International Journal of Molecular and Cellular Medicine最新文献

{"title":"Caffeine Modulates Cell Death and Telomerase Activity in Triple-negative Breast Cancer Cells.","authors":"Qanita Hana Amira, Shadira Anindieta Irdianto, Fadilah Fadilah, Retno Lestari, Fadhillah Fadhillah, Anom Bowolaksono, Astari Dwiranti","doi":"10.22088/IJMCM.BUMS.14.2.606","DOIUrl":"10.22088/IJMCM.BUMS.14.2.606","url":null,"abstract":"<p><p>Triple-negative breast cancer (TNBC) is an aggressive subtype defined by the lack of estrogen receptors (ER), progesterone receptors (PR), and HER2 expression, resulting in limited therapeutic options. Given this challenge, this study explores caffeine, a widely consumed stimulant, as a potential anticancer agent, particularly for TNBC. Although caffeine has demonstrated stimulatory and inhibitory effects on telomerase in other cancer types, its influence on telomerase activity in TNBC remains uncharacterized. This study investigates the impact of caffeine concentrations (10, 15, and 20 mM) on cell viability, proliferation, apoptosis, ultrastructure, and the expression of apoptosis-related genes (<i>BAX, BCL2, CASP8</i>) and telomerase activity (<i>hTERT</i>) in MDA-MB-231 cells. Our findings showed that caffeine significantly reduces cell viability and induces early apoptosis with a dose-dependent effect. Morphological changes consistent with early apoptosis were observed, and an increased <i>BAX/BCL2</i> ratio indicated the activation of the intrinsic apoptosis pathway. Additionally, caffeine exhibited upregulation of <i>hTERT</i> mRNA expression, which may reflect a compensatory response to cellular stress induced by caffeine. These results underscore the multifaceted effects of caffeine on TNBC cells, highlighting its potential not only as an apoptosis inducer but also as a modulator of telomerase activity. Given its accessibility, low toxicity, and established safety profile, caffeine presents an exciting avenue for further research as a complementary or standalone therapeutic strategy for TNBC.</p>","PeriodicalId":14152,"journal":{"name":"International Journal of Molecular and Cellular Medicine","volume":"14 2","pages":"606-619"},"PeriodicalIF":0.8,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12320964/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144789070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral squamous cell carcinoma pharmacological treatment; A long non-coding RNAs (long ncRNAs) story.","authors":"Azita Sadeghzade, Ali Jafarian, Amir Hossein Davodpour, Mohsen Pouresmaeliyan Roumani, Meysam Mohammadikhah, Sarah Qutaiba Badraldeen, Sajad Ataei Azimi","doi":"10.22088/IJMCM.BUMS.14.2.736","DOIUrl":"10.22088/IJMCM.BUMS.14.2.736","url":null,"abstract":"<p><p>Oral squamous cell cancer (OSCC) is a major global health issue, ranking sixth in prevalence, particularly in Asia. The diagnosis often occurs late due to inadequate early screening, resulting in a dismal five-year survival rate of around 50%. This document provides a comprehensive analysis of drug-based treatments for oral cavity carcinoma, focusing on chemotherapy, immune modulation, and novel approaches like nanoparticle therapies. Despite advancements in these methods, drug resistance remains a significant obstacle that adversely affects patient outcomes. The research highlights the critical role of long ncRNAs in the progression and treatment of OSCC. These long ncRNAs, which are over 200 nucleotides long, play essential roles in gene regulation and tumor growth, including mechanisms of drug resistance. Some long ncRNAs may promote or inhibit tumor development and influence the effectiveness of anti-cancer drugs like cisplatin. Additionally, the review explores how the tumor microenvironment and immune responses interact, suggesting that inflammation may accelerate the progression of oral cancer. By synthesizing insights from extensive literature, this review clarifies the complex relationship between long ncRNAs and OSCC treatment. The study aimed to improve treatment efficacy and increase survival rates for patients with oral squamous cell carcinoma by identifying potential therapeutic targets. The findings underscore the importance of integrating molecular insights into treatment strategies to combat drug resistance and enhance patient outcomes in oral cancer therapy.</p>","PeriodicalId":14152,"journal":{"name":"International Journal of Molecular and Cellular Medicine","volume":"14 2","pages":"736-752"},"PeriodicalIF":0.