellular Medicine","volume":"11 3","pages":"223-235"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/77/77/ijmcm-11-223.PMC10440002.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10406762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel Variant Identified in the Enhancer Region of Host Transcription Factor, BRN3A, is a Significant Risk Factor for HPV-Induced Uterine Cervix Cancer. 宿主转录因子增强子区的新变异BRN3A是hpv诱发宫颈癌的重要危险因素。

International Journal of Molecular and Cellular Medicine Pub Date : 2022-01-01 DOI: 10.22088/IJMCM.BUMS.11.2.88

Anand Prakash, Biswa Pratim Das Purkayastha, Shikha Srivastava, Sunanda Chaturvedi, Akhtar Ali, Dau Dayal Aggarwal, Jagat Kumar Roy

{"title":"Novel Variant Identified in the Enhancer Region of Host Transcription Factor, BRN3A, is a Significant Risk Factor for HPV-Induced Uterine Cervix Cancer.","authors":"Anand Prakash, Biswa Pratim Das Purkayastha, Shikha Srivastava, Sunanda Chaturvedi, Akhtar Ali, Dau Dayal Aggarwal, Jagat Kumar Roy","doi":"10.22088/IJMCM.BUMS.11.2.88","DOIUrl":"https://doi.org/10.22088/IJMCM.BUMS.11.2.88","url":null,"abstract":"Among the HPV-mediated cervical cancers, cellular factor BRN3A has gained considerable attention due to its role in promoting an anti-apoptotic cellular environment and in facilitating epitheliotropic transformations of the host. The majority of previous studies looked at BRN3A's molecular characteristics; however, the possibility of genetic variations in BRN3A's auto-regulatory region in relation to cervical cancer risk has been underestimated until now. In a retrospective study in the Eastern UP population, India, we detected genetic variations in the cis-regulatory proximal enhancer region located around 5.6 kb upstream of transcription start site of BRN3A. Our analysis of PCR and DNA sequencing confirmed this novel SNP (BRN3A g.60163379A>G) within the auto-regulatory region of BRN3A. As compared to control subjects, cancer cases exhibited a 1.32-fold higher allele frequency (χ2 = 6.315, p = 0.012). In homozygous (GG) but not in heterozygous conditions, odds ratio (OR) analysis suggests a significant association of cancer risk with the SNP (OR = 2.60, p ≤ 0.004). We further confirmed using the functional analysis that this SNP increased the luciferase gene activity in HPV-positive cervical cancer SiHa cells that were exposed to progesterone. As a result of the association of polymorphisms in a non-coding region of an oncogene with increased cancer risks, we are suggesting that this genetic variation in non-coding region can be used in prediction, diagnosis, or predicting the progression of the disease.","PeriodicalId":14152,"journal":{"name":"International Journal of Molecular and Cellular Medicine","volume":"11 2","pages":"88-103"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/03/68/ijmcm-11-088.PMC10116355.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9388148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nrf2 rs6721961 and Oxidative Stress in Preeclampsia: Association with the Risk of Preeclampsia and Early-Onset Preeclampsia. Nrf2 rs6721961和氧化应激在子痫前期:与子痫前期和早发性子痫前期的风险相关

International Journal of Molecular and Cellular Medicine Pub Date : 2022-01-01 DOI: 10.22088/IJMCM.BUMS.11.2.127

Fatemeh Khadir, Zohreh Rahimi, Azita Ghanbarpour, Asad Vaisi-Raygani

{"title":"Nrf2 rs6721961 and Oxidative Stress in Preeclampsia: Association with the Risk of Preeclampsia and Early-Onset Preeclampsia.","authors":"Fatemeh Khadir, Zohreh Rahimi, Azita Ghanbarpour, Asad Vaisi-Raygani","doi":"10.22088/IJMCM.BUMS.11.2.127","DOIUrl":"https://doi.org/10.22088/IJMCM.BUMS.11.2.127","url":null,"abstract":"Preeclampsia as a multifactor hypertensive disorder of pregnancy is associated with enhanced placental oxidative stress. The Keap1-Nrf2 pathway protects cells against oxidative stress. We examined the possible association between the Nrf2 variants in relation to oxidative stress parameters with the risk of preeclampsia. We studied 150 preeclampsia women and 150 women with a normal pregnancy to find the frequency of Nrf2 rs6721961 genotypes using the PCR-RFLP method. Also, an association between the Nrf2 genotypes with the levels of malondialdehyde (MDA) and total antioxidant capacity (TAC) was analyzed. Significantly lower TAC and higher MDA levels were found in preeclampsia patients compared to controls (P<0.0001). For the first time, we report an association between the Nrf2 rs6721961 polymorphism and preeclampsia risk. The present study indicated that the GT genotype and the T allele of the Nrf2 rs6721961 increased the risk of preeclampsia by 2.81 and 2.39 times, respectively. Also, the Nrf2 TT genotype was associated with a 3.9-fold increased risk of early-onset preeclampsia. We detected a positive association between the levels of body mass index, MDA, and the Nrf2 polymorphism with the risk of preeclampsia and a negative correlation between the level of TAC with the preeclampsia risk. Also, an association between the rs6721961 TT genotype with higher serum MDA levels was found. Our study suggests oxidative stress is involved in the pathogenesis of preeclampsia and the Nrf2 rs6721961 polymorphism through alteration in the levels of oxidative stress parameters might increase the risk of preeclampsia and early-onset preeclampsia.","PeriodicalId":14152,"journal":{"name":"International Journal of Molecular and Cellular Medicine","volume":"11 2","pages":"127-136"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/df/28/ijmcm-11-127.PMC10116352.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9388150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

