Dual Effects of Resveratrol on the Expression and Secretion of Angiogenic Factors.

IF 1.5 Q3 MEDICINE, RESEARCH & EXPERIMENTAL
Pegah Kiamehr, Minoo Shahidi, Amir Samii, Farhad Zaker
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引用次数: 1

Abstract

Angiogenesis is an essential process in the growth, development, and transition of tumors from dormancy to proliferating state. Resveratrol (RSV), as a natural polyphenolic compound, is claimed to be effective in regulating angiogenesis. This study aimed to evaluate the impact of RSV onthe angiogenesis process in HUVECs (human umbilical vein endothelial cells) alone and co-cultured with Jurkat cells. The effects of RSV on HUVECs and Jurkat cell viability and apoptosis were measured by MTT and Annexin-V/PI methods. HUVECs were co-cultured with pre-treated Jurkat cells and incubated for 24 h, 48 h and 72 h. The angiogenesis process in HUVECs and Jurkat cells alone and in co-culture models was investigated by analyzing the expression of VEGF, VEGFR-2, and Interleukin-8 (IL-8) employing qPCR and ELISA. RSV at low concentration (40 µM) had no significant effects on apoptosis rate of HUVECs, but higher concentrations (80-160 µM) increased apoptosis in co-culture method and HUVECs alone. RSV significantly reduced VEGFR2 and IL-8 gene expression also, IL-8 protein concentration in HUVECs, but the effects of this drug in the HUVECs-Jurkats co-culture were different. Expression of VEGF in Jurkat cells increased following treatment with RSV. RSV had direct anti-angiogenic effects on HUVECs. Unexpectedly its indirect effects were not significant on HUVECs-Jurkats co-culture. Results of our study showed, RSV may be effective in anti-angiogenesis therapy, but in some situations, it may induce angiogenesis. So, appropriate concentrations should achieve to minimize the unpredicted effects of RSV

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白藜芦醇对血管生成因子表达和分泌的双重影响。
血管生成是肿瘤从休眠状态向增殖状态转变的重要过程。白藜芦醇(Resveratrol, RSV)是一种天然多酚类化合物,具有调节血管生成的作用。本研究旨在评估RSV对人脐静脉内皮细胞(HUVECs)单独和与Jurkat细胞共培养血管生成过程的影响。采用MTT法和Annexin-V/PI法检测RSV对HUVECs和Jurkat细胞活力和凋亡的影响。将HUVECs与预处理过的Jurkat细胞共培养24 h、48 h和72 h,采用qPCR和ELISA分析VEGF、VEGFR-2和白细胞介素-8 (IL-8)的表达,研究HUVECs与Jurkat细胞单独和共培养模型的血管生成过程。低浓度(40µM) RSV对HUVECs的凋亡率无显著影响,而高浓度(80 ~ 160µM) RSV可促进共培养法和单独培养HUVECs的细胞凋亡。RSV显著降低了HUVECs中VEGFR2和IL-8基因的表达以及IL-8蛋白的浓度,但该药在HUVECs- jurkats共培养中的作用不同。RSV处理后Jurkat细胞中VEGF表达增加。RSV对HUVECs有直接的抗血管生成作用。出乎意料的是,它对huvec - jurkats共培养的间接影响并不显著。我们的研究结果表明,RSV可能在抗血管生成治疗中有效,但在某些情况下,它可能会诱导血管生成。因此,应达到适当的浓度,以尽量减少RSV的不可预测的影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
3.60
自引率
0.00%
发文量
0
期刊介绍: The International Journal of Molecular and Cellular Medicine (IJMCM) is a peer-reviewed, quarterly publication of Cellular and Molecular Biology Research Center (CMBRC), Babol University of Medical Sciences, Babol, Iran. The journal covers all cellular & molecular biology and medicine disciplines such as the genetic basis of disease, biomarker discovery in diagnosis and treatment, genomics and proteomics, bioinformatics, computer applications in human biology, stem cells and tissue engineering, medical biotechnology, nanomedicine, cellular processes related to growth, death and survival, clinical biochemistry, molecular & cellular immunology, molecular and cellular aspects of infectious disease and cancer research. IJMCM is a free access journal. All open access articles published in IJMCM are distributed under the terms of the Creative Commons Attribution CC BY. The journal doesn''t have any submission and article processing charges (APCs).
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