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12321307/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144789041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing Production Conditions of a Caspian Sea <i>Actinomycete</i> Exhibiting Promising Antibacterial Activity Against Clinically-important Pathogens Using the Two-Factor Interaction/ Minimum Run Resolution IV Method.","authors":"Zeinab Jan, Hamed Norouzi Taheri, Abolghasem Danesh","doi":"10.22088/IJMCM.BUMS.14.2.666","DOIUrl":"10.22088/IJMCM.BUMS.14.2.666","url":null,"abstract":"<p><p>Natural products recovered from marine sediment have the potential for the treatment of various diseases. <i>Streptomyces</i> sp., strain MN38 which was previously isolated from the Caspian Sea of Iran was at first characterized based on its 16srRNA analysis and morphological properties. Two-factor Interaction/ Minimum Run Resolution IV method was employed to evaluate the influence of various potential factors on the strain's antibacterial activity using cost-effective substrates. Twelve variables were considered effective for investigation, with an emphasis on assessing the antibacterial activity against <i>Staphylococcus aureus</i> and <i>Enterococcus faecalis</i> via the micro-dilution method. The findings indicate that a quadratic model and a second-order polynomial equation are statistically significant at the 95% confidence level due to a low P-value (< 0.0001) in this context. As a result, A1BFe+C agar medium, incubated at 28°C for three days with a shaking speed of 200 rpm, using specific concentrations of starch (5.0 g/l), yeast extract (6.0 g/l), peptone (4.0 g/l), KBr (0.5 g/l), CaCO₃ (0.2 g/l), sea salt (15 g/l), and Fe₂(SO₄)₃ (0.003 g/l), along with an inoculum size of 3.0% v/v at a pH of 6, exhibits the enhanced antibacterial activity against <i>Staphylococcus aureus</i> and <i>Enterococcus faecalis</i> reached up to 69 and 166 (Bu/ml). It could be concluded that the MN38 of Caspian Sea sediments was a potent source of antimicrobial agent production and the production process was significantly optimized using mathematical methods.</p>","PeriodicalId":14152,"journal":{"name":"International Journal of Molecular and Cellular Medicine","volume":"14 2","pages":"666-681"},"PeriodicalIF":0.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12321305/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144789040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Innovative Aptamer-HRP Conjugation for Cardiac Troponin I Detection: A Novel ELASA Approach for AMI Diagnostics.","authors":"Mahshid Bahari, Farshid Yeganeh, Ali Kargar","doi":"10.22088/IJMCM.BUMS.14.2.705","DOIUrl":"10.22088/IJMCM.BUMS.14.2.705","url":null,"abstract":"<p><p>Acute myocardial infarction (AMI), a major global cause of mortality, is diagnosed using cardiac troponin I (cTnI). Antibody-based assays face challenges, prompting the exploration of aptamers. This study develops an aptamer-HRP probe and ELASA for improved cTnI detection. Aptamer-based enzyme-linked aptamer assays (ELASA) were developed to detect cTnI using Tro4 and Tro6 aptamers. Molecular docking was performed via the HDOCK web server to confirm aptamer binding affinity to cTnI. Tro4 was biotinylated for use as a capture probe, while Tro6 was conjugated to HRP through sulfo-SMCC crosslinking, followed by size exclusion chromatography and purification. Direct and sandwich ELASA assays were performed using streptavidin-coated plates and clinical serum samples from AMI and non-AMI patients. Data were analyzed using GraphPad Prism10 and SPSS software. Molecular docking confirmed the high binding affinity of Tro4 and Tro6 aptamers to cTnI, with significant interaction energies. Direct ELASA verified aptamer binding, and optimal concentrations were determined as 10μM for Tro4 and 5μM for Tro6. A sandwich ELASA using paired aptamers achieved improved sensitivity and specificity for cTnI detection. The assay displayed a linear response between 0.1-22 ng.mL cTnI (R²=0.94), with a limit of detection (LOD) of 0.10ng.mL. When tested on patient serum samples, results correlated with a commercial antibody-based ELASA kit. This study successfully developed a highly sensitive and specific sandwich ELASA for cTnI detection, utilizing the optimal aptamers Tro4 and Tro6. The results demonstrated excellent sensitivity, specificity, and potential clinical applicability, offering a promising alternative to antibody-based assays.</p>","PeriodicalId":14152,"journal":{"name":"International Journal of Molecular and Cellular Medicine","volume":"14 2","pages":"705-713"},"PeriodicalIF":0.