hsa-miR-508-5p as a New Potential Player in Intervertebral Disc Degeneration. hsa-miR-508-5p作为椎间盘退变的新潜在参与者。

International Journal of Molecular and Cellular Medicine Pub Date : 2022-01-01 DOI: 10.22088/IJMCM.BUMS.11.2.137

Akram Gholipour, Mahshid Malakootian, Maziar Oveisee

{"title":"hsa-miR-508-5p as a New Potential Player in Intervertebral Disc Degeneration.","authors":"Akram Gholipour, Mahshid Malakootian, Maziar Oveisee","doi":"10.22088/IJMCM.BUMS.11.2.137","DOIUrl":"https://doi.org/10.22088/IJMCM.BUMS.11.2.137","url":null,"abstract":"Intervertebral disc degeneration (IDD) is widely known as the principal cause of low back pain, diminishing patients' quality of life and imposing a huge economic burden on healthcare systems worldwide. However, the underlying mechanisms of IDD remain to be determined. This study aimed to scrutinize data sets via bioinformatics to identify microRNAs (miRNAs)/genes and pathways associated with IDD. The array profiling of patients with IDD and individuals without IDD was acquired from the Gene Expression Omnibus (GEO) database (viz., GSE19943, GSE63492, and GSE34095). The expression profiles of miRNAs and genes with differential patterns were analyzed using GEO2R. The target genes of the chosen miRNA were then examined, and in silico functional analyses were performed on the signaling pathways and biological processes of the differentially expressed genes. Three human miRNAs were up and downregulated in IDD patients in the examined data sets. Among them, hsa-miR-508-5p had a significant differential expression in the IDD group, and SEC11A, IPO5, FN1, and MRPS10, as the targets of hsa-miR-508-5p, were upregulated in the IDD group. Furthermore, extracellular matrix-receptor interactions, focal adhesion, and actin cytoskeleton regulation were important pathways involved in IDD. Our analysis identified hsa-miR-508-5p as a novel miRNA involved in IDD pathogenies. Our findings not only further confirmed the significant role of miRNAs in IDD pathogenesis but also extended the spectrum of the miRNAs and genes involved in IDD. Though, still, further experimental investigations are needed to confirm our findings.","PeriodicalId":14152,"journal":{"name":"International Journal of Molecular and Cellular Medicine","volume":"11 2","pages":"137-149"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/46/67/ijmcm-11-137.PMC10116350.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9388153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Sesamin Acts as Anti-leukemic Compound Interacting with Novel Phosphoprotein Targets and Inducing Apoptosis in Leukemic Cells. 芝麻素作为抗白血病化合物与新的磷酸化蛋白靶点相互作用并诱导白血病细胞凋亡。

International Journal of Molecular and Cellular Medicine Pub Date : 2022-01-01 Epub Date: 2022-10-04 DOI: 10.22088/IJMCM.BUMS.11.1.1