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12321301/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144789038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Candida albicans</i> Impact on the Progression, Morphology, and Cellular Integrity of Biofilm Formation on the Surfaces of Implants; Current Knowledge and Future Perspectives.","authors":"Seçkin Yalçın, Selin Özel, Dilara Çamyar, Emir Farboud Bonabian","doi":"10.22088/IJMCM.BUMS.14.2.620","DOIUrl":"10.22088/IJMCM.BUMS.14.2.620","url":null,"abstract":"<p><p>The capacity of <i>Candida albicans</i> to adhere to diverse oral substrates constitutes a pivotal preliminary phase in the formation of a pathogenic fungal biofilm. Yeast cells demonstrate a considerable ability to bind to host tissues, encompassing dental structures and mucosal surfaces, in addition to synthetic, non-biological materials such as dental appliances. Biomaterials utilized for the restoration of oral functionality are prone to biofilm formation, which can detrimentally affect oral health. Oral microorganisms can adhere to both hydrophobic and hydrophilic surfaces; however, in vivo investigations indicate that hydrophobic surfaces tend to accumulate minimal biofilm due to differential shear forces. Rough surfaces are observed to retain more biofilm compared to their smooth counterparts. The presence of biofilms on composite materials and glass-ionomer cement types results in surface degradation, consequently fostering additional biofilm development. While the leaching of residual monomers from composites has been shown to influence biofilm proliferation in vitro, the effect in vivo appears to be less consequential, likely attributable to the dilution and continual renewal of saliva. Furthermore, research has produced inconsistent findings regarding the influence of fluoride release from glass-ionomer cement types. A comparative analysis is conducted between biomaterial-associated infections in implants and devices situated in other anatomical regions and the formation of oral biofilms. The discourse critically evaluates alterations to biomaterials aimed at diminishing biofilm formation on implants and devices, taking into account their prospective applications within dentistry. The conclusion reached is that for dental applications, antimicrobial coatings that exterminate fungi upon contact are deemed more efficacious than those that gradually release antimicrobial agents.</p>","PeriodicalId":14152,"journal":{"name":"International Journal of Molecular and Cellular Medicine","volume":"14 2","pages":"620-636"},"PeriodicalIF":0.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12321306/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144789069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TNF-α and E-Selectin as Valuable Biomarkers in Patients with Acute Coronary Artery Syndrome.","authors":"Hassan Jabbar Auda, Ali Mohammed Barakat, Hassan Sarhan Sachet, Raed Fanoukh Aboqader, Adnan Taan Thamer","doi":"10.22088/IJMCM.BUMS.14.2.694","DOIUrl":"10.22088/IJMCM.BUMS.14.2.694","url":null,"abstract":"<p><p>Coronary artery disease (CAD) remains the leading cause of mortality worldwide, especially in developing countries, with dyslipidemia being a major risk factor. This study aimed to evaluate lipid parameters and inflammatory biomarkers-E-selectin and tumor necrosis factor-alpha (TNF-α)-to understand their roles in the pathogenesis of acute coronary syndrome (ACS). A case-control design was used, involving 120 participants: 60 patients diagnosed with ACS and 60 healthy controls, enrolled between January and December 2024. Blood samples were analyzed to assess lipid profiles, including total cholesterol, triglycerides, HDL, LDL, and VLDL, using a SMART-120 chemistry analyzer. Serum levels of TNF-α and E-selectin were measured using enzyme-linked immunosorbent assay (ELISA). Results showed significant differences in lipid profiles between ACS patients and controls, supporting the impact of dyslipidemia on ACS development. E-selectin levels were significantly elevated in ACS patients (213.26 ± 2.72 pg/mL) compared to controls (175.11 ± 2.71 pg/mL), with P < 0.0001. Similarly, TNF-α levels were higher in patients (83.20 ± 3.88 pg/mL) than controls (45.65 ± 1.79 pg/mL), also with P < 0.0001. ROC curve analysis demonstrated that E-selectin had 96% sensitivity and specificity at a cutoff of 73.44 pg/mL, while TNF-α had 93% sensitivity and 86% specificity at a cutoff of 188.65 pg/mL. Both biomarkers positively correlated with body mass index (r = 0.572, P < 0.0001).The findings suggest that TNF-α and E-selectin are potential diagnostic biomarkers for ACS and play key <b><i>.