Pattharin Wannapruk, Kamolchanok Deesrisak, Sittiruk Roytrakul, Dalina Tanyong

{"title":"Sesamin Acts as Anti-leukemic Compound Interacting with Novel Phosphoprotein Targets and Inducing Apoptosis in Leukemic Cells.","authors":"Pattharin Wannapruk, Kamolchanok Deesrisak, Sittiruk Roytrakul, Dalina Tanyong","doi":"10.22088/IJMCM.BUMS.11.1.1","DOIUrl":"https://doi.org/10.22088/IJMCM.BUMS.11.1.1","url":null,"abstract":"Leukemia is one of the high-incidence cancers that is characterized by an abnormal production of immature white blood cells. Subject to many reports on the side effects of conventional chemotherapy, herbs and natural compounds have been studied as an alternative medicine. In this study, sesamin, a lignan in sesame seed with pharmaceutical functions including anti-cancer, was chosen and treated with MOLT-4 and NB4 leukemic cell lines in various concentrations for 24 and 48 hours. The effect of sesamin on cell inhibition and expression levels of apoptotic genes in leukemic cell lines were investigated by MTT assay and real-time PCR, respectively. Moreover, apoptotic proteins were studied by mass spectrometry and bioinformatics tools to investigate the relation between sesamin and targeted proteins. Results showed that sesamin increased cell inhibition in both cell lines in dose- and time-dependent manner. Levels of caspase-3, -7, -8, and -9 gene expressions significantly increased, while BCL-2 decreased drastically in sesamin-treated cells. From bioinformatics study, PARP4, IPPK and caspase family proteins were found to be involved in sesamin that induced apoptosis in leukemic cells. Besides, doxorubicin, a chemotherapeutic drug, also shared the same protein targets as sesamin in apoptosis pathway. Sesamin demonstrates its potential to enhance cell inhibition and promotes cell apoptosis in both MOLT-4 and NB4 leukemic cell lines. This study will benefit the development of sesamin as an effective anti-leukemia drug in the future.","PeriodicalId":14152,"journal":{"name":"International Journal of Molecular and Cellular Medicine","volume":"11 1","pages":"1-15"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/26/0e/ijmcm-11-1.PMC9653549.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40695718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring VEGF-Linked Pathways: Investigating Multiple miRNAs for Their Therapeutic Potential in Angiogenesis Targets and as Biomarkers in Recurrent Glioblastoma Multiforme. 探索VEGF相关通路：研究多种miRNA在血管生成靶点中的治疗潜力以及作为复发性多型胶质母细胞瘤的生物标志物。

International Journal of Molecular and Cellular Medicine Pub Date : 2022-01-01 DOI: 10.22088/IJMCM.BUMS.11.4.306

Morteza Hadizadeh, Ramin Soltani, Taimour Langaee, Marziye Shadpirouz, Sorayya Ghasemi

{"title":"Exploring VEGF-Linked Pathways: Investigating Multiple miRNAs for Their Therapeutic Potential in Angiogenesis Targets and as Biomarkers in Recurrent Glioblastoma Multiforme.","authors":"Morteza Hadizadeh, Ramin Soltani, Taimour Langaee, Marziye Shadpirouz, Sorayya Ghasemi","doi":"10.22088/IJMCM.BUMS.11.4.306","DOIUrl":"https://doi.org/10.22088/IJMCM.BUMS.11.4.306","url":null,"abstract":"Alternative pathways frequently operate as the origins of resistance to drugs that block the vascular endothelial growth factor (VEGF) pathway. To find possible therapeutic targets and indicators, this study explored the VEGF pathway and how miRNAs control it in recurrent glioblastoma multiforme (rGBM). Differentially expressed miRNAs (DEmiRNAs) were identified by using GBM GSE profiles (GSE32466). To find pathways containing DEmiRNAs, VEGF pathway genes, and their related genes, DIANA-miRPath v3.0 and the ToppGene database were utilized. miRNAs linked to VEGF signaling pathway genes, interactional genes, and DEmiRNAs were discovered by extracting common pathways. The ability of these miRNAs to distinguish rGBM patients from those with primary GBM was assessed using ROC analysis. The study revealed that in rGBM, 30 miRNAs were significantly up-regulated and 49 miRNAs were considerably down-regulated. Among them, the VEGF pathway was connected to 22 up-regulated miRNAs and 29 down-regulated miRNAs. The MAPK pathway shared the most genes with the VEGF pathway, accounting for 1,014 of the interacting genes, which were discovered to have interactions with VEGF signaling pathway genes. Furthermore, 14 miRNAs were identified as having a great deal of potential as molecular biomarkers and therapeutic targets for rGBM. The results indicate that the VEGF pathway in rGBM is regulated by a number of interrelated pathways. The discovered miRNAs hold promise as rGBM biomarkers and therapeutic targets, offering possibilities for novel therapy strategies and aiding rGBM diagnosis and prognosis.","PeriodicalId":14152,"journal":{"name":"International Journal of Molecular and Cellular Medicine","volume":"11 4","pages":"306-319"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/fc/af/ijmcm-11-306.PMC10506677.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41119811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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