</i></b></p>","PeriodicalId":14152,"journal":{"name":"International Journal of Molecular and Cellular Medicine","volume":"14 2","pages":"694-704"},"PeriodicalIF":0.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12321309/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144789074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the Antibacterial and Anti-Biofilm Activity of Chitosan-Arginine Nanoparticles and Sodium Fluoride against <i>Streptococcus</i> <i>mutans</i>.","authors":"Fahimeh Daneshyar, Mohammad Yousef Alikhani, Soudeh Tayebi, Delaram Abedi Firouzjaei","doi":"10.22088/IJMCM.BUMS.14.2.637","DOIUrl":"10.22088/IJMCM.BUMS.14.2.637","url":null,"abstract":"<p><p>Dental caries is among the most prevalent chronic diseases. It arises from bacterial biofilm formation on tooth surfaces due to metabolic activity. <i>Streptococcus</i> <i>mutans</i> (<i>S</i>. <i>mutans</i>) is a key pathogen implicated in the development of dental caries. As bacterial resistance to conventional treatments increases, there is a growing interest in using novel compounds that possess antibacterial and antibiofilm properties. This study evaluated the effect of chitosan-arginine nanoparticles (CS-Arg NPs) and sodium fluoride (NaF) on inhibiting <i>S</i>. <i>mutans</i>' growth. After synthesizing CS-Arg NPs, their size, morphology, and chemical structure were evaluated. The broth microdilution method determined the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of CS-Arg NPs and NaF. The combined antibacterial and antibiofilm effect of CS-Arg NPs and NaF was assessed using the checkerboard method. The CS-Arg NPs had an average size of 269.9 nm with a zeta potential of +38.3 mV. The MIC of <i>S</i>. <i>mutans</i> for CS-Arg NPs and NaF was 312 µg/mL and 625 µg/mL, respectively, and the MBC for these NPs and NaF was 625 µg/mL and 2500 µg/mL, respectively. The fractional inhibitory concentration index (FICI) of the combination of CS-Arg NPs and NaF showed an additive effect (FICI = 1). The inhibitory effect of different concentrations of CS-Arg NPs and NaF, alone or in combination, on biofilm formation in the studied strain ranged from approximately 12% to 81%. This study demonstrated that CS-Arg NPs have antibacterial and antibiofilm properties against <i>S</i>. <i>mutans</i>, and their combination with NaF can enhance these antibacterial effects. These findings suggest that CS-Arg NPs and NaF, as a novel combination, could effectively develop oral hygiene products.</p>","PeriodicalId":14152,"journal":{"name":"International Journal of Molecular and Cellular Medicine","volume":"14 2","pages":"637-645"},"PeriodicalIF":0.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12321303/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144789037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Colorectal Cancer; Novel Approaches in Chimeric Antigen Receptors (CAR) -T cell.","authors":"Mahyar Haki, Mohammad Darvishi","doi":"10.22088/IJMCM.BUMS.14.2.777","DOIUrl":"10.22088/IJMCM.BUMS.14.2.777","url":null,"abstract":"<p><p>Colorectal cancer (CRC) is a popular type of cancer, characterized by high mortality and a notable impression on the well-being of individuals. The success of adoptive chimeric antigen receptor T (CAR-T) cell therapy in treating hematological malignancies has been remarkable in recent years; however, its application in solid tumors like CRC has many challenges. These obstacles encompass the immunosuppressive microenvironment of the tumor, the insufficient targeting of CAR-T cells, the limited lifespan of CAR-T cells within the body, and the constrained capacity for proliferation. Additionally, CAR-T cells face hurdles in effectively infiltrating the tumor site, which further complicates treatment outcomes. Diverse innovative strategies have been suggested to surmount these barriers in the context of CRC. This comprehensive review endeavors to meticulously elucidate an exhaustive and detailed evaluation of the prevailing and contemporary landscape concerning CAR-T cell therapy as it pertains to the intricate management of CRC, while simultaneously offering a thorough indication of the various risk factors and the associated prevalence that are intricately linked with the manifestation and progression of CRC.</p>","PeriodicalId":14152,"journal":{"name":"International Journal of Molecular and Cellular Medicine","volume":"14 2","pages":"777-792"},"PeriodicalIF":0.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12321304/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144789071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SARS-CoV-2 ORF7 subgenomic RNA and Host IFN-β Expression in COVID-19 Hospitalized Patients with Different Prognosis.","authors":"Akbar Zirakjou, Farzin Sadeghi, Arefeh Ebrahimian Shiadeh, Maryam Hatamibardar, Mohammad Ranaee, Hemmat Gholinia, Hossein Ghorbani","doi":"10.22088/IJMCM.BUMS.14.2.726","DOIUrl":"10.22088/IJMCM.BUMS.14.2.726","url":null,"abstract":"<p><p>The worldwide spread of COVID-19, triggered by SARS-CoV-2, has highlighted how viral accessory proteins contribute significantly to bypassing host immune defenses and increasing illness severity. This study investigates the relationship between the levels of SARS-CoV-2 subgenomic RNA (sgRNA) for ORF7a and ORF7b and host interferon-beta (IFN-β) expression in hospitalized COVID-19 patients with different prognoses. Upper respiratory tract samples from 89 patients (49 with poor prognosis and 40 with good prognosis) were analyzed using quantitative real-time PCR to measure ORF7a, ORF7b, and IFN-β expression levels. The results revealed significantly higher expression of ORF7a and ORF7b in patients with poor prognosis compared to those with favorable outcomes (P < 0.001). Conversely, IFN-β expression was significantly reduced in the poor prognosis group (P < 0.001), suggesting a potential mechanism of immune suppression. Older age, underlying health conditions, and elevated levels of inflammatory biomarkers, such as CRP and D-dimer, were also associated with poorer outcomes. These findings underscore the potential role of ORF7 proteins in suppressing IFN-β signaling, contributing to disease severity. Targeting these viral proteins may offer promising therapeutic avenues to enhance antiviral responses and improve patient outcomes. The study was conducted from August 2022 to February 2022. Further research is warranted to better understand the interplay between viral immune evasion mechanisms and host responses across diverse patient populations.</p>","PeriodicalId":14152,"journal":{"name":"International Journal of Molecular and Cellular Medicine","volume":"14 2","pages":"726-735"},"PeriodicalIF":0.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12321312/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144789072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Osteogenic potential of human dental pulp stem cells, and human dermal fibroblasts exposed hydroxyapatite nanoparticles: A comparative in vitro study.","authors":"Mohsen Naseri, Sepideh Sarfi, Mohammad Yahya Hanafi-Bojd, Ehsaneh Azaryan","doi":"10.22088/IJMCM.BUMS.14.2.656","DOIUrl":"10.22088/IJMCM.BUMS.14.2.656","url":null,"abstract":"<p><p>This study aimed to evaluate the osteogenic response of human dermal fibroblasts (hDFs) and human dental pulp stem cells (hDPSCs) when exposed to hydroxyapatite nanoparticles (HA-NPs), which are commonly employed biomaterials in the field of bone regeneration. The sol-gel method was employed to synthesize HA-NPs. Cell viability was assessed using the MTT assay after 24 and 72 hours of exposure to HA-NPs. Osteogenic differentiation was assessed through Alizarin red S staining (ARS), alkaline phosphatase (ALP) activity assay, and quantitative real-time PCR (qPCR) to evaluate the expression levels of osteogenic markers, including BMP-2, VEGF-A, RUNX2, and IL-6. The HA-NPs had a nanorod shape, with dimensions of 17-29 nm in width and 62-89 nm in length. Both hDPSCs and hDFs demonstrated enhanced osteogenic potential when exposed to HA-NPs, as indicated by increased ARS staining for calcium deposition and elevated ALP activity. Gene expression analysis showed up-regulation of BMP-2 and VEGF-A and down-regulation of RUNX2 in both cell types. IL-6 expression markedly increased in hDFs but did not show significant changes in hDPSCs compared to the control group. The findings of this study suggest that HA-NPs may enhance the osteogenic capability of hDPSCs and hDFs. The results demonstrate that while fibroblasts exhibit some mineralization potential, hDPSCs are the most suitable cell type for bone regenerative applications.</p>","PeriodicalId":14152,"journal":{"name":"International Journal of Molecular and Cellular Medicine","volume":"14 2","pages":"656-665"},"PeriodicalIF":0.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12321310/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144789042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}